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 嘔(ou)吐(tu)(tu)毒(du)(du)素為(wei)白(bai)(bai)色針狀結晶，熔點(dian)為(wei)151 ~153℃（醋酸乙(yi)基(ji)石(shi)油(you)）。不(bu)飽和酮(tong)基(ji)的(de)存在(zai)使(shi)得其在(zai)短波紫外(wai)下有(you)吸收峰，但與許(xu)多其它物(wu)(wu)質在(zai)此處(chu)的(de)紫外(wai)吸收峰相重疊，屬非特征性的(de)。紫外(wai)輻射下不(bu)顯熒光。DON易(yi)溶于極性的(de)溶劑如水、甲(jia)醇(chun)(chun)、乙(yi)醇(chun)(chun)、乙(yi)腈(jing)、丙酮(tong)和乙(yi)酸乙(yi)酯，不(bu)溶于正已烷、丁(ding)醇(chun)(chun)、石(shi)油(you)醚(mi)。鼠(shu)抗(kang)(kang)(kang)(kang)嘔(ou)吐(tu)(tu)毒(du)(du)素單(dan)克(ke)隆抗(kang)(kang)(kang)(kang)體(ti)說(shuo)明(ming)(ming)書(shu)【中(zhong)文(wen)名(ming)稱】鼠(shu)抗(kang)(kang)(kang)(kang)嘔(ou)吐(tu)(tu)毒(du)(du)素單(dan)克(ke)隆抗(kang)(kang)(kang)(kang)體(ti)【英文(wen)名(ming)稱】Mouse Anti-vomiting Toxin Monoclonal Antibody【貨(huo)(huo)    號】DM-Ab0180【來    源】Balb/c 小鼠(shu)【亞    型】IgG  【物(wu)(wu)理性狀】液體(ti)，避(bi)(bi)免(mian)反(fan)復(fu)凍融(rong)(rong)(rong)【ELISA 效(xiao)(xiao)價】 0.005M PBS 體(ti)系(xi)下1:200000 以(yi)上 【IC50 】30ppb（μg/Kg）【緩(huan)沖(chong)液】0.01M PBS PH7.4  不(bu)加防腐劑【儲存及有(you)效(xiao)(xiao)期(qi)】-20℃以(yi)下3 年，避(bi)(bi)免(mian)反(fan)復(fu)凍融(rong)(rong)(rong)【相關產品】鼠(shu)抗(kang)(kang)(kang)(kang)黃(huang)曲(qu)霉(mei)毒(du)(du)素B1單(dan)克(ke)隆抗(kang)(kang)(kang)(kang)體(ti)；鼠(shu)抗(kang)(kang)(kang)(kang)黃(huang)曲(qu)霉(mei)毒(du)(du)素總量單(dan)克(ke)隆抗(kang)(kang)(kang)(kang)體(ti)；鼠(shu)抗(kang)(kang)(kang)(kang)黃(huang)曲(qu)霉(mei)毒(du)(du)素M1單(dan)克(ke)隆抗(kang)(kang)(kang)(kang)體(ti) 嘔(ou)吐(tu)(tu)毒(du)(du)素偶(ou)聯(lian)免(mian)疫全抗(kang)(kang)(kang)(kang)原(yuan)說(shuo)明(ming)(ming)書(shu)【中(zhong)文(wen)名(ming)稱】嘔(ou)吐(tu)(tu)毒(du)(du)素偶(ou)聯(lian)全抗(kang)(kang)(kang)(kang)原(yuan)【英文(wen)名(ming)稱】Vomitoxin-Protein Conjugate【貨(huo)(huo)    號】DM-Ag0180【蛋白(bai)(bai)載體(ti)】牛(niu)血清白(bai)(bai)蛋白(bai)(bai)/卵清白(bai)(bai)蛋白(bai)(bai)/匙孔(kong)血藍(lan)蛋白(bai)(bai)BSA/OVA/KLH【物(wu)(wu)理性狀】液體(ti)，避(bi)(bi)免(mian)反(fan)復(fu)凍融(rong)(rong)(rong)【ELISA效(xiao)(xiao)價】 0.005M PBS 體(ti)系(xi)下1:30000以(yi)上【免(mian)疫層析(xi)效(xiao)(xiao)價】 1:40以(yi)上，偶(ou)聯(lian)比大于1:60 【免(mian)疫原(yuan)偶(ou)聯(lian)位點(dian)】3-OH √ /15-0H【蛋白(bai)(bai)濃度】5.0 mg/ml【緩(huan)沖(chong)液】0.01M PBS PH7.4無防腐劑【儲存條件】-20℃以(yi)下【有(you)效(xiao)(xiao)期(qi)】2年，避(bi)(bi)免(mian)反(fan)復(fu)凍融(rong)(rong)(rong) 

菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)說(shuo)(shuo)明(ming)(ming) 提(ti)示：請嚴格(ge)(ge)按(an)(an)照說(shuo)(shuo)明(ming)(ming)書操(cao)(cao)作(zuo),如(ru)未按(an)(an)說(shuo)(shuo)明(ming)(ming)書操(cao)(cao)作(zuo)，本中心不(bu)承擔售后(hou)。 本中心目前有(you)(you)標準菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)（斜(xie)面、甘(gan) 油(you)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)、穿刺(ci)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)和(he)凍(dong)(dong)干(gan)(gan)(gan)粉(fen)四種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)形(xing)式(shi)）、基因(yin)工(gong)程菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)（斜(xie)面、甘(gan)油(you) 菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)和(he)穿刺(ci)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)三種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)形(xing)式(shi)）、食用益(yi)生菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)和(he)工(gong) 業發酵菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，基本涵蓋了環(huan)境、工(gong)業、農(nong)業、分析、食品(pin)、藥(yao) 用真菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)等各類生產和(he)科研微生物。 一(yi) 菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)簡介(jie) 菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)名稱 牙齦卟啉(lin)單胞菌(jun)(jun)(jun)(jun)(jun)(jun)(jun) 編號 ATCC33277 規格(ge)(ge) 培(pei)(pei)(pei)(pei)養(yang)(yang)皿(min)(min) 菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)主要應(ying)用 二(er) 保(bao)存(cun)(cun)(cun)條件(jian)(jian) 斜(xie)面、穿刺(ci)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)和(he)凍(dong)(dong)干(gan)(gan)(gan)粉(fen)可(ke)(ke)常(chang)溫(wen)運輸，但(dan)應(ying)在4-10度(du)(du)(du)長(chang)(chang)期保(bao)存(cun)(cun)(cun)。甘(gan)油(you)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)可(ke)(ke)常(chang)溫(wen)運輸，但(dan)應(ying)在-80度(du)(du)(du)長(chang)(chang)期 保(bao)存(cun)(cun)(cun)。微生物菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)應(ying)保(bao)存(cun)(cun)(cun)于(yu)低溫(wen)、清潔(jie)和(he)干(gan)(gan)(gan)燥的(de)(de)地(di)方，室(shi)(shi)溫(wen)放置(zhi)(zhi)時(shi)(shi)間(jian)過(guo)(guo)長(chang)(chang)會(hui)導致菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)衰退(tui)(tui)。 三 培(pei)(pei)(pei)(pei)養(yang)(yang)條件(jian)(jian) 培(pei)(pei)(pei)(pei)養(yang)(yang)基 血(xue)瓊脂平(ping)板 培(pei)(pei)(pei)(pei)養(yang)(yang)溫(wen)度(du)(du)(du) 37 度(du)(du)(du) 培(pei)(pei)(pei)(pei)養(yang)(yang)時(shi)(shi)間(jian) 2-5 天(tian) 培(pei)(pei)(pei)(pei)養(yang)(yang)條件(jian)(jian) 嚴格(ge)(ge)厭氧 四 活化步驟(zou) 培(pei)(pei)(pei)(pei)養(yang)(yang)皿(min)(min)培(pei)(pei)(pei)(pei)養(yang)(yang)物首(shou)次傳代(dai)，先用75%酒(jiu)精將斜(xie)面硅膠塞(sai)處擦拭，然(ran)后(hou)酒(jiu)精燈旁燒一(yi)下(xia)消毒，然(ran)后(hou)開啟硅膠塞(sai)， 用接(jie)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)針(zhen)（細菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)類）或接(jie)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)鏟（霉(mei)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)類）取一(yi)點菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，然(ran)后(hou)接(jie)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)到新的(de)(de)斜(xie)面試管上（劃線涂布都(dou)可(ke)(ke)以(yi)）。 也可(ke)(ke)以(yi)接(jie)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)到液體培(pei)(pei)(pei)(pei)養(yang)(yang)基中做(zuo)搖菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)養(yang)(yang)。剩余(yu)的(de)(de)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)可(ke)(ke)以(yi)按(an)(an)照保(bao)存(cun)(cun)(cun)條件(jian)(jian)放到相(xiang)應(ying)條件(jian)(jian)保(bao)存(cun)(cun)(cun)。如(ru)果有(you)(you)不(bu)明(ming)(ming)白 之處，應(ying)先與我中心聯(lian)系(xi)，避免不(bu)必要的(de)(de)損失(shi)(4009681786,微信：)。 五 注意事(shi)項 1 微生物菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)應(ying)保(bao)存(cun)(cun)(cun)于(yu)低溫(wen)、清潔(jie)和(he)干(gan)(gan)(gan)燥的(de)(de)地(di)方，室(shi)(shi)溫(wen)放置(zhi)(zhi)時(shi)(shi)間(jian)過(guo)(guo)長(chang)(chang)會(hui)導致菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)衰退(tui)(tui)； 2 菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)操(cao)(cao)作(zuo)在無(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)條件(jian)(jian)下(xia)進行(xing)，接(jie)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)完畢(bi)應(ying)該滅菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)再做(zuo)丟棄處理(li)； 3 斜(xie)面菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)穿刺(ci)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)存(cun)(cun)(cun)時(shi)(shi)間(jian)通常(chang) 為(wei) 3-6 個月，應(ying)根據菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)狀況及時(shi)(shi)結轉；凍(dong)(dong)干(gan)(gan)(gan)粉(fen)保(bao)存(cun)(cun)(cun)時(shi)(shi)間(jian)通常(chang)是2-25年(nian)；甘(gan)油(you)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)保(bao)存(cun)(cun)(cun)時(shi)(shi)間(jian)為(wei)2-5年(nian)

大鼠(shu)血(xue)(xue)管內(nei)(nei)(nei)(nei)(nei)皮細(xi)胞(bao)生(sheng)長因(yin)(yin)子(zi)A(VEGF-A)ELISA試(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)中(zhong)(zhong)(zhong)(zhong)文名稱大鼠(shu)血(xue)(xue)管內(nei)(nei)(nei)(nei)(nei)皮細(xi)胞(bao)生(sheng)長因(yin)(yin)子(zi)A(VEGF-A)ELISA試(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)規格(ge)96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)類(lei)型(xing)血(xue)(xue)清、血(xue)(xue)漿、細(xi)胞(bao)上(shang)(shang)清液(ye)(ye)(ye)、尿液(ye)(ye)(ye)、體液(ye)(ye)(ye)、灌洗(xi)液(ye)(ye)(ye)等使(shi)用(yong)范圍科(ke)研實(shi)(shi)驗(yan)，不(bu)(bu)得用(yong)于(yu)臨床診(zhen)斷反應(ying)(ying)(ying)時(shi)間(jian)1-5h試(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)保(bao)存(cun)(cun)(cun)(cun)(cun)2-8℃有(you)(you)效期6個(ge)(ge)月貨期現貨【操作步(bu)驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)與(yu)(yu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被板(ban)(ban)(ban)(ban)(ban)(ban)上(shang)(shang)設標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)一(yi)、第(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)100μl，然后(hou)(hou)(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)一(yi)、第(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)；然后(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)一(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)100μl分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)；然后(hou)(hou)(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)先(xian)各(ge)(ge)取(qu)50μl棄掉，再(zai)(zai)各(ge)(ge)取(qu)50μl分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)50ul，混(hun)(hun)勻(yun)；混(hun)(hun)勻(yun)后(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)50μl分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)后(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)取(qu)50μl加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)九(jiu)(jiu)、第(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)九(jiu)(jiu)第(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)后(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)九(jiu)(jiu)第(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)50μl棄掉。（稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)量都(dou)為(wei)(wei)50μl，濃(nong)度(du)(du)(du)(du)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)為(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)設空(kong)(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)(kong)白對(dui)(dui)照孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)及酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)劑(ji)(ji)(ji)(ji)(ji)，其(qi)(qi)余各(ge)(ge)步(bu)操作相(xiang)同(tong)(tong)(tong)）、待(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被板(ban)(ban)(ban)(ban)(ban)(ban)上(shang)(shang)待(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)40μl，然后(hou)(hou)(hou)(hou)(hou)再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)待(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)＊終稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)度(du)(du)(du)(du)為(wei)(wei)5倍(bei)(bei)(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)將樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底(di)部(bu)，盡(jin)量不(bu)(bu)觸(chu)及孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕輕晃(huang)動混(hun)(hun)勻(yun)。3. 溫(wen)(wen)育：用(yong)封(feng)板(ban)(ban)(ban)(ban)(ban)(ban)膜封(feng)板(ban)(ban)(ban)(ban)(ban)(ban)后(hou)(hou)(hou)(hou)(hou)置(zhi)37℃溫(wen)(wen)育30分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)。4. 配液(ye)(ye)(ye)：將30（48T的(de)(de)(de)(de)(de)(de)(de)(de)(de)20倍(bei)(bei)(bei)(bei)）倍(bei)(bei)(bei)(bei)濃(nong)縮洗(xi)滌(di)(di)液(ye)(ye)(ye)用(yong)蒸(zheng)餾水30（48T的(de)(de)(de)(de)(de)(de)(de)(de)(de)20倍(bei)(bei)(bei)(bei)）倍(bei)(bei)(bei)(bei)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)備(bei)用(yong)。5. 洗(xi)滌(di)(di)：小心揭掉封(feng)板(ban)(ban)(ban)(ban)(ban)(ban)膜，棄去(qu)液(ye)(ye)(ye)體，甩干，每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)滿洗(xi)滌(di)(di)液(ye)(ye)(ye)，靜置(zhi)30秒后(hou)(hou)(hou)(hou)(hou)棄去(qu)，如此重復5次(ci)(ci)，拍干。6. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)(mei)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)劑(ji)(ji)(ji)(ji)(ji)50μl，空(kong)(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外。7. 溫(wen)(wen)育：操作同(tong)(tong)(tong)3。8. 洗(xi)滌(di)(di)：操作同(tong)(tong)(tong)5。9. 顯(xian)(xian)色(se)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入顯(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)A50μl，再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入顯(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)B50μl，輕輕震蕩(dang)混(hun)(hun)勻(yun)，37℃避(bi)光(guang)顯(xian)(xian)色(se)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong). 10. 終止：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終止液(ye)(ye)(ye)50μl，終止反應(ying)(ying)(ying)（此時(shi)藍色(se)立轉黃色(se)）。11. 測(ce)(ce)(ce)定(ding)(ding)(ding)：以(yi)空(kong)(kong)白空(kong)(kong)調零，450nm波長依(yi)序測(ce)(ce)(ce)量各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)吸光(guang)度(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)定(ding)(ding)(ding)應(ying)(ying)(ying)在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終止液(ye)(ye)(ye)后(hou)(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)以(yi)內(nei)(nei)(nei)(nei)(nei)進行(xing)。【注意事項】1． 試(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)從(cong)冷藏環(huan)境(jing)中(zhong)(zhong)(zhong)(zhong)取(qu)出應(ying)(ying)(ying)在(zai)(zai)室(shi)溫(wen)(wen)平(ping)衡15-30分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)后(hou)(hou)(hou)(hou)(hou)方(fang)可使(shi)用(yong)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被板(ban)(ban)(ban)(ban)(ban)(ban)開封(feng)后(hou)(hou)(hou)(hou)(hou)如未用(yong)完(wan)，板(ban)(ban)(ban)(ban)(ban)(ban)條應(ying)(ying)(ying)裝(zhuang)入密封(feng)袋中(zhong)(zhong)(zhong)(zhong)保(bao)存(cun)(cun)(cun)(cun)(cun)。2． 濃(nong)洗(xi)滌(di)(di)液(ye)(ye)(ye)可能會有(you)(you)結(jie)晶析出，稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)時(shi)可在(zai)(zai)水浴中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)溫(wen)(wen)助溶，洗(xi)滌(di)(di)時(shi)不(bu)(bu)影(ying)(ying)響結(jie)果。3． 各(ge)(ge)步(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)均應(ying)(ying)(ying)使(shi)用(yong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)器，并(bing)經(jing)(jing)常(chang)校對(dui)(dui)其(qi)(qi)準(zhun)(zhun)(zhun)(zhun)確性，以(yi)避(bi)免(mian)試(shi)驗(yan)誤(wu)差(cha)。一(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)時(shi)間(jian)＊好控制在(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)內(nei)(nei)(nei)(nei)(nei)，如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)數(shu)(shu)量多，推(tui)薦使(shi)用(yong)排槍(qiang)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)。4． 請每(mei)次(ci)(ci)測(ce)(ce)(ce)定(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)(tong)(tong)時(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)，＊好做復孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)待(dai)測(ce)(ce)(ce)物(wu)(wu)質含量過高(gao)(gao)（樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)OD值(zhi)(zhi)(zhi)(zhi)大于(yu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)(di)一(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)），請先(xian)用(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)一(yi)定(ding)(ding)(ding)倍(bei)(bei)(bei)(bei)數(shu)(shu)（n倍(bei)(bei)(bei)(bei)）后(hou)(hou)(hou)(hou)(hou)再(zai)(zai)測(ce)(ce)(ce)定(ding)(ding)(ding)，計算(suan)時(shi)請＊后(hou)(hou)(hou)(hou)(hou)乘以(yi)總稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)倍(bei)(bei)(bei)(bei)數(shu)(shu)（×n×5）。5． 封(feng)板(ban)(ban)(ban)(ban)(ban)(ban)膜只限一(yi)次(ci)(ci)性使(shi)用(yong)，以(yi)避(bi)免(mian)交(jiao)叉(cha)(cha)(cha)(cha)污染。6． 底(di)物(wu)(wu)請避(bi)光(guang)保(bao)存(cun)(cun)(cun)(cun)(cun)。7． 嚴格(ge)按照說明書(shu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)操作進行(xing)，試(shi)驗(yan)結(jie)果判定(ding)(ding)(ding)必(bi)(bi)須以(yi)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀數(shu)(shu)為(wei)(wei)準(zhun)(zhun)(zhun)(zhun).8． 所(suo)有(you)(you)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)，洗(xi)滌(di)(di)液(ye)(ye)(ye)和各(ge)(ge)種廢棄物(wu)(wu)都(dou)應(ying)(ying)(ying)按傳(chuan)染物(wu)(wu)處理(li)。9． 本(ben)(ben)(ben)試(shi)劑(ji)(ji)(ji)(ji)(ji)不(bu)(bu)同(tong)(tong)(tong)批(pi)(pi)(pi)號組分(fen)(fen)(fen)(fen)(fen)(fen)不(bu)(bu)得混(hun)(hun)用(yong)。10. 如與(yu)(yu)英(ying)文說明書(shu)有(you)(you)異，以(yi)英(ying)文說明書(shu)為(wei)(wei)準(zhun)(zhun)(zhun)(zhun)。【計算(suan)】 以(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)(du)為(wei)(wei)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)為(wei)(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，    在(zai)(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)繪出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)，根據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)OD      值(zhi)(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)查出相(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)(du)；再(zai)(zai)乘以(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)      倍(bei)(bei)(bei)(bei)數(shu)(shu)；或用(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)(du)與(yu)(yu)OD值(zhi)(zhi)(zhi)(zhi)計算(suan)出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)      準(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)回歸方(fang)程式，將樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)      代入方(fang)程式，計算(suan)出樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)濃(nong)度(du)(du)(du)(du)，再(zai)(zai)乘以(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)      倍(bei)(bei)(bei)(bei)數(shu)(shu)，即為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際濃(nong)度(du)(du)(du)(du)。                   【試(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)組成】試(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)組成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)保(bao)存(cun)(cun)(cun)(cun)(cun)說明書(shu)1份1份密封(feng)袋1個(ge)(ge)1個(ge)(ge)封(feng)板(ban)(ban)(ban)(ban)(ban)(ban)膜12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條2片（96）2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)1.5ml×1瓶(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)劑(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)6 ml×1瓶(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)3 ml×1瓶(ping)(ping)6 ml×1瓶(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)顯(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)3 ml×1瓶(ping)(ping)6 ml×1瓶(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)顯(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)3 ml×1瓶(ping)(ping)6 ml×1瓶(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)終止液(ye)(ye)(ye)3ml×1瓶(ping)(ping)6ml×1瓶(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)注意：使(shi)用(yong)前(qian)請檢(jian)查試(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)中(zhong)(zhong)(zhong)(zhong)試(shi)劑(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和數(shu)(shu)量與(yu)(yu)表格(ge)是(shi)否一(yi)致。【精密度(du)(du)(du)(du)】精密度(du)(du)(du)(du)用(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)測(ce)(ce)(ce)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)變(bian)異系數(shu)(shu)CV 表示。CV（%） = SD/mean×100批(pi)(pi)(pi)內(nei)(nei)(nei)(nei)(nei)差(cha)：取(qu)同(tong)(tong)(tong)批(pi)(pi)(pi)次(ci)(ci)試(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)對(dui)(dui)低、中(zhong)(zhong)(zhong)(zhong)、高(gao)(gao)值(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)進行(xing)定(ding)(ding)(ding)量檢(jian)測(ce)(ce)(ce)，每(mei)份樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)連續(xu)測(ce)(ce)(ce)定(ding)(ding)(ding)20 次(ci)(ci)，分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)計算(suan)不(bu)(bu)同(tong)(tong)(tong)濃(nong)度(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)平(ping)均值(zhi)(zhi)(zhi)(zhi)及SD 值(zhi)(zhi)(zhi)(zhi)。批(pi)(pi)(pi)內(nei)(nei)(nei)(nei)(nei)差(cha)： CV<9%批(pi)(pi)(pi)間(jian)差(cha)：選取(qu)3 個(ge)(ge)不(bu)(bu)同(tong)(tong)(tong)批(pi)(pi)(pi)次(ci)(ci)的(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)對(dui)(dui)低、中(zhong)(zhong)(zhong)(zhong)、高(gao)(gao)值(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)進行(xing)定(ding)(ding)(ding)量測(ce)(ce)(ce)定(ding)(ding)(ding)，每(mei)個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)使(shi)用(yong)同(tong)(tong)(tong)一(yi)試(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)重復測(ce)(ce)(ce)定(ding)(ding)(ding)10 次(ci)(ci)，分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)計算(suan)不(bu)(bu)同(tong)(tong)(tong)濃(nong)度(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)平(ping)均值(zhi)(zhi)(zhi)(zhi)及SD 值(zhi)(zhi)(zhi)(zhi)。批(pi)(pi)(pi)間(jian)差(cha)： CV<11% 【特異性】本(ben)(ben)(ben)試(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)識別(bie)(bie)(bie)天(tian)然和重組微(wei)生(sheng)物(wu)(wu)硝酸(suan)鹽還原酶(mei)(mei)(mei)（Nar），經(jing)(jing)檢(jian)測(ce)(ce)(ce)與(yu)(yu)其(qi)(qi)它相(xiang)似物(wu)(wu)質無明顯(xian)(xian)交(jiao)叉(cha)(cha)(cha)(cha)反應(ying)(ying)(ying)。由(you)于(yu)受到(dao)技術及樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)來(lai)源的(de)(de)(de)(de)(de)(de)(de)(de)(de)限制，不(bu)(bu)可能完(wan)成所(suo)有(you)(you)相(xiang)關或相(xiang)似物(wu)(wu)質交(jiao)叉(cha)(cha)(cha)(cha)反應(ying)(ying)(ying)檢(jian)測(ce)(ce)(ce)，因(yin)(yin)此本(ben)(ben)(ben)試(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)有(you)(you)可能與(yu)(yu)未經(jing)(jing)檢(jian)測(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)其(qi)(qi)它物(wu)(wu)質有(you)(you)交(jiao)叉(cha)(cha)(cha)(cha)反應(ying)(ying)(ying)。 【穩定(ding)(ding)(ding)性】試(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)在(zai)(zai)有(you)(you)效期內(nei)(nei)(nei)(nei)(nei)務(wu)必(bi)(bi)按推(tui)薦溫(wen)(wen)度(du)(du)(du)(du)保(bao)存(cun)(cun)(cun)(cun)(cun)，活性降低率小于(yu)5%。為(wei)(wei)減小外部(bu)因(yin)(yin)素對(dui)(dui)試(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)破壞前(qian)后(hou)(hou)(hou)(hou)(hou)檢(jian)測(ce)(ce)(ce)值(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)影(ying)(ying)響，實(shi)(shi)驗(yan)室(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)環(huan)境(jing)條件需盡(jin)量保(bao)持一(yi)致，尤其(qi)(qi)是(shi)實(shi)(shi)驗(yan)室(shi)內(nei)(nei)(nei)(nei)(nei)溫(wen)(wen)度(du)(du)(du)(du)、濕度(du)(du)(du)(du)及溫(wen)(wen)育條件。其(qi)(qi)次(ci)(ci)由(you)同(tong)(tong)(tong)一(yi)實(shi)(shi)驗(yan)員(yuan)來(lai)進行(xing)操作可減少人(ren)為(wei)(wei)誤(wu)差(cha)。

CTCF Monoclonal Antibody Catalog No  DM-00964 Isotype IgG Reactivity Human;Monkey Applications WB;ELISA Gene Name CTCF Protein Name Transcriptional repressor CTCFImmunogen Purified recombinant fragment of human CTCF expressed in E. Coli. Specificity CTCF Monoclonal Antibody detects endogenous levels of CTCF protein. Formulation Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol. Source Monoclonal, Mouse Purification Affinity purificationDilution Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. Concentration 1 mg/ml Purity ≥90% Storage Stability -20°C/1 year Synonyms CTCF; Transcriptional repressor CTCF; 11-zinc finger protein; CCCTC-binding factor; CTCFL paralog Observed Band Cell Pathway Nucleus, nucleoplasm . Chromosome . Chromosome, centromere . May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase. Tissue Specificity Ubiquitous. Absent in primary spermatocytes. Function function:Transcriptional repressor binding to promoters of vertebrate c-myc gene. Also binds to the PLK and PIM1 promoters. May prevent the access of transcriptional activators to enhancers. Also acts as a transcriptional activator of APP. Involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Seems to act as tumor suppressor.,similarity:Belongs to the CTCF zinc-finger protein family.,similarity:Contains 11 C2H2-type zinc fingers.,subunit:Interacts with CHD8.,tissue specificity:Ubiquitous. Absent in primary spermatocytes.,Background This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combination

ZN143 rabbit pAb Catalog No DM-09315 Isotype IgGReactivity Human;Mouse;Rat Applications WB Gene Name ZNF143 SBF STAF Protein Name Zinc finger protein 143 (SPH-binding factor) (Selenocysteine tRNA gene transcription-activating factor) (hStaf) Immunogen Synthesized peptide derived from human ZN143 AA range: 518-568 Specificity This antibody detects endogenous levels of human ZN143 Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgG Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. Dilution WB 1:1000-2000 Concentration 1 mg/ml Purity ≥90% Storage Stability -20°C/1 year Synonyms Observed Band Cell Pathway Nucleus . Tissue Specificity Expressed in all tissues tested, with the strongest expression in ovary. Function Background matters needing attention Avoid repeated freezing and thawing! Usage suggestions This product can be used in immunological reaction related experiments. For more information, please consult technical personnel.

 Elf-1 Polyclonal Antibody Catalog No DM-01689 Isotype IgGReactivity Human Applications WB;IHC;IF;ELISA Gene Name ELF1 Protein Name ETS-related transcription factor Elf-1 Immunogen The antiserum was produced against synthesized peptide derived from human ELF1. AA range:537-586 Specificity Elf-1 Polyclonal Antibody detects endogenous levels of Elf-1 protein. Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgG Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Dilution Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. Concentration 1 mg/ml Purity ≥90% Storage Stability -20°C/1 year Synonyms ELF1; ETS-related transcription factor Elf-1; E74-like factor 1 Observed Band 67kD Cell Pathway Nucleus. Tissue Specificity In fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis. Function function:Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,subunit:Binds to the underphosphorylated form of RB. May interact with other transcription factors in order to regulate specific genes. Interacts with RUNX1.,tissue specificity:In fetal tissue, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it

Kinase experiment [1]: Preparation Method Prior to use, the [8-14C] adenosine was checked for purity using isocratic HPLC elution. The assay incubation mixture contained 0.4 IU of enzyme in 50 L. The metabolites were separated by thin-layer chromatography. The radioactivity was quantitated by cutting the plastic backed TLC plates and placing them in scintillation vials, and counting in a Packard 2000 CA scintillation counter Applications IC50: 0.15 (HIV-1, A012 isolate), 0.20 μM (HIV-1, A018 isolate). 3-Deazaadenosine (hydrochloride) is an inhibitor of S-adenosylhomocysteine hydrolase, with a Ki of 3.9 μM. Cell experiment [2]: Cell lines Mouse macrophage RAW 264.7 Preparation Method RAW 264.7 cells were pretreated with or without 3-Deazaadenosine (100 μMM) for 1 h, and stimulated by the addition of LPS (1 μg/ml). After incubation for 1 h, the cells were washed, and p65 was recovered by immunoprecipitation with anti-p65 and protein A-Sepharose. Reaction Conditions 0-100 μM 3-Deazaadenosine for 1 hour Applications 3-Deazaadenosine (1-100 ?M) inhibits LPS-induced expression of TNF-α mRNA, increases DNA binding activity of NF-κB, and causes proteolytic degradation of IκBα, but Not IκBβ in RAW 264.7 cells. 3-Deazaadenosine (100 μM) enhances nuclear translocation of NF-κB, but blocks LPS-induced NF-κB transcriptional activity, and such inhibition is augmented by the addition of homocysteine. Animal experiment [3]: Animal models Male, healthy Sprague–Dawley rats (300?50 g) Preparation Method Animals and balloon injury: rats were fed for 5 days prior and 14 days after the balloon injury with standard chow containing c3Ado at a concentration of 10 mg/kg 3-Deazaadenosine body weight. Dosage form 10 mg/kg 3-Deazaadenosine for 5 days prior and 14 days after the balloon injury Applications 3-deazaadenosine (c3Ado) inhibits atherogenesis in mice. Sprague Dawley rats underwent balloon angioplasty. C3Ado was administered orally, starting 5 days prior to the balloon injury and continued for 2 weeks. Fourteen days afte

 小(xiao)鼠(shu)胰島(dao)素(su)(su)(su)(su)(su)樣(yang)(yang)(yang)(yang)(yang)生(sheng)長(chang)(chang)因子(zi) 1（IGF-1）實(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)原理   試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)采(cai)(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙抗(kang)(kang)體(ti)(ti)一(yi)(yi)(yi)步夾心法(fa)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯免(mian)疫吸(xi)(xi)附試(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)（ELISA）。往預先(xian)(xian)包(bao)被脂肪酸結(jie)合(he)(he)蛋(dan)白(bai)(bai)1(FABP1)捕獲抗(kang)(kang)體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包(bao)被微孔(kong)(kong)中(zhong)(zhong)，依(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)測(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)體(ti)(ti)，經過(guo)(guo)(guo)(guo)溫(wen)育并(bing)(bing)徹底(di)(di)洗(xi)(xi)滌(di)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)TMB顯(xian)色(se)，TMB在(zai)(zai)過(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)下轉(zhuan)(zhuan)化(hua)(hua)成藍色(se)，并(bing)(bing)在(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作用(yong)(yong)(yong)(yong)(yong)(yong)(yong)下轉(zhuan)(zhuan)化(hua)(hua)成＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)色(se)。顏色(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂肪酸結(jie)合(he)(he)蛋(dan)白(bai)(bai)1(FABP1)呈正相(xiang)(xiang)關(guan)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)450nm 波長(chang)(chang)下測(ce)(ce)(ce)(ce)(ce)定吸(xi)(xi)光(guang)(guang)度(du)(du)(du)(du)(du)(du)（OD 值(zhi)(zhi)(zhi)），計(ji)(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)。保(bao)(bao)(bao)(bao)存(cun)(cun)條(tiao)件及(ji)(ji)有(you)(you)效(xiao)期(qi)(qi)本(ben)(ben)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)體(ti)(ti)外定量(liang)(liang)(liang)檢(jian)測(ce)(ce)(ce)(ce)(ce)血(xue)(xue)清(qing)、血(xue)(xue)漿(jiang)、組織勻(yun)(yun)漿(jiang)及(ji)(ji)相(xiang)(xiang)關(guan)液(ye)(ye)(ye)體(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)中(zhong)(zhong)脂肪酸結(jie)合(he)(he)蛋(dan)白(bai)(bai)1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)(liang)。有(you)(you)效(xiao)期(qi)(qi)：6個月(yue)(yue)保(bao)(bao)(bao)(bao)存(cun)(cun)條(tiao)件：2-8℃小(xiao)鼠(shu)胰島(dao)素(su)(su)(su)(su)(su)樣(yang)(yang)(yang)(yang)(yang)生(sheng)長(chang)(chang)因子(zi) 1（IGF-1）一(yi)(yi)(yi)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)(cai)集(ji)及(ji)(ji)保(bao)(bao)(bao)(bao)存(cun)(cun) 1.血(xue)(xue)清(qing)：全血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)請(qing)(qing)(qing)(qing)于(yu)(yu)(yu)(yu)室溫(wen)放(fang)置(zhi)2小(xiao)時(shi)(shi)(shi)或(huo)(huo)(huo)(huo)4℃過(guo)(guo)(guo)(guo)一(yi)(yi)(yi)晚后(hou)(hou)于(yu)(yu)(yu)(yu)1000 x g離(li)(li)心20分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，取(qu)上(shang)(shang)清(qing)即(ji)可(ke)檢(jian)測(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)放(fang)于(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)，但(dan)應(ying)(ying)(ying)避(bi)免(mian)反(fan)(fan)(fan)復(fu)(fu)(fu)凍(dong)融。 2.血(xue)(xue)漿(jiang)：可(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)肝素(su)(su)(su)(su)(su)作為(wei)抗(kang)(kang)凝劑(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)采(cai)(cai)集(ji)后(hou)(hou)30分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)于(yu)(yu)(yu)(yu)2 - 8° C 1000 x g離(li)(li)心15分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)放(fang)于(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)，但(dan)應(ying)(ying)(ying)避(bi)免(mian)反(fan)(fan)(fan)復(fu)(fu)(fu)凍(dong)融。 3.細(xi)胞培養物(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)清(qing)或(huo)(huo)(huo)(huo)其(qi)(qi)(qi)它(ta)生(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)：1000 x g離(li)(li)心20分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，取(qu)上(shang)(shang)清(qing)即(ji)可(ke)檢(jian)測(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)放(fang)于(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)，但(dan)應(ying)(ying)(ying)避(bi)免(mian)反(fan)(fan)(fan)復(fu)(fu)(fu)凍(dong)融。 注：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)溶(rong)(rong)血(xue)(xue)會影響檢(jian)測(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)，因此(ci)溶(rong)(rong)血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)不(bu)(bu)(bu)(bu)宜進(jin)(jin)(jin)行此(ci)項檢(jian)測(ce)(ce)(ce)(ce)(ce)。二、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)原則(ze)   首先(xian)(xian)通過(guo)(guo)(guo)(guo)文獻檢(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)式(shi)了解待(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含量(liang)(liang)(liang)，確定適當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)倍數(shu)(shu)。只有(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍(wei)內(nei)(nei)(nei)(nei)(nei)，檢(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果(guo)才是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)，應(ying)(ying)(ying)做(zuo)(zuo)好(hao)詳細(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)(ji)錄。計(ji)(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)時(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)了“N”倍，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)應(ying)(ying)(ying)再乘(cheng)以(yi)(yi)(yi)(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)原則(ze)：2瓶(ping)，每(mei)(mei)(mei)(mei)瓶(ping)臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)至1ml，蓋(gai)好(hao)后(hou)(hou)靜(jing)置(zhi)10分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)上(shang)(shang)，然后(hou)(hou)反(fan)(fan)(fan)復(fu)(fu)(fu)顛(dian)倒/搓動以(yi)(yi)(yi)(yi)助溶(rong)(rong)解，其(qi)(qi)(qi)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)300 pg/ml，做(zuo)(zuo)系列倍比(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)后(hou)(hou)，分(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)直(zhi)(zhi)接作為(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)15分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。 如(ru)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)：取(qu)0.5ml（不(bu)(bu)(bu)(bu)要少(shao)于(yu)(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)含0.5ml樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中(zhong)(zhong)，混(hun)(hun)(hun)勻(yun)(yun)即(ji)可(ke)，其(qi)(qi)(qi)余濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)以(yi)(yi)(yi)(yi)此(ci)類(lei)推。2.生(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)原則(ze)： 臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)生(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)前(qian)(qian)根(gen)據(ju)預先(xian)(xian)計(ji)(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)實(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)所(suo)需(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)100μl），實(shi)(shi)際配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)10μl生(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl生(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)(hun)(hun)勻(yun)(yun)，在(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)(yi)(yi)小(xiao)時(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。3.辣(la)根(gen)過(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)原則(ze)： 臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)辣(la)根(gen)過(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)前(qian)(qian)根(gen)據(ju)預先(xian)(xian)計(ji)(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)實(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)所(suo)需(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)100μl），實(shi)(shi)際配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)10μl辣(la)根(gen)過(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl辣(la)根(gen)過(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)(hun)(hun)勻(yun)(yun)，在(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)(yi)(yi)小(xiao)時(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。 操(cao)作步驟 實(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)開始前(qian)(qian)，請(qing)(qing)(qing)(qing)提(ti)前(qian)(qian)配(pei)(pei)(pei)(pei)置(zhi)好(hao)所(suo)有(you)(you)試(shi)(shi)劑(ji)(ji)(ji)，試(shi)(shi)劑(ji)(ji)(ji)或(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)時(shi)(shi)(shi)，均需(xu)(xu)(xu)混(hun)(hun)(hun)勻(yun)(yun)，混(hun)(hun)(hun)勻(yun)(yun)時(shi)(shi)(shi)盡量(liang)(liang)(liang)避(bi)免(mian)起(qi)泡(pao)。每(mei)(mei)(mei)(mei)次(ci)(ci)檢(jian)測(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)該做(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)。如(ru)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)過(guo)(guo)(guo)(guo)高(gao)時(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)進(jin)(jin)(jin)行稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)，以(yi)(yi)(yi)(yi)使(shi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)符合(he)(he)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)測(ce)(ce)(ce)(ce)(ce)范圍(wei)。 小(xiao)鼠(shu)胰島(dao)素(su)(su)(su)(su)(su)樣(yang)(yang)(yang)(yang)(yang)生(sheng)長(chang)(chang)因子(zi) 1（IGF-1）三(san)、操(cao)作步驟1.加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)別(bie)設(she)空(kong)白(bai)(bai)孔(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)、待(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)。空(kong)白(bai)(bai)孔(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)100μl，余孔(kong)(kong)分(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)待(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)100μl，注意(yi)不(bu)(bu)(bu)(bu)要有(you)(you)氣泡(pao)，加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)(yu)(yu)(yu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)孔(kong)(kong)底(di)(di)部，盡量(liang)(liang)(liang)不(bu)(bu)(bu)(bu)觸及(ji)(ji)孔(kong)(kong)壁，輕(qing)輕(qing)晃(huang)動混(hun)(hun)(hun)勻(yun)(yun)，酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)蓋(gai)或(huo)(huo)(huo)(huo)覆膜，37℃反(fan)(fan)(fan)應(ying)(ying)(ying)120分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。 為(wei)保(bao)(bao)(bao)(bao)證(zheng)實(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)結(jie)果(guo)有(you)(you)效(xiao)性(xing)(xing)，每(mei)(mei)(mei)(mei)次(ci)(ci)實(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)請(qing)(qing)(qing)(qing)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)液(ye)(ye)(ye)。2.棄去(qu)(qu)液(ye)(ye)(ye)體(ti)(ti)，甩(shuai)(shuai)干，不(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)滌(di)。每(mei)(mei)(mei)(mei)孔(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)生(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)工(gong)(gong)(gong)作液(ye)(ye)(ye) 100μl（取(qu)1μl生(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)99μl生(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)(hun)(hun)勻(yun)(yun)，在(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)(yi)(yi)小(xiao)時(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。 3.溫(wen)育60分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)，棄去(qu)(qu)孔(kong)(kong)內(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)體(ti)(ti)，甩(shuai)(shuai)干，洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)3次(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)浸(jin)泡(pao)1-2分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)，甩(shuai)(shuai)干。 4.每(mei)(mei)(mei)(mei)孔(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)辣(la)根(gen)過(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)工(gong)(gong)(gong)作液(ye)(ye)(ye)（同(tong)生(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)工(gong)(gong)(gong)作液(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。 5.溫(wen)育60分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)，棄去(qu)(qu)孔(kong)(kong)內(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)體(ti)(ti)，甩(shuai)(shuai)干，洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)5次(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)浸(jin)泡(pao)1-2分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)，甩(shuai)(shuai)干。 6.依(yi)序每(mei)(mei)(mei)(mei)孔(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)90μl，37℃避(bi)光(guang)(guang)顯(xian)色(se)（30分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)，此(ci)時(shi)(shi)(shi)肉(rou)眼可(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)3-4孔(kong)(kong)有(you)(you)明顯(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯(ti)度(du)(du)(du)(du)(du)(du)藍色(se)，后(hou)(hou)3-4孔(kong)(kong)梯(ti)度(du)(du)(du)(du)(du)(du)不(bu)(bu)(bu)(bu)明顯(xian)，即(ji)可(ke)終(zhong)止(zhi)(zhi)）。 7.依(yi)序每(mei)(mei)(mei)(mei)孔(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)(zhi)溶(rong)(rong)液(ye)(ye)(ye)50μl，終(zhong)止(zhi)(zhi)反(fan)(fan)(fan)應(ying)(ying)(ying)（此(ci)時(shi)(shi)(shi)藍色(se)立(li)轉(zhuan)(zhuan)黃(huang)色(se)）。終(zhong)止(zhi)(zhi)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)順(shun)序應(ying)(ying)(ying)盡量(liang)(liang)(liang)與(yu)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)順(shun)序相(xiang)(xiang)同(tong)。為(wei)了保(bao)(bao)(bao)(bao)證(zheng)實(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)，底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)反(fan)(fan)(fan)應(ying)(ying)(ying)時(shi)(shi)(shi)間到(dao)后(hou)(hou)應(ying)(ying)(ying)盡快(kuai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)終(zhong)止(zhi)(zhi)液(ye)(ye)(ye)。 8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯儀在(zai)(zai)450nm波長(chang)(chang)依(yi)序測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)各(ge)孔(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)(guang)密度(du)(du)(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)）。 在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)(zhi)液(ye)(ye)(ye)后(hou)(hou)15分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)內(nei)(nei)(nei)(nei)(nei)進(jin)(jin)(jin)行檢(jian)測(ce)(ce)(ce)(ce)(ce)。 四、試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)小(xiao)技(ji)巧1. 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)初次(ci)(ci)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)時(shi)(shi)(shi)，應(ying)(ying)(ying)將(jiang)各(ge)種(zhong)試(shi)(shi)劑(ji)(ji)(ji)管離(li)(li)心數(shu)(shu)分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)便試(shi)(shi)劑(ji)(ji)(ji)集(ji)中(zhong)(zhong)到(dao)管底(di)(di)。2. 每(mei)(mei)(mei)(mei)次(ci)(ci)實(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)留一(yi)(yi)(yi)孔(kong)(kong)作為(wei)空(kong)白(bai)(bai)調零孔(kong)(kong)，該孔(kong)(kong)不(bu)(bu)(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)任何試(shi)(shi)劑(ji)(ji)(ji)，只是**加(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)及(ji)(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)時(shi)(shi)(shi)先(xian)(xian)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)此(ci)孔(kong)(kong)調OD值(zhi)(zhi)(zhi)至零。 3. 為(wei)防止(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)時(shi)(shi)(shi)將(jiang)反(fan)(fan)(fan)應(ying)(ying)(ying)板(ban)(ban)(ban)(ban)(ban)放(fang)于(yu)(yu)(yu)(yu)鋪(pu)有(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉(bi)盒(he)(he)(he)內(nei)(nei)(nei)(nei)(nei)，酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)蓋(gai)或(huo)(huo)(huo)(huo)覆膜。 4. 未使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)或(huo)(huo)(huo)(huo)者試(shi)(shi)劑(ji)(ji)(ji)，請(qing)(qing)(qing)(qing)于(yu)(yu)(yu)(yu)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、生(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)工(gong)(gong)(gong)作液(ye)(ye)(ye)、辣(la)根(gen)過(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)工(gong)(gong)(gong)作液(ye)(ye)(ye)請(qing)(qing)(qing)(qing)依(yi)據(ju)所(suo)需(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)置(zhi)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)(qing)(qing)勿重(zhong)復(fu)(fu)(fu)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)過(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、生(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)工(gong)(gong)(gong)作液(ye)(ye)(ye)或(huo)(huo)(huo)(huo)、辣(la)根(gen)過(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)工(gong)(gong)(gong)作液(ye)(ye)(ye)。 5. 建議檢(jian)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)均設(she)雙孔(kong)(kong)測(ce)(ce)(ce)(ce)(ce)定，以(yi)(yi)(yi)(yi)保(bao)(bao)(bao)(bao)證(zheng)檢(jian)測(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)。 洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)方(fang)(fang)法(fa) 手工(gong)(gong)(gong)洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)方(fang)(fang)法(fa)：吸(xi)(xi)去(qu)(qu)（不(bu)(bu)(bu)(bu)可(ke)觸及(ji)(ji)板(ban)(ban)(ban)(ban)(ban)壁）或(huo)(huo)(huo)(huo)甩(shuai)(shuai)掉酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)內(nei)(nei)(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)體(ti)(ti)；在(zai)(zai)實(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)臺上(shang)(shang)鋪(pu)墊(dian)幾層(ceng)吸(xi)(xi)水紙(zhi)，酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)朝下用(yong)(yong)(yong)(yong)(yong)(yong)(yong)力拍幾次(ci)(ci)；將(jiang)推薦(jian)(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)緩沖(chong)液(ye)(ye)(ye)至少(shao)0.3ml注入(ru)孔(kong)(kong)內(nei)(nei)(nei)(nei)(nei)，浸(jin)泡(pao)1-2分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。根(gen)據(ju)需(xu)(xu)(xu)要，重(zhong)復(fu)(fu)(fu)此(ci)過(guo)(guo)(guo)(guo)程(cheng)數(shu)(shu)次(ci)(ci)。 自動洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)：如(ru)果(guo)有(you)(you)自動洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)機，應(ying)(ying)(ying)在(zai)(zai)熟練使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)再用(yong)(yong)(yong)(yong)(yong)(yong)(yong)到(dao)正式(shi)實(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)過(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)。 計(ji)(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan) 以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)為(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)半對數(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)繪出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)，根(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)查出相(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)；再乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)倍數(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)(zhi)線(xian)(xian)回(hui)歸(gui)方(fang)(fang)程(cheng)式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代(dai)入(ru)方(fang)(fang)程(cheng)式(shi)，計(ji)(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)出樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)，再乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)倍數(shu)(shu)，即(ji)為(wei)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)。 五、技(ji)術小(xiao)提(ti)示(shi)1.當(dang)混(hun)(hun)(hun)合(he)(he)蛋(dan)白(bai)(bai)溶(rong)(rong)液(ye)(ye)(ye)時(shi)(shi)(shi)應(ying)(ying)(ying)盡量(liang)(liang)(liang)輕(qing)緩，避(bi)免(mian)起(qi)泡(pao)。2. 洗(xi)(xi)滌(di)過(guo)(guo)(guo)(guo)程(cheng)非(fei)常重(zhong)要，不(bu)(bu)(bu)(bu)充分(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)易造(zao)成假陽性(xing)(xing)。 3. 一(yi)(yi)(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)間控制(zhi)(zhi)(zhi)(zhi)(zhi)在(zai)(zai)5分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)數(shu)(shu)量(liang)(liang)(liang)多，推薦(jian)(jian)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)排槍(qiang)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)。 4. 請(qing)(qing)(qing)(qing)每(mei)(mei)(mei)(mei)次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)定的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi)(shi)做(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)，做(zuo)(zuo)復(fu)(fu)(fu)孔(kong)(kong)。 5. 如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)中(zhong)(zhong)待(dai)測(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)(liang)(liang)過(guo)(guo)(guo)(guo)高(gao)，請(qing)(qing)(qing)(qing)先(xian)(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)后(hou)(hou)再測(ce)(ce)(ce)(ce)(ce)定，計(ji)(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)時(shi)(shi)(shi)請(qing)(qing)(qing)(qing)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)倍數(shu)(shu)。 6. 在(zai)(zai)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、檢(jian)測(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)液(ye)(ye)(ye)工(gong)(gong)(gong)作液(ye)(ye)(ye)時(shi)(shi)(shi)，請(qing)(qing)(qing)(qing)以(yi)(yi)(yi)(yi)相(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，不(bu)(bu)(bu)(bu)能混(hun)(hun)(hun)淆。 7. 底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)(qing)(qing)避(bi)光(guang)(guang)保(bao)(bao)(bao)(bao)存(cun)(cun)。 8. 不(bu)(bu)(bu)(bu)要用(yong)(yong)(yong)(yong)(yong)(yong)(yong)其(qi)(qi)(qi)它(ta)生(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)劑(ji)(ji)(ji)替換試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)劑(ji)(ji)(ji)。 六、注意(yi)事項1.收(shou)集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)前(qian)(qian)必須清(qing)楚要檢(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成份是否足夠穩定。對收(shou)集(ji)后(hou)(hou)當(dang)天進(jin)(jin)(jin)行檢(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)，儲(chu)存(cun)(cun)在(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)有(you)(you)特殊原因需(xu)(xu)(xu)要周期(qi)(qi)收(shou)集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)， 將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)及(ji)(ji)時(shi)(shi)(shi)分(fen)(fen)裝(zhuang)后(hou)(hou)放(fang)在(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)-70 ℃條(tiao)件下保(bao)(bao)(bao)(bao)存(cun)(cun)。避(bi)免(mian)反(fan)(fan)(fan)復(fu)(fu)(fu)凍(dong)融。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)2 -8 ℃可(ke)保(bao)(bao)(bao)(bao)存(cun)(cun)48 小(xiao)時(shi)(shi)(shi)，-20 ℃可(ke)保(bao)(bao)(bao)(bao)存(cun)(cun)1 個月(yue)(yue)。-70度(du)(du)(du)(du)(du)(du)可(ke)保(bao)(bao)(bao)(bao)存(cun)(cun)6 個月(yue)(yue)。3.部分(fen)(fen)激素(su)(su)(su)(su)(su)類(lei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)需(xu)(xu)(xu)添加(jia)(jia)(jia)(jia)(jia)(jia)(jia)抑肽酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)。 4.計(ji)(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)方(fang)(fang)法(fa)：以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)繪出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)，根(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)查出相(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)；再乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)倍數(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)(zhi)線(xian)(xian)回(hui)歸(gui)方(fang)(fang)程(cheng)式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代(dai)入(ru)方(fang)(fang)程(cheng)式(shi)，計(ji)(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)出樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)，再乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)倍數(shu)(shu)，即(ji)為(wei)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)。 七、試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)性(xing)(xing)能1.樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)線(xian)(xian)性(xing)(xing)回(hui)歸(gui)與(yu)預期(qi)(qi)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)相(xiang)(xiang)關(guan)系數(shu)(shu)R值(zhi)(zhi)(zhi)為(wei)0.92以(yi)(yi)(yi)(yi)上(shang)(shang)。2.批內(nei)(nei)(nei)(nei)(nei)與(yu)批見應(ying)(ying)(ying)分(fen)(fen)別(bie)小(xiao)于(yu)(yu)(yu)(yu)9%和(he)15% 八、保(bao)(bao)(bao)(bao)存(cun)(cun)條(tiao)件及(ji)(ji)有(you)(you)效(xiao)期(qi)(qi)1.試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)保(bao)(bao)(bao)(bao)存(cun)(cun)：2-8℃。 2．有(you)(you)效(xiao)期(qi)(qi)：6個月(yue)(yue)

 小鼠(shu)神經(jing)(jing)膠(jiao)(jiao)質纖維酸(suan)(suan)(suan)性(xing)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)（GFAP）實(shi)(shi)(shi)驗(yan)(yan)原(yuan)理   試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)采用(yong)(yong)(yong)(yong)(yong)(yong)雙抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)一步(bu)夾心法酶(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)疫吸附試(shi)(shi)(shi)(shi)驗(yan)(yan)（ELISA）。往預先包被(bei)脂(zhi)肪酸(suan)(suan)(suan)結(jie)合(he)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)1(FABP1)捕(bu)獲抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被(bei)微孔(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，依(yi)次(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)入(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)，經(jing)(jing)過(guo)(guo)(guo)(guo)溫育(yu)并(bing)徹底(di)(di)洗(xi)(xi)滌(di)。用(yong)(yong)(yong)(yong)(yong)(yong)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯色(se)(se)，TMB在(zai)(zai)(zai)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催(cui)化(hua)(hua)下(xia)轉(zhuan)化(hua)(hua)成(cheng)藍(lan)色(se)(se)，并(bing)在(zai)(zai)(zai)酸(suan)(suan)(suan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)下(xia)轉(zhuan)化(hua)(hua)成(cheng)＊終(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)色(se)(se)。顏色(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂(zhi)肪酸(suan)(suan)(suan)結(jie)合(he)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)1(FABP1)呈正相關。用(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)在(zai)(zai)(zai)450nm 波長下(xia)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定吸光(guang)(guang)度(du)(du)(du)(du)（OD 值(zhi)），計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)。保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)條(tiao)件(jian)及(ji)(ji)(ji)(ji)(ji)(ji)有(you)(you)(you)(you)效(xiao)期(qi)(qi)(qi)本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)體(ti)(ti)(ti)外定量(liang)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)血(xue)(xue)清(qing)、血(xue)(xue)漿、組(zu)織(zhi)勻(yun)漿及(ji)(ji)(ji)(ji)(ji)(ji)相關液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)脂(zhi)肪酸(suan)(suan)(suan)結(jie)合(he)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)量(liang)。有(you)(you)(you)(you)效(xiao)期(qi)(qi)(qi)：6個(ge)月保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)條(tiao)件(jian)：2-8℃小鼠(shu)神經(jing)(jing)膠(jiao)(jiao)質纖維酸(suan)(suan)(suan)性(xing)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)（GFAP）一、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采集(ji)及(ji)(ji)(ji)(ji)(ji)(ji)保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun) 1.血(xue)(xue)清(qing)：全(quan)血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)請(qing)(qing)(qing)于(yu)(yu)室溫放置(zhi)2小時(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)(huo)4℃過(guo)(guo)(guo)(guo)一晚后(hou)(hou)于(yu)(yu)1000 x g離(li)心20分(fen)(fen)(fen)(fen)(fen)(fen)鐘，取(qu)(qu)上(shang)清(qing)即(ji)(ji)可(ke)(ke)(ke)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放于(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)(mian)反(fan)(fan)復(fu)凍融(rong)。 2.血(xue)(xue)漿：可(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)肝素(su)(su)作(zuo)(zuo)(zuo)為抗(kang)(kang)(kang)(kang)(kang)凝(ning)劑(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)采集(ji)后(hou)(hou)30分(fen)(fen)(fen)(fen)(fen)(fen)鐘內(nei)(nei)(nei)(nei)于(yu)(yu)2 - 8° C 1000 x g離(li)心15分(fen)(fen)(fen)(fen)(fen)(fen)鐘，或(huo)(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放于(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)(mian)反(fan)(fan)復(fu)凍融(rong)。 3.細(xi)胞培養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)清(qing)或(huo)(huo)(huo)(huo)其(qi)(qi)它(ta)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)：1000 x g離(li)心20分(fen)(fen)(fen)(fen)(fen)(fen)鐘，取(qu)(qu)上(shang)清(qing)即(ji)(ji)可(ke)(ke)(ke)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放于(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)(mian)反(fan)(fan)復(fu)凍融(rong)。 注：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)溶(rong)(rong)血(xue)(xue)會影響(xiang)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)，因(yin)此(ci)(ci)溶(rong)(rong)血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)不(bu)(bu)(bu)宜進行(xing)此(ci)(ci)項檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。二、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)   首先通(tong)過(guo)(guo)(guo)(guo)文(wen)獻檢(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方式(shi)(shi)了解待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含(han)量(liang)，確(que)定適當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)。只有(you)(you)(you)(you)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍(wei)內(nei)(nei)(nei)(nei)，檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果(guo)才是準(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)做(zuo)(zuo)好詳(xiang)細(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)錄(lu)。計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)再乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：2瓶，每(mei)(mei)(mei)(mei)瓶臨用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至1ml，蓋好后(hou)(hou)靜置(zhi)10分(fen)(fen)(fen)(fen)(fen)(fen)鐘以(yi)(yi)(yi)(yi)(yi)上(shang)，然后(hou)(hou)反(fan)(fan)復(fu)顛倒/搓動(dong)以(yi)(yi)(yi)(yi)(yi)助溶(rong)(rong)解，其(qi)(qi)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)為300 pg/ml，做(zuo)(zuo)系列倍(bei)(bei)比稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)，分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)直接(jie)作(zuo)(zuo)(zuo)為標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 如(ru)(ru)(ru)(ru)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取(qu)(qu)0.5ml（不(bu)(bu)(bu)要少(shao)于(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)入(ru)含(han)0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)(guan)中(zhong)(zhong)(zhong)，混(hun)勻(yun)即(ji)(ji)可(ke)(ke)(ke)，其(qi)(qi)余濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)以(yi)(yi)(yi)(yi)(yi)此(ci)(ci)類(lei)推。2.生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)： 臨用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)(gen)(gen)據(ju)預先計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)所需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)際配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)多配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)(ru)10μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕輕混(hun)勻(yun)，在(zai)(zai)(zai)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一小時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。3.辣根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和(he)(he)(he)(he)素(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)： 臨用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)辣根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和(he)(he)(he)(he)素(su)(su)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)(gen)(gen)據(ju)預先計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)所需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)際配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)多配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)(ru)10μl辣根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和(he)(he)(he)(he)素(su)(su)加(jia)(jia)(jia)(jia)(jia)990μl辣根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和(he)(he)(he)(he)素(su)(su)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕輕混(hun)勻(yun)，在(zai)(zai)(zai)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一小時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 操作(zuo)(zuo)(zuo)步(bu)驟(zou) 實(shi)(shi)(shi)驗(yan)(yan)開(kai)始前(qian)(qian)(qian)，請(qing)(qing)(qing)提(ti)前(qian)(qian)(qian)配(pei)(pei)(pei)(pei)(pei)(pei)置(zhi)好所有(you)(you)(you)(you)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)或(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)，均(jun)需(xu)(xu)混(hun)勻(yun)，混(hun)勻(yun)時(shi)(shi)(shi)(shi)(shi)盡量(liang)避(bi)(bi)免(mian)(mian)(mian)起泡(pao)(pao)。每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)(ying)(ying)(ying)該做(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)線(xian)(xian)。如(ru)(ru)(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)過(guo)(guo)(guo)(guo)高時(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)進行(xing)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)(yi)使(shi)(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合(he)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范圍(wei)。 小鼠(shu)神經(jing)(jing)膠(jiao)(jiao)質纖維酸(suan)(suan)(suan)性(xing)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)（GFAP）三、操作(zuo)(zuo)(zuo)步(bu)驟(zou)1.加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)設空(kong)(kong)白(bai)(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)、待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)。空(kong)(kong)白(bai)(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注意不(bu)(bu)(bu)要有(you)(you)(you)(you)氣泡(pao)(pao)，加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)于(yu)(yu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)底(di)(di)部(bu)，盡量(liang)不(bu)(bu)(bu)觸及(ji)(ji)(ji)(ji)(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)壁，輕輕晃(huang)動(dong)混(hun)勻(yun)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)上(shang)蓋或(huo)(huo)(huo)(huo)覆(fu)膜，37℃反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)(fen)鐘。 為保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)證(zheng)實(shi)(shi)(shi)驗(yan)(yan)結(jie)果(guo)有(you)(you)(you)(you)效(xiao)性(xing)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)請(qing)(qing)(qing)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)。2.棄去(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干(gan)(gan)(gan)，不(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)滌(di)。每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取(qu)(qu)1μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕輕混(hun)勻(yun)，在(zai)(zai)(zai)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一小時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)(fen)鐘。 3.溫育(yu)60分(fen)(fen)(fen)(fen)(fen)(fen)鐘后(hou)(hou)，棄去(qu)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干(gan)(gan)(gan)，洗(xi)(xi)板(ban)(ban)(ban)3次(ci)(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)浸泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)干(gan)(gan)(gan)。 4.每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)辣根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和(he)(he)(he)(he)素(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同(tong)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)(fen)鐘。 5.溫育(yu)60分(fen)(fen)(fen)(fen)(fen)(fen)鐘后(hou)(hou)，棄去(qu)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干(gan)(gan)(gan)，洗(xi)(xi)板(ban)(ban)(ban)5次(ci)(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)浸泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)干(gan)(gan)(gan)。 6.依(yi)序每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)(bi)光(guang)(guang)顯色(se)(se)（30分(fen)(fen)(fen)(fen)(fen)(fen)鐘內(nei)(nei)(nei)(nei)，此(ci)(ci)時(shi)(shi)(shi)(shi)(shi)肉眼可(ke)(ke)(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)有(you)(you)(you)(you)明顯的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯(ti)度(du)(du)(du)(du)藍(lan)色(se)(se)，后(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)梯(ti)度(du)(du)(du)(du)不(bu)(bu)(bu)明顯，即(ji)(ji)可(ke)(ke)(ke)終(zhong)(zhong)止(zhi)）。 7.依(yi)序每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)止(zhi)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)止(zhi)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)（此(ci)(ci)時(shi)(shi)(shi)(shi)(shi)藍(lan)色(se)(se)立轉(zhuan)黃(huang)色(se)(se)）。終(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)入(ru)順序應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡量(liang)與底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)入(ru)順序相同(tong)。為了保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)證(zheng)實(shi)(shi)(shi)驗(yan)(yan)結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)確(que)性(xing)，底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)間(jian)到后(hou)(hou)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡快加(jia)(jia)(jia)(jia)(jia)入(ru)終(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 8.用(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)聯(lian)儀(yi)在(zai)(zai)(zai)450nm波長依(yi)序測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)各孔(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)(guang)密度(du)(du)(du)(du)（OD值(zhi)）。 在(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘以(yi)(yi)(yi)(yi)(yi)內(nei)(nei)(nei)(nei)進行(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。 四(si)、試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)小技巧1. 用(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)(zai)初(chu)次(ci)(ci)(ci)(ci)(ci)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)時(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)將(jiang)(jiang)各種試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)管(guan)(guan)離(li)心數(shu)(shu)分(fen)(fen)(fen)(fen)(fen)(fen)鐘，以(yi)(yi)(yi)(yi)(yi)便(bian)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)集(ji)中(zhong)(zhong)(zhong)到管(guan)(guan)底(di)(di)。2. 每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)留一孔(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)(zuo)為空(kong)(kong)白(bai)(bai)(bai)調(diao)零(ling)孔(kong)(kong)(kong)(kong)(kong)，該孔(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)加(jia)(jia)(jia)(jia)(jia)任何(he)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)，只是**加(jia)(jia)(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)及(ji)(ji)(ji)(ji)(ji)(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)時(shi)(shi)(shi)(shi)(shi)先用(yong)(yong)(yong)(yong)(yong)(yong)此(ci)(ci)孔(kong)(kong)(kong)(kong)(kong)調(diao)OD值(zhi)至零(ling)。 3. 為防止(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)(shi)驗(yan)(yan)時(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)板(ban)(ban)(ban)放于(yu)(yu)鋪(pu)有(you)(you)(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)(he)內(nei)(nei)(nei)(nei)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)上(shang)蓋或(huo)(huo)(huo)(huo)覆(fu)膜。 4. 未使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)或(huo)(huo)(huo)(huo)者試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)，請(qing)(qing)(qing)于(yu)(yu)2-8℃保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和(he)(he)(he)(he)素(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)請(qing)(qing)(qing)依(yi)據(ju)所需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)配(pei)(pei)(pei)(pei)(pei)(pei)置(zhi)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)(qing)勿重(zhong)復(fu)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)已(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)、辣根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和(he)(he)(he)(he)素(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5. 建(jian)議檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)均(jun)設雙孔(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定，以(yi)(yi)(yi)(yi)(yi)保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)證(zheng)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)確(que)性(xing)。 洗(xi)(xi)板(ban)(ban)(ban)方法 手工(gong)(gong)(gong)洗(xi)(xi)板(ban)(ban)(ban)方法：吸去(qu)（不(bu)(bu)(bu)可(ke)(ke)(ke)觸及(ji)(ji)(ji)(ji)(ji)(ji)板(ban)(ban)(ban)壁）或(huo)(huo)(huo)(huo)甩(shuai)掉酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)內(nei)(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)；在(zai)(zai)(zai)實(shi)(shi)(shi)驗(yan)(yan)臺上(shang)鋪(pu)墊幾(ji)層吸水紙(zhi)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)朝下(xia)用(yong)(yong)(yong)(yong)(yong)(yong)力拍(pai)幾(ji)次(ci)(ci)(ci)(ci)(ci)；將(jiang)(jiang)推薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)緩(huan)沖液(ye)(ye)(ye)(ye)(ye)(ye)(ye)至少(shao)0.3ml注入(ru)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)，浸泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘。根(gen)(gen)(gen)據(ju)需(xu)(xu)要，重(zhong)復(fu)此(ci)(ci)過(guo)(guo)(guo)(guo)程(cheng)數(shu)(shu)次(ci)(ci)(ci)(ci)(ci)。 自(zi)動(dong)洗(xi)(xi)板(ban)(ban)(ban)：如(ru)(ru)(ru)(ru)果(guo)有(you)(you)(you)(you)自(zi)動(dong)洗(xi)(xi)板(ban)(ban)(ban)機，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)在(zai)(zai)(zai)熟(shu)練使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)再用(yong)(yong)(yong)(yong)(yong)(yong)到正式(shi)(shi)實(shi)(shi)(shi)驗(yan)(yan)過(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)。 計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan) 以(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)為橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)為縱(zong)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通(tong)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)半對數(shu)(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)線(xian)(xian)，根(gen)(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)線(xian)(xian)查出(chu)相應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)；再乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)與OD值(zhi)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)回歸(gui)方程(cheng)式(shi)(shi)，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代入(ru)方程(cheng)式(shi)(shi)，計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)，再乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)，即(ji)(ji)為樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)。 五、技術小提(ti)示1.當(dang)混(hun)合(he)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡量(liang)輕緩(huan)，避(bi)(bi)免(mian)(mian)(mian)起泡(pao)(pao)。2. 洗(xi)(xi)滌(di)過(guo)(guo)(guo)(guo)程(cheng)非常重(zhong)要，不(bu)(bu)(bu)充分(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)易造成(cheng)假陽(yang)性(xing)。 3. 一次(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)間(jian)控制(zhi)(zhi)(zhi)在(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)鐘內(nei)(nei)(nei)(nei)，如(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)(shu)量(liang)多，推薦使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。 4. 請(qing)(qing)(qing)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi)(shi)(shi)(shi)做(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)線(xian)(xian)，做(zuo)(zuo)復(fu)孔(kong)(kong)(kong)(kong)(kong)。 5. 如(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含(han)量(liang)過(guo)(guo)(guo)(guo)高，請(qing)(qing)(qing)先稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)再測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定，計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)時(shi)(shi)(shi)(shi)(shi)請(qing)(qing)(qing)乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)。 6. 在(zai)(zai)(zai)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)，請(qing)(qing)(qing)以(yi)(yi)(yi)(yi)(yi)相應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，不(bu)(bu)(bu)能混(hun)淆。 7. 底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)(qing)避(bi)(bi)光(guang)(guang)保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。 8. 不(bu)(bu)(bu)要用(yong)(yong)(yong)(yong)(yong)(yong)其(qi)(qi)它(ta)生(sheng)(sheng)產廠家(jia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)替(ti)換試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)。 六、注意事項1.收(shou)集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)前(qian)(qian)(qian)必須清(qing)楚要檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)份(fen)是否足夠穩定。對收(shou)集(ji)后(hou)(hou)當(dang)天進行(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)，儲存(cun)(cun)(cun)在(zai)(zai)(zai)4 ℃備(bei)用(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)(ru)(ru)有(you)(you)(you)(you)特(te)殊原(yuan)因(yin)需(xu)(xu)要周(zhou)期(qi)(qi)(qi)收(shou)集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)， 將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)及(ji)(ji)(ji)(ji)(ji)(ji)時(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)(fen)裝后(hou)(hou)放在(zai)(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)-70 ℃條(tiao)件(jian)下(xia)保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。避(bi)(bi)免(mian)(mian)(mian)反(fan)(fan)復(fu)凍融(rong)。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)48 小時(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)1 個(ge)月。-70度(du)(du)(du)(du)可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)6 個(ge)月。3.部(bu)分(fen)(fen)(fen)(fen)(fen)(fen)激素(su)(su)類(lei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)需(xu)(xu)添(tian)加(jia)(jia)(jia)(jia)(jia)抑肽酶(mei)(mei)(mei)。 4.計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)方法：以(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)為橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)為縱(zong)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)線(xian)(xian)，根(gen)(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)線(xian)(xian)查出(chu)相應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)；再乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)與OD值(zhi)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)回歸(gui)方程(cheng)式(shi)(shi)，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代入(ru)方程(cheng)式(shi)(shi)，計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)，再乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)，即(ji)(ji)為樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)。 七、試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)性(xing)能1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)性(xing)回歸(gui)與預期(qi)(qi)(qi)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)相關系數(shu)(shu)R值(zhi)為0.92以(yi)(yi)(yi)(yi)(yi)上(shang)。2.批內(nei)(nei)(nei)(nei)與批見應(ying)(ying)(ying)(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)小于(yu)(yu)9%和(he)(he)(he)(he)15% 八、保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)條(tiao)件(jian)及(ji)(ji)(ji)(ji)(ji)(ji)有(you)(you)(you)(you)效(xiao)期(qi)(qi)(qi)1.試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)：2-8℃。 2．有(you)(you)(you)(you)效(xiao)期(qi)(qi)(qi)：6個(ge)月

 小(xiao)(xiao)鼠(shu)(shu)總(zong) Tau 蛋(dan)(dan)(dan)(dan)白(bai)（T-Tau）ELISA 檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)實(shi)驗(yan)原(yuan)(yuan)(yuan)理   試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙(shuang)抗(kang)(kang)體(ti)(ti)(ti)(ti)一(yi)(yi)步(bu)夾心(xin)法酶(mei)(mei)(mei)(mei)聯免(mian)(mian)疫吸附試(shi)(shi)(shi)驗(yan)（ELISA）。往預(yu)先包(bao)(bao)被脂肪(fang)(fang)酸(suan)結(jie)合(he)蛋(dan)(dan)(dan)(dan)白(bai)1(FABP1)捕獲抗(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包(bao)(bao)被微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，依(yi)次(ci)(ci)(ci)(ci)加(jia)(jia)入(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)體(ti)(ti)(ti)(ti)，經(jing)過(guo)溫(wen)育(yu)(yu)并徹底(di)洗(xi)(xi)滌。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯色(se)，TMB在(zai)(zai)(zai)(zai)過(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催(cui)化(hua)(hua)(hua)下轉化(hua)(hua)(hua)成(cheng)藍色(se)，并在(zai)(zai)(zai)(zai)酸(suan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)下轉化(hua)(hua)(hua)成(cheng)＊終(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)。顏色(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂肪(fang)(fang)酸(suan)結(jie)合(he)蛋(dan)(dan)(dan)(dan)白(bai)1(FABP1)呈(cheng)正相(xiang)關(guan)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)(zai)(zai)450nm 波長下測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)吸光(guang)度(du)(du)(du)（OD 值(zhi)(zhi)(zhi)(zhi)），計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)。保(bao)(bao)(bao)(bao)(bao)存(cun)條(tiao)件(jian)(jian)及(ji)(ji)(ji)有(you)(you)效(xiao)(xiao)期(qi)本(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)體(ti)(ti)(ti)(ti)外定(ding)(ding)(ding)量(liang)(liang)(liang)(liang)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)血清(qing)、血漿(jiang)、組織勻(yun)(yun)漿(jiang)及(ji)(ji)(ji)相(xiang)關(guan)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)中(zhong)脂肪(fang)(fang)酸(suan)結(jie)合(he)蛋(dan)(dan)(dan)(dan)白(bai)1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)量(liang)(liang)(liang)(liang)。有(you)(you)效(xiao)(xiao)期(qi)：6個(ge)月保(bao)(bao)(bao)(bao)(bao)存(cun)條(tiao)件(jian)(jian)：2-8℃小(xiao)(xiao)鼠(shu)(shu)總(zong) Tau 蛋(dan)(dan)(dan)(dan)白(bai)（T-Tau）ELISA 檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)一(yi)(yi)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集及(ji)(ji)(ji)保(bao)(bao)(bao)(bao)(bao)存(cun) 1.血清(qing)：全血標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)請(qing)(qing)(qing)于(yu)(yu)(yu)(yu)室溫(wen)放(fang)置2小(xiao)(xiao)時(shi)(shi)或(huo)(huo)(huo)(huo)4℃過(guo)一(yi)(yi)晚后(hou)(hou)(hou)于(yu)(yu)(yu)(yu)1000 x g離(li)(li)心(xin)20分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，取上(shang)清(qing)即(ji)可(ke)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)存(cun)，但應(ying)(ying)(ying)避(bi)(bi)(bi)免(mian)(mian)反(fan)(fan)(fan)復(fu)(fu)(fu)凍融(rong)。 2.血漿(jiang)：可(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)肝素(su)(su)(su)(su)(su)(su)作(zuo)(zuo)為(wei)(wei)抗(kang)(kang)凝劑(ji)(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)采(cai)集后(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)于(yu)(yu)(yu)(yu)2 - 8° C 1000 x g離(li)(li)心(xin)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)存(cun)，但應(ying)(ying)(ying)避(bi)(bi)(bi)免(mian)(mian)反(fan)(fan)(fan)復(fu)(fu)(fu)凍融(rong)。 3.細胞(bao)培養(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)清(qing)或(huo)(huo)(huo)(huo)其(qi)(qi)它(ta)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)：1000 x g離(li)(li)心(xin)20分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，取上(shang)清(qing)即(ji)可(ke)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)存(cun)，但應(ying)(ying)(ying)避(bi)(bi)(bi)免(mian)(mian)反(fan)(fan)(fan)復(fu)(fu)(fu)凍融(rong)。 注(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)溶(rong)血會影響檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果，因此(ci)溶(rong)血標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)不(bu)宜進行此(ci)項檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。二、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)(yuan)則(ze)   首先通過(guo)文獻檢(jian)(jian)(jian)(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方式(shi)了解待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含(han)量(liang)(liang)(liang)(liang)，確(que)定(ding)(ding)(ding)適(shi)當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)。只有(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范(fan)圍內(nei)(nei)(nei)(nei)，檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果才是(shi)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)程(cheng)(cheng)(cheng)中(zhong)，應(ying)(ying)(ying)做(zuo)好詳細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)錄。計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)濃(nong)(nong)(nong)(nong)度(du)(du)(du)時(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)應(ying)(ying)(ying)再(zai)(zai)乘(cheng)(cheng)以(yi)(yi)(yi)(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)(yuan)則(ze)：2瓶(ping)，每(mei)(mei)(mei)(mei)瓶(ping)臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)(zhi)(zhi)1ml，蓋(gai)(gai)好后(hou)(hou)(hou)靜(jing)置10分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)上(shang)，然后(hou)(hou)(hou)反(fan)(fan)(fan)復(fu)(fu)(fu)顛(dian)倒/搓動(dong)(dong)以(yi)(yi)(yi)(yi)助溶(rong)解，其(qi)(qi)濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)300 pg/ml，做(zuo)系(xi)列倍(bei)比(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)直(zhi)接作(zuo)(zuo)為(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)度(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 如(ru)(ru)(ru)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取0.5ml（不(bu)要(yao)少(shao)于(yu)(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)入(ru)(ru)含(han)0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中(zhong)，混(hun)(hun)勻(yun)(yun)即(ji)可(ke)，其(qi)(qi)余濃(nong)(nong)(nong)(nong)度(du)(du)(du)以(yi)(yi)(yi)(yi)此(ci)類(lei)推(tui)。2.生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)(yuan)則(ze)： 臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)(gen)據(ju)(ju)預(yu)先計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)驗(yan)所(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際(ji)(ji)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)10μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)990μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕輕混(hun)(hun)勻(yun)(yun)，在(zai)(zai)(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)(yi)小(xiao)(xiao)時(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)。3.辣(la)(la)根(gen)(gen)過(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)素(su)(su)(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)(yuan)則(ze)： 臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)辣(la)(la)根(gen)(gen)過(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)素(su)(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)(gen)據(ju)(ju)預(yu)先計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)驗(yan)所(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際(ji)(ji)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)10μl辣(la)(la)根(gen)(gen)過(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)素(su)(su)(su)(su)(su)(su)加(jia)(jia)990μl辣(la)(la)根(gen)(gen)過(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)素(su)(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕輕混(hun)(hun)勻(yun)(yun)，在(zai)(zai)(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)(yi)小(xiao)(xiao)時(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 操作(zuo)(zuo)步(bu)驟 實(shi)驗(yan)開始前(qian)(qian)(qian)，請(qing)(qing)(qing)提前(qian)(qian)(qian)配(pei)(pei)(pei)置好所(suo)有(you)(you)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)或(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)，均需(xu)混(hun)(hun)勻(yun)(yun)，混(hun)(hun)勻(yun)(yun)時(shi)(shi)盡量(liang)(liang)(liang)(liang)避(bi)(bi)(bi)免(mian)(mian)起泡(pao)。每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)都(dou)應(ying)(ying)(ying)該做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)。如(ru)(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)過(guo)高(gao)時(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)進行稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)使(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合(he)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)圍。 小(xiao)(xiao)鼠(shu)(shu)總(zong) Tau 蛋(dan)(dan)(dan)(dan)白(bai)（T-Tau）ELISA 檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)三、操作(zuo)(zuo)步(bu)驟1.加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)設空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)意不(bu)要(yao)有(you)(you)氣泡(pao)，加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)于(yu)(yu)(yu)(yu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底(di)部，盡量(liang)(liang)(liang)(liang)不(bu)觸及(ji)(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕輕晃動(dong)(dong)混(hun)(hun)勻(yun)(yun)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)加(jia)(jia)上(shang)蓋(gai)(gai)或(huo)(huo)(huo)(huo)覆膜，37℃反(fan)(fan)(fan)應(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。 為(wei)(wei)保(bao)(bao)(bao)(bao)(bao)證實(shi)驗(yan)結(jie)果有(you)(you)效(xiao)(xiao)性(xing)(xing)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)驗(yan)請(qing)(qing)(qing)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)液(ye)(ye)(ye)(ye)(ye)。2.棄去液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干，不(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)滌。每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)99μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕輕混(hun)(hun)勻(yun)(yun)，在(zai)(zai)(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)(yi)小(xiao)(xiao)時(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。 3.溫(wen)育(yu)(yu)60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)，棄去孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干，洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)3次(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)干。 4.每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)辣(la)(la)根(gen)(gen)過(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)素(su)(su)(su)(su)(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)（同(tong)(tong)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。 5.溫(wen)育(yu)(yu)60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)，棄去孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干，洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)5次(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)干。 6.依(yi)序(xu)(xu)(xu)每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)(bi)(bi)光(guang)顯色(se)（30分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)，此(ci)時(shi)(shi)肉(rou)眼可(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)有(you)(you)明顯的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)藍色(se)，后(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)梯度(du)(du)(du)不(bu)明顯，即(ji)可(ke)終(zhong)(zhong)(zhong)止(zhi)）。 7.依(yi)序(xu)(xu)(xu)每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)終(zhong)(zhong)(zhong)止(zhi)溶(rong)液(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)(zhong)止(zhi)反(fan)(fan)(fan)應(ying)(ying)(ying)（此(ci)時(shi)(shi)藍色(se)立(li)轉黃色(se)）。終(zhong)(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)入(ru)(ru)順序(xu)(xu)(xu)應(ying)(ying)(ying)盡量(liang)(liang)(liang)(liang)與(yu)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)入(ru)(ru)順序(xu)(xu)(xu)相(xiang)同(tong)(tong)。為(wei)(wei)了保(bao)(bao)(bao)(bao)(bao)證實(shi)驗(yan)結(jie)果的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)，底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)(fan)(fan)應(ying)(ying)(ying)時(shi)(shi)間到后(hou)(hou)(hou)應(ying)(ying)(ying)盡快(kuai)加(jia)(jia)入(ru)(ru)終(zhong)(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)。 8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)聯儀在(zai)(zai)(zai)(zai)450nm波長依(yi)序(xu)(xu)(xu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)(liang)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)密(mi)度(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)）。 在(zai)(zai)(zai)(zai)加(jia)(jia)終(zhong)(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)內(nei)(nei)(nei)(nei)進行檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。 四、試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)小(xiao)(xiao)技(ji)巧(qiao)1. 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)(zai)(zai)初次(ci)(ci)(ci)(ci)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)時(shi)(shi)，應(ying)(ying)(ying)將(jiang)(jiang)各種試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)管離(li)(li)心(xin)數(shu)(shu)分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)集中(zhong)到管底(di)。2. 每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)驗(yan)留一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)為(wei)(wei)空白(bai)調(diao)零(ling)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，該孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)任何試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，只是(shi)**加(jia)(jia)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)(ye)及(ji)(ji)(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)(liang)時(shi)(shi)先用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)此(ci)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)調(diao)OD值(zhi)(zhi)(zhi)(zhi)至(zhi)(zhi)(zhi)零(ling)。 3. 為(wei)(wei)防止(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)驗(yan)時(shi)(shi)將(jiang)(jiang)反(fan)(fan)(fan)應(ying)(ying)(ying)板(ban)(ban)(ban)(ban)(ban)放(fang)于(yu)(yu)(yu)(yu)鋪(pu)有(you)(you)濕布(bu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密(mi)閉盒(he)內(nei)(nei)(nei)(nei)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)加(jia)(jia)上(shang)蓋(gai)(gai)或(huo)(huo)(huo)(huo)覆膜。 4. 未使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)或(huo)(huo)(huo)(huo)者試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，請(qing)(qing)(qing)于(yu)(yu)(yu)(yu)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)、辣(la)(la)根(gen)(gen)過(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)素(su)(su)(su)(su)(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)請(qing)(qing)(qing)依(yi)據(ju)(ju)所(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)置使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)(qing)勿重(zhong)(zhong)復(fu)(fu)(fu)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)過(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)、辣(la)(la)根(gen)(gen)過(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)素(su)(su)(su)(su)(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)。 5. 建議檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)均設雙(shuang)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，以(yi)(yi)(yi)(yi)保(bao)(bao)(bao)(bao)(bao)證檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)。 洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)方法 手工(gong)(gong)(gong)洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)方法：吸去（不(bu)可(ke)觸及(ji)(ji)(ji)板(ban)(ban)(ban)(ban)(ban)壁）或(huo)(huo)(huo)(huo)甩(shuai)(shuai)掉酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)內(nei)(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)；在(zai)(zai)(zai)(zai)實(shi)驗(yan)臺上(shang)鋪(pu)墊幾(ji)層吸水(shui)紙，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)朝下用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)力拍幾(ji)次(ci)(ci)(ci)(ci)；將(jiang)(jiang)推(tui)薦(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌緩沖液(ye)(ye)(ye)(ye)(ye)至(zhi)(zhi)(zhi)少(shao)0.3ml注(zhu)入(ru)(ru)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)，浸泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。根(gen)(gen)據(ju)(ju)需(xu)要(yao)，重(zhong)(zhong)復(fu)(fu)(fu)此(ci)過(guo)程(cheng)(cheng)(cheng)數(shu)(shu)次(ci)(ci)(ci)(ci)。 自(zi)動(dong)(dong)洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)：如(ru)(ru)(ru)果有(you)(you)自(zi)動(dong)(dong)洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)機，應(ying)(ying)(ying)在(zai)(zai)(zai)(zai)熟(shu)練(lian)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)再(zai)(zai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)到正式(shi)實(shi)驗(yan)過(guo)程(cheng)(cheng)(cheng)中(zhong)。 計(ji)(ji)(ji)(ji)算(suan)(suan)(suan) 以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)(zhi)為(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)(zai)半(ban)對數(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)繪出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)，根(gen)(gen)據(ju)(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)查出(chu)(chu)相(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)；再(zai)(zai)乘(cheng)(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)(zhi)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)回歸(gui)方程(cheng)(cheng)(cheng)式(shi)，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)代入(ru)(ru)方程(cheng)(cheng)(cheng)式(shi)，計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)出(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)，再(zai)(zai)乘(cheng)(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)，即(ji)為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際(ji)(ji)濃(nong)(nong)(nong)(nong)度(du)(du)(du)。 五、技(ji)術小(xiao)(xiao)提示1.當混(hun)(hun)合(he)蛋(dan)(dan)(dan)(dan)白(bai)溶(rong)液(ye)(ye)(ye)(ye)(ye)時(shi)(shi)應(ying)(ying)(ying)盡量(liang)(liang)(liang)(liang)輕緩，避(bi)(bi)(bi)免(mian)(mian)起泡(pao)。2. 洗(xi)(xi)滌過(guo)程(cheng)(cheng)(cheng)非(fei)常(chang)重(zhong)(zhong)要(yao)，不(bu)充(chong)分(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌易造成(cheng)假陽(yang)性(xing)(xing)。 3. 一(yi)(yi)次(ci)(ci)(ci)(ci)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)間控制(zhi)(zhi)(zhi)在(zai)(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)，如(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)數(shu)(shu)量(liang)(liang)(liang)(liang)多，推(tui)薦(jian)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。 4. 請(qing)(qing)(qing)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)(tong)時(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)，做(zuo)復(fu)(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。 5. 如(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)中(zhong)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含(han)量(liang)(liang)(liang)(liang)過(guo)高(gao)，請(qing)(qing)(qing)先稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)再(zai)(zai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)時(shi)(shi)請(qing)(qing)(qing)乘(cheng)(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)。 6. 在(zai)(zai)(zai)(zai)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)液(ye)(ye)(ye)(ye)(ye)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)時(shi)(shi)，請(qing)(qing)(qing)以(yi)(yi)(yi)(yi)相(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)，不(bu)能(neng)混(hun)(hun)淆(xiao)。 7. 底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)(qing)避(bi)(bi)(bi)光(guang)保(bao)(bao)(bao)(bao)(bao)存(cun)。 8. 不(bu)要(yao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)其(qi)(qi)它(ta)生(sheng)(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)替換試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)。 六、注(zhu)意事項1.收(shou)集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)前(qian)(qian)(qian)必(bi)須(xu)清(qing)楚要(yao)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)份是(shi)否足夠穩定(ding)(ding)(ding)。對收(shou)集后(hou)(hou)(hou)當天進行檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)，儲存(cun)在(zai)(zai)(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)(ru)有(you)(you)特殊(shu)原(yuan)(yuan)(yuan)因需(xu)要(yao)周期(qi)收(shou)集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)， 將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)及(ji)(ji)(ji)時(shi)(shi)分(fen)(fen)(fen)(fen)(fen)(fen)裝后(hou)(hou)(hou)放(fang)在(zai)(zai)(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)-70 ℃條(tiao)件(jian)(jian)下保(bao)(bao)(bao)(bao)(bao)存(cun)。避(bi)(bi)(bi)免(mian)(mian)反(fan)(fan)(fan)復(fu)(fu)(fu)凍融(rong)。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)2 -8 ℃可(ke)保(bao)(bao)(bao)(bao)(bao)存(cun)48 小(xiao)(xiao)時(shi)(shi)，-20 ℃可(ke)保(bao)(bao)(bao)(bao)(bao)存(cun)1 個(ge)月。-70度(du)(du)(du)可(ke)保(bao)(bao)(bao)(bao)(bao)存(cun)6 個(ge)月。3.部分(fen)(fen)(fen)(fen)(fen)(fen)激素(su)(su)(su)(su)(su)(su)類(lei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)需(xu)添加(jia)(jia)抑肽酶(mei)(mei)(mei)(mei)。 4.計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)方法：以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)為(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)(zai)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)繪出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)，根(gen)(gen)據(ju)(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)查出(chu)(chu)相(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)；再(zai)(zai)乘(cheng)(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)(zhi)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)回歸(gui)方程(cheng)(cheng)(cheng)式(shi)，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)代入(ru)(ru)方程(cheng)(cheng)(cheng)式(shi)，計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)出(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)，再(zai)(zai)乘(cheng)(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)，即(ji)為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際(ji)(ji)濃(nong)(nong)(nong)(nong)度(du)(du)(du)。 七、試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)性(xing)(xing)能(neng)1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)性(xing)(xing)回歸(gui)與(yu)預(yu)期(qi)濃(nong)(nong)(nong)(nong)度(du)(du)(du)相(xiang)關(guan)系(xi)數(shu)(shu)R值(zhi)(zhi)(zhi)(zhi)為(wei)(wei)0.92以(yi)(yi)(yi)(yi)上(shang)。2.批內(nei)(nei)(nei)(nei)與(yu)批見應(ying)(ying)(ying)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)小(xiao)(xiao)于(yu)(yu)(yu)(yu)9%和(he)(he)15% 八、保(bao)(bao)(bao)(bao)(bao)存(cun)條(tiao)件(jian)(jian)及(ji)(ji)(ji)有(you)(you)效(xiao)(xiao)期(qi)1.試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)保(bao)(bao)(bao)(bao)(bao)存(cun)：2-8℃。 2．有(you)(you)效(xiao)(xiao)期(qi)：6個(ge)月

 小(xiao)(xiao)(xiao)鼠可溶(rong)(rong)性(xing)髓(sui)系(xi)細胞(bao)觸(chu)發受(shou)體(ti)(ti)(ti)-2（sTREM-2）ELISA 檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)劑(ji)盒(he)(he)(he)實(shi)驗原(yuan)理   試(shi)(shi)(shi)劑(ji)盒(he)(he)(he)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙抗(kang)體(ti)(ti)(ti)一步夾心(xin)法(fa)(fa)酶(mei)聯免(mian)(mian)疫吸(xi)附(fu)試(shi)(shi)(shi)驗（ELISA）。往(wang)預(yu)先包(bao)被脂肪(fang)酸結(jie)合(he)蛋白(bai)(bai)(bai)(bai)1(FABP1)捕獲(huo)抗(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包(bao)被微(wei)孔(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，依(yi)次(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)入標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)體(ti)(ti)(ti)，經過(guo)(guo)(guo)(guo)(guo)(guo)溫(wen)育并徹底(di)洗滌。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)底(di)物(wu)(wu)(wu)(wu)TMB顯(xian)(xian)色(se)(se)(se)(se)，TMB在(zai)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)酶(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)下(xia)(xia)轉化(hua)成藍(lan)色(se)(se)(se)(se)，并在(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)下(xia)(xia)轉化(hua)成＊終的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)色(se)(se)(se)(se)。顏色(se)(se)(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂肪(fang)酸結(jie)合(he)蛋白(bai)(bai)(bai)(bai)1(FABP1)呈正(zheng)相(xiang)(xiang)(xiang)關。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)在(zai)450nm 波長下(xia)(xia)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)吸(xi)光(guang)度(du)(du)(du)(du)(du)(du)（OD 值(zhi)），計(ji)(ji)(ji)算(suan)(suan)(suan)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)。保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)條件(jian)及有(you)效(xiao)期(qi)(qi)本(ben)(ben)(ben)試(shi)(shi)(shi)劑(ji)盒(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)體(ti)(ti)(ti)外定(ding)量(liang)(liang)(liang)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)血清、血漿(jiang)、組織勻(yun)漿(jiang)及相(xiang)(xiang)(xiang)關液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)樣(yang)(yang)(yang)本(ben)(ben)(ben)中(zhong)(zhong)脂肪(fang)酸結(jie)合(he)蛋白(bai)(bai)(bai)(bai)1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)(liang)。有(you)效(xiao)期(qi)(qi)：6個月保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)條件(jian)：2-8℃小(xiao)(xiao)(xiao)鼠可溶(rong)(rong)性(xing)髓(sui)系(xi)細胞(bao)觸(chu)發受(shou)體(ti)(ti)(ti)-2（sTREM-2）ELISA 檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)劑(ji)盒(he)(he)(he)一、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集(ji)及保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun) 1.血清：全血標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)請(qing)于(yu)(yu)(yu)(yu)(yu)室溫(wen)放置(zhi)2小(xiao)(xiao)(xiao)時(shi)(shi)(shi)或(huo)(huo)(huo)(huo)4℃過(guo)(guo)(guo)(guo)(guo)(guo)一晚后(hou)(hou)(hou)(hou)于(yu)(yu)(yu)(yu)(yu)1000 x g離心(xin)20分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)，取(qu)上(shang)清即(ji)可檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放于(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)，但應(ying)(ying)(ying)避(bi)免(mian)(mian)反(fan)(fan)復凍(dong)融(rong)。 2.血漿(jiang)：可用(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)肝素(su)(su)(su)(su)(su)作(zuo)(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)抗(kang)凝(ning)劑(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)采(cai)集(ji)后(hou)(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)內(nei)(nei)(nei)(nei)(nei)于(yu)(yu)(yu)(yu)(yu)2 - 8° C 1000 x g離心(xin)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)，或(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放于(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)，但應(ying)(ying)(ying)避(bi)免(mian)(mian)反(fan)(fan)復凍(dong)融(rong)。 3.細胞(bao)培養物(wu)(wu)(wu)(wu)上(shang)清或(huo)(huo)(huo)(huo)其它(ta)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)：1000 x g離心(xin)20分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)，取(qu)上(shang)清即(ji)可檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放于(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)，但應(ying)(ying)(ying)避(bi)免(mian)(mian)反(fan)(fan)復凍(dong)融(rong)。 注：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)溶(rong)(rong)血會影響檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)，因此(ci)溶(rong)(rong)血標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)不(bu)(bu)(bu)(bu)(bu)宜進行此(ci)項檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。二、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)   首先通(tong)過(guo)(guo)(guo)(guo)(guo)(guo)文(wen)獻檢(jian)(jian)(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)式了(le)(le)解(jie)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致(zhi)含量(liang)(liang)(liang)，確(que)定(ding)適(shi)當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)(bei)(bei)數(shu)(shu)。只(zhi)有(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)曲(qu)(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范(fan)圍內(nei)(nei)(nei)(nei)(nei)，檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果(guo)才(cai)是準(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)，應(ying)(ying)(ying)做(zuo)(zuo)好(hao)(hao)詳細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)錄。計(ji)(ji)(ji)算(suan)(suan)(suan)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)時(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)了(le)(le)“N”倍(bei)(bei)(bei)(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)應(ying)(ying)(ying)再(zai)乘(cheng)(cheng)(cheng)以(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)：2瓶，每(mei)(mei)(mei)瓶臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)1ml，蓋(gai)好(hao)(hao)后(hou)(hou)(hou)(hou)靜置(zhi)10分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)以(yi)上(shang)，然后(hou)(hou)(hou)(hou)反(fan)(fan)復顛倒/搓動以(yi)助溶(rong)(rong)解(jie)，其濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)300 pg/ml，做(zuo)(zuo)系(xi)列(lie)倍(bei)(bei)(bei)(bei)(bei)比(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)直接(jie)作(zuo)(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)0 pg/ml，臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)。 如(ru)(ru)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取(qu)0.5ml（不(bu)(bu)(bu)(bu)(bu)要(yao)(yao)少于(yu)(yu)(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)述(shu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)入含0.5ml樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)中(zhong)(zhong)，混(hun)(hun)(hun)勻(yun)即(ji)可，其余濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)以(yi)此(ci)類(lei)推(tui)(tui)。2.生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)： 臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)(gen)(gen)據預(yu)先計(ji)(ji)(ji)算(suan)(suan)(suan)好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)驗所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際(ji)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)加(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(li)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)，輕輕混(hun)(hun)(hun)勻(yun)，在(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一小(xiao)(xiao)(xiao)時(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)。3.辣(la)根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)(qin)和(he)(he)素(su)(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)： 臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)辣(la)根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)(qin)和(he)(he)素(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)(gen)(gen)據預(yu)先計(ji)(ji)(ji)算(suan)(suan)(suan)好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)驗所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際(ji)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl辣(la)根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)(qin)和(he)(he)素(su)(su)(su)(su)(su)加(jia)(jia)(jia)990μl辣(la)根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)(qin)和(he)(he)素(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(li)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)，輕輕混(hun)(hun)(hun)勻(yun)，在(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一小(xiao)(xiao)(xiao)時(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)。 操(cao)(cao)作(zuo)(zuo)(zuo)(zuo)步驟 實(shi)驗開(kai)始前(qian)(qian)(qian)，請(qing)提前(qian)(qian)(qian)配(pei)(pei)置(zhi)好(hao)(hao)所有(you)試(shi)(shi)(shi)劑(ji)，試(shi)(shi)(shi)劑(ji)或(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)，均需(xu)混(hun)(hun)(hun)勻(yun)，混(hun)(hun)(hun)勻(yun)時(shi)(shi)(shi)盡量(liang)(liang)(liang)避(bi)免(mian)(mian)起(qi)泡。每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)該(gai)(gai)做(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)曲(qu)(qu)(qu)線(xian)(xian)。如(ru)(ru)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)過(guo)(guo)(guo)(guo)(guo)(guo)高時(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)進行稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)使(shi)(shi)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合(he)試(shi)(shi)(shi)劑(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)圍。 小(xiao)(xiao)(xiao)鼠可溶(rong)(rong)性(xing)髓(sui)系(xi)細胞(bao)觸(chu)發受(shou)體(ti)(ti)(ti)-2（sTREM-2）ELISA 檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)劑(ji)盒(he)(he)(he)三、操(cao)(cao)作(zuo)(zuo)(zuo)(zuo)步驟1.加(jia)(jia)(jia)樣(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)設空白(bai)(bai)(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)孔(kong)(kong)(kong)(kong)(kong)、待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)。空白(bai)(bai)(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注意(yi)不(bu)(bu)(bu)(bu)(bu)要(yao)(yao)有(you)氣泡，加(jia)(jia)(jia)樣(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)于(yu)(yu)(yu)(yu)(yu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)底(di)部(bu)，盡量(liang)(liang)(liang)不(bu)(bu)(bu)(bu)(bu)觸(chu)及孔(kong)(kong)(kong)(kong)(kong)壁，輕輕晃動混(hun)(hun)(hun)勻(yun)，酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)(ban)加(jia)(jia)(jia)上(shang)蓋(gai)或(huo)(huo)(huo)(huo)覆(fu)膜，37℃反(fan)(fan)應(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)。 為(wei)(wei)(wei)(wei)保(bao)(bao)(bao)(bao)證實(shi)驗結(jie)果(guo)有(you)效(xiao)性(xing)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)驗請(qing)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)。2.棄(qi)去液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干，不(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗滌。每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)工作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye) 100μl（取(qu)1μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)加(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(li)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)，輕輕混(hun)(hun)(hun)勻(yun)，在(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一小(xiao)(xiao)(xiao)時(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)。 3.溫(wen)育60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)后(hou)(hou)(hou)(hou)，棄(qi)去孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干，洗板(ban)(ban)(ban)(ban)(ban)(ban)3次(ci)(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)浸泡1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)干。 4.每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)辣(la)根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)(qin)和(he)(he)素(su)(su)(su)(su)(su)工作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)（同生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)工作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)。 5.溫(wen)育60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)后(hou)(hou)(hou)(hou)，棄(qi)去孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干，洗板(ban)(ban)(ban)(ban)(ban)(ban)5次(ci)(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)浸泡1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)干。 6.依(yi)序(xu)每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)底(di)物(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)光(guang)顯(xian)(xian)色(se)(se)(se)(se)（30分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)內(nei)(nei)(nei)(nei)(nei)，此(ci)時(shi)(shi)(shi)肉眼(yan)可見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)有(you)明(ming)顯(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)(du)(du)(du)藍(lan)色(se)(se)(se)(se)，后(hou)(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)梯度(du)(du)(du)(du)(du)(du)不(bu)(bu)(bu)(bu)(bu)明(ming)顯(xian)(xian)，即(ji)可終止）。 7.依(yi)序(xu)每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)終止溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)50μl，終止反(fan)(fan)應(ying)(ying)(ying)（此(ci)時(shi)(shi)(shi)藍(lan)色(se)(se)(se)(se)立轉黃(huang)色(se)(se)(se)(se)）。終止液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)入順(shun)序(xu)應(ying)(ying)(ying)盡量(liang)(liang)(liang)與(yu)底(di)物(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)入順(shun)序(xu)相(xiang)(xiang)(xiang)同。為(wei)(wei)(wei)(wei)了(le)(le)保(bao)(bao)(bao)(bao)證實(shi)驗結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)確(que)性(xing)，底(di)物(wu)(wu)(wu)(wu)反(fan)(fan)應(ying)(ying)(ying)時(shi)(shi)(shi)間到(dao)后(hou)(hou)(hou)(hou)應(ying)(ying)(ying)盡快(kuai)加(jia)(jia)(jia)入終止液(ye)(ye)(ye)(ye)(ye)。 8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)聯儀(yi)在(zai)450nm波長依(yi)序(xu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)各(ge)孔(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)密度(du)(du)(du)(du)(du)(du)（OD值(zhi)）。 在(zai)加(jia)(jia)(jia)終止液(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)以(yi)內(nei)(nei)(nei)(nei)(nei)進行檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。 四、試(shi)(shi)(shi)劑(ji)盒(he)(he)(he)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)小(xiao)(xiao)(xiao)技(ji)(ji)巧1. 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)初次(ci)(ci)(ci)(ci)(ci)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)劑(ji)盒(he)(he)(he)時(shi)(shi)(shi)，應(ying)(ying)(ying)將(jiang)各(ge)種試(shi)(shi)(shi)劑(ji)管(guan)離心(xin)數(shu)(shu)分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)，以(yi)便試(shi)(shi)(shi)劑(ji)集(ji)中(zhong)(zhong)到(dao)管(guan)底(di)。2. 每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)驗留一孔(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)空白(bai)(bai)(bai)(bai)調(diao)零(ling)孔(kong)(kong)(kong)(kong)(kong)，該(gai)(gai)孔(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)(bu)(bu)加(jia)(jia)(jia)任(ren)何試(shi)(shi)(shi)劑(ji)，只(zhi)是**加(jia)(jia)(jia)底(di)物(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)及2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)時(shi)(shi)(shi)先用(yong)(yong)(yong)(yong)(yong)(yong)(yong)此(ci)孔(kong)(kong)(kong)(kong)(kong)調(diao)OD值(zhi)至(zhi)零(ling)。 3. 為(wei)(wei)(wei)(wei)防止樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸(zheng)發，試(shi)(shi)(shi)驗時(shi)(shi)(shi)將(jiang)反(fan)(fan)應(ying)(ying)(ying)板(ban)(ban)(ban)(ban)(ban)(ban)放于(yu)(yu)(yu)(yu)(yu)鋪有(you)濕布(bu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)(he)(he)內(nei)(nei)(nei)(nei)(nei)，酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)(ban)加(jia)(jia)(jia)上(shang)蓋(gai)或(huo)(huo)(huo)(huo)覆(fu)膜。 4. 未使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)(ban)或(huo)(huo)(huo)(huo)者(zhe)試(shi)(shi)(shi)劑(ji)，請(qing)于(yu)(yu)(yu)(yu)(yu)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)工作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)、辣(la)根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)(qin)和(he)(he)素(su)(su)(su)(su)(su)工作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)請(qing)依(yi)據所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)配(pei)(pei)置(zhi)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)勿重復使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)已(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)工作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)、辣(la)根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)(qin)和(he)(he)素(su)(su)(su)(su)(su)工作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)。 5. 建議檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)均設雙孔(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)，以(yi)保(bao)(bao)(bao)(bao)證檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)確(que)性(xing)。 洗板(ban)(ban)(ban)(ban)(ban)(ban)方(fang)法(fa)(fa) 手工洗板(ban)(ban)(ban)(ban)(ban)(ban)方(fang)法(fa)(fa)：吸(xi)去（不(bu)(bu)(bu)(bu)(bu)可觸(chu)及板(ban)(ban)(ban)(ban)(ban)(ban)壁）或(huo)(huo)(huo)(huo)甩(shuai)掉酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)(ban)內(nei)(nei)(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)；在(zai)實(shi)驗臺上(shang)鋪墊(dian)幾(ji)層吸(xi)水紙，酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)(ban)朝(chao)下(xia)(xia)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)力拍幾(ji)次(ci)(ci)(ci)(ci)(ci)；將(jiang)推(tui)(tui)薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗滌緩沖(chong)液(ye)(ye)(ye)(ye)(ye)至(zhi)少0.3ml注入孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)，浸泡1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)。根(gen)(gen)(gen)據需(xu)要(yao)(yao)，重復此(ci)過(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)數(shu)(shu)次(ci)(ci)(ci)(ci)(ci)。 自動洗板(ban)(ban)(ban)(ban)(ban)(ban)：如(ru)(ru)果(guo)有(you)自動洗板(ban)(ban)(ban)(ban)(ban)(ban)機，應(ying)(ying)(ying)在(zai)熟練使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)(hou)再(zai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)到(dao)正(zheng)式實(shi)驗過(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)。 計(ji)(ji)(ji)算(suan)(suan)(suan) 以(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)橫(heng)(heng)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對(dui)(dui)數(shu)(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)為(wei)(wei)(wei)(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通(tong)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)半對(dui)(dui)數(shu)(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)繪(hui)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)曲(qu)(qu)(qu)線(xian)(xian)，根(gen)(gen)(gen)據樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)曲(qu)(qu)(qu)線(xian)(xian)查出(chu)相(xiang)(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)；再(zai)乘(cheng)(cheng)(cheng)以(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)(bei)(bei)數(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)計(ji)(ji)(ji)算(suan)(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)曲(qu)(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)回歸方(fang)程(cheng)(cheng)式，將(jiang)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代入方(fang)程(cheng)(cheng)式，計(ji)(ji)(ji)算(suan)(suan)(suan)出(chu)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)，再(zai)乘(cheng)(cheng)(cheng)以(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)(bei)(bei)數(shu)(shu)，即(ji)為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際(ji)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)。 五、技(ji)(ji)術(shu)小(xiao)(xiao)(xiao)提示1.當混(hun)(hun)(hun)合(he)蛋白(bai)(bai)(bai)(bai)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)應(ying)(ying)(ying)盡量(liang)(liang)(liang)輕緩，避(bi)免(mian)(mian)起(qi)泡。2. 洗滌過(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)非常重要(yao)(yao)，不(bu)(bu)(bu)(bu)(bu)充分(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗滌易造成假陽(yang)性(xing)。 3. 一次(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)樣(yang)(yang)(yang)時(shi)(shi)(shi)間控制(zhi)(zhi)(zhi)(zhi)在(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)內(nei)(nei)(nei)(nei)(nei)，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)數(shu)(shu)量(liang)(liang)(liang)多，推(tui)(tui)薦使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)樣(yang)(yang)(yang)。 4. 請(qing)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)做(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)曲(qu)(qu)(qu)線(xian)(xian)，做(zuo)(zuo)復孔(kong)(kong)(kong)(kong)(kong)。 5. 如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)中(zhong)(zhong)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)質含量(liang)(liang)(liang)過(guo)(guo)(guo)(guo)(guo)(guo)高，請(qing)先稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)再(zai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)，計(ji)(ji)(ji)算(suan)(suan)(suan)時(shi)(shi)(shi)請(qing)乘(cheng)(cheng)(cheng)以(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)(bei)(bei)數(shu)(shu)。 6. 在(zai)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)工作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)，請(qing)以(yi)相(xiang)(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)，不(bu)(bu)(bu)(bu)(bu)能混(hun)(hun)(hun)淆。 7. 底(di)物(wu)(wu)(wu)(wu)請(qing)避(bi)光(guang)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。 8. 不(bu)(bu)(bu)(bu)(bu)要(yao)(yao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)其它(ta)生(sheng)(sheng)(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)替換(huan)試(shi)(shi)(shi)劑(ji)盒(he)(he)(he)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)。 六(liu)、注意(yi)事項1.收集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)前(qian)(qian)(qian)必須清楚要(yao)(yao)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成份是否足夠穩定(ding)。對(dui)(dui)收集(ji)后(hou)(hou)(hou)(hou)當天進行檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)，儲存(cun)(cun)(cun)(cun)在(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)有(you)特殊原(yuan)因需(xu)要(yao)(yao)周期(qi)(qi)收集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)， 將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)及時(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)后(hou)(hou)(hou)(hou)放在(zai)-20 ℃或(huo)(huo)(huo)(huo)-70 ℃條件(jian)下(xia)(xia)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。避(bi)免(mian)(mian)反(fan)(fan)復凍(dong)融(rong)。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)2 -8 ℃可保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)48 小(xiao)(xiao)(xiao)時(shi)(shi)(shi)，-20 ℃可保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)1 個月。-70度(du)(du)(du)(du)(du)(du)可保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)6 個月。3.部(bu)分(fen)(fen)(fen)(fen)(fen)(fen)激素(su)(su)(su)(su)(su)類(lei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)需(xu)添加(jia)(jia)(jia)抑(yi)肽酶(mei)。 4.計(ji)(ji)(ji)算(suan)(suan)(suan)方(fang)法(fa)(fa)：以(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)橫(heng)(heng)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)為(wei)(wei)(wei)(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)繪(hui)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)曲(qu)(qu)(qu)線(xian)(xian)，根(gen)(gen)(gen)據樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)曲(qu)(qu)(qu)線(xian)(xian)查出(chu)相(xiang)(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)；再(zai)乘(cheng)(cheng)(cheng)以(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)(bei)(bei)數(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)計(ji)(ji)(ji)算(suan)(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)曲(qu)(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)回歸方(fang)程(cheng)(cheng)式，將(jiang)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代入方(fang)程(cheng)(cheng)式，計(ji)(ji)(ji)算(suan)(suan)(suan)出(chu)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)，再(zai)乘(cheng)(cheng)(cheng)以(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)(bei)(bei)數(shu)(shu)，即(ji)為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際(ji)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)。 七、試(shi)(shi)(shi)劑(ji)盒(he)(he)(he)性(xing)能1.樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)性(xing)回歸與(yu)預(yu)期(qi)(qi)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)相(xiang)(xiang)(xiang)關系(xi)數(shu)(shu)R值(zhi)為(wei)(wei)(wei)(wei)0.92以(yi)上(shang)。2.批內(nei)(nei)(nei)(nei)(nei)與(yu)批見應(ying)(ying)(ying)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)小(xiao)(xiao)(xiao)于(yu)(yu)(yu)(yu)(yu)9%和(he)(he)15% 八、保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)條件(jian)及有(you)效(xiao)期(qi)(qi)1.試(shi)(shi)(shi)劑(ji)盒(he)(he)(he)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)：2-8℃。 2．有(you)效(xiao)期(qi)(qi)：6個月

 小(xiao)(xiao)(xiao)鼠磷(lin)酸(suan)(suan)化(hua)(hua)(hua)(hua)(hua) Tau 蛋(dan)白（p-Tau）ELISA 檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)實驗(yan)原(yuan)(yuan)(yuan)理   試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)一步夾心(xin)法(fa)酶(mei)聯(lian)免(mian)(mian)(mian)疫吸附試(shi)(shi)(shi)驗(yan)（ELISA）。往預(yu)(yu)先(xian)包被脂肪(fang)(fang)酸(suan)(suan)結(jie)(jie)合蛋(dan)白1(FABP1)捕獲抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，依(yi)次(ci)(ci)(ci)加(jia)(jia)(jia)入標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)，經過(guo)(guo)(guo)溫育(yu)(yu)(yu)并(bing)徹底洗(xi)滌(di)(di)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯色(se)(se)，TMB在(zai)(zai)過(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)(hua)(hua)(hua)下轉(zhuan)化(hua)(hua)(hua)(hua)(hua)成(cheng)藍色(se)(se)，并(bing)在(zai)(zai)酸(suan)(suan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)下轉(zhuan)化(hua)(hua)(hua)(hua)(hua)成(cheng)＊終(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)(se)。顏色(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺(qian)和(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂肪(fang)(fang)酸(suan)(suan)結(jie)(jie)合蛋(dan)白1(FABP1)呈正(zheng)相(xiang)(xiang)(xiang)關(guan)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)在(zai)(zai)450nm 波長下測(ce)(ce)(ce)定(ding)(ding)吸光(guang)度(du)(du)(du)(du)(du)（OD 值(zhi)(zhi)），計(ji)算樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)。保(bao)存(cun)(cun)(cun)(cun)(cun)條(tiao)件(jian)及(ji)有(you)(you)效(xiao)(xiao)期本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)外定(ding)(ding)量(liang)(liang)(liang)(liang)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)血(xue)清、血(xue)漿、組織勻(yun)漿及(ji)相(xiang)(xiang)(xiang)關(guan)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)脂肪(fang)(fang)酸(suan)(suan)結(jie)(jie)合蛋(dan)白1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)(liang)(liang)。有(you)(you)效(xiao)(xiao)期：6個月(yue)保(bao)存(cun)(cun)(cun)(cun)(cun)條(tiao)件(jian)：2-8℃小(xiao)(xiao)(xiao)鼠磷(lin)酸(suan)(suan)化(hua)(hua)(hua)(hua)(hua) Tau 蛋(dan)白（p-Tau）ELISA 檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)一、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集(ji)(ji)(ji)及(ji)保(bao)存(cun)(cun)(cun)(cun)(cun) 1.血(xue)清：全血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)請(qing)(qing)(qing)于(yu)(yu)室溫放(fang)置(zhi)2小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)4℃過(guo)(guo)(guo)一晚(wan)后(hou)(hou)(hou)于(yu)(yu)1000 x g離(li)心(xin)20分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，取(qu)(qu)上(shang)清即(ji)可(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)，或(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)-20℃或(huo)(huo)-80℃保(bao)存(cun)(cun)(cun)(cun)(cun)，但(dan)應(ying)避(bi)(bi)(bi)免(mian)(mian)(mian)反(fan)(fan)復(fu)(fu)凍(dong)(dong)融。 2.血(xue)漿：可(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)肝素(su)(su)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)為(wei)抗(kang)(kang)(kang)凝劑(ji)(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)采(cai)集(ji)(ji)(ji)后(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)于(yu)(yu)2 - 8° C 1000 x g離(li)心(xin)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，或(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)-20℃或(huo)(huo)-80℃保(bao)存(cun)(cun)(cun)(cun)(cun)，但(dan)應(ying)避(bi)(bi)(bi)免(mian)(mian)(mian)反(fan)(fan)復(fu)(fu)凍(dong)(dong)融。 3.細胞培養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)清或(huo)(huo)其它(ta)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)：1000 x g離(li)心(xin)20分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，取(qu)(qu)上(shang)清即(ji)可(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)，或(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)-20℃或(huo)(huo)-80℃保(bao)存(cun)(cun)(cun)(cun)(cun)，但(dan)應(ying)避(bi)(bi)(bi)免(mian)(mian)(mian)反(fan)(fan)復(fu)(fu)凍(dong)(dong)融。 注(zhu)(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)溶(rong)血(xue)會影響檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)結(jie)(jie)果(guo)，因(yin)(yin)此(ci)(ci)溶(rong)血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)不(bu)宜(yi)進(jin)行此(ci)(ci)項(xiang)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)。二、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)(yuan)則(ze)(ze)   首先(xian)通(tong)過(guo)(guo)(guo)文獻(xian)檢(jian)(jian)(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)(fang)式(shi)了解(jie)(jie)待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含量(liang)(liang)(liang)(liang)，確(que)(que)定(ding)(ding)適當(dang)(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)。只有(you)(you)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍內(nei)，檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)(jie)果(guo)才是準(zhun)(zhun)(zhun)(zhun)確(que)(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)，應(ying)做(zuo)好(hao)詳細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)(ji)(ji)錄。計(ji)算濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)應(ying)再(zai)乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)(yuan)則(ze)(ze)：2瓶(ping)(ping)，每(mei)(mei)(mei)(mei)瓶(ping)(ping)臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)(zhi)1ml，蓋好(hao)后(hou)(hou)(hou)靜置(zhi)10分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)(yi)上(shang)，然后(hou)(hou)(hou)反(fan)(fan)復(fu)(fu)顛倒/搓(cuo)動(dong)以(yi)(yi)(yi)(yi)(yi)(yi)助(zhu)溶(rong)解(jie)(jie)，其濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)為(wei)300 pg/ml，做(zuo)系列倍(bei)比(bi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)直接作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)為(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)0 pg/ml，臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 如(ru)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取(qu)(qu)0.5ml（不(bu)要(yao)(yao)少于(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)入含0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中(zhong)(zhong)(zhong)(zhong)(zhong)，混(hun)(hun)(hun)(hun)勻(yun)即(ji)可(ke)(ke)，其余濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)以(yi)(yi)(yi)(yi)(yi)(yi)此(ci)(ci)類推(tui)(tui)(tui)。2.生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)(yuan)則(ze)(ze)： 臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)根(gen)據(ju)預(yu)(yu)先(xian)計(ji)算好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)實驗(yan)所(suo)(suo)需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)100μl），實際(ji)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)多(duo)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)10μl生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)加(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混(hun)(hun)(hun)(hun)勻(yun)，在(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。3.辣根(gen)過(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)(yuan)則(ze)(ze)： 臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)辣根(gen)過(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)根(gen)據(ju)預(yu)(yu)先(xian)計(ji)算好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)實驗(yan)所(suo)(suo)需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)100μl），實際(ji)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)多(duo)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)10μl辣根(gen)過(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)加(jia)(jia)(jia)990μl辣根(gen)過(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混(hun)(hun)(hun)(hun)勻(yun)，在(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 操作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)步驟 實驗(yan)開始前(qian)(qian)(qian)(qian)，請(qing)(qing)(qing)提(ti)前(qian)(qian)(qian)(qian)配(pei)(pei)(pei)(pei)置(zhi)好(hao)所(suo)(suo)有(you)(you)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)或(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)，均需混(hun)(hun)(hun)(hun)勻(yun)，混(hun)(hun)(hun)(hun)勻(yun)時(shi)(shi)(shi)(shi)(shi)(shi)盡(jin)量(liang)(liang)(liang)(liang)避(bi)(bi)(bi)免(mian)(mian)(mian)起泡(pao)。每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)都(dou)應(ying)該做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)。如(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)過(guo)(guo)(guo)高時(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)進(jin)行稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)(yi)(yi)使(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)范圍。 小(xiao)(xiao)(xiao)鼠磷(lin)酸(suan)(suan)化(hua)(hua)(hua)(hua)(hua) Tau 蛋(dan)白（p-Tau）ELISA 檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)三、操作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)步驟1.加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)設空(kong)(kong)白孔(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)孔(kong)、待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)。空(kong)(kong)白孔(kong)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)(zhu)意(yi)不(bu)要(yao)(yao)有(you)(you)氣(qi)泡(pao)，加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)于(yu)(yu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)底部，盡(jin)量(liang)(liang)(liang)(liang)不(bu)觸及(ji)孔(kong)壁(bi)(bi)，輕(qing)(qing)輕(qing)(qing)晃(huang)動(dong)混(hun)(hun)(hun)(hun)勻(yun)，酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)加(jia)(jia)(jia)上(shang)蓋或(huo)(huo)覆(fu)膜，37℃反(fan)(fan)應(ying)120分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 為(wei)保(bao)證實驗(yan)結(jie)(jie)果(guo)有(you)(you)效(xiao)(xiao)性(xing)(xing)(xing)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)實驗(yan)請(qing)(qing)(qing)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)液(ye)(ye)(ye)(ye)(ye)。2.棄去(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)，甩(shuai)干(gan)，不(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)滌(di)(di)。每(mei)(mei)(mei)(mei)孔(kong)加(jia)(jia)(jia)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye) 100μl（取(qu)(qu)1μl生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)加(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混(hun)(hun)(hun)(hun)勻(yun)，在(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 3.溫育(yu)(yu)(yu)60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)，棄去(qu)孔(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)，甩(shuai)干(gan)，洗(xi)板(ban)(ban)3次(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)浸(jin)泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)，甩(shuai)干(gan)。 4.每(mei)(mei)(mei)(mei)孔(kong)加(jia)(jia)(jia)辣根(gen)過(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)（同生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 5.溫育(yu)(yu)(yu)60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)，棄去(qu)孔(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)，甩(shuai)干(gan)，洗(xi)板(ban)(ban)5次(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)浸(jin)泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)，甩(shuai)干(gan)。 6.依(yi)序每(mei)(mei)(mei)(mei)孔(kong)加(jia)(jia)(jia)底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)(bi)(bi)光(guang)顯色(se)(se)（30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)，此(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)肉(rou)眼可(ke)(ke)見(jian)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)(qian)3-4孔(kong)有(you)(you)明(ming)顯的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)(du)(du)藍色(se)(se)，后(hou)(hou)(hou)3-4孔(kong)梯度(du)(du)(du)(du)(du)不(bu)明(ming)顯，即(ji)可(ke)(ke)終(zhong)(zhong)(zhong)止(zhi)(zhi)(zhi)）。 7.依(yi)序每(mei)(mei)(mei)(mei)孔(kong)加(jia)(jia)(jia)終(zhong)(zhong)(zhong)止(zhi)(zhi)(zhi)溶(rong)液(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)(zhong)止(zhi)(zhi)(zhi)反(fan)(fan)應(ying)（此(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)藍色(se)(se)立轉(zhuan)黃色(se)(se)）。終(zhong)(zhong)(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)入順序應(ying)盡(jin)量(liang)(liang)(liang)(liang)與底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)入順序相(xiang)(xiang)(xiang)同。為(wei)了保(bao)證實驗(yan)結(jie)(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)確(que)(que)性(xing)(xing)(xing)，底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)(fan)應(ying)時(shi)(shi)(shi)(shi)(shi)(shi)間(jian)到后(hou)(hou)(hou)應(ying)盡(jin)快加(jia)(jia)(jia)入終(zhong)(zhong)(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)。 8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)聯(lian)儀(yi)在(zai)(zai)450nm波長依(yi)序測(ce)(ce)(ce)量(liang)(liang)(liang)(liang)各孔(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)密度(du)(du)(du)(du)(du)（OD值(zhi)(zhi)）。 在(zai)(zai)加(jia)(jia)(jia)終(zhong)(zhong)(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)(yi)內(nei)進(jin)行檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)。 四、試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)小(xiao)(xiao)(xiao)技巧1. 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)初次(ci)(ci)(ci)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)時(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)將(jiang)(jiang)各種試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)管離(li)心(xin)數(shu)(shu)(shu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)集(ji)(ji)(ji)中(zhong)(zhong)(zhong)(zhong)(zhong)到管底。2. 每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)實驗(yan)留(liu)一孔(kong)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)為(wei)空(kong)(kong)白調(diao)(diao)零孔(kong)，該孔(kong)不(bu)加(jia)(jia)(jia)任(ren)何試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，只是**加(jia)(jia)(jia)底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)(ye)及(ji)2N H2SO4。測(ce)(ce)(ce)量(liang)(liang)(liang)(liang)時(shi)(shi)(shi)(shi)(shi)(shi)先(xian)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)此(ci)(ci)孔(kong)調(diao)(diao)OD值(zhi)(zhi)至(zhi)(zhi)零。 3. 為(wei)防止(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)驗(yan)時(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)反(fan)(fan)應(ying)板(ban)(ban)放(fang)于(yu)(yu)鋪(pu)有(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)內(nei)，酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)加(jia)(jia)(jia)上(shang)蓋或(huo)(huo)覆(fu)膜。 4. 未(wei)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)或(huo)(huo)者(zhe)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，請(qing)(qing)(qing)于(yu)(yu)2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)、辣根(gen)過(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)請(qing)(qing)(qing)依(yi)據(ju)所(suo)(suo)需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)置(zhi)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)(qing)勿重復(fu)(fu)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)已(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)或(huo)(huo)、辣根(gen)過(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)。 5. 建議檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)均設雙孔(kong)測(ce)(ce)(ce)定(ding)(ding)，以(yi)(yi)(yi)(yi)(yi)(yi)保(bao)證檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)結(jie)(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)確(que)(que)性(xing)(xing)(xing)。 洗(xi)板(ban)(ban)方(fang)(fang)(fang)法(fa) 手(shou)工(gong)(gong)(gong)洗(xi)板(ban)(ban)方(fang)(fang)(fang)法(fa)：吸去(qu)（不(bu)可(ke)(ke)觸及(ji)板(ban)(ban)壁(bi)(bi)）或(huo)(huo)甩(shuai)掉酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)內(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)；在(zai)(zai)實驗(yan)臺上(shang)鋪(pu)墊幾層(ceng)吸水紙(zhi)(zhi)，酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)朝下用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)力(li)拍幾次(ci)(ci)(ci)；將(jiang)(jiang)推(tui)(tui)(tui)薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌(di)(di)緩沖液(ye)(ye)(ye)(ye)(ye)至(zhi)(zhi)少0.3ml注(zhu)(zhu)入孔(kong)內(nei)，浸(jin)泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。根(gen)據(ju)需要(yao)(yao)，重復(fu)(fu)此(ci)(ci)過(guo)(guo)(guo)程數(shu)(shu)(shu)次(ci)(ci)(ci)。 自動(dong)洗(xi)板(ban)(ban)：如(ru)果(guo)有(you)(you)自動(dong)洗(xi)板(ban)(ban)機，應(ying)在(zai)(zai)熟練(lian)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)再(zai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)到正(zheng)式(shi)實驗(yan)過(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)。 計(ji)算 以(yi)(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)為(wei)橫坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對(dui)數(shu)(shu)(shu)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)為(wei)縱坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普(pu)通(tong)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)半對(dui)數(shu)(shu)(shu)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)(zhi)上(shang)繪出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)，根(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)查出(chu)(chu)(chu)相(xiang)(xiang)(xiang)應(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)；再(zai)乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)；或(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)與OD值(zhi)(zhi)計(ji)算出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)回歸方(fang)(fang)(fang)程式(shi)，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)代入方(fang)(fang)(fang)程式(shi)，計(ji)算出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)，再(zai)乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)，即(ji)為(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實際(ji)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)。 五(wu)、技術小(xiao)(xiao)(xiao)提(ti)示1.當(dang)(dang)混(hun)(hun)(hun)(hun)合蛋(dan)白溶(rong)液(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)盡(jin)量(liang)(liang)(liang)(liang)輕(qing)(qing)緩，避(bi)(bi)(bi)免(mian)(mian)(mian)起泡(pao)。2. 洗(xi)滌(di)(di)過(guo)(guo)(guo)程非常重要(yao)(yao)，不(bu)充分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌(di)(di)易(yi)造(zao)成(cheng)假(jia)陽(yang)性(xing)(xing)(xing)。 3. 一次(ci)(ci)(ci)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)間(jian)控(kong)制(zhi)(zhi)(zhi)在(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)多(duo)，推(tui)(tui)(tui)薦使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)。 4. 請(qing)(qing)(qing)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)測(ce)(ce)(ce)定(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)，做(zuo)復(fu)(fu)孔(kong)。 5. 如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)待測(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)(liang)(liang)(liang)過(guo)(guo)(guo)高，請(qing)(qing)(qing)先(xian)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)再(zai)測(ce)(ce)(ce)定(ding)(ding)，計(ji)算時(shi)(shi)(shi)(shi)(shi)(shi)請(qing)(qing)(qing)乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)。 6. 在(zai)(zai)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)溶(rong)液(ye)(ye)(ye)(ye)(ye)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)(qing)(qing)以(yi)(yi)(yi)(yi)(yi)(yi)相(xiang)(xiang)(xiang)應(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，不(bu)能(neng)混(hun)(hun)(hun)(hun)淆。 7. 底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)(qing)避(bi)(bi)(bi)光(guang)保(bao)存(cun)(cun)(cun)(cun)(cun)。 8. 不(bu)要(yao)(yao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)其它(ta)生(sheng)(sheng)(sheng)(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)替換試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)。 六(liu)、注(zhu)(zhu)意(yi)事項(xiang)1.收集(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)前(qian)(qian)(qian)(qian)必須清楚要(yao)(yao)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)份(fen)是否(fou)足夠(gou)穩定(ding)(ding)。對(dui)收集(ji)(ji)(ji)后(hou)(hou)(hou)當(dang)(dang)天進(jin)行檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)，儲(chu)存(cun)(cun)(cun)(cun)(cun)在(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)有(you)(you)特殊原(yuan)(yuan)(yuan)因(yin)(yin)需要(yao)(yao)周期收集(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)， 將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)及(ji)時(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)后(hou)(hou)(hou)放(fang)在(zai)(zai)-20 ℃或(huo)(huo)-70 ℃條(tiao)件(jian)下保(bao)存(cun)(cun)(cun)(cun)(cun)。避(bi)(bi)(bi)免(mian)(mian)(mian)反(fan)(fan)復(fu)(fu)凍(dong)(dong)融。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)保(bao)存(cun)(cun)(cun)(cun)(cun)48 小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)保(bao)存(cun)(cun)(cun)(cun)(cun)1 個月(yue)。-70度(du)(du)(du)(du)(du)可(ke)(ke)保(bao)存(cun)(cun)(cun)(cun)(cun)6 個月(yue)。3.部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)激素(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)需添加(jia)(jia)(jia)抑肽酶(mei)。 4.計(ji)算方(fang)(fang)(fang)法(fa)：以(yi)(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)為(wei)橫坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)為(wei)縱坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)(zhi)上(shang)繪出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)，根(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)查出(chu)(chu)(chu)相(xiang)(xiang)(xiang)應(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)；再(zai)乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)；或(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)與OD值(zhi)(zhi)計(ji)算出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)回歸方(fang)(fang)(fang)程式(shi)，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)代入方(fang)(fang)(fang)程式(shi)，計(ji)算出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)，再(zai)乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)，即(ji)為(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實際(ji)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)。 七(qi)、試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)性(xing)(xing)(xing)能(neng)1.樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)性(xing)(xing)(xing)回歸與預(yu)(yu)期濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)相(xiang)(xiang)(xiang)關(guan)系數(shu)(shu)(shu)R值(zhi)(zhi)為(wei)0.92以(yi)(yi)(yi)(yi)(yi)(yi)上(shang)。2.批內(nei)與批見(jian)應(ying)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)小(xiao)(xiao)(xiao)于(yu)(yu)9%和(he)(he)(he)15% 八(ba)、保(bao)存(cun)(cun)(cun)(cun)(cun)條(tiao)件(jian)及(ji)有(you)(you)效(xiao)(xiao)期1.試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)保(bao)存(cun)(cun)(cun)(cun)(cun)：2-8℃。 2．有(you)(you)效(xiao)(xiao)期：6個月(yue)

 小(xiao)鼠(shu)(shu)神經絲(si)蛋(dan)白(bai)(bai)(bai) L（NFL）ELISA 檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)實(shi)(shi)驗(yan)原(yuan)理   試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)采(cai)用(yong)(yong)雙(shuang)抗(kang)(kang)(kang)體(ti)(ti)(ti)一(yi)(yi)步(bu)夾(jia)心法(fa)(fa)(fa)酶(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)疫吸(xi)附(fu)試(shi)(shi)(shi)(shi)驗(yan)（ELISA）。往(wang)預先(xian)(xian)包被脂肪酸(suan)結(jie)合(he)蛋(dan)白(bai)(bai)(bai)1(FABP1)捕(bu)獲抗(kang)(kang)(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)(kong)中(zhong)，依(yi)次(ci)(ci)(ci)(ci)加(jia)(jia)(jia)入標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)體(ti)(ti)(ti)，經過(guo)(guo)溫育(yu)并(bing)徹底(di)(di)洗(xi)滌(di)。用(yong)(yong)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯(xian)色(se)，TMB在(zai)(zai)(zai)(zai)過(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)下(xia)(xia)轉(zhuan)(zhuan)化(hua)(hua)成藍色(se)，并(bing)在(zai)(zai)(zai)(zai)酸(suan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)下(xia)(xia)轉(zhuan)(zhuan)化(hua)(hua)成＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)。顏色(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂肪酸(suan)結(jie)合(he)蛋(dan)白(bai)(bai)(bai)1(FABP1)呈正相(xiang)(xiang)關。用(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)(zai)(zai)450nm 波(bo)長下(xia)(xia)測(ce)(ce)(ce)(ce)(ce)定(ding)吸(xi)光(guang)(guang)度(du)(du)(du)（OD 值(zhi)(zhi)(zhi)(zhi)），計算(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)。保(bao)存(cun)(cun)(cun)條件(jian)及(ji)有(you)(you)(you)(you)(you)效(xiao)(xiao)期本(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)用(yong)(yong)于(yu)(yu)(yu)體(ti)(ti)(ti)外定(ding)量(liang)(liang)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)血(xue)清、血(xue)漿、組(zu)織勻(yun)(yun)(yun)(yun)漿及(ji)相(xiang)(xiang)關液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)中(zhong)脂肪酸(suan)結(jie)合(he)蛋(dan)白(bai)(bai)(bai)1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)。有(you)(you)(you)(you)(you)效(xiao)(xiao)期：6個(ge)(ge)月(yue)保(bao)存(cun)(cun)(cun)條件(jian)：2-8℃小(xiao)鼠(shu)(shu)神經絲(si)蛋(dan)白(bai)(bai)(bai) L（NFL）ELISA 檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)一(yi)(yi)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集及(ji)保(bao)存(cun)(cun)(cun) 1.血(xue)清：全血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)請(qing)(qing)于(yu)(yu)(yu)室(shi)溫放置2小(xiao)時(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)(huo)4℃過(guo)(guo)一(yi)(yi)晚后(hou)(hou)(hou)于(yu)(yu)(yu)1000 x g離(li)心20分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，取上(shang)(shang)(shang)(shang)清即(ji)可(ke)(ke)(ke)(ke)(ke)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)存(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)(mian)(mian)反(fan)(fan)(fan)(fan)復凍融。 2.血(xue)漿：可(ke)(ke)(ke)(ke)(ke)用(yong)(yong)EDTA或(huo)(huo)(huo)(huo)肝素(su)(su)(su)(su)(su)作(zuo)(zuo)為(wei)(wei)(wei)抗(kang)(kang)(kang)凝劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)采(cai)集后(hou)(hou)(hou)30分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內于(yu)(yu)(yu)2 - 8° C 1000 x g離(li)心15分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)存(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)(mian)(mian)反(fan)(fan)(fan)(fan)復凍融。 3.細胞培養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)(shang)清或(huo)(huo)(huo)(huo)其(qi)(qi)(qi)它生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)：1000 x g離(li)心20分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，取上(shang)(shang)(shang)(shang)清即(ji)可(ke)(ke)(ke)(ke)(ke)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)存(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)(mian)(mian)反(fan)(fan)(fan)(fan)復凍融。 注：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)溶(rong)(rong)(rong)血(xue)會影響(xiang)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)(guo)，因(yin)此(ci)(ci)溶(rong)(rong)(rong)血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)不(bu)(bu)(bu)宜進行(xing)此(ci)(ci)項檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)。二、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)   首(shou)先(xian)(xian)通過(guo)(guo)文(wen)獻檢(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)式(shi)了解待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含量(liang)(liang)，確(que)(que)(que)定(ding)適當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)(shu)。只有(you)(you)(you)(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范(fan)(fan)圍內，檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果(guo)(guo)才是準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)(que)(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)程中(zhong)，應(ying)(ying)(ying)(ying)做好詳細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)(ji)(ji)錄。計算(suan)(suan)濃(nong)(nong)(nong)度(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)應(ying)(ying)(ying)(ying)再(zai)(zai)乘以(yi)(yi)(yi)(yi)(yi)(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)：2瓶，每(mei)(mei)(mei)瓶臨(lin)(lin)用(yong)(yong)前(qian)(qian)(qian)(qian)(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)至1ml，蓋(gai)好后(hou)(hou)(hou)靜置10分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)(yi)上(shang)(shang)(shang)(shang)，然(ran)后(hou)(hou)(hou)反(fan)(fan)(fan)(fan)復顛倒/搓動以(yi)(yi)(yi)(yi)(yi)(yi)助溶(rong)(rong)(rong)解，其(qi)(qi)(qi)濃(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)300 pg/ml，做系(xi)列倍(bei)(bei)比(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)，分(fen)(fen)別(bie)(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)直(zhi)接作(zuo)(zuo)為(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)度(du)(du)(du)0 pg/ml，臨(lin)(lin)用(yong)(yong)前(qian)(qian)(qian)(qian)(qian)(qian)(qian)15分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。 如(ru)(ru)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取0.5ml（不(bu)(bu)(bu)要少于(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)(shang)述(shu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)入含0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)中(zhong)，混(hun)勻(yun)(yun)(yun)(yun)即(ji)可(ke)(ke)(ke)(ke)(ke)，其(qi)(qi)(qi)余濃(nong)(nong)(nong)度(du)(du)(du)以(yi)(yi)(yi)(yi)(yi)(yi)此(ci)(ci)類推(tui)。2.生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)： 臨(lin)(lin)用(yong)(yong)前(qian)(qian)(qian)(qian)(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)(qian)(qian)(qian)根(gen)(gen)據(ju)預先(xian)(xian)計算(suan)(suan)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)驗(yan)所需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)100μl），實(shi)(shi)際(ji)(ji)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)多(duo)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)加(jia)(jia)(jia)990μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)勻(yun)(yun)(yun)(yun)，在(zai)(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)前(qian)(qian)(qian)(qian)(qian)(qian)(qian)一(yi)(yi)小(xiao)時(shi)(shi)(shi)(shi)(shi)內配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。3.辣(la)根(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)： 臨(lin)(lin)用(yong)(yong)前(qian)(qian)(qian)(qian)(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)辣(la)根(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)(qian)(qian)(qian)根(gen)(gen)據(ju)預先(xian)(xian)計算(suan)(suan)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)驗(yan)所需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)100μl），實(shi)(shi)際(ji)(ji)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)多(duo)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl辣(la)根(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)(su)(su)(su)加(jia)(jia)(jia)990μl辣(la)根(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)勻(yun)(yun)(yun)(yun)，在(zai)(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)前(qian)(qian)(qian)(qian)(qian)(qian)(qian)一(yi)(yi)小(xiao)時(shi)(shi)(shi)(shi)(shi)內配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。 操(cao)作(zuo)(zuo)步(bu)驟 實(shi)(shi)驗(yan)開始前(qian)(qian)(qian)(qian)(qian)(qian)(qian)，請(qing)(qing)提(ti)前(qian)(qian)(qian)(qian)(qian)(qian)(qian)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)置好所有(you)(you)(you)(you)(you)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)或(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)，均(jun)需(xu)(xu)混(hun)勻(yun)(yun)(yun)(yun)，混(hun)勻(yun)(yun)(yun)(yun)時(shi)(shi)(shi)(shi)(shi)盡(jin)量(liang)(liang)避(bi)(bi)免(mian)(mian)(mian)(mian)起泡(pao)。每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)該(gai)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)。如(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)過(guo)(guo)高(gao)時(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)進行(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)(yi)(yi)使(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合(he)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)范(fan)(fan)圍。 小(xiao)鼠(shu)(shu)脂肪酸(suan)結(jie)合(he)蛋(dan)白(bai)(bai)(bai)1(FABP1)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)三、操(cao)作(zuo)(zuo)步(bu)驟1.加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)別(bie)(bie)設空白(bai)(bai)(bai)孔(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)、待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)。空白(bai)(bai)(bai)孔(kong)(kong)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)分(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注意不(bu)(bu)(bu)要有(you)(you)(you)(you)(you)氣泡(pao)，加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)于(yu)(yu)(yu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)孔(kong)(kong)底(di)(di)部，盡(jin)量(liang)(liang)不(bu)(bu)(bu)觸(chu)及(ji)孔(kong)(kong)壁，輕(qing)輕(qing)晃動混(hun)勻(yun)(yun)(yun)(yun)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)加(jia)(jia)(jia)上(shang)(shang)(shang)(shang)蓋(gai)或(huo)(huo)(huo)(huo)覆膜(mo)，37℃反(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)120分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)保(bao)證實(shi)(shi)驗(yan)結(jie)果(guo)(guo)有(you)(you)(you)(you)(you)效(xiao)(xiao)性(xing)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)驗(yan)請(qing)(qing)使(shi)(shi)(shi)用(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。2.棄(qi)去液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干，不(bu)(bu)(bu)用(yong)(yong)洗(xi)滌(di)。每(mei)(mei)(mei)孔(kong)(kong)加(jia)(jia)(jia)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)加(jia)(jia)(jia)99μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)勻(yun)(yun)(yun)(yun)，在(zai)(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)前(qian)(qian)(qian)(qian)(qian)(qian)(qian)一(yi)(yi)小(xiao)時(shi)(shi)(shi)(shi)(shi)內配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。 3.溫育(yu)60分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)，棄(qi)去孔(kong)(kong)內液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干，洗(xi)板(ban)(ban)(ban)(ban)3次(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)浸泡(pao)1-2分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)，甩(shuai)干。 4.每(mei)(mei)(mei)孔(kong)(kong)加(jia)(jia)(jia)辣(la)根(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)(su)(su)(su)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同(tong)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。 5.溫育(yu)60分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)，棄(qi)去孔(kong)(kong)內液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干，洗(xi)板(ban)(ban)(ban)(ban)5次(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)浸泡(pao)1-2分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)，甩(shuai)干。 6.依(yi)序(xu)每(mei)(mei)(mei)孔(kong)(kong)加(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)(bi)光(guang)(guang)顯(xian)色(se)（30分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內，此(ci)(ci)時(shi)(shi)(shi)(shi)(shi)肉(rou)眼可(ke)(ke)(ke)(ke)(ke)見(jian)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)(qian)(qian)(qian)(qian)3-4孔(kong)(kong)有(you)(you)(you)(you)(you)明顯(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)藍色(se)，后(hou)(hou)(hou)3-4孔(kong)(kong)梯度(du)(du)(du)不(bu)(bu)(bu)明顯(xian)，即(ji)可(ke)(ke)(ke)(ke)(ke)終(zhong)止(zhi)）。 7.依(yi)序(xu)每(mei)(mei)(mei)孔(kong)(kong)加(jia)(jia)(jia)終(zhong)止(zhi)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止(zhi)反(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)（此(ci)(ci)時(shi)(shi)(shi)(shi)(shi)藍色(se)立(li)轉(zhuan)(zhuan)黃色(se)）。終(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)入順序(xu)應(ying)(ying)(ying)(ying)盡(jin)量(liang)(liang)與(yu)(yu)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)入順序(xu)相(xiang)(xiang)同(tong)。為(wei)(wei)(wei)了保(bao)證實(shi)(shi)驗(yan)結(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)(que)(que)性(xing)，底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)間到(dao)后(hou)(hou)(hou)應(ying)(ying)(ying)(ying)盡(jin)快加(jia)(jia)(jia)入終(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 8.用(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)聯(lian)儀在(zai)(zai)(zai)(zai)450nm波(bo)長依(yi)序(xu)測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)各孔(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)(guang)密度(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)）。 在(zai)(zai)(zai)(zai)加(jia)(jia)(jia)終(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)(yi)內進行(xing)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)。 四、試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)使(shi)(shi)(shi)用(yong)(yong)小(xiao)技巧1. 用(yong)(yong)戶在(zai)(zai)(zai)(zai)初次(ci)(ci)(ci)(ci)使(shi)(shi)(shi)用(yong)(yong)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)時(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)將(jiang)各種試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)管(guan)離(li)心數(shu)(shu)(shu)(shu)(shu)分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)集中(zhong)到(dao)管(guan)底(di)(di)。2. 每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)驗(yan)留(liu)一(yi)(yi)孔(kong)(kong)作(zuo)(zuo)為(wei)(wei)(wei)空白(bai)(bai)(bai)調零孔(kong)(kong)，該(gai)孔(kong)(kong)不(bu)(bu)(bu)加(jia)(jia)(jia)任何試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，只是**加(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)及(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)時(shi)(shi)(shi)(shi)(shi)先(xian)(xian)用(yong)(yong)此(ci)(ci)孔(kong)(kong)調OD值(zhi)(zhi)(zhi)(zhi)至零。 3. 為(wei)(wei)(wei)防止(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸(zheng)發，試(shi)(shi)(shi)(shi)驗(yan)時(shi)(shi)(shi)(shi)(shi)將(jiang)反(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)板(ban)(ban)(ban)(ban)放于(yu)(yu)(yu)鋪有(you)(you)(you)(you)(you)濕(shi)布(bu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉(bi)盒(he)(he)(he)(he)內，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)加(jia)(jia)(jia)上(shang)(shang)(shang)(shang)蓋(gai)或(huo)(huo)(huo)(huo)覆膜(mo)。 4. 未使(shi)(shi)(shi)用(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)或(huo)(huo)(huo)(huo)者試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，請(qing)(qing)于(yu)(yu)(yu)2-8℃保(bao)存(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)根(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)(su)(su)(su)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)請(qing)(qing)依(yi)據(ju)所需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)置使(shi)(shi)(shi)用(yong)(yong)。請(qing)(qing)勿(wu)重復使(shi)(shi)(shi)用(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)過(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)、辣(la)根(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)(su)(su)(su)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5. 建議(yi)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)均(jun)設雙(shuang)孔(kong)(kong)測(ce)(ce)(ce)(ce)(ce)定(ding)，以(yi)(yi)(yi)(yi)(yi)(yi)保(bao)證檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)(que)(que)性(xing)。 洗(xi)板(ban)(ban)(ban)(ban)方(fang)(fang)法(fa)(fa)(fa) 手工(gong)洗(xi)板(ban)(ban)(ban)(ban)方(fang)(fang)法(fa)(fa)(fa)：吸(xi)去（不(bu)(bu)(bu)可(ke)(ke)(ke)(ke)(ke)觸(chu)及(ji)板(ban)(ban)(ban)(ban)壁）或(huo)(huo)(huo)(huo)甩(shuai)掉酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)內的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)；在(zai)(zai)(zai)(zai)實(shi)(shi)驗(yan)臺上(shang)(shang)(shang)(shang)鋪墊(dian)幾層吸(xi)水紙(zhi)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)朝下(xia)(xia)用(yong)(yong)力拍(pai)幾次(ci)(ci)(ci)(ci)；將(jiang)推(tui)薦(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌(di)緩沖液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)至少0.3ml注入孔(kong)(kong)內，浸泡(pao)1-2分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。根(gen)(gen)據(ju)需(xu)(xu)要，重復此(ci)(ci)過(guo)(guo)程數(shu)(shu)(shu)(shu)(shu)次(ci)(ci)(ci)(ci)。 自動洗(xi)板(ban)(ban)(ban)(ban)：如(ru)(ru)果(guo)(guo)有(you)(you)(you)(you)(you)自動洗(xi)板(ban)(ban)(ban)(ban)機，應(ying)(ying)(ying)(ying)在(zai)(zai)(zai)(zai)熟練使(shi)(shi)(shi)用(yong)(yong)后(hou)(hou)(hou)再(zai)(zai)用(yong)(yong)到(dao)正式(shi)實(shi)(shi)驗(yan)過(guo)(guo)程中(zhong)。 計算(suan)(suan) 以(yi)(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)橫(heng)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對(dui)數(shu)(shu)(shu)(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)(zhi)為(wei)(wei)(wei)縱(zong)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)(zai)半對(dui)數(shu)(shu)(shu)(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)(shang)(shang)繪出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)，根(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)查出相(xiang)(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)；再(zai)(zai)乘以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)與(yu)(yu)OD值(zhi)(zhi)(zhi)(zhi)計算(suan)(suan)出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)(xian)回(hui)歸方(fang)(fang)程式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)代(dai)入方(fang)(fang)程式(shi)，計算(suan)(suan)出樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)，再(zai)(zai)乘以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)(shu)，即(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際(ji)(ji)濃(nong)(nong)(nong)度(du)(du)(du)。 五、技術(shu)小(xiao)提(ti)示(shi)1.當(dang)混(hun)合(he)蛋(dan)白(bai)(bai)(bai)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)盡(jin)量(liang)(liang)輕(qing)緩，避(bi)(bi)免(mian)(mian)(mian)(mian)起泡(pao)。2. 洗(xi)滌(di)過(guo)(guo)程非常重要，不(bu)(bu)(bu)充分(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌(di)易(yi)造成假陽性(xing)。 3. 一(yi)(yi)次(ci)(ci)(ci)(ci)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)間控制(zhi)(zhi)(zhi)(zhi)(zhi)在(zai)(zai)(zai)(zai)5分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)數(shu)(shu)(shu)(shu)(shu)量(liang)(liang)多(duo)，推(tui)薦(jian)使(shi)(shi)(shi)用(yong)(yong)排槍(qiang)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。 4. 請(qing)(qing)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi)(shi)(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)，做復孔(kong)(kong)。 5. 如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)中(zhong)待測(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)(liang)過(guo)(guo)高(gao)，請(qing)(qing)先(xian)(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)再(zai)(zai)測(ce)(ce)(ce)(ce)(ce)定(ding)，計算(suan)(suan)時(shi)(shi)(shi)(shi)(shi)請(qing)(qing)乘以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)(shu)。 6. 在(zai)(zai)(zai)(zai)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)，請(qing)(qing)以(yi)(yi)(yi)(yi)(yi)(yi)相(xiang)(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，不(bu)(bu)(bu)能(neng)混(hun)淆。 7. 底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)避(bi)(bi)光(guang)(guang)保(bao)存(cun)(cun)(cun)。 8. 不(bu)(bu)(bu)要用(yong)(yong)其(qi)(qi)(qi)它生(sheng)(sheng)產(chan)廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)替換試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)。 六、注意事項1.收集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)前(qian)(qian)(qian)(qian)(qian)(qian)(qian)必須(xu)清楚(chu)要檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成份是否(fou)足夠穩定(ding)。對(dui)收集后(hou)(hou)(hou)當(dang)天進行(xing)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)，儲存(cun)(cun)(cun)在(zai)(zai)(zai)(zai)4 ℃備用(yong)(yong)，如(ru)(ru)有(you)(you)(you)(you)(you)特(te)殊原(yuan)因(yin)需(xu)(xu)要周期收集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)， 將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)及(ji)時(shi)(shi)(shi)(shi)(shi)分(fen)(fen)裝后(hou)(hou)(hou)放在(zai)(zai)(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)-70 ℃條件(jian)下(xia)(xia)保(bao)存(cun)(cun)(cun)。避(bi)(bi)免(mian)(mian)(mian)(mian)反(fan)(fan)(fan)(fan)復凍融。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)(ke)(ke)(ke)保(bao)存(cun)(cun)(cun)48 小(xiao)時(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)(ke)(ke)(ke)保(bao)存(cun)(cun)(cun)1 個(ge)(ge)月(yue)。-70度(du)(du)(du)可(ke)(ke)(ke)(ke)(ke)保(bao)存(cun)(cun)(cun)6 個(ge)(ge)月(yue)。3.部分(fen)(fen)激素(su)(su)(su)(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)需(xu)(xu)添加(jia)(jia)(jia)抑肽酶(mei)(mei)(mei)(mei)(mei)。 4.計算(suan)(suan)方(fang)(fang)法(fa)(fa)(fa)：以(yi)(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)橫(heng)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)為(wei)(wei)(wei)縱(zong)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)(zai)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)(shang)(shang)繪出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)，根(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)查出相(xiang)(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)；再(zai)(zai)乘以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)與(yu)(yu)OD值(zhi)(zhi)(zhi)(zhi)計算(suan)(suan)出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)(xian)回(hui)歸方(fang)(fang)程式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)代(dai)入方(fang)(fang)程式(shi)，計算(suan)(suan)出樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)，再(zai)(zai)乘以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)(shu)，即(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際(ji)(ji)濃(nong)(nong)(nong)度(du)(du)(du)。 七(qi)、試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)性(xing)能(neng)1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)性(xing)回(hui)歸與(yu)(yu)預期濃(nong)(nong)(nong)度(du)(du)(du)相(xiang)(xiang)關系(xi)數(shu)(shu)(shu)(shu)(shu)R值(zhi)(zhi)(zhi)(zhi)為(wei)(wei)(wei)0.92以(yi)(yi)(yi)(yi)(yi)(yi)上(shang)(shang)(shang)(shang)。2.批內與(yu)(yu)批見(jian)應(ying)(ying)(ying)(ying)分(fen)(fen)別(bie)(bie)小(xiao)于(yu)(yu)(yu)9%和(he)(he)15% 八、保(bao)存(cun)(cun)(cun)條件(jian)及(ji)有(you)(you)(you)(you)(you)效(xiao)(xiao)期1.試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)保(bao)存(cun)(cun)(cun)：2-8℃。 2．有(you)(you)(you)(you)(you)效(xiao)(xiao)期：6個(ge)(ge)月(yue)

 小(xiao)(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)脂(zhi)(zhi)(zhi)(zhi)肪(fang)酸(suan)結(jie)(jie)(jie)合(he)蛋(dan)白(bai)(bai)1(FABP1)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)實(shi)驗(yan)(yan)原(yuan)理   試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)一(yi)(yi)(yi)(yi)(yi)步(bu)夾心(xin)法(fa)酶(mei)(mei)(mei)聯(lian)免疫吸附試(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)（ELISA）。往預(yu)(yu)先(xian)(xian)(xian)包被脂(zhi)(zhi)(zhi)(zhi)肪(fang)酸(suan)結(jie)(jie)(jie)合(he)蛋(dan)白(bai)(bai)1(FABP1)捕獲(huo)抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)(kong)(kong)(kong)(kong)中，依(yi)次(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)，經過(guo)(guo)(guo)溫(wen)育(yu)(yu)并徹底(di)(di)洗(xi)滌。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯色(se)(se)，TMB在(zai)(zai)過(guo)(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催(cui)化(hua)下轉化(hua)成藍色(se)(se)，并在(zai)(zai)酸(suan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)下轉化(hua)成＊終的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)(se)。顏(yan)色(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深(shen)淺(qian)和樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂(zhi)(zhi)(zhi)(zhi)肪(fang)酸(suan)結(jie)(jie)(jie)合(he)蛋(dan)白(bai)(bai)1(FABP1)呈(cheng)正(zheng)相關。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)450nm 波(bo)(bo)長下測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)吸光度(du)(du)(du)(du)(du)(du)(du)（OD 值(zhi)(zhi)(zhi)），計(ji)算樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。保(bao)存(cun)(cun)條(tiao)件(jian)及有(you)(you)效(xiao)期(qi)(qi)本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)體(ti)(ti)(ti)(ti)(ti)外定(ding)量(liang)(liang)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)血清(qing)、血漿、組織勻漿及相關液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)中脂(zhi)(zhi)(zhi)(zhi)肪(fang)酸(suan)結(jie)(jie)(jie)合(he)蛋(dan)白(bai)(bai)1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)。有(you)(you)效(xiao)期(qi)(qi)：6個月(yue)保(bao)存(cun)(cun)條(tiao)件(jian)：2-8℃小(xiao)(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)脂(zhi)(zhi)(zhi)(zhi)肪(fang)酸(suan)結(jie)(jie)(jie)合(he)蛋(dan)白(bai)(bai)1(FABP1)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)一(yi)(yi)(yi)(yi)(yi)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集及保(bao)存(cun)(cun) 1.血清(qing)：全血標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)請(qing)(qing)(qing)(qing)于(yu)(yu)室溫(wen)放置(zhi)(zhi)2小(xiao)(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)4℃過(guo)(guo)(guo)一(yi)(yi)(yi)(yi)(yi)晚后(hou)(hou)于(yu)(yu)1000 x g離(li)(li)心(xin)20分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，取上(shang)(shang)(shang)(shang)清(qing)即(ji)可(ke)(ke)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放于(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)存(cun)(cun)，但應(ying)(ying)(ying)避(bi)免反(fan)(fan)復(fu)凍融(rong)。 2.血漿：可(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)肝素(su)(su)(su)作(zuo)(zuo)為(wei)(wei)(wei)(wei)抗(kang)(kang)凝劑(ji)(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)采(cai)集后(hou)(hou)30分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)于(yu)(yu)2 - 8° C 1000 x g離(li)(li)心(xin)15分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放于(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)存(cun)(cun)，但應(ying)(ying)(ying)避(bi)免反(fan)(fan)復(fu)凍融(rong)。 3.細(xi)胞培(pei)養(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)(shang)清(qing)或(huo)(huo)(huo)其(qi)它(ta)生物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)：1000 x g離(li)(li)心(xin)20分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，取上(shang)(shang)(shang)(shang)清(qing)即(ji)可(ke)(ke)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放于(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)存(cun)(cun)，但應(ying)(ying)(ying)避(bi)免反(fan)(fan)復(fu)凍融(rong)。 注(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)溶(rong)(rong)血會影響檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)(jie)果(guo)(guo)，因(yin)此(ci)(ci)(ci)(ci)溶(rong)(rong)血標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)不(bu)(bu)(bu)(bu)宜(yi)進(jin)行此(ci)(ci)(ci)(ci)項(xiang)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。二、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)   首先(xian)(xian)(xian)通過(guo)(guo)(guo)文獻檢(jian)(jian)索(suo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)(fang)式(shi)了(le)解(jie)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大(da)致含量(liang)(liang)，確定(ding)適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)。只有(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范(fan)圍內(nei)(nei)(nei)，檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)(jie)(jie)果(guo)(guo)才(cai)是(shi)(shi)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)(cheng)中，應(ying)(ying)(ying)做(zuo)好詳細(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)錄(lu)。計(ji)算濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)了(le)“N”倍(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)應(ying)(ying)(ying)再(zai)乘(cheng)以(yi)(yi)(yi)(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：2瓶(ping)，每(mei)(mei)(mei)(mei)瓶(ping)臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)1ml，蓋好后(hou)(hou)靜置(zhi)(zhi)10分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)上(shang)(shang)(shang)(shang)，然后(hou)(hou)反(fan)(fan)復(fu)顛倒/搓動以(yi)(yi)(yi)(yi)助(zhu)溶(rong)(rong)解(jie)，其(qi)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)300 pg/ml，做(zuo)系(xi)(xi)列倍(bei)比(bi)(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)，分(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)直接作(zuo)(zuo)為(wei)(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)15分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)。 如(ru)配(pei)(pei)(pei)制(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取0.5ml（不(bu)(bu)(bu)(bu)要(yao)(yao)少于(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)含0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)中，混(hun)(hun)(hun)勻即(ji)可(ke)(ke)，其(qi)余濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)以(yi)(yi)(yi)(yi)此(ci)(ci)(ci)(ci)類推。2.生物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)： 臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)生物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)根(gen)(gen)據預(yu)(yu)先(xian)(xian)(xian)計(ji)算好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)驗(yan)(yan)所需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)配(pei)(pei)(pei)制(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際配(pei)(pei)(pei)制(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)(pei)制(zhi)(zhi)0.1-0.2ml。如(ru)10μl生物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)990μl生物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)例配(pei)(pei)(pei)制(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)(hun)勻，在(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)(yi)(yi)(yi)(yi)小(xiao)(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)。3.辣根(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和素(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)： 臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)辣根(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和素(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)根(gen)(gen)據預(yu)(yu)先(xian)(xian)(xian)計(ji)算好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)驗(yan)(yan)所需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)配(pei)(pei)(pei)制(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際配(pei)(pei)(pei)制(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)(pei)制(zhi)(zhi)0.1-0.2ml。如(ru)10μl辣根(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和素(su)(su)(su)加(jia)(jia)(jia)(jia)990μl辣根(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和素(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)例配(pei)(pei)(pei)制(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)(hun)勻，在(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)(yi)(yi)(yi)(yi)小(xiao)(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)。 操作(zuo)(zuo)步(bu)驟 實(shi)驗(yan)(yan)開始前(qian)(qian)，請(qing)(qing)(qing)(qing)提(ti)前(qian)(qian)配(pei)(pei)(pei)置(zhi)(zhi)好所有(you)(you)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)或(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，均(jun)需(xu)(xu)混(hun)(hun)(hun)勻，混(hun)(hun)(hun)勻時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盡(jin)量(liang)(liang)避(bi)免起泡。每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)該做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)。如(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)過(guo)(guo)(guo)高時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)進(jin)行稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)使(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)符(fu)合(he)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)圍。 小(xiao)(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)脂(zhi)(zhi)(zhi)(zhi)肪(fang)酸(suan)結(jie)(jie)(jie)合(he)蛋(dan)白(bai)(bai)1(FABP1)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)三、操作(zuo)(zuo)步(bu)驟1.加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)別(bie)設空(kong)(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)、待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)。空(kong)(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)分(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)意不(bu)(bu)(bu)(bu)要(yao)(yao)有(you)(you)氣泡，加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)于(yu)(yu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)底(di)(di)部，盡(jin)量(liang)(liang)不(bu)(bu)(bu)(bu)觸(chu)及孔(kong)(kong)(kong)(kong)(kong)壁，輕(qing)(qing)(qing)輕(qing)(qing)(qing)晃(huang)動混(hun)(hun)(hun)勻，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)上(shang)(shang)(shang)(shang)蓋或(huo)(huo)(huo)覆膜，37℃反(fan)(fan)應(ying)(ying)(ying)120分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)(wei)保(bao)證(zheng)實(shi)驗(yan)(yan)結(jie)(jie)(jie)果(guo)(guo)有(you)(you)效(xiao)性，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)驗(yan)(yan)請(qing)(qing)(qing)(qing)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)液(ye)(ye)(ye)(ye)。2.棄(qi)去(qu)液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩(shuai)干，不(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)滌。每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)生物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye) 100μl（取1μl生物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)99μl生物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)例配(pei)(pei)(pei)制(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)(hun)勻，在(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)(yi)(yi)(yi)(yi)小(xiao)(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)），37℃,60分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。 3.溫(wen)育(yu)(yu)60分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)，棄(qi)去(qu)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩(shuai)干，洗(xi)板(ban)(ban)(ban)(ban)3次(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)浸泡1-2分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)干。 4.每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)辣根(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和素(su)(su)(su)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)（同生物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。 5.溫(wen)育(yu)(yu)60分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)，棄(qi)去(qu)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩(shuai)干，洗(xi)板(ban)(ban)(ban)(ban)5次(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)浸泡1-2分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)干。 6.依(yi)序(xu)每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)90μl，37℃避(bi)光顯色(se)(se)（30分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)，此(ci)(ci)(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)肉眼可(ke)(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)有(you)(you)明顯的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯(ti)度(du)(du)(du)(du)(du)(du)(du)藍色(se)(se)，后(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)梯(ti)度(du)(du)(du)(du)(du)(du)(du)不(bu)(bu)(bu)(bu)明顯，即(ji)可(ke)(ke)終止）。 7.依(yi)序(xu)每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)終止溶(rong)(rong)液(ye)(ye)(ye)(ye)50μl，終止反(fan)(fan)應(ying)(ying)(ying)（此(ci)(ci)(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)藍色(se)(se)立轉黃色(se)(se)）。終止液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)順(shun)序(xu)應(ying)(ying)(ying)盡(jin)量(liang)(liang)與(yu)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)順(shun)序(xu)相同。為(wei)(wei)(wei)(wei)了(le)保(bao)證(zheng)實(shi)驗(yan)(yan)結(jie)(jie)(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性，底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)(fan)應(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)間到后(hou)(hou)應(ying)(ying)(ying)盡(jin)快加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)終止液(ye)(ye)(ye)(ye)。 8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)聯(lian)儀在(zai)(zai)450nm波(bo)(bo)長依(yi)序(xu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)各孔(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光密(mi)(mi)度(du)(du)(du)(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)）。 在(zai)(zai)加(jia)(jia)(jia)(jia)終止液(ye)(ye)(ye)(ye)后(hou)(hou)15分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)內(nei)(nei)(nei)進(jin)行檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。 四、試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)小(xiao)(xiao)(xiao)(xiao)技巧1. 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)初次(ci)(ci)(ci)(ci)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)將(jiang)(jiang)各種試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)管(guan)離(li)(li)心(xin)數(shu)(shu)(shu)分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)集中到管(guan)底(di)(di)。2. 每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)驗(yan)(yan)留一(yi)(yi)(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)為(wei)(wei)(wei)(wei)空(kong)(kong)白(bai)(bai)調(diao)零(ling)(ling)孔(kong)(kong)(kong)(kong)(kong)，該孔(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)(bu)加(jia)(jia)(jia)(jia)任何試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，只是(shi)(shi)**加(jia)(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)及2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)先(xian)(xian)(xian)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)此(ci)(ci)(ci)(ci)孔(kong)(kong)(kong)(kong)(kong)調(diao)OD值(zhi)(zhi)(zhi)至(zhi)零(ling)(ling)。 3. 為(wei)(wei)(wei)(wei)防止樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發(fa)，試(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)反(fan)(fan)應(ying)(ying)(ying)板(ban)(ban)(ban)(ban)放于(yu)(yu)鋪(pu)有(you)(you)濕(shi)布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密(mi)(mi)閉盒(he)(he)(he)內(nei)(nei)(nei)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)上(shang)(shang)(shang)(shang)蓋或(huo)(huo)(huo)覆膜。 4. 未使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)或(huo)(huo)(huo)者試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，請(qing)(qing)(qing)(qing)于(yu)(yu)2-8℃保(bao)存(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)、辣根(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和素(su)(su)(su)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)請(qing)(qing)(qing)(qing)依(yi)據所需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)配(pei)(pei)(pei)置(zhi)(zhi)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)(qing)(qing)勿重(zhong)復(fu)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)或(huo)(huo)(huo)、辣根(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和素(su)(su)(su)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)。 5. 建議檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)均(jun)設雙孔(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)，以(yi)(yi)(yi)(yi)保(bao)證(zheng)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性。 洗(xi)板(ban)(ban)(ban)(ban)方(fang)(fang)(fang)法(fa) 手工(gong)洗(xi)板(ban)(ban)(ban)(ban)方(fang)(fang)(fang)法(fa)：吸去(qu)（不(bu)(bu)(bu)(bu)可(ke)(ke)觸(chu)及板(ban)(ban)(ban)(ban)壁）或(huo)(huo)(huo)甩(shuai)掉酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)內(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)；在(zai)(zai)實(shi)驗(yan)(yan)臺上(shang)(shang)(shang)(shang)鋪(pu)墊幾層吸水(shui)紙，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)朝下用(yong)(yong)(yong)(yong)(yong)(yong)(yong)力(li)拍幾次(ci)(ci)(ci)(ci)；將(jiang)(jiang)推薦(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌緩沖液(ye)(ye)(ye)(ye)至(zhi)少0.3ml注(zhu)入(ru)(ru)(ru)(ru)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)，浸泡1-2分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。根(gen)(gen)據需(xu)(xu)要(yao)(yao)，重(zhong)復(fu)此(ci)(ci)(ci)(ci)過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)(cheng)數(shu)(shu)(shu)次(ci)(ci)(ci)(ci)。 自(zi)動洗(xi)板(ban)(ban)(ban)(ban)：如(ru)果(guo)(guo)有(you)(you)自(zi)動洗(xi)板(ban)(ban)(ban)(ban)機，應(ying)(ying)(ying)在(zai)(zai)熟(shu)練使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)再(zai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)到正(zheng)式(shi)實(shi)驗(yan)(yan)過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)(cheng)中。 計(ji)算 以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)半對數(shu)(shu)(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)(shang)繪(hui)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)，根(gen)(gen)據樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)查出(chu)相應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)；再(zai)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)算出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)回(hui)歸方(fang)(fang)(fang)程(cheng)(cheng)(cheng)(cheng)(cheng)式(shi)，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)(ru)(ru)(ru)方(fang)(fang)(fang)程(cheng)(cheng)(cheng)(cheng)(cheng)式(shi)，計(ji)算出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)，再(zai)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)，即(ji)為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。 五(wu)、技術(shu)小(xiao)(xiao)(xiao)(xiao)提(ti)示1.當混(hun)(hun)(hun)合(he)蛋(dan)白(bai)(bai)溶(rong)(rong)液(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)盡(jin)量(liang)(liang)輕(qing)(qing)(qing)緩，避(bi)免起泡。2. 洗(xi)滌過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)(cheng)非(fei)常(chang)重(zhong)要(yao)(yao)，不(bu)(bu)(bu)(bu)充分(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌易造(zao)成假(jia)陽(yang)性。 3. 一(yi)(yi)(yi)(yi)(yi)次(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)間控制(zhi)(zhi)在(zai)(zai)5分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)(shu)(shu)量(liang)(liang)多，推薦(jian)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)。 4. 請(qing)(qing)(qing)(qing)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)，做(zuo)復(fu)孔(kong)(kong)(kong)(kong)(kong)。 5. 如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)(liang)過(guo)(guo)(guo)高，請(qing)(qing)(qing)(qing)先(xian)(xian)(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)再(zai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)，計(ji)算時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)請(qing)(qing)(qing)(qing)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)。 6. 在(zai)(zai)配(pei)(pei)(pei)制(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)液(ye)(ye)(ye)(ye)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)(qing)(qing)(qing)以(yi)(yi)(yi)(yi)相應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)配(pei)(pei)(pei)制(zhi)(zhi)，不(bu)(bu)(bu)(bu)能混(hun)(hun)(hun)淆。 7. 底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)(qing)(qing)避(bi)光保(bao)存(cun)(cun)。 8. 不(bu)(bu)(bu)(bu)要(yao)(yao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)其(qi)它(ta)生產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)替換試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)中的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)。 六(liu)、注(zhu)意事(shi)項(xiang)1.收(shou)集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)前(qian)(qian)必(bi)須清(qing)楚要(yao)(yao)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成份是(shi)(shi)否足夠穩定(ding)。對收(shou)集后(hou)(hou)當天(tian)進(jin)行檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)，儲存(cun)(cun)在(zai)(zai)4 ℃備(bei)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)有(you)(you)特殊原(yuan)因(yin)需(xu)(xu)要(yao)(yao)周(zhou)期(qi)(qi)收(shou)集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)， 將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)及時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)裝后(hou)(hou)放在(zai)(zai)-20 ℃或(huo)(huo)(huo)-70 ℃條(tiao)件(jian)下保(bao)存(cun)(cun)。避(bi)免反(fan)(fan)復(fu)凍融(rong)。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)保(bao)存(cun)(cun)48 小(xiao)(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)保(bao)存(cun)(cun)1 個月(yue)。-70度(du)(du)(du)(du)(du)(du)(du)可(ke)(ke)保(bao)存(cun)(cun)6 個月(yue)。3.部分(fen)(fen)激素(su)(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)需(xu)(xu)添加(jia)(jia)(jia)(jia)抑肽酶(mei)(mei)(mei)。 4.計(ji)算方(fang)(fang)(fang)法(fa)：以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)(shang)繪(hui)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)，根(gen)(gen)據樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)查出(chu)相應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)；再(zai)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)算出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)回(hui)歸方(fang)(fang)(fang)程(cheng)(cheng)(cheng)(cheng)(cheng)式(shi)，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)(ru)(ru)(ru)方(fang)(fang)(fang)程(cheng)(cheng)(cheng)(cheng)(cheng)式(shi)，計(ji)算出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)，再(zai)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)，即(ji)為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。 七、試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)性能1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)(xian)性回(hui)歸與(yu)預(yu)(yu)期(qi)(qi)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)相關系(xi)(xi)數(shu)(shu)(shu)R值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)0.92以(yi)(yi)(yi)(yi)上(shang)(shang)(shang)(shang)。2.批內(nei)(nei)(nei)與(yu)批見應(ying)(ying)(ying)分(fen)(fen)別(bie)小(xiao)(xiao)(xiao)(xiao)于(yu)(yu)9%和15% 八、保(bao)存(cun)(cun)條(tiao)件(jian)及有(you)(you)效(xiao)期(qi)(qi)1.試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)保(bao)存(cun)(cun)：2-8℃。 2．有(you)(you)效(xiao)期(qi)(qi)：6個月(yue)

3-磷酸(suan)(suan)甘(gan)(gan)油酸(suan)(suan)激酶(mei)(mei)(mei)(mei)(PGK)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)科(ke)研實(shi)驗,不(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)臨床。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)可(ke)(ke)以(yi)(yi)(yi)(yi)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)型(xing)(xing)(xing)有(you)(you)(you)(you)(you)哪(na)些(xie)(xie)???生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗原(yuan)(yuan)理(li)(li)(li)(li)(li)(li)(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)基(ji)于(yu)(yu)(yu)(yu)化(hua)(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)反(fan)(fan)(fan)(fan)應(ying)(ying),理(li)(li)(li)(li)(li)(li)(li)(li)論上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)受(shou)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)種(zhong)屬(shu)(shu)(shu)及(ji)(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)型(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)制(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通常(chang)(chang)(chang)只(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)型(xing)(xing)(xing),不(bu)(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)種(zhong)屬(shu)(shu)(shu)。但(dan)(dan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)某(mou)些(xie)(xie)指(zhi)(zhi)(zhi)標(biao)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)不(bu)(bu)(bu)(bu)同(tong)(tong)種(zhong)屬(shu)(shu)(shu)或(huo)(huo)(huo)(huo)(huo)類(lei)(lei)型(xing)(xing)(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方(fang)(fang)式(shi)不(bu)(bu)(bu)(bu)同(tong)(tong)。因(yin)(yin)此(ci)(ci),具體(ti)(ti)(ti)(ti)(ti)(ti)(ti)情(qing)況(kuang)請參見(jian)(jian)說(shuo)(shuo)(shuo)(shuo)明(ming)(ming)書(shu)或(huo)(huo)(huo)(huo)(huo)與(yu)技(ji)(ji)術(shu)支持(chi)聯系(xi)(xi)確(que)(que)(que)認。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)般(ban)(ban)要(yao)(yao)(yao)(yao)(yao)(yao)求(qiu):1)于(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)無溶(rong)血(xue)(xue)(xue)、脂(zhi)(zhi)血(xue)(xue)(xue)、的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現象(xiang)。組(zu)織樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)將(jiang)(jiang)血(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)洗凈擦干(gan)(gan)后(hou)再進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)稱重(zhong)(zhong)(zhong)(zhong)(zhong)處理(li)(li)(li)(li)(li)(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)處理(li)(li)(li)(li)(li)(li)(li)(li)后(hou)盡(jin)快(kuai)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且(qie)(qie)保(bao)(bao)存(cun)(cun)(cun)于(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)盒(he)(he)(he)(he)(he)(he)(he)(he)之(zhi)(zhi)(zhi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang),待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。求(qiu)3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing),若不(bu)(bu)(bu)(bu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing),需(xu)(xu)(xu)(xu)離(li)(li)(li)心(xin)(xin)(xin)(xin)后(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特殊指(zhi)(zhi)(zhi)標(biao)除(chu)外(wai)(wai)(wai)(wai))。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)值應(ying)(ying)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)線性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)(fan)圍(wei)內,若超(chao)(chao)過(guo)(guo)(guo)線性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)(fan)圍(wei),需(xu)(xu)(xu)(xu)稀(xi)釋后(hou)再進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若低(di)(di)于(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)(fan)(fan)圍(wei),需(xu)(xu)(xu)(xu)增(zeng)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)濃度(du)(du)后(hou)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)(fa)(fa)(fa)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)度(du)(du)法(fa)(fa)(fa)(fa)(fa)(fa)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie)?分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)度(du)(du)法(fa)(fa)(fa)(fa)(fa)(fa):主(zhu)流(liu)(liu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)為(wei)(wei)(wei)(wei)(wei)1mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)器(qi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)度(du)(du)計(ji)(ji)(ji)(ji)(ji)(ji),能(neng)(neng)夠(gou)大(da)(da)(da)(da)限(xian)度(du)(du)地滿足絕大(da)(da)(da)(da)多數(shu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)(hu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)。微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)(fa)(fa)(fa):主(zhu)流(liu)(liu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)為(wei)(wei)(wei)(wei)(wei)0.2mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)器(qi)或(huo)(huo)(huo)(huo)(huo)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)度(du)(du)計(ji)(ji)(ji)(ji)(ji)(ji),測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)更(geng)(geng)加(jia)(jia)(jia)(jia)簡(jian)便快(kuai)捷。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)規(gui)格(ge)(ge)(ge)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)100管(guan)(guan)(guan)(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)50管(guan)(guan)(guan)(guan)(guan)(guan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)什么意(yi)(yi)(yi)(yi)思?(1)50管(guan)(guan)(guan)(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):50管(guan)(guan)(guan)(guan)(guan)(guan)指(zhi)(zhi)(zhi)能(neng)(neng)夠(gou)做(zuo)50次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)(zhi)(zhi)可(ke)(ke)以(yi)(yi)(yi)(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)48個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)。如(ru)(ru)(ru)(ru)果(guo)每(mei)個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)重(zhong)(zhong)(zhong)(zhong)(zhong)復(fu)(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci)(ci)(ci),那(nei)么只(zhi)(zhi)能(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)16個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)。其(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)(you)(you)2次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)空白管(guan)(guan)(guan)(guan)(guan)(guan)或(huo)(huo)(huo)(huo)(huo)者(zhe)(zhe)對(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)。值得(de)(de)特別(bie)注(zhu)意(yi)(yi)(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi),50管(guan)(guan)(guan)(guan)(guan)(guan)/24樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由(you)于(yu)(yu)(yu)(yu)每(mei)個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)做(zuo)對(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)(zhao),因(yin)(yin)此(ci)(ci)能(neng)(neng)夠(gou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)僅(jin)為(wei)(wei)(wei)(wei)(wei)8個(ge)(ge)(ge)(ge)(假設做(zuo)3次(ci)(ci)(ci)(ci)重(zhong)(zhong)(zhong)(zhong)(zhong)復(fu)(fu))(2)100管(guan)(guan)(guan)(guan)(guan)(guan)/96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):100管(guan)(guan)(guan)(guan)(guan)(guan)指(zhi)(zhi)(zhi)能(neng)(neng)夠(gou)做(zuo)100次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)(zhi)(zhi)可(ke)(ke)以(yi)(yi)(yi)(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)96個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin),如(ru)(ru)(ru)(ru)果(guo)每(mei)個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)重(zhong)(zhong)(zhong)(zhong)(zhong)復(fu)(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci)(ci)(ci),那(nei)么只(zhi)(zhi)能(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)32個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)。其(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)(you)(you)4次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)空白管(guan)(guan)(guan)(guan)(guan)(guan)或(huo)(huo)(huo)(huo)(huo)者(zhe)(zhe)對(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)。注(zhu)意(yi)(yi)(yi)(yi)由(you)于(yu)(yu)(yu)(yu)瓶(ping)(ping)璧和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)移液(ye)(ye)(ye)(ye)(ye)(ye)器(qi)吸(xi)附等(deng),通常(chang)(chang)(chang)要(yao)(yao)(yao)(yao)(yao)(yao)比(bi)(bi)(bi)理(li)(li)(li)(li)(li)(li)(li)(li)論測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)次(ci)(ci)(ci)(ci)數(shu)少(shao)1~3次(ci)(ci)(ci)(ci)。其(qi)它規(gui)格(ge)(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)(han)(han)(han)義依(yi)次(ci)(ci)(ci)(ci)類(lei)(lei)推。值得(de)(de)特別(bie)注(zhu)意(yi)(yi)(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi),100管(guan)(guan)(guan)(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由(you)于(yu)(yu)(yu)(yu)每(mei)個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)做(zuo)對(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)(zhao),因(yin)(yin)此(ci)(ci)能(neng)(neng)夠(gou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)僅(jin)為(wei)(wei)(wei)(wei)(wei)16個(ge)(ge)(ge)(ge)(假設做(zuo)3次(ci)(ci)(ci)(ci)重(zhong)(zhong)(zhong)(zhong)(zhong)復(fu)(fu))。如(ru)(ru)(ru)(ru)何防(fang)(fang)止(zhi)(zhi)(zhi)(zhi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)失(shi)(shi)效?????不(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)(yao)(yao)過(guo)(guo)(guo)早(zao)訂(ding)購(gou)(gou),以(yi)(yi)(yi)(yi)免試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)失(shi)(shi)效.收(shou)到試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)后(hou),檢(jian)(jian)(jian)查外(wai)(wai)(wai)(wai)包(bao)裝(zhuang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)正常(chang)(chang)(chang)。馬上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)打(da)開,按(an)照(zhao)(zhao)(zhao)(zhao)(zhao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)說(shuo)(shuo)(shuo)(shuo)明(ming)(ming)書(shu)核(he)對(dui)(dui)(dui)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)正確(que)(que)(que),注(zhu)意(yi)(yi)(yi)(yi)瓶(ping)(ping)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)狀(zhuang)(zhuang)態(tai)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)與(yu)說(shuo)(shuo)(shuo)(shuo)明(ming)(ming)書(shu)一(yi)(yi)致。如(ru)(ru)(ru)(ru)果(guo)有(you)(you)(you)(you)(you)問題,或(huo)(huo)(huo)(huo)(huo)者(zhe)(zhe)懷疑,馬上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)聯系(xi)(xi)公司(si)(si)銷售,進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)確(que)(que)(que)認。如(ru)(ru)(ru)(ru)果(guo)沒有(you)(you)(you)(you)(you)問題,請務(wu)必(bi)按(an)照(zhao)(zhao)(zhao)(zhao)(zhao)說(shuo)(shuo)(shuo)(shuo)明(ming)(ming)書(shu)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)存(cun)(cun)(cun)。注(zhu)意(yi)(yi)(yi)(yi),不(bu)(bu)(bu)(bu)同(tong)(tong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji),可(ke)(ke)能(neng)(neng)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)保(bao)(bao)存(cun)(cun)(cun)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)常(chang)(chang)(chang)溫(25°℃)、4℃和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)-20℃,務(wu)必(bi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)按(an)照(zhao)(zhao)(zhao)(zhao)(zhao)要(yao)(yao)(yao)(yao)(yao)(yao)求(qiu)保(bao)(bao)存(cun)(cun)(cun),不(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)(yao)(yao)整(zheng)個(ge)(ge)(ge)(ge)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)放置(zhi)(zhi)于(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)箱。如(ru)(ru)(ru)(ru)何進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)?(1)務(wu)必(bi)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)7個(ge)(ge)(ge)(ge)工作(zuo)(zuo)(zuo)日內進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),以(yi)(yi)(yi)(yi)確(que)(que)(que)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)該試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)適合(he)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin),以(yi)(yi)(yi)(yi)免造成(cheng)(cheng)(cheng)(cheng)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浪費(fei)。(2)選(xuan)擇(ze)2~3個(ge)(ge)(ge)(ge)預(yu)(yu)期差(cha)異(yi)(yi)比(bi)(bi)(bi)較大(da)(da)(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin),嚴格(ge)(ge)(ge)按(an)照(zhao)(zhao)(zhao)(zhao)(zhao)說(shuo)(shuo)(shuo)(shuo)明(ming)(ming)書(shu)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)操作(zuo)(zuo)(zuo),注(zhu)意(yi)(yi)(yi)(yi)儀(yi)器(qi)工作(zuo)(zuo)(zuo)狀(zhuang)(zhuang)態(tai),操作(zuo)(zuo)(zuo)規(gui)范(fan)(fan)(fan),控制(zhi)好測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)條件(jian),尤(you)其(qi)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)溫度(du)(du)。(3)如(ru)(ru)(ru)(ru)實(shi)與(yu)公司(si)(si)銷售溝(gou)通預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)(jie)果(guo),公司(si)(si)技(ji)(ji)術(shu)人員會確(que)(que)(que)認測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)(jie)果(guo)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)正常(chang)(chang)(chang),是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)調整(zheng),如(ru)(ru)(ru)(ru)何進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)調整(zheng),從(cong)而(er)確(que)(que)(que)保(bao)(bao)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)(jie)果(guo)可(ke)(ke)靠(kao)(kao)。熒(ying)(ying)(ying)(ying) 光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang) 分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen) 析(xi)(xi)(xi)(xi)(xi)(xi)(xi) 法(fa)(fa)(fa)(fa)(fa)(fa)?????????熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)法(fa)(fa)(fa)(fa)(fa)(fa)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)指(zhi)(zhi)(zhi)利用(yong)(yong)(yong)(yong)(yong)(yong)(yong)某(mou)些(xie)(xie)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)被(bei)紫(zi)外(wai)(wai)(wai)(wai)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)照(zhao)(zhao)(zhao)(zhao)(zhao)射(she)后(hou)處于(yu)(yu)(yu)(yu)激發(fa)(fa)(fa)態(tai)，激發(fa)(fa)(fa)態(tai)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)經歷一(yi)(yi)個(ge)(ge)(ge)(ge)碰撞(zhuang)及(ji)(ji)(ji)發(fa)(fa)(fa)射(she)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)去激發(fa)(fa)(fa)過(guo)(guo)(guo)程所(suo)(suo)發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)能(neng)(neng)反(fan)(fan)(fan)(fan)映出(chu)該物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)特性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)，可(ke)(ke)以(yi)(yi)(yi)(yi)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)或(huo)(huo)(huo)(huo)(huo)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)法(fa)(fa)(fa)(fa)(fa)(fa)。由(you)于(yu)(yu)(yu)(yu)有(you)(you)(you)(you)(you)些(xie)(xie)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)身不(bu)(bu)(bu)(bu)發(fa)(fa)(fa)射(she)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)（或(huo)(huo)(huo)(huo)(huo)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)很(hen)弱），這(zhe)就需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)把(ba)不(bu)(bu)(bu)(bu)發(fa)(fa)(fa)射(she)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)轉(zhuan)化(hua)(hua)(hua)(hua)(hua)(hua)成(cheng)(cheng)(cheng)(cheng)能(neng)(neng)發(fa)(fa)(fa)射(she)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。例(li)(li)如(ru)(ru)(ru)(ru)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)某(mou)些(xie)(xie)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)（如(ru)(ru)(ru)(ru)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)染料），使(shi)(shi)其(qi)與(yu)不(bu)(bu)(bu)(bu)發(fa)(fa)(fa)射(she)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng)(cheng)絡(luo)合(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)，各(ge)種(zhong)絡(luo)合(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)能(neng)(neng)發(fa)(fa)(fa)射(she)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)，再進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)。因(yin)(yin)此(ci)(ci)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)，對(dui)(dui)(dui)一(yi)(yi)些(xie)(xie)原(yuan)(yuan)來(lai)不(bu)(bu)(bu)(bu)發(fa)(fa)(fa)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)無機(ji)(ji)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)有(you)(you)(you)(you)(you)機(ji)(ji)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)打(da)開了(le)(le)大(da)(da)(da)(da)門，擴展了(le)(le)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范(fan)(fan)(fan)圍(wei)。比(bi)(bi)(bi)色(se)皿(min)(min)(min)(min)(min)(min)有(you)(you)(you)(you)(you)哪(na)些(xie)(xie)規(gui)格(ge)(ge)(ge)?(1)常(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)比(bi)(bi)(bi)色(se)皿(min)(min)(min)(min)(min)(min),如(ru)(ru)(ru)(ru)1mL和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)3mL等(deng),常(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)(fa)(fa)(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)都(dou)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)選(xuan)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)1mL體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi),只(zhi)(zhi)能(neng)(neng)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)1mL比(bi)(bi)(bi)色(se)皿(min)(min)(min)(min)(min)(min),不(bu)(bu)(bu)(bu)可(ke)(ke)以(yi)(yi)(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)更(geng)(geng)大(da)(da)(da)(da)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)色(se)皿(min)(min)(min)(min)(min)(min),否(fou)則(ze)(ze)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)夠(gou),無法(fa)(fa)(fa)(fa)(fa)(fa)正常(chang)(chang)(chang)比(bi)(bi)(bi)色(se)。(2)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)比(bi)(bi)(bi)色(se)皿(min)(min)(min)(min)(min)(min),如(ru)(ru)(ru)(ru)0.25mL和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)0.5mL等(deng),微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)(fa)(fa)(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)都(dou)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)選(xuan)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)0.25mL比(bi)(bi)(bi)色(se)皿(min)(min)(min)(min)(min)(min)。一(yi)(yi)般(ban)(ban)客戶(hu)(hu)都(dou)選(xuan)擇(ze)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)儀(yi)(需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)自備酶(mei)(mei)(mei)(mei)標(biao)板(ban))(3)紫(zi)外(wai)(wai)(wai)(wai)波(bo)長(chang)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)石(shi)英比(bi)(bi)(bi)色(se)皿(min)(min)(min)(min)(min)(min),不(bu)(bu)(bu)(bu)可(ke)(ke)以(yi)(yi)(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)普通玻璃比(bi)(bi)(bi)色(se)皿(min)(min)(min)(min)(min)(min)。(4)不(bu)(bu)(bu)(bu)同(tong)(tong)規(gui)格(ge)(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)色(se)皿(min)(min)(min)(min)(min)(min),其(qi)外(wai)(wai)(wai)(wai)觀大(da)(da)(da)(da)小(xiao)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)致的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de),都(dou)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)1cm光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)徑(也有(you)(you)(you)(you)(you)0.5cm光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)徑的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)色(se)皿(min)(min)(min)(min)(min)(min),不(bu)(bu)(bu)(bu)常(chang)(chang)(chang)見(jian)(jian),也可(ke)(ke)以(yi)(yi)(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong),但(dan)(dan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)時(shi)(shi)(shi)(shi)要(yao)(yao)(yao)(yao)(yao)(yao)注(zhu)意(yi)(yi)(yi)(yi)修改計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)公式(shi)),其(qi)內容積(ji)差(cha)異(yi)(yi)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)于(yu)(yu)(yu)(yu)壁的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)厚度(du)(du)。代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)優(you)點(1)更(geng)(geng)加(jia)(jia)(jia)(jia)省(sheng)事兒,完(wan)(wan)全(quan)(quan)不(bu)(bu)(bu)(bu)必(bi)為(wei)(wei)(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)犯難。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)(hu)只(zhi)(zhi)要(yao)(yao)(yao)(yao)(yao)(yao)提(ti)(ti)(ti)(ti)(ti)(ti)供樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)想測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)指(zhi)(zhi)(zhi)標(biao),就不(bu)(bu)(bu)(bu)再需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)為(wei)(wei)(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)操心(xin)(xin)(xin)(xin)了(le)(le),不(bu)(bu)(bu)(bu)再受(shou)到操作(zuo)(zuo)(zuo)技(ji)(ji)能(neng)(neng)、儀(yi)器(qi)設備和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)時(shi)(shi)(shi)(shi)間的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)制(zhi),就等(deng)著拿(na)到數(shu)據寫論文。(2)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)數(shu)據更(geng)(geng)有(you)(you)(you)(you)(you)保(bao)(bao)障。嚴格(ge)(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)管(guan)(guan)(guan)(guan)(guan)(guan)理(li)(li)(li)(li)(li)(li)(li)(li)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi),從(cong)而(er)保(bao)(bao)證(zheng)(zheng)了(le)(le)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)真實(shi)可(ke)(ke)靠(kao)(kao)。我們還(huan)(huan)建(jian)(jian)(jian)(jian)立(li)了(le)(le)免費(fei)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)制(zhi)度(du)(du)。(3)防(fang)(fang)止(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)損失(shi)(shi)無論是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)(hu)自己完(wan)(wan)成(cheng)(cheng)(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)全(quan)(quan)過(guo)(guo)(guo)程,還(huan)(huan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)購(gou)(gou)買試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)完(wan)(wan)成(cheng)(cheng)(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),都(dou)可(ke)(ke)能(neng)(neng)因(yin)(yin)為(wei)(wei)(wei)(wei)(wei)缺(que)乏測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)經驗,導致測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)失(shi)(shi)敗,從(cong)而(er)損失(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情(qing)況(kuang)。對(dui)(dui)(dui)于(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)命科(ke)學(xue)(xue)(xue)(xue)研究(jiu)(jiu)而(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)＊珍(zhen)貴的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)一(yi)(yi)旦(dan)損失(shi)(shi),輕則(ze)(ze)造成(cheng)(cheng)(cheng)(cheng)研究(jiu)(jiu)進(jin)(jin)(jin)(jin)(jin)度(du)(du)被(bei)耽擱,重(zhong)(zhong)(zhong)(zhong)(zhong)則(ze)(ze)造成(cheng)(cheng)(cheng)(cheng)研究(jiu)(jiu)完(wan)(wan)全(quan)(quan)失(shi)(shi)敗!代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)能(neng)(neng)夠(gou)充(chong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)保(bao)(bao)證(zheng)(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)(cheng)(cheng)功(gong),從(cong)而(er)防(fang)(fang)止(zhi)(zhi)(zhi)(zhi)了(le)(le)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)損失(shi)(shi)。當然,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)(cheng)(cheng)功(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前提(ti)(ti)(ti)(ti)(ti)(ti),而(er)且(qie)(qie)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)只(zhi)(zhi)有(you)(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)(hu)自己才能(neng)(neng)控制(zhi)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)保(bao)(bao)證(zheng)(zheng)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)原(yuan)(yuan)理(li)(li)(li)(li)(li)(li)(li)(li)??生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he),又(you)稱生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he),是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)利用(yong)(yong)(yong)(yong)(yong)(yong)(yong)特定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)、動(dong)力學(xue)(xue)(xue)(xue)及(ji)(ji)(ji)化(hua)(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)反(fan)(fan)(fan)(fan)應(ying)(ying)等(deng)技(ji)(ji)術(shu)制(zhi)備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he),用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)(li)(li)(li)(li)(li)(li)(li)學(xue)(xue)(xue)(xue)、病理(li)(li)(li)(li)(li)(li)(li)(li)學(xue)(xue)(xue)(xue)、藥理(li)(li)(li)(li)(li)(li)(li)(li)學(xue)(xue)(xue)(xue)等(deng)領域中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)特定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)或(huo)(huo)(huo)(huo)(huo)化(hua)(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)包(bao)括底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、酶(mei)(mei)(mei)(mei)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)、探針等(deng)多個(ge)(ge)(ge)(ge)組(zu)成(cheng)(cheng)(cheng)(cheng)部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen),其(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)＊常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)(mei)法(fa)(fa)(fa)(fa)(fa)(fa)。底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)(mei)法(fa)(fa)(fa)(fa)(fa)(fa)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)原(yuan)(yuan)理(li)(li)(li)(li)(li)(li)(li)(li)。在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)該過(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)與(yu)酶(mei)(mei)(mei)(mei)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)細(xi)(xi)(xi)胞(bao)(bao)膜上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)結(jie)(jie)合(he)形(xing)成(cheng)(cheng)(cheng)(cheng)基(ji)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)酶(mei)(mei)(mei)(mei),進(jin)(jin)(jin)(jin)(jin)而(er)形(xing)成(cheng)(cheng)(cheng)(cheng)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)(mei)復(fu)(fu)合(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)。隨(sui)著時(shi)(shi)(shi)(shi)間的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)推移,底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)(mei)復(fu)(fu)合(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)逐漸(jian)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)解(jie)(jie)(jie),釋放出(chu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)小(xiao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)大(da)(da)(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)未完(wan)(wan)全(quan)(quan)消(xiao)化(hua)(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)。未完(wan)(wan)全(quan)(quan)消(xiao)化(hua)(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)可(ke)(ke)以(yi)(yi)(yi)(yi)被(bei)一(yi)(yi)系(xi)(xi)列的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)代(dai)謝,同(tong)(tong)時(shi)(shi)(shi)(shi)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、酶(mei)(mei)(mei)(mei)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)代(dai)謝產(chan)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)都(dou)會對(dui)(dui)(dui)復(fu)(fu)合(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)穩定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)產(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)影響,從(cong)而(er)影響檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)果(guo)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)可(ke)(ke)以(yi)(yi)(yi)(yi)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)型(xing)(xing)(xing)有(you)(you)(you)(you)(you)哪(na)些(xie)(xie)?? ?生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗原(yuan)(yuan)理(li)(li)(li)(li)(li)(li)(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)基(ji)于(yu)(yu)(yu)(yu)化(hua)(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)反(fan)(fan)(fan)(fan)應(ying)(ying),理(li)(li)(li)(li)(li)(li)(li)(li)論上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)受(shou)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)種(zhong)屬(shu)(shu)(shu)及(ji)(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)型(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)制(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通常(chang)(chang)(chang)只(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)型(xing)(xing)(xing),不(bu)(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)種(zhong)屬(shu)(shu)(shu)。但(dan)(dan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)某(mou)些(xie)(xie)指(zhi)(zhi)(zhi)標(biao)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)不(bu)(bu)(bu)(bu)同(tong)(tong)種(zhong)屬(shu)(shu)(shu)或(huo)(huo)(huo)(huo)(huo)類(lei)(lei)型(xing)(xing)(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方(fang)(fang)式(shi)不(bu)(bu)(bu)(bu)同(tong)(tong)。因(yin)(yin)此(ci)(ci),具體(ti)(ti)(ti)(ti)(ti)(ti)(ti)情(qing)況(kuang)請參見(jian)(jian)說(shuo)(shuo)(shuo)(shuo)明(ming)(ming)書(shu)或(huo)(huo)(huo)(huo)(huo)與(yu)技(ji)(ji)術(shu)支持(chi)聯系(xi)(xi)確(que)(que)(que)認。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)般(ban)(ban)要(yao)(yao)(yao)(yao)(yao)(yao)求(qiu):1)于(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)＊-好無溶(rong)血(xue)(xue)(xue)、脂(zhi)(zhi)血(xue)(xue)(xue)、黃-疸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現象(xiang)。組(zu)織樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)將(jiang)(jiang)血(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)洗凈擦干(gan)(gan)后(hou)再進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)稱重(zhong)(zhong)(zhong)(zhong)(zhong)處理(li)(li)(li)(li)(li)(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)處理(li)(li)(li)(li)(li)(li)(li)(li)后(hou)＊-好盡(jin)快(kuai)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且(qie)(qie)保(bao)(bao)存(cun)(cun)(cun)于(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)盒(he)(he)(he)(he)(he)(he)(he)(he)之(zhi)(zhi)(zhi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang),待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing),若不(bu)(bu)(bu)(bu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing),需(xu)(xu)(xu)(xu)離(li)(li)(li)心(xin)(xin)(xin)(xin)后(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特殊指(zhi)(zhi)(zhi)標(biao)除(chu)外(wai)(wai)(wai)(wai))。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)值應(ying)(ying)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)線性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)(fan)圍(wei)內,若超(chao)(chao)過(guo)(guo)(guo)線性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)(fan)圍(wei),需(xu)(xu)(xu)(xu)稀(xi)釋后(hou)再進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若低(di)(di)于(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)(fan)(fan)圍(wei),需(xu)(xu)(xu)(xu)增(zeng)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)濃度(du)(du)后(hou)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。微(wei)(wei)(wei)(wei)(wei)(wei) 量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang) 分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen) 析(xi)(xi)(xi)(xi)(xi)(xi)(xi) 法(fa)(fa)(fa)(fa)(fa)(fa)? ?????????微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)(Microanalysis)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)化(hua)(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，其(qi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)重(zhong)(zhong)(zhong)(zhong)(zhong)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)為(wei)(wei)(wei)(wei)(wei)1-10mg的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)化(hua)(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)方(fang)(fang)法(fa)(fa)(fa)(fa)(fa)(fa)稱為(wei)(wei)(wei)(wei)(wei)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)。有(you)(you)(you)(you)(you)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)之(zhi)(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)，定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)常(chang)(chang)(chang)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)點滴(di)反(fan)(fan)(fan)(fan)應(ying)(ying)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)顯微(wei)(wei)(wei)(wei)(wei)(wei)結(jie)(jie)晶反(fan)(fan)(fan)(fan)應(ying)(ying)等(deng)靈敏(min)度(du)(du)較高(gao)(gao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)方(fang)(fang)法(fa)(fa)(fa)(fa)(fa)(fa)，定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)重(zhong)(zhong)(zhong)(zhong)(zhong)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)、容量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)及(ji)(ji)(ji)儀(yi)器(qi)等(deng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)方(fang)(fang)法(fa)(fa)(fa)(fa)(fa)(fa)。在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)以(yi)(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)重(zhong)(zhong)(zhong)(zhong)(zhong)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)同(tong)(tong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)類(lei)(lei)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)還(huan)(huan)有(you)(you)(you)(you)(you)半微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)超(chao)(chao)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)等(deng)。微(wei)(wei)(wei)(wei)(wei)(wei) 量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang) 和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he) 超(chao)(chao) 微(wei)(wei)(wei)(wei)(wei)(wei) 量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang) 分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen) 析(xi)(xi)(xi)(xi)(xi)(xi)(xi)????????微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)、超(chao)(chao)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)常(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)主(zhu)要(yao)(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)差(cha)別(bie)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)不(bu)(bu)(bu)(bu)同(tong)(tong)。一(yi)(yi)般(ban)(ban)稱樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)0.01克(ke)~0.001克(ke)者(zhe)(zhe)為(wei)(wei)(wei)(wei)(wei)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)，小(xiao)于(yu)(yu)(yu)(yu)0.001克(ke)者(zhe)(zhe)為(wei)(wei)(wei)(wei)(wei)超(chao)(chao)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)。正因(yin)(yin)為(wei)(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)很(hen)少(shao)，所(suo)(suo)以(yi)(yi)(yi)(yi)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)操作(zuo)(zuo)(zuo)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)也和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)常(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)很(hen)不(bu)(bu)(bu)(bu)相同(tong)(tong)。例(li)(li)如(ru)(ru)(ru)(ru)沉淀物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)溶(rong)解(jie)(jie)(jie)度(du)(du)、指(zhi)(zhi)(zhi)示劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)誤差(cha)等(deng)對(dui)(dui)(dui)常(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)影響可(ke)(ke)以(yi)(yi)(yi)(yi)忽(hu)略，而(er)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)超(chao)(chao)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)時(shi)(shi)(shi)(shi)就會引起很(hen)大(da)(da)(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)誤差(cha)。尤(you)其(qi)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)超(chao)(chao)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)要(yao)(yao)(yao)(yao)(yao)(yao)求(qiu)更(geng)(geng)嚴格(ge)(ge)(ge)，須(xu)考(kao)慮(lv)到溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)身在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)空氣中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)蒸(zheng)(zheng)發(fa)(fa)(fa)，因(yin)(yin)此(ci)(ci)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)滴(di)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)時(shi)(shi)(shi)(shi)必(bi)緩在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)潮濕的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)空氣中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)。????????一(yi)(yi)般(ban)(ban)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)器(qi)皿(min)(min)(min)(min)(min)(min)都(dou)很(hen)小(xiao)，按(an)其(qi)要(yao)(yao)(yao)(yao)(yao)(yao)求(qiu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)不(bu)(bu)(bu)(bu)同(tong)(tong)有(you)(you)(you)(you)(you)各(ge)種(zhong)不(bu)(bu)(bu)(bu)同(tong)(tong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)形(xing)狀(zhuang)(zhuang)。各(ge)種(zhong)儀(yi)器(qi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)法(fa)(fa)(fa)(fa)(fa)(fa)經過(guo)(guo)(guo)適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)改進(jin)(jin)(jin)(jin)(jin)后(hou)，可(ke)(ke)以(yi)(yi)(yi)(yi)直接應(ying)(ying)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)。超(chao)(chao)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)由(you)于(yu)(yu)(yu)(yu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)太少(shao)，因(yin)(yin)此(ci)(ci)必(bi)須(xu)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)顯微(wei)(wei)(wei)(wei)(wei)(wei)鏡下(xia)(xia)(xia)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)所(suo)(suo)有(you)(you)(you)(you)(you)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)操作(zuo)(zuo)(zuo)(如(ru)(ru)(ru)(ru)沉淀和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)濡(ru)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)等(deng))，某(mou)些(xie)(xie)儀(yi)器(qi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)(如(ru)(ru)(ru)(ru)電(dian)位滴(di)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)，電(dian)解(jie)(jie)(jie)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)電(dian)導等(deng))，也可(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)來(lai)作(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)(wei)超(chao)(chao)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)之(zhi)(zhi)(zhi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。????????微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)研究(jiu)(jiu)工作(zuo)(zuo)(zuo)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)得(de)(de)極廣。尤(you)其(qi)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)稀(xi)有(you)(you)(you)(you)(you)元(yuan)素(su)化(hua)(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)研究(jiu)(jiu)時(shi)(shi)(shi)(shi)，在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)解(jie)(jie)(jie)決地球化(hua)(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)同(tong)(tong)題，研究(jiu)(jiu)某(mou)些(xie)(xie)稀(xi)有(you)(you)(you)(you)(you)元(yuan)素(su)礦物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)粗(cu)成(cheng)(cheng)(cheng)(cheng)及(ji)(ji)(ji)其(qi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)配(pei)(pei)規(gui)律時(shi)(shi)(shi)(shi)，往(wang)往(wang)只(zhi)(zhi)能(neng)(neng)得(de)(de)到極少(shao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)，在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)這(zhe)選(xuan)種(zhong)情(qing)況(kuang)下(xia)(xia)(xia)一(yi)(yi)般(ban)(ban)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)常(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)法(fa)(fa)(fa)(fa)(fa)(fa)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)無法(fa)(fa)(fa)(fa)(fa)(fa)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，只(zhi)(zhi)有(you)(you)(you)(you)(you)靠(kao)(kao)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)法(fa)(fa)(fa)(fa)(fa)(fa)來(lai)解(jie)(jie)(jie)決。另外(wai)(wai)(wai)(wai)，在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)化(hua)(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)(ying)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)更(geng)(geng)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)普遍3-磷酸(suan)(suan)甘(gan)(gan)油酸(suan)(suan)激酶(mei)(mei)(mei)(mei)(PGK)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)科(ke)研實(shi)驗,不(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)臨床。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)意(yi)(yi)(yi)(yi)義:輔酶(mei)(mei)(mei)(mei)NAD(H)廣泛存(cun)(cun)(cun)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)于(yu)(yu)(yu)(yu)動(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、植物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、微(wei)(wei)(wei)(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)培養細(xi)(xi)(xi)胞(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),NAD+是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)糖(tang)酵(jiao)解(jie)(jie)(jie)(EMP)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)三(san)(san)羧酸(suan)(suan)循環(TCA)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要(yao)(yao)(yao)(yao)(yao)(yao)氫(qing)受(shou)體(ti)(ti)(ti)(ti)(ti)(ti)(ti),生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?NADH?經呼吸(xi)電(dian)子(zi)鏈(ETC)傳遞把(ba)電(dian)子(zi)交給氧,在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)合(he)成(cheng)(cheng)(cheng)(cheng)?ATP?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)(tong)時(shi)(shi)(shi)(shi),形(xing)成(cheng)(cheng)(cheng)(cheng)大(da)(da)(da)(da)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?ROS,同(tong)(tong)時(shi)(shi)(shi)(shi)?NADH?再生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)為(wei)(wei)(wei)(wei)(wei)?NAD+。糖(tang)、脂(zhi)(zhi)、蛋白質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)三(san)(san)大(da)(da)(da)(da)代(dai)謝物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)解(jie)(jie)(jie)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)氧化(hua)(hua)(hua)(hua)(hua)(hua)反(fan)(fan)(fan)(fan)應(ying)(ying)絕大(da)(da)(da)(da)部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)通過(guo)(guo)(guo)這(zhe)一(yi)(yi)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)完(wan)(wan)成(cheng)(cheng)(cheng)(cheng)。NAD(H)含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH/NAD+比(bi)(bi)(bi)值的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)高(gao)(gao)低(di)(di)可(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)評價(jia)糖(tang)酵(jiao)解(jie)(jie)(jie)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?TCA?循環的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)強弱。較高(gao)(gao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)NAD(H)及(ji)(ji)(ji)?NADH/NAD+比(bi)(bi)(bi)值說(shuo)(shuo)(shuo)(shuo)明(ming)(ming)細(xi)(xi)(xi)胞(bao)(bao)呼吸(xi)耗氧量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)較高(gao)(gao),處于(yu)(yu)(yu)(yu)過(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)(hua)(hua)狀(zhuang)(zhuang)態(tai)。此(ci)(ci)外(wai)(wai)(wai)(wai),NADH/NAD+比(bi)(bi)(bi)值升高(gao)(gao)也可(ke)(ke)抑制(zhi)糖(tang)酵(jiao)解(jie)(jie)(jie)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?TCA?循環。另外(wai)(wai)(wai)(wai),NAD+降解(jie)(jie)(jie)產(chan)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)對(dui)(dui)(dui)細(xi)(xi)(xi)胞(bao)(bao)信號傳導、代(dai)謝和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)基(ji)因(yin)(yin)表達等(deng)具有(you)(you)(you)(you)(you)重(zhong)(zhong)(zhong)(zhong)(zhong)要(yao)(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)調控作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)原(yuan)(yuan)理(li)(li)(li)(li)(li)(li)(li)(li):分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH,NADH?通過(guo)(guo)(guo)?PMS?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)遞氫(qing)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong),還(huan)(huan)原(yuan)(yuan)氧化(hua)(hua)(hua)(hua)(hua)(hua)型(xing)(xing)(xing)噻唑藍(lan)(MTT)為(wei)(wei)(wei)(wei)(wei)甲瓚(zan),在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)?570nm?下(xia)(xia)(xia)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)吸(xi)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)值;而(er)?NAD+可(ke)(ke)被(bei)乙醇(chun)脫氫(qing)酶(mei)(mei)(mei)(mei)還(huan)(huan)原(yuan)(yuan)為(wei)(wei)(wei)(wei)(wei)?NADH,進(jin)(jin)(jin)(jin)(jin)一(yi)(yi)步(bu)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)?MTT?還(huan)(huan)原(yuan)(yuan)法(fa)(fa)(fa)(fa)(fa)(fa)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。需(xu)(xu)(xu)(xu)自備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)儀(yi)器(qi)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)品(pin)(pin)(pin):酶(mei)(mei)(mei)(mei)標(biao)儀(yi)、臺式(shi)離(li)(li)(li)心(xin)(xin)(xin)(xin)機(ji)(ji)、移液(ye)(ye)(ye)(ye)(ye)(ye)器(qi)、96?孔板(ban)、研缽、冰(bing)(bing)(bing)(bing)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)蒸(zheng)(zheng)餾(liu)水(shui)(shui)(shui)(shui)(shui)。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)成(cheng)(cheng)(cheng)(cheng)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)配(pei)(pei)制(zhi):酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping),4℃保(bao)(bao)存(cun)(cun)(cun);堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping),4℃保(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi):液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?10 mL×1?瓶(ping)(ping),4℃保(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)二(er):液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?3 mL×1?瓶(ping)(ping),4℃保(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)三(san)(san):粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping)(ping),-20?℃保(bao)(bao)存(cun)(cun)(cun),用(yong)(yong)(yong)(yong)(yong)(yong)(yong)時(shi)(shi)(shi)(shi)加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?3mL?蒸(zheng)(zheng)餾(liu)水(shui)(shui)(shui)(shui)(shui),混(hun)勻(yun)(yun),用(yong)(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)完(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)存(cun)(cun)(cun)一(yi)(yi)周;試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)四(si):粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping)(ping),4?℃保(bao)(bao)存(cun)(cun)(cun),用(yong)(yong)(yong)(yong)(yong)(yong)(yong)時(shi)(shi)(shi)(shi)加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?3mL?蒸(zheng)(zheng)餾(liu)水(shui)(shui)(shui)(shui)(shui),混(hun)勻(yun)(yun),用(yong)(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)完(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)存(cun)(cun)(cun)一(yi)(yi)周;試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)五:液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?3.6mL×1?瓶(ping)(ping),4?℃保(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu):液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?30mL×1?瓶(ping)(ping),4?℃保(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)七:液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping),4?℃保(bao)(bao)存(cun)(cun)(cun)。NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):1?血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)照(zhao)(zhao)(zhao)(zhao)(zhao)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL):酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(li)(建(jian)(jian)(jian)(jian)議(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)?0.1mL?血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)),加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?1mL?酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)?5min(蓋(gai)(gai)緊,以(yi)(yi)(yi)(yi)防(fang)(fang)止(zhi)(zhi)(zhi)(zhi)水(shui)(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散失(shi)(shi));冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻后(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?500μL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)照(zhao)(zhao)(zhao)(zhao)(zhao)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL):堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(li)(建(jian)(jian)(jian)(jian)議(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)?0.1mL血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)),加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?1mL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)?5min(蓋(gai)(gai)緊,以(yi)(yi)(yi)(yi)防(fang)(fang)止(zhi)(zhi)(zhi)(zhi)水(shui)(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散失(shi)(shi));冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻后(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?500μL?酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。2?組(zu)織中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)照(zhao)(zhao)(zhao)(zhao)(zhao)組(zu)織質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(g):酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(li)(建(jian)(jian)(jian)(jian)議(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)?0.1g?組(zu)織,加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?1mL酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)研磨(mo),95℃水(shui)(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)?5min(蓋(gai)(gai)緊,以(yi)(yi)(yi)(yi)防(fang)(fang)止(zhi)(zhi)(zhi)(zhi)水(shui)(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散失(shi)(shi));冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻后(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?500μL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)照(zhao)(zhao)(zhao)(zhao)(zhao)組(zu)織質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(g):堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(li)(建(jian)(jian)(jian)(jian)議(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)?0.1g?組(zu)織,加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?1mL堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)研磨(mo),95℃水(shui)(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)?5min(蓋(gai)(gai)緊,以(yi)(yi)(yi)(yi)防(fang)(fang)止(zhi)(zhi)(zhi)(zhi)水(shui)(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散失(shi)(shi));冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻后(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?500μL?酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3?細(xi)(xi)(xi)胞(bao)(bao)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)菌(jun)(jun)(jun)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先收(shou)集細(xi)(xi)(xi)胞(bao)(bao)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)菌(jun)(jun)(jun)到離(li)(li)(li)心(xin)(xin)(xin)(xin)管(guan)(guan)(guan)(guan)(guan)(guan)內,棄上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),按(an)照(zhao)(zhao)(zhao)(zhao)(zhao)細(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)胞(bao)(bao)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)(ge)):酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(li)(建(jian)(jian)(jian)(jian)議(yi)?500?萬細(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)胞(bao)(bao)加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?1mL?酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),超(chao)(chao)聲(sheng)波(bo)破碎(sui)(sui)(冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu),功(gong)率20%或(huo)(huo)(huo)(huo)(huo)?200W,超(chao)(chao)聲(sheng)?3s,間隔?10s,重(zhong)(zhong)(zhong)(zhong)(zhong)復(fu)(fu)?30?次(ci)(ci)(ci)(ci)),95℃水(shui)(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)?5min(蓋(gai)(gai)緊,以(yi)(yi)(yi)(yi)防(fang)(fang)止(zhi)(zhi)(zhi)(zhi)水(shui)(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散失(shi)(shi));冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻后(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?500μL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先收(shou)集細(xi)(xi)(xi)胞(bao)(bao)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)菌(jun)(jun)(jun)到離(li)(li)(li)心(xin)(xin)(xin)(xin)管(guan)(guan)(guan)(guan)(guan)(guan)內,棄上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),按(an)照(zhao)(zhao)(zhao)(zhao)(zhao)細(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)胞(bao)(bao)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)(ge)):堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(li)(建(jian)(jian)(jian)(jian)議(yi)?500?萬細(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)胞(bao)(bao)加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?1mL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),超(chao)(chao)聲(sheng)波(bo)破碎(sui)(sui)(冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu),功(gong)率20%或(huo)(huo)(huo)(huo)(huo)?200W,超(chao)(chao)聲(sheng)?3s,間隔?10s,重(zhong)(zhong)(zhong)(zhong)(zhong)復(fu)(fu)?30?次(ci)(ci)(ci)(ci)),95℃水(shui)(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)?5min(蓋(gai)(gai)緊,以(yi)(yi)(yi)(yi)防(fang)(fang)止(zhi)(zhi)(zhi)(zhi)水(shui)(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散失(shi)(shi));冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻后(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?500μL?酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。充(chong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)混(hun)勻(yun)(yun),靜置(zhi)(zhi)?5min?后(hou),20000g,25℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?5min,棄上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),沉淀中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)入(ru)(ru)(ru):混(hun)勻(yun)(yun),取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?200μL?轉(zhuan)移至微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)石(shi)英比(bi)(bi)(bi)色(se)皿(min)(min)(min)(min)(min)(min)或(huo)(huo)(huo)(huo)(huo)?96?孔板(ban)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),570nm?下(xia)(xia)(xia)讀取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)對(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)吸(xi)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)值?A1?和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)吸(xi)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)值?A2,計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)△A=A2-A1。注(zhu)意(yi)(yi)(yi)(yi)事項:1、如(ru)(ru)(ru)(ru)果(guo)一(yi)(yi)次(ci)(ci)(ci)(ci)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)數(shu)較多,可(ke)(ke)將(jiang)(jiang)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)、二(er)、三(san)(san)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)四(si)按(an)比(bi)(bi)(bi)例(li)(li)配(pei)(pei)成(cheng)(cheng)(cheng)(cheng)混(hun)合(he)液(ye)(ye)(ye)(ye)(ye)(ye)。2、對(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)步(bu)驟的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie):對(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)加(jia)(jia)(jia)(jia)完(wan)(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)、二(er)、三(san)(san)、四(si)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)五后(hou)必(bi)須(xu)馬上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)加(jia)(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu);測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)加(jia)(jia)(jia)(jia)完(wan)(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)、二(er)、三(san)(san)、四(si)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)五后(hou)必(bi)須(xu)反(fan)(fan)(fan)(fan)應(ying)(ying)?20min?后(hou)再加(jia)(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu)。3、反(fan)(fan)(fan)(fan)應(ying)(ying)過(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)注(zhu)意(yi)(yi)(yi)(yi)避光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)。4、若?NAD+測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0302,NADH?測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0222,說(shuo)(shuo)(shuo)(shuo)明(ming)(ming)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)輔酶(mei)(mei)(mei)(mei)含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)較低(di)(di),已低(di)(di)于(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian),可(ke)(ke)做(zuo)如(ru)(ru)(ru)(ru)下(xia)(xia)(xia)調整(zheng):(1)將(jiang)(jiang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)避光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)靜置(zhi)(zhi)時(shi)(shi)(shi)(shi)間?20min?延長(chang)到?60min;(2)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)階(jie)段增(zeng)加(jia)(jia)(jia)(jia)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang),即(ji)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?0.2g?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)或(huo)(huo)(huo)(huo)(huo)?0.2mL?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?1mL?提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)。5、由(you)于(yu)(yu)(yu)(yu)每(mei)一(yi)(yi)個(ge)(ge)(ge)(ge)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)設一(yi)(yi)個(ge)(ge)(ge)(ge)對(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan),本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)?100?管(guan)(guan)(guan)(guan)(guan)(guan)保(bao)(bao)證(zheng)(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)?48?個(ge)(ge)(ge)(ge)?NAD+或(huo)(huo)(huo)(huo)(huo)?NADH.NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan):(一(yi)(yi)) NAD+含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)標(biao)準條件(jian)下(xia)(xia)(xia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回(hui)歸(gui)曲線為(wei)(wei)(wei)(wei)(wei)?y = 0.1475x + 0.0302,R2 = 0.9978;其(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)(wei)(wei)?NAD+濃度(du)(du)?nmol/mL1、血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)NAD+含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)=[(△A -0.0302)÷0.1475×V1) ]÷(V3×V1÷V2)=135.6×(△A -0.0302)2、組(zu)織、細(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)胞(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(1)按(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋白濃度(du)(du)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)NAD+(nmol/mg prot)=[(△A -0.0302)÷0.1475×V1)]÷(V1×Cpr)= 6.8×(△A -0.0302)÷Cpr(2)按(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)鮮(xian)(xian)重(zhong)(zhong)(zhong)(zhong)(zhong)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)NAD+(nmol/g?鮮(xian)(xian)重(zhong)(zhong)(zhong)(zhong)(zhong))= [(△A -0.0302)÷0.1475×V1)]÷(W×V1÷V2)= 13.6×(△A -0.0302)÷W(3)按(an)細(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)胞(bao)(bao)密度(du)(du)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)NAD+(nmol/104 cell)=[(△A -0.0302)÷0.1475×V1)]÷(500×V1÷V2)=0.027×(△A -0.0302)(二(er))NADH?含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)標(biao)準條件(jian)下(xia)(xia)(xia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回(hui)歸(gui)曲線為(wei)(wei)(wei)(wei)(wei)?y = 0.1404x + 0.0222,R2 = 0.9976;其(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)(wei)(wei)?NADH?濃度(du)(du)?nmol/mL1、血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)NADH?含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)= [ (△A -0.0222)÷0.1404×V1) ]÷(V3×V1÷V2)= 142.5×(△A -0.0222)2、組(zu)織、細(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)胞(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(1)按(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋白濃度(du)(du)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)NADH (nmol/mg prot)= [ (△A -0.0222)÷0.1404×V1) ]÷(V1×Cpr)= 7.1×(△A -0.0222)÷Cpr(2)按(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)鮮(xian)(xian)重(zhong)(zhong)(zhong)(zhong)(zhong)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)NADH (nmol/g?鮮(xian)(xian)重(zhong)(zhong)(zhong)(zhong)(zhong))=[(△A -0.0222)÷0.1404×V1) ]÷(W×V1÷V2)= 14.2×(△A -0.0222)÷W(3)按(an)細(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)胞(bao)(bao)密度(du)(du)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)NADH (nmol/104 cell)= [ (△A -0.0222)÷0.1404×V1) ]÷(500×V1÷V2)=0.028×(△A -0.0222)V1:加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)反(fan)(fan)(fan)(fan)應(ying)(ying)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji),0.02mL;V2:加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji),2mL;V3:加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji):0.1mL;Cpr:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋白質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)濃度(du)(du),mg/mL; W:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang),g;500:細(xi)(xi)(xi)胞(bao)(bao)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)菌(jun)(jun)(jun)總數(shu),500?萬。注(zhu)?意(yi)(yi)(yi)(yi):?檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian)為(wei)(wei)(wei)(wei)(wei)?0.1nmol/mL?或(huo)(huo)(huo)(huo)(huo)?0.1nmol/g?鮮(xian)(xian)重(zhong)(zhong)(zhong)(zhong)(zhong)?或(huo)(huo)(huo)(huo)(huo)?0.001nmol/mg prot

果(guo)(guo)(guo)(guo)(guo)糖(tang)-1,6-二磷酸(suan)(suan)醛縮酶(mei)(mei)(FBA)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)科(ke)研實(shi)驗(yan),不(bu)(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)臨(lin)床。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)可(ke)(ke)以(yi)(yi)(yi)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)型(xing)(xing)(xing)有(you)(you)(you)(you)哪些???生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗(yan)原(yuan)(yuan)理(li)(li)(li)(li)(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)基(ji)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)反(fan)應(ying),理(li)(li)(li)(li)(li)(li)論(lun)上(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)(bu)受(shou)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)種(zhong)屬及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)型(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)制(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通常(chang)(chang)(chang)只(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)型(xing)(xing)(xing),不(bu)(bu)(bu)(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)種(zhong)屬。但是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)某(mou)(mou)些指(zhi)(zhi)標(biao)(biao)在(zai)(zai)(zai)不(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)種(zhong)屬或(huo)(huo)(huo)(huo)(huo)類(lei)(lei)型(xing)(xing)(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方(fang)式(shi)(shi)不(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)。因(yin)(yin)(yin)此(ci)(ci)(ci),具體(ti)(ti)(ti)(ti)(ti)情(qing)況請參見說明(ming)書(shu)或(huo)(huo)(huo)(huo)(huo)與技(ji)(ji)術(shu)支持聯(lian)(lian)系(xi)確(que)(que)認。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)般(ban)(ban)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)求:1)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)言(yan)(yan),樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)無溶血(xue)、脂血(xue)、的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現象(xiang)。組(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)(xu)將(jiang)(jiang)血(xue)液(ye)(ye)(ye)(ye)(ye)洗(xi)凈(jing)(jing)擦(ca)干(gan)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)(zai)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)稱(cheng)重(zhong)(zhong)(zhong)(zhong)處(chu)(chu)(chu)(chu)理(li)(li)(li)(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)處(chu)(chu)(chu)(chu)理(li)(li)(li)(li)(li)(li)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)盡(jin)快進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且保(bao)(bao)存(cun)(cun)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)盒(he)(he)(he)(he)(he)(he)(he)(he)之(zhi)上(shang)(shang)(shang),待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。求3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)(xu)澄(cheng)(cheng)(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),若(ruo)(ruo)不(bu)(bu)(bu)(bu)(bu)(bu)澄(cheng)(cheng)(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),需(xu)(xu)(xu)(xu)(xu)離(li)(li)(li)心(xin)(xin)(xin)(xin)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特(te)(te)殊(shu)指(zhi)(zhi)標(biao)(biao)除外(wai))。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)值(zhi)應(ying)在(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)線(xian)性(xing)(xing)(xing)(xing)(xing)(xing)范圍(wei)(wei)內,若(ruo)(ruo)超(chao)(chao)(chao)過(guo)(guo)(guo)(guo)(guo)線(xian)性(xing)(xing)(xing)(xing)(xing)(xing)范圍(wei)(wei),需(xu)(xu)(xu)(xu)(xu)稀釋后(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)(zai)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若(ruo)(ruo)低于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范圍(wei)(wei),需(xu)(xu)(xu)(xu)(xu)增加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)濃(nong)度(du)(du)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)(fa)和(he)(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)(guang)度(du)(du)法(fa)(fa)(fa)(fa)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別?分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)(guang)度(du)(du)法(fa)(fa)(fa)(fa):主流測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)系(xi)為(wei)(wei)1mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)器(qi)(qi)(qi)(qi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)(guang)度(du)(du)計(ji)(ji)(ji)(ji)(ji),能(neng)(neng)(neng)夠(gou)大(da)限(xian)(xian)度(du)(du)地(di)滿足絕大(da)多數(shu)用(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)。微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)(fa):主流測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)系(xi)為(wei)(wei)0.2mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)器(qi)(qi)(qi)(qi)或(huo)(huo)(huo)(huo)(huo)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)(guang)度(du)(du)計(ji)(ji)(ji)(ji)(ji),測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)更(geng)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)簡便快捷。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)規(gui)格(ge)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)100管(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)(he)50管(guan)(guan)(guan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)什(shen)么意(yi)(yi)(yi)思?(1)50管(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):50管(guan)(guan)(guan)指(zhi)(zhi)能(neng)(neng)(neng)夠(gou)做50次(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)(zhi)可(ke)(ke)以(yi)(yi)(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)48個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)。如(ru)(ru)(ru)(ru)(ru)果(guo)(guo)(guo)(guo)(guo)每個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)重(zhong)(zhong)(zhong)(zhong)復(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci)(ci),那(nei)么只(zhi)(zhi)能(neng)(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)16個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)。其(qi)(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)(you)2次(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)空(kong)白(bai)(bai)管(guan)(guan)(guan)或(huo)(huo)(huo)(huo)(huo)者(zhe)(zhe)對(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)。值(zhi)得(de)特(te)(te)別注(zhu)(zhu)意(yi)(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi),50管(guan)(guan)(guan)/24樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)每個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)(dou)需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)做對(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao),因(yin)(yin)(yin)此(ci)(ci)(ci)能(neng)(neng)(neng)夠(gou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)僅為(wei)(wei)8個(ge)(ge)(ge)(假設做3次(ci)(ci)(ci)重(zhong)(zhong)(zhong)(zhong)復(fu))(2)100管(guan)(guan)(guan)/96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):100管(guan)(guan)(guan)指(zhi)(zhi)能(neng)(neng)(neng)夠(gou)做100次(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)(zhi)可(ke)(ke)以(yi)(yi)(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)96個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin),如(ru)(ru)(ru)(ru)(ru)果(guo)(guo)(guo)(guo)(guo)每個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)重(zhong)(zhong)(zhong)(zhong)復(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci)(ci),那(nei)么只(zhi)(zhi)能(neng)(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)32個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)。其(qi)(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)(you)4次(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)空(kong)白(bai)(bai)管(guan)(guan)(guan)或(huo)(huo)(huo)(huo)(huo)者(zhe)(zhe)對(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)。注(zhu)(zhu)意(yi)(yi)(yi)由(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)瓶(ping)璧和(he)(he)(he)(he)(he)(he)(he)(he)移液(ye)(ye)(ye)(ye)(ye)器(qi)(qi)(qi)(qi)吸附(fu)等(deng),通常(chang)(chang)(chang)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)比(bi)(bi)(bi)理(li)(li)(li)(li)(li)(li)論(lun)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)次(ci)(ci)(ci)數(shu)少(shao)1~3次(ci)(ci)(ci)。其(qi)(qi)(qi)(qi)(qi)它(ta)規(gui)格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)義依(yi)次(ci)(ci)(ci)類(lei)(lei)推。值(zhi)得(de)特(te)(te)別注(zhu)(zhu)意(yi)(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi),100管(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)每個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)(dou)需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)做對(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao),因(yin)(yin)(yin)此(ci)(ci)(ci)能(neng)(neng)(neng)夠(gou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)僅為(wei)(wei)16個(ge)(ge)(ge)(假設做3次(ci)(ci)(ci)重(zhong)(zhong)(zhong)(zhong)復(fu))。如(ru)(ru)(ru)(ru)(ru)何(he)(he)防(fang)(fang)止(zhi)(zhi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)失(shi)(shi)(shi)效?????不(bu)(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)過(guo)(guo)(guo)(guo)(guo)早訂購,以(yi)(yi)(yi)免(mian)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)失(shi)(shi)(shi)效.收到(dao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)后(hou)(hou)(hou)(hou)(hou)(hou)(hou),檢(jian)(jian)(jian)(jian)(jian)查外(wai)包裝是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)正(zheng)(zheng)(zheng)常(chang)(chang)(chang)。馬上(shang)(shang)(shang)打(da)開,按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)說明(ming)書(shu)核對(dui)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)正(zheng)(zheng)(zheng)確(que)(que),注(zhu)(zhu)意(yi)(yi)(yi)瓶(ping)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)狀態是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)與說明(ming)書(shu)一(yi)(yi)(yi)(yi)(yi)致。如(ru)(ru)(ru)(ru)(ru)果(guo)(guo)(guo)(guo)(guo)有(you)(you)(you)(you)問題,或(huo)(huo)(huo)(huo)(huo)者(zhe)(zhe)懷疑,馬上(shang)(shang)(shang)聯(lian)(lian)系(xi)公(gong)司銷售,進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)確(que)(que)認。如(ru)(ru)(ru)(ru)(ru)果(guo)(guo)(guo)(guo)(guo)沒有(you)(you)(you)(you)問題,請務必按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)說明(ming)書(shu)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)存(cun)(cun)。注(zhu)(zhu)意(yi)(yi)(yi),不(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji),可(ke)(ke)能(neng)(neng)(neng)需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別保(bao)(bao)存(cun)(cun)在(zai)(zai)(zai)常(chang)(chang)(chang)溫(25°℃)、4℃和(he)(he)(he)(he)(he)(he)(he)(he)-20℃,務必分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)求保(bao)(bao)存(cun)(cun),不(bu)(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)整(zheng)個(ge)(ge)(ge)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)放置(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)箱(xiang)。如(ru)(ru)(ru)(ru)(ru)何(he)(he)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)預測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)?(1)務必在(zai)(zai)(zai)7個(ge)(ge)(ge)工作(zuo)(zuo)日(ri)內進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)預測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),以(yi)(yi)(yi)確(que)(que)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)該試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)適合(he)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin),以(yi)(yi)(yi)免(mian)造成(cheng)(cheng)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)和(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浪(lang)費(fei)(fei)。(2)選(xuan)(xuan)擇2~3個(ge)(ge)(ge)預期差(cha)(cha)異(yi)比(bi)(bi)(bi)較(jiao)大(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin),嚴(yan)格(ge)按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)說明(ming)書(shu)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)操作(zuo)(zuo),注(zhu)(zhu)意(yi)(yi)(yi)儀(yi)器(qi)(qi)(qi)(qi)工作(zuo)(zuo)狀態,操作(zuo)(zuo)規(gui)范,控(kong)制(zhi)(zhi)(zhi)好(hao)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)條件(jian),尤(you)其(qi)(qi)(qi)(qi)(qi)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)溫度(du)(du)。(3)如(ru)(ru)(ru)(ru)(ru)實(shi)與公(gong)司銷售溝通預測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結果(guo)(guo)(guo)(guo)(guo),公(gong)司技(ji)(ji)術(shu)人(ren)員會(hui)確(que)(que)認測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結果(guo)(guo)(guo)(guo)(guo)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)正(zheng)(zheng)(zheng)常(chang)(chang)(chang),是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)調整(zheng),如(ru)(ru)(ru)(ru)(ru)何(he)(he)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)調整(zheng),從(cong)而(er)確(que)(que)保(bao)(bao)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結果(guo)(guo)(guo)(guo)(guo)可(ke)(ke)靠(kao)。熒(ying)(ying)(ying)(ying) 光(guang)(guang)(guang)(guang)(guang)(guang) 分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen) 析(xi)(xi)(xi)(xi)(xi) 法(fa)(fa)(fa)(fa)?????????熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)法(fa)(fa)(fa)(fa)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)指(zhi)(zhi)利用(yong)(yong)(yong)(yong)(yong)(yong)某(mou)(mou)些物(wu)(wu)(wu)(wu)(wu)質(zhi)被(bei)紫外(wai)光(guang)(guang)(guang)(guang)(guang)(guang)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)射(she)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)處(chu)(chu)(chu)(chu)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)激發(fa)態，激發(fa)態分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)經(jing)(jing)歷(li)一(yi)(yi)(yi)(yi)(yi)個(ge)(ge)(ge)碰撞及(ji)發(fa)射(she)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)去激發(fa)過(guo)(guo)(guo)(guo)(guo)程(cheng)所發(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)能(neng)(neng)(neng)反(fan)映出該物(wu)(wu)(wu)(wu)(wu)質(zhi)特(te)(te)性(xing)(xing)(xing)(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)，可(ke)(ke)以(yi)(yi)(yi)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)或(huo)(huo)(huo)(huo)(huo)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)法(fa)(fa)(fa)(fa)。由(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)有(you)(you)(you)(you)些物(wu)(wu)(wu)(wu)(wu)質(zhi)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)身不(bu)(bu)(bu)(bu)(bu)(bu)發(fa)射(she)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)（或(huo)(huo)(huo)(huo)(huo)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)很(hen)(hen)弱），這(zhe)就需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)把不(bu)(bu)(bu)(bu)(bu)(bu)發(fa)射(she)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)質(zhi)轉(zhuan)化(hua)(hua)(hua)(hua)(hua)成(cheng)(cheng)能(neng)(neng)(neng)發(fa)射(she)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)質(zhi)。例(li)(li)如(ru)(ru)(ru)(ru)(ru)用(yong)(yong)(yong)(yong)(yong)(yong)某(mou)(mou)些試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)（如(ru)(ru)(ru)(ru)(ru)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)染(ran)料），使其(qi)(qi)(qi)(qi)(qi)與不(bu)(bu)(bu)(bu)(bu)(bu)發(fa)射(she)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)質(zhi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)絡(luo)合(he)物(wu)(wu)(wu)(wu)(wu)，各(ge)種(zhong)絡(luo)合(he)物(wu)(wu)(wu)(wu)(wu)能(neng)(neng)(neng)發(fa)射(she)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)，再(zai)(zai)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)。因(yin)(yin)(yin)此(ci)(ci)(ci)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)使用(yong)(yong)(yong)(yong)(yong)(yong)，對(dui)一(yi)(yi)(yi)(yi)(yi)些原(yuan)(yuan)來(lai)(lai)不(bu)(bu)(bu)(bu)(bu)(bu)發(fa)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)無機(ji)物(wu)(wu)(wu)(wu)(wu)質(zhi)和(he)(he)(he)(he)(he)(he)(he)(he)有(you)(you)(you)(you)機(ji)物(wu)(wu)(wu)(wu)(wu)質(zhi)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)熒(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)打(da)開了(le)(le)大(da)門，擴展了(le)(le)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍(wei)(wei)。比(bi)(bi)(bi)色(se)皿(min)(min)有(you)(you)(you)(you)哪些規(gui)格(ge)?(1)常(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)比(bi)(bi)(bi)色(se)皿(min)(min),如(ru)(ru)(ru)(ru)(ru)1mL和(he)(he)(he)(he)(he)(he)(he)(he)3mL等(deng),常(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)都(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)選(xuan)(xuan)用(yong)(yong)(yong)(yong)(yong)(yong)1mL體(ti)(ti)(ti)(ti)(ti)系(xi),只(zhi)(zhi)能(neng)(neng)(neng)用(yong)(yong)(yong)(yong)(yong)(yong)1mL比(bi)(bi)(bi)色(se)皿(min)(min),不(bu)(bu)(bu)(bu)(bu)(bu)可(ke)(ke)以(yi)(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong)更(geng)大(da)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)色(se)皿(min)(min),否(fou)(fou)則試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)(bu)(bu)夠(gou),無法(fa)(fa)(fa)(fa)正(zheng)(zheng)(zheng)常(chang)(chang)(chang)比(bi)(bi)(bi)色(se)。(2)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)比(bi)(bi)(bi)色(se)皿(min)(min),如(ru)(ru)(ru)(ru)(ru)0.25mL和(he)(he)(he)(he)(he)(he)(he)(he)0.5mL等(deng),微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)都(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)選(xuan)(xuan)用(yong)(yong)(yong)(yong)(yong)(yong)0.25mL比(bi)(bi)(bi)色(se)皿(min)(min)。一(yi)(yi)(yi)(yi)(yi)般(ban)(ban)客(ke)戶(hu)都(dou)(dou)選(xuan)(xuan)擇使用(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)儀(yi)(需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)自備(bei)(bei)酶(mei)(mei)標(biao)(biao)板(ban))(3)紫外(wai)波(bo)長檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)石(shi)英比(bi)(bi)(bi)色(se)皿(min)(min),不(bu)(bu)(bu)(bu)(bu)(bu)可(ke)(ke)以(yi)(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong)普通玻璃比(bi)(bi)(bi)色(se)皿(min)(min)。(4)不(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)規(gui)格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)色(se)皿(min)(min),其(qi)(qi)(qi)(qi)(qi)外(wai)觀大(da)小是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)(yi)(yi)(yi)致的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de),都(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)1cm光(guang)(guang)(guang)(guang)(guang)(guang)徑(也有(you)(you)(you)(you)0.5cm光(guang)(guang)(guang)(guang)(guang)(guang)徑的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)色(se)皿(min)(min),不(bu)(bu)(bu)(bu)(bu)(bu)常(chang)(chang)(chang)見,也可(ke)(ke)以(yi)(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong),但是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)時(shi)(shi)(shi)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)注(zhu)(zhu)意(yi)(yi)(yi)修改計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)公(gong)式(shi)(shi)),其(qi)(qi)(qi)(qi)(qi)內容(rong)積(ji)(ji)(ji)差(cha)(cha)異(yi)在(zai)(zai)(zai)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)壁(bi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)厚度(du)(du)。代(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)優點(dian)(1)更(geng)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)省事兒,完全不(bu)(bu)(bu)(bu)(bu)(bu)必為(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)犯難。用(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)只(zhi)(zhi)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)供樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)和(he)(he)(he)(he)(he)(he)(he)(he)想(xiang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)指(zhi)(zhi)標(biao)(biao),就不(bu)(bu)(bu)(bu)(bu)(bu)再(zai)(zai)需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)為(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)操心(xin)(xin)(xin)(xin)了(le)(le),不(bu)(bu)(bu)(bu)(bu)(bu)再(zai)(zai)受(shou)到(dao)操作(zuo)(zuo)技(ji)(ji)能(neng)(neng)(neng)、儀(yi)器(qi)(qi)(qi)(qi)設備(bei)(bei)和(he)(he)(he)(he)(he)(he)(he)(he)時(shi)(shi)(shi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)制(zhi)(zhi)(zhi),就等(deng)著拿到(dao)數(shu)據寫論(lun)文。(2)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)數(shu)據更(geng)有(you)(you)(you)(you)保(bao)(bao)障(zhang)。嚴(yan)格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)質(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)管(guan)(guan)(guan)理(li)(li)(li)(li)(li)(li)體(ti)(ti)(ti)(ti)(ti)系(xi),從(cong)而(er)保(bao)(bao)證(zheng)了(le)(le)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結果(guo)(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)真(zhen)實(shi)可(ke)(ke)靠(kao)。我們還(huan)建(jian)立了(le)(le)免(mian)費(fei)(fei)預測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)制(zhi)(zhi)(zhi)度(du)(du)。(3)防(fang)(fang)止(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)損失(shi)(shi)(shi)無論(lun)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)自己完成(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)全過(guo)(guo)(guo)(guo)(guo)程(cheng),還(huan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)購買試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)完成(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),都(dou)(dou)可(ke)(ke)能(neng)(neng)(neng)因(yin)(yin)(yin)為(wei)(wei)缺乏測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)經(jing)(jing)驗(yan),導(dao)致測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)失(shi)(shi)(shi)敗(bai),從(cong)而(er)損失(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情(qing)況。對(dui)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)命科(ke)學(xue)(xue)研究(jiu)(jiu)而(er)言(yan)(yan),樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)＊珍(zhen)貴(gui)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)一(yi)(yi)(yi)(yi)(yi)旦損失(shi)(shi)(shi),輕(qing)則造成(cheng)(cheng)研究(jiu)(jiu)進(jin)(jin)(jin)度(du)(du)被(bei)耽(dan)擱,重(zhong)(zhong)(zhong)(zhong)則造成(cheng)(cheng)研究(jiu)(jiu)完全失(shi)(shi)(shi)敗(bai)!代(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)能(neng)(neng)(neng)夠(gou)充分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)保(bao)(bao)證(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)功,從(cong)而(er)防(fang)(fang)止(zhi)(zhi)了(le)(le)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)損失(shi)(shi)(shi)。當(dang)(dang)然(ran),樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)質(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)功的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前提(ti)(ti)(ti)(ti)(ti)(ti)(ti),而(er)且樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)質(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)只(zhi)(zhi)有(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)自己才能(neng)(neng)(neng)控(kong)制(zhi)(zhi)(zhi)和(he)(he)(he)(he)(he)(he)(he)(he)保(bao)(bao)證(zheng)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)原(yuan)(yuan)理(li)(li)(li)(li)(li)(li)??生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he),又稱(cheng)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he),是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)利用(yong)(yong)(yong)(yong)(yong)(yong)特(te)(te)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)成(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)、動(dong)(dong)力學(xue)(xue)及(ji)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)反(fan)應(ying)等(deng)技(ji)(ji)術(shu)制(zhi)(zhi)(zhi)備(bei)(bei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he),用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)(li)(li)(li)(li)(li)學(xue)(xue)、病(bing)理(li)(li)(li)(li)(li)(li)學(xue)(xue)、藥理(li)(li)(li)(li)(li)(li)學(xue)(xue)等(deng)領域中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)特(te)(te)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)或(huo)(huo)(huo)(huo)(huo)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)物(wu)(wu)(wu)(wu)(wu)質(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)包括底(di)(di)物(wu)(wu)(wu)(wu)(wu)、酶(mei)(mei)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)、探針等(deng)多個(ge)(ge)(ge)組(zu)(zu)(zu)成(cheng)(cheng)部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen),其(qi)(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)＊常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)底(di)(di)物(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)法(fa)(fa)(fa)(fa)。底(di)(di)物(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)法(fa)(fa)(fa)(fa)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)原(yuan)(yuan)理(li)(li)(li)(li)(li)(li)。在(zai)(zai)(zai)該過(guo)(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),底(di)(di)物(wu)(wu)(wu)(wu)(wu)與酶(mei)(mei)在(zai)(zai)(zai)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)膜上(shang)(shang)(shang)結合(he)形(xing)成(cheng)(cheng)基(ji)質(zhi)酶(mei)(mei),進(jin)(jin)(jin)而(er)形(xing)成(cheng)(cheng)底(di)(di)物(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)復(fu)合(he)物(wu)(wu)(wu)(wu)(wu)。隨著時(shi)(shi)(shi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)推移,底(di)(di)物(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)復(fu)合(he)物(wu)(wu)(wu)(wu)(wu)逐漸(jian)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)解(jie),釋放出分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)小的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)物(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)大(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)未(wei)完全消(xiao)化(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)物(wu)(wu)(wu)(wu)(wu)。未(wei)完全消(xiao)化(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)物(wu)(wu)(wu)(wu)(wu)可(ke)(ke)以(yi)(yi)(yi)被(bei)一(yi)(yi)(yi)(yi)(yi)系(xi)列的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)代(dai)(dai)謝,同(tong)(tong)(tong)時(shi)(shi)(shi)底(di)(di)物(wu)(wu)(wu)(wu)(wu)、酶(mei)(mei)和(he)(he)(he)(he)(he)(he)(he)(he)代(dai)(dai)謝產物(wu)(wu)(wu)(wu)(wu)都(dou)(dou)會(hui)對(dui)復(fu)合(he)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)穩(wen)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)產生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)影響(xiang),從(cong)而(er)影響(xiang)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結果(guo)(guo)(guo)(guo)(guo)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)可(ke)(ke)以(yi)(yi)(yi)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)型(xing)(xing)(xing)有(you)(you)(you)(you)哪些?? ?生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗(yan)原(yuan)(yuan)理(li)(li)(li)(li)(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)基(ji)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)反(fan)應(ying),理(li)(li)(li)(li)(li)(li)論(lun)上(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)(bu)受(shou)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)種(zhong)屬及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)型(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)制(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通常(chang)(chang)(chang)只(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)型(xing)(xing)(xing),不(bu)(bu)(bu)(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)種(zhong)屬。但是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)某(mou)(mou)些指(zhi)(zhi)標(biao)(biao)在(zai)(zai)(zai)不(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)種(zhong)屬或(huo)(huo)(huo)(huo)(huo)類(lei)(lei)型(xing)(xing)(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方(fang)式(shi)(shi)不(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)。因(yin)(yin)(yin)此(ci)(ci)(ci),具體(ti)(ti)(ti)(ti)(ti)情(qing)況請參見說明(ming)書(shu)或(huo)(huo)(huo)(huo)(huo)與技(ji)(ji)術(shu)支持聯(lian)(lian)系(xi)確(que)(que)認。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)般(ban)(ban)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)求:1)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)言(yan)(yan),樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)＊-好(hao)無溶血(xue)、脂血(xue)、黃-疸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現象(xiang)。組(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)(xu)將(jiang)(jiang)血(xue)液(ye)(ye)(ye)(ye)(ye)洗(xi)凈(jing)(jing)擦(ca)干(gan)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)(zai)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)稱(cheng)重(zhong)(zhong)(zhong)(zhong)處(chu)(chu)(chu)(chu)理(li)(li)(li)(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)處(chu)(chu)(chu)(chu)理(li)(li)(li)(li)(li)(li)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)＊-好(hao)盡(jin)快進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且保(bao)(bao)存(cun)(cun)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)盒(he)(he)(he)(he)(he)(he)(he)(he)之(zhi)上(shang)(shang)(shang),待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)(xu)澄(cheng)(cheng)(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),若(ruo)(ruo)不(bu)(bu)(bu)(bu)(bu)(bu)澄(cheng)(cheng)(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),需(xu)(xu)(xu)(xu)(xu)離(li)(li)(li)心(xin)(xin)(xin)(xin)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特(te)(te)殊(shu)指(zhi)(zhi)標(biao)(biao)除外(wai))。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)值(zhi)應(ying)在(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)線(xian)性(xing)(xing)(xing)(xing)(xing)(xing)范圍(wei)(wei)內,若(ruo)(ruo)超(chao)(chao)(chao)過(guo)(guo)(guo)(guo)(guo)線(xian)性(xing)(xing)(xing)(xing)(xing)(xing)范圍(wei)(wei),需(xu)(xu)(xu)(xu)(xu)稀釋后(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)(zai)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若(ruo)(ruo)低于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范圍(wei)(wei),需(xu)(xu)(xu)(xu)(xu)增加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)濃(nong)度(du)(du)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。微(wei)(wei)(wei)(wei)(wei)(wei)(wei) 量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang) 分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen) 析(xi)(xi)(xi)(xi)(xi) 法(fa)(fa)(fa)(fa)? ?????????微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(Microanalysis)在(zai)(zai)(zai)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，其(qi)(qi)(qi)(qi)(qi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)重(zhong)(zhong)(zhong)(zhong)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)為(wei)(wei)1-10mg的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)方(fang)法(fa)(fa)(fa)(fa)稱(cheng)為(wei)(wei)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)。有(you)(you)(you)(you)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)和(he)(he)(he)(he)(he)(he)(he)(he)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)之(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)，定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)常(chang)(chang)(chang)采用(yong)(yong)(yong)(yong)(yong)(yong)點(dian)滴反(fan)應(ying)和(he)(he)(he)(he)(he)(he)(he)(he)顯(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)結晶反(fan)應(ying)等(deng)靈敏(min)度(du)(du)較(jiao)高的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)方(fang)法(fa)(fa)(fa)(fa)，定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)重(zhong)(zhong)(zhong)(zhong)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)、容(rong)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)及(ji)儀(yi)器(qi)(qi)(qi)(qi)等(deng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)方(fang)法(fa)(fa)(fa)(fa)。在(zai)(zai)(zai)以(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)重(zhong)(zhong)(zhong)(zhong)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)類(lei)(lei)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)還(huan)有(you)(you)(you)(you)半(ban)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)和(he)(he)(he)(he)(he)(he)(he)(he)超(chao)(chao)(chao)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)等(deng)。微(wei)(wei)(wei)(wei)(wei)(wei)(wei) 量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang) 和(he)(he)(he)(he)(he)(he)(he)(he) 超(chao)(chao)(chao) 微(wei)(wei)(wei)(wei)(wei)(wei)(wei) 量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang) 分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen) 析(xi)(xi)(xi)(xi)(xi)????????微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)、超(chao)(chao)(chao)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)和(he)(he)(he)(he)(he)(he)(he)(he)常(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)主要(yao)(yao)(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)差(cha)(cha)別是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)不(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)。一(yi)(yi)(yi)(yi)(yi)般(ban)(ban)稱(cheng)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)在(zai)(zai)(zai)0.01克~0.001克者(zhe)(zhe)為(wei)(wei)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)，小于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)0.001克者(zhe)(zhe)為(wei)(wei)超(chao)(chao)(chao)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)。正(zheng)(zheng)(zheng)因(yin)(yin)(yin)為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)很(hen)(hen)少(shao)，所以(yi)(yi)(yi)在(zai)(zai)(zai)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)操作(zuo)(zuo)上(shang)(shang)(shang)也和(he)(he)(he)(he)(he)(he)(he)(he)常(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)很(hen)(hen)不(bu)(bu)(bu)(bu)(bu)(bu)相(xiang)同(tong)(tong)(tong)。例(li)(li)如(ru)(ru)(ru)(ru)(ru)沉(chen)(chen)淀物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)溶解(jie)度(du)(du)、指(zhi)(zhi)示劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)誤(wu)差(cha)(cha)等(deng)對(dui)常(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)影響(xiang)可(ke)(ke)以(yi)(yi)(yi)忽略(lve)，而(er)在(zai)(zai)(zai)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)(he)(he)(he)超(chao)(chao)(chao)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)時(shi)(shi)(shi)就會(hui)引起(qi)很(hen)(hen)大(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)誤(wu)差(cha)(cha)。尤(you)其(qi)(qi)(qi)(qi)(qi)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)超(chao)(chao)(chao)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)求更(geng)嚴(yan)格(ge)，須(xu)考慮到(dao)溶液(ye)(ye)(ye)(ye)(ye)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)身在(zai)(zai)(zai)空(kong)氣(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)蒸(zheng)(zheng)發(fa)，因(yin)(yin)(yin)此(ci)(ci)(ci)在(zai)(zai)(zai)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)滴定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)時(shi)(shi)(shi)必緩(huan)在(zai)(zai)(zai)潮濕的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)空(kong)氣(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)。????????一(yi)(yi)(yi)(yi)(yi)般(ban)(ban)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)用(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)器(qi)(qi)(qi)(qi)皿(min)(min)都(dou)(dou)很(hen)(hen)小，按(an)(an)(an)(an)(an)(an)(an)其(qi)(qi)(qi)(qi)(qi)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)求的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)不(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)有(you)(you)(you)(you)各(ge)種(zhong)不(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)形(xing)狀。各(ge)種(zhong)儀(yi)器(qi)(qi)(qi)(qi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)法(fa)(fa)(fa)(fa)經(jing)(jing)過(guo)(guo)(guo)(guo)(guo)適當(dang)(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)改進(jin)(jin)(jin)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)，可(ke)(ke)以(yi)(yi)(yi)直接應(ying)用(yong)(yong)(yong)(yong)(yong)(yong)在(zai)(zai)(zai)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)上(shang)(shang)(shang)。超(chao)(chao)(chao)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)由(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)太少(shao)，因(yin)(yin)(yin)此(ci)(ci)(ci)必須(xu)在(zai)(zai)(zai)顯(xian)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)鏡下進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)所有(you)(you)(you)(you)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)操作(zuo)(zuo)(如(ru)(ru)(ru)(ru)(ru)沉(chen)(chen)淀和(he)(he)(he)(he)(he)(he)(he)(he)濡定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)等(deng))，某(mou)(mou)些儀(yi)器(qi)(qi)(qi)(qi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(如(ru)(ru)(ru)(ru)(ru)電(dian)位滴定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)，電(dian)解(jie)和(he)(he)(he)(he)(he)(he)(he)(he)電(dian)導(dao)等(deng))，也可(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)來(lai)(lai)作(zuo)(zuo)為(wei)(wei)超(chao)(chao)(chao)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)之(zhi)用(yong)(yong)(yong)(yong)(yong)(yong)。????????微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)在(zai)(zai)(zai)研究(jiu)(jiu)工作(zuo)(zuo)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)用(yong)(yong)(yong)(yong)(yong)(yong)得(de)極(ji)廣(guang)。尤(you)其(qi)(qi)(qi)(qi)(qi)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)在(zai)(zai)(zai)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)稀有(you)(you)(you)(you)元(yuan)素化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)研究(jiu)(jiu)時(shi)(shi)(shi)，在(zai)(zai)(zai)解(jie)決(jue)地(di)球化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)同(tong)(tong)(tong)題，研究(jiu)(jiu)某(mou)(mou)些稀有(you)(you)(you)(you)元(yuan)素礦(kuang)物(wu)(wu)(wu)(wu)(wu)粗成(cheng)(cheng)及(ji)其(qi)(qi)(qi)(qi)(qi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)配規(gui)律時(shi)(shi)(shi)，往(wang)往(wang)只(zhi)(zhi)能(neng)(neng)(neng)得(de)到(dao)極(ji)少(shao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)，在(zai)(zai)(zai)這(zhe)選(xuan)(xuan)種(zhong)情(qing)況下一(yi)(yi)(yi)(yi)(yi)般(ban)(ban)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)常(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)法(fa)(fa)(fa)(fa)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)無法(fa)(fa)(fa)(fa)進(jin)(jin)(jin)行(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，只(zhi)(zhi)有(you)(you)(you)(you)靠(kao)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)法(fa)(fa)(fa)(fa)來(lai)(lai)解(jie)決(jue)。另(ling)外(wai)，在(zai)(zai)(zai)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)用(yong)(yong)(yong)(yong)(yong)(yong)更(geng)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)普遍(bian)果(guo)(guo)(guo)(guo)(guo)糖(tang)-1,6-二磷酸(suan)(suan)醛縮酶(mei)(mei)(FBA)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)科(ke)研實(shi)驗(yan),不(bu)(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)臨(lin)床。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)意(yi)(yi)(yi)義:輔(fu)酶(mei)(mei)NAD(H)廣(guang)泛存(cun)(cun)在(zai)(zai)(zai)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)、植物(wu)(wu)(wu)(wu)(wu)、微(wei)(wei)(wei)(wei)(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)(he)培(pei)養(yang)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),NAD+是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)糖(tang)酵解(jie)(EMP)和(he)(he)(he)(he)(he)(he)(he)(he)三(san)羧酸(suan)(suan)循(xun)環(TCA)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)(yao)(yao)(yao)(yao)(yao)(yao)氫(qing)受(shou)體(ti)(ti)(ti)(ti)(ti),生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?NADH?經(jing)(jing)呼吸電(dian)子(zi)鏈(ETC)傳遞把電(dian)子(zi)交給(gei)氧(yang),在(zai)(zai)(zai)合(he)成(cheng)(cheng)?ATP?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)(tong)(tong)時(shi)(shi)(shi),形(xing)成(cheng)(cheng)大(da)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?ROS,同(tong)(tong)(tong)時(shi)(shi)(shi)?NADH?再(zai)(zai)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)為(wei)(wei)?NAD+。糖(tang)、脂、蛋(dan)(dan)(dan)白(bai)(bai)質(zhi)三(san)大(da)代(dai)(dai)謝物(wu)(wu)(wu)(wu)(wu)質(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)解(jie)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)氧(yang)化(hua)(hua)(hua)(hua)(hua)反(fan)應(ying)絕大(da)部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)通過(guo)(guo)(guo)(guo)(guo)這(zhe)一(yi)(yi)(yi)(yi)(yi)體(ti)(ti)(ti)(ti)(ti)系(xi)完成(cheng)(cheng)。NAD(H)含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)(he)(he)(he)?NADH/NAD+比(bi)(bi)(bi)值(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)高低可(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)評價糖(tang)酵解(jie)和(he)(he)(he)(he)(he)(he)(he)(he)?TCA?循(xun)環的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)強弱。較(jiao)高的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)NAD(H)及(ji)?NADH/NAD+比(bi)(bi)(bi)值(zhi)說明(ming)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)呼吸耗氧(yang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)較(jiao)高,處(chu)(chu)(chu)(chu)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)過(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)(hua)狀態。此(ci)(ci)(ci)外(wai),NADH/NAD+比(bi)(bi)(bi)值(zhi)升高也可(ke)(ke)抑(yi)制(zhi)(zhi)(zhi)糖(tang)酵解(jie)和(he)(he)(he)(he)(he)(he)(he)(he)?TCA?循(xun)環。另(ling)外(wai),NAD+降解(jie)產物(wu)(wu)(wu)(wu)(wu)對(dui)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)信號傳導(dao)、代(dai)(dai)謝和(he)(he)(he)(he)(he)(he)(he)(he)基(ji)因(yin)(yin)(yin)表達等(deng)具有(you)(you)(you)(you)重(zhong)(zhong)(zhong)(zhong)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)調控(kong)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)原(yuan)(yuan)理(li)(li)(li)(li)(li)(li):分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別用(yong)(yong)(yong)(yong)(yong)(yong)酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)和(he)(he)(he)(he)(he)(he)(he)(he)堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)?NADH,NADH?通過(guo)(guo)(guo)(guo)(guo)?PMS?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)遞氫(qing)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong),還(huan)原(yuan)(yuan)氧(yang)化(hua)(hua)(hua)(hua)(hua)型(xing)(xing)(xing)噻唑藍(MTT)為(wei)(wei)甲(jia)瓚,在(zai)(zai)(zai)?570nm?下檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)吸光(guang)(guang)(guang)(guang)(guang)(guang)值(zhi);而(er)?NAD+可(ke)(ke)被(bei)乙(yi)醇(chun)脫氫(qing)酶(mei)(mei)還(huan)原(yuan)(yuan)為(wei)(wei)?NADH,進(jin)(jin)(jin)一(yi)(yi)(yi)(yi)(yi)步采用(yong)(yong)(yong)(yong)(yong)(yong)?MTT?還(huan)原(yuan)(yuan)法(fa)(fa)(fa)(fa)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。需(xu)(xu)(xu)(xu)(xu)自備(bei)(bei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)儀(yi)器(qi)(qi)(qi)(qi)和(he)(he)(he)(he)(he)(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)品(pin)(pin)(pin)(pin)(pin):酶(mei)(mei)標(biao)(biao)儀(yi)、臺式(shi)(shi)離(li)(li)(li)心(xin)(xin)(xin)(xin)機(ji)、移液(ye)(ye)(ye)(ye)(ye)器(qi)(qi)(qi)(qi)、96?孔板(ban)、研缽、冰(bing)(bing)(bing)(bing)和(he)(he)(he)(he)(he)(he)(he)(he)蒸(zheng)(zheng)餾水(shui)(shui)(shui)(shui)。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)(zu)成(cheng)(cheng)和(he)(he)(he)(he)(he)(he)(he)(he)配制(zhi)(zhi)(zhi):酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping),4℃保(bao)(bao)存(cun)(cun);堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping),4℃保(bao)(bao)存(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi):液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?10 mL×1?瓶(ping),4℃保(bao)(bao)存(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)二:液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?3 mL×1?瓶(ping),4℃保(bao)(bao)存(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)三(san):粉(fen)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping),-20?℃保(bao)(bao)存(cun)(cun),用(yong)(yong)(yong)(yong)(yong)(yong)時(shi)(shi)(shi)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?3mL?蒸(zheng)(zheng)餾水(shui)(shui)(shui)(shui),混(hun)勻(yun)(yun)(yun),用(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)(bu)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)存(cun)(cun)一(yi)(yi)(yi)(yi)(yi)周;試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)四:粉(fen)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping),4?℃保(bao)(bao)存(cun)(cun),用(yong)(yong)(yong)(yong)(yong)(yong)時(shi)(shi)(shi)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?3mL?蒸(zheng)(zheng)餾水(shui)(shui)(shui)(shui),混(hun)勻(yun)(yun)(yun),用(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)(bu)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)存(cun)(cun)一(yi)(yi)(yi)(yi)(yi)周;試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)五:液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?3.6mL×1?瓶(ping),4?℃保(bao)(bao)存(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu)(liu):液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?30mL×1?瓶(ping),4?℃保(bao)(bao)存(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)七:液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping),4?℃保(bao)(bao)存(cun)(cun)。NAD+和(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):1?血(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)血(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(mL):酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(mL)為(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(li)(建(jian)議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)?0.1mL?血(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?1mL?酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)?5min(蓋(gai)(gai)緊(jin)(jin)(jin)(jin),以(yi)(yi)(yi)防(fang)(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻(yun)(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)血(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(mL):堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(mL)為(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(li)(建(jian)議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)?0.1mL血(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?1mL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)?5min(蓋(gai)(gai)緊(jin)(jin)(jin)(jin),以(yi)(yi)(yi)防(fang)(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?500μL?酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻(yun)(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。2?組(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)組(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)質(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(g):酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(mL)為(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(li)(建(jian)議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)?0.1g?組(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?1mL酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)研磨(mo),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)?5min(蓋(gai)(gai)緊(jin)(jin)(jin)(jin),以(yi)(yi)(yi)防(fang)(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻(yun)(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)組(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)質(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(g):堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(mL)為(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(li)(建(jian)議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)?0.1g?組(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?1mL堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)研磨(mo),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)?5min(蓋(gai)(gai)緊(jin)(jin)(jin)(jin),以(yi)(yi)(yi)防(fang)(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?500μL?酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻(yun)(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3?細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先(xian)收集(ji)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)到(dao)離(li)(li)(li)心(xin)(xin)(xin)(xin)管(guan)(guan)(guan)內,棄上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)):酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(mL)為(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(li)(建(jian)議(yi)(yi)?500?萬(wan)(wan)細(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?1mL?酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),超(chao)(chao)(chao)聲(sheng)(sheng)波(bo)破碎(冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu),功率20%或(huo)(huo)(huo)(huo)(huo)?200W,超(chao)(chao)(chao)聲(sheng)(sheng)?3s,間(jian)隔?10s,重(zhong)(zhong)(zhong)(zhong)復(fu)?30?次(ci)(ci)(ci)),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)?5min(蓋(gai)(gai)緊(jin)(jin)(jin)(jin),以(yi)(yi)(yi)防(fang)(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻(yun)(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先(xian)收集(ji)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)到(dao)離(li)(li)(li)心(xin)(xin)(xin)(xin)管(guan)(guan)(guan)內,棄上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)):堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(mL)為(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(li)(建(jian)議(yi)(yi)?500?萬(wan)(wan)細(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?1mL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),超(chao)(chao)(chao)聲(sheng)(sheng)波(bo)破碎(冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu),功率20%或(huo)(huo)(huo)(huo)(huo)?200W,超(chao)(chao)(chao)聲(sheng)(sheng)?3s,間(jian)隔?10s,重(zhong)(zhong)(zhong)(zhong)復(fu)?30?次(ci)(ci)(ci)),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)?5min(蓋(gai)(gai)緊(jin)(jin)(jin)(jin),以(yi)(yi)(yi)防(fang)(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?500μL?酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻(yun)(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。充分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)混(hun)勻(yun)(yun)(yun),靜置(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)?5min?后(hou)(hou)(hou)(hou)(hou)(hou)(hou),20000g,25℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?5min,棄上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),沉(chen)(chen)淀中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru):混(hun)勻(yun)(yun)(yun),取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?200μL?轉(zhuan)移至微(wei)(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)石(shi)英比(bi)(bi)(bi)色(se)皿(min)(min)或(huo)(huo)(huo)(huo)(huo)?96?孔板(ban)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),570nm?下讀取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)對(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)吸光(guang)(guang)(guang)(guang)(guang)(guang)值(zhi)?A1?和(he)(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)吸光(guang)(guang)(guang)(guang)(guang)(guang)值(zhi)?A2,計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)△A=A2-A1。注(zhu)(zhu)意(yi)(yi)(yi)事項:1、如(ru)(ru)(ru)(ru)(ru)果(guo)(guo)(guo)(guo)(guo)一(yi)(yi)(yi)(yi)(yi)次(ci)(ci)(ci)性(xing)(xing)(xing)(xing)(xing)(xing)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)數(shu)較(jiao)多,可(ke)(ke)將(jiang)(jiang)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)、二、三(san)和(he)(he)(he)(he)(he)(he)(he)(he)四按(an)(an)(an)(an)(an)(an)(an)比(bi)(bi)(bi)例(li)(li)配成(cheng)(cheng)混(hun)合(he)液(ye)(ye)(ye)(ye)(ye)。2、對(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)步驟的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別:對(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)完試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)、二、三(san)、四和(he)(he)(he)(he)(he)(he)(he)(he)五后(hou)(hou)(hou)(hou)(hou)(hou)(hou)必須(xu)馬上(shang)(shang)(shang)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu)(liu);測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)完試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)、二、三(san)、四和(he)(he)(he)(he)(he)(he)(he)(he)五后(hou)(hou)(hou)(hou)(hou)(hou)(hou)必須(xu)反(fan)應(ying)?20min?后(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu)(liu)。3、反(fan)應(ying)過(guo)(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)注(zhu)(zhu)意(yi)(yi)(yi)避(bi)光(guang)(guang)(guang)(guang)(guang)(guang)。4、若(ruo)(ruo)?NAD+測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0302,NADH?測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0222,說明(ming)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)輔(fu)酶(mei)(mei)含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)較(jiao)低,已低于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian)(xian),可(ke)(ke)做如(ru)(ru)(ru)(ru)(ru)下調整(zheng):(1)將(jiang)(jiang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)避(bi)光(guang)(guang)(guang)(guang)(guang)(guang)靜置(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)間(jian)?20min?延長到(dao)?60min;(2)在(zai)(zai)(zai)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)階段增加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang),即取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?0.2g?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)或(huo)(huo)(huo)(huo)(huo)?0.2mL?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?1mL?提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)。5、由(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)每一(yi)(yi)(yi)(yi)(yi)個(ge)(ge)(ge)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)設一(yi)(yi)(yi)(yi)(yi)個(ge)(ge)(ge)對(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan),本(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)?100?管(guan)(guan)(guan)保(bao)(bao)證(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)?48?個(ge)(ge)(ge)?NAD+或(huo)(huo)(huo)(huo)(huo)?NADH.NAD+和(he)(he)(he)(he)(he)(he)(he)(he)?NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan):(一(yi)(yi)(yi)(yi)(yi)) NAD+含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)標(biao)(biao)準條件(jian)下的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回(hui)歸曲線(xian)為(wei)(wei)?y = 0.1475x + 0.0302,R2 = 0.9978;其(qi)(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)△A,x?為(wei)(wei)?NAD+濃(nong)度(du)(du)?nmol/mL1、血(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)=[(△A -0.0302)÷0.1475×V1) ]÷(V3×V1÷V2)=135.6×(△A -0.0302)2、組(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)、細(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(1)按(an)(an)(an)(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋(dan)(dan)(dan)白(bai)(bai)濃(nong)度(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+(nmol/mg prot)=[(△A -0.0302)÷0.1475×V1)]÷(V1×Cpr)= 6.8×(△A -0.0302)÷Cpr(2)按(an)(an)(an)(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)鮮(xian)(xian)重(zhong)(zhong)(zhong)(zhong)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+(nmol/g?鮮(xian)(xian)重(zhong)(zhong)(zhong)(zhong))= [(△A -0.0302)÷0.1475×V1)]÷(W×V1÷V2)= 13.6×(△A -0.0302)÷W(3)按(an)(an)(an)(an)(an)(an)(an)細(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)密度(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+(nmol/104 cell)=[(△A -0.0302)÷0.1475×V1)]÷(500×V1÷V2)=0.027×(△A -0.0302)(二)NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)標(biao)(biao)準條件(jian)下的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回(hui)歸曲線(xian)為(wei)(wei)?y = 0.1404x + 0.0222,R2 = 0.9976;其(qi)(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)△A,x?為(wei)(wei)?NADH?濃(nong)度(du)(du)?nmol/mL1、血(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)= [ (△A -0.0222)÷0.1404×V1) ]÷(V3×V1÷V2)= 142.5×(△A -0.0222)2、組(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)、細(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(1)按(an)(an)(an)(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋(dan)(dan)(dan)白(bai)(bai)濃(nong)度(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH (nmol/mg prot)= [ (△A -0.0222)÷0.1404×V1) ]÷(V1×Cpr)= 7.1×(△A -0.0222)÷Cpr(2)按(an)(an)(an)(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)鮮(xian)(xian)重(zhong)(zhong)(zhong)(zhong)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH (nmol/g?鮮(xian)(xian)重(zhong)(zhong)(zhong)(zhong))=[(△A -0.0222)÷0.1404×V1) ]÷(W×V1÷V2)= 14.2×(△A -0.0222)÷W(3)按(an)(an)(an)(an)(an)(an)(an)細(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)密度(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH (nmol/104 cell)= [ (△A -0.0222)÷0.1404×V1) ]÷(500×V1÷V2)=0.028×(△A -0.0222)V1:加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)反(fan)應(ying)體(ti)(ti)(ti)(ti)(ti)系(xi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji),0.02mL;V2:加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji),2mL;V3:加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)血(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji):0.1mL;Cpr:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋(dan)(dan)(dan)白(bai)(bai)質(zhi)濃(nong)度(du)(du),mg/mL; W:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)質(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang),g;500:細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)總數(shu),500?萬(wan)(wan)。注(zhu)(zhu)?意(yi)(yi)(yi):?檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian)(xian)為(wei)(wei)?0.1nmol/mL?或(huo)(huo)(huo)(huo)(huo)?0.1nmol/g?鮮(xian)(xian)重(zhong)(zhong)(zhong)(zhong)?或(huo)(huo)(huo)(huo)(huo)?0.001nmol/mg prot

PeproTech于1988年創(chuang)立于美國新澤西(xi)州，經過(guo)26年的發展，已(yi)成為世界(jie)(jie)上＊大的重組(zu)細胞因子和(he)蛋白(bai)、相(xiang)關抗體及ELISA試劑(ji)盒生(sheng)產(chan)(chan)(chan)商之一。PeproTech公司已(yi)開發出2,000多(duo)種產(chan)(chan)(chan)品，其精良的技術保證了(le)產(chan)(chan)(chan)品的高質量、高可靠性(xing)和(he)高穩定(ding)性(xing)。 Peprotech的產(chan)(chan)(chan)品具(ju)有很高的性(xing)價(jia)比，在(zai)世界(jie)(jie)上眾多(duo)高水平的實驗室和(he)研究機構中(zhong)廣泛(fan)使用。截至(zhi)2013年底，已(yi)被13,000篇SCI文獻引用。

賽默飛世爾(er)科技(ji)公司(Thermo Fisher Scientific)是一(yi)家總部位(wei)于(yu)馬薩諸塞州沃爾(er)瑟姆的美國(guo)科技(ji)服(fu)務供應商，在1956年由數個公司合并而成，其(qi)主要客戶類型(xing)包括醫藥(yao)和生物公司，醫院和臨(lin)床診斷(duan)實(shi)驗室(shi)，大(da)學、科研院所和政(zheng)府機構，以(yi)及環境與工業過程(cheng)控制(zhi)裝備制(zhi)造商等，為其(qi)提(ti)供用于(yu)研究、分(fen)析(xi)、發現和診斷(duan)的分(fen)析(xi)儀器(qi)(qi)、設備、試(shi)(shi)劑和耗材(cai)、軟件和服(fu)務。Thermo Scientific能夠提(ti)供一(yi)整套包括高(gao)端分(fen)析(xi)儀器(qi)(qi)、實(shi)驗室(shi)裝備、軟件、服(fu)務、耗材(cai)和試(shi)(shi)劑在內的實(shi)驗室(shi)綜合解決方案(an)。

圣克(ke)(ke)魯斯生(sheng)(sheng)物技術在生(sheng)(sheng)物醫藥(yao)研究市場的發(fa)展中(zhong)處(chu)于＊＊＊＊地位。在過去(qu)的30年中(zhong),本公(gong)司已把重點放(fang)在不斷發(fa)展的研究單克(ke)(ke)隆抗體、生(sheng)(sheng)物化學(xue)、實驗(yan)室器具(ju)和CRISPR產品。

 Tocris Bioscience是(shi)一家專賣生命科(ke)(ke)學研究chemicals, peptides and antibodies的知名(ming)品(pin)牌，其產(chan)品(pin)也(ye)廣為(wei)各大藥廠、大學、研究機(ji)構，超過五萬名(ming)科(ke)(ke)學研究者所(suo)采用。Tocris bioscience是(shi)位(wei)于(yu)英國(guo)布里斯托(tuo)爾（Bristol）的高品(pin)質試劑(ji)提供商，共有2000多種(zhong)產(chan)品(pin)，主要集中在神(shen)經(jing)科(ke)(ke)學和(he)信號傳導領域，產(chan)品(pin)類型包括小(xiao)分(fen)子、多肽、抗體、配體和(he)化合物篩(shai)選(xuan)文庫等，主要產(chan)品(pin)包括GPCR ligands，神(shen)經(jing)傳遞素(su)，離(li)子通道(dao)調控(kong)劑(ji)，信號通路(lu)抑制劑(ji)等，這些產(chan)品(pin)被(bei)廣泛選(xuan)擇性(xing)地用于(yu)阻斷或激活生物學通路(lu)。

 R&D細(xi)胞因子，R&D ELISA試劑盒(he)，RD試劑產(chan)品(pin)描述(shu)：我公司專業(ye)代理R&D產(chan)品(pin)，產(chan)品(pin)多樣，涉及細(xi)胞因子，生(sheng)物試劑，ELISA試劑盒(he)。保證原(yuan)裝，價格，貨(huo)期快，服務(wu)好(hao)！

QIAGEN，是全球(qiu)＊＊的(de)(de)(de)樣品(pin)制備和(he)檢測技術(shu)供應(ying)商。樣品(pin)制備技術(shu)用(yong)來分(fen)離和(he)處(chu)理從(cong)血液或組織等(deng)生物樣品(pin)中的(de)(de)(de)DNA，RNA和(he)蛋白(bai)質，檢測技術(shu)使這些分(fen)離的(de)(de)(de)分(fen)子，在隨后的(de)(de)(de)分(fen)析(xi)中顯現(xian)，如特定(ding)病毒(du)的(de)(de)(de)DNA。其使命是使客戶在生命科學(xue)，應(ying)用(yong)測試，制藥，分(fen)子診(zhen)斷等(deng)方面實現(xian)卓越(yue)的(de)(de)(de)成就和(he)突破。

代理品牌(pai)

Invitrogen是全(quan)(quan)球(qiu)(qiu)＊＊的(de)(de)生(sheng)(sheng)命(ming)技術(shu)公(gong)司(si)，公(gong)司(si)創(chuang)立于1987年(nian)，總部位于美(mei)國加利福尼亞州卡爾斯巴德(de)。主要(yao)為(wei)全(quan)(quan)球(qiu)(qiu)從事生(sheng)(sheng)命(ming)科學(xue)研(yan)究(jiu)(jiu)的(de)(de)學(xue)術(shu)、政府研(yan)究(jiu)(jiu)機(ji)構(gou)、醫藥和生(sheng)(sheng)物(wu)(wu)(wu)(wu)技術(shu)公(gong)司(si)提(ti)(ti)供生(sheng)(sheng)物(wu)(wu)(wu)(wu)技術(shu)產(chan)品(pin)(pin)和服(fu)務。Invitrogen將研(yan)發力量集中(zhong)在包括功能基(ji)因組(zu)(zu)、蛋白組(zu)(zu)學(xue)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)信息(xi)學(xue)和細胞生(sheng)(sheng)物(wu)(wu)(wu)(wu)學(xue)等(deng)生(sheng)(sheng)命(ming)科學(xue)研(yan)究(jiu)(jiu)領域內的(de)(de)突破性(xing)創(chuang)新，向全(quan)(quan)球(qiu)(qiu)各主要(yao)實驗室提(ti)(ti)供在研(yan)究(jiu)(jiu)中(zhong)所需(xu)要(yao)的(de)(de)新技術(shu)、新產(chan)品(pin)(pin)及服(fu)務。 Invitrogen旗下包括Invitrogen、Gibco、MolecularProbes、Dynal、Biosource、Caltag、Zymed、Panvera、InforMax、BioReliance等(deng)眾多行業＊＊＊＊。為(wei)分子生(sheng)(sheng)物(wu)(wu)(wu)(wu)、疾病研(yan)究(jiu)(jiu)，藥物(wu)(wu)(wu)(wu)研(yan)發和生(sheng)(sheng)物(wu)(wu)(wu)(wu)制品(pin)(pin)生(sheng)(sheng)產(chan)等(deng)提(ti)(ti)供全(quan)(quan)面的(de)(de)技術(shu)和服(fu)務。

Cell Signaling Technology (CST) 是美國＊＊的(de)(de)生(sheng)物公(gong)司(si)和(he)細胞信號轉導研(yan)究的(de)(de)＊＊，提(ti)供特色的(de)(de)信號轉導檢測用抗體(ti)及磷酸化(hua)、乙酰化(hua)、甲基化(hua)、泛素化(hua)抗體(ti)、ELISA試(shi)劑盒、細胞因子、化(hua)學試(shi)劑、激(ji)酶等產品。CST一家總(zong)部位于(yu)美國馬(ma)薩(sa)諸(zhu)塞州(zhou)丹弗斯(si)的(de)(de)私營(ying)公(gong)司(si),擁有專業的(de)(de)生(sheng)產和(he)研(yan)發(fa)隊伍，致力于(yu)提(ti)供全球(qiu)高品質創新型(xing)研(yan)究產品，以加速生(sheng)物學認知

代(dai)理(li)品(pin)牌(pai) 

AnaSpec于1993年在(zai)美國成(cheng)立，是一家由多肽合(he)成(cheng)、熒(ying)光標(biao)記(ji)、抗體、樹脂合(he)成(cheng)專家團隊(dui)組(zu)成(cheng)的生(sheng)物(wu)企(qi)業，為全(quan)世界(jie)的科研工作者提(ti)供(gong)全(quan)套(tao)的蛋白質組(zu)學(xue)研究解決方案。

代理品牌

AbcamAbcam位于(yu)英國的(de)劍橋科(ke)學園，成(cheng)立于(yu)1998年，為生(sheng)命科(ke)學研究提(ti)供嚴格驗證的(de)抗體,試(shi)劑,蛋(dan)白(bai),試(shi)劑盒。超過11萬種優質高效的(de)蛋(dan)白(bai)研究工具,提(ti)供數據表、多(duo)項驗證并附有客戶評(ping)價,現貨(huo)速達,幫您更快完成(cheng)實(shi)驗。

BioVision. Inc. 位于美(mei)國(guo)加州，是世界上＊＊＊＊專著(zhu)于凋(diao)亡(wang)和細胞信(xin)號研(yan)究(jiu)的(de)公司(si)。其產品質(zhi)優(you)、價廉、包裝(zhuang)使用方便。BioVision致力于為(wei)研(yan)究(jiu)者(zhe)們提供(gong)＊好(hao)、＊新的(de)研(yan)究(jiu)工具而(er)不(bu)斷擴(kuo)大(da)產品及服務質(zhi)量(liang)。該公司(si)提供(gong)用于檢(jian)測早、中、晚期凋(diao)亡(wang)的(de)試(shi)劑(ji)，可以檢(jian)測凋(diao)亡(wang)發生(sheng)的(de)不(bu)同區(qu)域(yu)，包括：線粒體、胞漿、胞膜及胞核。

Cayman ChemicalCayman Chemical在位于(yu)密西根安娜堡（Ann Arbor, Michigan）66,000平方英尺的工(gong)廠(chang)生產(chan)(chan)并制作近6000多(duo)種不同的化合物、抗(kang)體及分析試(shi)劑(ji)盒。向全球科學(xue)(xue)工(gong)作者提供(gong)多(duo)研(yan)究領域的生化、免疫(yi)試(shi)劑(ji)和分析試(shi)劑(ji)盒，其(qi)產(chan)(chan)品(pin)被(bei)廣泛應用于(yu)腫瘤(liu)、氧化氮(dan)、神(shen)經(jing)學(xue)(xue)、凋(diao)亡、氧化性損傷(shang)、內分泌學(xue)(xue)等不同研(yan)究領域。Cayman Chemical可提供(gong)用于(yu)檢(jian)測的特色(se)試(shi)劑(ji)盒，也提供(gong)多(duo)種高質量試(shi)劑(ji)

Enzo Life Sciences成立于1976年(nian)，總部設于美國紐約，在研發(fa)、生產、銷售(shou)生物研究(jiu)試劑方(fang)面(mian)擁有40多年(nian)的成功經驗(yan)，涉及(ji)靶(ba)標鑒定及(ji)驗(yan)證、高通量分(fen)(fen)析(xi)、基因表達分(fen)(fen)析(xi)、蛋(dan)白質檢測及(ji)細(xi)胞分(fen)(fen)析(xi)等相關(guan)領(ling)域，可提供蛋(dan)白、抗體、多肽、小分(fen)(fen)子、標簽探針(zhen)染料及(ji)試劑盒等一萬余(yu)種產品。