Overview Cat. No： DM-11A04-G Format： LiquidHost： Homo Class： MonoclonalType： Antibody Specificity： Binding to RSV F protein Concentration： Please check the label on the tube. Purity ≥95% as determined by SDS-PAGE Purification： Protein A Storage buffer： PBS or Tris-Gly Storage conditions： Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Warning ? Not for therapeutic use.
Overview Cat. No:DM-A11A04-M Format Liquid Host Homo Class Monoclonal Type Antibody Specificity Binding to RSV F protein Concentration Please check the label on the tube. Purity ≥95% as determined by SDS-PAGE Purification / Storage buffer PBS or Tris-Gly Storage conditions Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Warning ? Not for therapeutic use.
OverviewCat. No:DM-11A04-G Format: Liquid Host: HomoClass: MonoclonalType: AntibodySpecificity: Binding to RSV F proteinConcentration: Please check the label on the tube. Purity: ≥95% as determined by SDS-PAGE Purification Protein A Storage buffer: PBS or Tris-Gly Storage conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.Warning ? Not for therapeutic use.
快(kuai)(kuai)速(su)(su)高(gao)(gao)(gao)爾(er)基(ji)染(ran)(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(神(shen)(shen)(shen)經(jing)元和(he)(he)(he)(he)(he)膠(jiao)(jiao)質(zhi)(zhi)細(xi)胞(bao))產品(pin)貨號(hao): DM1158 產品(pin)規(gui)格(ge): 6 × 125ml/6 ×250ml產品(pin)簡介:Golgi-Cox浸(jin)(jin)(jin)(jin)染(ran)(ran)(ran)(ran)(ran)(ran)(ran)法(fa)(fa)(fa)是(shi)(shi)研究(jiu)神(shen)(shen)(shen)經(jing)元和(he)(he)(he)(he)(he)膠(jiao)(jiao)質(zhi)(zhi)細(xi)胞(bao)正常和(he)(he)(he)(he)(he)非(fei)(fei)正常形態＊有(you)(you)(you)(you)(you)效(xiao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方法(fa)(fa)(fa)之(zhi)一(yi)(yi)(yi)。 使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)Golgi技術，在(zai)(zai)(zai)(zai)藥物(wu)(wu)(wu)(wu)(wu)處(chu) 理過(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)腦中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)因神(shen)(shen)(shen)經(jing)疾(ji)病死(si)亡的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)病人(ren)腦中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)發(fa)現(xian)(xian)了(le)神(shen)(shen)(shen)經(jing)樹突和(he)(he)(he)(he)(he)樹突微(wei)(wei)小(xiao)(xiao)(xiao)(xiao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)形態改變(bian) 。然(ran)(ran)而Golgi染(ran)(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)法(fa)(fa)(fa)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)不(bu)(bu)可(ke)(ke)(ke)(ke)(ke)(ke)(ke) 靠(kao)(kao)性且(qie)(qie)費時(shi)(shi)(shi)(shi)(shi)(shi)已成(cheng)為(wei)這(zhe)種方法(fa)(fa)(fa)廣(guang)泛(fan)應(ying)(ying)(ying)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)障(zhang)礙。快(kuai)(kuai)速(su)(su)高(gao)(gao)(gao)爾(er)基(ji)染(ran)(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)（神(shen)(shen)(shen)經(jing)元和(he)(he)(he)(he)(he)膠(jiao)(jiao)質(zhi)(zhi)細(xi)胞(bao)） 是(shi)(shi)根據Ramón-Moliner ，Glaser和(he)(he)(he)(he)(he)Van der Loos所(suo)(suo)闡(chan)述的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方法(fa)(fa)(fa)原(yuan) 理設(she)(she)計的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de) 。該(gai)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)不(bu)(bu)僅極大(da)(da)地(di)改進(jin)(jin)(jin)(jin)并(bing)簡化(hua)了(le)Golgi-Cox技術，而且(qie)(qie)被證實(shi)(shi)在(zai)(zai)(zai)(zai)顯(xian)(xian)(xian)示神(shen)(shen)(shen)經(jing)元和(he)(he)(he)(he)(he)膠(jiao)(jiao)質(zhi)(zhi)細(xi)胞(bao)，尤其(qi)(qi)是(shi)(shi)樹 突棘的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)形態細(xi)節極為(wei)靈敏(min)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)靠(kao)(kao)。 生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)生(sheng)(sheng)(sheng)(sheng)(sheng)產的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)快(kuai)(kuai)速(su)(su)高(gao)(gao)(gao)爾(er)基(ji)染(ran)(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)已被廣(guang)泛(fan)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)數(shu)種動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)腦組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)染(ran)(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)。 產品(pin)組(zu)(zu)(zu)(zu)(zu)(zu)成(cheng): 產品(pin)名稱6 × 125ml6×250ml溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)A125ml250ml溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)B125ml250ml溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)C125ml×2250ml×2溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)D125ml250ml溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)E125ml250ml注(zhu)(zhu)：溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)C啟封后(hou)(hou)請(qing)(qing)盡快(kuai)(kuai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完。 需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)但未包括在(zai)(zai)(zai)(zai)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)里(li)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)品(pin)1. 雙(shuang)(shuang)蒸(zheng)(zheng)(zheng)水(shui)(shui)(shui)或(huo)Milli-Q水(shui)(shui)(shui)2. 塑料(liao)(liao)/玻璃(li)管或(huo)瓶(ping) 、琉璃(li)樣(yang)品(pin)回收(shou)器(qi)、天(tian)然(ran)(ran)毛畫筆、滴瓶(ping)、塑料(liao)(liao)鑷子3. 組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)學(xue)(xue)(xue)耗材：明(ming)膠(jiao)(jiao)包被的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)顯(xian)(xian)(xian)微(wei)(wei)鏡(jing)載(zai)玻片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)蓋(gai)(gai)玻片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)、染(ran)(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)罐乙醇(chun)二(er)甲(jia)(jia)苯(ben)(ben)樹膠(jiao)(jiao)封片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)劑(ji)(ji)(ji)光(guang)學(xue)(xue)(xue)顯(xian)(xian)(xian)微(wei)(wei)鏡(jing) 組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)制(zhi)備使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)此試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)前必(bi)(bi)(bi)須(xu)仔細(xi)閱(yue)讀以(yi)(yi)(yi)(yi)(yi)下(xia)(xia)說明(ming)！！l 所(suo)(suo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)容(rong)(rong)器(qi)（＊好(hao)(hao)為(wei)塑料(liao)(liao)材質(zhi)(zhi)） 必(bi)(bi)(bi)須(xu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)蒸(zheng)(zheng)(zheng)餾(liu)水(shui)(shui)(shui)沖(chong)(chong)(chong)洗(xi)干(gan)凈(jing)。l 當溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)A和(he)(he)(he)(he)(he)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)B存(cun)(cun)在(zai)(zai)(zai)(zai)時(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)能(neng)(neng)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)金屬(shu)器(qi)具(ju)。l 保(bao)(bao)(bao)(bao)(bao)(bao)持容(rong)(rong)器(qi)密封。l 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)A和(he)(he)(he)(he)(he)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)B處(chu)理的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)和(he)(he)(he)(he)(he)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)，應(ying)(ying)(ying)該(gai)避光(guang)。l 除(chu)(chu)非(fei)(fei)特別指(zhi)出(chu)， 以(yi)(yi)(yi)(yi)(yi)下(xia)(xia)流程(cheng)需(xu)(xu)(xu)(xu)在(zai)(zai)(zai)(zai)室(shi)(shi)(shi)溫(wen)(wen)下(xia)(xia)完成(cheng)。 快(kuai)(kuai)速(su)(su)高(gao)(gao)(gao)爾(er)基(ji)染(ran)(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(神(shen)(shen)(shen)經(jing)元和(he)(he)(he)(he)(he)膠(jiao)(jiao)質(zhi)(zhi)細(xi)胞(bao)) 1. 殺死(si)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)之(zhi)前對(dui)(dui)實(shi)(shi)驗(yan)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)深(shen)度(du)(du)(du)(du)麻醉。應(ying)(ying)(ying)盡快(kuai)(kuai)地(di)從顱骨中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)取(qu)(qu)出(chu)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)腦（或(huo)死(si)去病人(ren)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)腦），但操作(zuo)時(shi)(shi)(shi)(shi)(shi)(shi)必(bi)(bi)(bi) 須(xu)非(fei)(fei)常小(xiao)(xiao)(xiao)(xiao)心(xin)以(yi)(yi)(yi)(yi)(yi)免損傷或(huo)壓迫組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)。注(zhu)(zhu)意(yi)(yi)(yi)(yi)l 除(chu)(chu)非(fei)(fei)完全必(bi)(bi)(bi)要(yao)(yao)(yao)(yao)(yao)，不(bu)(bu)要(yao)(yao)(yao)(yao)(yao)對(dui)(dui)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)灌注(zhu)(zhu)。如(ru)(ru)果(guo)(guo)確(que)(que)實(shi)(shi)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)對(dui)(dui)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)灌注(zhu)(zhu)（用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)4%的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)多(duo)(duo)聚甲(jia)(jia)醛處(chu)理不(bu)(bu)要(yao)(yao)(yao)(yao)(yao)超過(guo)5分(fen)(fen)(fen)鐘）， 一(yi)(yi)(yi)定不(bu)(bu)要(yao)(yao)(yao)(yao)(yao)對(dui)(dui)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)后(hou)(hou)固定處(chu)理。l 體(ti)(ti)積(ji)較大(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)腦部樣(yang)本(ben)(ben)(ben)包括大(da)(da)鼠(shu)腦部應(ying)(ying)(ying)該(gai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)鋒利(li)(li)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)刀片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)成(cheng)大(da)(da)約10mm厚的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)塊狀。重要(yao)(yao)(yao)(yao)(yao)提(ti)示！2. 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙(shuang)(shuang)蒸(zheng)(zheng)(zheng)水(shui)(shui)(shui)或(huo)Milli-Q水(shui)(shui)(shui)快(kuai)(kuai)速(su)(su)沖(chong)(chong)(chong)掉組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)表面的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血液(ye)(ye)(ye)(ye)(ye)。3. 把(ba)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)浸(jin)(jin)(jin)(jin)泡(pao)在(zai)(zai)(zai)(zai)由溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)A和(he)(he)(he)(he)(he)B等體(ti)(ti)積(ji)混合(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浸(jin)(jin)(jin)(jin)漬液(ye)(ye)(ye)(ye)(ye)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，室(shi)(shi)(shi)溫(wen)(wen)下(xia)(xia)黑(hei)暗(an)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)兩(liang)周(zhou)(zhou)* 。浸(jin)(jin)(jin)(jin)泡(pao)6個(ge)小(xiao)(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)以(yi)(yi)(yi)(yi)(yi)后(hou)(hou)或(huo)次(ci)(ci)(ci)日(ri)更(geng)(geng)(geng)(geng)換新(xin) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浸(jin)(jin)(jin)(jin)漬液(ye)(ye)(ye)(ye)(ye)。*對(dui)(dui)于(yu)(yu)(yu)(yu)大(da)(da)多(duo)(duo)數(shu)情(qing)況浸(jin)(jin)(jin)(jin)泡(pao)2周(zhou)(zhou)是(shi)(shi)足夠的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。然(ran)(ran)而，不(bu)(bu)同(tong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)類型(xing)及組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大(da)(da)小(xiao)(xiao)(xiao)(xiao)不(bu)(bu)同(tong)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)能(neng)(neng)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)延長(chang)或(huo)縮短浸(jin)(jin)(jin)(jin)泡(pao)時(shi)(shi)(shi)(shi)(shi)(shi)間來(lai)達(da) 到(dao)＊佳(jia)效(xiao)果(guo)(guo)。對(dui)(dui)于(yu)(yu)(yu)(yu)每(mei)(mei)(mei)種類型(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浸(jin)(jin)(jin)(jin)泡(pao)時(shi)(shi)(shi)(shi)(shi)(shi)間應(ying)(ying)(ying)該(gai)通過(guo)試(shi)(shi)驗(yan)獲得(de)(de)，但是(shi)(shi)對(dui)(dui)于(yu)(yu)(yu)(yu)大(da)(da)多(duo)(duo)數(shu)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)浸(jin)(jin)(jin)(jin)泡(pao)3周(zhou)(zhou)是(shi)(shi)足夠的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。注(zhu)(zhu)意(yi)(yi)(yi)(yi)， 延長(chang)浸(jin)(jin)(jin)(jin)泡(pao)時(shi)(shi)(shi)(shi)(shi)(shi)間可(ke)(ke)(ke)(ke)(ke)(ke)(ke)能(neng)(neng)增加染(ran)(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)背景。注(zhu)(zhu)意(yi)(yi)(yi)(yi)l 溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)A和(he)(he)(he)(he)(he)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)B的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)液(ye)(ye)(ye)(ye)(ye)至少(shao)(shao)在(zai)(zai)(zai)(zai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)之(zhi)前24小(xiao)(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)配制(zhi)，并(bing)不(bu)(bu)能(neng)(neng)攪(jiao)拌。l 采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)以(yi)(yi)(yi)(yi)(yi)上(shang)方案則不(bu)(bu)會(hui)產生(sheng)(sheng)(sheng)(sheng)(sheng)沉(chen)淀是(shi)(shi)關鍵的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。l 制(zhi)備好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浸(jin)(jin)(jin)(jin)泡(pao)液(ye)(ye)(ye)(ye)(ye)在(zai)(zai)(zai)(zai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)之(zhi)前室(shi)(shi)(shi)溫(wen)(wen)黑(hei)暗(an)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)長(chang)達(da)一(yi)(yi)(yi)個(ge)月。l 每(mei)(mei)(mei)cm3 待研究(jiu)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)至少(shao)(shao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)5ml浸(jin)(jin)(jin)(jin)泡(pao)液(ye)(ye)(ye)(ye)(ye)。注(zhu)(zhu)意(yi)(yi)(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)較少(shao)(shao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浸(jin)(jin)(jin)(jin)泡(pao)液(ye)(ye)(ye)(ye)(ye)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)能(neng)(neng)降低(di)染(ran)(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)靈敏(min)性和(he)(he)(he)(he)(he)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)靠(kao)(kao)性。l 為(wei)取(qu)(qu)得(de)(de)＊佳(jia)結(jie)果(guo)(guo)，在(zai)(zai)(zai)(zai)浸(jin)(jin)(jin)(jin)泡(pao)期(qi)間每(mei)(mei)(mei)周(zhou)(zhou)兩(liang)次(ci)(ci)(ci)對(dui)(dui)裝有(you)(you)(you)(you)(you)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)容(rong)(rong)器(qi)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)輕(qing)輕(qing)地(di)左右(you)旋渦（一(yi)(yi)(yi)定不(bu)(bu)要(yao)(yao)(yao)(yao)(yao)晃動(dong)(dong)！ ）幾秒鐘。Warning溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)A和(he)(he)(he)(he)(he)B（含(han)(han)有(you)(you)(you)(you)(you)升汞，重鉻酸(suan)(suan)鉀和(he)(he)(he)(he)(he)鉻酸(suan)(suan)鉀） 接(jie)觸皮膚是(shi)(shi)有(you)(you)(you)(you)(you)毒(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，如(ru)(ru)果(guo)(guo)吞(tun)咽(yan)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)能(neng)(neng)是(shi)(shi)致命(ming)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。實(shi)(shi)驗(yan)應(ying)(ying)(ying)該(gai)在(zai)(zai)(zai)(zai)化(hua)學(xue)(xue)(xue)通風 櫥中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)完成(cheng)。當操作(zuo)這(zhe)些試(shi)(shi)劑(ji)(ji)(ji)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)穿戴(dai)防護服(fu)，手套和(he)(he)(he)(he)(he)防護面具(ju)/眼鏡(jing)。不(bu)(bu)要(yao)(yao)(yao)(yao)(yao)把(ba)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)A和(he)(he)(he)(he)(he)B的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)廢棄物(wu)(wu)(wu)(wu)(wu)倒進(jin)(jin)(jin)(jin)水(shui)(shui)(shui)槽(cao)(cao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)！把(ba)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)A和(he)(he)(he)(he)(he)B的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)廢棄物(wu)(wu)(wu)(wu)(wu)收(shou)集起來(lai)放到(dao)一(yi)(yi)(yi)個(ge)瓶(ping)子中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，致電(dian)安(an)全辦公室(shi)(shi)(shi)或(huo)有(you)(you)(you)(you)(you)執照的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)專業廢物(wu)(wu)(wu)(wu)(wu)處(chu)理服(fu)務(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)部門處(chu)理些材 料(liao)(liao)。4. 轉(zhuan)移組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)到(dao)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)C中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)室(shi)(shi)(shi)溫(wen)(wen)黑(hei)暗(an)至少(shao)(shao)72小(xiao)(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)（可(ke)(ke)(ke)(ke)(ke)(ke)(ke)長(chang)達(da)1周(zhou)(zhou)） 。24小(xiao)(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)或(huo)次(ci)(ci)(ci)日(ri)至少(shao)(shao)更(geng)(geng)(geng)(geng)換一(yi)(yi)(yi)次(ci)(ci)(ci)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)。5. 在(zai)(zai)(zai)(zai)-20℃到(dao)-22℃的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)條件(jian)下(xia)(xia)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)冷(leng)(leng)凍(dong)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)機將組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)成(cheng)100-200um厚的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)薄片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)，也可(ke)(ke)(ke)(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)其(qi)(qi)它(ta)型(xing)號(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)顯(xian)(xian)(xian)微(wei)(wei)鏡(jing)薄片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian) 機包括滑動(dong)(dong)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)機和(he)(he)(he)(he)(he)振(zhen)動(dong)(dong)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)機 。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)本(ben)(ben)(ben)回收(shou)器(qi)把(ba)樣(yang)本(ben)(ben)(ben)轉(zhuan)移到(dao)含(han)(han)有(you)(you)(you)(you)(you)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)C的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)明(ming)膠(jiao)(jiao)包被的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)顯(xian)(xian)(xian)微(wei)(wei)鏡(jing)載(zai)玻片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)上(shang)。載(zai) 玻片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)上(shang)多(duo)(duo)余的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)吸管吸走并(bing)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)濾(lv)紙吸干(gan)（載(zai)玻片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)上(shang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)必(bi)(bi)(bi)須(xu)盡可(ke)(ke)(ke)(ke)(ke)(ke)(ke)能(neng)(neng)地(di)吸干(gan)否(fou)則切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)將從載(zai)玻片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)上(shang)脫落 下(xia)(xia)來(lai)） 。切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)室(shi)(shi)(shi)溫(wen)(wen)下(xia)(xia)自然(ran)(ran)風干(gan)（不(bu)(bu)能(neng)(neng)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)電(dian)風扇或(huo)電(dian)熱板(ban)使(shi)(shi)其(qi)(qi)干(gan)燥） 。為(wei)取(qu)(qu)得(de)(de)＊佳(jia)結(jie)果(guo)(guo)，應(ying)(ying)(ying)盡快(kuai)(kuai)地(di)完成(cheng)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie) 片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)，制(zhi)備好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)如(ru)(ru)果(guo)(guo)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)于(yu)(yu)(yu)(yu)載(zai)玻片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)盒(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，室(shi)(shi)(shi)溫(wen)(wen)黑(hei)暗(an)條件(jian)下(xia)(xia)＊長(chang)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)3天(tian)。注(zhu)(zhu)意(yi)(yi)(yi)(yi)l 為(wei)避免組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)受冷(leng)(leng)凍(dong)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)機的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)損害(hai)，并(bing)盡可(ke)(ke)(ke)(ke)(ke)(ke)(ke)能(neng)(neng)地(di)保(bao)(bao)(bao)(bao)(bao)(bao)持細(xi)胞(bao)形態學(xue)(xue)(xue)，組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)在(zai)(zai)(zai)(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)冷(leng)(leng)凍(dong)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)之(zhi)前應(ying)(ying)(ying)快(kuai)(kuai)速(su)(su)冰(bing)(bing)(bing)(bing)凍(dong)。 比(bi)如(ru)(ru)， 組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)應(ying)(ying)(ying)按(an)以(yi)(yi)(yi)(yi)(yi)下(xia)(xia)描述盡可(ke)(ke)(ke)(ke)(ke)(ke)(ke)能(neng)(neng)地(di)快(kuai)(kuai)速(su)(su)冰(bing)(bing)(bing)(bing)凍(dong)：把(ba)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)放在(zai)(zai)(zai)(zai)一(yi)(yi)(yi)個(ge)塑料(liao)(liao)匙中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)慢慢地(di)浸(jin)(jin)(jin)(jin)入(ru)含(han)(han)有(you)(you)(you)(you)(you)干(gan)冰(bing)(bing)(bing)(bing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)異(yi)戊(wu)烷中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)預冷(leng)(leng)（為(wei)取(qu)(qu) 得(de)(de)＊佳(jia)結(jie)果(guo)(guo)，溫(wen)(wen)度(du)(du)(du)(du)應(ying)(ying)(ying)保(bao)(bao)(bao)(bao)(bao)(bao)持在(zai)(zai)(zai)(zai)-70℃以(yi)(yi)(yi)(yi)(yi)下(xia)(xia)并(bing)且(qie)(qie)浸(jin)(jin)(jin)(jin)入(ru)過(guo)程(cheng)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)時(shi)(shi)(shi)(shi)(shi)(shi)1分(fen)(fen)(fen)鐘，越慢越好(hao)(hao)） 。組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)完全浸(jin)(jin)(jin)(jin)入(ru)異(yi)戊(wu)烷之(zhi)后(hou)(hou)，使(shi)(shi) 組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)在(zai)(zai)(zai)(zai)異(yi)戊(wu)烷中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)浸(jin)(jin)(jin)(jin)幾秒鐘，然(ran)(ran)后(hou)(hou)再(zai)放置(zhi)(zhi)干(gan)冰(bing)(bing)(bing)(bing)上(shang)1分(fen)(fen)(fen)鐘以(yi)(yi)(yi)(yi)(yi)確(que)(que)保(bao)(bao)(bao)(bao)(bao)(bao)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)能(neng)(neng)很好(hao)(hao)地(di)冰(bing)(bing)(bing)(bing)凍(dong)。進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)之(zhi)前不(bu)(bu)要(yao)(yao)(yao)(yao)(yao)讓組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)融 化(hua)。l 切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)機的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)型(xing)號(hao)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)能(neng)(neng)會(hui)有(you)(you)(you)(you)(you)不(bu)(bu)同(tong)，但所(suo)(suo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)型(xing)號(hao)確(que)(que)保(bao)(bao)(bao)(bao)(bao)(bao)能(neng)(neng)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)厚的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)（比(bi)如(ru)(ru)100um）。l 如(ru)(ru)果(guo)(guo)冰(bing)(bing)(bing)(bing)凍(dong)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)機只有(you)(you)(you)(you)(you)一(yi)(yi)(yi)個(ge)溫(wen)(wen)度(du)(du)(du)(du)設(she)(she)置(zhi)(zhi)，那么在(zai)(zai)(zai)(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)之(zhi)前把(ba)溫(wen)(wen)度(du)(du)(du)(du)設(she)(she)置(zhi)(zhi)在(zai)(zai)(zai)(zai)-22℃至少(shao)(shao)4個(ge)小(xiao)(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)。如(ru)(ru)果(guo)(guo)有(you)(you)(you)(you)(you)兩(liang)個(ge)溫(wen)(wen)度(du)(du)(du)(du) 設(she)(she)置(zhi)(zhi)，那么設(she)(she)置(zhi)(zhi)槽(cao)(cao)內溫(wen)(wen)度(du)(du)(du)(du)比(bi)樣(yang)本(ben)(ben)(ben)溫(wen)(wen)度(du)(du)(du)(du)低(di)1℃ 。請(qing)(qing)注(zhu)(zhu)意(yi)(yi)(yi)(yi)大(da)(da)多(duo)(duo)數(shu)情(qing)況下(xia)(xia)-22℃是(shi)(shi)＊佳(jia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)溫(wen)(wen)度(du)(du)(du)(du)，然(ran)(ran)而，為(wei)了(le)更(geng)(geng)(geng)(geng)順(shun)利(li)(li)地(di) 切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)出(chu)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)并(bing)沒有(you)(you)(you)(you)(you)破(po)碎，不(bu)(bu)同(tong)型(xing)號(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)機和(he)(he)(he)(he)(he)不(bu)(bu)同(tong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)能(neng)(neng)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)更(geng)(geng)(geng)(geng)高(gao)(gao)(gao)或(huo)更(geng)(geng)(geng)(geng)低(di)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)槽(cao)(cao)內溫(wen)(wen)度(du)(du)(du)(du)。l 對(dui)(dui)于(yu)(yu)(yu)(yu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)冰(bing)(bing)(bing)(bing)凍(dong)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)機切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)， 以(yi)(yi)(yi)(yi)(yi)下(xia)(xia)幾點提(ti)示是(shi)(shi)有(you)(you)(you)(you)(you)益(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)： 1 ）用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)蒸(zheng)(zheng)(zheng)餾(liu)水(shui)(shui)(shui)把(ba)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)固定于(yu)(yu)(yu)(yu)樣(yang)本(ben)(ben)(ben)盤(pan)上(shang)，但必(bi)(bi)(bi)須(xu)確(que)(que)保(bao)(bao)(bao)(bao)(bao)(bao)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)沒 有(you)(you)(you)(you)(you)融化(hua)（可(ke)(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)在(zai)(zai)(zai)(zai)干(gan)冰(bing)(bing)(bing)(bing)上(shang)完成(cheng)） 。組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)被固定在(zai)(zai)(zai)(zai)任何(he)(he)類型(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)冰(bing)(bing)(bing)(bing)凍(dong)介質(zhi)(zhi)上(shang)包括OCT ，但要(yao)(yao)(yao)(yao)(yao)避免切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)斷介質(zhi)(zhi)。 不(bu)(bu)要(yao)(yao)(yao)(yao)(yao)在(zai)(zai)(zai)(zai)OCT中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)包埋組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)，如(ru)(ru)果(guo)(guo)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)確(que)(que)實(shi)(shi)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)包埋，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)TFM替代OCT 。2） 組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)固定到(dao)樣(yang)本(ben)(ben)(ben)盤(pan)后(hou)(hou)，放置(zhi)(zhi)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)于(yu)(yu)(yu)(yu) 快(kuai)(kuai)速(su)(su)高(gao)(gao)(gao)爾(er)基(ji)染(ran)(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(神(shen)(shen)(shen)經(jing)元和(he)(he)(he)(he)(he)膠(jiao)(jiao)質(zhi)(zhi)細(xi)胞(bao)) 干(gan)冰(bing)(bing)(bing)(bing)上(shang)10分(fen)(fen)(fen)鐘，然(ran)(ran)后(hou)(hou)立即把(ba)固定有(you)(you)(you)(you)(you)樣(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)本(ben)(ben)(ben)盤(pan)安(an)裝到(dao)冰(bing)(bing)(bing)(bing)凍(dong)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)機上(shang)，等5分(fen)(fen)(fen)鐘之(zhi)后(hou)(hou)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)。3）切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi) 些切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)（不(bu)(bu)要(yao)(yao)(yao)(yao)(yao)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)防卷板(ban)） ，如(ru)(ru)果(guo)(guo)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)溫(wen)(wen)度(du)(du)(du)(du)過(guo)低(di)比(bi)如(ru)(ru)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)顯(xian)(xian)(xian)示出(chu)與刀片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)平(ping)行(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)裂(lie)縫，先等幾分(fen)(fen)(fen)鐘再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)下(xia)(xia)一(yi)(yi)(yi) 個(ge)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)，否(fou)則可(ke)(ke)(ke)(ke)(ke)(ke)(ke)繼續切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)。l 浸(jin)(jin)(jin)(jin)泡(pao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)腦組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)瓊脂(zhi)糖或(huo)明(ming)膠(jiao)(jiao)包埋然(ran)(ran)后(hou)(hou)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)冰(bing)(bing)(bing)(bing)凍(dong)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)機切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)。然(ran)(ran)后(hou)(hou)收(shou)集切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)時(shi)(shi)(shi)(shi)(shi)(shi)（充滿(man)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)槽(cao)(cao)） ，必(bi)(bi)(bi)須(xu)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong) 液(ye)(ye)(ye)(ye)(ye)C 。否(fou)則切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)易干(gan)燥裂(lie)紋(wen)。l 如(ru)(ru)果(guo)(guo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)滑動(dong)(dong)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)機切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)浸(jin)(jin)(jin)(jin)泡(pao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)(zu)(zu)(zu)(zu)織(zhi)(zhi)(zhi)(zhi)(zhi)，樣(yang)本(ben)(ben)(ben)盤(pan)和(he)(he)(he)(he)(he)刀片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)都(dou)需(xu)(xu)(xu)(xu)維持在(zai)(zai)(zai)(zai)較低(di)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)溫(wen)(wen)度(du)(du)(du)(du)（0℃以(yi)(yi)(yi)(yi)(yi)下(xia)(xia)） 按(an)照操作(zuo)手冊的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)描述切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie) 片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)固定在(zai)(zai)(zai)(zai)含(han)(han)有(you)(you)(you)(you)(you)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)C的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)載(zai)玻片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)上(shang)是(shi)(shi)重要(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。VI. 染(ran)(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)步(bu)驟(zou)在(zai)(zai)(zai)(zai)染(ran)(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)過(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)蓋(gai)(gai)蓋(gai)(gai)玻片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)之(zhi)前的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)任何(he)(he)一(yi)(yi)(yi)步(bu)都(dou)不(bu)(bu)要(yao)(yao)(yao)(yao)(yao)讓切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)變(bian)干(gan)1. 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙(shuang)(shuang)蒸(zheng)(zheng)(zheng)水(shui)(shui)(shui)或(huo)Milli-Q水(shui)(shui)(shui)沖(chong)(chong)(chong)洗(xi)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)兩(liang)次(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)4分(fen)(fen)(fen)鐘。2. 把(ba)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)置(zhi)(zhi)于(yu)(yu)(yu)(yu)由1份(fen)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)D ， 1份(fen)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)E和(he)(he)(he)(he)(he)2份(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)雙(shuang)(shuang)蒸(zheng)(zheng)(zheng)水(shui)(shui)(shui)或(huo)Milli-Q水(shui)(shui)(shui)組(zu)(zu)(zu)(zu)(zu)(zu)成(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)液(ye)(ye)(ye)(ye)(ye)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)10分(fen)(fen)(fen)鐘。 比(bi)如(ru)(ru)：溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)D 10ml 溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)E 10ml 雙(shuang)(shuang)蒸(zheng)(zheng)(zheng)水(shui)(shui)(shui) 20ml注(zhu)(zhu)意(yi)(yi)(yi)(yi)l 工作(zuo)液(ye)(ye)(ye)(ye)(ye)＊好(hao)(hao)現(xian)(xian)配現(xian)(xian)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，每(mei)(mei)(mei)100ml工作(zuo)液(ye)(ye)(ye)(ye)(ye)＊多(duo)(duo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)100張(zhang)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)（比(bi)如(ru)(ru)小(xiao)(xiao)(xiao)(xiao)鼠(shu)腦） ， 根據切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大(da)(da)小(xiao)(xiao)(xiao)(xiao)。l 盛有(you)(you)(you)(you)(you)工作(zuo)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)瓶(ping)子或(huo)染(ran)(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)罐必(bi)(bi)(bi)須(xu)蓋(gai)(gai)好(hao)(hao)蓋(gai)(gai)子防止試(shi)(shi)劑(ji)(ji)(ji)蒸(zheng)(zheng)(zheng)發(fa)。l 為(wei)取(qu)(qu)得(de)(de)＊佳(jia)結(jie)果(guo)(guo)，孵育期(qi)間應(ying)(ying)(ying)頻(pin)繁攪(jiao)拌工作(zuo)液(ye)(ye)(ye)(ye)(ye)。3. 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)蒸(zheng)(zheng)(zheng)餾(liu)水(shui)(shui)(shui)或(huo)Milli-Q水(shui)(shui)(shui)沖(chong)(chong)(chong)洗(xi)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)兩(liang)次(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)4分(fen)(fen)(fen)鐘（蒸(zheng)(zheng)(zheng)餾(liu)水(shui)(shui)(shui)應(ying)(ying)(ying)頻(pin)繁更(geng)(geng)(geng)(geng)換新(xin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)）。4. 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)結(jie)晶紫(zi)或(huo)硫(liu)堇對(dui)(dui)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)復染(ran)(ran)(ran)(ran)(ran)(ran)(ran)（可(ke)(ke)(ke)(ke)(ke)(ke)(ke)選(xuan)步(bu)驟(zou)）。注(zhu)(zhu)意(yi)(yi)(yi)(yi)l 對(dui)(dui)于(yu)(yu)(yu)(yu)復染(ran)(ran)(ran)(ran)(ran)(ran)(ran)，延長(chang)步(bu)驟(zou)3的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)時(shi)(shi)(shi)(shi)(shi)(shi)間， 比(bi)如(ru)(ru)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)沖(chong)(chong)(chong)洗(xi)4次(ci)(ci)(ci)每(mei)(mei)(mei)次(ci)(ci)(ci)5分(fen)(fen)(fen)鐘代替原(yuan)來(lai)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)沖(chong)(chong)(chong)洗(xi)2次(ci)(ci)(ci)每(mei)(mei)(mei)次(ci)(ci)(ci)4分(fen)(fen)(fen)鐘，或(huo)者(zhe)更(geng)(geng)(geng)(geng)長(chang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)時(shi)(shi)(shi)(shi)(shi)(shi)間。5. 在(zai)(zai)(zai)(zai)50% ，75%和(he)(he)(he)(he)(he)95%的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)乙醇(chun)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)對(dui)(dui)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)脫水(shui)(shui)(shui)，每(mei)(mei)(mei)個(ge)濃度(du)(du)(du)(du)梯度(du)(du)(du)(du)脫水(shui)(shui)(shui)4分(fen)(fen)(fen)鐘（不(bu)(bu)要(yao)(yao)(yao)(yao)(yao)跳過(guo)任何(he)(he)一(yi)(yi)(yi)個(ge)步(bu)驟(zou)）。6. 然(ran)(ran)后(hou)(hou)在(zai)(zai)(zai)(zai)無水(shui)(shui)(shui)乙醇(chun)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)對(dui)(dui)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)脫水(shui)(shui)(shui)，4次(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)4分(fen)(fen)(fen)鐘（不(bu)(bu)要(yao)(yao)(yao)(yao)(yao)延長(chang)時(shi)(shi)(shi)(shi)(shi)(shi)間）。7. 在(zai)(zai)(zai)(zai)二(er)甲(jia)(jia)苯(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)透明(ming)，3次(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)4分(fen)(fen)(fen)鐘，并(bing)且(qie)(qie)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)樹脂(zhi)封片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)劑(ji)(ji)(ji)對(dui)(dui)蓋(gai)(gai)玻片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)封片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)。注(zhu)(zhu)意(yi)(yi)(yi)(yi)l 蓋(gai)(gai)玻片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)之(zhi)前切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)暫時(shi)(shi)(shi)(shi)(shi)(shi)存(cun)(cun)于(yu)(yu)(yu)(yu)二(er)甲(jia)(jia)苯(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)幾個(ge)小(xiao)(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)。l 為(wei)取(qu)(qu)得(de)(de)＊佳(jia)結(jie)果(guo)(guo)，＊好(hao)(hao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)未稀釋的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)封片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)劑(ji)(ji)(ji)。l 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)Golgi染(ran)(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)切(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)(qie)片(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)(pian)無論何(he)(he)時(shi)(shi)(shi)(shi)(shi)(shi)都(dou)應(ying)(ying)(ying)避光(guang)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)。 注(zhu)(zhu)意(yi)(yi)(yi)(yi)事項(xiang)1. 快(kuai)(kuai)速(su)(su)高(gao)(gao)(gao)爾(er)基(ji)染(ran)(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)（神(shen)(shen)(shen)經(jing)元和(he)(he)(he)(he)(he)膠(jiao)(jiao)質(zhi)(zhi)細(xi)胞(bao)）僅用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)體(ti)(ti)外研究(jiu)，不(bu)(bu)能(neng)(neng)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)診斷或(huo)其(qi)(qi)它(ta)應(ying)(ying)(ying)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。2. 試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)所(suo)(suo)包含(han)(han)有(you)(you)(you)(you)(you)試(shi)(shi)劑(ji)(ji)(ji)是(shi)(shi)有(you)(you)(you)(you)(you)毒(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，吸入(ru)或(huo)接(jie)觸皮膚是(shi)(shi)有(you)(you)(you)(you)(you)害(hai)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，如(ru)(ru)果(guo)(guo)吞(tun)咽(yan)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)能(neng)(neng)是(shi)(shi)致命(ming)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。不(bu)(bu)要(yao)(yao)(yao)(yao)(yao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)嘴吸。避免吸入(ru) 和(he)(he)(he)(he)(he)接(jie)觸皮膚和(he)(he)(he)(he)(he)眼睛。如(ru)(ru)果(guo)(guo)接(jie)觸，立即用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)大(da)(da)量(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)水(shui)(shui)(shui)沖(chong)(chong)(chong)洗(xi)并(bing)求助醫生(sheng)(sheng)(sheng)(sheng)(sheng)。3. 在(zai)(zai)(zai)(zai)化(hua)學(xue)(xue)(xue)通風櫥下(xia)(xia)完成(cheng)實(shi)(shi)驗(yan)。操作(zuo)這(zhe)些試(shi)(shi)劑(ji)(ji)(ji)時(shi)(shi)(shi)(shi)(shi)(shi)須(xu)穿戴(dai)合(he)適的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)防護服(fu)，手套和(he)(he)(he)(he)(he)眼睛和(he)(he)(he)(he)(he)面部防護罩，完成(cheng)實(shi)(shi)驗(yan)之(zhi)后(hou)(hou) 把(ba)手徹底沖(chong)(chong)(chong)洗(xi)干(gan)凈(jing)。 保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)條件(jian): 室(shi)(shi)(shi)溫(wen)(wen)，有(you)(you)(you)(you)(you)效(xiao)期(qi) 12 個(ge)月。 上(shang)海篤瑪(ma)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)科技有(you)(you)(you)(you)(you)限公司
本(ben)(ben)(ben)(ben)(ben)試(shi)劑(ji)(ji)(ji)(ji)盒只(zhi)能用(yong)(yong)于(yu)(yu)(yu)科學研(yan)究，不(bu)得用(yong)(yong)于(yu)(yu)(yu)醫(yi)學診斷人（Human）白(bai)細(xi)胞(bao)介(jie)(jie)(jie)素(su)(su)2（IL-2）ELISA檢(jian)(jian)測(ce)試(shi)劑(ji)(ji)(ji)(ji)盒使(shi)用(yong)(yong)說(shuo)明(ming)(ming)(ming)書檢(jian)(jian)測(ce)原理(li)試(shi)劑(ji)(ji)(ji)(ji)盒采用(yong)(yong)雙(shuang)抗(kang)體一步(bu)夾心(xin)法(fa)(fa)酶聯免(mian)疫吸附試(shi)驗(yan)（ELISA）。往預先包(bao)被白(bai)細(xi)胞(bao)介(jie)(jie)(jie)素(su)(su)2（IL-2）抗(kang)體的(de)(de)(de)(de)包(bao)被微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，依(yi)次(ci)加(jia)(jia)(jia)(jia)(jia)入標(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)、HRP標(biao)(biao)(biao)記(ji)的(de)(de)(de)(de)檢(jian)(jian)測(ce)抗(kang)體，經過(guo)溫育并(bing)徹底洗(xi)(xi)滌(di)(di)(di)。用(yong)(yong)底物TMB顯色，TMB在(zai)過(guo)氧(yang)化(hua)物酶的(de)(de)(de)(de)催化(hua)下(xia)轉(zhuan)(zhuan)化(hua)成藍色，并(bing)在(zai)酸的(de)(de)(de)(de)作用(yong)(yong)下(xia)轉(zhuan)(zhuan)化(hua)成＊終(zhong)的(de)(de)(de)(de)黃色。顏色的(de)(de)(de)(de)深(shen)淺(qian)和(he)(he)樣(yang)(yang)(yang)品(pin)中(zhong)的(de)(de)(de)(de)白(bai)細(xi)胞(bao)介(jie)(jie)(jie)素(su)(su)2（IL-2）呈正(zheng)相(xiang)關。用(yong)(yong)酶標(biao)(biao)(biao)儀在(zai)450nm 波長下(xia)測(ce)定(ding)吸光(guang)度(du)(du)(du)（OD 值），計(ji)算樣(yang)(yang)(yang)品(pin)濃(nong)度(du)(du)(du)。樣(yang)(yang)(yang)品(pin)收(shou)集(ji)、處(chu)理(li)及保(bao)存(cun)方法(fa)(fa)1. 血清：使(shi)用(yong)(yong)不(bu)含(han)熱原和(he)(he)內(nei)毒素(su)(su)的(de)(de)(de)(de)試(shi)管(guan)，操(cao)(cao)(cao)(cao)作過(guo)程(cheng)中(zhong)避(bi)(bi)免(mian)任何細(xi)胞(bao)刺(ci)激，收(shou)集(ji)血液(ye)(ye)(ye)后(hou)(hou)，3000轉(zhuan)(zhuan)離(li)心(xin)10分鐘將(jiang)(jiang)血清和(he)(he)紅(hong)細(xi)胞(bao)迅速小(xiao)(xiao)心(xin)地分離(li)。2. 血漿(jiang)：EDTA、檸檬酸鹽(yan)或(huo)肝(gan)素(su)(su)抗(kang)凝(ning)。3000轉(zhuan)(zhuan)離(li)心(xin)30分鐘取(qu)(qu)上清。3. 細(xi)胞(bao)上清液(ye)(ye)(ye)：3000轉(zhuan)(zhuan)離(li)心(xin)10分鐘去除(chu)顆粒和(he)(he)聚合物。4. 組織勻(yun)漿(jiang)：將(jiang)(jiang)組織加(jia)(jia)(jia)(jia)(jia)入適(shi)量生理(li)鹽(yan)水(shui)(shui)搗碎(sui)。3000轉(zhuan)(zhuan)離(li)心(xin)10分鐘取(qu)(qu)上清。5. 保(bao)存(cun)：如(ru)(ru)果(guo)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)收(shou)集(ji)后(hou)(hou)不(bu)及時檢(jian)(jian)測(ce)，請按(an)(an)一次(ci)用(yong)(yong)量分裝，凍存(cun)于(yu)(yu)(yu)-20℃，避(bi)(bi)免(mian)反復凍融，在(zai)室(shi)溫下(xia)解凍并(bing)確(que)保(bao)樣(yang)(yang)(yang)品(pin)均勻(yun)地充分解凍。自(zi)(zi)備物品(pin)1. 酶標(biao)(biao)(biao)儀（450nm）2. 高(gao)精度(du)(du)(du)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)器及槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒溫箱操(cao)(cao)(cao)(cao)作注(zhu)(zhu)意(yi)事項1. 試(shi)劑(ji)(ji)(ji)(ji)盒保(bao)存(cun)在(zai)2-8℃，使(shi)用(yong)(yong)前室(shi)溫平衡20分鐘。從冰箱取(qu)(qu)出(chu)的(de)(de)(de)(de)濃(nong)縮洗(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)會有(you)結(jie)晶，這屬于(yu)(yu)(yu)正(zheng)常現象，水(shui)(shui)浴加(jia)(jia)(jia)(jia)(jia)熱使(shi)結(jie)晶完(wan)全溶(rong)解后(hou)(hou)再(zai)使(shi)用(yong)(yong)。2. 實驗(yan)中(zhong)不(bu)用(yong)(yong)的(de)(de)(de)(de)板(ban)(ban)(ban)(ban)(ban)(ban)(ban)條應立即(ji)放回(hui)(hui)(hui)自(zi)(zi)封(feng)袋(dai)中(zhong)，密(mi)封(feng)（低(di)溫干(gan)燥）保(bao)存(cun)。3. 濃(nong)度(du)(du)(du)為0的(de)(de)(de)(de)S0號(hao)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)即(ji)可視為陰性(xing)(xing)對照(zhao)(zhao)或(huo)者(zhe)空(kong)白(bai)；按(an)(an)照(zhao)(zhao)說(shuo)明(ming)(ming)(ming)書操(cao)(cao)(cao)(cao)作時樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)已經稀釋(shi)(shi)(shi)5倍，＊終(zhong)結(jie)果(guo)乘以5才是樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)實際濃(nong)度(du)(du)(du)。4. 嚴格(ge)按(an)(an)照(zhao)(zhao)說(shuo)明(ming)(ming)(ming)書中(zhong)標(biao)(biao)(biao)明(ming)(ming)(ming)的(de)(de)(de)(de)時間(jian)、加(jia)(jia)(jia)(jia)(jia)液(ye)(ye)(ye)量及順序進(jin)行(xing)溫育操(cao)(cao)(cao)(cao)作。5. 所(suo)有(you)液(ye)(ye)(ye)體組分使(shi)用(yong)(yong)前充分搖勻(yun)。試(shi)劑(ji)(ji)(ji)(ji)盒組成名稱96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置備注(zhu)(zhu)微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶標(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)(ban)(ban)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條無(wu)(wu)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)0.3mL*6管(guan)0.3mL*6管(guan)無(wu)(wu)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)稀釋(shi)(shi)(shi)液(ye)(ye)(ye)6mL3mL無(wu)(wu)檢(jian)(jian)測(ce)抗(kang)體-HRP10mL5mL無(wu)(wu)20×洗(xi)(xi)滌(di)(di)(di)緩沖液(ye)(ye)(ye)25mL15mL按(an)(an)說(shuo)明(ming)(ming)(ming)書進(jin)行(xing)稀釋(shi)(shi)(shi)底物A6mL3mL無(wu)(wu)底物B6mL3mL無(wu)(wu)終(zhong)止(zhi)液(ye)(ye)(ye)6mL3mL無(wu)(wu)封(feng)板(ban)(ban)(ban)(ban)(ban)(ban)(ban)膜2張(zhang)(zhang)2張(zhang)(zhang)無(wu)(wu)說(shuo)明(ming)(ming)(ming)書1份(fen)1份(fen)無(wu)(wu)自(zi)(zi)封(feng)袋(dai)1個1個無(wu)(wu)注(zhu)(zhu)：標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)（S0-S5）濃(nong)度(du)(du)(du)依(yi)次(ci)為：0、7.5、15、30、60、120 pg/mL試(shi)劑(ji)(ji)(ji)(ji)的(de)(de)(de)(de)準(zhun)(zhun)(zhun)備 20×洗(xi)(xi)滌(di)(di)(di)緩沖液(ye)(ye)(ye)的(de)(de)(de)(de)稀釋(shi)(shi)(shi)：蒸餾水(shui)(shui)按(an)(an)1：20稀釋(shi)(shi)(shi)，即(ji)1份(fen)的(de)(de)(de)(de)20×洗(xi)(xi)滌(di)(di)(di)緩沖液(ye)(ye)(ye)加(jia)(jia)(jia)(jia)(jia)19份(fen)的(de)(de)(de)(de)蒸餾水(shui)(shui)。洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)(ban)方法(fa)(fa)1. 手工洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)(ban)：甩(shuai)盡孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)體，每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)滿洗(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)，靜置1min后(hou)(hou)甩(shuai)盡孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)體，在(zai)吸水(shui)(shui)紙(zhi)(zhi)上拍(pai)干(gan)，如(ru)(ru)此洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)(ban)5次(ci)。2. 自(zi)(zi)動洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)(ban)機：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)注(zhu)(zhu)入洗(xi)(xi)液(ye)(ye)(ye)350μL，浸泡1min，洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)(ban)5次(ci)。操(cao)(cao)(cao)(cao)作步(bu)驟1. 從室(shi)溫平衡20min后(hou)(hou)的(de)(de)(de)(de)鋁箔袋(dai)中(zhong)取(qu)(qu)出(chu)所(suo)需板(ban)(ban)(ban)(ban)(ban)(ban)(ban)條，剩余(yu)板(ban)(ban)(ban)(ban)(ban)(ban)(ban)條用(yong)(yong)自(zi)(zi)封(feng)袋(dai)密(mi)封(feng)放回(hui)(hui)(hui)4℃。2. 設置標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)各(ge)加(jia)(jia)(jia)(jia)(jia)不(bu)同濃(nong)度(du)(du)(du)的(de)(de)(de)(de)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)50μL；3. 樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)(jia)待(dai)測(ce)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)10μL，再(zai)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)稀釋(shi)(shi)(shi)液(ye)(ye)(ye)40μL；空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)(jia)(jia)(jia)。4. 除(chu)空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)外，標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)入辣根過(guo)氧(yang)化(hua)物酶（HRP）標(biao)(biao)(biao)記(ji)的(de)(de)(de)(de)檢(jian)(jian)測(ce)抗(kang)體100μL，用(yong)(yong)封(feng)板(ban)(ban)(ban)(ban)(ban)(ban)(ban)膜封(feng)住反應孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，37℃水(shui)(shui)浴鍋或(huo)恒溫箱溫育60min。5. 棄去液(ye)(ye)(ye)體，吸水(shui)(shui)紙(zhi)(zhi)上拍(pai)干(gan)，每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)滿洗(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)，靜置1min，甩(shuai)去洗(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)，吸水(shui)(shui)紙(zhi)(zhi)上拍(pai)干(gan)，如(ru)(ru)此重(zhong)復洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)(ban)5次(ci)（也可用(yong)(yong)洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)(ban)機洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)(ban)）。6. 每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)入底物A、B各(ge)50μL，37℃避(bi)(bi)光(guang)孵(fu)育15min。7. 每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)入終(zhong)止(zhi)液(ye)(ye)(ye)50μL，15min內(nei)，在(zai)450nm波長處(chu)測(ce)定(ding)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)OD值。結(jie)果(guo)判斷 繪(hui)(hui)制標(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)線：在(zai)Excel工作表中(zhong)，以標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)濃(nong)度(du)(du)(du)作橫坐(zuo)標(biao)(biao)(biao)，對應OD值作縱坐(zuo)標(biao)(biao)(biao)，繪(hui)(hui)制出(chu)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)線性(xing)(xing)回(hui)(hui)(hui)歸(gui)(gui)曲(qu)線，按(an)(an)曲(qu)線方程(cheng)計(ji)算各(ge)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)濃(nong)度(du)(du)(du)值。試(shi)劑(ji)(ji)(ji)(ji)盒性(xing)(xing)能1. 準(zhun)(zhun)(zhun)確(que)性(xing)(xing)：標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)線性(xing)(xing)回(hui)(hui)(hui)歸(gui)(gui)與(yu)預期濃(nong)度(du)(du)(du)相(xiang)關系數(shu)(shu)R值，大于(yu)(yu)(yu)等于(yu)(yu)(yu)0.9900。2. 靈敏度(du)(du)(du)：＊低(di)檢(jian)(jian)測(ce)濃(nong)度(du)(du)(du)小(xiao)(xiao)于(yu)(yu)(yu)1.0 pg/mL。3. 特異(yi)性(xing)(xing)：不(bu)與(yu)其它(ta)可溶(rong)性(xing)(xing)結(jie)構類似物交叉反應。4. 重(zhong)復性(xing)(xing)：板(ban)(ban)(ban)(ban)(ban)(ban)(ban)內(nei)、板(ban)(ban)(ban)(ban)(ban)(ban)(ban)間(jian)變異(yi)系數(shu)(shu)均小(xiao)(xiao)于(yu)(yu)(yu)15%。5. 貯藏(zang)：2-8℃，避(bi)(bi)光(guang)防潮保(bao)存(cun)。6. 有(you)效期：6個月免(mian)責聲明(ming)(ming)(ming)1. 試(shi)劑(ji)(ji)(ji)(ji)盒僅供研(yan)究使(shi)用(yong)(yong)，不(bu)得用(yong)(yong)于(yu)(yu)(yu)臨床實驗(yan)或(huo)人體實驗(yan)，否則(ze)所(suo)產生的(de)(de)(de)(de)一切后(hou)(hou)果(guo)，由實驗(yan)者(zhe)承擔(dan)，本(ben)(ben)(ben)(ben)(ben)公司概不(bu)負責。2. 嚴格(ge)按(an)(an)照(zhao)(zhao)說(shuo)明(ming)(ming)(ming)書操(cao)(cao)(cao)(cao)作，實驗(yan)者(zhe)違(wei)反說(shuo)明(ming)(ming)(ming)書操(cao)(cao)(cao)(cao)作，后(hou)(hou)果(guo)由實驗(yan)者(zhe)承擔(dan)。 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Human Interleukin 2 (IL-2) ELISA Kit instruction Intended useThis IL-2 ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of IL-2 in the sample, this IL-2 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IL-2 concentration. The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sample collection and storagesSerum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cyclesPlasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.Materials required but not supplied1. Standard microplate reader(450nm)2. Precision pipettes and Disposable pipette tips.3. 37 ℃ incubatorPrecautions1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.3. Mix all reagents before using.Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)Materials suppliedName96 determinations48 determinationsMicroelisa stripplate12*8strips12*4stripsStandard0.3ml*6tubes0.3ml*6tubesSample Diluent6.0ml3.0mlHRP-Conjugate reagent10.0ml5.0ml20X Wash solution25ml15mlChromogen Solution A6.0ml3.0mlChromogen Solution B6.0ml3.0mlStop Solution6.0ml3.0mlClosure plate membrane22User manual11Sealed bags11Note: Standard (S0 → S5) concentration was followed by:0,7.5,15,30,60,120 pg/mlReagent preparation20×wash solution:Dilute with Distilled or deionized water 1:20.Assay procedure1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.3. Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.Calculation of results1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.5. The sensitivity by this assay is 1.0 pg/ml6. Standard curve Storage： 2-8℃.validity： six months. FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
本(ben)(ben)(ben)試(shi)(shi)劑(ji)盒(he)只能用(yong)(yong)(yong)于(yu)科學研究(jiu)(jiu)，不得用(yong)(yong)(yong)于(yu)醫學診斷人（Human）γ干擾素(su)（IFN-γ）ELISA檢(jian)(jian)測(ce)(ce)試(shi)(shi)劑(ji)盒(he)使(shi)用(yong)(yong)(yong)說明(ming)(ming)書(shu)檢(jian)(jian)測(ce)(ce)原(yuan)理(li)試(shi)(shi)劑(ji)盒(he)采(cai)用(yong)(yong)(yong)雙(shuang)抗(kang)體(ti)一步夾(jia)心(xin)法(fa)酶(mei)(mei)(mei)聯免(mian)疫(yi)吸(xi)附試(shi)(shi)驗(yan)（ELISA）。往預先包被(bei)γ干擾素(su)（IFN-γ）抗(kang)體(ti)的(de)包被(bei)微(wei)孔(kong)(kong)(kong)中(zhong)，依次(ci)加(jia)(jia)入標(biao)本(ben)(ben)(ben)、標(biao)準(zhun)品(pin)(pin)(pin)(pin)、HRP標(biao)記(ji)的(de)檢(jian)(jian)測(ce)(ce)抗(kang)體(ti)，經過(guo)溫(wen)(wen)(wen)(wen)育(yu)并(bing)徹底(di)(di)洗(xi)滌(di)(di)。用(yong)(yong)(yong)底(di)(di)物(wu)TMB顯色(se)，TMB在(zai)(zai)過(guo)氧化物(wu)酶(mei)(mei)(mei)的(de)催化下轉(zhuan)化成(cheng)(cheng)藍色(se)，并(bing)在(zai)(zai)酸的(de)作(zuo)(zuo)用(yong)(yong)(yong)下轉(zhuan)化成(cheng)(cheng)＊終(zhong)的(de)黃(huang)色(se)。顏(yan)色(se)的(de)深淺和樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)中(zhong)的(de)γ干擾素(su)（IFN-γ）呈(cheng)正相關(guan)。用(yong)(yong)(yong)酶(mei)(mei)(mei)標(biao)儀在(zai)(zai)450nm 波長下測(ce)(ce)定吸(xi)光(guang)度(du)(du)(du)（OD 值(zhi)），計算樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)濃度(du)(du)(du)。樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)收集(ji)、處理(li)及保(bao)存方(fang)法(fa)1. 血(xue)(xue)清(qing)(qing)：使(shi)用(yong)(yong)(yong)不含熱(re)原(yuan)和內毒素(su)的(de)試(shi)(shi)管，操(cao)作(zuo)(zuo)過(guo)程(cheng)(cheng)中(zhong)避免(mian)任何細胞刺激(ji)，收集(ji)血(xue)(xue)液(ye)(ye)后，3000轉(zhuan)離(li)心(xin)10分(fen)(fen)(fen)(fen)鐘將血(xue)(xue)清(qing)(qing)和紅細胞迅速小心(xin)地分(fen)(fen)(fen)(fen)離(li)。2. 血(xue)(xue)漿：EDTA、檸檬(meng)酸鹽(yan)或(huo)(huo)肝(gan)素(su)抗(kang)凝(ning)。3000轉(zhuan)離(li)心(xin)30分(fen)(fen)(fen)(fen)鐘取上(shang)(shang)清(qing)(qing)。3. 細胞上(shang)(shang)清(qing)(qing)液(ye)(ye)：3000轉(zhuan)離(li)心(xin)10分(fen)(fen)(fen)(fen)鐘去(qu)(qu)除顆粒和聚合物(wu)。4. 組(zu)(zu)織(zhi)勻漿：將組(zu)(zu)織(zhi)加(jia)(jia)入適量(liang)生理(li)鹽(yan)水(shui)(shui)搗碎。3000轉(zhuan)離(li)心(xin)10分(fen)(fen)(fen)(fen)鐘取上(shang)(shang)清(qing)(qing)。5. 保(bao)存：如(ru)果(guo)樣(yang)(yang)(yang)本(ben)(ben)(ben)收集(ji)后不及時(shi)檢(jian)(jian)測(ce)(ce)，請按(an)(an)一次(ci)用(yong)(yong)(yong)量(liang)分(fen)(fen)(fen)(fen)裝，凍(dong)存于(yu)-20℃，避免(mian)反(fan)(fan)復(fu)(fu)凍(dong)融，在(zai)(zai)室(shi)溫(wen)(wen)(wen)(wen)下解凍(dong)并(bing)確(que)保(bao)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)均勻地充分(fen)(fen)(fen)(fen)解凍(dong)。自(zi)(zi)(zi)備物(wu)品(pin)(pin)(pin)(pin)1. 酶(mei)(mei)(mei)標(biao)儀（450nm）2. 高精度(du)(du)(du)加(jia)(jia)樣(yang)(yang)(yang)器(qi)及槍(qiang)頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒溫(wen)(wen)(wen)(wen)箱(xiang)操(cao)作(zuo)(zuo)注意(yi)事(shi)項1. 試(shi)(shi)劑(ji)盒(he)保(bao)存在(zai)(zai)2-8℃，使(shi)用(yong)(yong)(yong)前室(shi)溫(wen)(wen)(wen)(wen)平衡20分(fen)(fen)(fen)(fen)鐘。從冰箱(xiang)取出的(de)濃縮洗(xi)滌(di)(di)液(ye)(ye)會有結晶，這屬于(yu)正常現象，水(shui)(shui)浴加(jia)(jia)熱(re)使(shi)結晶完全溶解后再使(shi)用(yong)(yong)(yong)。2. 實(shi)驗(yan)中(zhong)不用(yong)(yong)(yong)的(de)板(ban)(ban)(ban)(ban)條(tiao)應立(li)即(ji)放(fang)回(hui)(hui)自(zi)(zi)(zi)封(feng)(feng)(feng)(feng)(feng)袋中(zhong)，密封(feng)(feng)(feng)(feng)(feng)（低溫(wen)(wen)(wen)(wen)干燥）保(bao)存。3. 濃度(du)(du)(du)為(wei)0的(de)S0號標(biao)準(zhun)品(pin)(pin)(pin)(pin)即(ji)可(ke)視為(wei)陰性(xing)對(dui)照或(huo)(huo)者(zhe)(zhe)(zhe)空(kong)(kong)白(bai)(bai)；按(an)(an)照說明(ming)(ming)書(shu)操(cao)作(zuo)(zuo)時(shi)樣(yang)(yang)(yang)本(ben)(ben)(ben)已經稀釋5倍(bei)，＊終(zhong)結果(guo)乘以5才(cai)是樣(yang)(yang)(yang)本(ben)(ben)(ben)實(shi)際濃度(du)(du)(du)。4. 嚴格(ge)按(an)(an)照說明(ming)(ming)書(shu)中(zhong)標(biao)明(ming)(ming)的(de)時(shi)間、加(jia)(jia)液(ye)(ye)量(liang)及順序進(jin)行溫(wen)(wen)(wen)(wen)育(yu)操(cao)作(zuo)(zuo)。5. 所(suo)有液(ye)(ye)體(ti)組(zu)(zu)分(fen)(fen)(fen)(fen)使(shi)用(yong)(yong)(yong)前充分(fen)(fen)(fen)(fen)搖勻。試(shi)(shi)劑(ji)盒(he)組(zu)(zu)成(cheng)(cheng)名稱96孔(kong)(kong)(kong)配(pei)置48孔(kong)(kong)(kong)配(pei)置備注微(wei)孔(kong)(kong)(kong)酶(mei)(mei)(mei)標(biao)板(ban)(ban)(ban)(ban)12孔(kong)(kong)(kong)×8條(tiao)12孔(kong)(kong)(kong)×4條(tiao)無(wu)標(biao)準(zhun)品(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管無(wu)樣(yang)(yang)(yang)本(ben)(ben)(ben)稀釋液(ye)(ye)6mL3mL無(wu)檢(jian)(jian)測(ce)(ce)抗(kang)體(ti)-HRP10mL5mL無(wu)20×洗(xi)滌(di)(di)緩(huan)(huan)沖液(ye)(ye)25mL15mL按(an)(an)說明(ming)(ming)書(shu)進(jin)行稀釋底(di)(di)物(wu)A6mL3mL無(wu)底(di)(di)物(wu)B6mL3mL無(wu)終(zhong)止液(ye)(ye)6mL3mL無(wu)封(feng)(feng)(feng)(feng)(feng)板(ban)(ban)(ban)(ban)膜2張(zhang)2張(zhang)無(wu)說明(ming)(ming)書(shu)1份(fen)(fen)1份(fen)(fen)無(wu)自(zi)(zi)(zi)封(feng)(feng)(feng)(feng)(feng)袋1個(ge)1個(ge)無(wu)注：標(biao)準(zhun)品(pin)(pin)(pin)(pin)（S0-S5）濃度(du)(du)(du)依次(ci)為(wei)：0、50、100、200、400、800 pg/mL試(shi)(shi)劑(ji)的(de)準(zhun)備 20×洗(xi)滌(di)(di)緩(huan)(huan)沖液(ye)(ye)的(de)稀釋：蒸餾(liu)水(shui)(shui)按(an)(an)1：20稀釋，即(ji)1份(fen)(fen)的(de)20×洗(xi)滌(di)(di)緩(huan)(huan)沖液(ye)(ye)加(jia)(jia)19份(fen)(fen)的(de)蒸餾(liu)水(shui)(shui)。洗(xi)板(ban)(ban)(ban)(ban)方(fang)法(fa)1. 手工洗(xi)板(ban)(ban)(ban)(ban)：甩盡(jin)孔(kong)(kong)(kong)內液(ye)(ye)體(ti)，每(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)滿洗(xi)滌(di)(di)液(ye)(ye)，靜(jing)置1min后甩盡(jin)孔(kong)(kong)(kong)內液(ye)(ye)體(ti)，在(zai)(zai)吸(xi)水(shui)(shui)紙上(shang)(shang)拍干，如(ru)此(ci)洗(xi)板(ban)(ban)(ban)(ban)5次(ci)。2. 自(zi)(zi)(zi)動洗(xi)板(ban)(ban)(ban)(ban)機：每(mei)(mei)孔(kong)(kong)(kong)注入洗(xi)液(ye)(ye)350μL，浸泡1min，洗(xi)板(ban)(ban)(ban)(ban)5次(ci)。操(cao)作(zuo)(zuo)步驟1. 從室(shi)溫(wen)(wen)(wen)(wen)平衡20min后的(de)鋁箔袋中(zhong)取出所(suo)需板(ban)(ban)(ban)(ban)條(tiao)，剩余(yu)板(ban)(ban)(ban)(ban)條(tiao)用(yong)(yong)(yong)自(zi)(zi)(zi)封(feng)(feng)(feng)(feng)(feng)袋密封(feng)(feng)(feng)(feng)(feng)放(fang)回(hui)(hui)4℃。2. 設置標(biao)準(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)和樣(yang)(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)，標(biao)準(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)各加(jia)(jia)不同濃度(du)(du)(du)的(de)標(biao)準(zhun)品(pin)(pin)(pin)(pin)50μL；3. 樣(yang)(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)先加(jia)(jia)待(dai)測(ce)(ce)樣(yang)(yang)(yang)本(ben)(ben)(ben)10μL，再加(jia)(jia)樣(yang)(yang)(yang)本(ben)(ben)(ben)稀釋液(ye)(ye)40μL；空(kong)(kong)白(bai)(bai)孔(kong)(kong)(kong)不加(jia)(jia)。4. 除空(kong)(kong)白(bai)(bai)孔(kong)(kong)(kong)外，標(biao)準(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)和樣(yang)(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)中(zhong)每(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)入辣根(gen)過(guo)氧化物(wu)酶(mei)(mei)(mei)（HRP）標(biao)記(ji)的(de)檢(jian)(jian)測(ce)(ce)抗(kang)體(ti)100μL，用(yong)(yong)(yong)封(feng)(feng)(feng)(feng)(feng)板(ban)(ban)(ban)(ban)膜封(feng)(feng)(feng)(feng)(feng)住反(fan)(fan)應孔(kong)(kong)(kong)，37℃水(shui)(shui)浴鍋或(huo)(huo)恒溫(wen)(wen)(wen)(wen)箱(xiang)溫(wen)(wen)(wen)(wen)育(yu)60min。5. 棄去(qu)(qu)液(ye)(ye)體(ti)，吸(xi)水(shui)(shui)紙上(shang)(shang)拍干，每(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)滿洗(xi)滌(di)(di)液(ye)(ye)，靜(jing)置1min，甩去(qu)(qu)洗(xi)滌(di)(di)液(ye)(ye)，吸(xi)水(shui)(shui)紙上(shang)(shang)拍干，如(ru)此(ci)重復(fu)(fu)洗(xi)板(ban)(ban)(ban)(ban)5次(ci)（也可(ke)用(yong)(yong)(yong)洗(xi)板(ban)(ban)(ban)(ban)機洗(xi)板(ban)(ban)(ban)(ban)）。6. 每(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)入底(di)(di)物(wu)A、B各50μL，37℃避光(guang)孵(fu)育(yu)15min。7. 每(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)入終(zhong)止液(ye)(ye)50μL，15min內，在(zai)(zai)450nm波長處測(ce)(ce)定各孔(kong)(kong)(kong)的(de)OD值(zhi)。結果(guo)判(pan)斷 繪制標(biao)準(zhun)曲(qu)(qu)線(xian)(xian)：在(zai)(zai)Excel工作(zuo)(zuo)表中(zhong)，以標(biao)準(zhun)品(pin)(pin)(pin)(pin)濃度(du)(du)(du)作(zuo)(zuo)橫坐標(biao)，對(dui)應OD值(zhi)作(zuo)(zuo)縱坐標(biao)，繪制出標(biao)準(zhun)品(pin)(pin)(pin)(pin)線(xian)(xian)性(xing)回(hui)(hui)歸曲(qu)(qu)線(xian)(xian)，按(an)(an)曲(qu)(qu)線(xian)(xian)方(fang)程(cheng)(cheng)計算各樣(yang)(yang)(yang)本(ben)(ben)(ben)濃度(du)(du)(du)值(zhi)。 試(shi)(shi)劑(ji)盒(he)性(xing)能1. 準(zhun)確(que)性(xing)：標(biao)準(zhun)品(pin)(pin)(pin)(pin)線(xian)(xian)性(xing)回(hui)(hui)歸與預期(qi)濃度(du)(du)(du)相關(guan)系數R值(zhi)，大于(yu)等于(yu)0.9900。2. 靈敏(min)度(du)(du)(du)：＊低檢(jian)(jian)測(ce)(ce)濃度(du)(du)(du)小于(yu)1.0 pg/mL。3. 特異性(xing)：不與其它可(ke)溶性(xing)結構類(lei)似物(wu)交叉反(fan)(fan)應。4. 重復(fu)(fu)性(xing)：板(ban)(ban)(ban)(ban)內、板(ban)(ban)(ban)(ban)間變異系數均小于(yu)15%。5. 貯藏：2-8℃，避光(guang)防潮(chao)保(bao)存。6. 有效期(qi)：6個(ge)月免(mian)責聲明(ming)(ming)1. 試(shi)(shi)劑(ji)盒(he)僅供研究(jiu)(jiu)使(shi)用(yong)(yong)(yong)，不得用(yong)(yong)(yong)于(yu)臨床(chuang)實(shi)驗(yan)或(huo)(huo)人體(ti)實(shi)驗(yan)，否則所(suo)產生的(de)一切后果(guo)，由(you)實(shi)驗(yan)者(zhe)(zhe)(zhe)承擔，本(ben)(ben)(ben)公(gong)司概不負(fu)責。2. 嚴格(ge)按(an)(an)照說明(ming)(ming)書(shu)操(cao)作(zuo)(zuo)，實(shi)驗(yan)者(zhe)(zhe)(zhe)違(wei)反(fan)(fan)說明(ming)(ming)書(shu)操(cao)作(zuo)(zuo)，后果(guo)由(you)實(shi)驗(yan)者(zhe)(zhe)(zhe)承擔。 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Human Interferon γ (IFN-γ) ELISA Kit instruction Intended useThis IFN-γ ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of IFN-γ in the sample, this IFN-γ ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IFN-γ concentration. The concentration of IFN-γ in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sample collection and storagesSerum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cyclesPlasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.Materials required but not supplied1. Standard microplate reader(450nm)2. Precision pipettes and Disposable pipette tips.3. 37 ℃ incubatorPrecautions1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.3. Mix all reagents before using.Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)Materials suppliedName96 determinations48 determinationsMicroelisa stripplate12*8strips12*4stripsStandard0.3ml*6tubes0.3ml*6tubesSample Diluent6.0ml3.0mlHRP-Conjugate reagent10.0ml5.0ml20X Wash solution25ml15mlChromogen Solution A6.0ml3.0mlChromogen Solution B6.0ml3.0mlStop Solution6.0ml3.0mlClosure plate membrane22User manual11Sealed bags11Note: Standard (S0 → S5) concentration was followed by:0,50,100,200,400,800 pg/mlReagent preparation20×wash solution:Dilute with Distilled or deionized water 1:20.Assay procedure1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.3. Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.Calculation of results1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.5. The sensitivity by this assay is 1.0 pg/ml6. Standard curve Storage： 2-8℃.validity： six months. FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Catalog NoIsotypeReactivityApplicationsGene Name Protein NameImmunogen Specificity FormulationSource PurificationDilution Concentration PurityStorage Stability Synonyms Observed Band Cell PathwayTissue SpecificityDM-Ab-13828IgG Human;Mouse;Rat IHC;IFCD68MacrosialinSynthetic Peptide of CD68The antibody detects endogenous CD68 proteins.PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.Monoclonal, MouseThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.WB 500-2000 1:200 IF 1:50-200 1 mg/ml≥90%-20°C/1 yearCD68; Macrosialin; Gp110; CD6837kD[IsoformShort]: Cell membrane; Single-pass type I membrane protein.; [Isoform Long]: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein.Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor celllines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
EID2B antibody Product Information Catalog No. : DM02652 Size: 100μg Form: liquid Purification: Immunogen affinity purified Purity: ≥95% as determined by SDS-PAGEHost: Rabbit Clonality: polyclonal Clone ID: None IsoType: IgGStorage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) Background Acts as a repressor of MYOD-dependent transcription, glucocorticoid receptor-dependent transcription, and muscle differentiation.Immunogen information Immunogen: EP300 interacting inhibitor of differentiation 2B Synonyms: EP300-interacting inhibitor of differentiation 2B (EID-2B)|EID-2-like inhibitor of differentiation 3 (EID-3)|EID2B|EID3 Observed MW: 43 kDa Uniprot ID : Q96D98Application Reactivity: Human Tested Application: ELISA, WB Recommended dilution:WB: 1:500-1:2000
ATF-3 Polyclonal Antibody Catalog No DM-Ab-01554 Isotype IgG Reactivity Human;Mouse;Rat Applications WB;IHC;IF;ELISA Gene Name ATF3Protein Name Cyclic AMP-dependent transcription factor ATF-3 Immunogen The antiserum was produced against synthesized peptide derived from human ATF3. AA range:131-180 Specificity ATF-3 Polyclonal Antibody detects endogenous levels of ATF-3 protein. Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgGPurification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Dilution WB 1:500-2000;IHC-p 1:100-500;IF/ICC 1:100-500;ELISA 1:5000-20000 Concentration 1 mg/ml Purity ≥90% Storage Stability -20°C/1 yearSynonyms ATF3; Cyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3; Activating transcription factor 3 Observed Band 21kD Cell Pathway Nucleus . Tissue Specificity Amygdala,Brain,Cerebellum,Muscle,Function function:This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. ATF subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a homodimer or a heterodimer.,Background activating transcription factor 3(ATF3) Homo sapiens This gene encodes a possible that alternative splicing of this gene may be physiologically important in matters needing attention Avoid repeated freezing and thawing!Usage suggestions This product can be used in immunological reaction related experiments. For Western Blot analysis of 293T-UV cells using ATF-3 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). Western blot analysis of lysates from RAW264.7 cells, using ATF3 Antibody. The lane on the right is blocked with the synthesized peptide. member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is more information, please consult technical personnel. the regulation of target genes. [provided by RefSeq, Apr 2011],Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
322