牛細胞角蛋白(bai)13(CK-13/KRT13)ELISA 試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)(zhong)文(wen)(wen)名(ming)稱牛細胞角蛋白(bai)13(CK-13/KRT13)ELISA 試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)規格96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)類(lei)型(xing)血清(qing)、血漿、細胞上清(qing)液(ye)(ye)(ye)、尿(niao)液(ye)(ye)(ye)、體液(ye)(ye)(ye)、灌洗(xi)液(ye)(ye)(ye)等使用(yong)(yong)(yong)(yong)(yong)范(fan)圍科研實(shi)驗(yan)，不(bu)得(de)用(yong)(yong)(yong)(yong)(yong)于臨床(chuang)診斷反應(ying)(ying)(ying)時(shi)(shi)(shi)間(jian)1-5h試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)保(bao)(bao)(bao)存(cun)2-8℃有效期(qi)6個(ge)(ge)月貨期(qi)3-5天【操作(zuo)(zuo)步(bu)驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)的(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)與(yu)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)(zai)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被板(ban)(ban)(ban)(ban)上設(she)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)100μl，然(ran)(ran)后(hou)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)(yun)；然(ran)(ran)后(hou)從第(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)各(ge)(ge)(ge)取(qu)100μl分(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)第(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)(yun)；然(ran)(ran)后(hou)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)第(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)先(xian)各(ge)(ge)(ge)取(qu)50μl棄掉(diao)，再(zai)(zai)各(ge)(ge)(ge)取(qu)50μl分(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)50ul，混(hun)(hun)勻(yun)(yun)；混(hun)(hun)勻(yun)(yun)后(hou)從第(di)(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)各(ge)(ge)(ge)取(qu)50μl分(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)(yun)后(hou)從第(di)(di)(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分(fen)別(bie)取(qu)50μl加(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)九(jiu)、第(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)(yun)后(hou)從第(di)(di)(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)各(ge)(ge)(ge)取(qu)50μl棄掉(diao)。（稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)各(ge)(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)都為(wei)(wei)50μl，濃(nong)(nong)度(du)(du)(du)(du)分(fen)別(bie)為(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)別(bie)設(she)空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空白(bai)對(dui)照(zhao)(zhao)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)及(ji)(ji)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，其(qi)(qi)(qi)余各(ge)(ge)(ge)步(bu)操作(zuo)(zuo)相(xiang)同(tong)(tong)(tong)(tong)）、待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)(zai)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被板(ban)(ban)(ban)(ban)上待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)先(xian)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)40μl，然(ran)(ran)后(hou)再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)＊終(zhong)(zhong)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)度(du)(du)(du)(du)為(wei)(wei)5倍(bei)(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)于酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底(di)部，盡(jin)量(liang)(liang)不(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕輕晃動混(hun)(hun)勻(yun)(yun)。3. 溫(wen)育：用(yong)(yong)(yong)(yong)(yong)封(feng)板(ban)(ban)(ban)(ban)膜封(feng)板(ban)(ban)(ban)(ban)后(hou)置37℃溫(wen)育30分(fen)鐘(zhong)(zhong)。4. 配液(ye)(ye)(ye)：將(jiang)(jiang)(jiang)30（48T的(de)(de)20倍(bei)(bei)(bei)）倍(bei)(bei)(bei)濃(nong)(nong)縮洗(xi)滌液(ye)(ye)(ye)用(yong)(yong)(yong)(yong)(yong)蒸餾水30（48T的(de)(de)20倍(bei)(bei)(bei)）倍(bei)(bei)(bei)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)備用(yong)(yong)(yong)(yong)(yong)。5. 洗(xi)滌：小(xiao)心揭掉(diao)封(feng)板(ban)(ban)(ban)(ban)膜，棄去液(ye)(ye)(ye)體，甩干(gan)，每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)滿洗(xi)滌液(ye)(ye)(ye)，靜置30秒后(hou)棄去，如此重復(fu)5次，拍干(gan)。6. 加(jia)(jia)(jia)(jia)(jia)酶(mei)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)入酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)50μl，空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外(wai)。7. 溫(wen)育：操作(zuo)(zuo)同(tong)(tong)(tong)(tong)3。8. 洗(xi)滌：操作(zuo)(zuo)同(tong)(tong)(tong)(tong)5。9. 顯(xian)(xian)(xian)色(se)(se)(se)(se)(se)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)(jia)(jia)(jia)入顯(xian)(xian)(xian)色(se)(se)(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)(ji)A50μl，再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)入顯(xian)(xian)(xian)色(se)(se)(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)(ji)B50μl，輕輕震(zhen)蕩混(hun)(hun)勻(yun)(yun)，37℃避(bi)光顯(xian)(xian)(xian)色(se)(se)(se)(se)(se)15分(fen)鐘(zhong)(zhong). 10. 終(zhong)(zhong)止(zhi)(zhi)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)止(zhi)(zhi)液(ye)(ye)(ye)50μl，終(zhong)(zhong)止(zhi)(zhi)反應(ying)(ying)(ying)（此時(shi)(shi)(shi)藍色(se)(se)(se)(se)(se)立轉(zhuan)黃(huang)色(se)(se)(se)(se)(se)）。11. 測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)：以(yi)(yi)(yi)(yi)空白(bai)空調(diao)零，450nm波長依序測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)各(ge)(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)吸光度(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)應(ying)(ying)(ying)在(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)止(zhi)(zhi)液(ye)(ye)(ye)后(hou)15分(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)(yi)內(nei)(nei)進(jin)(jin)(jin)行(xing)。牛細胞角蛋白(bai)13(CK-13/KRT13)ELISA 試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)【注(zhu)意事項】1． 試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)從冷藏(zang)環(huan)境(jing)(jing)中(zhong)(zhong)(zhong)取(qu)出(chu)應(ying)(ying)(ying)在(zai)(zai)(zai)室溫(wen)平(ping)衡15-30分(fen)鐘(zhong)(zhong)后(hou)方(fang)可(ke)(ke)使用(yong)(yong)(yong)(yong)(yong)，酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被板(ban)(ban)(ban)(ban)開封(feng)后(hou)如未(wei)(wei)用(yong)(yong)(yong)(yong)(yong)完(wan)，板(ban)(ban)(ban)(ban)條應(ying)(ying)(ying)裝入密(mi)封(feng)袋(dai)中(zhong)(zhong)(zhong)保(bao)(bao)(bao)存(cun)。2． 濃(nong)(nong)洗(xi)滌液(ye)(ye)(ye)可(ke)(ke)能(neng)會有結晶析出(chu)，稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)可(ke)(ke)在(zai)(zai)(zai)水浴中(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)溫(wen)助(zhu)溶，洗(xi)滌時(shi)(shi)(shi)不(bu)影響(xiang)結果。3． 各(ge)(ge)(ge)步(bu)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)均(jun)應(ying)(ying)(ying)使用(yong)(yong)(yong)(yong)(yong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)器，并經常校對(dui)其(qi)(qi)(qi)準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)，以(yi)(yi)(yi)(yi)避(bi)免試(shi)(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)誤差(cha)(cha)。一(yi)(yi)次加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)間(jian)＊好控(kong)制(zhi)在(zai)(zai)(zai)5分(fen)鐘(zhong)(zhong)內(nei)(nei)，如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)數(shu)量(liang)(liang)多，推(tui)薦使用(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)。4． 請(qing)每(mei)(mei)次測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)的(de)(de)同(tong)(tong)(tong)(tong)時(shi)(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)，＊好做復(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)中(zhong)(zhong)(zhong)待測(ce)(ce)(ce)(ce)(ce)物(wu)(wu)質(zhi)(zhi)含(han)量(liang)(liang)過高(gao)（樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)OD值(zhi)(zhi)(zhi)(zhi)(zhi)大于標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)），請(qing)先(xian)用(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)定(ding)(ding)(ding)倍(bei)(bei)(bei)數(shu)（n倍(bei)(bei)(bei)）后(hou)再(zai)(zai)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，計(ji)算(suan)時(shi)(shi)(shi)請(qing)＊后(hou)乘(cheng)(cheng)以(yi)(yi)(yi)(yi)總稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)（×n×5）。5． 封(feng)板(ban)(ban)(ban)(ban)膜只限(xian)一(yi)(yi)次性(xing)使用(yong)(yong)(yong)(yong)(yong)，以(yi)(yi)(yi)(yi)避(bi)免交(jiao)叉污染。6． 底(di)物(wu)(wu)請(qing)避(bi)光保(bao)(bao)(bao)存(cun)。7． 嚴格按照(zhao)(zhao)說(shuo)(shuo)明書(shu)的(de)(de)操作(zuo)(zuo)進(jin)(jin)(jin)行(xing)，試(shi)(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)結果判定(ding)(ding)(ding)必須以(yi)(yi)(yi)(yi)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀數(shu)為(wei)(wei)準(zhun)(zhun)(zhun)(zhun)(zhun).8． 所(suo)有樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)，洗(xi)滌液(ye)(ye)(ye)和(he)各(ge)(ge)(ge)種廢(fei)棄物(wu)(wu)都應(ying)(ying)(ying)按傳染物(wu)(wu)處理。9． 本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)不(bu)同(tong)(tong)(tong)(tong)批(pi)號組分(fen)不(bu)得(de)混(hun)(hun)用(yong)(yong)(yong)(yong)(yong)。10. 如與(yu)英(ying)文(wen)(wen)說(shuo)(shuo)明書(shu)有異，以(yi)(yi)(yi)(yi)英(ying)文(wen)(wen)說(shuo)(shuo)明書(shu)為(wei)(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)。【計(ji)算(suan)】 以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)濃(nong)(nong)度(du)(du)(du)(du)為(wei)(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)(wei)縱(zong)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上繪(hui)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)，根據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)OD 值(zhi)(zhi)(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)查出(chu)相(xiang)應(ying)(ying)(ying)的(de)(de)濃(nong)(nong)度(du)(du)(du)(du)；再(zai)(zai)乘(cheng)(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi) 倍(bei)(bei)(bei)數(shu)；或用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)濃(nong)(nong)度(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)(zhi)(zhi)計(ji)算(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)的(de)(de)直(zhi)線(xian)回(hui)歸方(fang)程式(shi)，將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi) 代入方(fang)程式(shi)，計(ji)算(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)，再(zai)(zai)乘(cheng)(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi) 倍(bei)(bei)(bei)數(shu)，即為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)實(shi)際濃(nong)(nong)度(du)(du)(du)(du)。 【試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)組成】試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)組成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置保(bao)(bao)(bao)存(cun)說(shuo)(shuo)明書(shu)1份(fen)1份(fen)密(mi)封(feng)袋(dai)1個(ge)(ge)1個(ge)(ge)封(feng)板(ban)(ban)(ban)(ban)膜12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條2片（96）2-8℃保(bao)(bao)(bao)存(cun)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條2-8℃保(bao)(bao)(bao)存(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)0.3mL*6管(guan)0.3mL*6管(guan)2-8℃保(bao)(bao)(bao)存(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)顯(xian)(xian)(xian)色(se)(se)(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)顯(xian)(xian)(xian)色(se)(se)(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)終(zhong)(zhong)止(zhi)(zhi)液(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)注(zhu)意：使用(yong)(yong)(yong)(yong)(yong)前請(qing)檢(jian)(jian)查試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和(he)數(shu)量(liang)(liang)與(yu)表(biao)格是否一(yi)(yi)致(zhi)。【精密(mi)度(du)(du)(du)(du)】精密(mi)度(du)(du)(du)(du)用(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)變異系數(shu)CV 表(biao)示。CV（%） = SD/mean×100批(pi)內(nei)(nei)差(cha)(cha)：取(qu)同(tong)(tong)(tong)(tong)批(pi)次試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)對(dui)低、中(zhong)(zhong)(zhong)、高(gao)值(zhi)(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)進(jin)(jin)(jin)行(xing)定(ding)(ding)(ding)量(liang)(liang)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，每(mei)(mei)份(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)連續(xu)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)20 次，分(fen)別(bie)計(ji)算(suan)不(bu)同(tong)(tong)(tong)(tong)濃(nong)(nong)度(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)平(ping)均(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)(zhi)(zhi)。批(pi)內(nei)(nei)差(cha)(cha)： CV<9%批(pi)間(jian)差(cha)(cha)：選取(qu)3 個(ge)(ge)不(bu)同(tong)(tong)(tong)(tong)批(pi)次的(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)分(fen)別(bie)對(dui)低、中(zhong)(zhong)(zhong)、高(gao)值(zhi)(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)進(jin)(jin)(jin)行(xing)定(ding)(ding)(ding)量(liang)(liang)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，每(mei)(mei)個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)使用(yong)(yong)(yong)(yong)(yong)同(tong)(tong)(tong)(tong)一(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)重復(fu)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)10 次，分(fen)別(bie)計(ji)算(suan)不(bu)同(tong)(tong)(tong)(tong)濃(nong)(nong)度(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)平(ping)均(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)(zhi)(zhi)。批(pi)間(jian)差(cha)(cha)： CV<11% 【特異性(xing)】本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)識別(bie)天然(ran)(ran)和(he)重組微生物(wu)(wu)硝酸鹽還原酶(mei)（Nar），經檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)與(yu)其(qi)(qi)(qi)它相(xiang)似(si)物(wu)(wu)質(zhi)(zhi)無(wu)明顯(xian)(xian)(xian)交(jiao)叉反應(ying)(ying)(ying)。由(you)于受到(dao)技術及(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)來(lai)源的(de)(de)限(xian)制(zhi)，不(bu)可(ke)(ke)能(neng)完(wan)成所(suo)有相(xiang)關或相(xiang)似(si)物(wu)(wu)質(zhi)(zhi)交(jiao)叉反應(ying)(ying)(ying)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，因此本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)有可(ke)(ke)能(neng)與(yu)未(wei)(wei)經檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)其(qi)(qi)(qi)它物(wu)(wu)質(zhi)(zhi)有交(jiao)叉反應(ying)(ying)(ying)。 【穩(wen)定(ding)(ding)(ding)性(xing)】試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)在(zai)(zai)(zai)有效期(qi)內(nei)(nei)務必按推(tui)薦溫(wen)度(du)(du)(du)(du)保(bao)(bao)(bao)存(cun)，活性(xing)降低率小(xiao)于5%。為(wei)(wei)減(jian)小(xiao)外(wai)部因素對(dui)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)破壞(huai)前后(hou)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)值(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)影響(xiang)，實(shi)驗(yan)室的(de)(de)環(huan)境(jing)(jing)條件需盡(jin)量(liang)(liang)保(bao)(bao)(bao)持(chi)一(yi)(yi)致(zhi)，尤其(qi)(qi)(qi)是實(shi)驗(yan)室內(nei)(nei)溫(wen)度(du)(du)(du)(du)、濕度(du)(du)(du)(du)及(ji)(ji)溫(wen)育條件。其(qi)(qi)(qi)次由(you)同(tong)(tong)(tong)(tong)一(yi)(yi)實(shi)驗(yan)員來(lai)進(jin)(jin)(jin)行(xing)操作(zuo)(zuo)可(ke)(ke)減(jian)少(shao)人為(wei)(wei)誤差(cha)(cha)。
牛(niu)細(xi)胞毒(du)性(xing)(xing)T淋巴(ba)細(xi)胞相(xiang)(xiang)關抗原4(CTLA4)ELISA 試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)文(wen)(wen)名(ming)稱牛(niu)細(xi)胞毒(du)性(xing)(xing)T淋巴(ba)細(xi)胞相(xiang)(xiang)關抗原4(CTLA4)ELISA 試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)規格(ge)96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)類型(xing)血清(qing)(qing)、血漿、細(xi)胞上(shang)清(qing)(qing)液(ye)(ye)(ye)、尿液(ye)(ye)(ye)、體液(ye)(ye)(ye)、灌(guan)洗(xi)(xi)液(ye)(ye)(ye)等使用(yong)(yong)(yong)(yong)范圍科研實驗(yan)，不(bu)(bu)得用(yong)(yong)(yong)(yong)于臨床診斷(duan)反應(ying)時(shi)(shi)間1-5h試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)保(bao)(bao)(bao)存2-8℃有效期(qi)6個(ge)月(yue)貨期(qi)3-5天【操作步(bu)驟(zou)】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)的(de)(de)(de)(de)稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)與(yu)(yu)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)(zai)(zai)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)上(shang)設標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)，在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)一(yi)、第(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)100μl，然(ran)(ran)后(hou)(hou)(hou)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)一(yi)、第(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)50μl，混勻(yun)(yun)(yun)(yun)；然(ran)(ran)后(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)一(yi)孔(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各取100μl分(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)，再(zai)(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)分(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)50μl，混勻(yun)(yun)(yun)(yun)；然(ran)(ran)后(hou)(hou)(hou)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)先各取50μl棄(qi)掉(diao)，再(zai)(zai)(zai)各取50μl分(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)50ul，混勻(yun)(yun)(yun)(yun)；混勻(yun)(yun)(yun)(yun)后(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各取50μl分(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八(ba)(ba)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八(ba)(ba)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)50μl，混勻(yun)(yun)(yun)(yun)后(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八(ba)(ba)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)(bie)(bie)取50μl加(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)九、第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)分(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)50μl，混勻(yun)(yun)(yun)(yun)后(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各取50μl棄(qi)掉(diao)。（稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)各孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)(liang)(liang)都為(wei)(wei)(wei)50μl，濃(nong)(nong)度(du)(du)分(fen)(fen)別(bie)(bie)(bie)(bie)(bie)為(wei)(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)別(bie)(bie)(bie)(bie)(bie)設空白孔(kong)(kong)(kong)(kong)（空白對(dui)照孔(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)及(ji)(ji)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)，其(qi)(qi)余各步(bu)操作相(xiang)(xiang)同(tong)(tong)）、待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)。在(zai)(zai)(zai)(zai)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)上(shang)待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)先加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)40μl，然(ran)(ran)后(hou)(hou)(hou)再(zai)(zai)(zai)加(jia)(jia)(jia)(jia)待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)＊終(zhong)稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)度(du)(du)為(wei)(wei)(wei)5倍(bei)）。加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)加(jia)(jia)(jia)(jia)于酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)(kong)(kong)(kong)底(di)部，盡量(liang)(liang)(liang)(liang)不(bu)(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)壁，輕輕晃動混勻(yun)(yun)(yun)(yun)。3. 溫(wen)育：用(yong)(yong)(yong)(yong)封(feng)(feng)(feng)板(ban)(ban)膜(mo)封(feng)(feng)(feng)板(ban)(ban)后(hou)(hou)(hou)置37℃溫(wen)育30分(fen)(fen)鐘(zhong)。4. 配液(ye)(ye)(ye)：將(jiang)(jiang)30（48T的(de)(de)(de)(de)20倍(bei)）倍(bei)濃(nong)(nong)縮洗(xi)(xi)滌(di)液(ye)(ye)(ye)用(yong)(yong)(yong)(yong)蒸餾水(shui)30（48T的(de)(de)(de)(de)20倍(bei)）倍(bei)稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)備用(yong)(yong)(yong)(yong)。5. 洗(xi)(xi)滌(di)：小心揭掉(diao)封(feng)(feng)(feng)板(ban)(ban)膜(mo)，棄(qi)去液(ye)(ye)(ye)體，甩干，每(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿洗(xi)(xi)滌(di)液(ye)(ye)(ye)，靜置30秒后(hou)(hou)(hou)棄(qi)去，如此重復5次，拍干。6. 加(jia)(jia)(jia)(jia)酶：每(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)50μl，空白孔(kong)(kong)(kong)(kong)除外。7. 溫(wen)育：操作同(tong)(tong)3。8. 洗(xi)(xi)滌(di)：操作同(tong)(tong)5。9. 顯(xian)色(se)：每(mei)孔(kong)(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)入顯(xian)色(se)劑(ji)(ji)(ji)A50μl，再(zai)(zai)(zai)加(jia)(jia)(jia)(jia)入顯(xian)色(se)劑(ji)(ji)(ji)B50μl，輕輕震蕩混勻(yun)(yun)(yun)(yun)，37℃避光(guang)顯(xian)色(se)15分(fen)(fen)鐘(zhong). 10. 終(zhong)止：每(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)50μl，終(zhong)止反應(ying)（此時(shi)(shi)藍色(se)立轉黃色(se)）。11. 測(ce)(ce)(ce)定(ding)(ding)：以空白空調零，450nm波長依序測(ce)(ce)(ce)量(liang)(liang)(liang)(liang)各孔(kong)(kong)(kong)(kong)的(de)(de)(de)(de)吸(xi)光(guang)度(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)定(ding)(ding)應(ying)在(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)鐘(zhong)以內(nei)(nei)進(jin)(jin)行(xing)(xing)。牛(niu)細(xi)胞毒(du)性(xing)(xing)T淋巴(ba)細(xi)胞相(xiang)(xiang)關抗原4(CTLA4)ELISA 試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)【注意(yi)事項】1． 試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)從(cong)冷(leng)藏(zang)環境中(zhong)(zhong)(zhong)(zhong)(zhong)取出應(ying)在(zai)(zai)(zai)(zai)室溫(wen)平(ping)衡15-30分(fen)(fen)鐘(zhong)后(hou)(hou)(hou)方(fang)(fang)可(ke)使用(yong)(yong)(yong)(yong)，酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)開封(feng)(feng)(feng)后(hou)(hou)(hou)如未用(yong)(yong)(yong)(yong)完(wan)，板(ban)(ban)條(tiao)應(ying)裝入密(mi)封(feng)(feng)(feng)袋中(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)(bao)存。2． 濃(nong)(nong)洗(xi)(xi)滌(di)液(ye)(ye)(ye)可(ke)能會有結(jie)晶析(xi)出，稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)可(ke)在(zai)(zai)(zai)(zai)水(shui)浴中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)溫(wen)助溶，洗(xi)(xi)滌(di)時(shi)(shi)不(bu)(bu)影響結(jie)果。3． 各步(bu)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)均(jun)應(ying)使用(yong)(yong)(yong)(yong)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)器，并經(jing)常校對(dui)其(qi)(qi)準(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)，以避免試(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)誤(wu)差(cha)(cha)。一(yi)次加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)間＊好控制在(zai)(zai)(zai)(zai)5分(fen)(fen)鐘(zhong)內(nei)(nei)，如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)數(shu)量(liang)(liang)(liang)(liang)多，推薦使用(yong)(yong)(yong)(yong)排(pai)槍加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)。4． 請(qing)(qing)每(mei)次測(ce)(ce)(ce)定(ding)(ding)的(de)(de)(de)(de)同(tong)(tong)時(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲線(xian)，＊好做復孔(kong)(kong)(kong)(kong)。如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)待測(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)(liang)(liang)(liang)過高(gao)(gao)（樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)大于標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)一(yi)孔(kong)(kong)(kong)(kong)的(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)），請(qing)(qing)先用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)一(yi)定(ding)(ding)倍(bei)數(shu)（n倍(bei)）后(hou)(hou)(hou)再(zai)(zai)(zai)測(ce)(ce)(ce)定(ding)(ding)，計(ji)(ji)算時(shi)(shi)請(qing)(qing)＊后(hou)(hou)(hou)乘以總稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)（×n×5）。5． 封(feng)(feng)(feng)板(ban)(ban)膜(mo)只限一(yi)次性(xing)(xing)使用(yong)(yong)(yong)(yong)，以避免交叉(cha)污染(ran)。6． 底(di)物(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)避光(guang)保(bao)(bao)(bao)存。7． 嚴(yan)格(ge)按照說明書(shu)(shu)的(de)(de)(de)(de)操作進(jin)(jin)行(xing)(xing)，試(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)結(jie)果判定(ding)(ding)必(bi)(bi)須以酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀數(shu)為(wei)(wei)(wei)準(zhun)(zhun)(zhun)(zhun).8． 所(suo)有樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)，洗(xi)(xi)滌(di)液(ye)(ye)(ye)和各種廢棄(qi)物(wu)(wu)(wu)(wu)(wu)(wu)都應(ying)按傳染(ran)物(wu)(wu)(wu)(wu)(wu)(wu)處理。9． 本(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)不(bu)(bu)同(tong)(tong)批(pi)號組分(fen)(fen)不(bu)(bu)得混用(yong)(yong)(yong)(yong)。10. 如與(yu)(yu)英(ying)文(wen)(wen)說明書(shu)(shu)有異，以英(ying)文(wen)(wen)說明書(shu)(shu)為(wei)(wei)(wei)準(zhun)(zhun)(zhun)(zhun)。【計(ji)(ji)算】 以標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)濃(nong)(nong)度(du)(du)為(wei)(wei)(wei)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)(wei)(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)(zai)(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)繪出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲線(xian)，根據樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)OD 值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)由(you)(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲線(xian)查(cha)出相(xiang)(xiang)應(ying)的(de)(de)(de)(de)濃(nong)(nong)度(du)(du)；再(zai)(zai)(zai)乘以稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi) 倍(bei)數(shu)；或(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)濃(nong)(nong)度(du)(du)與(yu)(yu)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)計(ji)(ji)算出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)曲線(xian)的(de)(de)(de)(de)直線(xian)回歸方(fang)(fang)程式，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi) 代(dai)入方(fang)(fang)程式，計(ji)(ji)算出樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)濃(nong)(nong)度(du)(du)，再(zai)(zai)(zai)乘以稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi) 倍(bei)數(shu)，即為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)實際濃(nong)(nong)度(du)(du)。 【試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)組成】試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)組成48孔(kong)(kong)(kong)(kong)配置96孔(kong)(kong)(kong)(kong)配置保(bao)(bao)(bao)存說明書(shu)(shu)1份1份密(mi)封(feng)(feng)(feng)袋1個(ge)1個(ge)封(feng)(feng)(feng)板(ban)(ban)膜(mo)12孔(kong)(kong)(kong)(kong)×4條(tiao)2片（96）2-8℃保(bao)(bao)(bao)存微(wei)孔(kong)(kong)(kong)(kong)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)×8條(tiao)2-8℃保(bao)(bao)(bao)存標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)(bao)存標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存顯(xian)色(se)劑(ji)(ji)(ji)A液(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存顯(xian)色(se)劑(ji)(ji)(ji)B液(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存終(zhong)止液(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存注意(yi)：使用(yong)(yong)(yong)(yong)前請(qing)(qing)檢查(cha)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)的(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和數(shu)量(liang)(liang)(liang)(liang)與(yu)(yu)表格(ge)是否(fou)一(yi)致。【精密(mi)度(du)(du)】精密(mi)度(du)(du)用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)測(ce)(ce)(ce)定(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)變異系數(shu)CV 表示。CV（%） = SD/mean×100批(pi)內(nei)(nei)差(cha)(cha)：取同(tong)(tong)批(pi)次試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)對(dui)低(di)、中(zhong)(zhong)(zhong)(zhong)(zhong)、高(gao)(gao)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)定(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)進(jin)(jin)行(xing)(xing)定(ding)(ding)量(liang)(liang)(liang)(liang)檢測(ce)(ce)(ce)，每(mei)份樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)連續測(ce)(ce)(ce)定(ding)(ding)20 次，分(fen)(fen)別(bie)(bie)(bie)(bie)(bie)計(ji)(ji)算不(bu)(bu)同(tong)(tong)濃(nong)(nong)度(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)(de)(de)平(ping)均(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。批(pi)內(nei)(nei)差(cha)(cha)： CV<9%批(pi)間差(cha)(cha)：選取3 個(ge)不(bu)(bu)同(tong)(tong)批(pi)次的(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)分(fen)(fen)別(bie)(bie)(bie)(bie)(bie)對(dui)低(di)、中(zhong)(zhong)(zhong)(zhong)(zhong)、高(gao)(gao)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)定(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)進(jin)(jin)行(xing)(xing)定(ding)(ding)量(liang)(liang)(liang)(liang)測(ce)(ce)(ce)定(ding)(ding)，每(mei)個(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)使用(yong)(yong)(yong)(yong)同(tong)(tong)一(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)重復測(ce)(ce)(ce)定(ding)(ding)10 次，分(fen)(fen)別(bie)(bie)(bie)(bie)(bie)計(ji)(ji)算不(bu)(bu)同(tong)(tong)濃(nong)(nong)度(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)(de)(de)平(ping)均(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。批(pi)間差(cha)(cha)： CV<11% 【特異性(xing)(xing)】本(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)識別(bie)(bie)(bie)(bie)(bie)天然(ran)(ran)和重組微(wei)生物(wu)(wu)(wu)(wu)(wu)(wu)硝酸鹽還原酶（Nar），經(jing)檢測(ce)(ce)(ce)與(yu)(yu)其(qi)(qi)它(ta)(ta)相(xiang)(xiang)似(si)物(wu)(wu)(wu)(wu)(wu)(wu)質無明顯(xian)交叉(cha)反應(ying)。由(you)(you)于受到技術(shu)及(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)來(lai)源的(de)(de)(de)(de)限制，不(bu)(bu)可(ke)能完(wan)成所(suo)有相(xiang)(xiang)關或(huo)相(xiang)(xiang)似(si)物(wu)(wu)(wu)(wu)(wu)(wu)質交叉(cha)反應(ying)檢測(ce)(ce)(ce)，因此本(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)有可(ke)能與(yu)(yu)未經(jing)檢測(ce)(ce)(ce)的(de)(de)(de)(de)其(qi)(qi)它(ta)(ta)物(wu)(wu)(wu)(wu)(wu)(wu)質有交叉(cha)反應(ying)。 【穩定(ding)(ding)性(xing)(xing)】試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)在(zai)(zai)(zai)(zai)有效期(qi)內(nei)(nei)務必(bi)(bi)按推薦溫(wen)度(du)(du)保(bao)(bao)(bao)存，活性(xing)(xing)降低(di)率小于5%。為(wei)(wei)(wei)減(jian)小外部因素對(dui)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)破壞前后(hou)(hou)(hou)檢測(ce)(ce)(ce)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)影響，實驗(yan)室的(de)(de)(de)(de)環境條(tiao)件(jian)需盡量(liang)(liang)(liang)(liang)保(bao)(bao)(bao)持一(yi)致，尤其(qi)(qi)是實驗(yan)室內(nei)(nei)溫(wen)度(du)(du)、濕(shi)度(du)(du)及(ji)(ji)溫(wen)育條(tiao)件(jian)。其(qi)(qi)次由(you)(you)同(tong)(tong)一(yi)實驗(yan)員(yuan)來(lai)進(jin)(jin)行(xing)(xing)操作可(ke)減(jian)少人(ren)為(wei)(wei)(wei)誤(wu)差(cha)(cha)。
牛細(xi)胞(bao)毒素(su)(su)相(xiang)關(guan)(guan)(guan)蛋(dan)白(bai)A(CAPA)ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)文名稱牛細(xi)胞(bao)毒素(su)(su)相(xiang)關(guan)(guan)(guan)蛋(dan)白(bai)A(CAPA)ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)規格96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)類型血清、血漿、細(xi)胞(bao)上(shang)清液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、體(ti)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、灌洗(xi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)等使(shi)用(yong)(yong)(yong)(yong)(yong)范(fan)圍科(ke)研實驗，不(bu)(bu)(bu)得用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)臨床診斷反(fan)應(ying)(ying)(ying)時(shi)(shi)(shi)(shi)間(jian)1-5h試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)保存2-8℃有效(xiao)期(qi)(qi)6個月(yue)貨期(qi)(qi)3-5天(tian)【操作(zuo)步(bu)驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)與(yu)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)(zai)(zai)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被(bei)(bei)板(ban)(ban)(ban)上(shang)設標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)100μl，然(ran)(ran)(ran)后(hou)(hou)(hou)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)；然(ran)(ran)(ran)后(hou)(hou)(hou)從第(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取100μl分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)第(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)三、第(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)；然(ran)(ran)(ran)后(hou)(hou)(hou)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)第(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)先(xian)各(ge)(ge)取50μl棄(qi)掉(diao)，再(zai)(zai)(zai)(zai)各(ge)(ge)取50μl分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50ul，混(hun)勻(yun)；混(hun)勻(yun)后(hou)(hou)(hou)從第(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取50μl分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)后(hou)(hou)(hou)從第(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)取50μl加(jia)(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)(di)(di)九、第(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)后(hou)(hou)(hou)從第(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取50μl棄(qi)掉(diao)。（稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)量都為(wei)(wei)(wei)(wei)50μl，濃(nong)(nong)度(du)(du)(du)(du)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)為(wei)(wei)(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)設空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)白(bai)對照(zhao)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)及(ji)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，其(qi)(qi)(qi)余(yu)各(ge)(ge)步(bu)操作(zuo)相(xiang)同(tong)(tong)(tong)(tong)）、待(dai)測(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)(zai)(zai)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被(bei)(bei)板(ban)(ban)(ban)上(shang)待(dai)測(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)先(xian)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)40μl，然(ran)(ran)(ran)后(hou)(hou)(hou)再(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)待(dai)測(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)＊終(zhong)(zhong)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)度(du)(du)(du)(du)為(wei)(wei)(wei)(wei)5倍(bei)(bei)）。加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)于(yu)(yu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部，盡量不(bu)(bu)(bu)觸及(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)(qing)輕(qing)(qing)晃動混(hun)勻(yun)。3. 溫(wen)育(yu)：用(yong)(yong)(yong)(yong)(yong)封(feng)板(ban)(ban)(ban)膜(mo)(mo)封(feng)板(ban)(ban)(ban)后(hou)(hou)(hou)置(zhi)37℃溫(wen)育(yu)30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)。4. 配(pei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)：將30（48T的(de)(de)(de)(de)(de)20倍(bei)(bei)）倍(bei)(bei)濃(nong)(nong)縮洗(xi)滌液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)用(yong)(yong)(yong)(yong)(yong)蒸餾水(shui)30（48T的(de)(de)(de)(de)(de)20倍(bei)(bei)）倍(bei)(bei)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)備用(yong)(yong)(yong)(yong)(yong)。5. 洗(xi)滌：小(xiao)心(xin)揭掉(diao)封(feng)板(ban)(ban)(ban)膜(mo)(mo)，棄(qi)去液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，甩干，每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿洗(xi)滌液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，靜置(zhi)30秒后(hou)(hou)(hou)棄(qi)去，如(ru)此(ci)重復(fu)5次(ci)，拍干。6. 加(jia)(jia)(jia)(jia)酶(mei)：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)(ru)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)50μl，空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外(wai)。7. 溫(wen)育(yu)：操作(zuo)同(tong)(tong)(tong)(tong)3。8. 洗(xi)滌：操作(zuo)同(tong)(tong)(tong)(tong)5。9. 顯(xian)(xian)(xian)(xian)(xian)色(se)：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)(jia)(jia)入(ru)(ru)顯(xian)(xian)(xian)(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)A50μl，再(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)入(ru)(ru)顯(xian)(xian)(xian)(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)B50μl，輕(qing)(qing)輕(qing)(qing)震蕩混(hun)勻(yun)，37℃避光(guang)顯(xian)(xian)(xian)(xian)(xian)色(se)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong). 10. 終(zhong)(zhong)止(zhi)：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)終(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)止(zhi)反(fan)應(ying)(ying)(ying)（此(ci)時(shi)(shi)(shi)(shi)藍色(se)立轉黃色(se)）。11. 測(ce)定(ding)：以(yi)(yi)(yi)空(kong)白(bai)空(kong)調零，450nm波(bo)長依序(xu)測(ce)量各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)吸光(guang)度(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)）。 測(ce)定(ding)應(ying)(ying)(ying)在(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)終(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)以(yi)(yi)(yi)內(nei)進行(xing)。牛細(xi)胞(bao)毒素(su)(su)相(xiang)關(guan)(guan)(guan)蛋(dan)白(bai)A(CAPA)ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)【注意事(shi)項(xiang)】1． 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)從冷(leng)藏環境中(zhong)(zhong)(zhong)(zhong)(zhong)取出(chu)(chu)(chu)應(ying)(ying)(ying)在(zai)(zai)(zai)(zai)室(shi)溫(wen)平衡15-30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)后(hou)(hou)(hou)方(fang)可(ke)使(shi)用(yong)(yong)(yong)(yong)(yong)，酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被(bei)(bei)板(ban)(ban)(ban)開封(feng)后(hou)(hou)(hou)如(ru)未用(yong)(yong)(yong)(yong)(yong)完，板(ban)(ban)(ban)條(tiao)應(ying)(ying)(ying)裝(zhuang)入(ru)(ru)密(mi)(mi)封(feng)袋中(zhong)(zhong)(zhong)(zhong)(zhong)保存。2． 濃(nong)(nong)洗(xi)滌液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)可(ke)能會(hui)有結晶析出(chu)(chu)(chu)，稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)可(ke)在(zai)(zai)(zai)(zai)水(shui)浴中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)溫(wen)助溶，洗(xi)滌時(shi)(shi)(shi)(shi)不(bu)(bu)(bu)影(ying)響(xiang)結果。3． 各(ge)(ge)步(bu)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)均(jun)應(ying)(ying)(ying)使(shi)用(yong)(yong)(yong)(yong)(yong)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)器，并經(jing)常校對其(qi)(qi)(qi)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性，以(yi)(yi)(yi)避免(mian)試(shi)(shi)(shi)(shi)(shi)驗誤(wu)差(cha)。一(yi)(yi)次(ci)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)間(jian)＊好(hao)控制在(zai)(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)鐘(zhong)內(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)數量多(duo)，推薦(jian)使(shi)用(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4． 請(qing)每次(ci)測(ce)定(ding)的(de)(de)(de)(de)(de)同(tong)(tong)(tong)(tong)時(shi)(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)，＊好(hao)做(zuo)復(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)待(dai)測(ce)物(wu)(wu)質含量過高(gao)（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)OD值(zhi)(zhi)(zhi)大(da)于(yu)(yu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)），請(qing)先(xian)用(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)一(yi)(yi)定(ding)倍(bei)(bei)數（n倍(bei)(bei)）后(hou)(hou)(hou)再(zai)(zai)(zai)(zai)測(ce)定(ding)，計算(suan)時(shi)(shi)(shi)(shi)請(qing)＊后(hou)(hou)(hou)乘(cheng)以(yi)(yi)(yi)總稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數（×n×5）。5． 封(feng)板(ban)(ban)(ban)膜(mo)(mo)只限(xian)一(yi)(yi)次(ci)性使(shi)用(yong)(yong)(yong)(yong)(yong)，以(yi)(yi)(yi)避免(mian)交叉(cha)(cha)(cha)污染。6． 底物(wu)(wu)請(qing)避光(guang)保存。7． 嚴格按照(zhao)說明(ming)書(shu)的(de)(de)(de)(de)(de)操作(zuo)進行(xing)，試(shi)(shi)(shi)(shi)(shi)驗結果判(pan)定(ding)必(bi)須以(yi)(yi)(yi)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀(du)數為(wei)(wei)(wei)(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun).8． 所有樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)，洗(xi)滌液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)和(he)(he)各(ge)(ge)種廢棄(qi)物(wu)(wu)都應(ying)(ying)(ying)按傳(chuan)染物(wu)(wu)處(chu)理。9． 本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)不(bu)(bu)(bu)同(tong)(tong)(tong)(tong)批(pi)(pi)(pi)號組(zu)分(fen)(fen)(fen)(fen)(fen)不(bu)(bu)(bu)得混(hun)用(yong)(yong)(yong)(yong)(yong)。10. 如(ru)與(yu)英文說明(ming)書(shu)有異，以(yi)(yi)(yi)英文說明(ming)書(shu)為(wei)(wei)(wei)(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)。【計算(suan)】 以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)(zai)(zai)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)繪出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)，根據樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)OD 值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)查出(chu)(chu)(chu)相(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)；再(zai)(zai)(zai)(zai)乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi) 倍(bei)(bei)數；或用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計算(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)的(de)(de)(de)(de)(de)直線(xian)(xian)回(hui)歸方(fang)程(cheng)式(shi)(shi)，將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi) 代入(ru)(ru)方(fang)程(cheng)式(shi)(shi)，計算(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)，再(zai)(zai)(zai)(zai)乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi) 倍(bei)(bei)數，即為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)實際濃(nong)(nong)度(du)(du)(du)(du)。 【試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)組(zu)成】試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)組(zu)成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)保存說明(ming)書(shu)1份(fen)1份(fen)密(mi)(mi)封(feng)袋1個1個封(feng)板(ban)(ban)(ban)膜(mo)(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)2片（96）2-8℃保存微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)2-8℃保存標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)0.3mL*6管(guan)0.3mL*6管(guan)2-8℃保存標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)1.5ml×1瓶(ping)(ping)2-8℃保存酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)6 ml×1瓶(ping)(ping)2-8℃保存樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)6 ml×1瓶(ping)(ping)2-8℃保存顯(xian)(xian)(xian)(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)6 ml×1瓶(ping)(ping)2-8℃保存顯(xian)(xian)(xian)(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)6 ml×1瓶(ping)(ping)2-8℃保存終(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)6ml×1瓶(ping)(ping)2-8℃保存注意：使(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)請(qing)檢(jian)(jian)查試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和(he)(he)數量與(yu)表格是否一(yi)(yi)致。【精(jing)密(mi)(mi)度(du)(du)(du)(du)】精(jing)密(mi)(mi)度(du)(du)(du)(du)用(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)測(ce)定(ding)值(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)變異系數CV 表示。CV（%） = SD/mean×100批(pi)(pi)(pi)內(nei)差(cha)：取同(tong)(tong)(tong)(tong)批(pi)(pi)(pi)次(ci)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)對低(di)(di)、中(zhong)(zhong)(zhong)(zhong)(zhong)、高(gao)值(zhi)(zhi)(zhi)定(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)進行(xing)定(ding)量檢(jian)(jian)測(ce)，每份(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)連續(xu)測(ce)定(ding)20 次(ci)，分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)計算(suan)不(bu)(bu)(bu)同(tong)(tong)(tong)(tong)濃(nong)(nong)度(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)平均(jun)值(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)。批(pi)(pi)(pi)內(nei)差(cha)： CV<9%批(pi)(pi)(pi)間(jian)差(cha)：選取3 個不(bu)(bu)(bu)同(tong)(tong)(tong)(tong)批(pi)(pi)(pi)次(ci)的(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)對低(di)(di)、中(zhong)(zhong)(zhong)(zhong)(zhong)、高(gao)值(zhi)(zhi)(zhi)定(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)進行(xing)定(ding)量測(ce)定(ding)，每個樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)使(shi)用(yong)(yong)(yong)(yong)(yong)同(tong)(tong)(tong)(tong)一(yi)(yi)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)重復(fu)測(ce)定(ding)10 次(ci)，分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)計算(suan)不(bu)(bu)(bu)同(tong)(tong)(tong)(tong)濃(nong)(nong)度(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)平均(jun)值(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)。批(pi)(pi)(pi)間(jian)差(cha)： CV<11% 【特異性】本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)識別(bie)(bie)(bie)(bie)(bie)(bie)天(tian)然(ran)(ran)(ran)和(he)(he)重組(zu)微生物(wu)(wu)硝(xiao)酸(suan)鹽還原(yuan)酶(mei)（Nar），經(jing)檢(jian)(jian)測(ce)與(yu)其(qi)(qi)(qi)它(ta)相(xiang)似(si)物(wu)(wu)質無明(ming)顯(xian)(xian)(xian)(xian)(xian)交叉(cha)(cha)(cha)反(fan)應(ying)(ying)(ying)。由于(yu)(yu)受到(dao)(dao)技術(shu)及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)來源的(de)(de)(de)(de)(de)限(xian)制，不(bu)(bu)(bu)可(ke)能完成所有相(xiang)關(guan)(guan)(guan)或相(xiang)似(si)物(wu)(wu)質交叉(cha)(cha)(cha)反(fan)應(ying)(ying)(ying)檢(jian)(jian)測(ce)，因(yin)此(ci)本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)有可(ke)能與(yu)未經(jing)檢(jian)(jian)測(ce)的(de)(de)(de)(de)(de)其(qi)(qi)(qi)它(ta)物(wu)(wu)質有交叉(cha)(cha)(cha)反(fan)應(ying)(ying)(ying)。 【穩定(ding)性】試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)在(zai)(zai)(zai)(zai)有效(xiao)期(qi)(qi)內(nei)務必(bi)按推薦(jian)溫(wen)度(du)(du)(du)(du)保存，活性降(jiang)低(di)(di)率小(xiao)于(yu)(yu)5%。為(wei)(wei)(wei)(wei)減(jian)小(xiao)外(wai)部因(yin)素(su)(su)對試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)破(po)壞(huai)前(qian)后(hou)(hou)(hou)檢(jian)(jian)測(ce)值(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)影(ying)響(xiang)，實驗室(shi)的(de)(de)(de)(de)(de)環境條(tiao)件需盡量保持一(yi)(yi)致，尤其(qi)(qi)(qi)是實驗室(shi)內(nei)溫(wen)度(du)(du)(du)(du)、濕(shi)度(du)(du)(du)(du)及(ji)溫(wen)育(yu)條(tiao)件。其(qi)(qi)(qi)次(ci)由同(tong)(tong)(tong)(tong)一(yi)(yi)實驗員(yuan)來進行(xing)操作(zuo)可(ke)減(jian)少人(ren)為(wei)(wei)(wei)(wei)誤(wu)差(cha)。
牛基(ji)(ji)質(zhi)金屬蛋白(bai)(bai)酶(mei)(mei)(mei)(mei)23B(MMP23B)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)文(wen)名(ming)稱牛基(ji)(ji)質(zhi)金屬蛋白(bai)(bai)酶(mei)(mei)(mei)(mei)23B(MMP23B)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)規格(ge)96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)類型血清、血漿(jiang)、細胞上清液(ye)(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)(ye)、體(ti)液(ye)(ye)(ye)(ye)(ye)、灌洗(xi)(xi)(xi)液(ye)(ye)(ye)(ye)(ye)等使(shi)(shi)用(yong)(yong)(yong)范圍科(ke)研實(shi)(shi)驗(yan)(yan)，不(bu)(bu)得(de)(de)用(yong)(yong)(yong)于(yu)臨床診斷(duan)反應(ying)(ying)時(shi)(shi)間(jian)1-5h試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)2-8℃有(you)(you)效期(qi)6個(ge)月貨期(qi)3-5天【操作步驟】1. 標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)稀(xi)釋(shi)(shi)與加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：在酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)包(bao)(bao)被(bei)板(ban)(ban)上設標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在第(di)(di)(di)(di)(di)一、第(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)100μl，然(ran)(ran)(ran)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)在第(di)(di)(di)(di)(di)一、第(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)；然(ran)(ran)(ran)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)從第(di)(di)(di)(di)(di)一孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)(ge)(ge)取100μl分(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)(zai)在第(di)(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)；然(ran)(ran)(ran)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)在第(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)先(xian)各(ge)(ge)(ge)(ge)取50μl棄(qi)掉(diao)，再(zai)(zai)(zai)各(ge)(ge)(ge)(ge)取50μl分(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)在第(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50ul，混(hun)勻(yun)；混(hun)勻(yun)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)從第(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)(ge)(ge)取50μl分(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)八(ba)(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)在第(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)八(ba)(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)從第(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)八(ba)(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)取50μl加(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)九、第(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)在第(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)從第(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)(ge)(ge)取50μl棄(qi)掉(diao)。（稀(xi)釋(shi)(shi)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)各(ge)(ge)(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)都(dou)為(wei)50μl，濃(nong)(nong)度(du)(du)(du)(du)分(fen)(fen)(fen)別(bie)(bie)(bie)為(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)別(bie)(bie)(bie)設空(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)白(bai)(bai)對照孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)及酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，其(qi)余各(ge)(ge)(ge)(ge)步操作相(xiang)(xiang)同(tong)(tong)(tong)(tong)）、待(dai)(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)包(bao)(bao)被(bei)板(ban)(ban)上待(dai)(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)先(xian)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)40μl，然(ran)(ran)(ran)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)(zai)(zai)加(jia)(jia)(jia)(jia)待(dai)(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)＊終(zhong)稀(xi)釋(shi)(shi)度(du)(du)(du)(du)為(wei)5倍(bei)(bei)）。加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)于(yu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部(bu)，盡量(liang)不(bu)(bu)觸(chu)及孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁(bi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)晃動混(hun)勻(yun)。3. 溫(wen)(wen)育(yu)：用(yong)(yong)(yong)封(feng)(feng)(feng)(feng)板(ban)(ban)膜(mo)封(feng)(feng)(feng)(feng)板(ban)(ban)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)置(zhi)37℃溫(wen)(wen)育(yu)30分(fen)(fen)(fen)鐘(zhong)。4. 配液(ye)(ye)(ye)(ye)(ye)：將30（48T的(de)(de)(de)(de)(de)20倍(bei)(bei)）倍(bei)(bei)濃(nong)(nong)縮洗(xi)(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)用(yong)(yong)(yong)蒸餾水30（48T的(de)(de)(de)(de)(de)20倍(bei)(bei)）倍(bei)(bei)稀(xi)釋(shi)(shi)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)備(bei)用(yong)(yong)(yong)。5. 洗(xi)(xi)(xi)滌(di)：小(xiao)心揭掉(diao)封(feng)(feng)(feng)(feng)板(ban)(ban)膜(mo)，棄(qi)去液(ye)(ye)(ye)(ye)(ye)體(ti)，甩干，每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)，靜置(zhi)30秒后(hou)(hou)(hou)(hou)(hou)(hou)(hou)棄(qi)去，如此重(zhong)(zhong)復(fu)5次(ci)，拍干。6. 加(jia)(jia)(jia)(jia)酶(mei)(mei)(mei)(mei)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)50μl，空(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外(wai)。7. 溫(wen)(wen)育(yu)：操作同(tong)(tong)(tong)(tong)3。8. 洗(xi)(xi)(xi)滌(di)：操作同(tong)(tong)(tong)(tong)5。9. 顯(xian)色(se)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)(jia)(jia)入顯(xian)色(se)劑(ji)(ji)(ji)(ji)A50μl，再(zai)(zai)(zai)加(jia)(jia)(jia)(jia)入顯(xian)色(se)劑(ji)(ji)(ji)(ji)B50μl，輕(qing)(qing)(qing)輕(qing)(qing)(qing)震蕩混(hun)勻(yun)，37℃避(bi)光顯(xian)色(se)15分(fen)(fen)(fen)鐘(zhong). 10. 終(zhong)止：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止反應(ying)(ying)（此時(shi)(shi)藍色(se)立轉黃色(se)）。11. 測(ce)(ce)(ce)定(ding)：以(yi)空(kong)白(bai)(bai)空(kong)調(diao)零，450nm波長依(yi)序測(ce)(ce)(ce)量(liang)各(ge)(ge)(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)吸(xi)光度(du)(du)(du)(du)（OD值）。 測(ce)(ce)(ce)定(ding)應(ying)(ying)在加(jia)(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)鐘(zhong)以(yi)內(nei)進(jin)行(xing)。牛基(ji)(ji)質(zhi)金屬蛋白(bai)(bai)酶(mei)(mei)(mei)(mei)23B(MMP23B)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)【注意事項】1． 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)從冷藏(zang)環境(jing)(jing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)取出應(ying)(ying)在室溫(wen)(wen)平(ping)衡(heng)15-30分(fen)(fen)(fen)鐘(zhong)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)方可(ke)使(shi)(shi)用(yong)(yong)(yong)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)包(bao)(bao)被(bei)板(ban)(ban)開(kai)封(feng)(feng)(feng)(feng)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)如未(wei)用(yong)(yong)(yong)完，板(ban)(ban)條(tiao)應(ying)(ying)裝(zhuang)入密封(feng)(feng)(feng)(feng)袋中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。2． 濃(nong)(nong)洗(xi)(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)可(ke)能(neng)會有(you)(you)結(jie)(jie)晶(jing)析出，稀(xi)釋(shi)(shi)時(shi)(shi)可(ke)在水浴中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)溫(wen)(wen)助溶(rong)，洗(xi)(xi)(xi)滌(di)時(shi)(shi)不(bu)(bu)影(ying)響結(jie)(jie)果(guo)。3． 各(ge)(ge)(ge)(ge)步加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)均(jun)應(ying)(ying)使(shi)(shi)用(yong)(yong)(yong)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)器，并經常校對其(qi)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性，以(yi)避(bi)免(mian)試(shi)(shi)(shi)(shi)驗(yan)(yan)誤(wu)差。一次(ci)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)間(jian)＊好(hao)控制在5分(fen)(fen)(fen)鐘(zhong)內(nei)，如標(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)數(shu)(shu)(shu)量(liang)多(duo)，推(tui)薦使(shi)(shi)用(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4． 請(qing)(qing)每(mei)(mei)次(ci)測(ce)(ce)(ce)定(ding)的(de)(de)(de)(de)(de)同(tong)(tong)(tong)(tong)時(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)，＊好(hao)做(zuo)復(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如標(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)待(dai)(dai)測(ce)(ce)(ce)物(wu)質(zhi)含量(liang)過(guo)高（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)OD值大于(yu)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)一孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)OD值），請(qing)(qing)先(xian)用(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)釋(shi)(shi)一定(ding)倍(bei)(bei)數(shu)(shu)(shu)（n倍(bei)(bei)）后(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)(zai)(zai)測(ce)(ce)(ce)定(ding)，計算時(shi)(shi)請(qing)(qing)＊后(hou)(hou)(hou)(hou)(hou)(hou)(hou)乘以(yi)總稀(xi)釋(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)（×n×5）。5． 封(feng)(feng)(feng)(feng)板(ban)(ban)膜(mo)只(zhi)限一次(ci)性使(shi)(shi)用(yong)(yong)(yong)，以(yi)避(bi)免(mian)交(jiao)(jiao)叉(cha)污染。6． 底物(wu)請(qing)(qing)避(bi)光保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。7． 嚴格(ge)按(an)照說(shuo)明(ming)書(shu)(shu)的(de)(de)(de)(de)(de)操作進(jin)行(xing)，試(shi)(shi)(shi)(shi)驗(yan)(yan)結(jie)(jie)果(guo)判定(ding)必須以(yi)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)儀讀(du)數(shu)(shu)(shu)為(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun).8． 所有(you)(you)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)，洗(xi)(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)和各(ge)(ge)(ge)(ge)種廢棄(qi)物(wu)都(dou)應(ying)(ying)按(an)傳(chuan)染物(wu)處(chu)理。9． 本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)不(bu)(bu)同(tong)(tong)(tong)(tong)批號組(zu)(zu)分(fen)(fen)(fen)不(bu)(bu)得(de)(de)混(hun)用(yong)(yong)(yong)。10. 如與英(ying)文(wen)說(shuo)明(ming)書(shu)(shu)有(you)(you)異(yi)，以(yi)英(ying)文(wen)說(shuo)明(ming)書(shu)(shu)為(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)。【計算】 以(yi)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)的(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)為(wei)橫坐標(biao)(biao)(biao)(biao)(biao)，OD值為(wei)縱坐標(biao)(biao)(biao)(biao)(biao)， 在坐標(biao)(biao)(biao)(biao)(biao)紙上繪(hui)出標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)，根據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)OD 值由(you)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)查(cha)出相(xiang)(xiang)應(ying)(ying)的(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)；再(zai)(zai)(zai)乘以(yi)稀(xi)釋(shi)(shi) 倍(bei)(bei)數(shu)(shu)(shu)；或用(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)的(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)與OD值計算出標(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)的(de)(de)(de)(de)(de)直(zhi)線(xian)回(hui)歸方程式，將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)OD值 代(dai)入方程式，計算出樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)，再(zai)(zai)(zai)乘以(yi)稀(xi)釋(shi)(shi) 倍(bei)(bei)數(shu)(shu)(shu)，即為(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)實(shi)(shi)際濃(nong)(nong)度(du)(du)(du)(du)。 【試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)組(zu)(zu)成(cheng)】試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)組(zu)(zu)成(cheng)48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)說(shuo)明(ming)書(shu)(shu)1份(fen)1份(fen)密封(feng)(feng)(feng)(feng)袋1個(ge)1個(ge)封(feng)(feng)(feng)(feng)板(ban)(ban)膜(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)2片（96）2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)板(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)0.3mL*6管(guan)(guan)0.3mL*6管(guan)(guan)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)1.5ml×1瓶(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)6 ml×1瓶(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)6 ml×1瓶(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)顯(xian)色(se)劑(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)6 ml×1瓶(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)顯(xian)色(se)劑(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)6 ml×1瓶(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)終(zhong)止液(ye)(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)6ml×1瓶(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)注意：使(shi)(shi)用(yong)(yong)(yong)前(qian)請(qing)(qing)檢查(cha)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)簽和數(shu)(shu)(shu)量(liang)與表格(ge)是否一致(zhi)。【精(jing)(jing)密度(du)(du)(du)(du)】精(jing)(jing)密度(du)(du)(du)(du)用(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)測(ce)(ce)(ce)定(ding)值的(de)(de)(de)(de)(de)變(bian)異(yi)系數(shu)(shu)(shu)CV 表示。CV（%） = SD/mean×100批內(nei)差：取同(tong)(tong)(tong)(tong)批次(ci)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)對低、中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)、高值定(ding)值樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)進(jin)行(xing)定(ding)量(liang)檢測(ce)(ce)(ce)，每(mei)(mei)份(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)連續測(ce)(ce)(ce)定(ding)20 次(ci)，分(fen)(fen)(fen)別(bie)(bie)(bie)計算不(bu)(bu)同(tong)(tong)(tong)(tong)濃(nong)(nong)度(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)平(ping)均(jun)值及SD 值。批內(nei)差： CV<9%批間(jian)差：選(xuan)取3 個(ge)不(bu)(bu)同(tong)(tong)(tong)(tong)批次(ci)的(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)分(fen)(fen)(fen)別(bie)(bie)(bie)對低、中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)、高值定(ding)值樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)進(jin)行(xing)定(ding)量(liang)測(ce)(ce)(ce)定(ding)，每(mei)(mei)個(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)使(shi)(shi)用(yong)(yong)(yong)同(tong)(tong)(tong)(tong)一試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)重(zhong)(zhong)復(fu)測(ce)(ce)(ce)定(ding)10 次(ci)，分(fen)(fen)(fen)別(bie)(bie)(bie)計算不(bu)(bu)同(tong)(tong)(tong)(tong)濃(nong)(nong)度(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)平(ping)均(jun)值及SD 值。批間(jian)差： CV<11% 【特異(yi)性】本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)識(shi)別(bie)(bie)(bie)天然(ran)(ran)(ran)和重(zhong)(zhong)組(zu)(zu)微生(sheng)物(wu)硝(xiao)酸鹽還原酶(mei)(mei)(mei)(mei)（Nar），經檢測(ce)(ce)(ce)與其(qi)它(ta)相(xiang)(xiang)似物(wu)質(zhi)無明(ming)顯(xian)交(jiao)(jiao)叉(cha)反應(ying)(ying)。由(you)于(yu)受到(dao)技術及樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)來源(yuan)的(de)(de)(de)(de)(de)限制，不(bu)(bu)可(ke)能(neng)完成(cheng)所有(you)(you)相(xiang)(xiang)關(guan)或相(xiang)(xiang)似物(wu)質(zhi)交(jiao)(jiao)叉(cha)反應(ying)(ying)檢測(ce)(ce)(ce)，因(yin)此本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)有(you)(you)可(ke)能(neng)與未(wei)經檢測(ce)(ce)(ce)的(de)(de)(de)(de)(de)其(qi)它(ta)物(wu)質(zhi)有(you)(you)交(jiao)(jiao)叉(cha)反應(ying)(ying)。 【穩(wen)定(ding)性】試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)在有(you)(you)效期(qi)內(nei)務(wu)必按(an)推(tui)薦溫(wen)(wen)度(du)(du)(du)(du)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)，活性降(jiang)低率小(xiao)于(yu)5%。為(wei)減小(xiao)外(wai)部(bu)因(yin)素對試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)破壞前(qian)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)檢測(ce)(ce)(ce)值的(de)(de)(de)(de)(de)影(ying)響，實(shi)(shi)驗(yan)(yan)室的(de)(de)(de)(de)(de)環境(jing)(jing)條(tiao)件需盡量(liang)保(bao)(bao)(bao)(bao)持一致(zhi)，尤其(qi)是實(shi)(shi)驗(yan)(yan)室內(nei)溫(wen)(wen)度(du)(du)(du)(du)、濕度(du)(du)(du)(du)及溫(wen)(wen)育(yu)條(tiao)件。其(qi)次(ci)由(you)同(tong)(tong)(tong)(tong)一實(shi)(shi)驗(yan)(yan)員來進(jin)行(xing)操作可(ke)減少(shao)人為(wei)誤(wu)差。
牛(niu)硒(xi)(xi)蛋(dan)白(bai)(bai)1(SEP1)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)文(wen)(wen)(wen)名稱牛(niu)硒(xi)(xi)蛋(dan)白(bai)(bai)1(SEP1)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)規格(ge)96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)類(lei)型(xing)血清、血漿、細胞上清液(ye)(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)(ye)、體(ti)液(ye)(ye)(ye)(ye)(ye)、灌洗(xi)(xi)液(ye)(ye)(ye)(ye)(ye)等使用(yong)(yong)(yong)(yong)(yong)(yong)范圍科(ke)研實(shi)驗(yan)(yan)，不(bu)(bu)(bu)(bu)(bu)得用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)臨床診斷(duan)反(fan)應(ying)(ying)時(shi)間(jian)(jian)1-5h試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)保(bao)存(cun)(cun)(cun)2-8℃有(you)效(xiao)期(qi)6個(ge)月貨期(qi)3-5天(tian)【操(cao)作(zuo)步驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)與加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被(bei)板(ban)(ban)(ban)上設標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)第(di)(di)(di)(di)(di)(di)一(yi)(yi)(yi)(yi)(yi)、第(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，然后(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)(di)(di)一(yi)(yi)(yi)(yi)(yi)、第(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)(yun)(yun)；然后(hou)(hou)(hou)從(cong)(cong)第(di)(di)(di)(di)(di)(di)一(yi)(yi)(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)(qu)(qu)100μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)三(san)(san)孔(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)三(san)(san)、第(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)(yun)(yun)；然后(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)(di)(di)三(san)(san)孔(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)先(xian)(xian)各(ge)(ge)取(qu)(qu)(qu)50μl棄(qi)掉(diao)，再(zai)各(ge)(ge)取(qu)(qu)(qu)50μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50ul，混(hun)勻(yun)(yun)(yun)；混(hun)勻(yun)(yun)(yun)后(hou)(hou)(hou)從(cong)(cong)第(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)(qu)(qu)50μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)(di)八(ba)(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)(di)八(ba)(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)(yun)(yun)后(hou)(hou)(hou)從(cong)(cong)第(di)(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)(di)八(ba)(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)取(qu)(qu)(qu)50μl加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)九、第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)(yun)(yun)后(hou)(hou)(hou)從(cong)(cong)第(di)(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)(qu)(qu)50μl棄(qi)掉(diao)。（稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)(hou)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)都為(wei)(wei)(wei)(wei)50μl，濃(nong)(nong)度(du)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)為(wei)(wei)(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)設空(kong)(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)(kong)白(bai)(bai)對(dui)(dui)照孔(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)及(ji)(ji)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，其余各(ge)(ge)步操(cao)作(zuo)相(xiang)同(tong)(tong)(tong)(tong)）、待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被(bei)板(ban)(ban)(ban)上待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)先(xian)(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)40μl，然后(hou)(hou)(hou)再(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)＊終(zhong)(zhong)(zhong)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)度(du)為(wei)(wei)(wei)(wei)5倍(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)將樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)底部(bu)，盡量(liang)(liang)不(bu)(bu)(bu)(bu)(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)壁(bi)，輕(qing)(qing)輕(qing)(qing)晃(huang)動(dong)混(hun)勻(yun)(yun)(yun)。3. 溫(wen)(wen)育(yu)：用(yong)(yong)(yong)(yong)(yong)(yong)封(feng)(feng)(feng)板(ban)(ban)(ban)膜封(feng)(feng)(feng)板(ban)(ban)(ban)后(hou)(hou)(hou)置(zhi)37℃溫(wen)(wen)育(yu)30分(fen)(fen)(fen)鐘(zhong)(zhong)。4. 配液(ye)(ye)(ye)(ye)(ye)：將30（48T的(de)(de)(de)(de)20倍(bei)(bei)）倍(bei)(bei)濃(nong)(nong)縮洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)用(yong)(yong)(yong)(yong)(yong)(yong)蒸餾(liu)水30（48T的(de)(de)(de)(de)20倍(bei)(bei)）倍(bei)(bei)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)(hou)備用(yong)(yong)(yong)(yong)(yong)(yong)。5. 洗(xi)(xi)滌(di)(di)：小心揭掉(diao)封(feng)(feng)(feng)板(ban)(ban)(ban)膜，棄(qi)去液(ye)(ye)(ye)(ye)(ye)體(ti)，甩干，每孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)滿洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)，靜置(zhi)30秒(miao)后(hou)(hou)(hou)棄(qi)去，如(ru)此(ci)重復5次(ci)，拍干。6. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)(mei)：每孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)50μl，空(kong)(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)除外。7. 溫(wen)(wen)育(yu)：操(cao)作(zuo)同(tong)(tong)(tong)(tong)3。8. 洗(xi)(xi)滌(di)(di)：操(cao)作(zuo)同(tong)(tong)(tong)(tong)5。9. 顯(xian)(xian)色(se)(se)(se)：每孔(kong)(kong)(kong)(kong)(kong)(kong)先(xian)(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)顯(xian)(xian)色(se)(se)(se)劑(ji)(ji)(ji)(ji)A50μl，再(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)顯(xian)(xian)色(se)(se)(se)劑(ji)(ji)(ji)(ji)B50μl，輕(qing)(qing)輕(qing)(qing)震蕩混(hun)勻(yun)(yun)(yun)，37℃避(bi)光顯(xian)(xian)色(se)(se)(se)15分(fen)(fen)(fen)鐘(zhong)(zhong). 10. 終(zhong)(zhong)(zhong)止：每孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)(zhong)止反(fan)應(ying)(ying)（此(ci)時(shi)藍色(se)(se)(se)立(li)轉黃色(se)(se)(se)）。11. 測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)：以(yi)空(kong)(kong)白(bai)(bai)空(kong)(kong)調(diao)零，450nm波長(chang)依序測(ce)(ce)(ce)量(liang)(liang)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)吸(xi)光度(du)（OD值(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)應(ying)(ying)在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)(fen)鐘(zhong)(zhong)以(yi)內(nei)進行(xing)。牛(niu)硒(xi)(xi)蛋(dan)白(bai)(bai)1(SEP1)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)【注意事項】1． 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)從(cong)(cong)冷(leng)藏(zang)環境中(zhong)(zhong)(zhong)(zhong)取(qu)(qu)(qu)出(chu)應(ying)(ying)在(zai)(zai)室(shi)溫(wen)(wen)平衡15-30分(fen)(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)(hou)方可使用(yong)(yong)(yong)(yong)(yong)(yong)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被(bei)板(ban)(ban)(ban)開封(feng)(feng)(feng)后(hou)(hou)(hou)如(ru)未用(yong)(yong)(yong)(yong)(yong)(yong)完，板(ban)(ban)(ban)條應(ying)(ying)裝(zhuang)入(ru)(ru)(ru)密封(feng)(feng)(feng)袋中(zhong)(zhong)(zhong)(zhong)保(bao)存(cun)(cun)(cun)。2． 濃(nong)(nong)洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)可能會有(you)結(jie)晶析(xi)出(chu)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)時(shi)可在(zai)(zai)水浴中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)溫(wen)(wen)助溶(rong)，洗(xi)(xi)滌(di)(di)時(shi)不(bu)(bu)(bu)(bu)(bu)影響(xiang)結(jie)果。3． 各(ge)(ge)步加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)均(jun)(jun)應(ying)(ying)使用(yong)(yong)(yong)(yong)(yong)(yong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)器，并經(jing)常(chang)校對(dui)(dui)其準(zhun)(zhun)(zhun)(zhun)確性，以(yi)避(bi)免試(shi)(shi)(shi)(shi)驗(yan)(yan)誤差(cha)。一(yi)(yi)(yi)(yi)(yi)次(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)時(shi)間(jian)(jian)＊好控制(zhi)在(zai)(zai)5分(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)數(shu)量(liang)(liang)多，推薦使用(yong)(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)。4． 請(qing)(qing)(qing)每次(ci)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)同(tong)(tong)(tong)(tong)時(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲線，＊好做(zuo)復孔(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)中(zhong)(zhong)(zhong)(zhong)待測(ce)(ce)(ce)物(wu)(wu)質含量(liang)(liang)過高（樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)OD值(zhi)(zhi)(zhi)大于(yu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)一(yi)(yi)(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)），請(qing)(qing)(qing)先(xian)(xian)用(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)一(yi)(yi)(yi)(yi)(yi)定(ding)(ding)(ding)(ding)(ding)(ding)倍(bei)(bei)數(shu)（n倍(bei)(bei)）后(hou)(hou)(hou)再(zai)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)，計算(suan)(suan)時(shi)請(qing)(qing)(qing)＊后(hou)(hou)(hou)乘(cheng)以(yi)總稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)（×n×5）。5． 封(feng)(feng)(feng)板(ban)(ban)(ban)膜只(zhi)限一(yi)(yi)(yi)(yi)(yi)次(ci)性使用(yong)(yong)(yong)(yong)(yong)(yong)，以(yi)避(bi)免交(jiao)叉污染(ran)。6． 底物(wu)(wu)請(qing)(qing)(qing)避(bi)光保(bao)存(cun)(cun)(cun)。7． 嚴格(ge)按(an)照說(shuo)(shuo)明(ming)書的(de)(de)(de)(de)操(cao)作(zuo)進行(xing)，試(shi)(shi)(shi)(shi)驗(yan)(yan)結(jie)果判(pan)定(ding)(ding)(ding)(ding)(ding)(ding)必(bi)須以(yi)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀數(shu)為(wei)(wei)(wei)(wei)準(zhun)(zhun)(zhun)(zhun).8． 所(suo)有(you)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)，洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)和各(ge)(ge)種(zhong)廢棄(qi)物(wu)(wu)都應(ying)(ying)按(an)傳染(ran)物(wu)(wu)處理。9． 本(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)不(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)(tong)批(pi)(pi)(pi)號(hao)組分(fen)(fen)(fen)不(bu)(bu)(bu)(bu)(bu)得混(hun)用(yong)(yong)(yong)(yong)(yong)(yong)。10. 如(ru)與英(ying)(ying)文(wen)(wen)(wen)說(shuo)(shuo)明(ming)書有(you)異，以(yi)英(ying)(ying)文(wen)(wen)(wen)說(shuo)(shuo)明(ming)書為(wei)(wei)(wei)(wei)準(zhun)(zhun)(zhun)(zhun)。【計算(suan)(suan)】 以(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)濃(nong)(nong)度(du)為(wei)(wei)(wei)(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上繪(hui)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲線，根據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)OD 值(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲線查(cha)(cha)出(chu)相(xiang)應(ying)(ying)的(de)(de)(de)(de)濃(nong)(nong)度(du)；再(zai)乘(cheng)以(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)數(shu)；或用(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)濃(nong)(nong)度(du)與OD值(zhi)(zhi)(zhi)計算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)曲線的(de)(de)(de)(de)直(zhi)線回(hui)歸方程(cheng)式，將樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)OD值(zhi)(zhi)(zhi) 代入(ru)(ru)(ru)方程(cheng)式，計算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)，再(zai)乘(cheng)以(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)數(shu)，即為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)實(shi)際濃(nong)(nong)度(du)。 【試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)組成】試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)組成48孔(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)保(bao)存(cun)(cun)(cun)說(shuo)(shuo)明(ming)書1份(fen)1份(fen)密封(feng)(feng)(feng)袋1個(ge)1個(ge)封(feng)(feng)(feng)板(ban)(ban)(ban)膜12孔(kong)(kong)(kong)(kong)(kong)(kong)×4條2片（96）2-8℃保(bao)存(cun)(cun)(cun)微孔(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)×8條2-8℃保(bao)存(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)存(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)(ping)(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)顯(xian)(xian)色(se)(se)(se)劑(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)顯(xian)(xian)色(se)(se)(se)劑(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)終(zhong)(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)(ping)(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)注意：使用(yong)(yong)(yong)(yong)(yong)(yong)前請(qing)(qing)(qing)檢查(cha)(cha)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)的(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和數(shu)量(liang)(liang)與表格(ge)是否一(yi)(yi)(yi)(yi)(yi)致(zhi)。【精(jing)密度(du)】精(jing)密度(du)用(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)的(de)(de)(de)(de)變(bian)異系(xi)數(shu)CV 表示。CV（%） = SD/mean×100批(pi)(pi)(pi)內(nei)差(cha)：取(qu)(qu)(qu)同(tong)(tong)(tong)(tong)批(pi)(pi)(pi)次(ci)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)對(dui)(dui)低(di)(di)、中(zhong)(zhong)(zhong)(zhong)、高值(zhi)(zhi)(zhi)定(ding)(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)進行(xing)定(ding)(ding)(ding)(ding)(ding)(ding)量(liang)(liang)檢測(ce)(ce)(ce)，每份(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)連續(xu)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)20 次(ci)，分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)計算(suan)(suan)不(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)(tong)濃(nong)(nong)度(du)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)的(de)(de)(de)(de)平均(jun)(jun)值(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)。批(pi)(pi)(pi)內(nei)差(cha)： CV<9%批(pi)(pi)(pi)間(jian)(jian)差(cha)：選取(qu)(qu)(qu)3 個(ge)不(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)(tong)批(pi)(pi)(pi)次(ci)的(de)(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)對(dui)(dui)低(di)(di)、中(zhong)(zhong)(zhong)(zhong)、高值(zhi)(zhi)(zhi)定(ding)(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)進行(xing)定(ding)(ding)(ding)(ding)(ding)(ding)量(liang)(liang)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)，每個(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)使用(yong)(yong)(yong)(yong)(yong)(yong)同(tong)(tong)(tong)(tong)一(yi)(yi)(yi)(yi)(yi)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)重復測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)10 次(ci)，分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)計算(suan)(suan)不(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)(tong)濃(nong)(nong)度(du)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)的(de)(de)(de)(de)平均(jun)(jun)值(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)。批(pi)(pi)(pi)間(jian)(jian)差(cha)： CV<11% 【特異性】本(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)識別(bie)(bie)(bie)(bie)天(tian)然和重組微生物(wu)(wu)硝酸鹽還原酶(mei)(mei)(mei)（Nar），經(jing)檢測(ce)(ce)(ce)與其它(ta)相(xiang)似(si)物(wu)(wu)質無明(ming)顯(xian)(xian)交(jiao)叉反(fan)應(ying)(ying)。由(you)于(yu)受(shou)到技術及(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)來(lai)源(yuan)的(de)(de)(de)(de)限制(zhi)，不(bu)(bu)(bu)(bu)(bu)可能完成所(suo)有(you)相(xiang)關或相(xiang)似(si)物(wu)(wu)質交(jiao)叉反(fan)應(ying)(ying)檢測(ce)(ce)(ce)，因此(ci)本(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)有(you)可能與未經(jing)檢測(ce)(ce)(ce)的(de)(de)(de)(de)其它(ta)物(wu)(wu)質有(you)交(jiao)叉反(fan)應(ying)(ying)。 【穩定(ding)(ding)(ding)(ding)(ding)(ding)性】試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)在(zai)(zai)有(you)效(xiao)期(qi)內(nei)務必(bi)按(an)推薦溫(wen)(wen)度(du)保(bao)存(cun)(cun)(cun)，活(huo)性降低(di)(di)率小于(yu)5%。為(wei)(wei)(wei)(wei)減小外部(bu)因素對(dui)(dui)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)破壞前后(hou)(hou)(hou)檢測(ce)(ce)(ce)值(zhi)(zhi)(zhi)的(de)(de)(de)(de)影響(xiang)，實(shi)驗(yan)(yan)室(shi)的(de)(de)(de)(de)環境條件需盡量(liang)(liang)保(bao)持一(yi)(yi)(yi)(yi)(yi)致(zhi)，尤其是實(shi)驗(yan)(yan)室(shi)內(nei)溫(wen)(wen)度(du)、濕度(du)及(ji)(ji)溫(wen)(wen)育(yu)條件。其次(ci)由(you)同(tong)(tong)(tong)(tong)一(yi)(yi)(yi)(yi)(yi)實(shi)驗(yan)(yan)員(yuan)來(lai)進行(xing)操(cao)作(zuo)可減少人為(wei)(wei)(wei)(wei)誤差(cha)。
牛(niu)(niu)戊(wu)糖(tang)素(PTD)ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)文名稱牛(niu)(niu)戊(wu)糖(tang)素(PTD)ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)規格(ge)(ge)96T/48T樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)類型血(xue)清、血(xue)漿、細(xi)胞上(shang)清液(ye)(ye)(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)(ye)(ye)、體(ti)液(ye)(ye)(ye)(ye)(ye)(ye)、灌洗(xi)液(ye)(ye)(ye)(ye)(ye)(ye)等使(shi)(shi)用(yong)(yong)(yong)范圍科(ke)研(yan)實(shi)(shi)驗，不得用(yong)(yong)(yong)于臨床診斷反(fan)應(ying)(ying)(ying)(ying)時(shi)(shi)間(jian)1-5h試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)2-8℃有(you)(you)效期6個(ge)月貨期3-5天【操(cao)(cao)作(zuo)(zuo)步驟(zou)】1. 標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)的(de)(de)(de)稀(xi)(xi)釋(shi)(shi)與(yu)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)：在(zai)(zai)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板上(shang)設標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)第(di)(di)(di)(di)一(yi)(yi)(yi)、第(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)100μl，然(ran)(ran)后(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)一(yi)(yi)(yi)、第(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻；然(ran)(ran)后(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)一(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)各(ge)(ge)(ge)取100μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再在(zai)(zai)第(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻；然(ran)(ran)后(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)先(xian)各(ge)(ge)(ge)取50μl棄(qi)掉，再各(ge)(ge)(ge)取50μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)五、第(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，再在(zai)(zai)第(di)(di)(di)(di)五、第(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)50ul，混(hun)勻；混(hun)勻后(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)五、第(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)各(ge)(ge)(ge)取50μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)七、第(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，再在(zai)(zai)第(di)(di)(di)(di)七、第(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻后(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)七、第(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)取50μl加(jia)(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)九(jiu)、第(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，再在(zai)(zai)第(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻后(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)各(ge)(ge)(ge)取50μl棄(qi)掉。（稀(xi)(xi)釋(shi)(shi)后(hou)(hou)(hou)各(ge)(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)量(liang)(liang)都為(wei)(wei)50μl，濃(nong)(nong)度(du)(du)(du)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)為(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)設空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)白(bai)對(dui)照(zhao)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)及(ji)(ji)(ji)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，其余(yu)各(ge)(ge)(ge)步操(cao)(cao)作(zuo)(zuo)相(xiang)(xiang)同(tong)(tong)）、待(dai)(dai)(dai)測(ce)(ce)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板上(shang)待(dai)(dai)(dai)測(ce)(ce)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)40μl，然(ran)(ran)后(hou)(hou)(hou)再加(jia)(jia)(jia)(jia)(jia)(jia)待(dai)(dai)(dai)測(ce)(ce)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)＊終(zhong)稀(xi)(xi)釋(shi)(shi)度(du)(du)(du)為(wei)(wei)5倍(bei)(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)于酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)板孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底(di)部(bu)(bu)，盡(jin)量(liang)(liang)不觸(chu)及(ji)(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕輕晃動混(hun)勻。3. 溫育：用(yong)(yong)(yong)封(feng)(feng)板膜(mo)(mo)封(feng)(feng)板后(hou)(hou)(hou)置(zhi)(zhi)37℃溫育30分(fen)(fen)(fen)鐘。4. 配(pei)液(ye)(ye)(ye)(ye)(ye)(ye)：將(jiang)30（48T的(de)(de)(de)20倍(bei)(bei)(bei)）倍(bei)(bei)(bei)濃(nong)(nong)縮洗(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)用(yong)(yong)(yong)蒸餾水30（48T的(de)(de)(de)20倍(bei)(bei)(bei)）倍(bei)(bei)(bei)稀(xi)(xi)釋(shi)(shi)后(hou)(hou)(hou)備用(yong)(yong)(yong)。5. 洗(xi)滌(di)(di)：小(xiao)心(xin)揭掉封(feng)(feng)板膜(mo)(mo)，棄(qi)去(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，甩干(gan)，每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)滿洗(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)，靜置(zhi)(zhi)30秒(miao)后(hou)(hou)(hou)棄(qi)去(qu)，如(ru)(ru)此(ci)重復5次(ci)(ci)，拍干(gan)。6. 加(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)(mei)：每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)入酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)50μl，空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外。7. 溫育：操(cao)(cao)作(zuo)(zuo)同(tong)(tong)3。8. 洗(xi)滌(di)(di)：操(cao)(cao)作(zuo)(zuo)同(tong)(tong)5。9. 顯色(se)：每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)入顯色(se)劑(ji)(ji)(ji)(ji)(ji)A50μl，再加(jia)(jia)(jia)(jia)(jia)(jia)入顯色(se)劑(ji)(ji)(ji)(ji)(ji)B50μl，輕輕震蕩混(hun)勻，37℃避光(guang)(guang)顯色(se)15分(fen)(fen)(fen)鐘. 10. 終(zhong)止(zhi)：每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止(zhi)反(fan)應(ying)(ying)(ying)(ying)（此(ci)時(shi)(shi)藍色(se)立(li)轉(zhuan)黃(huang)色(se)）。11. 測(ce)(ce)定(ding)(ding)(ding)(ding)：以(yi)(yi)空(kong)白(bai)空(kong)調(diao)零，450nm波長依序測(ce)(ce)量(liang)(liang)各(ge)(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)吸(xi)光(guang)(guang)度(du)(du)(du)（OD值(zhi)(zhi)）。 測(ce)(ce)定(ding)(ding)(ding)(ding)應(ying)(ying)(ying)(ying)在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)(fen)鐘以(yi)(yi)內(nei)進行。牛(niu)(niu)戊(wu)糖(tang)素(PTD)ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)【注(zhu)意(yi)(yi)事項】1． 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)從(cong)冷藏環境(jing)中(zhong)(zhong)取出(chu)(chu)應(ying)(ying)(ying)(ying)在(zai)(zai)室(shi)(shi)溫平衡15-30分(fen)(fen)(fen)鐘后(hou)(hou)(hou)方(fang)(fang)可(ke)使(shi)(shi)用(yong)(yong)(yong)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板開(kai)封(feng)(feng)后(hou)(hou)(hou)如(ru)(ru)未(wei)用(yong)(yong)(yong)完，板條應(ying)(ying)(ying)(ying)裝入密封(feng)(feng)袋中(zhong)(zhong)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。2． 濃(nong)(nong)洗(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)可(ke)能會有(you)(you)結(jie)(jie)晶(jing)析出(chu)(chu)，稀(xi)(xi)釋(shi)(shi)時(shi)(shi)可(ke)在(zai)(zai)水浴中(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)溫助溶，洗(xi)滌(di)(di)時(shi)(shi)不影響結(jie)(jie)果。3． 各(ge)(ge)(ge)步加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)均(jun)應(ying)(ying)(ying)(ying)使(shi)(shi)用(yong)(yong)(yong)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)器，并經常校對(dui)其準(zhun)(zhun)(zhun)確性(xing)，以(yi)(yi)避免試(shi)(shi)(shi)(shi)(shi)驗誤差(cha)(cha)。一(yi)(yi)(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)時(shi)(shi)間(jian)＊好(hao)控制在(zai)(zai)5分(fen)(fen)(fen)鐘內(nei)，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)數(shu)(shu)量(liang)(liang)多，推薦使(shi)(shi)用(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)。4． 請(qing)每(mei)(mei)(mei)(mei)次(ci)(ci)測(ce)(ce)定(ding)(ding)(ding)(ding)的(de)(de)(de)同(tong)(tong)時(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)線(xian)，＊好(hao)做(zuo)復孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)中(zhong)(zhong)待(dai)(dai)(dai)測(ce)(ce)物(wu)質含(han)量(liang)(liang)過高（樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)OD值(zhi)(zhi)大于標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)一(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)OD值(zhi)(zhi)），請(qing)先(xian)用(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)釋(shi)(shi)一(yi)(yi)(yi)定(ding)(ding)(ding)(ding)倍(bei)(bei)(bei)數(shu)(shu)（n倍(bei)(bei)(bei)）后(hou)(hou)(hou)再測(ce)(ce)定(ding)(ding)(ding)(ding)，計(ji)算(suan)時(shi)(shi)請(qing)＊后(hou)(hou)(hou)乘(cheng)以(yi)(yi)總稀(xi)(xi)釋(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)（×n×5）。5． 封(feng)(feng)板膜(mo)(mo)只(zhi)限一(yi)(yi)(yi)次(ci)(ci)性(xing)使(shi)(shi)用(yong)(yong)(yong)，以(yi)(yi)避免交(jiao)叉污染(ran)。6． 底(di)物(wu)請(qing)避光(guang)(guang)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。7． 嚴(yan)格(ge)(ge)按(an)照(zhao)說(shuo)(shuo)明(ming)書的(de)(de)(de)操(cao)(cao)作(zuo)(zuo)進行，試(shi)(shi)(shi)(shi)(shi)驗結(jie)(jie)果判定(ding)(ding)(ding)(ding)必須以(yi)(yi)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)儀(yi)讀(du)數(shu)(shu)為(wei)(wei)準(zhun)(zhun)(zhun).8． 所(suo)有(you)(you)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)，洗(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)和各(ge)(ge)(ge)種(zhong)廢棄(qi)物(wu)都應(ying)(ying)(ying)(ying)按(an)傳染(ran)物(wu)處理。9． 本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)不同(tong)(tong)批號(hao)組分(fen)(fen)(fen)不得混(hun)用(yong)(yong)(yong)。10. 如(ru)(ru)與(yu)英文說(shuo)(shuo)明(ming)書有(you)(you)異，以(yi)(yi)英文說(shuo)(shuo)明(ming)書為(wei)(wei)準(zhun)(zhun)(zhun)。【計(ji)算(suan)】 以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)的(de)(de)(de)濃(nong)(nong)度(du)(du)(du)為(wei)(wei)橫(heng)坐(zuo)標(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)為(wei)(wei)縱(zong)坐(zuo)標(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)紙上(shang)繪出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)線(xian)，根據樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)OD 值(zhi)(zhi)由(you)(you)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)線(xian)查出(chu)(chu)相(xiang)(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)濃(nong)(nong)度(du)(du)(du)；再乘(cheng)以(yi)(yi)稀(xi)(xi)釋(shi)(shi) 倍(bei)(bei)(bei)數(shu)(shu)；或用(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)的(de)(de)(de)濃(nong)(nong)度(du)(du)(du)與(yu)OD值(zhi)(zhi)計(ji)算(suan)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)曲(qu)線(xian)的(de)(de)(de)直線(xian)回(hui)歸方(fang)(fang)程(cheng)式，將(jiang)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)OD值(zhi)(zhi) 代(dai)入方(fang)(fang)程(cheng)式，計(ji)算(suan)出(chu)(chu)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)，再乘(cheng)以(yi)(yi)稀(xi)(xi)釋(shi)(shi) 倍(bei)(bei)(bei)數(shu)(shu)，即為(wei)(wei)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)實(shi)(shi)際濃(nong)(nong)度(du)(du)(du)。 【試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)組成】試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)組成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)(zhi)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)說(shuo)(shuo)明(ming)書1份(fen)1份(fen)密封(feng)(feng)袋1個(ge)1個(ge)封(feng)(feng)板膜(mo)(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條2片（96）2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)板1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)顯色(se)劑(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)顯色(se)劑(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)終(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)注(zhu)意(yi)(yi)：使(shi)(shi)用(yong)(yong)(yong)前請(qing)檢(jian)(jian)查試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)簽和數(shu)(shu)量(liang)(liang)與(yu)表格(ge)(ge)是否一(yi)(yi)(yi)致。【精密度(du)(du)(du)】精密度(du)(du)(du)用(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)測(ce)(ce)定(ding)(ding)(ding)(ding)值(zhi)(zhi)的(de)(de)(de)變異系(xi)數(shu)(shu)CV 表示。CV（%） = SD/mean×100批內(nei)差(cha)(cha)：取同(tong)(tong)批次(ci)(ci)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)對(dui)低(di)、中(zhong)(zhong)、高值(zhi)(zhi)定(ding)(ding)(ding)(ding)值(zhi)(zhi)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)進行定(ding)(ding)(ding)(ding)量(liang)(liang)檢(jian)(jian)測(ce)(ce)，每(mei)(mei)(mei)(mei)份(fen)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)連續測(ce)(ce)定(ding)(ding)(ding)(ding)20 次(ci)(ci)，分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)計(ji)算(suan)不同(tong)(tong)濃(nong)(nong)度(du)(du)(du)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)平均(jun)值(zhi)(zhi)及(ji)(ji)(ji)SD 值(zhi)(zhi)。批內(nei)差(cha)(cha)： CV<9%批間(jian)差(cha)(cha)：選取3 個(ge)不同(tong)(tong)批次(ci)(ci)的(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)對(dui)低(di)、中(zhong)(zhong)、高值(zhi)(zhi)定(ding)(ding)(ding)(ding)值(zhi)(zhi)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)進行定(ding)(ding)(ding)(ding)量(liang)(liang)測(ce)(ce)定(ding)(ding)(ding)(ding)，每(mei)(mei)(mei)(mei)個(ge)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)使(shi)(shi)用(yong)(yong)(yong)同(tong)(tong)一(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)重復測(ce)(ce)定(ding)(ding)(ding)(ding)10 次(ci)(ci)，分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)計(ji)算(suan)不同(tong)(tong)濃(nong)(nong)度(du)(du)(du)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)平均(jun)值(zhi)(zhi)及(ji)(ji)(ji)SD 值(zhi)(zhi)。批間(jian)差(cha)(cha)： CV<11% 【特異性(xing)】本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)識別(bie)(bie)(bie)(bie)(bie)(bie)天然(ran)(ran)和重組微生物(wu)硝酸鹽還原酶(mei)(mei)(mei)（Nar），經檢(jian)(jian)測(ce)(ce)與(yu)其它相(xiang)(xiang)似物(wu)質無明(ming)顯交(jiao)叉反(fan)應(ying)(ying)(ying)(ying)。由(you)(you)于受到技(ji)術及(ji)(ji)(ji)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)來(lai)源的(de)(de)(de)限制，不可(ke)能完成所(suo)有(you)(you)相(xiang)(xiang)關或相(xiang)(xiang)似物(wu)質交(jiao)叉反(fan)應(ying)(ying)(ying)(ying)檢(jian)(jian)測(ce)(ce)，因(yin)此(ci)本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)有(you)(you)可(ke)能與(yu)未(wei)經檢(jian)(jian)測(ce)(ce)的(de)(de)(de)其它物(wu)質有(you)(you)交(jiao)叉反(fan)應(ying)(ying)(ying)(ying)。 【穩定(ding)(ding)(ding)(ding)性(xing)】試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)在(zai)(zai)有(you)(you)效期內(nei)務必按(an)推薦溫度(du)(du)(du)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)，活性(xing)降低(di)率小(xiao)于5%。為(wei)(wei)減小(xiao)外部(bu)(bu)因(yin)素對(dui)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)破壞前后(hou)(hou)(hou)檢(jian)(jian)測(ce)(ce)值(zhi)(zhi)的(de)(de)(de)影響，實(shi)(shi)驗室(shi)(shi)的(de)(de)(de)環境(jing)條件(jian)需盡(jin)量(liang)(liang)保(bao)(bao)(bao)(bao)(bao)持一(yi)(yi)(yi)致，尤(you)其是實(shi)(shi)驗室(shi)(shi)內(nei)溫度(du)(du)(du)、濕度(du)(du)(du)及(ji)(ji)(ji)溫育條件(jian)。其次(ci)(ci)由(you)(you)同(tong)(tong)一(yi)(yi)(yi)實(shi)(shi)驗員來(lai)進行操(cao)(cao)作(zuo)(zuo)可(ke)減少人為(wei)(wei)誤差(cha)(cha)。
牛(niu)乳脂(zhi)球表(biao)(biao)皮生(sheng)長因(yin)(yin)子(zi)(zi)8(MFGE8)ELISA 試(shi)(shi)劑(ji)盒(he)(he)(he)(he)中(zhong)(zhong)(zhong)文名稱牛(niu)乳脂(zhi)球表(biao)(biao)皮生(sheng)長因(yin)(yin)子(zi)(zi)8(MFGE8)ELISA 試(shi)(shi)劑(ji)盒(he)(he)(he)(he)規格96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)類型血清(qing)、血漿(jiang)、細胞上(shang)清(qing)液(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)、體液(ye)(ye)(ye)(ye)、灌(guan)洗(xi)液(ye)(ye)(ye)(ye)等使用(yong)(yong)范圍科研實驗，不(bu)(bu)得用(yong)(yong)于(yu)臨(lin)床診斷反(fan)(fan)應(ying)(ying)(ying)時(shi)(shi)間1-5h試(shi)(shi)劑(ji)盒(he)(he)(he)(he)保(bao)存(cun)(cun)2-8℃有(you)(you)效期6個月貨期3-5天(tian)【操(cao)作(zuo)(zuo)(zuo)(zuo)步驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)與(yu)(yu)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)上(shang)設(she)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)100μl，然(ran)(ran)后(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)(yun)；然(ran)(ran)后(hou)(hou)(hou)從第(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)各(ge)(ge)(ge)取(qu)(qu)(qu)100μl分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)(yun)；然(ran)(ran)后(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)先各(ge)(ge)(ge)取(qu)(qu)(qu)50μl棄(qi)(qi)掉(diao)(diao)，再(zai)(zai)各(ge)(ge)(ge)取(qu)(qu)(qu)50μl分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)50ul，混(hun)(hun)(hun)勻(yun)(yun)；混(hun)(hun)(hun)勻(yun)(yun)后(hou)(hou)(hou)從第(di)(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)各(ge)(ge)(ge)取(qu)(qu)(qu)50μl分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)(yun)后(hou)(hou)(hou)從第(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)取(qu)(qu)(qu)50μl加(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)九(jiu)、第(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)(yun)后(hou)(hou)(hou)從第(di)(di)(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)各(ge)(ge)(ge)取(qu)(qu)(qu)50μl棄(qi)(qi)掉(diao)(diao)。（稀(xi)(xi)(xi)釋(shi)(shi)后(hou)(hou)(hou)各(ge)(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)(liang)都為(wei)(wei)50μl，濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)為(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)設(she)空(kong)(kong)(kong)(kong)(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)(kong)(kong)(kong)(kong)白對(dui)照孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)及(ji)(ji)(ji)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)劑(ji)，其(qi)(qi)余各(ge)(ge)(ge)步操(cao)作(zuo)(zuo)(zuo)(zuo)相同(tong)）、待(dai)(dai)測樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)上(shang)待(dai)(dai)測樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)先加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)40μl，然(ran)(ran)后(hou)(hou)(hou)再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)待(dai)(dai)測樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)＊終(zhong)稀(xi)(xi)(xi)釋(shi)(shi)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)5倍(bei)(bei)(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)于(yu)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部(bu)，盡量(liang)(liang)(liang)不(bu)(bu)觸及(ji)(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕輕晃動(dong)混(hun)(hun)(hun)勻(yun)(yun)。3. 溫(wen)育(yu)：用(yong)(yong)封(feng)(feng)(feng)板(ban)膜(mo)封(feng)(feng)(feng)板(ban)后(hou)(hou)(hou)置(zhi)37℃溫(wen)育(yu)30分(fen)(fen)(fen)(fen)(fen)(fen)鐘。4. 配液(ye)(ye)(ye)(ye)：將30（48T的(de)(de)(de)(de)20倍(bei)(bei)(bei)(bei)）倍(bei)(bei)(bei)(bei)濃(nong)(nong)(nong)(nong)縮洗(xi)滌(di)(di)液(ye)(ye)(ye)(ye)用(yong)(yong)蒸餾水30（48T的(de)(de)(de)(de)20倍(bei)(bei)(bei)(bei)）倍(bei)(bei)(bei)(bei)稀(xi)(xi)(xi)釋(shi)(shi)后(hou)(hou)(hou)備用(yong)(yong)。5. 洗(xi)滌(di)(di)：小(xiao)心(xin)揭掉(diao)(diao)封(feng)(feng)(feng)板(ban)膜(mo)，棄(qi)(qi)去(qu)液(ye)(ye)(ye)(ye)體，甩干，每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)滿洗(xi)滌(di)(di)液(ye)(ye)(ye)(ye)，靜置(zhi)30秒后(hou)(hou)(hou)棄(qi)(qi)去(qu)，如(ru)此(ci)重(zhong)復(fu)5次(ci)(ci)(ci)(ci)，拍干。6. 加(jia)(jia)(jia)(jia)(jia)酶：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)劑(ji)50μl，空(kong)(kong)(kong)(kong)(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除(chu)外。7. 溫(wen)育(yu)：操(cao)作(zuo)(zuo)(zuo)(zuo)同(tong)3。8. 洗(xi)滌(di)(di)：操(cao)作(zuo)(zuo)(zuo)(zuo)同(tong)5。9. 顯(xian)色(se)：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)顯(xian)色(se)劑(ji)A50μl，再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)顯(xian)色(se)劑(ji)B50μl，輕輕震蕩混(hun)(hun)(hun)勻(yun)(yun)，37℃避光顯(xian)色(se)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘. 10. 終(zhong)止(zhi)：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)液(ye)(ye)(ye)(ye)50μl，終(zhong)止(zhi)反(fan)(fan)應(ying)(ying)(ying)（此(ci)時(shi)(shi)藍色(se)立轉黃(huang)色(se)）。11. 測定(ding)(ding)(ding)(ding)：以空(kong)(kong)(kong)(kong)(kong)白空(kong)(kong)(kong)(kong)(kong)調零(ling)，450nm波長依序測量(liang)(liang)(liang)各(ge)(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)吸光度(du)(du)(du)(du)(du)(du)(du)（OD值(zhi)(zhi)）。 測定(ding)(ding)(ding)(ding)應(ying)(ying)(ying)在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)液(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘以內(nei)進(jin)行。牛(niu)乳脂(zhi)球表(biao)(biao)皮生(sheng)長因(yin)(yin)子(zi)(zi)8(MFGE8)ELISA 試(shi)(shi)劑(ji)盒(he)(he)(he)(he)【注意事項(xiang)】1． 試(shi)(shi)劑(ji)盒(he)(he)(he)(he)從冷藏(zang)環(huan)境中(zhong)(zhong)(zhong)取(qu)(qu)(qu)出應(ying)(ying)(ying)在(zai)(zai)室溫(wen)平衡15-30分(fen)(fen)(fen)(fen)(fen)(fen)鐘后(hou)(hou)(hou)方可(ke)使用(yong)(yong)，酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)開封(feng)(feng)(feng)后(hou)(hou)(hou)如(ru)未用(yong)(yong)完，板(ban)條應(ying)(ying)(ying)裝入(ru)(ru)(ru)密(mi)(mi)封(feng)(feng)(feng)袋(dai)中(zhong)(zhong)(zhong)保(bao)存(cun)(cun)。2． 濃(nong)(nong)(nong)(nong)洗(xi)滌(di)(di)液(ye)(ye)(ye)(ye)可(ke)能會有(you)(you)結(jie)晶(jing)析出，稀(xi)(xi)(xi)釋(shi)(shi)時(shi)(shi)可(ke)在(zai)(zai)水浴中(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)溫(wen)助溶，洗(xi)滌(di)(di)時(shi)(shi)不(bu)(bu)影響結(jie)果。3． 各(ge)(ge)(ge)步加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)均(jun)應(ying)(ying)(ying)使用(yong)(yong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)器，并經(jing)常校對(dui)其(qi)(qi)準(zhun)(zhun)確性(xing)(xing)(xing)，以避免試(shi)(shi)驗誤差(cha)。一(yi)(yi)次(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)間＊好控(kong)制(zhi)在(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)鐘內(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)數(shu)量(liang)(liang)(liang)多，推(tui)薦使用(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4． 請(qing)(qing)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)測定(ding)(ding)(ding)(ding)的(de)(de)(de)(de)同(tong)時(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲線(xian)，＊好做復(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)中(zhong)(zhong)(zhong)待(dai)(dai)測物(wu)(wu)質含量(liang)(liang)(liang)過高（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)OD值(zhi)(zhi)大于(yu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)OD值(zhi)(zhi)），請(qing)(qing)先用(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)釋(shi)(shi)一(yi)(yi)定(ding)(ding)(ding)(ding)倍(bei)(bei)(bei)(bei)數(shu)（n倍(bei)(bei)(bei)(bei)）后(hou)(hou)(hou)再(zai)(zai)測定(ding)(ding)(ding)(ding)，計(ji)(ji)(ji)(ji)算(suan)時(shi)(shi)請(qing)(qing)＊后(hou)(hou)(hou)乘以總稀(xi)(xi)(xi)釋(shi)(shi)倍(bei)(bei)(bei)(bei)數(shu)（×n×5）。5． 封(feng)(feng)(feng)板(ban)膜(mo)只(zhi)限一(yi)(yi)次(ci)(ci)(ci)(ci)性(xing)(xing)(xing)使用(yong)(yong)，以避免交叉(cha)污染。6． 底物(wu)(wu)請(qing)(qing)避光保(bao)存(cun)(cun)。7． 嚴格按(an)照說(shuo)明書(shu)的(de)(de)(de)(de)操(cao)作(zuo)(zuo)(zuo)(zuo)進(jin)行，試(shi)(shi)驗結(jie)果判定(ding)(ding)(ding)(ding)必須以酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀數(shu)為(wei)(wei)準(zhun)(zhun).8． 所(suo)有(you)(you)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)，洗(xi)滌(di)(di)液(ye)(ye)(ye)(ye)和各(ge)(ge)(ge)種(zhong)廢棄(qi)(qi)物(wu)(wu)都應(ying)(ying)(ying)按(an)傳染物(wu)(wu)處(chu)理。9． 本(ben)(ben)(ben)試(shi)(shi)劑(ji)不(bu)(bu)同(tong)批(pi)號組分(fen)(fen)(fen)(fen)(fen)(fen)不(bu)(bu)得混(hun)(hun)(hun)用(yong)(yong)。10. 如(ru)與(yu)(yu)英文說(shuo)明書(shu)有(you)(you)異，以英文說(shuo)明書(shu)為(wei)(wei)準(zhun)(zhun)。【計(ji)(ji)(ji)(ji)算(suan)】 以標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)橫(heng)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)為(wei)(wei)縱(zong)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)繪出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲線(xian)，根據樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)OD 值(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲線(xian)查(cha)出相應(ying)(ying)(ying)的(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)；再(zai)(zai)乘以稀(xi)(xi)(xi)釋(shi)(shi) 倍(bei)(bei)(bei)(bei)數(shu)；或(huo)用(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)與(yu)(yu)OD值(zhi)(zhi)計(ji)(ji)(ji)(ji)算(suan)出標(biao)(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)曲線(xian)的(de)(de)(de)(de)直線(xian)回歸方程式，將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)OD值(zhi)(zhi) 代入(ru)(ru)(ru)方程式，計(ji)(ji)(ji)(ji)算(suan)出樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)，再(zai)(zai)乘以稀(xi)(xi)(xi)釋(shi)(shi) 倍(bei)(bei)(bei)(bei)數(shu)，即為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)實際(ji)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。 【試(shi)(shi)劑(ji)盒(he)(he)(he)(he)組成(cheng)】試(shi)(shi)劑(ji)盒(he)(he)(he)(he)組成(cheng)48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)保(bao)存(cun)(cun)說(shuo)明書(shu)1份1份密(mi)(mi)封(feng)(feng)(feng)袋(dai)1個1個封(feng)(feng)(feng)板(ban)膜(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條2片(pian)（96）2-8℃保(bao)存(cun)(cun)微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條2-8℃保(bao)存(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)0.3mL*6管(guan)0.3mL*6管(guan)2-8℃保(bao)存(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)劑(ji)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)顯(xian)色(se)劑(ji)A液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)顯(xian)色(se)劑(ji)B液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)終(zhong)止(zhi)液(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)注意：使用(yong)(yong)前(qian)請(qing)(qing)檢(jian)查(cha)試(shi)(shi)劑(ji)盒(he)(he)(he)(he)中(zhong)(zhong)(zhong)試(shi)(shi)劑(ji)的(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和數(shu)量(liang)(liang)(liang)與(yu)(yu)表(biao)(biao)格是(shi)否一(yi)(yi)致(zhi)。【精(jing)密(mi)(mi)度(du)(du)(du)(du)(du)(du)(du)】精(jing)密(mi)(mi)度(du)(du)(du)(du)(du)(du)(du)用(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)測定(ding)(ding)(ding)(ding)值(zhi)(zhi)的(de)(de)(de)(de)變異系數(shu)CV 表(biao)(biao)示(shi)。CV（%） = SD/mean×100批(pi)內(nei)差(cha)：取(qu)(qu)(qu)同(tong)批(pi)次(ci)(ci)(ci)(ci)試(shi)(shi)劑(ji)盒(he)(he)(he)(he)對(dui)低(di)、中(zhong)(zhong)(zhong)、高值(zhi)(zhi)定(ding)(ding)(ding)(ding)值(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)進(jin)行定(ding)(ding)(ding)(ding)量(liang)(liang)(liang)檢(jian)測，每(mei)(mei)(mei)份樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)連續測定(ding)(ding)(ding)(ding)20 次(ci)(ci)(ci)(ci)，分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)計(ji)(ji)(ji)(ji)算(suan)不(bu)(bu)同(tong)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)平均(jun)值(zhi)(zhi)及(ji)(ji)(ji)SD 值(zhi)(zhi)。批(pi)內(nei)差(cha)： CV<9%批(pi)間差(cha)：選取(qu)(qu)(qu)3 個不(bu)(bu)同(tong)批(pi)次(ci)(ci)(ci)(ci)的(de)(de)(de)(de)試(shi)(shi)劑(ji)盒(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)對(dui)低(di)、中(zhong)(zhong)(zhong)、高值(zhi)(zhi)定(ding)(ding)(ding)(ding)值(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)進(jin)行定(ding)(ding)(ding)(ding)量(liang)(liang)(liang)測定(ding)(ding)(ding)(ding)，每(mei)(mei)(mei)個樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)使用(yong)(yong)同(tong)一(yi)(yi)試(shi)(shi)劑(ji)盒(he)(he)(he)(he)重(zhong)復(fu)測定(ding)(ding)(ding)(ding)10 次(ci)(ci)(ci)(ci)，分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)計(ji)(ji)(ji)(ji)算(suan)不(bu)(bu)同(tong)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)平均(jun)值(zhi)(zhi)及(ji)(ji)(ji)SD 值(zhi)(zhi)。批(pi)間差(cha)： CV<11% 【特異性(xing)(xing)(xing)】本(ben)(ben)(ben)試(shi)(shi)劑(ji)盒(he)(he)(he)(he)識別(bie)(bie)(bie)(bie)天(tian)然(ran)(ran)和重(zhong)組微(wei)生(sheng)物(wu)(wu)硝酸鹽還(huan)原酶（Nar），經(jing)檢(jian)測與(yu)(yu)其(qi)(qi)它(ta)相似(si)物(wu)(wu)質無明顯(xian)交叉(cha)反(fan)(fan)應(ying)(ying)(ying)。由(you)于(yu)受到(dao)技術及(ji)(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)來源的(de)(de)(de)(de)限制(zhi)，不(bu)(bu)可(ke)能完成(cheng)所(suo)有(you)(you)相關或(huo)相似(si)物(wu)(wu)質交叉(cha)反(fan)(fan)應(ying)(ying)(ying)檢(jian)測，因(yin)(yin)此(ci)本(ben)(ben)(ben)試(shi)(shi)劑(ji)盒(he)(he)(he)(he)有(you)(you)可(ke)能與(yu)(yu)未經(jing)檢(jian)測的(de)(de)(de)(de)其(qi)(qi)它(ta)物(wu)(wu)質有(you)(you)交叉(cha)反(fan)(fan)應(ying)(ying)(ying)。 【穩定(ding)(ding)(ding)(ding)性(xing)(xing)(xing)】試(shi)(shi)劑(ji)盒(he)(he)(he)(he)在(zai)(zai)有(you)(you)效期內(nei)務必按(an)推(tui)薦溫(wen)度(du)(du)(du)(du)(du)(du)(du)保(bao)存(cun)(cun)，活性(xing)(xing)(xing)降低(di)率小(xiao)于(yu)5%。為(wei)(wei)減小(xiao)外部(bu)因(yin)(yin)素(su)對(dui)試(shi)(shi)劑(ji)盒(he)(he)(he)(he)破壞(huai)前(qian)后(hou)(hou)(hou)檢(jian)測值(zhi)(zhi)的(de)(de)(de)(de)影響，實驗室的(de)(de)(de)(de)環(huan)境條件需盡量(liang)(liang)(liang)保(bao)持一(yi)(yi)致(zhi)，尤其(qi)(qi)是(shi)實驗室內(nei)溫(wen)度(du)(du)(du)(du)(du)(du)(du)、濕(shi)度(du)(du)(du)(du)(du)(du)(du)及(ji)(ji)(ji)溫(wen)育(yu)條件。其(qi)(qi)次(ci)(ci)(ci)(ci)由(you)同(tong)一(yi)(yi)實驗員來進(jin)行操(cao)作(zuo)(zuo)(zuo)(zuo)可(ke)減少人(ren)為(wei)(wei)誤差(cha)。
牛胃(wei)(wei)粘液(ye)(ye)(ye)(ye)(ye)(ye)素(GM)ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)文名稱牛胃(wei)(wei)粘液(ye)(ye)(ye)(ye)(ye)(ye)素(GM)ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)規格96T/48T樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)類(lei)型(xing)血清(qing)、血漿、細(xi)胞(bao)上(shang)清(qing)液(ye)(ye)(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)(ye)(ye)、體液(ye)(ye)(ye)(ye)(ye)(ye)、灌(guan)洗(xi)(xi)液(ye)(ye)(ye)(ye)(ye)(ye)等使(shi)用(yong)(yong)(yong)(yong)范(fan)圍(wei)科研實驗(yan)，不(bu)(bu)(bu)(bu)得(de)用(yong)(yong)(yong)(yong)于(yu)(yu)臨床診斷反(fan)應(ying)(ying)時(shi)(shi)(shi)(shi)(shi)間(jian)1-5h試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)保(bao)(bao)(bao)存(cun)(cun)(cun)2-8℃有(you)效(xiao)期6個月貨(huo)期3-5天【操作步(bu)驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)與加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)(zai)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)上(shang)設標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)(zai)第(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，然后(hou)(hou)(hou)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)(yun)；然后(hou)(hou)(hou)從(cong)(cong)第(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)各(ge)(ge)取(qu)(qu)100μl分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)第(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)(yun)；然后(hou)(hou)(hou)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)第(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)先各(ge)(ge)取(qu)(qu)50μl棄掉(diao)，再(zai)(zai)(zai)各(ge)(ge)取(qu)(qu)50μl分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，再(zai)(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)50ul，混(hun)勻(yun)(yun)；混(hun)勻(yun)(yun)后(hou)(hou)(hou)從(cong)(cong)第(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)各(ge)(ge)取(qu)(qu)50μl分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，再(zai)(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)(yun)后(hou)(hou)(hou)從(cong)(cong)第(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)取(qu)(qu)50μl加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)九、第(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，再(zai)(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)(yun)后(hou)(hou)(hou)從(cong)(cong)第(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)各(ge)(ge)取(qu)(qu)50μl棄掉(diao)。（稀(xi)(xi)(xi)釋(shi)(shi)(shi)后(hou)(hou)(hou)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)量(liang)(liang)都(dou)為(wei)(wei)50μl，濃(nong)(nong)(nong)度(du)(du)(du)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)為(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)設空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)白(bai)對(dui)照孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)及(ji)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，其(qi)余各(ge)(ge)步(bu)操作相同）、待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)(zai)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)上(shang)待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)先加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)40μl，然后(hou)(hou)(hou)再(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)＊終稀(xi)(xi)(xi)釋(shi)(shi)(shi)度(du)(du)(du)為(wei)(wei)5倍(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)將樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)(yu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)板(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部，盡(jin)量(liang)(liang)不(bu)(bu)(bu)(bu)觸及(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕輕晃(huang)動(dong)混(hun)勻(yun)(yun)。3. 溫(wen)育：用(yong)(yong)(yong)(yong)封(feng)(feng)板(ban)膜(mo)封(feng)(feng)板(ban)后(hou)(hou)(hou)置(zhi)37℃溫(wen)育30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)。4. 配液(ye)(ye)(ye)(ye)(ye)(ye)：將30（48T的(de)(de)20倍(bei)(bei)）倍(bei)(bei)濃(nong)(nong)(nong)縮洗(xi)(xi)滌液(ye)(ye)(ye)(ye)(ye)(ye)用(yong)(yong)(yong)(yong)蒸餾水30（48T的(de)(de)20倍(bei)(bei)）倍(bei)(bei)稀(xi)(xi)(xi)釋(shi)(shi)(shi)后(hou)(hou)(hou)備用(yong)(yong)(yong)(yong)。5. 洗(xi)(xi)滌：小(xiao)心揭掉(diao)封(feng)(feng)板(ban)膜(mo)，棄去液(ye)(ye)(ye)(ye)(ye)(ye)體，甩(shuai)干，每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)滿洗(xi)(xi)滌液(ye)(ye)(ye)(ye)(ye)(ye)，靜置(zhi)30秒后(hou)(hou)(hou)棄去，如此(ci)重(zhong)(zhong)復5次(ci)(ci)，拍干。6. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)(mei)：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)50μl，空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外。7. 溫(wen)育：操作同3。8. 洗(xi)(xi)滌：操作同5。9. 顯色(se)(se)：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入顯色(se)(se)劑(ji)(ji)(ji)(ji)(ji)(ji)A50μl，再(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入顯色(se)(se)劑(ji)(ji)(ji)(ji)(ji)(ji)B50μl，輕輕震蕩混(hun)勻(yun)(yun)，37℃避(bi)光顯色(se)(se)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong). 10. 終止(zhi)：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，終止(zhi)反(fan)應(ying)(ying)（此(ci)時(shi)(shi)(shi)(shi)(shi)藍色(se)(se)立轉黃色(se)(se)）。11. 測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)：以(yi)空(kong)白(bai)空(kong)調(diao)零(ling)，450nm波長依(yi)序測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)吸光度(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)應(ying)(ying)在(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)以(yi)內(nei)進(jin)(jin)行。牛胃(wei)(wei)粘液(ye)(ye)(ye)(ye)(ye)(ye)素(GM)ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)【注(zhu)意(yi)事項】1． 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)從(cong)(cong)冷藏環(huan)境中(zhong)(zhong)取(qu)(qu)出(chu)(chu)應(ying)(ying)在(zai)(zai)(zai)室(shi)溫(wen)平衡15-30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)后(hou)(hou)(hou)方(fang)可(ke)(ke)(ke)(ke)使(shi)用(yong)(yong)(yong)(yong)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)開封(feng)(feng)后(hou)(hou)(hou)如未用(yong)(yong)(yong)(yong)完，板(ban)條(tiao)應(ying)(ying)裝入密封(feng)(feng)袋中(zhong)(zhong)保(bao)(bao)(bao)存(cun)(cun)(cun)。2． 濃(nong)(nong)(nong)洗(xi)(xi)滌液(ye)(ye)(ye)(ye)(ye)(ye)可(ke)(ke)(ke)(ke)能會(hui)有(you)結晶析出(chu)(chu)，稀(xi)(xi)(xi)釋(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)可(ke)(ke)(ke)(ke)在(zai)(zai)(zai)水浴中(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)溫(wen)助溶(rong)，洗(xi)(xi)滌時(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)影響(xiang)結果。3． 各(ge)(ge)步(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)均(jun)應(ying)(ying)使(shi)用(yong)(yong)(yong)(yong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)器，并經常校對(dui)其(qi)準(zhun)(zhun)(zhun)(zhun)(zhun)確性，以(yi)避(bi)免試(shi)(shi)(shi)(shi)(shi)驗(yan)誤差。一(yi)(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)間(jian)＊好控制在(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)鐘(zhong)內(nei)，如標(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)(shu)量(liang)(liang)多(duo)，推薦(jian)使(shi)用(yong)(yong)(yong)(yong)排(pai)槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)。4． 請(qing)(qing)(qing)每次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)的(de)(de)同時(shi)(shi)(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線，＊好做(zuo)復孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如標(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中(zhong)(zhong)待測(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)質(zhi)(zhi)含量(liang)(liang)過高（樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)OD值(zhi)(zhi)(zhi)(zhi)大(da)于(yu)(yu)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)OD值(zhi)(zhi)(zhi)(zhi)），請(qing)(qing)(qing)先用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)釋(shi)(shi)(shi)一(yi)(yi)定(ding)(ding)(ding)倍(bei)(bei)數(shu)(shu)（n倍(bei)(bei)）后(hou)(hou)(hou)再(zai)(zai)(zai)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，計算(suan)(suan)(suan)時(shi)(shi)(shi)(shi)(shi)請(qing)(qing)(qing)＊后(hou)(hou)(hou)乘以(yi)總(zong)稀(xi)(xi)(xi)釋(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)（×n×5）。5． 封(feng)(feng)板(ban)膜(mo)只限一(yi)(yi)次(ci)(ci)性使(shi)用(yong)(yong)(yong)(yong)，以(yi)避(bi)免交(jiao)叉污染。6． 底物(wu)(wu)(wu)請(qing)(qing)(qing)避(bi)光保(bao)(bao)(bao)存(cun)(cun)(cun)。7． 嚴格按(an)(an)(an)照說(shuo)明(ming)書(shu)的(de)(de)操作進(jin)(jin)行，試(shi)(shi)(shi)(shi)(shi)驗(yan)結果判(pan)定(ding)(ding)(ding)必須(xu)以(yi)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)儀讀數(shu)(shu)為(wei)(wei)準(zhun)(zhun)(zhun)(zhun)(zhun).8． 所(suo)有(you)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)，洗(xi)(xi)滌液(ye)(ye)(ye)(ye)(ye)(ye)和(he)(he)各(ge)(ge)種廢棄物(wu)(wu)(wu)都(dou)應(ying)(ying)按(an)(an)(an)傳染物(wu)(wu)(wu)處理。9． 本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)不(bu)(bu)(bu)(bu)同批(pi)(pi)號(hao)組(zu)(zu)分(fen)(fen)(fen)(fen)(fen)不(bu)(bu)(bu)(bu)得(de)混(hun)用(yong)(yong)(yong)(yong)。10. 如與英文說(shuo)明(ming)書(shu)有(you)異，以(yi)英文說(shuo)明(ming)書(shu)為(wei)(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)。【計算(suan)(suan)(suan)】 以(yi)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)為(wei)(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)繪(hui)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線，根據樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)OD 值(zhi)(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線查(cha)(cha)出(chu)(chu)相應(ying)(ying)的(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)；再(zai)(zai)(zai)乘以(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi) 倍(bei)(bei)數(shu)(shu)；或用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)與OD值(zhi)(zhi)(zhi)(zhi)計算(suan)(suan)(suan)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)(zhun)曲線的(de)(de)直線回(hui)歸方(fang)程式(shi)，將樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)OD值(zhi)(zhi)(zhi)(zhi) 代入方(fang)程式(shi)，計算(suan)(suan)(suan)出(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)，再(zai)(zai)(zai)乘以(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi) 倍(bei)(bei)數(shu)(shu)，即(ji)為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)實際濃(nong)(nong)(nong)度(du)(du)(du)。 【試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)組(zu)(zu)成(cheng)】試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)組(zu)(zu)成(cheng)48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)保(bao)(bao)(bao)存(cun)(cun)(cun)說(shuo)明(ming)書(shu)1份(fen)1份(fen)密封(feng)(feng)袋1個1個封(feng)(feng)板(ban)膜(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)2片(pian)（96）2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)板(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)1.5ml×1瓶(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)顯色(se)(se)劑(ji)(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)顯色(se)(se)劑(ji)(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)終止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)3ml×1瓶(ping)6ml×1瓶(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)注(zhu)意(yi)：使(shi)用(yong)(yong)(yong)(yong)前請(qing)(qing)(qing)檢(jian)(jian)查(cha)(cha)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)簽(qian)和(he)(he)數(shu)(shu)量(liang)(liang)與表格是(shi)否一(yi)(yi)致。【精(jing)密度(du)(du)(du)】精(jing)密度(du)(du)(du)用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)的(de)(de)變(bian)異系數(shu)(shu)CV 表示。CV（%） = SD/mean×100批(pi)(pi)內(nei)差：取(qu)(qu)同批(pi)(pi)次(ci)(ci)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)對(dui)低、中(zhong)(zhong)、高值(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)進(jin)(jin)行定(ding)(ding)(ding)量(liang)(liang)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，每份(fen)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)連續(xu)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)20 次(ci)(ci)，分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)計算(suan)(suan)(suan)不(bu)(bu)(bu)(bu)同濃(nong)(nong)(nong)度(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)平均(jun)值(zhi)(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)(zhi)。批(pi)(pi)內(nei)差： CV<9%批(pi)(pi)間(jian)差：選取(qu)(qu)3 個不(bu)(bu)(bu)(bu)同批(pi)(pi)次(ci)(ci)的(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)對(dui)低、中(zhong)(zhong)、高值(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)進(jin)(jin)行定(ding)(ding)(ding)量(liang)(liang)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，每個樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)使(shi)用(yong)(yong)(yong)(yong)同一(yi)(yi)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)重(zhong)(zhong)復測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)10 次(ci)(ci)，分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)計算(suan)(suan)(suan)不(bu)(bu)(bu)(bu)同濃(nong)(nong)(nong)度(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)平均(jun)值(zhi)(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)(zhi)。批(pi)(pi)間(jian)差： CV<11% 【特(te)異性】本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)識別(bie)(bie)(bie)(bie)(bie)(bie)(bie)天然和(he)(he)重(zhong)(zhong)組(zu)(zu)微(wei)生物(wu)(wu)(wu)硝酸(suan)鹽還原(yuan)酶(mei)(mei)(mei)（Nar），經檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)與其(qi)它(ta)相似(si)物(wu)(wu)(wu)質(zhi)(zhi)無明(ming)顯交(jiao)叉反(fan)應(ying)(ying)。由于(yu)(yu)受到(dao)技(ji)術及(ji)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)來(lai)源(yuan)的(de)(de)限制，不(bu)(bu)(bu)(bu)可(ke)(ke)(ke)(ke)能完成(cheng)所(suo)有(you)相關或相似(si)物(wu)(wu)(wu)質(zhi)(zhi)交(jiao)叉反(fan)應(ying)(ying)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，因(yin)此(ci)本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)有(you)可(ke)(ke)(ke)(ke)能與未經檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)其(qi)它(ta)物(wu)(wu)(wu)質(zhi)(zhi)有(you)交(jiao)叉反(fan)應(ying)(ying)。 【穩定(ding)(ding)(ding)性】試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)在(zai)(zai)(zai)有(you)效(xiao)期內(nei)務必按(an)(an)(an)推薦(jian)溫(wen)度(du)(du)(du)保(bao)(bao)(bao)存(cun)(cun)(cun)，活性降低率小(xiao)于(yu)(yu)5%。為(wei)(wei)減小(xiao)外部因(yin)素對(dui)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)破壞前后(hou)(hou)(hou)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)值(zhi)(zhi)(zhi)(zhi)的(de)(de)影響(xiang)，實驗(yan)室(shi)的(de)(de)環(huan)境條(tiao)件需盡(jin)量(liang)(liang)保(bao)(bao)(bao)持一(yi)(yi)致，尤其(qi)是(shi)實驗(yan)室(shi)內(nei)溫(wen)度(du)(du)(du)、濕度(du)(du)(du)及(ji)溫(wen)育條(tiao)件。其(qi)次(ci)(ci)由同一(yi)(yi)實驗(yan)員來(lai)進(jin)(jin)行操作可(ke)(ke)(ke)(ke)減少人為(wei)(wei)誤差。
牛胃(wei)泌素(su)抑(yi)制(zhi)(zhi)肽(GIP)ELISA 試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)文名稱(cheng)牛胃(wei)泌素(su)抑(yi)制(zhi)(zhi)肽(GIP)ELISA 試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)規格96T/48T樣(yang)(yang)(yang)(yang)本(ben)類(lei)型血(xue)(xue)清、血(xue)(xue)漿、細胞(bao)上清液(ye)(ye)(ye)(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)(ye)(ye)(ye)、體液(ye)(ye)(ye)(ye)(ye)(ye)(ye)、灌(guan)洗(xi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)等使(shi)用范(fan)圍(wei)科研(yan)實驗，不(bu)(bu)得用于(yu)臨床診斷反應(ying)(ying)(ying)(ying)時(shi)(shi)間(jian)1-5h試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)保存(cun)(cun)2-8℃有效(xiao)期(qi)6個(ge)月貨期(qi)3-5天(tian)【操(cao)(cao)作(zuo)步(bu)驟(zou)】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)與(yu)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)：在(zai)(zai)(zai)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)(ban)(ban)上設標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)一、第(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)100μl，然(ran)后(hou)(hou)(hou)(hou)(hou)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)一、第(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)(yun)；然(ran)后(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)一孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)(qu)100μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)(yun)；然(ran)后(hou)(hou)(hou)(hou)(hou)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)先(xian)各(ge)(ge)取(qu)(qu)50μl棄掉(diao)，再(zai)各(ge)(ge)取(qu)(qu)50μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)50ul，混(hun)(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)(yun)；混(hun)(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)(yun)后(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)(qu)50μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)(yun)后(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)取(qu)(qu)50μl加(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)九(jiu)、第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)(yun)后(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)(qu)50μl棄掉(diao)。（稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)量(liang)(liang)都為(wei)(wei)(wei)50μl，濃(nong)(nong)度(du)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)為(wei)(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)設空(kong)(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)(kong)白(bai)對照(zhao)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)及(ji)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)劑(ji)(ji)(ji)，其(qi)余(yu)各(ge)(ge)步(bu)操(cao)(cao)作(zuo)相同(tong)(tong)(tong)）、待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)(zai)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)(ban)(ban)上待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)先(xian)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)40μl，然(ran)后(hou)(hou)(hou)(hou)(hou)再(zai)加(jia)(jia)(jia)(jia)待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)＊終稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)度(du)為(wei)(wei)(wei)5倍(bei)(bei)(bei)(bei)(bei)）。加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)加(jia)(jia)(jia)(jia)于(yu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部，盡量(liang)(liang)不(bu)(bu)觸及(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁(bi)，輕輕晃動混(hun)(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)(yun)。3. 溫育：用封(feng)(feng)(feng)(feng)(feng)板(ban)(ban)(ban)膜(mo)封(feng)(feng)(feng)(feng)(feng)板(ban)(ban)(ban)后(hou)(hou)(hou)(hou)(hou)置(zhi)37℃溫育30分(fen)(fen)(fen)鐘(zhong)。4. 配(pei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)：將(jiang)30（48T的(de)(de)(de)(de)(de)(de)20倍(bei)(bei)(bei)(bei)(bei)）倍(bei)(bei)(bei)(bei)(bei)濃(nong)(nong)縮洗(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)用蒸餾(liu)水(shui)30（48T的(de)(de)(de)(de)(de)(de)20倍(bei)(bei)(bei)(bei)(bei)）倍(bei)(bei)(bei)(bei)(bei)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)備用。5. 洗(xi)滌(di)：小(xiao)心揭掉(diao)封(feng)(feng)(feng)(feng)(feng)板(ban)(ban)(ban)膜(mo)，棄去(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體，甩干，每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿洗(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)，靜置(zhi)30秒(miao)后(hou)(hou)(hou)(hou)(hou)棄去(qu)，如(ru)(ru)此(ci)(ci)重復(fu)5次(ci)(ci)，拍(pai)干。6. 加(jia)(jia)(jia)(jia)酶(mei)(mei)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)劑(ji)(ji)(ji)50μl，空(kong)(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)除(chu)外。7. 溫育：操(cao)(cao)作(zuo)同(tong)(tong)(tong)3。8. 洗(xi)滌(di)：操(cao)(cao)作(zuo)同(tong)(tong)(tong)5。9. 顯色(se)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)(jia)(jia)入顯色(se)劑(ji)(ji)(ji)A50μl，再(zai)加(jia)(jia)(jia)(jia)入顯色(se)劑(ji)(ji)(ji)B50μl，輕輕震蕩(dang)混(hun)(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)(yun)，37℃避(bi)(bi)光顯色(se)15分(fen)(fen)(fen)鐘(zhong). 10. 終止(zhi)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)終止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終止(zhi)反應(ying)(ying)(ying)(ying)（此(ci)(ci)時(shi)(shi)藍(lan)色(se)立轉黃色(se)）。11. 測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)：以(yi)(yi)(yi)空(kong)(kong)白(bai)空(kong)(kong)調零(ling)，450nm波長依序測(ce)(ce)(ce)量(liang)(liang)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)吸光度(du)（OD值(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)應(ying)(ying)(ying)(ying)在(zai)(zai)(zai)加(jia)(jia)(jia)(jia)終止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)鐘(zhong)以(yi)(yi)(yi)內(nei)(nei)進(jin)(jin)(jin)行(xing)(xing)。牛胃(wei)泌素(su)抑(yi)制(zhi)(zhi)肽(GIP)ELISA 試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)【注意事項(xiang)】1． 試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)從(cong)冷藏環境中(zhong)(zhong)(zhong)(zhong)(zhong)取(qu)(qu)出應(ying)(ying)(ying)(ying)在(zai)(zai)(zai)室(shi)(shi)溫平(ping)(ping)(ping)衡15-30分(fen)(fen)(fen)鐘(zhong)后(hou)(hou)(hou)(hou)(hou)方可使(shi)用，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)(ban)(ban)開封(feng)(feng)(feng)(feng)(feng)后(hou)(hou)(hou)(hou)(hou)如(ru)(ru)未(wei)用完，板(ban)(ban)(ban)條(tiao)應(ying)(ying)(ying)(ying)裝入密封(feng)(feng)(feng)(feng)(feng)袋中(zhong)(zhong)(zhong)(zhong)(zhong)保存(cun)(cun)。2． 濃(nong)(nong)洗(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)可能會有結(jie)晶析出，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)時(shi)(shi)可在(zai)(zai)(zai)水(shui)浴中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)溫助溶，洗(xi)滌(di)時(shi)(shi)不(bu)(bu)影(ying)響結(jie)果(guo)。3． 各(ge)(ge)步(bu)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)均應(ying)(ying)(ying)(ying)使(shi)用加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)器，并(bing)經(jing)常(chang)校對其(qi)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)，以(yi)(yi)(yi)避(bi)(bi)免試(shi)(shi)驗誤差(cha)。一次(ci)(ci)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)時(shi)(shi)間(jian)＊好控制(zhi)(zhi)在(zai)(zai)(zai)5分(fen)(fen)(fen)鐘(zhong)內(nei)(nei)，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)數(shu)(shu)量(liang)(liang)多，推(tui)薦(jian)使(shi)用排槍加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)。4． 請(qing)(qing)每(mei)(mei)次(ci)(ci)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)同(tong)(tong)(tong)時(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線，＊好做復(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)待測(ce)(ce)(ce)物(wu)(wu)質(zhi)含量(liang)(liang)過(guo)高（樣(yang)(yang)(yang)(yang)本(ben)OD值(zhi)(zhi)(zhi)大(da)于(yu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)一孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)），請(qing)(qing)先(xian)用樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)一定(ding)(ding)(ding)(ding)倍(bei)(bei)(bei)(bei)(bei)數(shu)(shu)（n倍(bei)(bei)(bei)(bei)(bei)）后(hou)(hou)(hou)(hou)(hou)再(zai)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)，計(ji)(ji)算時(shi)(shi)請(qing)(qing)＊后(hou)(hou)(hou)(hou)(hou)乘(cheng)以(yi)(yi)(yi)總稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)(bei)(bei)數(shu)(shu)（×n×5）。5． 封(feng)(feng)(feng)(feng)(feng)板(ban)(ban)(ban)膜(mo)只(zhi)限一次(ci)(ci)性(xing)使(shi)用，以(yi)(yi)(yi)避(bi)(bi)免交叉(cha)污染。6． 底物(wu)(wu)請(qing)(qing)避(bi)(bi)光保存(cun)(cun)。7． 嚴格按(an)(an)照(zhao)說明(ming)(ming)書(shu)的(de)(de)(de)(de)(de)(de)操(cao)(cao)作(zuo)進(jin)(jin)(jin)行(xing)(xing)，試(shi)(shi)驗結(jie)果(guo)判定(ding)(ding)(ding)(ding)必(bi)須以(yi)(yi)(yi)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀數(shu)(shu)為(wei)(wei)(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun).8． 所有樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)，洗(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)和各(ge)(ge)種廢棄物(wu)(wu)都應(ying)(ying)(ying)(ying)按(an)(an)傳染物(wu)(wu)處理。9． 本(ben)試(shi)(shi)劑(ji)(ji)(ji)不(bu)(bu)同(tong)(tong)(tong)批(pi)(pi)號組分(fen)(fen)(fen)不(bu)(bu)得混(hun)(hun)(hun)(hun)用。10. 如(ru)(ru)與(yu)英文說明(ming)(ming)書(shu)有異，以(yi)(yi)(yi)英文說明(ming)(ming)書(shu)為(wei)(wei)(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)。【計(ji)(ji)算】 以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)為(wei)(wei)(wei)橫(heng)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上繪(hui)出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線，根據樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)OD 值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線查出相應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)；再(zai)乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)(bei)(bei)(bei)數(shu)(shu)；或(huo)(huo)用標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)(ji)算出標(biao)(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線的(de)(de)(de)(de)(de)(de)直線回歸(gui)方程式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi) 代入方程式(shi)，計(ji)(ji)算出樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)濃(nong)(nong)度(du)，再(zai)乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)(bei)(bei)(bei)數(shu)(shu)，即(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)實際濃(nong)(nong)度(du)。 【試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)組成(cheng)】試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)組成(cheng)48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)保存(cun)(cun)說明(ming)(ming)書(shu)1份(fen)(fen)1份(fen)(fen)密封(feng)(feng)(feng)(feng)(feng)袋1個(ge)1個(ge)封(feng)(feng)(feng)(feng)(feng)板(ban)(ban)(ban)膜(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)2片（96）2-8℃保存(cun)(cun)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)2-8℃保存(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保存(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)1.5ml×1瓶(ping)2-8℃保存(cun)(cun)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)劑(ji)(ji)(ji)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保存(cun)(cun)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保存(cun)(cun)顯色(se)劑(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保存(cun)(cun)顯色(se)劑(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保存(cun)(cun)終止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)3ml×1瓶(ping)6ml×1瓶(ping)2-8℃保存(cun)(cun)注意：使(shi)用前(qian)請(qing)(qing)檢(jian)(jian)查試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)劑(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和數(shu)(shu)量(liang)(liang)與(yu)表(biao)格是否一致。【精(jing)(jing)密度(du)】精(jing)(jing)密度(du)用樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)變異系數(shu)(shu)CV 表(biao)示。CV（%） = SD/mean×100批(pi)(pi)內(nei)(nei)差(cha)：取(qu)(qu)同(tong)(tong)(tong)批(pi)(pi)次(ci)(ci)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)對低、中(zhong)(zhong)(zhong)(zhong)(zhong)、高值(zhi)(zhi)(zhi)定(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)本(ben)進(jin)(jin)(jin)行(xing)(xing)定(ding)(ding)(ding)(ding)量(liang)(liang)檢(jian)(jian)測(ce)(ce)(ce)，每(mei)(mei)份(fen)(fen)樣(yang)(yang)(yang)(yang)本(ben)連續(xu)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)20 次(ci)(ci)，分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)計(ji)(ji)算不(bu)(bu)同(tong)(tong)(tong)濃(nong)(nong)度(du)樣(yang)(yang)(yang)(yang)本(ben)的(de)(de)(de)(de)(de)(de)平(ping)(ping)(ping)均值(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)。批(pi)(pi)內(nei)(nei)差(cha)： CV<9%批(pi)(pi)間(jian)差(cha)：選取(qu)(qu)3 個(ge)不(bu)(bu)同(tong)(tong)(tong)批(pi)(pi)次(ci)(ci)的(de)(de)(de)(de)(de)(de)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)對低、中(zhong)(zhong)(zhong)(zhong)(zhong)、高值(zhi)(zhi)(zhi)定(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)本(ben)進(jin)(jin)(jin)行(xing)(xing)定(ding)(ding)(ding)(ding)量(liang)(liang)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)，每(mei)(mei)個(ge)樣(yang)(yang)(yang)(yang)本(ben)使(shi)用同(tong)(tong)(tong)一試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)重復(fu)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)10 次(ci)(ci)，分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)計(ji)(ji)算不(bu)(bu)同(tong)(tong)(tong)濃(nong)(nong)度(du)樣(yang)(yang)(yang)(yang)本(ben)的(de)(de)(de)(de)(de)(de)平(ping)(ping)(ping)均值(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)。批(pi)(pi)間(jian)差(cha)： CV<11% 【特異性(xing)】本(ben)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)識別(bie)(bie)(bie)(bie)天(tian)然(ran)和重組微生物(wu)(wu)硝酸(suan)鹽還(huan)原酶(mei)(mei)（Nar），經(jing)檢(jian)(jian)測(ce)(ce)(ce)與(yu)其(qi)它相似(si)物(wu)(wu)質(zhi)無明(ming)(ming)顯交叉(cha)反應(ying)(ying)(ying)(ying)。由于(yu)受(shou)到技術及(ji)樣(yang)(yang)(yang)(yang)本(ben)來(lai)源(yuan)的(de)(de)(de)(de)(de)(de)限制(zhi)(zhi)，不(bu)(bu)可能完成(cheng)所有相關或(huo)(huo)相似(si)物(wu)(wu)質(zhi)交叉(cha)反應(ying)(ying)(ying)(ying)檢(jian)(jian)測(ce)(ce)(ce)，因此(ci)(ci)本(ben)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)有可能與(yu)未(wei)經(jing)檢(jian)(jian)測(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)其(qi)它物(wu)(wu)質(zhi)有交叉(cha)反應(ying)(ying)(ying)(ying)。 【穩定(ding)(ding)(ding)(ding)性(xing)】試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)在(zai)(zai)(zai)有效(xiao)期(qi)內(nei)(nei)務必(bi)按(an)(an)推(tui)薦(jian)溫度(du)保存(cun)(cun)，活性(xing)降低率小(xiao)于(yu)5%。為(wei)(wei)(wei)減(jian)小(xiao)外部因素(su)對試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)破壞前(qian)后(hou)(hou)(hou)(hou)(hou)檢(jian)(jian)測(ce)(ce)(ce)值(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)影(ying)響，實驗室(shi)(shi)的(de)(de)(de)(de)(de)(de)環境條(tiao)件需(xu)盡量(liang)(liang)保持(chi)一致，尤其(qi)是實驗室(shi)(shi)內(nei)(nei)溫度(du)、濕度(du)及(ji)溫育條(tiao)件。其(qi)次(ci)(ci)由同(tong)(tong)(tong)一實驗員來(lai)進(jin)(jin)(jin)行(xing)(xing)操(cao)(cao)作(zuo)可減(jian)少人為(wei)(wei)(wei)誤差(cha)。
牛胃泌(mi)素(su)(GT)ELISA 試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)文(wen)名稱牛胃泌(mi)素(su)(GT)ELISA 試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒規格(ge)96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)類型血清(qing)、血漿、細胞上清(qing)液(ye)(ye)(ye)、尿液(ye)(ye)(ye)、體液(ye)(ye)(ye)、灌洗液(ye)(ye)(ye)等使(shi)(shi)用(yong)(yong)(yong)(yong)范(fan)圍科研(yan)實驗，不(bu)(bu)得用(yong)(yong)(yong)(yong)于(yu)(yu)臨床診斷反(fan)應(ying)(ying)(ying)(ying)時(shi)(shi)(shi)間1-5h試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒保(bao)(bao)存(cun)(cun)(cun)(cun)2-8℃有(you)效期6個(ge)(ge)(ge)月貨期3-5天(tian)【操(cao)作(zuo)(zuo)(zuo)步(bu)驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)稀(xi)釋(shi)(shi)(shi)(shi)與(yu)(yu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)包(bao)(bao)(bao)被板(ban)(ban)(ban)(ban)上設標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)第(di)(di)(di)(di)(di)一(yi)、第(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)100μl，然(ran)后(hou)(hou)(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)(di)一(yi)、第(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)50μl，混勻(yun)(yun)(yun)；然(ran)后(hou)(hou)(hou)(hou)(hou)從第(di)(di)(di)(di)(di)一(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)(ge)(ge)取(qu)100μl分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)第(di)(di)(di)(di)(di)四(si)(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)三、第(di)(di)(di)(di)(di)四(si)(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)50μl，混勻(yun)(yun)(yun)；然(ran)后(hou)(hou)(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)第(di)(di)(di)(di)(di)四(si)(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)先各(ge)(ge)(ge)(ge)取(qu)50μl棄(qi)掉(diao)，再(zai)(zai)各(ge)(ge)(ge)(ge)取(qu)50μl分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)50ul，混勻(yun)(yun)(yun)；混勻(yun)(yun)(yun)后(hou)(hou)(hou)(hou)(hou)從第(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)(ge)(ge)取(qu)50μl分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)50μl，混勻(yun)(yun)(yun)后(hou)(hou)(hou)(hou)(hou)從第(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)取(qu)50μl加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)九、第(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)50μl，混勻(yun)(yun)(yun)后(hou)(hou)(hou)(hou)(hou)從第(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)(ge)(ge)取(qu)50μl棄(qi)掉(diao)。（稀(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)各(ge)(ge)(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)都(dou)為(wei)50μl，濃(nong)度(du)(du)分(fen)(fen)別(bie)(bie)(bie)為(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)別(bie)(bie)(bie)設空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)白(bai)對(dui)照孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)及(ji)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，其(qi)(qi)余各(ge)(ge)(ge)(ge)步(bu)操(cao)作(zuo)(zuo)(zuo)相(xiang)(xiang)同）、待測樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)包(bao)(bao)(bao)被板(ban)(ban)(ban)(ban)上待測樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)先加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)40μl，然(ran)后(hou)(hou)(hou)(hou)(hou)再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)待測樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)＊終(zhong)稀(xi)釋(shi)(shi)(shi)(shi)度(du)(du)為(wei)5倍(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)(yu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部，盡量(liang)不(bu)(bu)觸及(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)輕(qing)晃動混勻(yun)(yun)(yun)。3. 溫育(yu)：用(yong)(yong)(yong)(yong)封(feng)板(ban)(ban)(ban)(ban)膜(mo)封(feng)板(ban)(ban)(ban)(ban)后(hou)(hou)(hou)(hou)(hou)置(zhi)37℃溫育(yu)30分(fen)(fen)鐘。4. 配(pei)液(ye)(ye)(ye)：將(jiang)30（48T的(de)(de)(de)(de)20倍(bei)(bei)）倍(bei)(bei)濃(nong)縮洗滌(di)(di)(di)液(ye)(ye)(ye)用(yong)(yong)(yong)(yong)蒸餾(liu)水(shui)30（48T的(de)(de)(de)(de)20倍(bei)(bei)）倍(bei)(bei)稀(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)備用(yong)(yong)(yong)(yong)。5. 洗滌(di)(di)(di)：小心(xin)揭掉(diao)封(feng)板(ban)(ban)(ban)(ban)膜(mo)，棄(qi)去液(ye)(ye)(ye)體，甩干(gan)，每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)滿(man)洗滌(di)(di)(di)液(ye)(ye)(ye)，靜置(zhi)30秒后(hou)(hou)(hou)(hou)(hou)棄(qi)去，如(ru)(ru)此(ci)重復5次(ci)(ci)(ci)，拍干(gan)。6. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)(mei)：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)50μl，空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外。7. 溫育(yu)：操(cao)作(zuo)(zuo)(zuo)同3。8. 洗滌(di)(di)(di)：操(cao)作(zuo)(zuo)(zuo)同5。9. 顯(xian)(xian)色(se)：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入顯(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)A50μl，再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入顯(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)B50μl，輕(qing)輕(qing)震蕩(dang)混勻(yun)(yun)(yun)，37℃避光顯(xian)(xian)色(se)15分(fen)(fen)鐘. 10. 終(zhong)止(zhi)：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)液(ye)(ye)(ye)50μl，終(zhong)止(zhi)反(fan)應(ying)(ying)(ying)(ying)（此(ci)時(shi)(shi)(shi)藍色(se)立轉黃色(se)）。11. 測定(ding)(ding)(ding)(ding)(ding)：以(yi)(yi)(yi)空(kong)白(bai)空(kong)調(diao)零，450nm波長依序(xu)測量(liang)各(ge)(ge)(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)吸光度(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)(zhi)）。 測定(ding)(ding)(ding)(ding)(ding)應(ying)(ying)(ying)(ying)在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)液(ye)(ye)(ye)后(hou)(hou)(hou)(hou)(hou)15分(fen)(fen)鐘以(yi)(yi)(yi)內(nei)進行。牛胃泌(mi)素(su)(GT)ELISA 試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒【注意事項】1． 試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒從冷藏(zang)環(huan)境中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)取(qu)出應(ying)(ying)(ying)(ying)在(zai)(zai)室(shi)(shi)溫平(ping)衡15-30分(fen)(fen)鐘后(hou)(hou)(hou)(hou)(hou)方(fang)可(ke)使(shi)(shi)用(yong)(yong)(yong)(yong)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)包(bao)(bao)(bao)被板(ban)(ban)(ban)(ban)開(kai)封(feng)后(hou)(hou)(hou)(hou)(hou)如(ru)(ru)未用(yong)(yong)(yong)(yong)完，板(ban)(ban)(ban)(ban)條(tiao)應(ying)(ying)(ying)(ying)裝入密封(feng)袋中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)存(cun)(cun)(cun)(cun)。2． 濃(nong)洗滌(di)(di)(di)液(ye)(ye)(ye)可(ke)能會有(you)結晶析出，稀(xi)釋(shi)(shi)(shi)(shi)時(shi)(shi)(shi)可(ke)在(zai)(zai)水(shui)浴中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)溫助溶，洗滌(di)(di)(di)時(shi)(shi)(shi)不(bu)(bu)影響結果(guo)。3． 各(ge)(ge)(ge)(ge)步(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)均(jun)應(ying)(ying)(ying)(ying)使(shi)(shi)用(yong)(yong)(yong)(yong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)器，并經(jing)常校對(dui)其(qi)(qi)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)，以(yi)(yi)(yi)避免試(shi)(shi)(shi)驗誤差(cha)(cha)(cha)。一(yi)次(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)間＊好(hao)控制在(zai)(zai)5分(fen)(fen)鐘內(nei)，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)本(ben)數(shu)(shu)量(liang)多(duo)，推薦(jian)使(shi)(shi)用(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4． 請每次(ci)(ci)(ci)測定(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)同時(shi)(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)，＊好(hao)做復孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)本(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)待測物質含量(liang)過高(gao)(gao)（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)OD值(zhi)(zhi)(zhi)(zhi)(zhi)大于(yu)(yu)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)一(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)），請先用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)稀(xi)釋(shi)(shi)(shi)(shi)一(yi)定(ding)(ding)(ding)(ding)(ding)倍(bei)(bei)數(shu)(shu)（n倍(bei)(bei)）后(hou)(hou)(hou)(hou)(hou)再(zai)(zai)測定(ding)(ding)(ding)(ding)(ding)，計(ji)算(suan)(suan)時(shi)(shi)(shi)請＊后(hou)(hou)(hou)(hou)(hou)乘以(yi)(yi)(yi)總稀(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)（×n×5）。5． 封(feng)板(ban)(ban)(ban)(ban)膜(mo)只限一(yi)次(ci)(ci)(ci)性(xing)使(shi)(shi)用(yong)(yong)(yong)(yong)，以(yi)(yi)(yi)避免交(jiao)叉污染(ran)。6． 底物請避光保(bao)(bao)存(cun)(cun)(cun)(cun)。7． 嚴格(ge)按(an)照說(shuo)明(ming)(ming)書的(de)(de)(de)(de)操(cao)作(zuo)(zuo)(zuo)進行，試(shi)(shi)(shi)驗結果(guo)判(pan)定(ding)(ding)(ding)(ding)(ding)必(bi)須以(yi)(yi)(yi)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)儀讀數(shu)(shu)為(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun).8． 所有(you)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)，洗滌(di)(di)(di)液(ye)(ye)(ye)和(he)各(ge)(ge)(ge)(ge)種廢棄(qi)物都(dou)應(ying)(ying)(ying)(ying)按(an)傳染(ran)物處理。9． 本(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)不(bu)(bu)同批號組分(fen)(fen)不(bu)(bu)得混用(yong)(yong)(yong)(yong)。10. 如(ru)(ru)與(yu)(yu)英文(wen)說(shuo)明(ming)(ming)書有(you)異，以(yi)(yi)(yi)英文(wen)說(shuo)明(ming)(ming)書為(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)。【計(ji)算(suan)(suan)】 以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物的(de)(de)(de)(de)濃(nong)度(du)(du)為(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)縱(zong)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)紙上繪出標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)，根據樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)OD 值(zhi)(zhi)(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)查出相(xiang)(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)濃(nong)度(du)(du)；再(zai)(zai)乘以(yi)(yi)(yi)稀(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)數(shu)(shu)；或(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物的(de)(de)(de)(de)濃(nong)度(du)(du)與(yu)(yu)OD值(zhi)(zhi)(zhi)(zhi)(zhi)計(ji)算(suan)(suan)出標(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)的(de)(de)(de)(de)直線(xian)(xian)回歸方(fang)程式，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi) 代入方(fang)程式，計(ji)算(suan)(suan)出樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)度(du)(du)，再(zai)(zai)乘以(yi)(yi)(yi)稀(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)數(shu)(shu)，即為(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)實際濃(nong)度(du)(du)。 【試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒組成】試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒組成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)保(bao)(bao)存(cun)(cun)(cun)(cun)說(shuo)明(ming)(ming)書1份1份密封(feng)袋1個(ge)(ge)(ge)1個(ge)(ge)(ge)封(feng)板(ban)(ban)(ban)(ban)膜(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)2片(pian)（96）2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)1.5ml×1瓶(ping)(ping)2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)6 ml×1瓶(ping)(ping)2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)3 ml×1瓶(ping)(ping)6 ml×1瓶(ping)(ping)2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)顯(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)3 ml×1瓶(ping)(ping)6 ml×1瓶(ping)(ping)2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)顯(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)3 ml×1瓶(ping)(ping)6 ml×1瓶(ping)(ping)2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)終(zhong)止(zhi)液(ye)(ye)(ye)3ml×1瓶(ping)(ping)6ml×1瓶(ping)(ping)2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)注意：使(shi)(shi)用(yong)(yong)(yong)(yong)前請檢(jian)(jian)(jian)查試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)簽和(he)數(shu)(shu)量(liang)與(yu)(yu)表格(ge)是否(fou)一(yi)致。【精(jing)密度(du)(du)】精(jing)密度(du)(du)用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)測定(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)變異系數(shu)(shu)CV 表示。CV（%） = SD/mean×100批內(nei)差(cha)(cha)(cha)：取(qu)同批次(ci)(ci)(ci)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒對(dui)低、中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)、高(gao)(gao)值(zhi)(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)進行定(ding)(ding)(ding)(ding)(ding)量(liang)檢(jian)(jian)(jian)測，每份樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)連(lian)續(xu)測定(ding)(ding)(ding)(ding)(ding)20 次(ci)(ci)(ci)，分(fen)(fen)別(bie)(bie)(bie)計(ji)算(suan)(suan)不(bu)(bu)同濃(nong)度(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)的(de)(de)(de)(de)平(ping)均(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)(zhi)(zhi)。批內(nei)差(cha)(cha)(cha)： CV<9%批間差(cha)(cha)(cha)：選取(qu)3 個(ge)(ge)(ge)不(bu)(bu)同批次(ci)(ci)(ci)的(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒分(fen)(fen)別(bie)(bie)(bie)對(dui)低、中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)、高(gao)(gao)值(zhi)(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)進行定(ding)(ding)(ding)(ding)(ding)量(liang)測定(ding)(ding)(ding)(ding)(ding)，每個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)使(shi)(shi)用(yong)(yong)(yong)(yong)同一(yi)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒重復測定(ding)(ding)(ding)(ding)(ding)10 次(ci)(ci)(ci)，分(fen)(fen)別(bie)(bie)(bie)計(ji)算(suan)(suan)不(bu)(bu)同濃(nong)度(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)的(de)(de)(de)(de)平(ping)均(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)(zhi)(zhi)。批間差(cha)(cha)(cha)： CV<11% 【特異性(xing)】本(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒識別(bie)(bie)(bie)天(tian)然(ran)和(he)重組微生物硝(xiao)酸鹽(yan)還(huan)原酶(mei)(mei)(mei)（Nar），經(jing)檢(jian)(jian)(jian)測與(yu)(yu)其(qi)(qi)它相(xiang)(xiang)似物質無明(ming)(ming)顯(xian)(xian)交(jiao)叉反(fan)應(ying)(ying)(ying)(ying)。由于(yu)(yu)受到技(ji)術及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)來源的(de)(de)(de)(de)限制，不(bu)(bu)可(ke)能完成所有(you)相(xiang)(xiang)關或(huo)相(xiang)(xiang)似物質交(jiao)叉反(fan)應(ying)(ying)(ying)(ying)檢(jian)(jian)(jian)測，因此(ci)本(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒有(you)可(ke)能與(yu)(yu)未經(jing)檢(jian)(jian)(jian)測的(de)(de)(de)(de)其(qi)(qi)它物質有(you)交(jiao)叉反(fan)應(ying)(ying)(ying)(ying)。 【穩定(ding)(ding)(ding)(ding)(ding)性(xing)】試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒在(zai)(zai)有(you)效期內(nei)務必(bi)按(an)推薦(jian)溫度(du)(du)保(bao)(bao)存(cun)(cun)(cun)(cun)，活(huo)性(xing)降低率(lv)小于(yu)(yu)5%。為(wei)減小外部因素(su)對(dui)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒破壞前后(hou)(hou)(hou)(hou)(hou)檢(jian)(jian)(jian)測值(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)影響，實驗室(shi)(shi)的(de)(de)(de)(de)環(huan)境條(tiao)件需盡量(liang)保(bao)(bao)持一(yi)致，尤(you)其(qi)(qi)是實驗室(shi)(shi)內(nei)溫度(du)(du)、濕(shi)度(du)(du)及(ji)溫育(yu)條(tiao)件。其(qi)(qi)次(ci)(ci)(ci)由同一(yi)實驗員來進行操(cao)作(zuo)(zuo)(zuo)可(ke)減少人為(wei)誤差(cha)(cha)(cha)。
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