人(ren)（Human）丙(bing)型肝(gan)(gan)炎(yan)病毒(du)(du)核心(xin)(xin)抗(kang)原(yuan)(yuan)（HCV-cAg）ELISA檢(jian)(jian)(jian)(jian)測(ce)試(shi)(shi)(shi)(shi)劑盒本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑盒只能用(yong)(yong)于(yu)科學研(yan)究，不(bu)得用(yong)(yong)于(yu)醫(yi)學診(zhen)斷產(chan)(chan)品(pin)(pin)(pin)貨號：【DM6990】產(chan)(chan)品(pin)(pin)(pin)規格(ge)：【48T/96T】檢(jian)(jian)(jian)(jian)測(ce)范圍：反應性(xing)：Human檢(jian)(jian)(jian)(jian)測(ce)原(yuan)(yuan)理(li)試(shi)(shi)(shi)(shi)劑盒采(cai)用(yong)(yong)雙抗(kang)原(yuan)(yuan)一(yi)(yi)步夾心(xin)(xin)法酶聯(lian)免疫吸(xi)附試(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)（ELISA）。往預先包(bao)被丙(bing)型肝(gan)(gan)炎(yan)病毒(du)(du)抗(kang)體(ti)(ti)的(de)(de)(de)(de)(de)包(bao)被微孔(kong)(kong)(kong)中(zhong)(zhong)，依次(ci)加(jia)入標(biao)本(ben)(ben)(ben)(ben)、HRP標(biao)記的(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)抗(kang)體(ti)(ti)，經過(guo)溫育并(bing)(bing)徹底(di)洗(xi)(xi)滌(di)。用(yong)(yong)底(di)物(wu)(wu)(wu)TMB顯色(se)，TMB在(zai)過(guo)氧化物(wu)(wu)(wu)酶的(de)(de)(de)(de)(de)催(cui)化下(xia)(xia)轉(zhuan)(zhuan)化成(cheng)藍色(se)，并(bing)(bing)在(zai)酸的(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)用(yong)(yong)下(xia)(xia)轉(zhuan)(zhuan)化成(cheng)＊終(zhong)的(de)(de)(de)(de)(de)黃色(se)。用(yong)(yong)酶標(biao)儀在(zai)450nm波長下(xia)(xia)測(ce)定吸(xi)光(guang)度（OD 值(zhi)(zhi)(zhi)(zhi)），與CUTOFF值(zhi)(zhi)(zhi)(zhi)相比較(jiao)，從(cong)而判(pan)定標(biao)本(ben)(ben)(ben)(ben)中(zhong)(zhong)人(ren)丙(bing)型肝(gan)(gan)炎(yan)病毒(du)(du)核心(xin)(xin)抗(kang)原(yuan)(yuan)（HCV-cAg）的(de)(de)(de)(de)(de)存在(zai)與否。樣(yang)(yang)品(pin)(pin)(pin)收(shou)(shou)集、處理(li)及(ji)保存方法1. 血(xue)清(qing)：使(shi)(shi)(shi)用(yong)(yong)不(bu)含(han)熱(re)原(yuan)(yuan)和內毒(du)(du)素的(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)管，操(cao)(cao)(cao)作(zuo)(zuo)(zuo)過(guo)程中(zhong)(zhong)避(bi)免任何(he)細(xi)胞刺激(ji)，收(shou)(shou)集血(xue)液后，3000轉(zhuan)(zhuan)離(li)心(xin)(xin)10分鐘(zhong)(zhong)將血(xue)清(qing)和紅細(xi)胞迅速小(xiao)心(xin)(xin)地(di)分離(li)。2. 血(xue)漿(jiang)：EDTA、檸檬酸鹽或肝(gan)(gan)素抗(kang)凝。3000轉(zhuan)(zhuan)離(li)心(xin)(xin)30分鐘(zhong)(zhong)取上(shang)(shang)清(qing)。3. 細(xi)胞上(shang)(shang)清(qing)液：3000轉(zhuan)(zhuan)離(li)心(xin)(xin)10分鐘(zhong)(zhong)去除顆粒和聚(ju)合物(wu)(wu)(wu)。4. 組(zu)織(zhi)勻(yun)漿(jiang)：將組(zu)織(zhi)加(jia)入適量生理(li)鹽水搗碎。3000轉(zhuan)(zhuan)離(li)心(xin)(xin)10分鐘(zhong)(zhong)取上(shang)(shang)清(qing)。5. 保存：如果(guo)(guo)樣(yang)(yang)本(ben)(ben)(ben)(ben)收(shou)(shou)集后不(bu)及(ji)時(shi)檢(jian)(jian)(jian)(jian)測(ce)，請按(an)一(yi)(yi)次(ci)用(yong)(yong)量分裝，凍(dong)(dong)存于(yu)-20℃，避(bi)免反復(fu)凍(dong)(dong)融，在(zai)室溫下(xia)(xia)解凍(dong)(dong)并(bing)(bing)確(que)保樣(yang)(yang)品(pin)(pin)(pin)均(jun)(jun)勻(yun)地(di)充分解凍(dong)(dong)。自(zi)備(bei)物(wu)(wu)(wu)品(pin)(pin)(pin)酶標(biao)儀（450nm）高精度加(jia)樣(yang)(yang)器及(ji)槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL37℃恒溫箱(xiang)操(cao)(cao)(cao)作(zuo)(zuo)(zuo)注意事(shi)項試(shi)(shi)(shi)(shi)劑盒保存在(zai)2-8℃，使(shi)(shi)(shi)用(yong)(yong)前(qian)室溫平衡(heng)20分鐘(zhong)(zhong)。從(cong)冰箱(xiang)取出的(de)(de)(de)(de)(de)濃縮洗(xi)(xi)滌(di)液會有結(jie)(jie)(jie)晶，這屬于(yu)正常現象，水浴加(jia)熱(re)使(shi)(shi)(shi)結(jie)(jie)(jie)晶完全溶解后再使(shi)(shi)(shi)用(yong)(yong)。實(shi)(shi)驗(yan)(yan)(yan)中(zhong)(zhong)不(bu)用(yong)(yong)的(de)(de)(de)(de)(de)板(ban)(ban)條應立(li)即放(fang)回(hui)自(zi)封(feng)(feng)袋(dai)中(zhong)(zhong)，密(mi)封(feng)(feng)（低溫干燥）保存。濃度為0的(de)(de)(de)(de)(de)S0號標(biao)準(zhun)品(pin)(pin)(pin)即可視為陰(yin)性(xing)對(dui)(dui)照(zhao)或者(zhe)空白；按(an)照(zhao)說(shuo)明(ming)書(shu)操(cao)(cao)(cao)作(zuo)(zuo)(zuo)時(shi)樣(yang)(yang)本(ben)(ben)(ben)(ben)已經稀(xi)釋5倍(bei)，＊終(zhong)結(jie)(jie)(jie)果(guo)(guo)乘以5才是樣(yang)(yang)本(ben)(ben)(ben)(ben)實(shi)(shi)際(ji)濃度。嚴格(ge)按(an)照(zhao)說(shuo)明(ming)書(shu)中(zhong)(zhong)標(biao)明(ming)的(de)(de)(de)(de)(de)時(shi)間(jian)、加(jia)液量及(ji)順序進行溫育操(cao)(cao)(cao)作(zuo)(zuo)(zuo)。5. 所(suo)有液體(ti)(ti)組(zu)分使(shi)(shi)(shi)用(yong)(yong)前(qian)充分搖勻(yun)。試(shi)(shi)(shi)(shi)劑盒組(zu)成(cheng)名稱96孔(kong)(kong)(kong)配(pei)置48孔(kong)(kong)(kong)配(pei)置備(bei)注微孔(kong)(kong)(kong)酶標(biao)板(ban)(ban)12孔(kong)(kong)(kong)×8條12孔(kong)(kong)(kong)×4條無(wu)(wu)(wu)(wu)標(biao)準(zhun)品(pin)(pin)(pin)0.3mL*6管0.3mL*6管無(wu)(wu)(wu)(wu)樣(yang)(yang)本(ben)(ben)(ben)(ben)稀(xi)釋液6mL3mL無(wu)(wu)(wu)(wu)檢(jian)(jian)(jian)(jian)測(ce)抗(kang)體(ti)(ti)-HRP10mL5mL無(wu)(wu)(wu)(wu)20×洗(xi)(xi)滌(di)緩沖(chong)液25mL15mL按(an)說(shuo)明(ming)書(shu)進行稀(xi)釋底(di)物(wu)(wu)(wu)A6mL3mL無(wu)(wu)(wu)(wu)底(di)物(wu)(wu)(wu)B6mL3mL無(wu)(wu)(wu)(wu)終(zhong)止(zhi)液6mL3mL無(wu)(wu)(wu)(wu)封(feng)(feng)板(ban)(ban)膜(mo)2張(zhang)2張(zhang)無(wu)(wu)(wu)(wu)說(shuo)明(ming)書(shu)1份1份無(wu)(wu)(wu)(wu)自(zi)封(feng)(feng)袋(dai)1個(ge)1個(ge)無(wu)(wu)(wu)(wu)試(shi)(shi)(shi)(shi)劑的(de)(de)(de)(de)(de)準(zhun)備(bei) 20×洗(xi)(xi)滌(di)緩沖(chong)液的(de)(de)(de)(de)(de)稀(xi)釋：蒸餾水按(an)1：20稀(xi)釋，即1份的(de)(de)(de)(de)(de)20×洗(xi)(xi)滌(di)緩沖(chong)液加(jia)19份的(de)(de)(de)(de)(de)蒸餾水。洗(xi)(xi)板(ban)(ban)方法手工洗(xi)(xi)板(ban)(ban)：甩(shuai)盡孔(kong)(kong)(kong)內液體(ti)(ti)，每(mei)(mei)孔(kong)(kong)(kong)加(jia)滿洗(xi)(xi)滌(di)液，靜置1min后甩(shuai)盡孔(kong)(kong)(kong)內液體(ti)(ti)，在(zai)吸(xi)水紙上(shang)(shang)拍干，如此洗(xi)(xi)板(ban)(ban)5次(ci)。自(zi)動洗(xi)(xi)板(ban)(ban)機：每(mei)(mei)孔(kong)(kong)(kong)注入洗(xi)(xi)液350μL，浸(jin)泡1min，洗(xi)(xi)板(ban)(ban)5次(ci)。操(cao)(cao)(cao)作(zuo)(zuo)(zuo)步驟(zou) 結(jie)(jie)(jie)果(guo)(guo)判(pan)斷1. 試(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)有效性(xing)：陽(yang)性(xing)對(dui)(dui)照(zhao)孔(kong)(kong)(kong)OD值(zhi)(zhi)(zhi)(zhi)平均(jun)(jun)值(zhi)(zhi)(zhi)(zhi)≥1.00； 陰(yin)性(xing)對(dui)(dui)照(zhao)孔(kong)(kong)(kong)OD值(zhi)(zhi)(zhi)(zhi)平均(jun)(jun)值(zhi)(zhi)(zhi)(zhi)≤0.15。2. 臨(lin)(lin)界值(zhi)(zhi)(zhi)(zhi)（Cut off）計算：臨(lin)(lin)界值(zhi)(zhi)(zhi)(zhi)=陰(yin)性(xing)對(dui)(dui)照(zhao)孔(kong)(kong)(kong)平均(jun)(jun)值(zhi)(zhi)(zhi)(zhi)+0.153. 陰(yin)性(xing)判(pan)斷：樣(yang)(yang)品(pin)(pin)(pin)OD值(zhi)(zhi)(zhi)(zhi)＜臨(lin)(lin)界值(zhi)(zhi)(zhi)(zhi)（Cut off），樣(yang)(yang)品(pin)(pin)(pin)為陰(yin)性(xing)4. 陽(yang)性(xing)判(pan)斷：樣(yang)(yang)品(pin)(pin)(pin)OD值(zhi)(zhi)(zhi)(zhi)＞臨(lin)(lin)界值(zhi)(zhi)(zhi)(zhi)（Cut off），樣(yang)(yang)品(pin)(pin)(pin)為陽(yang)性(xing)試(shi)(shi)(shi)(shi)劑盒性(xing)能準(zhun)確(que)性(xing)：陽(yang)性(xing)對(dui)(dui)照(zhao)孔(kong)(kong)(kong)OD值(zhi)(zhi)(zhi)(zhi)平均(jun)(jun)值(zhi)(zhi)(zhi)(zhi)≥1.00；陰(yin)性(xing)對(dui)(dui)照(zhao)孔(kong)(kong)(kong)OD值(zhi)(zhi)(zhi)(zhi)平均(jun)(jun)值(zhi)(zhi)(zhi)(zhi)≤0.15，說(shuo)明(ming)試(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)結(jie)(jie)(jie)果(guo)(guo)有效。特異性(xing)：不(bu)與其它可溶性(xing)結(jie)(jie)(jie)構類似物(wu)(wu)(wu)交叉反應。重復(fu)性(xing)：板(ban)(ban)內、板(ban)(ban)間(jian)變(bian)異系(xi)數均(jun)(jun)小(xiao)于(yu)15%。貯藏：2-8℃，避(bi)光(guang)防潮保存。有效期(qi)：6個(ge)月免責聲明(ming)試(shi)(shi)(shi)(shi)劑盒僅供研(yan)究使(shi)(shi)(shi)用(yong)(yong)，不(bu)得用(yong)(yong)于(yu)臨(lin)(lin)床實(shi)(shi)驗(yan)(yan)(yan)或人(ren)體(ti)(ti)實(shi)(shi)驗(yan)(yan)(yan)，否則(ze)所(suo)產(chan)(chan)生的(de)(de)(de)(de)(de)一(yi)(yi)切后果(guo)(guo)，由實(shi)(shi)驗(yan)(yan)(yan)者(zhe)承擔，本(ben)(ben)(ben)(ben)公(gong)司概不(bu)負責。嚴格(ge)按(an)照(zhao)說(shuo)明(ming)書(shu)操(cao)(cao)(cao)作(zuo)(zuo)(zuo)，實(shi)(shi)驗(yan)(yan)(yan)者(zhe)違反說(shuo)明(ming)書(shu)操(cao)(cao)(cao)作(zuo)(zuo)(zuo)，后果(guo)(guo)由實(shi)(shi)驗(yan)(yan)(yan)者(zhe)承擔。技術(shu)支持電話：電子郵件：網址：fllygs.com生產(chan)(chan)廠家：上(shang)(shang)海篤瑪生物(wu)(wu)(wu)科技有限公(gong)司
人(ren)（Human）丙(bing)型肝(gan)(gan)炎(yan)病(bing)毒(du)抗(kang)(kang)體（HCV-Ab）ELISA檢(jian)(jian)(jian)測(ce)試(shi)劑(ji)(ji)(ji)盒(he)本(ben)試(shi)劑(ji)(ji)(ji)盒(he)只能(neng)用(yong)(yong)(yong)于(yu)(yu)(yu)(yu)科學研究(jiu)(jiu)，不得用(yong)(yong)(yong)于(yu)(yu)(yu)(yu)醫學診(zhen)斷產(chan)品(pin)(pin)(pin)貨號：【DM6989】產(chan)品(pin)(pin)(pin)規格(ge)：【48T/96T】檢(jian)(jian)(jian)測(ce)范(fan)圍：反(fan)應(ying)性(xing)(xing)(xing)(xing)(xing)：Human檢(jian)(jian)(jian)測(ce)范(fan)圍：3.65-225 U/L靈敏度(du)： 1.0 U/L檢(jian)(jian)(jian)測(ce)原(yuan)(yuan)理試(shi)劑(ji)(ji)(ji)盒(he)采用(yong)(yong)(yong)間接法酶聯(lian)免疫吸附試(shi)驗(yan)(yan)(yan)(yan)（ELISA）。往預先(xian)包被(bei)人(ren)丙(bing)型肝(gan)(gan)炎(yan)病(bing)毒(du)抗(kang)(kang)原(yuan)(yuan)的(de)(de)(de)(de)(de)包被(bei)微孔(kong)(kong)中，依次(ci)加(jia)(jia)(jia)入標(biao)(biao)(biao)(biao)本(ben)、HRP標(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)抗(kang)(kang)體，經過溫育并徹底(di)洗(xi)(xi)(xi)滌。用(yong)(yong)(yong)底(di)物TMB顯色(se)，TMB在(zai)(zai)過氧化(hua)(hua)物酶的(de)(de)(de)(de)(de)催化(hua)(hua)下轉(zhuan)化(hua)(hua)成(cheng)藍色(se)，并在(zai)(zai)酸的(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)下轉(zhuan)化(hua)(hua)成(cheng)＊終的(de)(de)(de)(de)(de)黃(huang)色(se)。用(yong)(yong)(yong)酶標(biao)(biao)(biao)(biao)儀在(zai)(zai)450nm波長下測(ce)定(ding)吸光度(du)（OD 值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)），與(yu)CUTOFF值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)相(xiang)比較，從(cong)而(er)判定(ding)標(biao)(biao)(biao)(biao)本(ben)中人(ren)丙(bing)型肝(gan)(gan)炎(yan)病(bing)毒(du)抗(kang)(kang)體（HCV-Ab）的(de)(de)(de)(de)(de)存(cun)在(zai)(zai)與(yu)否(fou)。樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)收(shou)集、處理及(ji)保(bao)存(cun)方法1. 血(xue)清(qing)(qing)：使(shi)(shi)用(yong)(yong)(yong)不含(han)熱(re)原(yuan)(yuan)和內(nei)(nei)毒(du)素的(de)(de)(de)(de)(de)試(shi)管，操(cao)作(zuo)(zuo)(zuo)過程中避(bi)免任何(he)細胞(bao)刺(ci)激，收(shou)集血(xue)液(ye)(ye)(ye)后(hou)(hou)(hou)，3000轉(zhuan)離心(xin)10分(fen)鐘(zhong)(zhong)將血(xue)清(qing)(qing)和紅(hong)細胞(bao)迅速小(xiao)心(xin)地分(fen)離。2. 血(xue)漿(jiang)：EDTA、檸檬(meng)酸鹽或肝(gan)(gan)素抗(kang)(kang)凝(ning)。3000轉(zhuan)離心(xin)30分(fen)鐘(zhong)(zhong)取上(shang)(shang)清(qing)(qing)。3. 細胞(bao)上(shang)(shang)清(qing)(qing)液(ye)(ye)(ye)：3000轉(zhuan)離心(xin)10分(fen)鐘(zhong)(zhong)去除顆粒和聚合物。4. 組(zu)織(zhi)勻漿(jiang)：將組(zu)織(zhi)加(jia)(jia)(jia)入適量生理鹽水搗碎(sui)。3000轉(zhuan)離心(xin)10分(fen)鐘(zhong)(zhong)取上(shang)(shang)清(qing)(qing)。5. 保(bao)存(cun)：如果(guo)(guo)(guo)樣(yang)(yang)(yang)(yang)(yang)本(ben)收(shou)集后(hou)(hou)(hou)不及(ji)時(shi)檢(jian)(jian)(jian)測(ce)，請按(an)一次(ci)用(yong)(yong)(yong)量分(fen)裝，凍(dong)存(cun)于(yu)(yu)(yu)(yu)-20℃，避(bi)免反(fan)復凍(dong)融，在(zai)(zai)室溫下解凍(dong)并確(que)保(bao)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)均(jun)勻地充(chong)分(fen)解凍(dong)。自備物品(pin)(pin)(pin)酶標(biao)(biao)(biao)(biao)儀（450nm）高精度(du)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)器及(ji)槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL37℃恒溫箱操(cao)作(zuo)(zuo)(zuo)注意事(shi)項試(shi)劑(ji)(ji)(ji)盒(he)保(bao)存(cun)在(zai)(zai)2-8℃，使(shi)(shi)用(yong)(yong)(yong)前室溫平(ping)衡(heng)20分(fen)鐘(zhong)(zhong)。從(cong)冰(bing)箱取出的(de)(de)(de)(de)(de)濃(nong)(nong)縮(suo)洗(xi)(xi)(xi)滌液(ye)(ye)(ye)會有(you)(you)(you)結晶，這屬于(yu)(yu)(yu)(yu)正常(chang)現(xian)象(xiang)，水浴(yu)加(jia)(jia)(jia)熱(re)使(shi)(shi)結晶完全溶(rong)解后(hou)(hou)(hou)再使(shi)(shi)用(yong)(yong)(yong)。實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)中不用(yong)(yong)(yong)的(de)(de)(de)(de)(de)板(ban)條(tiao)應(ying)立(li)即放回自封袋(dai)中，密封（低溫干燥）保(bao)存(cun)。濃(nong)(nong)度(du)為0的(de)(de)(de)(de)(de)S0號標(biao)(biao)(biao)(biao)準品(pin)(pin)(pin)即可視(shi)為陰(yin)性(xing)(xing)(xing)(xing)(xing)對(dui)(dui)(dui)照(zhao)或者空(kong)白；按(an)照(zhao)說(shuo)明(ming)(ming)書(shu)操(cao)作(zuo)(zuo)(zuo)時(shi)樣(yang)(yang)(yang)(yang)(yang)本(ben)已經稀(xi)釋5倍，＊終結果(guo)(guo)(guo)乘以(yi)5才是(shi)樣(yang)(yang)(yang)(yang)(yang)本(ben)實(shi)(shi)(shi)際濃(nong)(nong)度(du)。嚴(yan)格(ge)按(an)照(zhao)說(shuo)明(ming)(ming)書(shu)中標(biao)(biao)(biao)(biao)明(ming)(ming)的(de)(de)(de)(de)(de)時(shi)間、加(jia)(jia)(jia)液(ye)(ye)(ye)量及(ji)順序進(jin)(jin)行溫育操(cao)作(zuo)(zuo)(zuo)。所(suo)有(you)(you)(you)液(ye)(ye)(ye)體組(zu)分(fen)使(shi)(shi)用(yong)(yong)(yong)前充(chong)分(fen)搖勻。試(shi)劑(ji)(ji)(ji)盒(he)組(zu)成(cheng)名稱(cheng)96孔(kong)(kong)配置48孔(kong)(kong)配置備注微孔(kong)(kong)酶標(biao)(biao)(biao)(biao)板(ban)12孔(kong)(kong)×8條(tiao)12孔(kong)(kong)×4條(tiao)無(wu)(wu)(wu)標(biao)(biao)(biao)(biao)準品(pin)(pin)(pin)0.3mL*6管0.3mL*6管無(wu)(wu)(wu)樣(yang)(yang)(yang)(yang)(yang)本(ben)稀(xi)釋液(ye)(ye)(ye)6mL3mL無(wu)(wu)(wu)檢(jian)(jian)(jian)測(ce)抗(kang)(kang)體-HRP10mL5mL無(wu)(wu)(wu)20×洗(xi)(xi)(xi)滌緩(huan)(huan)沖(chong)液(ye)(ye)(ye)25mL15mL按(an)說(shuo)明(ming)(ming)書(shu)進(jin)(jin)行稀(xi)釋底(di)物A6mL3mL無(wu)(wu)(wu)底(di)物B6mL3mL無(wu)(wu)(wu)終止液(ye)(ye)(ye)6mL3mL無(wu)(wu)(wu)封板(ban)膜2張2張無(wu)(wu)(wu)說(shuo)明(ming)(ming)書(shu)1份1份無(wu)(wu)(wu)自封袋(dai)1個1個無(wu)(wu)(wu)試(shi)劑(ji)(ji)(ji)的(de)(de)(de)(de)(de)準備20×洗(xi)(xi)(xi)滌緩(huan)(huan)沖(chong)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)稀(xi)釋：蒸餾(liu)水按(an)1：20稀(xi)釋，即1份的(de)(de)(de)(de)(de)20×洗(xi)(xi)(xi)滌緩(huan)(huan)沖(chong)液(ye)(ye)(ye)加(jia)(jia)(jia)19份的(de)(de)(de)(de)(de)蒸餾(liu)水。洗(xi)(xi)(xi)板(ban)方法手工洗(xi)(xi)(xi)板(ban)：甩(shuai)盡孔(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)體，每孔(kong)(kong)加(jia)(jia)(jia)滿洗(xi)(xi)(xi)滌液(ye)(ye)(ye)，靜(jing)置1min后(hou)(hou)(hou)甩(shuai)盡孔(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)體，在(zai)(zai)吸水紙(zhi)上(shang)(shang)拍干，如此洗(xi)(xi)(xi)板(ban)5次(ci)。自動洗(xi)(xi)(xi)板(ban)機：每孔(kong)(kong)注入洗(xi)(xi)(xi)液(ye)(ye)(ye)350μL，浸泡1min，洗(xi)(xi)(xi)板(ban)5次(ci)。操(cao)作(zuo)(zuo)(zuo)步驟(zou) 結果(guo)(guo)(guo)判斷1. 試(shi)驗(yan)(yan)(yan)(yan)有(you)(you)(you)效性(xing)(xing)(xing)(xing)(xing)：陽性(xing)(xing)(xing)(xing)(xing)對(dui)(dui)(dui)照(zhao)孔(kong)(kong)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)平(ping)均(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)≥1.00； 陰(yin)性(xing)(xing)(xing)(xing)(xing)對(dui)(dui)(dui)照(zhao)孔(kong)(kong)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)平(ping)均(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)≤0.15。2. 臨(lin)界(jie)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)（Cut off）計(ji)算：臨(lin)界(jie)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)=陰(yin)性(xing)(xing)(xing)(xing)(xing)對(dui)(dui)(dui)照(zhao)孔(kong)(kong)平(ping)均(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)+0.153. 陰(yin)性(xing)(xing)(xing)(xing)(xing)判斷：樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)＜臨(lin)界(jie)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)（Cut off），樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)為陰(yin)性(xing)(xing)(xing)(xing)(xing)4. 陽性(xing)(xing)(xing)(xing)(xing)判斷：樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)＞臨(lin)界(jie)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)（Cut off），樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)為陽性(xing)(xing)(xing)(xing)(xing)試(shi)劑(ji)(ji)(ji)盒(he)性(xing)(xing)(xing)(xing)(xing)能(neng)準確(que)性(xing)(xing)(xing)(xing)(xing)：陽性(xing)(xing)(xing)(xing)(xing)對(dui)(dui)(dui)照(zhao)孔(kong)(kong)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)平(ping)均(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)≥1.00；陰(yin)性(xing)(xing)(xing)(xing)(xing)對(dui)(dui)(dui)照(zhao)孔(kong)(kong)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)平(ping)均(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)≤0.15，說(shuo)明(ming)(ming)試(shi)驗(yan)(yan)(yan)(yan)結果(guo)(guo)(guo)有(you)(you)(you)效。特(te)異性(xing)(xing)(xing)(xing)(xing)：不與(yu)其它(ta)可溶(rong)性(xing)(xing)(xing)(xing)(xing)結構類似物交叉反(fan)應(ying)。重復性(xing)(xing)(xing)(xing)(xing)：板(ban)內(nei)(nei)、板(ban)間變異系(xi)數均(jun)小(xiao)于(yu)(yu)(yu)(yu)15%。貯藏：2-8℃，避(bi)光防潮(chao)保(bao)存(cun)。有(you)(you)(you)效期(qi)：6個月免責(ze)聲(sheng)明(ming)(ming)試(shi)劑(ji)(ji)(ji)盒(he)僅供(gong)研究(jiu)(jiu)使(shi)(shi)用(yong)(yong)(yong)，不得用(yong)(yong)(yong)于(yu)(yu)(yu)(yu)臨(lin)床實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)或人(ren)體實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)，否(fou)則所(suo)產(chan)生的(de)(de)(de)(de)(de)一切后(hou)(hou)(hou)果(guo)(guo)(guo)，由實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)者承擔，本(ben)公司概(gai)不負(fu)責(ze)。嚴(yan)格(ge)按(an)照(zhao)說(shuo)明(ming)(ming)書(shu)操(cao)作(zuo)(zuo)(zuo)，實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)者違(wei)反(fan)說(shuo)明(ming)(ming)書(shu)操(cao)作(zuo)(zuo)(zuo)，后(hou)(hou)(hou)果(guo)(guo)(guo)由實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)者承擔。技(ji)術(shu)支持電(dian)話：電(dian)子(zi)郵件：網址：fllygs.com生產(chan)廠家：上(shang)(shang)海篤瑪生物科技(ji)有(you)(you)(you)限(xian)公司
人（Human）琥(hu)珀酰(xian)輔(fu)(fu)酶(mei)A（SCOA）ELISA檢測(ce)(ce)(ce)(ce)試(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)本(ben)試(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)只(zhi)能(neng)用(yong)于(yu)(yu)(yu)科學(xue)研(yan)究(jiu)，不(bu)得用(yong)于(yu)(yu)(yu)醫(yi)學(xue)診斷產品(pin)(pin)(pin)(pin)(pin)貨號(hao)：【DM6988】產品(pin)(pin)(pin)(pin)(pin)規(gui)格(ge)：【48T/96T】檢測(ce)(ce)(ce)(ce)范圍：反應(ying)性(xing)(xing)：Human檢測(ce)(ce)(ce)(ce)范圍：3.65-225 nmol/L靈敏(min)度(du)(du)(du)： 1.0 nmol/L檢測(ce)(ce)(ce)(ce)原理(li)試(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)采(cai)用(yong)雙抗體(ti)(ti)(ti)一步(bu)夾心(xin)法(fa)酶(mei)聯(lian)免疫吸(xi)附試(shi)(shi)驗(yan)（ELISA）。往預先包被琥(hu)珀酰(xian)輔(fu)(fu)酶(mei)A（SCOA）抗體(ti)(ti)(ti)的(de)包被微(wei)孔(kong)(kong)中(zhong)，依(yi)次(ci)加(jia)(jia)(jia)入(ru)標(biao)(biao)(biao)本(ben)、標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)記的(de)檢測(ce)(ce)(ce)(ce)抗體(ti)(ti)(ti)，經過(guo)(guo)溫育(yu)并(bing)(bing)(bing)徹底(di)(di)洗滌。用(yong)底(di)(di)物(wu)(wu)(wu)TMB顯(xian)色(se)(se)，TMB在(zai)過(guo)(guo)氧化物(wu)(wu)(wu)酶(mei)的(de)催化下(xia)轉(zhuan)化成(cheng)藍色(se)(se)，并(bing)(bing)(bing)在(zai)酸的(de)作(zuo)(zuo)(zuo)用(yong)下(xia)轉(zhuan)化成(cheng)＊終的(de)黃色(se)(se)。顏色(se)(se)的(de)深淺和(he)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)中(zhong)的(de)琥(hu)珀酰(xian)輔(fu)(fu)酶(mei)A（SCOA）呈正(zheng)相(xiang)關(guan)。用(yong)酶(mei)標(biao)(biao)(biao)儀(yi)在(zai)450nm 波長下(xia)測(ce)(ce)(ce)(ce)定吸(xi)光(guang)度(du)(du)(du)（OD 值），計(ji)算(suan)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)。樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)收集、處理(li)及(ji)(ji)保(bao)(bao)存(cun)方(fang)(fang)法(fa)1. 血(xue)清(qing)：使(shi)(shi)用(yong)不(bu)含熱原和(he)內毒素(su)的(de)試(shi)(shi)管(guan)，操(cao)作(zuo)(zuo)(zuo)過(guo)(guo)程中(zhong)避(bi)免任何細(xi)胞刺激，收集血(xue)液(ye)(ye)后(hou)(hou)(hou)，3000轉(zhuan)離心(xin)10分(fen)鐘(zhong)將(jiang)血(xue)清(qing)和(he)紅細(xi)胞迅速小心(xin)地分(fen)離。2. 血(xue)漿：EDTA、檸檬酸鹽或(huo)肝素(su)抗凝。3000轉(zhuan)離心(xin)30分(fen)鐘(zhong)取上(shang)清(qing)。3. 細(xi)胞上(shang)清(qing)液(ye)(ye)：3000轉(zhuan)離心(xin)10分(fen)鐘(zhong)去除(chu)顆粒和(he)聚合物(wu)(wu)(wu)。4. 組(zu)織勻漿：將(jiang)組(zu)織加(jia)(jia)(jia)入(ru)適(shi)量(liang)生(sheng)理(li)鹽水(shui)搗碎。3000轉(zhuan)離心(xin)10分(fen)鐘(zhong)取上(shang)清(qing)。5. 保(bao)(bao)存(cun)：如(ru)(ru)果(guo)樣(yang)(yang)(yang)本(ben)收集后(hou)(hou)(hou)不(bu)及(ji)(ji)時(shi)檢測(ce)(ce)(ce)(ce)，請(qing)按(an)(an)一次(ci)用(yong)量(liang)分(fen)裝，凍存(cun)于(yu)(yu)(yu)-20℃，避(bi)免反復凍融，在(zai)室(shi)溫下(xia)解(jie)凍并(bing)(bing)(bing)確保(bao)(bao)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)均勻地充(chong)分(fen)解(jie)凍。自備物(wu)(wu)(wu)品(pin)(pin)(pin)(pin)(pin)酶(mei)標(biao)(biao)(biao)儀(yi)（450nm）高精度(du)(du)(du)加(jia)(jia)(jia)樣(yang)(yang)(yang)器及(ji)(ji)槍(qiang)頭：0.5-10uL、2-20uL、20-200uL、200-1000uL37℃恒溫箱(xiang)操(cao)作(zuo)(zuo)(zuo)注意事項試(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)保(bao)(bao)存(cun)在(zai)2-8℃，使(shi)(shi)用(yong)前室(shi)溫平衡20分(fen)鐘(zhong)。從冰箱(xiang)取出(chu)的(de)濃(nong)(nong)縮(suo)洗滌液(ye)(ye)會(hui)有(you)結(jie)(jie)晶(jing)，這(zhe)屬(shu)于(yu)(yu)(yu)正(zheng)常現象，水(shui)浴(yu)加(jia)(jia)(jia)熱使(shi)(shi)結(jie)(jie)晶(jing)完全溶(rong)解(jie)后(hou)(hou)(hou)再使(shi)(shi)用(yong)。實(shi)(shi)驗(yan)中(zhong)不(bu)用(yong)的(de)板(ban)(ban)(ban)條應(ying)立即(ji)放回(hui)(hui)自封(feng)(feng)(feng)袋中(zhong)，密(mi)封(feng)(feng)(feng)（低溫干燥）保(bao)(bao)存(cun)。濃(nong)(nong)度(du)(du)(du)為(wei)0的(de)S0號(hao)標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)即(ji)可(ke)(ke)視為(wei)陰性(xing)(xing)對(dui)照(zhao)或(huo)者空白(bai)；按(an)(an)照(zhao)說(shuo)明書(shu)操(cao)作(zuo)(zuo)(zuo)時(shi)樣(yang)(yang)(yang)本(ben)已經稀釋5倍，＊終結(jie)(jie)果(guo)乘以5才是(shi)樣(yang)(yang)(yang)本(ben)實(shi)(shi)際濃(nong)(nong)度(du)(du)(du)。嚴格(ge)按(an)(an)照(zhao)說(shuo)明書(shu)中(zhong)標(biao)(biao)(biao)明的(de)時(shi)間、加(jia)(jia)(jia)液(ye)(ye)量(liang)及(ji)(ji)順序進行溫育(yu)操(cao)作(zuo)(zuo)(zuo)。所有(you)液(ye)(ye)體(ti)(ti)(ti)組(zu)分(fen)使(shi)(shi)用(yong)前充(chong)分(fen)搖(yao)勻。試(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)組(zu)成(cheng)名稱96孔(kong)(kong)配置(zhi)(zhi)48孔(kong)(kong)配置(zhi)(zhi)備注微(wei)孔(kong)(kong)酶(mei)標(biao)(biao)(biao)板(ban)(ban)(ban)12孔(kong)(kong)×8條12孔(kong)(kong)×4條無(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)0.3mL*6管(guan)0.3mL*6管(guan)無(wu)(wu)(wu)(wu)(wu)(wu)樣(yang)(yang)(yang)本(ben)稀釋液(ye)(ye)6mL3mL無(wu)(wu)(wu)(wu)(wu)(wu)檢測(ce)(ce)(ce)(ce)抗體(ti)(ti)(ti)-HRP10mL5mL無(wu)(wu)(wu)(wu)(wu)(wu)20×洗滌緩(huan)沖(chong)液(ye)(ye)25mL15mL按(an)(an)說(shuo)明書(shu)進行稀釋底(di)(di)物(wu)(wu)(wu)A6mL3mL無(wu)(wu)(wu)(wu)(wu)(wu)底(di)(di)物(wu)(wu)(wu)B6mL3mL無(wu)(wu)(wu)(wu)(wu)(wu)終止(zhi)液(ye)(ye)6mL3mL無(wu)(wu)(wu)(wu)(wu)(wu)封(feng)(feng)(feng)板(ban)(ban)(ban)膜(mo)2張(zhang)(zhang)2張(zhang)(zhang)無(wu)(wu)(wu)(wu)(wu)(wu)說(shuo)明書(shu)1份1份無(wu)(wu)(wu)(wu)(wu)(wu)自封(feng)(feng)(feng)袋1個1個無(wu)(wu)(wu)(wu)(wu)(wu)注：標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)（S0-S5）濃(nong)(nong)度(du)(du)(du)依(yi)次(ci)為(wei)：0、7.5、15、30、60、120 nmol/L試(shi)(shi)劑(ji)(ji)的(de)準(zhun)(zhun)備20×洗滌緩(huan)沖(chong)液(ye)(ye)的(de)稀釋：蒸(zheng)餾水(shui)按(an)(an)1：20稀釋，即(ji)1份的(de)20×洗滌緩(huan)沖(chong)液(ye)(ye)加(jia)(jia)(jia)19份的(de)蒸(zheng)餾水(shui)。洗板(ban)(ban)(ban)方(fang)(fang)法(fa)手工洗板(ban)(ban)(ban)：甩盡(jin)孔(kong)(kong)內液(ye)(ye)體(ti)(ti)(ti)，每孔(kong)(kong)加(jia)(jia)(jia)滿洗滌液(ye)(ye)，靜置(zhi)(zhi)1min后(hou)(hou)(hou)甩盡(jin)孔(kong)(kong)內液(ye)(ye)體(ti)(ti)(ti)，在(zai)吸(xi)水(shui)紙上(shang)拍干，如(ru)(ru)此(ci)洗板(ban)(ban)(ban)5次(ci)。自動(dong)洗板(ban)(ban)(ban)機：每孔(kong)(kong)注入(ru)洗液(ye)(ye)350μL，浸泡1min，洗板(ban)(ban)(ban)5次(ci)。操(cao)作(zuo)(zuo)(zuo)步(bu)驟 結(jie)(jie)果(guo)判斷繪制標(biao)(biao)(biao)準(zhun)(zhun)曲線(xian)：在(zai)Excel工作(zuo)(zuo)(zuo)表中(zhong)，以標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)作(zuo)(zuo)(zuo)橫坐標(biao)(biao)(biao)，對(dui)應(ying)OD值作(zuo)(zuo)(zuo)縱坐標(biao)(biao)(biao)，繪制出(chu)標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)線(xian)性(xing)(xing)回(hui)(hui)歸(gui)曲線(xian)，按(an)(an)曲線(xian)方(fang)(fang)程計(ji)算(suan)各樣(yang)(yang)(yang)本(ben)濃(nong)(nong)度(du)(du)(du)值。注：此(ci)圖片(pian)僅(jin)供(gong)參(can)考試(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)性(xing)(xing)能(neng)準(zhun)(zhun)確性(xing)(xing)：標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)線(xian)性(xing)(xing)回(hui)(hui)歸(gui)與(yu)預期濃(nong)(nong)度(du)(du)(du)相(xiang)關(guan)系數R值，大于(yu)(yu)(yu)等(deng)于(yu)(yu)(yu)0.9900。靈敏(min)度(du)(du)(du)：＊低檢測(ce)(ce)(ce)(ce)濃(nong)(nong)度(du)(du)(du)小于(yu)(yu)(yu)1.0 ng/mL。特異(yi)性(xing)(xing)：不(bu)與(yu)其它可(ke)(ke)溶(rong)性(xing)(xing)結(jie)(jie)構類(lei)似(si)物(wu)(wu)(wu)交叉反應(ying)。重復性(xing)(xing)：板(ban)(ban)(ban)內、板(ban)(ban)(ban)間變(bian)異(yi)系數均小于(yu)(yu)(yu)15%。貯藏：2-8℃，避(bi)光(guang)防潮保(bao)(bao)存(cun)。有(you)效期：6個月(yue)免責聲明試(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)僅(jin)供(gong)研(yan)究(jiu)使(shi)(shi)用(yong)，不(bu)得用(yong)于(yu)(yu)(yu)臨(lin)床(chuang)實(shi)(shi)驗(yan)或(huo)人體(ti)(ti)(ti)實(shi)(shi)驗(yan)，否則所產生(sheng)的(de)一切后(hou)(hou)(hou)果(guo)，由實(shi)(shi)驗(yan)者承擔，本(ben)公司(si)概(gai)不(bu)負責。嚴格(ge)按(an)(an)照(zhao)說(shuo)明書(shu)操(cao)作(zuo)(zuo)(zuo)，實(shi)(shi)驗(yan)者違反說(shuo)明書(shu)操(cao)作(zuo)(zuo)(zuo)，后(hou)(hou)(hou)果(guo)由實(shi)(shi)驗(yan)者承擔。技術支持電話：電子郵件：網址：fllygs.com生(sheng)產廠家：上(shang)海篤瑪(ma)生(sheng)物(wu)(wu)(wu)科技有(you)限公司(si)
上(shang)(shang)(shang)(shang)海篤瑪(ma)生(sheng)物(wu)(wu)(wu)科技有(you)(you)限公司人（Human）硫(liu)酸(suan)酯酶(mei)(mei)2（SULF2） ELISA檢(jian)(jian)測(ce)(ce)(ce)試(shi)(shi)(shi)劑盒(he)(he)本(ben)(ben)(ben)試(shi)(shi)(shi)劑盒(he)(he)只(zhi)能用(yong)(yong)于(yu)科學研究，不(bu)(bu)(bu)得(de)用(yong)(yong)于(yu)醫學診斷(duan)商品(pin)(pin)(pin)(pin)(pin)(pin)貨號：DM6987產品(pin)(pin)(pin)(pin)(pin)(pin)規(gui)格(ge)：48T/96T檢(jian)(jian)測(ce)(ce)(ce)范圍：反(fan)(fan)應(ying)性(xing)(xing)(xing)：Human檢(jian)(jian)測(ce)(ce)(ce)范圍：3.65-225U/ml靈敏度(du)(du)(du)(du)： 1.0 U/ml檢(jian)(jian)測(ce)(ce)(ce)原理：試(shi)(shi)(shi)劑盒(he)(he)采用(yong)(yong)雙抗(kang)(kang)體(ti)(ti)(ti)(ti)一步夾(jia)心(xin)(xin)法酶(mei)(mei)聯免(mian)疫吸(xi)附試(shi)(shi)(shi)驗(yan)（ELISA）。往 預(yu)先包被硫(liu)酸(suan)酯酶(mei)(mei)2（SULF2）抗(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)包被微(wei)孔(kong)(kong)(kong)(kong)中(zhong)，依(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)入(ru)標(biao)(biao)(biao)本(ben)(ben)(ben)、 標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、 HRP標(biao)(biao)(biao)記(ji)的(de)(de)(de)(de)檢(jian)(jian)測(ce)(ce)(ce)抗(kang)(kang)體(ti)(ti)(ti)(ti)，經(jing)過(guo)(guo)溫育(yu)并(bing)徹(che)底洗(xi)(xi)(xi)(xi)滌(di)(di)。用(yong)(yong)底物(wu)(wu)(wu)TMB 顯色(se)，TMB在(zai)(zai)過(guo)(guo)氧(yang)化(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)的(de)(de)(de)(de)催化(hua)(hua)下(xia)轉(zhuan)化(hua)(hua)成(cheng)(cheng)藍色(se)，并(bing)在(zai)(zai)酸(suan)的(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)下(xia)轉(zhuan) 化(hua)(hua)成(cheng)(cheng)＊終的(de)(de)(de)(de)黃色(se)。顏色(se)的(de)(de)(de)(de)深淺(qian)和(he)樣(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)硫(liu)酸(suan)酯酶(mei)(mei)2（SULF2）呈正 相關。用(yong)(yong)酶(mei)(mei)標(biao)(biao)(biao)儀在(zai)(zai)450nm 波長下(xia)測(ce)(ce)(ce)定吸(xi)光(guang)(guang)度(du)(du)(du)(du)（OD 值），計算樣(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin) 活性(xing)(xing)(xing)。樣(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)收(shou)(shou)集、存(cun)(cun)儲及(ji)保(bao)存(cun)(cun)方法：1. 血清(qing)：使(shi)(shi)用(yong)(yong)不(bu)(bu)(bu)含熱原和(he)內毒素的(de)(de)(de)(de)試(shi)(shi)(shi)管，操(cao)(cao)作(zuo)(zuo)過(guo)(guo)程中(zhong)避免(mian)任何細(xi)胞(bao) 刺(ci)激(ji)，收(shou)(shou)集血液(ye)(ye)(ye)(ye)后(hou)(hou)，3000 轉(zhuan)離(li)(li)心(xin)(xin) 10 分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)將血清(qing)和(he)紅(hong)細(xi)胞(bao)迅速小(xiao)心(xin)(xin) 地分(fen)(fen)(fen)(fen)離(li)(li)。2. 血漿：EDTA、檸(ning)檬酸(suan)鹽或肝素抗(kang)(kang)凝。3000 轉(zhuan)離(li)(li)心(xin)(xin) 30 分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)取(qu)上(shang)(shang)(shang)(shang) 清(qing)。3. 細(xi)胞(bao)上(shang)(shang)(shang)(shang)清(qing)液(ye)(ye)(ye)(ye)：3000 轉(zhuan)離(li)(li)心(xin)(xin) 10 分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)去(qu)除(chu)顆(ke)粒和(he)聚合物(wu)(wu)(wu)。4. 組織勻(yun)漿：將組織加(jia)(jia)(jia)(jia)入(ru)適量生(sheng)理鹽水(shui)(shui)搗碎。 3000 轉(zhuan)離(li)(li)心(xin)(xin) 10 分(fen)(fen)(fen)(fen) 鐘(zhong)(zhong)取(qu)上(shang)(shang)(shang)(shang)清(qing)。5. 保(bao)存(cun)(cun)：如果(guo)(guo)樣(yang)(yang)本(ben)(ben)(ben)收(shou)(shou)集后(hou)(hou)不(bu)(bu)(bu)及(ji)時(shi)檢(jian)(jian)測(ce)(ce)(ce)，請按(an)(an)一次(ci)(ci)用(yong)(yong)量分(fen)(fen)(fen)(fen)裝，凍存(cun)(cun)于(yu) -20℃ , 避免(mian)反(fan)(fan)復(fu)凍融，在(zai)(zai)室(shi)溫下(xia)解(jie)凍并(bing)確保(bao)樣(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)均勻(yun)地充分(fen)(fen)(fen)(fen)解(jie)凍。自備(bei)(bei)物(wu)(wu)(wu)品(pin)(pin)(pin)(pin)(pin)(pin)：1. 酶(mei)(mei)標(biao)(biao)(biao)儀（450nm）2. 高 精 度(du)(du)(du)(du) 加(jia)(jia)(jia)(jia) 樣(yang)(yang) 器 及(ji) 槍(qiang) 頭 ： 0.5-10uL 、 2-20uL 、 20-200uL 、200-1000uL3. 37℃恒溫箱操(cao)(cao)作(zuo)(zuo)注(zhu)意事項(xiang)：1. 試(shi)(shi)(shi)劑盒(he)(he)保(bao)存(cun)(cun)在(zai)(zai) 2-8℃ , 使(shi)(shi)用(yong)(yong)前室(shi)溫平衡(heng) 20 分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。從(cong)冰(bing)箱取(qu)出(chu)的(de)(de)(de)(de)濃(nong)(nong)(nong) 縮洗(xi)(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)會有(you)(you)結晶(jing)，這屬于(yu)正常現(xian)象，水(shui)(shui)浴加(jia)(jia)(jia)(jia)熱使(shi)(shi)結晶(jing)完(wan)全(quan)溶解(jie)后(hou)(hou) 再使(shi)(shi)用(yong)(yong)。2. 實(shi)(shi)(shi)驗(yan)中(zhong)不(bu)(bu)(bu)用(yong)(yong)的(de)(de)(de)(de)板(ban)(ban)(ban)(ban)(ban)條應(ying)立(li)即(ji)放(fang)回自封(feng)袋中(zhong)，密封(feng)（低溫干(gan)燥）保(bao)存(cun)(cun)。 3. 濃(nong)(nong)(nong)度(du)(du)(du)(du)為(wei) 0 的(de)(de)(de)(de) S0 號標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)即(ji)可(ke)(ke)視為(wei)陰性(xing)(xing)(xing)對照(zhao)(zhao)或者(zhe)空(kong)白；按(an)(an)照(zhao)(zhao)說(shuo)明(ming)(ming)(ming) 書(shu)(shu)操(cao)(cao)作(zuo)(zuo)時(shi)樣(yang)(yang)本(ben)(ben)(ben)已經(jing)稀(xi)(xi)釋(shi) 5 倍，＊終結果(guo)(guo)乘以 5 才是樣(yang)(yang)本(ben)(ben)(ben)實(shi)(shi)(shi)際濃(nong)(nong)(nong)度(du)(du)(du)(du)。4. 嚴格(ge)按(an)(an)照(zhao)(zhao)說(shuo)明(ming)(ming)(ming)書(shu)(shu)中(zhong)標(biao)(biao)(biao)明(ming)(ming)(ming)的(de)(de)(de)(de)時(shi)間、加(jia)(jia)(jia)(jia)液(ye)(ye)(ye)(ye)量及(ji)順序進(jin)行(xing)溫育(yu)操(cao)(cao)作(zuo)(zuo)。5. 所有(you)(you)液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)組分(fen)(fen)(fen)(fen)使(shi)(shi)用(yong)(yong)前充分(fen)(fen)(fen)(fen)搖勻(yun)。試(shi)(shi)(shi)劑盒(he)(he)組成(cheng)(cheng)：名(ming)稱(cheng)96 孔(kong)(kong)(kong)(kong)配置(zhi)48 孔(kong)(kong)(kong)(kong)配置(zhi)備(bei)(bei)注(zhu)微(wei)孔(kong)(kong)(kong)(kong)酶(mei)(mei)標(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)12 孔(kong)(kong)(kong)(kong)×8 條12 孔(kong)(kong)(kong)(kong) ×4 條無標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6 管0.3mL*6 管無樣(yang)(yang)本(ben)(ben)(ben)稀(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)6mL3mL無檢(jian)(jian)測(ce)(ce)(ce)抗(kang)(kang)體(ti)(ti)(ti)(ti)-HRP10mL5mL無20 ×洗(xi)(xi)(xi)(xi)滌(di)(di)緩沖(chong)液(ye)(ye)(ye)(ye)25mL15mL按(an)(an)說(shuo)明(ming)(ming)(ming)書(shu)(shu)進(jin)行(xing)稀(xi)(xi)釋(shi)底物(wu)(wu)(wu) A6mL3mL無底物(wu)(wu)(wu) B6mL3mL無終止液(ye)(ye)(ye)(ye)6mL3mL無封(feng)板(ban)(ban)(ban)(ban)(ban)膜2 張2 張無說(shuo)明(ming)(ming)(ming)書(shu)(shu)1 份(fen)1 份(fen)無自封(feng)袋1 個(ge)1 個(ge)無注(zhu)：標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)（ S0-S5）濃(nong)(nong)(nong)度(du)(du)(du)(du)依(yi)次(ci)(ci)為(wei)：0、 12.5、25、 50、 100、200 ng/mL試(shi)(shi)(shi)劑的(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)備(bei)(bei)：20×洗(xi)(xi)(xi)(xi)滌(di)(di)緩沖(chong)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)稀(xi)(xi)釋(shi)：蒸餾水(shui)(shui)按(an)(an) 1：20 稀(xi)(xi)釋(shi)，即(ji) 1 份(fen)的(de)(de)(de)(de) 20×洗(xi)(xi)(xi)(xi)滌(di)(di) 緩沖(chong)液(ye)(ye)(ye)(ye)加(jia)(jia)(jia)(jia) 19 份(fen)的(de)(de)(de)(de)蒸餾水(shui)(shui)。洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)方法：1. 手工洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)：甩(shuai)(shuai)盡孔(kong)(kong)(kong)(kong)內液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，每(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)，靜置(zhi) 1min 后(hou)(hou)甩(shuai)(shuai)盡 孔(kong)(kong)(kong)(kong)內液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，在(zai)(zai)吸(xi)水(shui)(shui)紙上(shang)(shang)(shang)(shang)拍干(gan)，如此(ci)(ci)(ci)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban) 5 次(ci)(ci)。2. 自動洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)機：每(mei)(mei)孔(kong)(kong)(kong)(kong)注(zhu)入(ru)洗(xi)(xi)(xi)(xi)液(ye)(ye)(ye)(ye) 350μL，浸(jin)泡 1min，洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban) 5 次(ci)(ci)。結果(guo)(guo)判斷(duan)：繪(hui)制標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線：在(zai)(zai) Excel 工作(zuo)(zuo)表中(zhong)，以標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)作(zuo)(zuo)橫坐標(biao)(biao)(biao)，對應(ying) OD 值作(zuo)(zuo)縱坐標(biao)(biao)(biao)，繪(hui)制出(chu)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)線性(xing)(xing)(xing)回歸(gui)曲(qu)線，按(an)(an)曲(qu)線方程計算各樣(yang)(yang) 本(ben)(ben)(ben)濃(nong)(nong)(nong)度(du)(du)(du)(du)值。注(zhu)：此(ci)(ci)(ci)圖(tu)片僅(jin)供(gong)參考試(shi)(shi)(shi)劑盒(he)(he)性(xing)(xing)(xing)能：1. 準(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)(xing)：標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)線性(xing)(xing)(xing)回歸(gui)與(yu)預(yu)期濃(nong)(nong)(nong)度(du)(du)(du)(du)相關系數 R 值，大于(yu)等于(yu) 0.9900。2. 靈敏度(du)(du)(du)(du)：＊低檢(jian)(jian)測(ce)(ce)(ce)濃(nong)(nong)(nong)度(du)(du)(du)(du)小(xiao)于(yu) 1.0 ng/mL。3. 特異性(xing)(xing)(xing)：不(bu)(bu)(bu)與(yu)其它可(ke)(ke)溶性(xing)(xing)(xing)結構類似物(wu)(wu)(wu)交叉反(fan)(fan)應(ying)。4. 重(zhong)復(fu)性(xing)(xing)(xing)：板(ban)(ban)(ban)(ban)(ban)內、板(ban)(ban)(ban)(ban)(ban)間變異系數均小(xiao)于(yu) 15%。5. 貯藏(zang)：2-8℃ , 避光(guang)(guang)防潮保(bao)存(cun)(cun)。6. 有(you)(you)效期：6 個(ge)月操(cao)(cao)作(zuo)(zuo)步驟：1. 從(cong)室(shi)溫平衡(heng)20min后(hou)(hou)的(de)(de)(de)(de)鋁箔袋中(zhong)取(qu)出(chu)所需板(ban)(ban)(ban)(ban)(ban)條，剩余板(ban)(ban)(ban)(ban)(ban)條用(yong)(yong)自封(feng)袋密封(feng)放(fang)回4℃。2. 設(she)置(zhi)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)和(he)樣(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)，標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)各加(jia)(jia)(jia)(jia)不(bu)(bu)(bu)同濃(nong)(nong)(nong)度(du)(du)(du)(du)的(de)(de)(de)(de)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)50μL；3. 樣(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)待(dai)測(ce)(ce)(ce)樣(yang)(yang)本(ben)(ben)(ben)10μL，再加(jia)(jia)(jia)(jia)樣(yang)(yang)本(ben)(ben)(ben)稀(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)40μL；空(kong)白孔(kong)(kong)(kong)(kong)不(bu)(bu)(bu)加(jia)(jia)(jia)(jia)。4. 除(chu)空(kong)白孔(kong)(kong)(kong)(kong)外，標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)和(he)樣(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)中(zhong)每(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)辣根過(guo)(guo)氧(yang)化(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)（HRP）標(biao)(biao)(biao)記(ji)的(de)(de)(de)(de)檢(jian)(jian)測(ce)(ce)(ce)抗(kang)(kang)體(ti)(ti)(ti)(ti)100μL，用(yong)(yong)封(feng)板(ban)(ban)(ban)(ban)(ban)膜封(feng)住反(fan)(fan)應(ying)孔(kong)(kong)(kong)(kong)，37℃水(shui)(shui)浴鍋(guo)或恒溫箱溫育(yu)60min。5. 棄(qi)去(qu)液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，吸(xi)水(shui)(shui)紙上(shang)(shang)(shang)(shang)拍干(gan)，每(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)，靜置(zhi)1min，甩(shuai)(shuai)去(qu)洗(xi)(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)，吸(xi)水(shui)(shui)紙上(shang)(shang)(shang)(shang)拍干(gan)，如此(ci)(ci)(ci)重(zhong)復(fu)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)5次(ci)(ci)（也可(ke)(ke)用(yong)(yong)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)機洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)）。6. 每(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)底物(wu)(wu)(wu)A、B各50μL，37℃避光(guang)(guang)孵(fu)育(yu)15min。7. 每(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)終止液(ye)(ye)(ye)(ye)50μL，15min內，在(zai)(zai)450nm波長處測(ce)(ce)(ce)定各孔(kong)(kong)(kong)(kong)的(de)(de)(de)(de)OD值。技術支持：電(dian)(dian)話(hua)：電(dian)(dian)子(zi)郵件：網址(zhi)：fllygs.com生(sheng)產廠家：上(shang)(shang)(shang)(shang)海篤瑪(ma)生(sheng)物(wu)(wu)(wu)科技有(you)(you)限公司免(mian)責聲明(ming)(ming)(ming)：1. 試(shi)(shi)(shi)劑盒(he)(he)僅(jin)供(gong)研究使(shi)(shi)用(yong)(yong)，不(bu)(bu)(bu)得(de)用(yong)(yong)于(yu)臨(lin)床實(shi)(shi)(shi)驗(yan)或人體(ti)(ti)(ti)(ti)實(shi)(shi)(shi)驗(yan)，否則所 產生(sheng)的(de)(de)(de)(de)一切后(hou)(hou)果(guo)(guo)，由實(shi)(shi)(shi)驗(yan)者(zhe)承擔(dan)，本(ben)(ben)(ben)公司概(gai)不(bu)(bu)(bu)負(fu)責。2. 嚴格(ge)按(an)(an)照(zhao)(zhao)說(shuo)明(ming)(ming)(ming)書(shu)(shu)操(cao)(cao)作(zuo)(zuo)，實(shi)(shi)(shi)驗(yan)者(zhe)違反(fan)(fan)說(shuo)明(ming)(ming)(ming)書(shu)(shu)操(cao)(cao)作(zuo)(zuo)，后(hou)(hou)果(guo)(guo)由實(shi)(shi)(shi)驗(yan)者(zhe) 承擔(dan)
上(shang)海(hai)篤(du)瑪生(sheng)(sheng)物(wu)(wu)科技(ji)有(you)(you)限公司(si)人跨膜(mo)蛋(dan)白 212ELISA 檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)劑(ji)盒Human Transmembrane Protein 212 ELISA Detection Kit本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)盒只(zhi)能(neng)用于(yu)科學研究，不(bu)(bu)得用于(yu)醫(yi)學診斷(duan)商品(pin)貨號：DM6986產品(pin)規格(ge)：48T/96T檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)范圍(wei)：反(fan)應(ying)(ying)性：Human檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)范圍(wei)：17.45-886 ng/mL 靈(ling)敏度(du)(du)(du)(du)： 1.0 U/ml檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)原理(li)：試(shi)(shi)(shi)(shi)劑(ji)盒采(cai)用雙(shuang)抗(kang)(kang)體(ti)(ti)一(yi)步(bu)夾心(xin)(xin)法酶(mei)(mei)(mei)聯免疫吸附(fu)試(shi)(shi)(shi)(shi)驗（ELISA）。往(wang) 預先包(bao)被(bei)跨膜(mo)蛋(dan)白212（TMEM212）抗(kang)(kang)體(ti)(ti)的(de)(de)(de)包(bao)被(bei)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，依次(ci)(ci)加(jia)入(ru) 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)、 HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記的(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)抗(kang)(kang)體(ti)(ti)，經(jing)過(guo)(guo)溫(wen)(wen)(wen)育(yu)并(bing)徹(che)底洗滌(di)。用底 物(wu)(wu)TMB顯色，TMB在(zai)過(guo)(guo)氧化物(wu)(wu)酶(mei)(mei)(mei)的(de)(de)(de)催(cui)化下轉(zhuan)化成藍色，并(bing)在(zai)酸的(de)(de)(de)作(zuo) 用下轉(zhuan)化成＊終(zhong)的(de)(de)(de)黃(huang)色 。 顏(yan)色的(de)(de)(de)深淺(qian)和(he)(he)(he)樣(yang)(yang)品(pin)中(zhong)的(de)(de)(de)跨膜(mo)蛋(dan)白212 （ TMEM212 ）呈正(zheng)(zheng)相關(guan)(guan)。用酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)450nm 波(bo)長下測(ce)(ce)(ce)(ce)定吸光度(du)(du)(du)(du) （ OD 值(zhi)），計算樣(yang)(yang)品(pin)濃(nong)度(du)(du)(du)(du)。樣(yang)(yang)品(pin)收集(ji)、存(cun)(cun)儲及保(bao)存(cun)(cun)方法：1. 血(xue)清：使用不(bu)(bu)含熱原和(he)(he)(he)內(nei)毒(du)素(su)的(de)(de)(de)試(shi)(shi)(shi)(shi)管，操(cao)作(zuo)過(guo)(guo)程(cheng)中(zhong)避(bi)免任何細胞(bao)(bao) 刺激，收集(ji)血(xue)液(ye)(ye)(ye)(ye)(ye)后(hou)(hou)，3000 轉(zhuan)離(li)心(xin)(xin) 10 分(fen)(fen)鐘將(jiang)(jiang)血(xue)清和(he)(he)(he)紅細胞(bao)(bao)迅(xun)速小心(xin)(xin) 地分(fen)(fen)離(li)。2. 血(xue)漿：EDTA、檸檬酸鹽(yan)或肝素(su)抗(kang)(kang)凝。3000 轉(zhuan)離(li)心(xin)(xin) 30 分(fen)(fen)鐘取上(shang) 清。3. 細胞(bao)(bao)上(shang)清液(ye)(ye)(ye)(ye)(ye)：3000 轉(zhuan)離(li)心(xin)(xin) 10 分(fen)(fen)鐘去(qu)除顆粒和(he)(he)(he)聚合物(wu)(wu)。4. 組織(zhi)勻漿：將(jiang)(jiang)組織(zhi)加(jia)入(ru)適量(liang)(liang)生(sheng)(sheng)理(li)鹽(yan)水(shui)搗碎。 3000 轉(zhuan)離(li)心(xin)(xin) 10 分(fen)(fen) 鐘取上(shang)清。5. 保(bao)存(cun)(cun)：如(ru)果樣(yang)(yang)本(ben)(ben)(ben)收集(ji)后(hou)(hou)不(bu)(bu)及時檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)，請按一(yi)次(ci)(ci)用量(liang)(liang)分(fen)(fen)裝，凍存(cun)(cun)于(yu) -20℃ , 避(bi)免反(fan)復(fu)凍融，在(zai)室(shi)(shi)溫(wen)(wen)(wen)下解(jie)(jie)凍并(bing)確(que)保(bao)樣(yang)(yang)品(pin)均勻地充分(fen)(fen)解(jie)(jie)凍。自(zi)(zi)備物(wu)(wu)品(pin)：1. 酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀（450nm）2. 高 精 度(du)(du)(du)(du) 加(jia) 樣(yang)(yang) 器 及 槍 頭 ： 0.5-10uL 、 2-20uL 、 20-200uL 、200-1000uL3. 37℃恒溫(wen)(wen)(wen)箱操(cao)作(zuo)注(zhu)(zhu)意事項：1. 試(shi)(shi)(shi)(shi)劑(ji)盒保(bao)存(cun)(cun)在(zai) 2-8℃ , 使用前(qian)室(shi)(shi)溫(wen)(wen)(wen)平衡 20 分(fen)(fen)鐘。從冰箱取出(chu)的(de)(de)(de)濃(nong) 縮洗滌(di)液(ye)(ye)(ye)(ye)(ye)會有(you)(you)結晶(jing)，這(zhe)屬于(yu)正(zheng)(zheng)常現象，水(shui)浴加(jia)熱使結晶(jing)完(wan)全(quan)溶(rong)解(jie)(jie)后(hou)(hou) 再使用。2. 實(shi)(shi)驗中(zhong)不(bu)(bu)用的(de)(de)(de)板(ban)(ban)條(tiao)應(ying)(ying)立即(ji)放(fang)回(hui)自(zi)(zi)封(feng)袋(dai)中(zhong)，密(mi)封(feng)（低(di)溫(wen)(wen)(wen)干(gan)(gan)燥）保(bao)存(cun)(cun)。 3. 濃(nong)度(du)(du)(du)(du)為 0 的(de)(de)(de) S0 號標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)即(ji)可視為陰(yin)性對照(zhao)(zhao)或者(zhe)空白；按照(zhao)(zhao)說(shuo)明 書(shu)操(cao)作(zuo)時樣(yang)(yang)本(ben)(ben)(ben)已經(jing)稀(xi)釋 5 倍，＊終(zhong)結果乘以 5 才(cai)是樣(yang)(yang)本(ben)(ben)(ben)實(shi)(shi)際濃(nong)度(du)(du)(du)(du)。4. 嚴格(ge)按照(zhao)(zhao)說(shuo)明書(shu)中(zhong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)明的(de)(de)(de)時間、加(jia)液(ye)(ye)(ye)(ye)(ye)量(liang)(liang)及順序(xu)進(jin)行(xing)溫(wen)(wen)(wen)育(yu)操(cao)作(zuo)。5. 所有(you)(you)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)組分(fen)(fen)使用前(qian)充分(fen)(fen)搖(yao)勻。試(shi)(shi)(shi)(shi)劑(ji)盒組成：名稱96 孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)(pei)置(zhi)48 孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)(pei)置(zhi)備注(zhu)(zhu)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)12 孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8 條(tiao)12 孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong) ×4 條(tiao)無(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)0.3mL*6 管0.3mL*6 管無(wu)(wu)(wu)樣(yang)(yang)本(ben)(ben)(ben)稀(xi)釋液(ye)(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)(wu)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)抗(kang)(kang)體(ti)(ti)-HRP10mL5mL無(wu)(wu)(wu)20 ×洗滌(di)緩(huan)沖液(ye)(ye)(ye)(ye)(ye)25mL15mL按說(shuo)明書(shu)進(jin)行(xing)稀(xi)釋底物(wu)(wu) A6mL3mL無(wu)(wu)(wu)底物(wu)(wu) B6mL3mL無(wu)(wu)(wu)終(zhong)止液(ye)(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)(wu)封(feng)板(ban)(ban)膜(mo)2 張(zhang)2 張(zhang)無(wu)(wu)(wu)說(shuo)明書(shu)1 份(fen)(fen)1 份(fen)(fen)無(wu)(wu)(wu)自(zi)(zi)封(feng)袋(dai)1 個1 個無(wu)(wu)(wu)注(zhu)(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)（ S0-S5）濃(nong)度(du)(du)(du)(du)依次(ci)(ci)為：0、 12.5、25、 50、 100、200 ng/mL試(shi)(shi)(shi)(shi)劑(ji)的(de)(de)(de)準(zhun)備：20×洗滌(di)緩(huan)沖液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)稀(xi)釋：蒸(zheng)餾水(shui)按 1：20 稀(xi)釋，即(ji) 1 份(fen)(fen)的(de)(de)(de) 20×洗滌(di) 緩(huan)沖液(ye)(ye)(ye)(ye)(ye)加(jia) 19 份(fen)(fen)的(de)(de)(de)蒸(zheng)餾水(shui)。洗板(ban)(ban)方法：1. 手工洗板(ban)(ban)：甩(shuai)盡孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)滿(man)洗滌(di)液(ye)(ye)(ye)(ye)(ye)，靜置(zhi) 1min 后(hou)(hou)甩(shuai)盡 孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，在(zai)吸水(shui)紙上(shang)拍(pai)干(gan)(gan)，如(ru)此洗板(ban)(ban) 5 次(ci)(ci)。2. 自(zi)(zi)動洗板(ban)(ban)機：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)注(zhu)(zhu)入(ru)洗液(ye)(ye)(ye)(ye)(ye) 350μL，浸泡(pao) 1min，洗板(ban)(ban) 5 次(ci)(ci)。結果判斷(duan)：繪制(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)曲線：在(zai) Excel 工作(zuo)表中(zhong)，以標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)濃(nong)度(du)(du)(du)(du)作(zuo)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，對應(ying)(ying) OD 值(zhi)作(zuo)縱(zong)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，繪制(zhi)(zhi)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)線性回(hui)歸曲線，按曲線方程(cheng)計算各(ge)(ge)樣(yang)(yang) 本(ben)(ben)(ben)濃(nong)度(du)(du)(du)(du)值(zhi)。注(zhu)(zhu)：此圖片僅供參(can)考試(shi)(shi)(shi)(shi)劑(ji)盒性能(neng)：1. 準(zhun)確(que)性：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)線性回(hui)歸與預期濃(nong)度(du)(du)(du)(du)相關(guan)(guan)系(xi)數 R 值(zhi)，大于(yu)等于(yu) 0.9900。2. 靈(ling)敏度(du)(du)(du)(du)：＊低(di)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)濃(nong)度(du)(du)(du)(du)小于(yu) 1.0 ng/mL。3. 特異性：不(bu)(bu)與其它可溶(rong)性結構類似(si)物(wu)(wu)交(jiao)叉反(fan)應(ying)(ying)。4. 重(zhong)復(fu)性：板(ban)(ban)內(nei)、板(ban)(ban)間變異系(xi)數均小于(yu) 15%。5. 貯藏：2-8℃ , 避(bi)光防潮保(bao)存(cun)(cun)。6. 有(you)(you)效期：6 個月操(cao)作(zuo)步(bu)驟(zou)：1. 從室(shi)(shi)溫(wen)(wen)(wen)平衡20min后(hou)(hou)的(de)(de)(de)鋁箔(bo)袋(dai)中(zhong)取出(chu)所需(xu)板(ban)(ban)條(tiao)，剩余板(ban)(ban)條(tiao)用自(zi)(zi)封(feng)袋(dai)密(mi)封(feng)放(fang)回(hui)4℃。2. 設(she)置(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)(he)樣(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)各(ge)(ge)加(jia)不(bu)(bu)同濃(nong)度(du)(du)(du)(du)的(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)50μL；3. 樣(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先加(jia)待(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)本(ben)(ben)(ben)10μL，再加(jia)樣(yang)(yang)本(ben)(ben)(ben)稀(xi)釋液(ye)(ye)(ye)(ye)(ye)40μL；空白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)。4. 除空白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)外(wai)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)(he)樣(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)入(ru)辣(la)根過(guo)(guo)氧化物(wu)(wu)酶(mei)(mei)(mei)（HRP）標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記的(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)抗(kang)(kang)體(ti)(ti)100μL，用封(feng)板(ban)(ban)膜(mo)封(feng)住(zhu)反(fan)應(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，37℃水(shui)浴鍋或恒溫(wen)(wen)(wen)箱溫(wen)(wen)(wen)育(yu)60min。5. 棄去(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，吸水(shui)紙上(shang)拍(pai)干(gan)(gan)，每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)滿(man)洗滌(di)液(ye)(ye)(ye)(ye)(ye)，靜置(zhi)1min，甩(shuai)去(qu)洗滌(di)液(ye)(ye)(ye)(ye)(ye)，吸水(shui)紙上(shang)拍(pai)干(gan)(gan)，如(ru)此重(zhong)復(fu)洗板(ban)(ban)5次(ci)(ci)（也可用洗板(ban)(ban)機洗板(ban)(ban)）。6. 每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)入(ru)底物(wu)(wu)A、B各(ge)(ge)50μL，37℃避(bi)光孵育(yu)15min。7. 每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)入(ru)終(zhong)止液(ye)(ye)(ye)(ye)(ye)50μL，15min內(nei)，在(zai)450nm波(bo)長處測(ce)(ce)(ce)(ce)定各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)OD值(zhi)。技(ji)術支持：電(dian)話：電(dian)子郵件：網址：fllygs.com生(sheng)(sheng)產廠家：上(shang)海(hai)篤(du)瑪生(sheng)(sheng)物(wu)(wu)科技(ji)有(you)(you)限公司(si)免責聲明：1. 試(shi)(shi)(shi)(shi)劑(ji)盒僅供研究使用，不(bu)(bu)得用于(yu)臨床實(shi)(shi)驗或人體(ti)(ti)實(shi)(shi)驗，否則所 產生(sheng)(sheng)的(de)(de)(de)一(yi)切后(hou)(hou)果，由實(shi)(shi)驗者(zhe)承擔，本(ben)(ben)(ben)公司(si)概(gai)不(bu)(bu)負責。2. 嚴格(ge)按照(zhao)(zhao)說(shuo)明書(shu)操(cao)作(zuo)，實(shi)(shi)驗者(zhe)違(wei)反(fan)說(shuo)明書(shu)操(cao)作(zuo)，后(hou)(hou)果由實(shi)(shi)驗者(zhe) 承擔
本(ben)(ben)試(shi)(shi)劑(ji)(ji)(ji)盒只能用(yong)(yong)(yong)于科學研究(jiu)，不(bu)得(de)用(yong)(yong)(yong)于醫(yi)學診斷 人（Human）N-乙酰轉(zhuan)(zhuan)(zhuan)移(yi)(yi)酶(mei)(mei) 10（NAT-10） ELISA 檢(jian)測(ce)(ce)試(shi)(shi)劑(ji)(ji)(ji)盒使(shi)(shi)用(yong)(yong)(yong)說(shuo)明書(shu)檢(jian)測(ce)(ce)原理(li)(li)試(shi)(shi)劑(ji)(ji)(ji)盒采(cai)用(yong)(yong)(yong)雙抗(kang)(kang)體(ti)一(yi)步(bu)(bu)夾心(xin)法(fa)(fa)酶(mei)(mei)聯免(mian)(mian)疫吸附(fu)試(shi)(shi)驗(yan)（ELISA）。往 預先包被N-乙酰轉(zhuan)(zhuan)(zhuan)移(yi)(yi)酶(mei)(mei)10（NAT-10）抗(kang)(kang)體(ti)的(de)(de)(de)(de)(de)包被微孔(kong)(kong)(kong)(kong)中(zhong)，依(yi)次(ci)加(jia)(jia)(jia)(jia)入(ru) 標(biao)(biao)本(ben)(ben)、標(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)、HRP標(biao)(biao)記(ji)的(de)(de)(de)(de)(de)檢(jian)測(ce)(ce)抗(kang)(kang)體(ti)，經過(guo)(guo)溫(wen)育(yu)并(bing)徹底(di)洗(xi)(xi)(xi)(xi)滌(di)(di)(di)。用(yong)(yong)(yong)底(di)物 TMB顯(xian)色(se)，TMB在(zai)過(guo)(guo)氧化(hua)物酶(mei)(mei)的(de)(de)(de)(de)(de)催化(hua)下轉(zhuan)(zhuan)(zhuan)化(hua)成(cheng)藍色(se)，并(bing)在(zai)酸的(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)下 轉(zhuan)(zhuan)(zhuan)化(hua)成(cheng)＊終的(de)(de)(de)(de)(de)黃色(se)。顏(yan)色(se)的(de)(de)(de)(de)(de)深淺和樣(yang)(yang)品(pin)(pin)中(zhong)的(de)(de)(de)(de)(de)N-乙酰轉(zhuan)(zhuan)(zhuan)移(yi)(yi)酶(mei)(mei)10（NAT-10）呈正相關。用(yong)(yong)(yong)酶(mei)(mei)標(biao)(biao)儀在(zai)450nm 波長(chang)下測(ce)(ce)定吸光(guang)度(du)(du)（OD 值）， 計算(suan)樣(yang)(yang)品(pin)(pin)活性。樣(yang)(yang)品(pin)(pin)收集、處理(li)(li)及保存(cun)方法(fa)(fa)1. 血(xue)清(qing)：使(shi)(shi)用(yong)(yong)(yong)不(bu)含熱(re)原和內(nei)毒素的(de)(de)(de)(de)(de)試(shi)(shi)管(guan)，操(cao)(cao)(cao)作(zuo)(zuo)過(guo)(guo)程中(zhong)避(bi)免(mian)(mian)任(ren)何細 胞(bao)刺(ci)激，收集血(xue)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)，3000 轉(zhuan)(zhuan)(zhuan)離心(xin) 10 分(fen)(fen)(fen)鐘將血(xue)清(qing)和紅細胞(bao)迅速(su)小心(xin) 地分(fen)(fen)(fen)離。 2. 血(xue)漿：EDTA、檸(ning)檬(meng)酸鹽或肝素抗(kang)(kang)凝。3000 轉(zhuan)(zhuan)(zhuan)離心(xin) 30 分(fen)(fen)(fen)鐘取上(shang)清(qing)。 3. 細胞(bao)上(shang)清(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)：3000 轉(zhuan)(zhuan)(zhuan)離心(xin) 10 分(fen)(fen)(fen)鐘去(qu)除顆粒和聚(ju)合物。 4. 組織勻漿：將組織加(jia)(jia)(jia)(jia)入(ru)適量生理(li)(li)鹽水(shui)搗碎。3000 轉(zhuan)(zhuan)(zhuan)離心(xin) 10 分(fen)(fen)(fen)鐘 取上(shang)清(qing)。 5. 保存(cun)：如果樣(yang)(yang)本(ben)(ben)收集后(hou)(hou)不(bu)及時(shi)檢(jian)測(ce)(ce)，請按(an)一(yi)次(ci)用(yong)(yong)(yong)量分(fen)(fen)(fen)裝，凍(dong)存(cun) 于-20℃，避(bi)免(mian)(mian)反(fan)復(fu)凍(dong)融，在(zai)室(shi)溫(wen)下解凍(dong)并(bing)確保樣(yang)(yang)品(pin)(pin)均勻地充分(fen)(fen)(fen)解凍(dong)。自備(bei)(bei)物品(pin)(pin)1. 酶(mei)(mei)標(biao)(biao)儀（450nm） 2. 高精度(du)(du)加(jia)(jia)(jia)(jia)樣(yang)(yang)器及槍(qiang)頭：0.5-10uL、2-20uL、20-200uL、200-1000uL 3. 37℃恒溫(wen)箱(xiang)(xiang)操(cao)(cao)(cao)作(zuo)(zuo)注(zhu)(zhu)意事項1. 試(shi)(shi)劑(ji)(ji)(ji)盒保存(cun)在(zai) 2-8℃，使(shi)(shi)用(yong)(yong)(yong)前室(shi)溫(wen)平衡 20 分(fen)(fen)(fen)鐘。從冰箱(xiang)(xiang)取出(chu)(chu)的(de)(de)(de)(de)(de) 濃(nong)縮洗(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)會有(you)結晶(jing)，這屬于正常現象(xiang)，水(shui)浴(yu)加(jia)(jia)(jia)(jia)熱(re)使(shi)(shi)結晶(jing)完全(quan)溶解 后(hou)(hou)再(zai)使(shi)(shi)用(yong)(yong)(yong)。 2. 實驗(yan)中(zhong)不(bu)用(yong)(yong)(yong)的(de)(de)(de)(de)(de)板(ban)條應(ying)立即(ji)放回(hui)自封(feng)袋中(zhong)，密封(feng)（低溫(wen)干燥）保 存(cun)。 3. 濃(nong)度(du)(du)為(wei) 0 的(de)(de)(de)(de)(de) S0 號標(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)即(ji)可(ke)視為(wei)陰(yin)性對(dui)照(zhao)或者空白(bai)；按(an)照(zhao)說(shuo)明 書(shu)操(cao)(cao)(cao)作(zuo)(zuo)時(shi)樣(yang)(yang)本(ben)(ben)已經稀釋(shi) 5 倍，＊終結果乘(cheng)以(yi) 5 才是樣(yang)(yang)本(ben)(ben)實際濃(nong)度(du)(du)。 4. 嚴格按(an)照(zhao)說(shuo)明書(shu)中(zhong)標(biao)(biao)明的(de)(de)(de)(de)(de)時(shi)間、加(jia)(jia)(jia)(jia)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)量及順序進行溫(wen)育(yu)操(cao)(cao)(cao)作(zuo)(zuo)。 5. 所有(you)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)組分(fen)(fen)(fen)使(shi)(shi)用(yong)(yong)(yong)前充分(fen)(fen)(fen)搖(yao)勻。試(shi)(shi)劑(ji)(ji)(ji)盒組成(cheng) 名稱 96 孔(kong)(kong)(kong)(kong)配(pei)置 48 孔(kong)(kong)(kong)(kong)配(pei)置 備(bei)(bei)注(zhu)(zhu)微孔(kong)(kong)(kong)(kong)酶(mei)(mei)標(biao)(biao)板(ban) 12 孔(kong)(kong)(kong)(kong)×8 條 12 孔(kong)(kong)(kong)(kong)×4 條 無(wu)(wu)(wu) 標(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin) 0.3mL*6 管(guan) 0.3mL*6 管(guan) 無(wu)(wu)(wu) 樣(yang)(yang)本(ben)(ben)稀釋(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 6mL 3mL 無(wu)(wu)(wu) 檢(jian)測(ce)(ce)抗(kang)(kang)體(ti)-HRP 10mL 5mL 無(wu)(wu)(wu) 20×洗(xi)(xi)(xi)(xi)滌(di)(di)(di)緩沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 25mL 15mL 按(an)說(shuo)明書(shu)進行稀釋(shi) 底(di)物 A 6mL 3mL 無(wu)(wu)(wu) 底(di)物 B 6mL 3mL 無(wu)(wu)(wu) 終止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 6mL 3mL 無(wu)(wu)(wu) 封(feng)板(ban)膜 2 張 2 張 無(wu)(wu)(wu) 說(shuo)明書(shu) 1 份(fen) 1 份(fen) 無(wu)(wu)(wu) 自封(feng)袋 1 個 1 個 無(wu)(wu)(wu) 注(zhu)(zhu)：標(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)（S0-S5）濃(nong)度(du)(du)依(yi)次(ci)為(wei)：0、25、50、100、200、400 U/L試(shi)(shi)劑(ji)(ji)(ji)的(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)備(bei)(bei)20×洗(xi)(xi)(xi)(xi)滌(di)(di)(di)緩沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)稀釋(shi)：蒸(zheng)餾水(shui)按(an) 1：20 稀釋(shi)，即(ji) 1 份(fen)的(de)(de)(de)(de)(de) 20×洗(xi)(xi)(xi)(xi)滌(di)(di)(di) 緩沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)加(jia)(jia)(jia)(jia) 19 份(fen)的(de)(de)(de)(de)(de)蒸(zheng)餾水(shui)。洗(xi)(xi)(xi)(xi)板(ban)方法(fa)(fa)1. 手工洗(xi)(xi)(xi)(xi)板(ban)：甩盡孔(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，每(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，靜(jing)置 1min 后(hou)(hou)甩盡 孔(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，在(zai)吸水(shui)紙上(shang)拍干，如此(ci)(ci)洗(xi)(xi)(xi)(xi)板(ban) 5 次(ci)。 2. 自動洗(xi)(xi)(xi)(xi)板(ban)機(ji)：每(mei)(mei)孔(kong)(kong)(kong)(kong)注(zhu)(zhu)入(ru)洗(xi)(xi)(xi)(xi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 350μL，浸泡 1min，洗(xi)(xi)(xi)(xi)板(ban) 5 次(ci)。操(cao)(cao)(cao)作(zuo)(zuo)步(bu)(bu)驟1. 從室(shi)溫(wen)平衡 20min 后(hou)(hou)的(de)(de)(de)(de)(de)鋁箔袋中(zhong)取出(chu)(chu)所需板(ban)條，剩余(yu)板(ban)條用(yong)(yong)(yong)自 封(feng)袋密封(feng)放回(hui) 4℃。 2. 設置標(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)孔(kong)(kong)(kong)(kong)和樣(yang)(yang)本(ben)(ben)孔(kong)(kong)(kong)(kong)，標(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)孔(kong)(kong)(kong)(kong)各(ge)加(jia)(jia)(jia)(jia)不(bu)同濃(nong)度(du)(du)的(de)(de)(de)(de)(de)標(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin) 50μ L； 3. 樣(yang)(yang)本(ben)(ben)孔(kong)(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)待測(ce)(ce)樣(yang)(yang)本(ben)(ben) 10μL，再(zai)加(jia)(jia)(jia)(jia)樣(yang)(yang)本(ben)(ben)稀釋(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 40μL；空白(bai)孔(kong)(kong)(kong)(kong)不(bu) 加(jia)(jia)(jia)(jia)。 4. 除空白(bai)孔(kong)(kong)(kong)(kong)外，標(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)孔(kong)(kong)(kong)(kong)和樣(yang)(yang)本(ben)(ben)孔(kong)(kong)(kong)(kong)中(zhong)每(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)辣根過(guo)(guo)氧化(hua)物酶(mei)(mei) （HRP）標(biao)(biao)記(ji)的(de)(de)(de)(de)(de)檢(jian)測(ce)(ce)抗(kang)(kang)體(ti) 100μL，用(yong)(yong)(yong)封(feng)板(ban)膜封(feng)住(zhu)反(fan)應(ying)孔(kong)(kong)(kong)(kong)，37℃水(shui)浴(yu)鍋 或恒溫(wen)箱(xiang)(xiang)溫(wen)育(yu) 60min。 5. 棄(qi)去(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，吸水(shui)紙上(shang)拍干，每(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，靜(jing)置 1min，甩去(qu) 洗(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，吸水(shui)紙上(shang)拍干，如此(ci)(ci)重復(fu)洗(xi)(xi)(xi)(xi)板(ban) 5 次(ci)（也可(ke)用(yong)(yong)(yong)洗(xi)(xi)(xi)(xi)板(ban)機(ji)洗(xi)(xi)(xi)(xi)板(ban)）。 6. 每(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)底(di)物 A、B 各(ge) 50μL，37℃避(bi)光(guang)孵育(yu) 15min。 7. 每(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)終止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 50μL，15min 內(nei)，在(zai) 450nm 波長(chang)處測(ce)(ce)定各(ge)孔(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de) OD 值。結果判斷繪制標(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲線(xian)(xian)(xian)：在(zai) Excel 工作(zuo)(zuo)表中(zhong)，以(yi)標(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)濃(nong)度(du)(du)作(zuo)(zuo)橫(heng)坐(zuo)標(biao)(biao)，對(dui)應(ying) OD 值作(zuo)(zuo)縱坐(zuo)標(biao)(biao)，繪制出(chu)(chu)標(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)線(xian)(xian)(xian)性回(hui)歸曲線(xian)(xian)(xian)，按(an)曲線(xian)(xian)(xian)方程計算(suan)各(ge)樣(yang)(yang) 本(ben)(ben)濃(nong)度(du)(du)值。
用于動力觀察,菌(jun)種(zhong)保存,H 抗原位(wei)相變異試驗成分(fen)(g/L) ：蛋(dan)白胨 10.0牛(niu)肉浸粉 ; 3.0瓊(qiong)脂 4.0氯化鈉 5.0pH值7.4 ± 0.1 25℃用法：稱取本品 22.0g ,加熱(re)溶解(jie)于 1000ml 蒸餾水中,煮沸溶解(jie),分(fen)裝(zhuang)小試管,121℃高壓滅菌(jun) 15 分(fen)鐘(zhong),直立凝固,備用。
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