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 嘔(ou)(ou)吐(tu)(tu)毒(du)素(su)(su)為(wei)白(bai)(bai)(bai)(bai)色針(zhen)狀(zhuang)結晶，熔點為(wei)151 ~153℃（醋酸乙(yi)基石油）。不(bu)飽和(he)酮基的存在使得其(qi)在短波紫外(wai)下(xia)(xia)有吸收(shou)(shou)峰(feng)，但與(yu)許多(duo)其(qi)它物(wu)質在此處(chu)的紫外(wai)吸收(shou)(shou)峰(feng)相重疊，屬非特征性(xing)(xing)的。紫外(wai)輻射(she)下(xia)(xia)不(bu)顯熒光。DON易溶(rong)于極(ji)性(xing)(xing)的溶(rong)劑(ji)如水、甲(jia)醇、乙(yi)醇、乙(yi)腈、丙酮和(he)乙(yi)酸乙(yi)酯，不(bu)溶(rong)于正已烷(wan)、丁(ding)醇、石油醚(mi)。鼠(shu)(shu)抗(kang)(kang)嘔(ou)(ou)吐(tu)(tu)毒(du)素(su)(su)單(dan)(dan)克(ke)隆抗(kang)(kang)體(ti)說(shuo)明書(shu)【中文(wen)(wen)名(ming)稱(cheng)】鼠(shu)(shu)抗(kang)(kang)嘔(ou)(ou)吐(tu)(tu)毒(du)素(su)(su)單(dan)(dan)克(ke)隆抗(kang)(kang)體(ti)【英文(wen)(wen)名(ming)稱(cheng)】Mouse Anti-vomiting Toxin Monoclonal Antibody【貨    號(hao)】DM-Ab0180【來    源】Balb/c 小鼠(shu)(shu)【亞    型】IgG  【物(wu)理性(xing)(xing)狀(zhuang)】液體(ti)，避免(mian)(mian)反(fan)復凍(dong)(dong)融(rong)【ELISA 效價(jia)(jia)】 0.005M PBS 體(ti)系下(xia)(xia)1:200000 以(yi)上(shang) 【IC50 】30ppb（μg/Kg）【緩(huan)沖(chong)液】0.01M PBS PH7.4  不(bu)加防腐劑(ji)【儲存及有效期(qi)】-20℃以(yi)下(xia)(xia)3 年，避免(mian)(mian)反(fan)復凍(dong)(dong)融(rong)【相關產(chan)品】鼠(shu)(shu)抗(kang)(kang)黃(huang)曲霉毒(du)素(su)(su)B1單(dan)(dan)克(ke)隆抗(kang)(kang)體(ti)；鼠(shu)(shu)抗(kang)(kang)黃(huang)曲霉毒(du)素(su)(su)總量單(dan)(dan)克(ke)隆抗(kang)(kang)體(ti)；鼠(shu)(shu)抗(kang)(kang)黃(huang)曲霉毒(du)素(su)(su)M1單(dan)(dan)克(ke)隆抗(kang)(kang)體(ti) 嘔(ou)(ou)吐(tu)(tu)毒(du)素(su)(su)偶(ou)聯(lian)免(mian)(mian)疫(yi)全(quan)抗(kang)(kang)原說(shuo)明書(shu)【中文(wen)(wen)名(ming)稱(cheng)】嘔(ou)(ou)吐(tu)(tu)毒(du)素(su)(su)偶(ou)聯(lian)全(quan)抗(kang)(kang)原【英文(wen)(wen)名(ming)稱(cheng)】Vomitoxin-Protein Conjugate【貨    號(hao)】DM-Ag0180【蛋白(bai)(bai)(bai)(bai)載(zai)體(ti)】牛(niu)血清(qing)白(bai)(bai)(bai)(bai)蛋白(bai)(bai)(bai)(bai)/卵清(qing)白(bai)(bai)(bai)(bai)蛋白(bai)(bai)(bai)(bai)/匙孔血藍蛋白(bai)(bai)(bai)(bai)BSA/OVA/KLH【物(wu)理性(xing)(xing)狀(zhuang)】液體(ti)，避免(mian)(mian)反(fan)復凍(dong)(dong)融(rong)【ELISA效價(jia)(jia)】 0.005M PBS 體(ti)系下(xia)(xia)1:30000以(yi)上(shang)【免(mian)(mian)疫(yi)層析效價(jia)(jia)】 1:40以(yi)上(shang)，偶(ou)聯(lian)比(bi)大于1:60 【免(mian)(mian)疫(yi)原偶(ou)聯(lian)位點】3-OH √ /15-0H【蛋白(bai)(bai)(bai)(bai)濃(nong)度】5.0 mg/ml【緩(huan)沖(chong)液】0.01M PBS PH7.4無(wu)防腐劑(ji)【儲存條件】-20℃以(yi)下(xia)(xia)【有效期(qi)】2年，避免(mian)(mian)反(fan)復凍(dong)(dong)融(rong) 

菌(jun)(jun)(jun)(jun)種(zhong)說明 提示：請(qing)嚴格按(an)(an)(an)照(zhao)說明書操(cao)作,如(ru)未按(an)(an)(an)說明書操(cao)作，本(ben)中心不(bu)(bu)承擔售(shou)后。 本(ben)中心目前有(you)標準菌(jun)(jun)(jun)(jun)種(zhong)（斜(xie)面(mian)、甘 油菌(jun)(jun)(jun)(jun)、穿(chuan)(chuan)(chuan)刺(ci)菌(jun)(jun)(jun)(jun)和(he)凍(dong)(dong)干(gan)(gan)粉四(si)種(zhong)形(xing)式）、基因(yin)工(gong)程菌(jun)(jun)(jun)(jun)種(zhong)（斜(xie)面(mian)、甘油 菌(jun)(jun)(jun)(jun)和(he)穿(chuan)(chuan)(chuan)刺(ci)菌(jun)(jun)(jun)(jun)三種(zhong)形(xing)式）、食用(yong)(yong)益生(sheng)(sheng)菌(jun)(jun)(jun)(jun)和(he)工(gong) 業(ye)發酵(jiao)菌(jun)(jun)(jun)(jun)種(zhong)，基本(ben)涵蓋了環境、工(gong)業(ye)、農業(ye)、分析(xi)、食品、藥(yao) 用(yong)(yong)真(zhen)菌(jun)(jun)(jun)(jun)等各類(lei)生(sheng)(sheng)產和(he)科(ke)研微生(sheng)(sheng)物(wu)(wu)(wu)。 一 菌(jun)(jun)(jun)(jun)種(zhong)簡(jian)介 菌(jun)(jun)(jun)(jun)種(zhong)名(ming)稱(cheng) 牙齦卟啉單胞菌(jun)(jun)(jun)(jun) 編(bian)號 ATCC33277 規(gui)格 培(pei)(pei)(pei)養(yang)皿 菌(jun)(jun)(jun)(jun)種(zhong)主要應(ying)(ying)用(yong)(yong) 二 保(bao)(bao)存(cun)(cun)(cun)條件 斜(xie)面(mian)、穿(chuan)(chuan)(chuan)刺(ci)菌(jun)(jun)(jun)(jun)和(he)凍(dong)(dong)干(gan)(gan)粉可(ke)常溫(wen)運(yun)輸，但應(ying)(ying)在4-10度長(chang)(chang)期保(bao)(bao)存(cun)(cun)(cun)。甘油菌(jun)(jun)(jun)(jun)可(ke)常溫(wen)運(yun)輸，但應(ying)(ying)在-80度長(chang)(chang)期 保(bao)(bao)存(cun)(cun)(cun)。微生(sheng)(sheng)物(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)種(zhong)應(ying)(ying)保(bao)(bao)存(cun)(cun)(cun)于低溫(wen)、清(qing)潔(jie)和(he)干(gan)(gan)燥的地方(fang)，室(shi)溫(wen)放置(zhi)時間(jian)過長(chang)(chang)會導致菌(jun)(jun)(jun)(jun)種(zhong)衰退。 三 培(pei)(pei)(pei)養(yang)條件 培(pei)(pei)(pei)養(yang)基 血瓊脂平(ping)板 培(pei)(pei)(pei)養(yang)溫(wen)度 37 度 培(pei)(pei)(pei)養(yang)時間(jian) 2-5 天(tian) 培(pei)(pei)(pei)養(yang)條件 嚴格厭氧(yang) 四(si) 活化步驟 培(pei)(pei)(pei)養(yang)皿培(pei)(pei)(pei)養(yang)物(wu)(wu)(wu)首次傳代，先(xian)用(yong)(yong)75%酒精將斜(xie)面(mian)硅(gui)(gui)膠塞處擦拭，然后酒精燈(deng)旁(pang)燒(shao)一下消(xiao)毒，然后開啟硅(gui)(gui)膠塞， 用(yong)(yong)接種(zhong)針(zhen)（細(xi)菌(jun)(jun)(jun)(jun)類(lei)）或(huo)接種(zhong)鏟（霉菌(jun)(jun)(jun)(jun)類(lei)）取一點菌(jun)(jun)(jun)(jun)種(zhong)，然后接種(zhong)到新的斜(xie)面(mian)試管上(shang)（劃線涂布都可(ke)以）。 也可(ke)以接種(zhong)到液體培(pei)(pei)(pei)養(yang)基中做搖菌(jun)(jun)(jun)(jun)培(pei)(pei)(pei)養(yang)。剩余(yu)的菌(jun)(jun)(jun)(jun)種(zhong)可(ke)以按(an)(an)(an)照(zhao)保(bao)(bao)存(cun)(cun)(cun)條件放到相(xiang)應(ying)(ying)條件保(bao)(bao)存(cun)(cun)(cun)。如(ru)果有(you)不(bu)(bu)明白 之(zhi)處，應(ying)(ying)先(xian)與我(wo)中心聯系，避免不(bu)(bu)必要的損失(4009681786,微信：)。 五 注(zhu)意事項(xiang) 1 微生(sheng)(sheng)物(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)種(zhong)應(ying)(ying)保(bao)(bao)存(cun)(cun)(cun)于低溫(wen)、清(qing)潔(jie)和(he)干(gan)(gan)燥的地方(fang)，室(shi)溫(wen)放置(zhi)時間(jian)過長(chang)(chang)會導致菌(jun)(jun)(jun)(jun)種(zhong)衰退； 2 菌(jun)(jun)(jun)(jun)種(zhong)操(cao)作在無菌(jun)(jun)(jun)(jun)條件下進行，接種(zhong)完畢應(ying)(ying)該滅(mie)菌(jun)(jun)(jun)(jun)再做丟棄處理； 3 斜(xie)面(mian)菌(jun)(jun)(jun)(jun)種(zhong)和(he)穿(chuan)(chuan)(chuan)刺(ci)菌(jun)(jun)(jun)(jun)種(zhong)保(bao)(bao)存(cun)(cun)(cun)時間(jian)通常 為 3-6 個月，應(ying)(ying)根據菌(jun)(jun)(jun)(jun)種(zhong)狀況及時結轉；凍(dong)(dong)干(gan)(gan)粉保(bao)(bao)存(cun)(cun)(cun)時間(jian)通常是2-25年(nian)；甘油菌(jun)(jun)(jun)(jun)保(bao)(bao)存(cun)(cun)(cun)時間(jian)為2-5年(nian)

大鼠血管內皮細胞(bao)生(sheng)(sheng)長因(yin)(yin)子A(VEGF-A)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)文名稱大鼠血管內皮細胞(bao)生(sheng)(sheng)長因(yin)(yin)子A(VEGF-A)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)規格(ge)96T/48T樣(yang)(yang)(yang)本(ben)(ben)(ben)類型血清、血漿(jiang)、細胞(bao)上清液(ye)(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)(ye)、體(ti)液(ye)(ye)(ye)(ye)(ye)、灌洗(xi)(xi)(xi)液(ye)(ye)(ye)(ye)(ye)等使(shi)(shi)用(yong)范(fan)圍科研實(shi)(shi)驗(yan)，不(bu)(bu)得(de)用(yong)于(yu)臨床(chuang)診斷(duan)反(fan)應(ying)時間(jian)1-5h試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)2-8℃有(you)效期6個月(yue)貨(huo)期現(xian)貨(huo)【操作(zuo)步(bu)驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)與(yu)(yu)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)：在(zai)(zai)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被(bei)板(ban)上設標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)10孔(kong)(kong)(kong)，在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，然(ran)后(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)中(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)；然(ran)后(hou)(hou)(hou)從第(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)中(zhong)(zhong)各(ge)(ge)取(qu)(qu)100μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)和(he)第(di)(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)，再(zai)(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)；然(ran)后(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)和(he)第(di)(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)中(zhong)(zhong)先各(ge)(ge)取(qu)(qu)50μl棄(qi)掉(diao)，再(zai)(zai)(zai)各(ge)(ge)取(qu)(qu)50μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)中(zhong)(zhong)，再(zai)(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50ul，混(hun)(hun)勻(yun)；混(hun)(hun)勻(yun)后(hou)(hou)(hou)從第(di)(di)(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)中(zhong)(zhong)各(ge)(ge)取(qu)(qu)50μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)中(zhong)(zhong)，再(zai)(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)后(hou)(hou)(hou)從第(di)(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)取(qu)(qu)50μl加(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)(di)九(jiu)、第(di)(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)中(zhong)(zhong)，再(zai)(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)后(hou)(hou)(hou)從第(di)(di)(di)(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)中(zhong)(zhong)各(ge)(ge)取(qu)(qu)50μl棄(qi)掉(diao)。（稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)各(ge)(ge)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)量(liang)(liang)都為(wei)50μl，濃(nong)(nong)度(du)(du)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)為(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)：分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)設空(kong)白(bai)(bai)孔(kong)(kong)(kong)（空(kong)白(bai)(bai)對(dui)(dui)照孔(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)及(ji)(ji)(ji)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，其余各(ge)(ge)步(bu)操作(zuo)相同(tong)）、待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)。在(zai)(zai)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被(bei)板(ban)上待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)中(zhong)(zhong)先加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)40μl，然(ran)后(hou)(hou)(hou)再(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)＊終(zhong)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)度(du)(du)為(wei)5倍(bei)）。加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)將樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)于(yu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)孔(kong)(kong)(kong)底部，盡(jin)量(liang)(liang)不(bu)(bu)觸及(ji)(ji)(ji)孔(kong)(kong)(kong)壁，輕(qing)輕(qing)晃動混(hun)(hun)勻(yun)。3. 溫(wen)(wen)育：用(yong)封板(ban)膜(mo)封板(ban)后(hou)(hou)(hou)置(zhi)37℃溫(wen)(wen)育30分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。4. 配液(ye)(ye)(ye)(ye)(ye)：將30（48T的(de)(de)(de)(de)(de)(de)(de)20倍(bei)）倍(bei)濃(nong)(nong)縮(suo)洗(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)用(yong)蒸餾水30（48T的(de)(de)(de)(de)(de)(de)(de)20倍(bei)）倍(bei)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)備用(yong)。5. 洗(xi)(xi)(xi)滌(di)(di)(di)：小(xiao)心揭掉(diao)封板(ban)膜(mo)，棄(qi)去(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)，甩干，每(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)滿(man)洗(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)，靜(jing)置(zhi)30秒后(hou)(hou)(hou)棄(qi)去(qu)，如此重復(fu)5次，拍干。6. 加(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)：每(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)50μl，空(kong)白(bai)(bai)孔(kong)(kong)(kong)除(chu)外。7. 溫(wen)(wen)育：操作(zuo)同(tong)3。8. 洗(xi)(xi)(xi)滌(di)(di)(di)：操作(zuo)同(tong)5。9. 顯(xian)色(se)(se)(se)(se)：每(mei)孔(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)顯(xian)色(se)(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)(ji)A50μl，再(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)顯(xian)色(se)(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)(ji)B50μl，輕(qing)輕(qing)震蕩混(hun)(hun)勻(yun)，37℃避(bi)(bi)光顯(xian)色(se)(se)(se)(se)15分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong). 10. 終(zhong)止：每(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止反(fan)應(ying)（此時藍色(se)(se)(se)(se)立轉(zhuan)黃(huang)色(se)(se)(se)(se)）。11. 測(ce)(ce)(ce)(ce)(ce)定(ding)：以(yi)(yi)空(kong)白(bai)(bai)空(kong)調(diao)零，450nm波(bo)長依序測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)各(ge)(ge)孔(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)吸光度(du)(du)（OD值(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)(ce)(ce)定(ding)應(ying)在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)內進行(xing)(xing)。【注(zhu)意事(shi)項】1． 試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)從冷藏環(huan)境(jing)中(zhong)(zhong)取(qu)(qu)出應(ying)在(zai)(zai)室溫(wen)(wen)平(ping)(ping)衡15-30分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)方可(ke)使(shi)(shi)用(yong)，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被(bei)板(ban)開(kai)封后(hou)(hou)(hou)如未用(yong)完，板(ban)條應(ying)裝(zhuang)入(ru)(ru)密(mi)封袋(dai)中(zhong)(zhong)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)。2． 濃(nong)(nong)洗(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)可(ke)能會(hui)有(you)結(jie)(jie)晶析(xi)出，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)時可(ke)在(zai)(zai)水浴中(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)溫(wen)(wen)助溶(rong)，洗(xi)(xi)(xi)滌(di)(di)(di)時不(bu)(bu)影響結(jie)(jie)果(guo)。3． 各(ge)(ge)步(bu)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)均應(ying)使(shi)(shi)用(yong)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)器，并經常校對(dui)(dui)其準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性，以(yi)(yi)避(bi)(bi)免(mian)試(shi)(shi)(shi)驗(yan)誤差(cha)。一(yi)(yi)次加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)時間(jian)＊好控制在(zai)(zai)5分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內，如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)數(shu)(shu)(shu)量(liang)(liang)多，推薦(jian)使(shi)(shi)用(yong)排(pai)槍(qiang)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)。4． 請(qing)(qing)(qing)每(mei)次測(ce)(ce)(ce)(ce)(ce)定(ding)的(de)(de)(de)(de)(de)(de)(de)同(tong)時做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)，＊好做復(fu)孔(kong)(kong)(kong)。如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)中(zhong)(zhong)待測(ce)(ce)(ce)(ce)(ce)物(wu)質含量(liang)(liang)過(guo)高(gao)(gao)（樣(yang)(yang)(yang)本(ben)(ben)(ben)OD值(zhi)(zhi)(zhi)大于(yu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)），請(qing)(qing)(qing)先用(yong)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)定(ding)倍(bei)數(shu)(shu)(shu)（n倍(bei)）后(hou)(hou)(hou)再(zai)(zai)(zai)測(ce)(ce)(ce)(ce)(ce)定(ding)，計(ji)(ji)算時請(qing)(qing)(qing)＊后(hou)(hou)(hou)乘以(yi)(yi)總稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)（×n×5）。5． 封板(ban)膜(mo)只(zhi)限一(yi)(yi)次性使(shi)(shi)用(yong)，以(yi)(yi)避(bi)(bi)免(mian)交(jiao)(jiao)叉(cha)污染。6． 底物(wu)請(qing)(qing)(qing)避(bi)(bi)光保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)。7． 嚴格(ge)按照說明(ming)書的(de)(de)(de)(de)(de)(de)(de)操作(zuo)進行(xing)(xing)，試(shi)(shi)(shi)驗(yan)結(jie)(jie)果(guo)判定(ding)必須(xu)以(yi)(yi)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀數(shu)(shu)(shu)為(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun).8． 所(suo)(suo)有(you)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)，洗(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)和(he)各(ge)(ge)種廢棄(qi)物(wu)都應(ying)按傳染物(wu)處理(li)。9． 本(ben)(ben)(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)不(bu)(bu)同(tong)批(pi)(pi)號組(zu)分(fen)(fen)(fen)不(bu)(bu)得(de)混(hun)(hun)用(yong)。10. 如與(yu)(yu)英文說明(ming)書有(you)異，以(yi)(yi)英文說明(ming)書為(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)。【計(ji)(ji)算】 以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)的(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)為(wei)橫(heng)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)縱(zong)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)，    在(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上繪出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)，根據樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)OD      值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)查(cha)出相應(ying)的(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)；再(zai)(zai)(zai)乘以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)      倍(bei)數(shu)(shu)(shu)；或用(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)的(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)與(yu)(yu)OD值(zhi)(zhi)(zhi)計(ji)(ji)算出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)      準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)的(de)(de)(de)(de)(de)(de)(de)直線(xian)回歸方程式，將樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)      代入(ru)(ru)方程式，計(ji)(ji)算出樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)，再(zai)(zai)(zai)乘以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)      倍(bei)數(shu)(shu)(shu)，即為(wei)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際(ji)濃(nong)(nong)度(du)(du)。                   【試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)組(zu)成(cheng)】試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)組(zu)成(cheng)48孔(kong)(kong)(kong)配置(zhi)96孔(kong)(kong)(kong)配置(zhi)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)說明(ming)書1份(fen)1份(fen)密(mi)封袋(dai)1個1個封板(ban)膜(mo)12孔(kong)(kong)(kong)×4條2片（96）2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)微孔(kong)(kong)(kong)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)1×4812孔(kong)(kong)(kong)×8條2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)顯(xian)色(se)(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)顯(xian)色(se)(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)終(zhong)止液(ye)(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)注(zhu)意：使(shi)(shi)用(yong)前請(qing)(qing)(qing)檢(jian)查(cha)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和(he)數(shu)(shu)(shu)量(liang)(liang)與(yu)(yu)表(biao)格(ge)是否一(yi)(yi)致。【精密(mi)度(du)(du)】精密(mi)度(du)(du)用(yong)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)定(ding)值(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)變異系(xi)數(shu)(shu)(shu)CV 表(biao)示。CV（%） = SD/mean×100批(pi)(pi)內差(cha)：取(qu)(qu)同(tong)批(pi)(pi)次試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)對(dui)(dui)低、中(zhong)(zhong)、高(gao)(gao)值(zhi)(zhi)(zhi)定(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)本(ben)(ben)(ben)進行(xing)(xing)定(ding)量(liang)(liang)檢(jian)測(ce)(ce)(ce)(ce)(ce)，每(mei)份(fen)樣(yang)(yang)(yang)本(ben)(ben)(ben)連續測(ce)(ce)(ce)(ce)(ce)定(ding)20 次，分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)計(ji)(ji)算不(bu)(bu)同(tong)濃(nong)(nong)度(du)(du)樣(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)平(ping)(ping)均值(zhi)(zhi)(zhi)及(ji)(ji)(ji)SD 值(zhi)(zhi)(zhi)。批(pi)(pi)內差(cha)： CV<9%批(pi)(pi)間(jian)差(cha)：選取(qu)(qu)3 個不(bu)(bu)同(tong)批(pi)(pi)次的(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)對(dui)(dui)低、中(zhong)(zhong)、高(gao)(gao)值(zhi)(zhi)(zhi)定(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)本(ben)(ben)(ben)進行(xing)(xing)定(ding)量(liang)(liang)測(ce)(ce)(ce)(ce)(ce)定(ding)，每(mei)個樣(yang)(yang)(yang)本(ben)(ben)(ben)使(shi)(shi)用(yong)同(tong)一(yi)(yi)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)重復(fu)測(ce)(ce)(ce)(ce)(ce)定(ding)10 次，分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)計(ji)(ji)算不(bu)(bu)同(tong)濃(nong)(nong)度(du)(du)樣(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)平(ping)(ping)均值(zhi)(zhi)(zhi)及(ji)(ji)(ji)SD 值(zhi)(zhi)(zhi)。批(pi)(pi)間(jian)差(cha)： CV<11% 【特異性】本(ben)(ben)(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)識別(bie)(bie)(bie)(bie)天然(ran)和(he)重組(zu)微生(sheng)(sheng)物(wu)硝酸鹽還原酶(mei)(mei)（Nar），經檢(jian)測(ce)(ce)(ce)(ce)(ce)與(yu)(yu)其它相似物(wu)質無明(ming)顯(xian)交(jiao)(jiao)叉(cha)反(fan)應(ying)。由于(yu)受(shou)到(dao)技術及(ji)(ji)(ji)樣(yang)(yang)(yang)本(ben)(ben)(ben)來(lai)源的(de)(de)(de)(de)(de)(de)(de)限制，不(bu)(bu)可(ke)能完成(cheng)所(suo)(suo)有(you)相關或相似物(wu)質交(jiao)(jiao)叉(cha)反(fan)應(ying)檢(jian)測(ce)(ce)(ce)(ce)(ce)，因(yin)(yin)此本(ben)(ben)(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)有(you)可(ke)能與(yu)(yu)未經檢(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)其它物(wu)質有(you)交(jiao)(jiao)叉(cha)反(fan)應(ying)。 【穩定(ding)性】試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)在(zai)(zai)有(you)效期內務必按推薦(jian)溫(wen)(wen)度(du)(du)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)，活性降低率小(xiao)于(yu)5%。為(wei)減小(xiao)外部因(yin)(yin)素對(dui)(dui)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)破壞前后(hou)(hou)(hou)檢(jian)測(ce)(ce)(ce)(ce)(ce)值(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)影響，實(shi)(shi)驗(yan)室的(de)(de)(de)(de)(de)(de)(de)環(huan)境(jing)條件需盡(jin)量(liang)(liang)保(bao)(bao)(bao)(bao)持(chi)一(yi)(yi)致，尤其是實(shi)(shi)驗(yan)室內溫(wen)(wen)度(du)(du)、濕度(du)(du)及(ji)(ji)(ji)溫(wen)(wen)育條件。其次由同(tong)一(yi)(yi)實(shi)(shi)驗(yan)員來(lai)進行(xing)(xing)操作(zuo)可(ke)減少(shao)人為(wei)誤差(cha)。

CTCF Monoclonal Antibody Catalog No  DM-00964 Isotype IgG Reactivity Human;Monkey Applications WB;ELISA Gene Name CTCF Protein Name Transcriptional repressor CTCFImmunogen Purified recombinant fragment of human CTCF expressed in E. Coli. Specificity CTCF Monoclonal Antibody detects endogenous levels of CTCF protein. Formulation Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol. Source Monoclonal, Mouse Purification Affinity purificationDilution Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. Concentration 1 mg/ml Purity ≥90% Storage Stability -20°C/1 year Synonyms CTCF; Transcriptional repressor CTCF; 11-zinc finger protein; CCCTC-binding factor; CTCFL paralog Observed Band Cell Pathway Nucleus, nucleoplasm . Chromosome . Chromosome, centromere . May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase. Tissue Specificity Ubiquitous. Absent in primary spermatocytes. Function function:Transcriptional repressor binding to promoters of vertebrate c-myc gene. Also binds to the PLK and PIM1 promoters. May prevent the access of transcriptional activators to enhancers. Also acts as a transcriptional activator of APP. Involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Seems to act as tumor suppressor.,similarity:Belongs to the CTCF zinc-finger protein family.,similarity:Contains 11 C2H2-type zinc fingers.,subunit:Interacts with CHD8.,tissue specificity:Ubiquitous. Absent in primary spermatocytes.,Background This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combination

ZN143 rabbit pAb Catalog No DM-09315 Isotype IgGReactivity Human;Mouse;Rat Applications WB Gene Name ZNF143 SBF STAF Protein Name Zinc finger protein 143 (SPH-binding factor) (Selenocysteine tRNA gene transcription-activating factor) (hStaf) Immunogen Synthesized peptide derived from human ZN143 AA range: 518-568 Specificity This antibody detects endogenous levels of human ZN143 Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgG&nbsp;Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. Dilution WB 1:1000-2000 Concentration 1 mg/ml Purity ≥90% Storage Stability -20°C/1 year Synonyms Observed Band Cell Pathway Nucleus . Tissue Specificity Expressed in all tissues tested, with the strongest expression in ovary. Function Background matters needing attention Avoid repeated freezing and thawing! Usage suggestions This product can be used in immunological reaction related experiments. For more information, please consult technical personnel.

Elf-1 Polyclonal Antibody Catalog No DM-01689 Isotype IgGReactivity Human Applications WB;IHC;IF;ELISA Gene Name ELF1 Protein Name ETS-related transcription factor Elf-1 Immunogen The antiserum was produced against synthesized peptide derived from human ELF1. AA range:537-586 Specificity Elf-1 Polyclonal Antibody detects endogenous levels of Elf-1 protein. Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgG Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Dilution Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. Concentration 1 mg/ml Purity ≥90% Storage Stability -20°C/1 year Synonyms ELF1; ETS-related transcription factor Elf-1; E74-like factor 1 Observed Band 67kD Cell Pathway Nucleus. Tissue Specificity In fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis. Function function:Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,subunit:Binds to the underphosphorylated form of RB. May interact with other transcription factors in order to regulate specific genes. Interacts with RUNX1.,tissue specificity:In fetal tissue, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it

Kinase experiment [1]: Preparation Method Prior to use, the [8-14C] adenosine was checked for purity using isocratic HPLC elution. The assay incubation mixture contained 0.4 IU of enzyme in 50 L. The metabolites were separated by thin-layer chromatography. The radioactivity was quantitated by cutting the plastic backed TLC plates and placing them in scintillation vials, and counting in a Packard 2000 CA scintillation counter Applications IC50: 0.15 (HIV-1, A012 isolate), 0.20 μM (HIV-1, A018 isolate). 3-Deazaadenosine (hydrochloride) is an inhibitor of S-adenosylhomocysteine hydrolase, with a Ki of 3.9 μM. Cell experiment [2]: Cell lines Mouse macrophage RAW 264.7 Preparation Method RAW 264.7 cells were pretreated with or without 3-Deazaadenosine (100 μMM) for 1 h, and stimulated by the addition of LPS (1 μg/ml). After incubation for 1 h, the cells were washed, and p65 was recovered by immunoprecipitation with anti-p65 and protein A-Sepharose. Reaction Conditions 0-100 μM 3-Deazaadenosine for 1 hour Applications 3-Deazaadenosine (1-100 ?M) inhibits LPS-induced expression of TNF-α mRNA, increases DNA binding activity of NF-κB, and causes proteolytic degradation of IκBα, but Not IκBβ in RAW 264.7 cells. 3-Deazaadenosine (100 μM) enhances nuclear translocation of NF-κB, but blocks LPS-induced NF-κB transcriptional activity, and such inhibition is augmented by the addition of homocysteine. Animal experiment [3]: Animal models Male, healthy Sprague–Dawley rats (300?50 g) Preparation Method Animals and balloon injury: rats were fed for 5 days prior and 14 days after the balloon injury with standard chow containing c3Ado at a concentration of 10 mg/kg 3-Deazaadenosine body weight. Dosage form 10 mg/kg 3-Deazaadenosine for 5 days prior and 14 days after the balloon injury Applications 3-deazaadenosine (c3Ado) inhibits atherogenesis in mice. Sprague Dawley rats underwent balloon angioplasty. C3Ado was administered orally, starting 5 days prior to the balloon injury and continued for 2 weeks. Fourteen days afte

 小(xiao)(xiao)(xiao)鼠(shu)胰(yi)島素(su)(su)(su)(su)(su)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)生(sheng)(sheng)長因子(zi) 1（IGF-1）實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)原(yuan)理   試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)一(yi)步(bu)(bu)夾心法酶(mei)(mei)(mei)聯(lian)免(mian)疫吸(xi)(xi)附試(shi)(shi)(shi)驗(yan)(yan)（ELISA）。往預(yu)先(xian)(xian)包(bao)被脂肪酸結(jie)合蛋(dan)白(bai)(bai)(bai)(bai)1(FABP1)捕獲抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包(bao)被微孔(kong)(kong)(kong)中，依(yi)次(ci)加(jia)(jia)入(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)，經過(guo)(guo)(guo)(guo)(guo)溫(wen)育(yu)并(bing)徹底(di)洗(xi)(xi)(xi)滌。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)底(di)物(wu)(wu)(wu)TMB顯(xian)色(se)(se)(se)(se)，TMB在(zai)(zai)過(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)(hua)(hua)下(xia)轉化(hua)(hua)(hua)(hua)成藍(lan)色(se)(se)(se)(se)，并(bing)在(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)下(xia)轉化(hua)(hua)(hua)(hua)成＊終的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)(se)(se)(se)。顏色(se)(se)(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂肪酸結(jie)合蛋(dan)白(bai)(bai)(bai)(bai)1(FABP1)呈正(zheng)相(xiang)(xiang)(xiang)關(guan)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)在(zai)(zai)450nm 波長下(xia)測(ce)(ce)定吸(xi)(xi)光度(du)(du)(du)（OD 值(zhi)），計(ji)算(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)。保(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)條(tiao)(tiao)件(jian)(jian)及(ji)有(you)(you)(you)效期(qi)(qi)本(ben)(ben)(ben)試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)體(ti)(ti)(ti)外定量(liang)(liang)(liang)(liang)檢(jian)(jian)(jian)測(ce)(ce)血(xue)(xue)清(qing)(qing)、血(xue)(xue)漿、組織勻(yun)(yun)漿及(ji)相(xiang)(xiang)(xiang)關(guan)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)中脂肪酸結(jie)合蛋(dan)白(bai)(bai)(bai)(bai)1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)量(liang)(liang)(liang)(liang)。有(you)(you)(you)效期(qi)(qi)：6個(ge)月(yue)保(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)條(tiao)(tiao)件(jian)(jian)：2-8℃小(xiao)(xiao)(xiao)鼠(shu)胰(yi)島素(su)(su)(su)(su)(su)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)生(sheng)(sheng)長因子(zi) 1（IGF-1）一(yi)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集(ji)(ji)(ji)(ji)及(ji)保(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun) 1.血(xue)(xue)清(qing)(qing)：全血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)請(qing)(qing)于(yu)(yu)室溫(wen)放(fang)置(zhi)2小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)或(huo)(huo)4℃過(guo)(guo)(guo)(guo)(guo)一(yi)晚后(hou)(hou)(hou)于(yu)(yu)1000 x g離(li)心20分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，取(qu)(qu)上(shang)(shang)清(qing)(qing)即可(ke)檢(jian)(jian)(jian)測(ce)(ce)，或(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放(fang)于(yu)(yu)-20℃或(huo)(huo)-80℃保(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)，但(dan)應(ying)(ying)避(bi)(bi)免(mian)反(fan)(fan)(fan)復凍(dong)(dong)融(rong)(rong)(rong)。 2.血(xue)(xue)漿：可(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)肝(gan)素(su)(su)(su)(su)(su)作(zuo)為(wei)(wei)(wei)(wei)抗(kang)(kang)(kang)(kang)凝劑(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)采(cai)集(ji)(ji)(ji)(ji)后(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)于(yu)(yu)2 - 8° C 1000 x g離(li)心15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放(fang)于(yu)(yu)-20℃或(huo)(huo)-80℃保(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)，但(dan)應(ying)(ying)避(bi)(bi)免(mian)反(fan)(fan)(fan)復凍(dong)(dong)融(rong)(rong)(rong)。 3.細胞(bao)培養物(wu)(wu)(wu)上(shang)(shang)清(qing)(qing)或(huo)(huo)其(qi)它生(sheng)(sheng)物(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)：1000 x g離(li)心20分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，取(qu)(qu)上(shang)(shang)清(qing)(qing)即可(ke)檢(jian)(jian)(jian)測(ce)(ce)，或(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放(fang)于(yu)(yu)-20℃或(huo)(huo)-80℃保(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)，但(dan)應(ying)(ying)避(bi)(bi)免(mian)反(fan)(fan)(fan)復凍(dong)(dong)融(rong)(rong)(rong)。 注：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)溶(rong)血(xue)(xue)會影(ying)響檢(jian)(jian)(jian)測(ce)(ce)結(jie)果(guo)，因此溶(rong)血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)不(bu)(bu)宜進行(xing)此項檢(jian)(jian)(jian)測(ce)(ce)。二、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)   首(shou)先(xian)(xian)通過(guo)(guo)(guo)(guo)(guo)文獻檢(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)式了解待測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含(han)量(liang)(liang)(liang)(liang)，確(que)定適當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)(shu)。只有(you)(you)(you)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍(wei)內(nei)(nei)(nei)(nei)，檢(jian)(jian)(jian)測(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果(guo)才是準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)(guo)程(cheng)中，應(ying)(ying)做(zuo)好(hao)詳細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)錄。計(ji)算(suan)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)應(ying)(ying)再乘(cheng)以(yi)(yi)(yi)(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)：2瓶，每(mei)(mei)(mei)瓶臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)以(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至1ml，蓋(gai)好(hao)后(hou)(hou)(hou)靜置(zhi)10分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)上(shang)(shang)，然后(hou)(hou)(hou)反(fan)(fan)(fan)復顛倒/搓(cuo)動(dong)(dong)以(yi)(yi)(yi)(yi)助溶(rong)解，其(qi)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)(wei)300 pg/ml，做(zuo)系(xi)列倍(bei)(bei)(bei)比稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)直(zhi)接作(zuo)為(wei)(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)0 pg/ml，臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)。 如(ru)(ru)配(pei)(pei)(pei)(pei)制(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取(qu)(qu)0.5ml（不(bu)(bu)要(yao)少于(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)入(ru)含(han)0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中，混(hun)(hun)勻(yun)(yun)即可(ke)，其(qi)余(yu)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)以(yi)(yi)(yi)(yi)此類推。2.生(sheng)(sheng)物(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)： 臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)以(yi)(yi)(yi)(yi)生(sheng)(sheng)物(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)根(gen)(gen)(gen)(gen)據(ju)預(yu)先(xian)(xian)計(ji)算(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)100μl），實(shi)(shi)(shi)(shi)(shi)(shi)際(ji)配(pei)(pei)(pei)(pei)制(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)多(duo)配(pei)(pei)(pei)(pei)制(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl生(sheng)(sheng)物(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)加(jia)(jia)990μl生(sheng)(sheng)物(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)(pei)(pei)制(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)勻(yun)(yun)，在(zai)(zai)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)一(yi)小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)。3.辣(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)： 臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)以(yi)(yi)(yi)(yi)辣(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)根(gen)(gen)(gen)(gen)據(ju)預(yu)先(xian)(xian)計(ji)算(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)100μl），實(shi)(shi)(shi)(shi)(shi)(shi)際(ji)配(pei)(pei)(pei)(pei)制(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)多(duo)配(pei)(pei)(pei)(pei)制(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl辣(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)加(jia)(jia)990μl辣(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)(pei)(pei)制(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)勻(yun)(yun)，在(zai)(zai)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)一(yi)小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)。 操(cao)作(zuo)步(bu)(bu)驟(zou) 實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)開始(shi)前(qian)，請(qing)(qing)提前(qian)配(pei)(pei)(pei)(pei)置(zhi)好(hao)所有(you)(you)(you)試(shi)(shi)(shi)劑(ji)(ji)，試(shi)(shi)(shi)劑(ji)(ji)或(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)，均(jun)需混(hun)(hun)勻(yun)(yun)，混(hun)(hun)勻(yun)(yun)時(shi)(shi)(shi)(shi)(shi)盡(jin)量(liang)(liang)(liang)(liang)避(bi)(bi)免(mian)起(qi)泡。每(mei)(mei)(mei)次(ci)檢(jian)(jian)(jian)測(ce)(ce)都應(ying)(ying)該做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)。如(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)過(guo)(guo)(guo)(guo)(guo)高時(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)進行(xing)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)使樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)范圍(wei)。 小(xiao)(xiao)(xiao)鼠(shu)胰(yi)島素(su)(su)(su)(su)(su)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)生(sheng)(sheng)長因子(zi) 1（IGF-1）三、操(cao)作(zuo)步(bu)(bu)驟(zou)1.加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)設(she)空白(bai)(bai)(bai)(bai)孔(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)、待測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)。空白(bai)(bai)(bai)(bai)孔(kong)(kong)(kong)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)100μl，余(yu)孔(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)待測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注意不(bu)(bu)要(yao)有(you)(you)(you)氣(qi)泡，加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)于(yu)(yu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)底(di)部，盡(jin)量(liang)(liang)(liang)(liang)不(bu)(bu)觸及(ji)孔(kong)(kong)(kong)壁(bi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)晃動(dong)(dong)混(hun)(hun)勻(yun)(yun)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)上(shang)(shang)蓋(gai)或(huo)(huo)覆膜，37℃反(fan)(fan)(fan)應(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)(wei)保(bao)(bao)證(zheng)實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)結(jie)果(guo)有(you)(you)(you)效性，每(mei)(mei)(mei)次(ci)實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)請(qing)(qing)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)。2.棄去液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩干(gan)，不(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)(xi)滌。每(mei)(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)生(sheng)(sheng)物(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取(qu)(qu)1μl生(sheng)(sheng)物(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)加(jia)(jia)99μl生(sheng)(sheng)物(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)(pei)(pei)制(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)勻(yun)(yun)，在(zai)(zai)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)一(yi)小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。 3.溫(wen)育(yu)60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)，棄去孔(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩干(gan)，洗(xi)(xi)(xi)板(ban)(ban)(ban)3次(ci)，每(mei)(mei)(mei)次(ci)浸泡1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)，甩干(gan)。 4.每(mei)(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)辣(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)（同(tong)生(sheng)(sheng)物(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。 5.溫(wen)育(yu)60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)，棄去孔(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩干(gan)，洗(xi)(xi)(xi)板(ban)(ban)(ban)5次(ci)，每(mei)(mei)(mei)次(ci)浸泡1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)，甩干(gan)。 6.依(yi)序(xu)每(mei)(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)底(di)物(wu)(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)(bi)光顯(xian)色(se)(se)(se)(se)（30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)，此時(shi)(shi)(shi)(shi)(shi)肉眼可(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)3-4孔(kong)(kong)(kong)有(you)(you)(you)明(ming)顯(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)藍(lan)色(se)(se)(se)(se)，后(hou)(hou)(hou)3-4孔(kong)(kong)(kong)梯度(du)(du)(du)不(bu)(bu)明(ming)顯(xian)，即可(ke)終止(zhi)）。 7.依(yi)序(xu)每(mei)(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)終止(zhi)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，終止(zhi)反(fan)(fan)(fan)應(ying)(ying)（此時(shi)(shi)(shi)(shi)(shi)藍(lan)色(se)(se)(se)(se)立轉黃色(se)(se)(se)(se)）。終止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)入(ru)順序(xu)應(ying)(ying)盡(jin)量(liang)(liang)(liang)(liang)與(yu)底(di)物(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)入(ru)順序(xu)相(xiang)(xiang)(xiang)同(tong)。為(wei)(wei)(wei)(wei)了保(bao)(bao)證(zheng)實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性，底(di)物(wu)(wu)(wu)反(fan)(fan)(fan)應(ying)(ying)時(shi)(shi)(shi)(shi)(shi)間到后(hou)(hou)(hou)應(ying)(ying)盡(jin)快(kuai)加(jia)(jia)入(ru)終止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)。 8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)聯(lian)儀(yi)在(zai)(zai)450nm波長依(yi)序(xu)測(ce)(ce)量(liang)(liang)(liang)(liang)各孔(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光密(mi)度(du)(du)(du)（OD值(zhi)）。 在(zai)(zai)加(jia)(jia)終止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)內(nei)(nei)(nei)(nei)進行(xing)檢(jian)(jian)(jian)測(ce)(ce)。 四、試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)小(xiao)(xiao)(xiao)技(ji)巧1. 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)初次(ci)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)時(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)將(jiang)各種試(shi)(shi)(shi)劑(ji)(ji)管離(li)心數(shu)(shu)(shu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)便(bian)試(shi)(shi)(shi)劑(ji)(ji)集(ji)(ji)(ji)(ji)中到管底(di)。2. 每(mei)(mei)(mei)次(ci)實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)留一(yi)孔(kong)(kong)(kong)作(zuo)為(wei)(wei)(wei)(wei)空白(bai)(bai)(bai)(bai)調零(ling)孔(kong)(kong)(kong)，該孔(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)任何試(shi)(shi)(shi)劑(ji)(ji)，只是**加(jia)(jia)底(di)物(wu)(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)及(ji)2N H2SO4。測(ce)(ce)量(liang)(liang)(liang)(liang)時(shi)(shi)(shi)(shi)(shi)先(xian)(xian)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)此孔(kong)(kong)(kong)調OD值(zhi)至零(ling)。 3. 為(wei)(wei)(wei)(wei)防止(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸(zheng)發，試(shi)(shi)(shi)驗(yan)(yan)時(shi)(shi)(shi)(shi)(shi)將(jiang)反(fan)(fan)(fan)應(ying)(ying)板(ban)(ban)(ban)放(fang)于(yu)(yu)鋪有(you)(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密(mi)閉盒(he)(he)(he)內(nei)(nei)(nei)(nei)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)上(shang)(shang)蓋(gai)或(huo)(huo)覆膜。 4. 未使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)或(huo)(huo)者(zhe)試(shi)(shi)(shi)劑(ji)(ji)，請(qing)(qing)于(yu)(yu)2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)請(qing)(qing)依(yi)據(ju)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)置(zhi)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)勿重(zhong)(zhong)復使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)已(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)、辣(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)。 5. 建議(yi)檢(jian)(jian)(jian)測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)均(jun)設(she)雙孔(kong)(kong)(kong)測(ce)(ce)定，以(yi)(yi)(yi)(yi)保(bao)(bao)證(zheng)檢(jian)(jian)(jian)測(ce)(ce)結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性。 洗(xi)(xi)(xi)板(ban)(ban)(ban)方(fang)(fang)法 手(shou)工(gong)(gong)洗(xi)(xi)(xi)板(ban)(ban)(ban)方(fang)(fang)法：吸(xi)(xi)去（不(bu)(bu)可(ke)觸及(ji)板(ban)(ban)(ban)壁(bi)）或(huo)(huo)甩掉(diao)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)內(nei)(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)；在(zai)(zai)實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)臺上(shang)(shang)鋪墊幾(ji)層吸(xi)(xi)水紙，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)朝下(xia)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)力(li)拍幾(ji)次(ci)；將(jiang)推薦(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)(xi)滌緩(huan)沖液(ye)(ye)(ye)(ye)(ye)(ye)至少0.3ml注入(ru)孔(kong)(kong)(kong)內(nei)(nei)(nei)(nei)，浸泡1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。根(gen)(gen)(gen)(gen)據(ju)需要(yao)，重(zhong)(zhong)復此過(guo)(guo)(guo)(guo)(guo)程(cheng)數(shu)(shu)(shu)次(ci)。 自動(dong)(dong)洗(xi)(xi)(xi)板(ban)(ban)(ban)：如(ru)(ru)果(guo)有(you)(you)(you)自動(dong)(dong)洗(xi)(xi)(xi)板(ban)(ban)(ban)機，應(ying)(ying)在(zai)(zai)熟練使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)再用(yong)(yong)(yong)(yong)(yong)(yong)(yong)到正(zheng)式實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)過(guo)(guo)(guo)(guo)(guo)程(cheng)中。 計(ji)算(suan) 以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)半對數(shu)(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)繪出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)，根(gen)(gen)(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)查(cha)出(chu)(chu)相(xiang)(xiang)(xiang)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)；再乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)(shu)；或(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)與(yu)OD值(zhi)計(ji)算(suan)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)(xian)回(hui)歸方(fang)(fang)程(cheng)式，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代入(ru)方(fang)(fang)程(cheng)式，計(ji)算(suan)出(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)，再乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)(shu)，即為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)(shi)(shi)(shi)際(ji)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)。 五、技(ji)術小(xiao)(xiao)(xiao)提示(shi)1.當(dang)混(hun)(hun)合蛋(dan)白(bai)(bai)(bai)(bai)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)盡(jin)量(liang)(liang)(liang)(liang)輕(qing)(qing)(qing)緩(huan)，避(bi)(bi)免(mian)起(qi)泡。2. 洗(xi)(xi)(xi)滌過(guo)(guo)(guo)(guo)(guo)程(cheng)非(fei)常重(zhong)(zhong)要(yao)，不(bu)(bu)充分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)(xi)滌易造成假陽性。 3. 一(yi)次(ci)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)間控制(zhi)(zhi)在(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)多(duo)，推薦(jian)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)排(pai)槍(qiang)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)。 4. 請(qing)(qing)每(mei)(mei)(mei)次(ci)測(ce)(ce)定的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi)(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)，做(zuo)復孔(kong)(kong)(kong)。 5. 如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)中待測(ce)(ce)物(wu)(wu)(wu)質含(han)量(liang)(liang)(liang)(liang)過(guo)(guo)(guo)(guo)(guo)高，請(qing)(qing)先(xian)(xian)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)再測(ce)(ce)定，計(ji)算(suan)時(shi)(shi)(shi)(shi)(shi)請(qing)(qing)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)(shu)。 6. 在(zai)(zai)配(pei)(pei)(pei)(pei)制(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)測(ce)(ce)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)，請(qing)(qing)以(yi)(yi)(yi)(yi)相(xiang)(xiang)(xiang)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)制(zhi)(zhi)，不(bu)(bu)能混(hun)(hun)淆。 7. 底(di)物(wu)(wu)(wu)請(qing)(qing)避(bi)(bi)光保(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)。 8. 不(bu)(bu)要(yao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)其(qi)它生(sheng)(sheng)產廠家(jia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)替換試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)中的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)。 六(liu)、注意事項1.收集(ji)(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)前(qian)必須清(qing)(qing)楚要(yao)檢(jian)(jian)(jian)測(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成份是否足(zu)夠穩定。對收集(ji)(ji)(ji)(ji)后(hou)(hou)(hou)當(dang)天(tian)進行(xing)檢(jian)(jian)(jian)測(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)，儲存(cun)(cun)(cun)(cun)(cun)(cun)在(zai)(zai)4 ℃備(bei)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)有(you)(you)(you)特殊(shu)原(yuan)因需要(yao)周期(qi)(qi)收集(ji)(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)， 將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)及(ji)時(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝后(hou)(hou)(hou)放(fang)在(zai)(zai)-20 ℃或(huo)(huo)-70 ℃條(tiao)(tiao)件(jian)(jian)下(xia)保(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)。避(bi)(bi)免(mian)反(fan)(fan)(fan)復凍(dong)(dong)融(rong)(rong)(rong)。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)2 -8 ℃可(ke)保(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)48 小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)保(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)1 個(ge)月(yue)。-70度(du)(du)(du)可(ke)保(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)6 個(ge)月(yue)。3.部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)激素(su)(su)(su)(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)需添(tian)加(jia)(jia)抑(yi)肽酶(mei)(mei)(mei)。 4.計(ji)算(suan)方(fang)(fang)法：以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)繪出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)，根(gen)(gen)(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)查(cha)出(chu)(chu)相(xiang)(xiang)(xiang)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)；再乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)(shu)；或(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)與(yu)OD值(zhi)計(ji)算(suan)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)(xian)回(hui)歸方(fang)(fang)程(cheng)式，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代入(ru)方(fang)(fang)程(cheng)式，計(ji)算(suan)出(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)，再乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)(shu)，即為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)(shi)(shi)(shi)際(ji)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)。 七、試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)性能1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)性回(hui)歸與(yu)預(yu)期(qi)(qi)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)相(xiang)(xiang)(xiang)關(guan)系(xi)數(shu)(shu)(shu)R值(zhi)為(wei)(wei)(wei)(wei)0.92以(yi)(yi)(yi)(yi)上(shang)(shang)。2.批(pi)內(nei)(nei)(nei)(nei)與(yu)批(pi)見應(ying)(ying)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)小(xiao)(xiao)(xiao)于(yu)(yu)9%和(he)15% 八、保(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)條(tiao)(tiao)件(jian)(jian)及(ji)有(you)(you)(you)效期(qi)(qi)1.試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)保(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)：2-8℃。 2．有(you)(you)(you)效期(qi)(qi)：6個(ge)月(yue)

 小(xiao)(xiao)鼠神(shen)經(jing)膠質纖維酸性(xing)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)（GFAP）實(shi)(shi)驗(yan)(yan)(yan)(yan)原(yuan)理   試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)采用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙(shuang)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)一(yi)步(bu)夾心(xin)法酶(mei)(mei)(mei)(mei)聯免(mian)(mian)疫吸(xi)附試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)（ELISA）。往預(yu)先(xian)包被脂(zhi)肪(fang)酸結(jie)(jie)合蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)1(FABP1)捕(bu)獲(huo)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)(kong)(kong)中(zhong)，依次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)，經(jing)過(guo)(guo)(guo)溫(wen)育(yu)并徹(che)底(di)(di)(di)(di)洗(xi)滌(di)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯(xian)(xian)色(se)，TMB在(zai)(zai)過(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催(cui)化(hua)(hua)(hua)下(xia)轉(zhuan)化(hua)(hua)(hua)成藍色(se)，并在(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)下(xia)轉(zhuan)化(hua)(hua)(hua)成＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)。顏(yan)色(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深(shen)淺和(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂(zhi)肪(fang)酸結(jie)(jie)合蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)1(FABP1)呈正相(xiang)關。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)在(zai)(zai)450nm 波長下(xia)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)吸(xi)光度(du)(du)(du)(du)(du)（OD 值(zhi)(zhi)(zhi)），計(ji)(ji)(ji)算(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)。保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)條(tiao)件及(ji)(ji)(ji)有(you)(you)效(xiao)期(qi)本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)外(wai)定(ding)量(liang)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)血(xue)清、血(xue)漿(jiang)、組織勻(yun)漿(jiang)及(ji)(ji)(ji)相(xiang)關液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)中(zhong)脂(zhi)肪(fang)酸結(jie)(jie)合蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)。有(you)(you)效(xiao)期(qi)：6個月(yue)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)條(tiao)件：2-8℃小(xiao)(xiao)鼠神(shen)經(jing)膠質纖維酸性(xing)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)（GFAP）一(yi)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采集(ji)及(ji)(ji)(ji)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun) 1.血(xue)清：全血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)請(qing)(qing)(qing)于(yu)(yu)(yu)(yu)(yu)室溫(wen)放(fang)(fang)置(zhi)2小(xiao)(xiao)時(shi)(shi)(shi)(shi)或(huo)(huo)(huo)4℃過(guo)(guo)(guo)一(yi)晚后(hou)(hou)于(yu)(yu)(yu)(yu)(yu)1000 x g離(li)(li)心(xin)20分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，取(qu)上(shang)(shang)(shang)清即可(ke)(ke)(ke)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放(fang)(fang)于(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)，但應(ying)(ying)避(bi)免(mian)(mian)反(fan)(fan)復(fu)(fu)(fu)凍(dong)融。 2.血(xue)漿(jiang)：可(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)肝素(su)(su)(su)作(zuo)(zuo)為(wei)(wei)(wei)抗(kang)(kang)(kang)(kang)(kang)凝劑(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)采集(ji)后(hou)(hou)30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)于(yu)(yu)(yu)(yu)(yu)2 - 8° C 1000 x g離(li)(li)心(xin)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放(fang)(fang)于(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)，但應(ying)(ying)避(bi)免(mian)(mian)反(fan)(fan)復(fu)(fu)(fu)凍(dong)融。 3.細胞培(pei)養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)清或(huo)(huo)(huo)其它(ta)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)：1000 x g離(li)(li)心(xin)20分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，取(qu)上(shang)(shang)(shang)清即可(ke)(ke)(ke)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放(fang)(fang)于(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)，但應(ying)(ying)避(bi)免(mian)(mian)反(fan)(fan)復(fu)(fu)(fu)凍(dong)融。 注：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)溶(rong)(rong)血(xue)會影響(xiang)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)果，因(yin)此(ci)(ci)溶(rong)(rong)血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)不(bu)(bu)宜(yi)進行(xing)此(ci)(ci)項(xiang)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)。二、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)   首(shou)先(xian)通過(guo)(guo)(guo)文獻檢(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)式(shi)了(le)解待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含量(liang)，確定(ding)適當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)。只有(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)至(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍(wei)內(nei)(nei)(nei)(nei)，檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)(jie)果才是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)程(cheng)中(zhong)，應(ying)(ying)做好(hao)詳細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)錄。計(ji)(ji)(ji)算(suan)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)時(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)了(le)“N”倍，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)應(ying)(ying)再(zai)(zai)乘(cheng)(cheng)(cheng)以(yi)(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：2瓶，每(mei)(mei)(mei)(mei)瓶臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)至(zhi)1ml，蓋(gai)好(hao)后(hou)(hou)靜置(zhi)10分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)上(shang)(shang)(shang)，然(ran)后(hou)(hou)反(fan)(fan)復(fu)(fu)(fu)顛倒/搓(cuo)動(dong)以(yi)(yi)助溶(rong)(rong)解，其濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)為(wei)(wei)(wei)300 pg/ml，做系列倍比(bi)(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)，分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)直接作(zuo)(zuo)為(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)。 如(ru)配(pei)(pei)(pei)(pei)制(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)：取(qu)0.5ml（不(bu)(bu)要(yao)少(shao)于(yu)(yu)(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)含0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)中(zhong)，混(hun)勻(yun)即可(ke)(ke)(ke)，其余濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)以(yi)(yi)此(ci)(ci)類推(tui)(tui)。2.生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)： 臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)前(qian)(qian)根(gen)(gen)(gen)據(ju)預(yu)先(xian)計(ji)(ji)(ji)算(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)實(shi)(shi)驗(yan)(yan)(yan)(yan)所(suo)(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)100μl），實(shi)(shi)際(ji)(ji)配(pei)(pei)(pei)(pei)制(zhi)(zhi)時(shi)(shi)(shi)(shi)應(ying)(ying)多配(pei)(pei)(pei)(pei)制(zhi)(zhi)0.1-0.2ml。如(ru)10μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)例配(pei)(pei)(pei)(pei)制(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)勻(yun)，在(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)。3.辣(la)(la)根(gen)(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)和(he)(he)素(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)： 臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)辣(la)(la)根(gen)(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)和(he)(he)素(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)前(qian)(qian)根(gen)(gen)(gen)據(ju)預(yu)先(xian)計(ji)(ji)(ji)算(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)實(shi)(shi)驗(yan)(yan)(yan)(yan)所(suo)(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)100μl），實(shi)(shi)際(ji)(ji)配(pei)(pei)(pei)(pei)制(zhi)(zhi)時(shi)(shi)(shi)(shi)應(ying)(ying)多配(pei)(pei)(pei)(pei)制(zhi)(zhi)0.1-0.2ml。如(ru)10μl辣(la)(la)根(gen)(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)和(he)(he)素(su)(su)(su)加(jia)(jia)(jia)(jia)(jia)990μl辣(la)(la)根(gen)(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)和(he)(he)素(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)例配(pei)(pei)(pei)(pei)制(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)勻(yun)，在(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)。 操(cao)作(zuo)(zuo)步(bu)驟 實(shi)(shi)驗(yan)(yan)(yan)(yan)開始前(qian)(qian)，請(qing)(qing)(qing)提(ti)前(qian)(qian)配(pei)(pei)(pei)(pei)置(zhi)好(hao)所(suo)(suo)有(you)(you)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)或(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)，均(jun)需(xu)混(hun)勻(yun)，混(hun)勻(yun)時(shi)(shi)(shi)(shi)盡量(liang)避(bi)免(mian)(mian)起泡。每(mei)(mei)(mei)(mei)次(ci)(ci)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)該做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)。如(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)過(guo)(guo)(guo)高時(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)進行(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)使(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)符合試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)范圍(wei)。 小(xiao)(xiao)鼠神(shen)經(jing)膠質纖維酸性(xing)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)（GFAP）三、操(cao)作(zuo)(zuo)步(bu)驟1.加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)設(she)空白(bai)(bai)(bai)孔(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)、待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)。空白(bai)(bai)(bai)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)100μl，注意不(bu)(bu)要(yao)有(you)(you)氣泡，加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)于(yu)(yu)(yu)(yu)(yu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)底(di)(di)(di)(di)部(bu)，盡量(liang)不(bu)(bu)觸及(ji)(ji)(ji)孔(kong)(kong)(kong)壁(bi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)晃動(dong)混(hun)勻(yun)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)蓋(gai)或(huo)(huo)(huo)覆膜，37℃反(fan)(fan)應(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)保(bao)(bao)(bao)(bao)(bao)(bao)證實(shi)(shi)驗(yan)(yan)(yan)(yan)結(jie)(jie)果有(you)(you)效(xiao)性(xing)，每(mei)(mei)(mei)(mei)次(ci)(ci)實(shi)(shi)驗(yan)(yan)(yan)(yan)請(qing)(qing)(qing)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)新(xin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)。2.棄(qi)去液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)，甩(shuai)干(gan)，不(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)滌(di)。每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取(qu)1μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)例配(pei)(pei)(pei)(pei)制(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)勻(yun)，在(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。 3.溫(wen)育(yu)60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)，棄(qi)去孔(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)，甩(shuai)干(gan)，洗(xi)板(ban)(ban)(ban)(ban)(ban)3次(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)浸(jin)(jin)泡1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)，甩(shuai)干(gan)。 4.每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)辣(la)(la)根(gen)(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)和(he)(he)素(su)(su)(su)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)（同生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。 5.溫(wen)育(yu)60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)，棄(qi)去孔(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)，甩(shuai)干(gan)，洗(xi)板(ban)(ban)(ban)(ban)(ban)5次(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)浸(jin)(jin)泡1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)，甩(shuai)干(gan)。 6.依序(xu)(xu)每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)光顯(xian)(xian)色(se)（30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)，此(ci)(ci)時(shi)(shi)(shi)(shi)肉眼可(ke)(ke)(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)3-4孔(kong)(kong)(kong)有(you)(you)明(ming)顯(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)(du)(du)藍色(se)，后(hou)(hou)3-4孔(kong)(kong)(kong)梯度(du)(du)(du)(du)(du)不(bu)(bu)明(ming)顯(xian)(xian)，即可(ke)(ke)(ke)終(zhong)止(zhi)(zhi)(zhi)）。 7.依序(xu)(xu)每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)(zhi)(zhi)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止(zhi)(zhi)(zhi)反(fan)(fan)應(ying)(ying)（此(ci)(ci)時(shi)(shi)(shi)(shi)藍色(se)立轉(zhuan)黃色(se)）。終(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)順(shun)序(xu)(xu)應(ying)(ying)盡量(liang)與底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)順(shun)序(xu)(xu)相(xiang)同。為(wei)(wei)(wei)了(le)保(bao)(bao)(bao)(bao)(bao)(bao)證實(shi)(shi)驗(yan)(yan)(yan)(yan)結(jie)(jie)果的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)，底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)(fan)應(ying)(ying)時(shi)(shi)(shi)(shi)間到(dao)(dao)(dao)后(hou)(hou)應(ying)(ying)盡快加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)終(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)。 8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)聯儀(yi)在(zai)(zai)450nm波長依序(xu)(xu)測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)各(ge)孔(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光密度(du)(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)）。 在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)內(nei)(nei)(nei)(nei)進行(xing)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)。 四、試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)小(xiao)(xiao)技巧1. 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)初次(ci)(ci)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)時(shi)(shi)(shi)(shi)，應(ying)(ying)將(jiang)(jiang)(jiang)(jiang)各(ge)種(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)管(guan)離(li)(li)心(xin)數(shu)(shu)(shu)(shu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，以(yi)(yi)便試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)集(ji)中(zhong)到(dao)(dao)(dao)管(guan)底(di)(di)(di)(di)。2. 每(mei)(mei)(mei)(mei)次(ci)(ci)實(shi)(shi)驗(yan)(yan)(yan)(yan)留一(yi)孔(kong)(kong)(kong)作(zuo)(zuo)為(wei)(wei)(wei)空白(bai)(bai)(bai)調(diao)零孔(kong)(kong)(kong)，該孔(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)(jia)任(ren)何試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，只是**加(jia)(jia)(jia)(jia)(jia)底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)及(ji)(ji)(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)時(shi)(shi)(shi)(shi)先(xian)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)此(ci)(ci)孔(kong)(kong)(kong)調(diao)OD值(zhi)(zhi)(zhi)至(zhi)零。 3. 為(wei)(wei)(wei)防止(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)時(shi)(shi)(shi)(shi)將(jiang)(jiang)(jiang)(jiang)反(fan)(fan)應(ying)(ying)板(ban)(ban)(ban)(ban)(ban)放(fang)(fang)于(yu)(yu)(yu)(yu)(yu)鋪(pu)(pu)有(you)(you)濕(shi)布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)(he)內(nei)(nei)(nei)(nei)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)蓋(gai)或(huo)(huo)(huo)覆膜。 4. 未使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)或(huo)(huo)(huo)者試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，請(qing)(qing)(qing)于(yu)(yu)(yu)(yu)(yu)2-8℃保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)(la)根(gen)(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)和(he)(he)素(su)(su)(su)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)請(qing)(qing)(qing)依據(ju)所(suo)(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)配(pei)(pei)(pei)(pei)置(zhi)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)(qing)勿(wu)重復(fu)(fu)(fu)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)、辣(la)(la)根(gen)(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)和(he)(he)素(su)(su)(su)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)。 5. 建(jian)議檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)均(jun)設(she)雙(shuang)孔(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)，以(yi)(yi)保(bao)(bao)(bao)(bao)(bao)(bao)證檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)果的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)。 洗(xi)板(ban)(ban)(ban)(ban)(ban)方(fang)(fang)法 手(shou)工(gong)(gong)洗(xi)板(ban)(ban)(ban)(ban)(ban)方(fang)(fang)法：吸(xi)去（不(bu)(bu)可(ke)(ke)(ke)觸及(ji)(ji)(ji)板(ban)(ban)(ban)(ban)(ban)壁(bi)）或(huo)(huo)(huo)甩(shuai)掉酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)內(nei)(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)；在(zai)(zai)實(shi)(shi)驗(yan)(yan)(yan)(yan)臺上(shang)(shang)(shang)鋪(pu)(pu)墊幾(ji)層吸(xi)水紙，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)朝下(xia)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)力拍幾(ji)次(ci)(ci)；將(jiang)(jiang)(jiang)(jiang)推(tui)(tui)薦(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌(di)緩沖液(ye)(ye)(ye)(ye)(ye)(ye)至(zhi)少(shao)0.3ml注入(ru)(ru)孔(kong)(kong)(kong)內(nei)(nei)(nei)(nei)，浸(jin)(jin)泡1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。根(gen)(gen)(gen)據(ju)需(xu)要(yao)，重復(fu)(fu)(fu)此(ci)(ci)過(guo)(guo)(guo)程(cheng)數(shu)(shu)(shu)(shu)次(ci)(ci)。 自動(dong)洗(xi)板(ban)(ban)(ban)(ban)(ban)：如(ru)果有(you)(you)自動(dong)洗(xi)板(ban)(ban)(ban)(ban)(ban)機，應(ying)(ying)在(zai)(zai)熟練使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)再(zai)(zai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)到(dao)(dao)(dao)正式(shi)實(shi)(shi)驗(yan)(yan)(yan)(yan)過(guo)(guo)(guo)程(cheng)中(zhong)。 計(ji)(ji)(ji)算(suan) 以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)為(wei)(wei)(wei)橫(heng)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)(shu)(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)半對數(shu)(shu)(shu)(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)，根(gen)(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)查出(chu)相(xiang)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)；再(zai)(zai)乘(cheng)(cheng)(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)與OD值(zhi)(zhi)(zhi)計(ji)(ji)(ji)算(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)(xian)回(hui)歸(gui)方(fang)(fang)程(cheng)式(shi)，將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代(dai)入(ru)(ru)方(fang)(fang)程(cheng)式(shi)，計(ji)(ji)(ji)算(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)，再(zai)(zai)乘(cheng)(cheng)(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)，即為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際(ji)(ji)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)。 五、技術小(xiao)(xiao)提(ti)示(shi)1.當(dang)混(hun)合蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)應(ying)(ying)盡量(liang)輕(qing)(qing)(qing)緩，避(bi)免(mian)(mian)起泡。2. 洗(xi)滌(di)過(guo)(guo)(guo)程(cheng)非常重要(yao)，不(bu)(bu)充分(fen)(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌(di)易造成假陽(yang)性(xing)。 3. 一(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)間控制(zhi)(zhi)在(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)數(shu)(shu)(shu)(shu)量(liang)多，推(tui)(tui)薦(jian)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。 4. 請(qing)(qing)(qing)每(mei)(mei)(mei)(mei)次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)，做復(fu)(fu)(fu)孔(kong)(kong)(kong)。 5. 如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)中(zhong)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)過(guo)(guo)(guo)高，請(qing)(qing)(qing)先(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)再(zai)(zai)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)，計(ji)(ji)(ji)算(suan)時(shi)(shi)(shi)(shi)請(qing)(qing)(qing)乘(cheng)(cheng)(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)。 6. 在(zai)(zai)配(pei)(pei)(pei)(pei)制(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)，請(qing)(qing)(qing)以(yi)(yi)相(xiang)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)制(zhi)(zhi)，不(bu)(bu)能混(hun)淆。 7. 底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)(qing)避(bi)光保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)。 8. 不(bu)(bu)要(yao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)其它(ta)生(sheng)(sheng)(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)替換試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)。 六、注意事項(xiang)1.收集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)前(qian)(qian)必須(xu)清楚要(yao)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成份是否(fou)足夠穩定(ding)。對收集(ji)后(hou)(hou)當(dang)天進行(xing)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)，儲存(cun)(cun)在(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)有(you)(you)特殊原(yuan)因(yin)需(xu)要(yao)周期(qi)收集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)， 將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)及(ji)(ji)(ji)時(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝后(hou)(hou)放(fang)(fang)在(zai)(zai)-20 ℃或(huo)(huo)(huo)-70 ℃條(tiao)件下(xia)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)。避(bi)免(mian)(mian)反(fan)(fan)復(fu)(fu)(fu)凍(dong)融。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)2 -8 ℃可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)48 小(xiao)(xiao)時(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)1 個月(yue)。-70度(du)(du)(du)(du)(du)可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)6 個月(yue)。3.部(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)激素(su)(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)需(xu)添加(jia)(jia)(jia)(jia)(jia)抑肽酶(mei)(mei)(mei)(mei)。 4.計(ji)(ji)(ji)算(suan)方(fang)(fang)法：以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)為(wei)(wei)(wei)橫(heng)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)，根(gen)(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)查出(chu)相(xiang)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)；再(zai)(zai)乘(cheng)(cheng)(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)與OD值(zhi)(zhi)(zhi)計(ji)(ji)(ji)算(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)(xian)回(hui)歸(gui)方(fang)(fang)程(cheng)式(shi)，將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代(dai)入(ru)(ru)方(fang)(fang)程(cheng)式(shi)，計(ji)(ji)(ji)算(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)，再(zai)(zai)乘(cheng)(cheng)(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)，即為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際(ji)(ji)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)。 七、試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)性(xing)能1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)線(xian)(xian)(xian)(xian)性(xing)回(hui)歸(gui)與預(yu)期(qi)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)相(xiang)關系數(shu)(shu)(shu)(shu)R值(zhi)(zhi)(zhi)為(wei)(wei)(wei)0.92以(yi)(yi)上(shang)(shang)(shang)。2.批內(nei)(nei)(nei)(nei)與批見應(ying)(ying)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)小(xiao)(xiao)于(yu)(yu)(yu)(yu)(yu)9%和(he)(he)15% 八、保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)條(tiao)件及(ji)(ji)(ji)有(you)(you)效(xiao)期(qi)1.試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)：2-8℃。 2．有(you)(you)效(xiao)期(qi)：6個月(yue)

 小(xiao)(xiao)(xiao)鼠(shu)總(zong) Tau 蛋(dan)(dan)(dan)白（T-Tau）ELISA 檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)實(shi)驗(yan)(yan)原(yuan)(yuan)理   試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)采用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙(shuang)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)一(yi)(yi)(yi)步(bu)夾心法(fa)酶(mei)(mei)(mei)(mei)聯(lian)免疫吸附試(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)（ELISA）。往預(yu)(yu)先包被(bei)脂(zhi)肪(fang)酸結(jie)合(he)蛋(dan)(dan)(dan)白1(FABP1)捕(bu)獲抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被(bei)微孔(kong)(kong)(kong)(kong)中(zhong)，依(yi)次(ci)加(jia)(jia)(jia)(jia)(jia)入(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)，經過(guo)(guo)(guo)溫育并(bing)徹底(di)(di)洗(xi)(xi)(xi)滌(di)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)底(di)(di)物(wu)(wu)(wu)(wu)(wu)TMB顯(xian)(xian)色(se)(se)，TMB在(zai)(zai)(zai)過(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)(hua)(hua)(hua)下轉化(hua)(hua)(hua)(hua)(hua)成(cheng)藍(lan)色(se)(se)，并(bing)在(zai)(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)下轉化(hua)(hua)(hua)(hua)(hua)成(cheng)＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)(se)。顏色(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂(zhi)肪(fang)酸結(jie)合(he)蛋(dan)(dan)(dan)白1(FABP1)呈正(zheng)相(xiang)(xiang)關(guan)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)(zai)450nm 波長下測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)吸光度(du)(du)(du)(du)(du)（OD 值(zhi)），計算(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)。保(bao)(bao)存條(tiao)(tiao)件及(ji)(ji)(ji)有(you)(you)(you)(you)(you)效(xiao)期本(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)體(ti)(ti)(ti)外定(ding)(ding)(ding)量(liang)(liang)(liang)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)血(xue)(xue)清(qing)、血(xue)(xue)漿(jiang)、組織勻(yun)(yun)(yun)(yun)漿(jiang)及(ji)(ji)(ji)相(xiang)(xiang)關(guan)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)脂(zhi)肪(fang)酸結(jie)合(he)蛋(dan)(dan)(dan)白1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)(han)量(liang)(liang)(liang)。有(you)(you)(you)(you)(you)效(xiao)期：6個(ge)月保(bao)(bao)存條(tiao)(tiao)件：2-8℃小(xiao)(xiao)(xiao)鼠(shu)總(zong) Tau 蛋(dan)(dan)(dan)白（T-Tau）ELISA 檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)一(yi)(yi)(yi)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采集(ji)(ji)及(ji)(ji)(ji)保(bao)(bao)存 1.血(xue)(xue)清(qing)：全血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)請(qing)于(yu)(yu)(yu)室(shi)溫放置(zhi)2小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)(huo)(huo)4℃過(guo)(guo)(guo)一(yi)(yi)(yi)晚后(hou)于(yu)(yu)(yu)1000 x g離(li)(li)心20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，取(qu)上(shang)清(qing)即(ji)(ji)可(ke)(ke)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)放于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)存，但應(ying)(ying)(ying)(ying)(ying)避免反(fan)(fan)復(fu)凍(dong)融。 2.血(xue)(xue)漿(jiang)：可(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)(huo)肝素(su)(su)(su)作(zuo)(zuo)為(wei)(wei)(wei)抗(kang)(kang)(kang)(kang)凝劑(ji)(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)采集(ji)(ji)后(hou)30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)于(yu)(yu)(yu)2 - 8° C 1000 x g離(li)(li)心15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，或(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)放于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)存，但應(ying)(ying)(ying)(ying)(ying)避免反(fan)(fan)復(fu)凍(dong)融。 3.細(xi)胞培養物(wu)(wu)(wu)(wu)(wu)上(shang)清(qing)或(huo)(huo)(huo)(huo)(huo)其(qi)它(ta)生(sheng)物(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)：1000 x g離(li)(li)心20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，取(qu)上(shang)清(qing)即(ji)(ji)可(ke)(ke)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)放于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)存，但應(ying)(ying)(ying)(ying)(ying)避免反(fan)(fan)復(fu)凍(dong)融。 注：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)溶(rong)(rong)血(xue)(xue)會(hui)影響檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)，因此(ci)溶(rong)(rong)血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)不(bu)(bu)宜進行此(ci)項檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。二、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)則(ze)(ze)   首先通過(guo)(guo)(guo)文(wen)獻檢(jian)(jian)(jian)(jian)索(suo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)式(shi)(shi)(shi)(shi)了解(jie)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含(han)(han)量(liang)(liang)(liang)，確(que)定(ding)(ding)(ding)適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)。只(zhi)有(you)(you)(you)(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范(fan)圍內(nei)(nei)(nei)(nei)(nei)，檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果(guo)才是(shi)(shi)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)程(cheng)(cheng)中(zhong)，應(ying)(ying)(ying)(ying)(ying)做好(hao)詳細(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)錄。計算(suan)(suan)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)應(ying)(ying)(ying)(ying)(ying)再乘以(yi)(yi)(yi)(yi)(yi)(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)則(ze)(ze)：2瓶，每(mei)(mei)(mei)(mei)(mei)(mei)(mei)瓶臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至1ml，蓋(gai)好(hao)后(hou)靜(jing)置(zhi)10分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)(yi)上(shang)，然后(hou)反(fan)(fan)復(fu)顛(dian)倒/搓(cuo)動(dong)以(yi)(yi)(yi)(yi)(yi)(yi)助溶(rong)(rong)解(jie)，其(qi)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)為(wei)(wei)(wei)300 pg/ml，做系列倍(bei)比(bi)(bi)(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)，分(fen)(fen)(fen)(fen)別(bie)(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)直接(jie)作(zuo)(zuo)為(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)0 pg/ml，臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)(qian)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)。 如(ru)(ru)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取(qu)0.5ml（不(bu)(bu)要(yao)(yao)(yao)少于(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)述(shu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)入(ru)含(han)(han)0.5ml樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中(zhong)，混(hun)勻(yun)(yun)(yun)(yun)即(ji)(ji)可(ke)(ke)，其(qi)余濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)以(yi)(yi)(yi)(yi)(yi)(yi)此(ci)類(lei)推。2.生(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)則(ze)(ze)： 臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)生(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)(qian)根據預(yu)(yu)先計算(suan)(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)(mei)(mei)(mei)次(ci)實(shi)驗(yan)(yan)所(suo)需(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)100μl），實(shi)際配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)多(duo)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl生(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)990μl生(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)勻(yun)(yun)(yun)(yun)，在(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)(qian)一(yi)(yi)(yi)小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)。3.辣(la)(la)根過(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和素(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)則(ze)(ze)： 臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)辣(la)(la)根過(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和素(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)(qian)根據預(yu)(yu)先計算(suan)(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)(mei)(mei)(mei)次(ci)實(shi)驗(yan)(yan)所(suo)需(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)100μl），實(shi)際配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)多(duo)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl辣(la)(la)根過(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和素(su)(su)(su)加(jia)(jia)(jia)(jia)(jia)990μl辣(la)(la)根過(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和素(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)勻(yun)(yun)(yun)(yun)，在(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)(qian)一(yi)(yi)(yi)小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)。 操作(zuo)(zuo)步(bu)驟 實(shi)驗(yan)(yan)開始前(qian)(qian)(qian)(qian)(qian)，請(qing)提(ti)前(qian)(qian)(qian)(qian)(qian)配(pei)(pei)(pei)(pei)置(zhi)好(hao)所(suo)有(you)(you)(you)(you)(you)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)或(huo)(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)，均需(xu)(xu)(xu)混(hun)勻(yun)(yun)(yun)(yun)，混(hun)勻(yun)(yun)(yun)(yun)時(shi)(shi)(shi)(shi)(shi)(shi)盡(jin)(jin)量(liang)(liang)(liang)避免起泡(pao)。每(mei)(mei)(mei)(mei)(mei)(mei)(mei)次(ci)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)(ying)該(gai)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)(xian)。如(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)過(guo)(guo)(guo)高時(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)進行稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)(yi)(yi)使(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)符(fu)合(he)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)圍。 小(xiao)(xiao)(xiao)鼠(shu)總(zong) Tau 蛋(dan)(dan)(dan)白（T-Tau）ELISA 檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)三、操作(zuo)(zuo)步(bu)驟1.加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)別(bie)(bie)設空白孔(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)、待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)。空白孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)(huo)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注意不(bu)(bu)要(yao)(yao)(yao)有(you)(you)(you)(you)(you)氣泡(pao)，加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)于(yu)(yu)(yu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)孔(kong)(kong)(kong)(kong)底(di)(di)部，盡(jin)(jin)量(liang)(liang)(liang)不(bu)(bu)觸及(ji)(ji)(ji)孔(kong)(kong)(kong)(kong)壁，輕(qing)(qing)(qing)輕(qing)(qing)(qing)晃(huang)動(dong)混(hun)勻(yun)(yun)(yun)(yun)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)加(jia)(jia)(jia)(jia)(jia)上(shang)蓋(gai)或(huo)(huo)(huo)(huo)(huo)覆膜(mo)，37℃反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)保(bao)(bao)證(zheng)實(shi)驗(yan)(yan)結(jie)果(guo)有(you)(you)(you)(you)(you)效(xiao)性(xing)(xing)(xing)，每(mei)(mei)(mei)(mei)(mei)(mei)(mei)次(ci)實(shi)驗(yan)(yan)請(qing)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)新(xin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)。2.棄去液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干，不(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)(xi)滌(di)。每(mei)(mei)(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)生(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye) 100μl（取(qu)1μl生(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)99μl生(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)勻(yun)(yun)(yun)(yun)，在(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)(qian)一(yi)(yi)(yi)小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 3.溫育60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)，棄去孔(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干，洗(xi)(xi)(xi)板(ban)3次(ci)，每(mei)(mei)(mei)(mei)(mei)(mei)(mei)次(ci)浸(jin)泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)，甩(shuai)干。 4.每(mei)(mei)(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)辣(la)(la)根過(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和素(su)(su)(su)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)（同生(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 5.溫育60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)，棄去孔(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干，洗(xi)(xi)(xi)板(ban)5次(ci)，每(mei)(mei)(mei)(mei)(mei)(mei)(mei)次(ci)浸(jin)泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)，甩(shuai)干。 6.依(yi)序(xu)(xu)每(mei)(mei)(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)90μl，37℃避光顯(xian)(xian)色(se)(se)（30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)，此(ci)時(shi)(shi)(shi)(shi)(shi)(shi)肉眼可(ke)(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)(qian)(qian)3-4孔(kong)(kong)(kong)(kong)有(you)(you)(you)(you)(you)明顯(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)(du)(du)藍(lan)色(se)(se)，后(hou)3-4孔(kong)(kong)(kong)(kong)梯度(du)(du)(du)(du)(du)不(bu)(bu)明顯(xian)(xian)，即(ji)(ji)可(ke)(ke)終(zhong)止(zhi)(zhi)）。 7.依(yi)序(xu)(xu)每(mei)(mei)(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)(zhi)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止(zhi)(zhi)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)（此(ci)時(shi)(shi)(shi)(shi)(shi)(shi)藍(lan)色(se)(se)立轉黃色(se)(se)）。終(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)入(ru)順序(xu)(xu)應(ying)(ying)(ying)(ying)(ying)盡(jin)(jin)量(liang)(liang)(liang)與底(di)(di)物(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)入(ru)順序(xu)(xu)相(xiang)(xiang)同。為(wei)(wei)(wei)了保(bao)(bao)證(zheng)實(shi)驗(yan)(yan)結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)(xing)，底(di)(di)物(wu)(wu)(wu)(wu)(wu)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)間(jian)到后(hou)應(ying)(ying)(ying)(ying)(ying)盡(jin)(jin)快加(jia)(jia)(jia)(jia)(jia)入(ru)終(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)。 8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)聯(lian)儀在(zai)(zai)(zai)450nm波長依(yi)序(xu)(xu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)各(ge)孔(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光密度(du)(du)(du)(du)(du)（OD值(zhi)）。 在(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)后(hou)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)(yi)內(nei)(nei)(nei)(nei)(nei)進行檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。 四、試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)小(xiao)(xiao)(xiao)技(ji)巧1. 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)(zai)初次(ci)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)時(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)(ying)將(jiang)(jiang)各(ge)種試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)管離(li)(li)心數(shu)(shu)(shu)分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)集(ji)(ji)中(zhong)到管底(di)(di)。2. 每(mei)(mei)(mei)(mei)(mei)(mei)(mei)次(ci)實(shi)驗(yan)(yan)留(liu)一(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)作(zuo)(zuo)為(wei)(wei)(wei)空白調(diao)零孔(kong)(kong)(kong)(kong)，該(gai)孔(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)(jia)任(ren)何(he)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，只(zhi)是(shi)(shi)**加(jia)(jia)(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)及(ji)(ji)(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)時(shi)(shi)(shi)(shi)(shi)(shi)先用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)此(ci)孔(kong)(kong)(kong)(kong)調(diao)OD值(zhi)至零。 3. 為(wei)(wei)(wei)防止(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)時(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)板(ban)放于(yu)(yu)(yu)鋪有(you)(you)(you)(you)(you)濕(shi)布(bu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉(bi)盒(he)(he)內(nei)(nei)(nei)(nei)(nei)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)加(jia)(jia)(jia)(jia)(jia)上(shang)蓋(gai)或(huo)(huo)(huo)(huo)(huo)覆膜(mo)。 4. 未使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)或(huo)(huo)(huo)(huo)(huo)者試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，請(qing)于(yu)(yu)(yu)2-8℃保(bao)(bao)存。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)、辣(la)(la)根過(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和素(su)(su)(su)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)請(qing)依(yi)據所(suo)需(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)置(zhi)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)勿重(zhong)(zhong)復(fu)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)(huo)、辣(la)(la)根過(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和素(su)(su)(su)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)。 5. 建議檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)均設雙(shuang)孔(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，以(yi)(yi)(yi)(yi)(yi)(yi)保(bao)(bao)證(zheng)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)(xing)。 洗(xi)(xi)(xi)板(ban)方(fang)(fang)法(fa) 手工(gong)洗(xi)(xi)(xi)板(ban)方(fang)(fang)法(fa)：吸去（不(bu)(bu)可(ke)(ke)觸及(ji)(ji)(ji)板(ban)壁）或(huo)(huo)(huo)(huo)(huo)甩(shuai)掉(diao)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)內(nei)(nei)(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)；在(zai)(zai)(zai)實(shi)驗(yan)(yan)臺上(shang)鋪墊(dian)幾層吸水紙(zhi)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)朝下用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)力拍(pai)幾次(ci)；將(jiang)(jiang)推薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)(xi)滌(di)緩沖液(ye)(ye)(ye)(ye)(ye)至少0.3ml注入(ru)孔(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)，浸(jin)泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。根據需(xu)(xu)(xu)要(yao)(yao)(yao)，重(zhong)(zhong)復(fu)此(ci)過(guo)(guo)(guo)程(cheng)(cheng)數(shu)(shu)(shu)次(ci)。 自動(dong)洗(xi)(xi)(xi)板(ban)：如(ru)(ru)果(guo)有(you)(you)(you)(you)(you)自動(dong)洗(xi)(xi)(xi)板(ban)機，應(ying)(ying)(ying)(ying)(ying)在(zai)(zai)(zai)熟(shu)練使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)再用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)到正(zheng)式(shi)(shi)(shi)(shi)實(shi)驗(yan)(yan)過(guo)(guo)(guo)程(cheng)(cheng)中(zhong)。 計算(suan)(suan) 以(yi)(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)為(wei)(wei)(wei)橫(heng)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)為(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)半(ban)對數(shu)(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)(xian)，根據樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)(xian)查出(chu)相(xiang)(xiang)應(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)；再乘以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)；或(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)與OD值(zhi)計算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)回(hui)(hui)歸(gui)方(fang)(fang)程(cheng)(cheng)式(shi)(shi)(shi)(shi)，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代(dai)入(ru)方(fang)(fang)程(cheng)(cheng)式(shi)(shi)(shi)(shi)，計算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)，再乘以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)，即(ji)(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)。 五、技(ji)術小(xiao)(xiao)(xiao)提(ti)示1.當混(hun)合(he)蛋(dan)(dan)(dan)白溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)盡(jin)(jin)量(liang)(liang)(liang)輕(qing)(qing)(qing)緩，避免起泡(pao)。2. 洗(xi)(xi)(xi)滌(di)過(guo)(guo)(guo)程(cheng)(cheng)非常重(zhong)(zhong)要(yao)(yao)(yao)，不(bu)(bu)充分(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)(xi)滌(di)易造成(cheng)假陽性(xing)(xing)(xing)。 3. 一(yi)(yi)(yi)次(ci)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)間(jian)控制(zhi)(zhi)(zhi)(zhi)在(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)數(shu)(shu)(shu)量(liang)(liang)(liang)多(duo)，推薦使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)排(pai)槍加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)。 4. 請(qing)每(mei)(mei)(mei)(mei)(mei)(mei)(mei)次(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)(shi)(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)(xian)，做復(fu)孔(kong)(kong)(kong)(kong)。 5. 如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)質含(han)(han)量(liang)(liang)(liang)過(guo)(guo)(guo)高，請(qing)先稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)再測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，計算(suan)(suan)時(shi)(shi)(shi)(shi)(shi)(shi)請(qing)乘以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)。 6. 在(zai)(zai)(zai)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)以(yi)(yi)(yi)(yi)(yi)(yi)相(xiang)(xiang)應(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)，不(bu)(bu)能混(hun)淆。 7. 底(di)(di)物(wu)(wu)(wu)(wu)(wu)請(qing)避光保(bao)(bao)存。 8. 不(bu)(bu)要(yao)(yao)(yao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)其(qi)它(ta)生(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)替換試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)。 六、注意事項1.收集(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)前(qian)(qian)(qian)(qian)(qian)必須清(qing)楚要(yao)(yao)(yao)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)份是(shi)(shi)否足夠穩定(ding)(ding)(ding)。對收集(ji)(ji)后(hou)當天進行檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)，儲存在(zai)(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)有(you)(you)(you)(you)(you)特殊原(yuan)(yuan)因需(xu)(xu)(xu)要(yao)(yao)(yao)周(zhou)期收集(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)， 將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)及(ji)(ji)(ji)時(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)裝后(hou)放在(zai)(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)(huo)-70 ℃條(tiao)(tiao)件下保(bao)(bao)存。避免反(fan)(fan)復(fu)凍(dong)融。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)保(bao)(bao)存48 小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)保(bao)(bao)存1 個(ge)月。-70度(du)(du)(du)(du)(du)可(ke)(ke)保(bao)(bao)存6 個(ge)月。3.部分(fen)(fen)(fen)(fen)激(ji)素(su)(su)(su)類(lei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)添(tian)加(jia)(jia)(jia)(jia)(jia)抑肽酶(mei)(mei)(mei)(mei)。 4.計算(suan)(suan)方(fang)(fang)法(fa)：以(yi)(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)為(wei)(wei)(wei)橫(heng)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)為(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)(xian)，根據樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)(xian)查出(chu)相(xiang)(xiang)應(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)；再乘以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)；或(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)與OD值(zhi)計算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)回(hui)(hui)歸(gui)方(fang)(fang)程(cheng)(cheng)式(shi)(shi)(shi)(shi)，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代(dai)入(ru)方(fang)(fang)程(cheng)(cheng)式(shi)(shi)(shi)(shi)，計算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)，再乘以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)，即(ji)(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)。 七、試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)性(xing)(xing)(xing)能1.樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)(xian)性(xing)(xing)(xing)回(hui)(hui)歸(gui)與預(yu)(yu)期濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)相(xiang)(xiang)關(guan)系數(shu)(shu)(shu)R值(zhi)為(wei)(wei)(wei)0.92以(yi)(yi)(yi)(yi)(yi)(yi)上(shang)。2.批內(nei)(nei)(nei)(nei)(nei)與批見應(ying)(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)別(bie)(bie)小(xiao)(xiao)(xiao)于(yu)(yu)(yu)9%和15% 八、保(bao)(bao)存條(tiao)(tiao)件及(ji)(ji)(ji)有(you)(you)(you)(you)(you)效(xiao)期1.試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)保(bao)(bao)存：2-8℃。 2．有(you)(you)(you)(you)(you)效(xiao)期：6個(ge)月