小鼠（Mouse）腎(shen)上(shang)(shang)(shang)腺素(su)(su)(su)（EPI）ELISA檢(jian)測(ce)試(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)本(ben)(ben)試(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)只能(neng)用(yong)于(yu)(yu)(yu)科學(xue)研(yan)究(jiu)，不(bu)(bu)(bu)得用(yong)于(yu)(yu)(yu)醫學(xue)診斷使用(yong)說(shuo)明(ming)書(shu)(shu)(shu)產(chan)品(pin)(pin)(pin)貨號：【DM-X6731】產(chan)品(pin)(pin)(pin)規格：【48T/96T】檢(jian)測(ce)范(fan)圍：反應性(xing)：Mouse檢(jian)測(ce)范(fan)圍：0.25-16 ng/mL靈敏(min)度(du)(du)： 0.1 ng/mL檢(jian)測(ce)原理試(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)采用(yong)雙抗體(ti)(ti)(ti)(ti)一步夾心(xin)法(fa)酶聯免(mian)疫吸(xi)附試(shi)驗（ELISA）。往預先包被腎(shen)上(shang)(shang)(shang)腺素(su)(su)(su)（EPI）抗體(ti)(ti)(ti)(ti)的(de)(de)(de)包被微孔(kong)(kong)(kong)(kong)(kong)中(zhong)，依次(ci)(ci)加入標(biao)(biao)本(ben)(ben)、標(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)、HRP標(biao)(biao)記的(de)(de)(de)檢(jian)測(ce)抗體(ti)(ti)(ti)(ti)，經過(guo)(guo)溫(wen)(wen)育并徹(che)底(di)洗(xi)滌(di)(di)。用(yong)底(di)物(wu)TMB顯(xian)色，TMB在過(guo)(guo)氧化物(wu)酶的(de)(de)(de)催化下(xia)轉化成藍色，并在酸(suan)(suan)的(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)用(yong)下(xia)轉化成＊終(zhong)的(de)(de)(de)黃色。顏(yan)色的(de)(de)(de)深淺和(he)樣品(pin)(pin)(pin)中(zhong)的(de)(de)(de)腎(shen)上(shang)(shang)(shang)腺素(su)(su)(su)（EPI）呈正相關(guan)。用(yong)酶標(biao)(biao)儀(yi)在450nm 波長下(xia)測(ce)定吸(xi)光度(du)(du)（OD 值(zhi)），計(ji)算樣品(pin)(pin)(pin)濃(nong)(nong)度(du)(du)。樣品(pin)(pin)(pin)收(shou)集(ji)、處理及(ji)保存(cun)(cun)方法(fa)1. 血清：使用(yong)不(bu)(bu)(bu)含熱原和(he)內(nei)(nei)毒素(su)(su)(su)的(de)(de)(de)試(shi)管(guan)(guan)，操(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)過(guo)(guo)程(cheng)中(zhong)避(bi)免(mian)任(ren)何細(xi)胞(bao)(bao)(bao)刺激，收(shou)集(ji)血液(ye)后(hou)(hou)(hou)，3000轉離(li)心(xin)10分(fen)(fen)鐘將血清和(he)紅細(xi)胞(bao)(bao)(bao)迅速(su)小心(xin)地分(fen)(fen)離(li)。2. 血漿：EDTA、檸檬(meng)酸(suan)(suan)鹽(yan)或肝(gan)素(su)(su)(su)抗凝。3000轉離(li)心(xin)30分(fen)(fen)鐘取(qu)上(shang)(shang)(shang)清。3. 細(xi)胞(bao)(bao)(bao)上(shang)(shang)(shang)清液(ye)：3000轉離(li)心(xin)10分(fen)(fen)鐘去除(chu)顆粒和(he)聚合(he)物(wu)。4. 組織勻(yun)漿：將組織加入適量生(sheng)理鹽(yan)水(shui)(shui)搗碎。3000轉離(li)心(xin)10分(fen)(fen)鐘取(qu)上(shang)(shang)(shang)清。5. 保存(cun)(cun)：如果(guo)(guo)樣本(ben)(ben)收(shou)集(ji)后(hou)(hou)(hou)不(bu)(bu)(bu)及(ji)時(shi)(shi)檢(jian)測(ce)，請按一次(ci)(ci)用(yong)量分(fen)(fen)裝，凍(dong)存(cun)(cun)于(yu)(yu)(yu)-20℃，避(bi)免(mian)反復凍(dong)融，在室(shi)溫(wen)(wen)下(xia)解(jie)凍(dong)并確保樣品(pin)(pin)(pin)均勻(yun)地充分(fen)(fen)解(jie)凍(dong)。自備物(wu)品(pin)(pin)(pin)酶標(biao)(biao)儀(yi)（450nm）高精度(du)(du)加樣器(qi)及(ji)槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL37℃恒溫(wen)(wen)箱操(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)注意事項試(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)保存(cun)(cun)在2-8℃，使用(yong)前室(shi)溫(wen)(wen)平衡20分(fen)(fen)鐘。從(cong)冰(bing)箱取(qu)出(chu)的(de)(de)(de)濃(nong)(nong)縮洗(xi)滌(di)(di)液(ye)會有(you)結(jie)晶，這(zhe)屬于(yu)(yu)(yu)正常現象，水(shui)(shui)浴加熱使結(jie)晶完(wan)全溶解(jie)后(hou)(hou)(hou)再使用(yong)。實驗中(zhong)不(bu)(bu)(bu)用(yong)的(de)(de)(de)板(ban)(ban)(ban)條應立(li)即(ji)放回(hui)自封(feng)袋中(zhong)，密封(feng)（低溫(wen)(wen)干燥）保存(cun)(cun)。濃(nong)(nong)度(du)(du)為(wei)0的(de)(de)(de)S0號標(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)即(ji)可視為(wei)陰性(xing)對照或者空白；按照說(shuo)明(ming)書(shu)(shu)(shu)操(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)時(shi)(shi)樣本(ben)(ben)已(yi)經稀(xi)釋(shi)5倍(bei)，＊終(zhong)結(jie)果(guo)(guo)乘以5才(cai)是樣本(ben)(ben)實際濃(nong)(nong)度(du)(du)。嚴(yan)格按照說(shuo)明(ming)書(shu)(shu)(shu)中(zhong)標(biao)(biao)明(ming)的(de)(de)(de)時(shi)(shi)間(jian)、加液(ye)量及(ji)順序進(jin)行溫(wen)(wen)育操(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)。5. 所有(you)液(ye)體(ti)(ti)(ti)(ti)組分(fen)(fen)使用(yong)前充分(fen)(fen)搖勻(yun)。試(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)組成名稱(cheng)96孔(kong)(kong)(kong)(kong)(kong)配置48孔(kong)(kong)(kong)(kong)(kong)配置備注微孔(kong)(kong)(kong)(kong)(kong)酶標(biao)(biao)板(ban)(ban)(ban)12孔(kong)(kong)(kong)(kong)(kong)×8條12孔(kong)(kong)(kong)(kong)(kong)×4條無(wu)(wu)標(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)0.3mL*6管(guan)(guan)0.3mL*6管(guan)(guan)無(wu)(wu)樣本(ben)(ben)稀(xi)釋(shi)液(ye)6mL3mL無(wu)(wu)檢(jian)測(ce)抗體(ti)(ti)(ti)(ti)-HRP10mL5mL無(wu)(wu)20×洗(xi)滌(di)(di)緩沖液(ye)25mL15mL按說(shuo)明(ming)書(shu)(shu)(shu)進(jin)行稀(xi)釋(shi)底(di)物(wu)A6mL3mL無(wu)(wu)底(di)物(wu)B6mL3mL無(wu)(wu)終(zhong)止液(ye)6mL3mL無(wu)(wu)封(feng)板(ban)(ban)(ban)膜2張2張無(wu)(wu)說(shuo)明(ming)書(shu)(shu)(shu)1份(fen)1份(fen)無(wu)(wu)自封(feng)袋1個(ge)1個(ge)無(wu)(wu)注：標(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)（S0-S5）濃(nong)(nong)度(du)(du)依次(ci)(ci)為(wei)：0、1、2、4、8、16 ng/mL試(shi)劑(ji)(ji)(ji)(ji)的(de)(de)(de)準(zhun)(zhun)備 20×洗(xi)滌(di)(di)緩沖液(ye)的(de)(de)(de)稀(xi)釋(shi)：蒸(zheng)餾(liu)水(shui)(shui)按1：20稀(xi)釋(shi)，即(ji)1份(fen)的(de)(de)(de)20×洗(xi)滌(di)(di)緩沖液(ye)加19份(fen)的(de)(de)(de)蒸(zheng)餾(liu)水(shui)(shui)。洗(xi)板(ban)(ban)(ban)方法(fa)手工洗(xi)板(ban)(ban)(ban)：甩(shuai)盡孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)體(ti)(ti)(ti)(ti)，每(mei)孔(kong)(kong)(kong)(kong)(kong)加滿洗(xi)滌(di)(di)液(ye)，靜置1min后(hou)(hou)(hou)甩(shuai)盡孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)體(ti)(ti)(ti)(ti)，在吸(xi)水(shui)(shui)紙上(shang)(shang)(shang)拍干，如此洗(xi)板(ban)(ban)(ban)5次(ci)(ci)。自動洗(xi)板(ban)(ban)(ban)機：每(mei)孔(kong)(kong)(kong)(kong)(kong)注入洗(xi)液(ye)350μL，浸泡(pao)1min，洗(xi)板(ban)(ban)(ban)5次(ci)(ci)。操(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)步驟 結(jie)果(guo)(guo)判斷繪(hui)制(zhi)標(biao)(biao)準(zhun)(zhun)曲線(xian)(xian)：在Excel工作(zuo)(zuo)(zuo)(zuo)表中(zhong)，以標(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)濃(nong)(nong)度(du)(du)作(zuo)(zuo)(zuo)(zuo)橫坐(zuo)標(biao)(biao)，對應OD值(zhi)作(zuo)(zuo)(zuo)(zuo)縱坐(zuo)標(biao)(biao)，繪(hui)制(zhi)出(chu)標(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)線(xian)(xian)性(xing)回(hui)歸曲線(xian)(xian)，按曲線(xian)(xian)方程(cheng)計(ji)算各樣本(ben)(ben)濃(nong)(nong)度(du)(du)值(zhi)。試(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)性(xing)能(neng)準(zhun)(zhun)確性(xing)：標(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)線(xian)(xian)性(xing)回(hui)歸與預期(qi)濃(nong)(nong)度(du)(du)相關(guan)系(xi)數R值(zhi)，大(da)于(yu)(yu)(yu)等于(yu)(yu)(yu)0.9900。靈敏(min)度(du)(du)：＊低檢(jian)測(ce)濃(nong)(nong)度(du)(du)小于(yu)(yu)(yu)1.0 pg/mL。特異性(xing)：不(bu)(bu)(bu)與其它可溶性(xing)結(jie)構類似物(wu)交叉反應。重復性(xing)：板(ban)(ban)(ban)內(nei)(nei)、板(ban)(ban)(ban)間(jian)變異系(xi)數均小于(yu)(yu)(yu)15%。貯藏：2-8℃，避(bi)光防潮保存(cun)(cun)。有(you)效期(qi)：6個(ge)月(yue)免(mian)責(ze)聲明(ming)試(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)僅供(gong)研(yan)究(jiu)使用(yong)，不(bu)(bu)(bu)得用(yong)于(yu)(yu)(yu)臨床實驗或人體(ti)(ti)(ti)(ti)實驗，否則(ze)所產(chan)生(sheng)的(de)(de)(de)一切后(hou)(hou)(hou)果(guo)(guo)，由實驗者承擔(dan)(dan)，本(ben)(ben)公(gong)司(si)(si)概不(bu)(bu)(bu)負責(ze)。嚴(yan)格按照說(shuo)明(ming)書(shu)(shu)(shu)操(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)，實驗者違反說(shuo)明(ming)書(shu)(shu)(shu)操(cao)(cao)(cao)(cao)作(zuo)(zuo)(zuo)(zuo)，后(hou)(hou)(hou)果(guo)(guo)由實驗者承擔(dan)(dan)。技術支(zhi)持(chi)電話：電子郵件：網(wang)址：fllygs.com生(sheng)產(chan)廠家：上(shang)(shang)(shang)海篤(du)瑪生(sheng)物(wu)科技有(you)限(xian)公(gong)司(si)(si)
小(xiao)(xiao)鼠（Mouse）5羥(qian)(qian)(qian)色(se)(se)胺（5-HT）ELISA檢(jian)測(ce)(ce)試(shi)(shi)劑(ji)(ji)盒本試(shi)(shi)劑(ji)(ji)盒只能用(yong)(yong)于(yu)(yu)(yu)科學研究，不(bu)得(de)用(yong)(yong)于(yu)(yu)(yu)醫學診斷(duan)使用(yong)(yong)說(shuo)(shuo)明(ming)(ming)(ming)書(shu)產(chan)(chan)(chan)品(pin)貨(huo)號(hao)：【DM-X6730】產(chan)(chan)(chan)品(pin)規格：【48T/96T】檢(jian)測(ce)(ce)范圍：反(fan)應(ying)(ying)性(xing)(xing)：Mouse檢(jian)測(ce)(ce)范圍：1.75-240 ng/mL靈(ling)敏度(du)： 1.0 ng/mL檢(jian)測(ce)(ce)原理試(shi)(shi)劑(ji)(ji)盒采用(yong)(yong)雙抗體(ti)(ti)(ti)(ti)一(yi)步夾心法酶(mei)聯免疫吸附試(shi)(shi)驗(yan)（ELISA）。往(wang)預先包被5羥(qian)(qian)(qian)色(se)(se)胺（5-HT）抗體(ti)(ti)(ti)(ti)的(de)(de)(de)包被微孔(kong)中(zhong)，依(yi)(yi)次(ci)加(jia)入標(biao)(biao)(biao)(biao)本、標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)、HRP標(biao)(biao)(biao)(biao)記的(de)(de)(de)檢(jian)測(ce)(ce)抗體(ti)(ti)(ti)(ti)，經過溫(wen)(wen)育并(bing)(bing)徹底(di)洗(xi)(xi)(xi)滌(di)(di)(di)。用(yong)(yong)底(di)物(wu)TMB顯色(se)(se)，TMB在(zai)過氧化物(wu)酶(mei)的(de)(de)(de)催(cui)化下(xia)(xia)(xia)轉化成(cheng)(cheng)藍色(se)(se)，并(bing)(bing)在(zai)酸(suan)(suan)的(de)(de)(de)作(zuo)(zuo)用(yong)(yong)下(xia)(xia)(xia)轉化成(cheng)(cheng)＊終的(de)(de)(de)黃色(se)(se)。顏色(se)(se)的(de)(de)(de)深淺和樣(yang)品(pin)中(zhong)的(de)(de)(de)5羥(qian)(qian)(qian)色(se)(se)胺（5-HT）呈(cheng)正相關。用(yong)(yong)酶(mei)標(biao)(biao)(biao)(biao)儀在(zai)450nm 波長下(xia)(xia)(xia)測(ce)(ce)定吸光(guang)度(du)（OD 值），計算樣(yang)品(pin)濃(nong)(nong)度(du)。樣(yang)品(pin)收集、處理及(ji)保存(cun)(cun)方(fang)(fang)法1. 血清(qing)：使用(yong)(yong)不(bu)含熱原和內毒(du)素(su)(su)的(de)(de)(de)試(shi)(shi)管(guan)，操(cao)作(zuo)(zuo)過程中(zhong)避(bi)免任何細胞(bao)刺激，收集血液(ye)(ye)(ye)(ye)(ye)后，3000轉離(li)心10分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)將(jiang)血清(qing)和紅細胞(bao)迅速小(xiao)(xiao)心地分(fen)(fen)(fen)(fen)離(li)。2. 血漿：EDTA、檸檬酸(suan)(suan)鹽或(huo)(huo)(huo)肝素(su)(su)抗凝(ning)。3000轉離(li)心30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)取上(shang)清(qing)。3. 細胞(bao)上(shang)清(qing)液(ye)(ye)(ye)(ye)(ye)：3000轉離(li)心10分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)去除顆粒和聚合物(wu)。4. 組(zu)(zu)織(zhi)勻(yun)漿：將(jiang)組(zu)(zu)織(zhi)加(jia)入適量生理鹽水(shui)(shui)搗碎。3000轉離(li)心10分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)取上(shang)清(qing)。5. 保存(cun)(cun)：如果樣(yang)本收集后不(bu)及(ji)時檢(jian)測(ce)(ce)，請按一(yi)次(ci)用(yong)(yong)量分(fen)(fen)(fen)(fen)裝(zhuang)，凍存(cun)(cun)于(yu)(yu)(yu)-20℃，避(bi)免反(fan)復凍融(rong)，在(zai)室溫(wen)(wen)下(xia)(xia)(xia)解(jie)凍并(bing)(bing)確(que)保樣(yang)品(pin)均勻(yun)地充分(fen)(fen)(fen)(fen)解(jie)凍。自備物(wu)品(pin)酶(mei)標(biao)(biao)(biao)(biao)儀（450nm）高精度(du)加(jia)樣(yang)器及(ji)槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL37℃恒(heng)溫(wen)(wen)箱(xiang)操(cao)作(zuo)(zuo)注(zhu)(zhu)(zhu)(zhu)意事項試(shi)(shi)劑(ji)(ji)盒保存(cun)(cun)在(zai)2-8℃，使用(yong)(yong)前(qian)室溫(wen)(wen)平衡(heng)20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。從冰箱(xiang)取出的(de)(de)(de)濃(nong)(nong)縮(suo)洗(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)會有(you)結(jie)晶(jing)，這屬于(yu)(yu)(yu)正常現象，水(shui)(shui)浴加(jia)熱使結(jie)晶(jing)完全(quan)溶解(jie)后再使用(yong)(yong)。實(shi)驗(yan)中(zhong)不(bu)用(yong)(yong)的(de)(de)(de)板(ban)(ban)條應(ying)(ying)立即(ji)放回(hui)自封(feng)(feng)袋中(zhong)，密封(feng)(feng)（低溫(wen)(wen)干燥）保存(cun)(cun)。濃(nong)(nong)度(du)為(wei)0的(de)(de)(de)S0號(hao)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)即(ji)可視為(wei)陰性(xing)(xing)對(dui)照或(huo)(huo)(huo)者空白；按照說(shuo)(shuo)明(ming)(ming)(ming)書(shu)操(cao)作(zuo)(zuo)時樣(yang)本已經稀(xi)釋(shi)5倍，＊終結(jie)果乘以5才(cai)是(shi)樣(yang)本實(shi)際濃(nong)(nong)度(du)。嚴格按照說(shuo)(shuo)明(ming)(ming)(ming)書(shu)中(zhong)標(biao)(biao)(biao)(biao)明(ming)(ming)(ming)的(de)(de)(de)時間、加(jia)液(ye)(ye)(ye)(ye)(ye)量及(ji)順(shun)序進行溫(wen)(wen)育操(cao)作(zuo)(zuo)。5. 所有(you)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)組(zu)(zu)分(fen)(fen)(fen)(fen)使用(yong)(yong)前(qian)充分(fen)(fen)(fen)(fen)搖(yao)勻(yun)。試(shi)(shi)劑(ji)(ji)盒組(zu)(zu)成(cheng)(cheng)名稱(cheng)96孔(kong)配(pei)置48孔(kong)配(pei)置備注(zhu)(zhu)(zhu)(zhu)微孔(kong)酶(mei)標(biao)(biao)(biao)(biao)板(ban)(ban)12孔(kong)×8條12孔(kong)×4條無標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)0.3mL*6管(guan)0.3mL*6管(guan)無樣(yang)本稀(xi)釋(shi)液(ye)(ye)(ye)(ye)(ye)6mL3mL無檢(jian)測(ce)(ce)抗體(ti)(ti)(ti)(ti)-HRP10mL5mL無20×洗(xi)(xi)(xi)滌(di)(di)(di)緩沖液(ye)(ye)(ye)(ye)(ye)25mL15mL按說(shuo)(shuo)明(ming)(ming)(ming)書(shu)進行稀(xi)釋(shi)底(di)物(wu)A6mL3mL無底(di)物(wu)B6mL3mL無終止液(ye)(ye)(ye)(ye)(ye)6mL3mL無封(feng)(feng)板(ban)(ban)膜(mo)2張2張無說(shuo)(shuo)明(ming)(ming)(ming)書(shu)1份1份無自封(feng)(feng)袋1個(ge)1個(ge)無注(zhu)(zhu)(zhu)(zhu)：標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)（S0-S5）濃(nong)(nong)度(du)依(yi)(yi)次(ci)為(wei)：0、15、30、60、120、240 ng/mL試(shi)(shi)劑(ji)(ji)的(de)(de)(de)準(zhun)(zhun)備 20×洗(xi)(xi)(xi)滌(di)(di)(di)緩沖液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)稀(xi)釋(shi)：蒸餾(liu)水(shui)(shui)按1：20稀(xi)釋(shi)，即(ji)1份的(de)(de)(de)20×洗(xi)(xi)(xi)滌(di)(di)(di)緩沖液(ye)(ye)(ye)(ye)(ye)加(jia)19份的(de)(de)(de)蒸餾(liu)水(shui)(shui)。洗(xi)(xi)(xi)板(ban)(ban)方(fang)(fang)法手(shou)工洗(xi)(xi)(xi)板(ban)(ban)：甩(shuai)(shuai)盡(jin)孔(kong)內液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，每孔(kong)加(jia)滿(man)洗(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)，靜置1min后甩(shuai)(shuai)盡(jin)孔(kong)內液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，在(zai)吸水(shui)(shui)紙上(shang)拍干，如此洗(xi)(xi)(xi)板(ban)(ban)5次(ci)。自動(dong)洗(xi)(xi)(xi)板(ban)(ban)機：每孔(kong)注(zhu)(zhu)(zhu)(zhu)入洗(xi)(xi)(xi)液(ye)(ye)(ye)(ye)(ye)350μL，浸泡1min，洗(xi)(xi)(xi)板(ban)(ban)5次(ci)。操(cao)作(zuo)(zuo)步驟 結(jie)果判(pan)斷(duan)繪(hui)制標(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)線(xian)：在(zai)Excel工作(zuo)(zuo)表中(zhong)，以標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)濃(nong)(nong)度(du)作(zuo)(zuo)橫坐標(biao)(biao)(biao)(biao)，對(dui)應(ying)(ying)OD值作(zuo)(zuo)縱坐標(biao)(biao)(biao)(biao)，繪(hui)制出標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)線(xian)性(xing)(xing)回(hui)歸曲(qu)線(xian)，按曲(qu)線(xian)方(fang)(fang)程計算各(ge)樣(yang)本濃(nong)(nong)度(du)值。試(shi)(shi)劑(ji)(ji)盒性(xing)(xing)能準(zhun)(zhun)確(que)性(xing)(xing)：標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)線(xian)性(xing)(xing)回(hui)歸與(yu)預期(qi)濃(nong)(nong)度(du)相關系數R值，大于(yu)(yu)(yu)等于(yu)(yu)(yu)0.9900。靈(ling)敏度(du)：＊低檢(jian)測(ce)(ce)濃(nong)(nong)度(du)小(xiao)(xiao)于(yu)(yu)(yu)1.0 pg/mL。特(te)異性(xing)(xing)：不(bu)與(yu)其它可溶性(xing)(xing)結(jie)構類似物(wu)交(jiao)叉反(fan)應(ying)(ying)。重復性(xing)(xing)：板(ban)(ban)內、板(ban)(ban)間變異系數均小(xiao)(xiao)于(yu)(yu)(yu)15%。貯藏(zang)：2-8℃，避(bi)光(guang)防潮保存(cun)(cun)。有(you)效(xiao)期(qi)：6個(ge)月免責(ze)聲明(ming)(ming)(ming)試(shi)(shi)劑(ji)(ji)盒僅供研究使用(yong)(yong)，不(bu)得(de)用(yong)(yong)于(yu)(yu)(yu)臨(lin)床實(shi)驗(yan)或(huo)(huo)(huo)人體(ti)(ti)(ti)(ti)實(shi)驗(yan)，否(fou)則(ze)所產(chan)(chan)(chan)生的(de)(de)(de)一(yi)切后果，由實(shi)驗(yan)者承擔(dan)，本公司(si)概不(bu)負(fu)責(ze)。嚴格按照說(shuo)(shuo)明(ming)(ming)(ming)書(shu)操(cao)作(zuo)(zuo)，實(shi)驗(yan)者違反(fan)說(shuo)(shuo)明(ming)(ming)(ming)書(shu)操(cao)作(zuo)(zuo)，后果由實(shi)驗(yan)者承擔(dan)。技(ji)術(shu)支持電(dian)話：電(dian)子郵(you)件：網址(zhi)：fllygs.com生產(chan)(chan)(chan)廠家：上(shang)海篤(du)瑪(ma)生物(wu)科技(ji)有(you)限公司(si)
大(da)鼠（Rat）色氨酸(suan)(suan)（TRP）ELISA檢(jian)(jian)(jian)測(ce)試(shi)劑(ji)盒(he)本(ben)試(shi)劑(ji)盒(he)只能(neng)用(yong)(yong)于(yu)(yu)科(ke)學(xue)研(yan)究，不(bu)得用(yong)(yong)于(yu)(yu)醫學(xue)診斷使(shi)(shi)(shi)(shi)用(yong)(yong)說(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)貨(huo)號(hao)：【DM-D9691】產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)規(gui)格：【48T/96T】檢(jian)(jian)(jian)測(ce)范(fan)圍(wei)(wei)：反應(ying)性(xing)：Rat檢(jian)(jian)(jian)測(ce)范(fan)圍(wei)(wei)：3.66-480 pg/mL靈(ling)敏(min)度(du)(du)(du)： ＊低(di)檢(jian)(jian)(jian)測(ce)濃(nong)(nong)(nong)(nong)度(du)(du)(du)小(xiao)(xiao)于(yu)(yu)1.0 pg/mL檢(jian)(jian)(jian)測(ce)原理試(shi)劑(ji)盒(he)采用(yong)(yong)雙抗體(ti)一(yi)(yi)步夾(jia)心(xin)法酶(mei)聯免疫吸附試(shi)驗(yan)(yan)（ELISA）。往預先包被色氨酸(suan)(suan)（TRP）抗體(ti)的(de)(de)包被微孔(kong)(kong)中(zhong)(zhong)(zhong)，依次(ci)加(jia)(jia)(jia)入標(biao)(biao)(biao)本(ben)、標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)記的(de)(de)檢(jian)(jian)(jian)測(ce)抗體(ti)，經(jing)過溫育并(bing)徹底洗(xi)(xi)(xi)(xi)滌(di)。用(yong)(yong)底物TMB顯色，TMB在(zai)(zai)(zai)過氧化(hua)物酶(mei)的(de)(de)催(cui)化(hua)下轉(zhuan)化(hua)成藍色，并(bing)在(zai)(zai)(zai)酸(suan)(suan)的(de)(de)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)下轉(zhuan)化(hua)成＊終的(de)(de)黃(huang)色。顏色的(de)(de)深淺和樣(yang)(yang)品(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)的(de)(de)色氨酸(suan)(suan)（TRP）呈(cheng)正相(xiang)關。用(yong)(yong)酶(mei)標(biao)(biao)(biao)儀在(zai)(zai)(zai)450nm 波長下測(ce)定吸光度(du)(du)(du)（OD 值(zhi)），計算樣(yang)(yang)品(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)。樣(yang)(yang)品(pin)(pin)(pin)(pin)收(shou)集(ji)、處理及保(bao)存(cun)方(fang)(fang)法1. 血(xue)清(qing)：使(shi)(shi)(shi)(shi)用(yong)(yong)不(bu)含熱(re)原和內(nei)毒(du)素的(de)(de)試(shi)管(guan)(guan)，操(cao)作(zuo)(zuo)(zuo)(zuo)過程中(zhong)(zhong)(zhong)避(bi)(bi)免任(ren)何細胞刺(ci)激，收(shou)集(ji)血(xue)液后(hou)，3000轉(zhuan)離(li)心(xin)10分鐘將血(xue)清(qing)和紅(hong)細胞迅速(su)小(xiao)(xiao)心(xin)地(di)分離(li)。2. 血(xue)漿(jiang)：EDTA、檸檬酸(suan)(suan)鹽或(huo)肝素抗凝。3000轉(zhuan)離(li)心(xin)30分鐘取(qu)上清(qing)。3. 細胞上清(qing)液：3000轉(zhuan)離(li)心(xin)10分鐘去除顆粒(li)和聚合物。4. 組織勻漿(jiang)：將組織加(jia)(jia)(jia)入適量生(sheng)(sheng)理鹽水搗碎(sui)。3000轉(zhuan)離(li)心(xin)10分鐘取(qu)上清(qing)。5. 保(bao)存(cun)：如果樣(yang)(yang)本(ben)收(shou)集(ji)后(hou)不(bu)及時(shi)檢(jian)(jian)(jian)測(ce)，請按(an)一(yi)(yi)次(ci)用(yong)(yong)量分裝，凍(dong)存(cun)于(yu)(yu)-20℃，避(bi)(bi)免反復(fu)凍(dong)融，在(zai)(zai)(zai)室(shi)溫下解(jie)凍(dong)并(bing)確保(bao)樣(yang)(yang)品(pin)(pin)(pin)(pin)均(jun)勻地(di)充分解(jie)凍(dong)。自備(bei)(bei)物品(pin)(pin)(pin)(pin)酶(mei)標(biao)(biao)(biao)儀（450nm）高精度(du)(du)(du)加(jia)(jia)(jia)樣(yang)(yang)器及槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL37℃恒溫箱操(cao)作(zuo)(zuo)(zuo)(zuo)注意事項試(shi)劑(ji)盒(he)保(bao)存(cun)在(zai)(zai)(zai)2-8℃，使(shi)(shi)(shi)(shi)用(yong)(yong)前室(shi)溫平衡(heng)20分鐘。從冰箱取(qu)出的(de)(de)濃(nong)(nong)(nong)(nong)縮洗(xi)(xi)(xi)(xi)滌(di)液會有結(jie)晶，這屬(shu)于(yu)(yu)正常現象，水浴(yu)加(jia)(jia)(jia)熱(re)使(shi)(shi)(shi)(shi)結(jie)晶完全溶(rong)(rong)解(jie)后(hou)再使(shi)(shi)(shi)(shi)用(yong)(yong)。實(shi)驗(yan)(yan)中(zhong)(zhong)(zhong)不(bu)用(yong)(yong)的(de)(de)板(ban)(ban)(ban)條應(ying)立即(ji)放(fang)回自封(feng)袋中(zhong)(zhong)(zhong)，密封(feng)（低(di)溫干燥）保(bao)存(cun)。濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)0的(de)(de)S0號(hao)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)即(ji)可視為(wei)陰性(xing)對照或(huo)者空白；按(an)照說(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)操(cao)作(zuo)(zuo)(zuo)(zuo)時(shi)樣(yang)(yang)本(ben)已經(jing)稀釋(shi)5倍(bei)，＊終結(jie)果乘(cheng)以(yi)5才是樣(yang)(yang)本(ben)實(shi)際濃(nong)(nong)(nong)(nong)度(du)(du)(du)。嚴(yan)(yan)格按(an)照說(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)中(zhong)(zhong)(zhong)標(biao)(biao)(biao)明(ming)(ming)(ming)的(de)(de)時(shi)間(jian)(jian)、加(jia)(jia)(jia)液量及順序進行溫育操(cao)作(zuo)(zuo)(zuo)(zuo)。5. 所有液體(ti)組分使(shi)(shi)(shi)(shi)用(yong)(yong)前充分搖(yao)勻。試(shi)劑(ji)盒(he)組成名稱96孔(kong)(kong)配置(zhi)48孔(kong)(kong)配置(zhi)備(bei)(bei)注微孔(kong)(kong)酶(mei)標(biao)(biao)(biao)板(ban)(ban)(ban)12孔(kong)(kong)×8條12孔(kong)(kong)×4條無標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)0.3mL*6管(guan)(guan)0.3mL*6管(guan)(guan)無樣(yang)(yang)本(ben)稀釋(shi)液6mL3mL無檢(jian)(jian)(jian)測(ce)抗體(ti)-HRP10mL5mL無20×洗(xi)(xi)(xi)(xi)滌(di)緩(huan)沖(chong)(chong)液25mL15mL按(an)說(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)進行稀釋(shi)底物A6mL3mL無底物B6mL3mL無終止液6mL3mL無封(feng)板(ban)(ban)(ban)膜2張(zhang)2張(zhang)無說(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)1份1份無自封(feng)袋1個1個無注：標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)（S0-S5）濃(nong)(nong)(nong)(nong)度(du)(du)(du)依次(ci)為(wei)：0、30、60、120、240、480 pg/mL試(shi)劑(ji)的(de)(de)準(zhun)(zhun)(zhun)(zhun)備(bei)(bei) 20×洗(xi)(xi)(xi)(xi)滌(di)緩(huan)沖(chong)(chong)液的(de)(de)稀釋(shi)：蒸(zheng)餾水按(an)1：20稀釋(shi)，即(ji)1份的(de)(de)20×洗(xi)(xi)(xi)(xi)滌(di)緩(huan)沖(chong)(chong)液加(jia)(jia)(jia)19份的(de)(de)蒸(zheng)餾水。洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)方(fang)(fang)法手工(gong)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)：甩盡孔(kong)(kong)內(nei)液體(ti)，每孔(kong)(kong)加(jia)(jia)(jia)滿洗(xi)(xi)(xi)(xi)滌(di)液，靜(jing)置(zhi)1min后(hou)甩盡孔(kong)(kong)內(nei)液體(ti)，在(zai)(zai)(zai)吸水紙(zhi)上拍干，如此洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)5次(ci)。自動(dong)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)機：每孔(kong)(kong)注入洗(xi)(xi)(xi)(xi)液350μL，浸泡1min，洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)5次(ci)。操(cao)作(zuo)(zuo)(zuo)(zuo)步驟(zou) 結(jie)果判(pan)斷繪(hui)制(zhi)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)：在(zai)(zai)(zai)Excel工(gong)作(zuo)(zuo)(zuo)(zuo)表中(zhong)(zhong)(zhong)，以(yi)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)作(zuo)(zuo)(zuo)(zuo)橫(heng)坐標(biao)(biao)(biao)，對應(ying)OD值(zhi)作(zuo)(zuo)(zuo)(zuo)縱坐標(biao)(biao)(biao)，繪(hui)制(zhi)出標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)線(xian)性(xing)回歸曲(qu)(qu)線(xian)，按(an)曲(qu)(qu)線(xian)方(fang)(fang)程計算各樣(yang)(yang)本(ben)濃(nong)(nong)(nong)(nong)度(du)(du)(du)值(zhi)。試(shi)劑(ji)盒(he)性(xing)能(neng)準(zhun)(zhun)(zhun)(zhun)確性(xing)：標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)線(xian)性(xing)回歸與(yu)預期濃(nong)(nong)(nong)(nong)度(du)(du)(du)相(xiang)關系數R值(zhi)，大(da)于(yu)(yu)等于(yu)(yu)0.9900。靈(ling)敏(min)度(du)(du)(du)：＊低(di)檢(jian)(jian)(jian)測(ce)濃(nong)(nong)(nong)(nong)度(du)(du)(du)小(xiao)(xiao)于(yu)(yu)1.0 pg/mL。特異(yi)性(xing)：不(bu)與(yu)其(qi)它可溶(rong)(rong)性(xing)結(jie)構(gou)類(lei)似物交叉反應(ying)。重復(fu)性(xing)：板(ban)(ban)(ban)內(nei)、板(ban)(ban)(ban)間(jian)(jian)變異(yi)系數均(jun)小(xiao)(xiao)于(yu)(yu)15%。貯藏：2-8℃，避(bi)(bi)光防潮(chao)保(bao)存(cun)。有效(xiao)期：6個月免責(ze)聲(sheng)明(ming)(ming)(ming)試(shi)劑(ji)盒(he)僅供研(yan)究使(shi)(shi)(shi)(shi)用(yong)(yong)，不(bu)得用(yong)(yong)于(yu)(yu)臨床實(shi)驗(yan)(yan)或(huo)人體(ti)實(shi)驗(yan)(yan)，否則所產(chan)(chan)(chan)生(sheng)(sheng)的(de)(de)一(yi)(yi)切后(hou)果，由(you)實(shi)驗(yan)(yan)者承擔(dan)(dan)，本(ben)公司概不(bu)負責(ze)。嚴(yan)(yan)格按(an)照說(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)操(cao)作(zuo)(zuo)(zuo)(zuo)，實(shi)驗(yan)(yan)者違(wei)反說(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)操(cao)作(zuo)(zuo)(zuo)(zuo)，后(hou)果由(you)實(shi)驗(yan)(yan)者承擔(dan)(dan)。技術支持電(dian)話：電(dian)子(zi)郵件：網(wang)址(zhi)：fllygs.com生(sheng)(sheng)產(chan)(chan)(chan)廠家：上海篤瑪生(sheng)(sheng)物科(ke)技有限公司
大(da)鼠（Rat）尿氨(an)(an)酸（Kynurenine）ELISA檢(jian)測(ce)(ce)(ce)試(shi)(shi)(shi)劑(ji)(ji)盒本(ben)試(shi)(shi)(shi)劑(ji)(ji)盒只能用(yong)(yong)(yong)(yong)于(yu)科學研(yan)究(jiu)，不(bu)(bu)得用(yong)(yong)(yong)(yong)于(yu)醫學診斷使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)說(shuo)明(ming)(ming)書(shu)(shu)產品(pin)(pin)(pin)貨號：【DM-D9690】產品(pin)(pin)(pin)規(gui)格(ge)(ge)：【48T/96T】檢(jian)測(ce)(ce)(ce)范圍：反(fan)應(ying)性(xing)：Rat檢(jian)測(ce)(ce)(ce)范圍：7.13-480 pg/mL靈敏(min)度(du)(du)： ＊低檢(jian)測(ce)(ce)(ce)濃(nong)度(du)(du)小于(yu)1.0 pg/mL檢(jian)測(ce)(ce)(ce)原理(li)試(shi)(shi)(shi)劑(ji)(ji)盒采用(yong)(yong)(yong)(yong)雙(shuang)抗體一(yi)步夾心法酶(mei)(mei)(mei)聯免疫吸(xi)附試(shi)(shi)(shi)驗（ELISA）。往預(yu)先(xian)包(bao)被(bei)大(da)鼠尿氨(an)(an)酸（Kynurenine）抗體的(de)(de)(de)(de)包(bao)被(bei)微(wei)孔(kong)中(zhong)(zhong)，依次(ci)加(jia)(jia)入(ru)標(biao)(biao)(biao)(biao)本(ben)、標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)檢(jian)測(ce)(ce)(ce)抗體，經過溫育(yu)并徹底洗(xi)滌(di)。用(yong)(yong)(yong)(yong)底物(wu)(wu)(wu)TMB顯色，TMB在(zai)過氧化物(wu)(wu)(wu)酶(mei)(mei)(mei)的(de)(de)(de)(de)催化下轉(zhuan)(zhuan)化成(cheng)藍(lan)色，并在(zai)酸的(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)下轉(zhuan)(zhuan)化成(cheng)＊終的(de)(de)(de)(de)黃色。顏色的(de)(de)(de)(de)深淺和(he)樣(yang)(yang)品(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)犬(quan)尿氨(an)(an)酸（Kynurenine）呈正相關。用(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)儀(yi)在(zai)450nm 波長下測(ce)(ce)(ce)定(ding)吸(xi)光度(du)(du)（OD 值），計算樣(yang)(yang)品(pin)(pin)(pin)濃(nong)度(du)(du)。樣(yang)(yang)品(pin)(pin)(pin)收集、處理(li)及(ji)保存方(fang)(fang)法1. 血(xue)(xue)清(qing)：使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)不(bu)(bu)含熱(re)原和(he)內毒素(su)的(de)(de)(de)(de)試(shi)(shi)(shi)管(guan)，操(cao)(cao)作(zuo)(zuo)(zuo)(zuo)過程中(zhong)(zhong)避(bi)免任何細(xi)胞(bao)(bao)刺激，收集血(xue)(xue)液(ye)(ye)(ye)(ye)后，3000轉(zhuan)(zhuan)離心10分(fen)(fen)鐘(zhong)將(jiang)血(xue)(xue)清(qing)和(he)紅細(xi)胞(bao)(bao)迅速(su)小心地(di)分(fen)(fen)離。2. 血(xue)(xue)漿：EDTA、檸檬酸鹽(yan)或肝素(su)抗凝。3000轉(zhuan)(zhuan)離心30分(fen)(fen)鐘(zhong)取(qu)上(shang)(shang)(shang)清(qing)。3. 細(xi)胞(bao)(bao)上(shang)(shang)(shang)清(qing)液(ye)(ye)(ye)(ye)：3000轉(zhuan)(zhuan)離心10分(fen)(fen)鐘(zhong)去除顆粒和(he)聚合物(wu)(wu)(wu)。4. 組織勻(yun)漿：將(jiang)組織加(jia)(jia)入(ru)適量生(sheng)理(li)鹽(yan)水(shui)搗碎。3000轉(zhuan)(zhuan)離心10分(fen)(fen)鐘(zhong)取(qu)上(shang)(shang)(shang)清(qing)。5. 保存：如果(guo)(guo)樣(yang)(yang)本(ben)收集后不(bu)(bu)及(ji)時(shi)檢(jian)測(ce)(ce)(ce)，請按(an)一(yi)次(ci)用(yong)(yong)(yong)(yong)量分(fen)(fen)裝(zhuang)，凍(dong)(dong)(dong)存于(yu)-20℃，避(bi)免反(fan)復(fu)凍(dong)(dong)(dong)融，在(zai)室(shi)溫下解(jie)凍(dong)(dong)(dong)并確(que)保樣(yang)(yang)品(pin)(pin)(pin)均勻(yun)地(di)充分(fen)(fen)解(jie)凍(dong)(dong)(dong)。自(zi)(zi)備(bei)(bei)(bei)物(wu)(wu)(wu)品(pin)(pin)(pin)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)儀(yi)（450nm）高精(jing)度(du)(du)加(jia)(jia)樣(yang)(yang)器及(ji)槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL37℃恒溫箱操(cao)(cao)作(zuo)(zuo)(zuo)(zuo)注(zhu)(zhu)意事項試(shi)(shi)(shi)劑(ji)(ji)盒保存在(zai)2-8℃，使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前(qian)室(shi)溫平衡20分(fen)(fen)鐘(zhong)。從冰箱取(qu)出的(de)(de)(de)(de)濃(nong)縮洗(xi)滌(di)液(ye)(ye)(ye)(ye)會(hui)有結(jie)晶(jing)(jing)，這屬于(yu)正常現象(xiang)，水(shui)浴(yu)加(jia)(jia)熱(re)使(shi)(shi)(shi)結(jie)晶(jing)(jing)完全溶解(jie)后再使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)。實(shi)驗中(zhong)(zhong)不(bu)(bu)用(yong)(yong)(yong)(yong)的(de)(de)(de)(de)板(ban)條應(ying)立(li)即(ji)放回(hui)自(zi)(zi)封(feng)袋(dai)中(zhong)(zhong)，密封(feng)（低溫干(gan)燥）保存。濃(nong)度(du)(du)為0的(de)(de)(de)(de)S0號標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)即(ji)可視為陰性(xing)對照或者(zhe)空白；按(an)照說(shuo)明(ming)(ming)書(shu)(shu)操(cao)(cao)作(zuo)(zuo)(zuo)(zuo)時(shi)樣(yang)(yang)本(ben)已經稀(xi)釋5倍，＊終結(jie)果(guo)(guo)乘以(yi)5才是樣(yang)(yang)本(ben)實(shi)際濃(nong)度(du)(du)。嚴格(ge)(ge)按(an)照說(shuo)明(ming)(ming)書(shu)(shu)中(zhong)(zhong)標(biao)(biao)(biao)(biao)明(ming)(ming)的(de)(de)(de)(de)時(shi)間、加(jia)(jia)液(ye)(ye)(ye)(ye)量及(ji)順序進行(xing)溫育(yu)操(cao)(cao)作(zuo)(zuo)(zuo)(zuo)。5. 所有液(ye)(ye)(ye)(ye)體組分(fen)(fen)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前(qian)充分(fen)(fen)搖勻(yun)。試(shi)(shi)(shi)劑(ji)(ji)盒組成(cheng)名稱96孔(kong)配(pei)置48孔(kong)配(pei)置備(bei)(bei)(bei)注(zhu)(zhu)微(wei)孔(kong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)板(ban)12孔(kong)×8條12孔(kong)×4條無(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)0.3mL*6管(guan)0.3mL*6管(guan)無(wu)(wu)(wu)(wu)(wu)(wu)(wu)樣(yang)(yang)本(ben)稀(xi)釋液(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)(wu)(wu)(wu)(wu)(wu)檢(jian)測(ce)(ce)(ce)抗體-HRP10mL5mL無(wu)(wu)(wu)(wu)(wu)(wu)(wu)20×洗(xi)滌(di)緩(huan)(huan)沖(chong)液(ye)(ye)(ye)(ye)25mL15mL按(an)說(shuo)明(ming)(ming)書(shu)(shu)進行(xing)稀(xi)釋底物(wu)(wu)(wu)A6mL3mL無(wu)(wu)(wu)(wu)(wu)(wu)(wu)底物(wu)(wu)(wu)B6mL3mL無(wu)(wu)(wu)(wu)(wu)(wu)(wu)終止液(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)(wu)(wu)(wu)(wu)(wu)封(feng)板(ban)膜(mo)2張(zhang)2張(zhang)無(wu)(wu)(wu)(wu)(wu)(wu)(wu)說(shuo)明(ming)(ming)書(shu)(shu)1份1份無(wu)(wu)(wu)(wu)(wu)(wu)(wu)自(zi)(zi)封(feng)袋(dai)1個(ge)1個(ge)無(wu)(wu)(wu)(wu)(wu)(wu)(wu)注(zhu)(zhu)：標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)（S0-S5）濃(nong)度(du)(du)依次(ci)為：0、30、60、120、240、480 pg/mL試(shi)(shi)(shi)劑(ji)(ji)的(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)備(bei)(bei)(bei) 20×洗(xi)滌(di)緩(huan)(huan)沖(chong)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)稀(xi)釋：蒸(zheng)餾水(shui)按(an)1：20稀(xi)釋，即(ji)1份的(de)(de)(de)(de)20×洗(xi)滌(di)緩(huan)(huan)沖(chong)液(ye)(ye)(ye)(ye)加(jia)(jia)19份的(de)(de)(de)(de)蒸(zheng)餾水(shui)。洗(xi)板(ban)方(fang)(fang)法手工(gong)洗(xi)板(ban)：甩盡(jin)(jin)孔(kong)內液(ye)(ye)(ye)(ye)體，每孔(kong)加(jia)(jia)滿洗(xi)滌(di)液(ye)(ye)(ye)(ye)，靜置1min后甩盡(jin)(jin)孔(kong)內液(ye)(ye)(ye)(ye)體，在(zai)吸(xi)水(shui)紙上(shang)(shang)(shang)拍干(gan)，如此洗(xi)板(ban)5次(ci)。自(zi)(zi)動洗(xi)板(ban)機：每孔(kong)注(zhu)(zhu)入(ru)洗(xi)液(ye)(ye)(ye)(ye)350μL，浸泡1min，洗(xi)板(ban)5次(ci)。操(cao)(cao)作(zuo)(zuo)(zuo)(zuo)步驟(zou) 結(jie)果(guo)(guo)判斷繪制標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)線：在(zai)Excel工(gong)作(zuo)(zuo)(zuo)(zuo)表中(zhong)(zhong)，以(yi)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)濃(nong)度(du)(du)作(zuo)(zuo)(zuo)(zuo)橫坐(zuo)標(biao)(biao)(biao)(biao)，對應(ying)OD值作(zuo)(zuo)(zuo)(zuo)縱坐(zuo)標(biao)(biao)(biao)(biao)，繪制出標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)線性(xing)回(hui)歸(gui)(gui)曲(qu)線，按(an)曲(qu)線方(fang)(fang)程計算各(ge)樣(yang)(yang)本(ben)濃(nong)度(du)(du)值。試(shi)(shi)(shi)劑(ji)(ji)盒性(xing)能準(zhun)(zhun)(zhun)(zhun)確(que)性(xing)：標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)線性(xing)回(hui)歸(gui)(gui)與預(yu)期(qi)濃(nong)度(du)(du)相關系(xi)數R值，大(da)于(yu)等于(yu)0.9900。靈敏(min)度(du)(du)：＊低檢(jian)測(ce)(ce)(ce)濃(nong)度(du)(du)小于(yu)1.0 pg/mL。特異(yi)性(xing)：不(bu)(bu)與其它可溶性(xing)結(jie)構類似物(wu)(wu)(wu)交叉反(fan)應(ying)。重復(fu)性(xing)：板(ban)內、板(ban)間變(bian)異(yi)系(xi)數均小于(yu)15%。貯(zhu)藏：2-8℃，避(bi)光防潮(chao)保存。有效期(qi)：6個(ge)月免責(ze)聲明(ming)(ming)試(shi)(shi)(shi)劑(ji)(ji)盒僅供研(yan)究(jiu)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)，不(bu)(bu)得用(yong)(yong)(yong)(yong)于(yu)臨(lin)床實(shi)驗或人體實(shi)驗，否(fou)則所產生(sheng)的(de)(de)(de)(de)一(yi)切后果(guo)(guo)，由實(shi)驗者(zhe)承擔，本(ben)公司概不(bu)(bu)負(fu)責(ze)。嚴格(ge)(ge)按(an)照說(shuo)明(ming)(ming)書(shu)(shu)操(cao)(cao)作(zuo)(zuo)(zuo)(zuo)，實(shi)驗者(zhe)違反(fan)說(shuo)明(ming)(ming)書(shu)(shu)操(cao)(cao)作(zuo)(zuo)(zuo)(zuo)，后果(guo)(guo)由實(shi)驗者(zhe)承擔。技(ji)(ji)術(shu)支持電話：電子郵件(jian)：網址：fllygs.com生(sheng)產廠家：上(shang)(shang)(shang)海篤瑪生(sheng)物(wu)(wu)(wu)科技(ji)(ji)有限公司
大鼠(shu)（Rat）喹啉(lin)酸(suan)（QUIN）ELISA檢(jian)測(ce)(ce)(ce)(ce)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)本試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)只(zhi)能用(yong)(yong)(yong)于(yu)(yu)科(ke)學研(yan)究，不(bu)(bu)(bu)得用(yong)(yong)(yong)于(yu)(yu)醫學診斷使(shi)(shi)(shi)用(yong)(yong)(yong)說(shuo)(shuo)明(ming)(ming)書產品(pin)(pin)(pin)貨號(hao)：【DM-D9689】產品(pin)(pin)(pin)規格：【48T/96T】檢(jian)測(ce)(ce)(ce)(ce)范圍：反應性(xing)(xing)(xing)：Rat檢(jian)測(ce)(ce)(ce)(ce)范圍：11.37-800 nmol/L靈敏度：＊低(di)檢(jian)測(ce)(ce)(ce)(ce)濃(nong)度小(xiao)于(yu)(yu)10 nmol/L檢(jian)測(ce)(ce)(ce)(ce)原理(li)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)采用(yong)(yong)(yong)雙抗(kang)(kang)體(ti)(ti)一(yi)步夾心法酶(mei)聯免疫(yi)吸(xi)附試(shi)(shi)(shi)驗(yan)（ELISA）。往(wang)預先包被(bei)喹啉(lin)酸(suan)（QUIN）抗(kang)(kang)體(ti)(ti)的(de)(de)(de)包被(bei)微孔(kong)(kong)(kong)(kong)(kong)中，依(yi)次加(jia)入標(biao)(biao)(biao)(biao)(biao)本、標(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)記的(de)(de)(de)檢(jian)測(ce)(ce)(ce)(ce)抗(kang)(kang)體(ti)(ti)，經過溫育(yu)并徹底洗(xi)(xi)(xi)(xi)滌。用(yong)(yong)(yong)底物TMB顯色(se)(se)，TMB在(zai)(zai)過氧化(hua)物酶(mei)的(de)(de)(de)催化(hua)下(xia)轉化(hua)成藍色(se)(se)，并在(zai)(zai)酸(suan)的(de)(de)(de)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)下(xia)轉化(hua)成＊終(zhong)的(de)(de)(de)黃色(se)(se)。顏色(se)(se)的(de)(de)(de)深淺和樣(yang)(yang)(yang)品(pin)(pin)(pin)中的(de)(de)(de)喹啉(lin)酸(suan)（QUIN）呈(cheng)正相關(guan)(guan)。用(yong)(yong)(yong)酶(mei)標(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)450nm 波長下(xia)測(ce)(ce)(ce)(ce)定吸(xi)光度（OD 值），計(ji)算(suan)樣(yang)(yang)(yang)品(pin)(pin)(pin)濃(nong)度。樣(yang)(yang)(yang)品(pin)(pin)(pin)收集(ji)、處理(li)及(ji)保存(cun)方法1. 血清(qing)：使(shi)(shi)(shi)用(yong)(yong)(yong)不(bu)(bu)(bu)含熱原和內(nei)(nei)毒素的(de)(de)(de)試(shi)(shi)(shi)管，操(cao)(cao)作(zuo)(zuo)(zuo)過程中避免任何細胞刺激，收集(ji)血液(ye)(ye)(ye)(ye)后(hou)，3000轉離(li)心10分(fen)(fen)(fen)鐘將血清(qing)和紅(hong)細胞迅速小(xiao)心地分(fen)(fen)(fen)離(li)。2. 血漿(jiang)：EDTA、檸檬酸(suan)鹽或(huo)(huo)肝素抗(kang)(kang)凝。3000轉離(li)心30分(fen)(fen)(fen)鐘取上(shang)清(qing)。3. 細胞上(shang)清(qing)液(ye)(ye)(ye)(ye)：3000轉離(li)心10分(fen)(fen)(fen)鐘去除顆粒和聚合(he)物。4. 組(zu)織勻漿(jiang)：將組(zu)織加(jia)入適量生理(li)鹽水搗碎。3000轉離(li)心10分(fen)(fen)(fen)鐘取上(shang)清(qing)。5. 保存(cun)：如果(guo)樣(yang)(yang)(yang)本收集(ji)后(hou)不(bu)(bu)(bu)及(ji)時檢(jian)測(ce)(ce)(ce)(ce)，請(qing)按(an)(an)一(yi)次用(yong)(yong)(yong)量分(fen)(fen)(fen)裝，凍(dong)存(cun)于(yu)(yu)-20℃，避免反復凍(dong)融，在(zai)(zai)室(shi)(shi)溫下(xia)解凍(dong)并確保樣(yang)(yang)(yang)品(pin)(pin)(pin)均(jun)勻地充(chong)分(fen)(fen)(fen)解凍(dong)。自(zi)(zi)備(bei)(bei)物品(pin)(pin)(pin)酶(mei)標(biao)(biao)(biao)(biao)(biao)儀（450nm）高精度加(jia)樣(yang)(yang)(yang)器(qi)及(ji)槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL37℃恒溫箱操(cao)(cao)作(zuo)(zuo)(zuo)注(zhu)(zhu)意事項試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)保存(cun)在(zai)(zai)2-8℃，使(shi)(shi)(shi)用(yong)(yong)(yong)前室(shi)(shi)溫平衡20分(fen)(fen)(fen)鐘。從冰箱取出的(de)(de)(de)濃(nong)縮洗(xi)(xi)(xi)(xi)滌液(ye)(ye)(ye)(ye)會有結(jie)晶(jing)，這(zhe)屬于(yu)(yu)正常現(xian)象，水浴加(jia)熱使(shi)(shi)(shi)結(jie)晶(jing)完(wan)全(quan)溶(rong)解后(hou)再使(shi)(shi)(shi)用(yong)(yong)(yong)。實(shi)驗(yan)中不(bu)(bu)(bu)用(yong)(yong)(yong)的(de)(de)(de)板(ban)(ban)(ban)條(tiao)應立即放回(hui)自(zi)(zi)封袋中，密封（低(di)溫干燥）保存(cun)。濃(nong)度為0的(de)(de)(de)S0號(hao)標(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)即可(ke)(ke)視為陰(yin)性(xing)(xing)(xing)對(dui)照(zhao)(zhao)或(huo)(huo)者(zhe)空白；按(an)(an)照(zhao)(zhao)說(shuo)(shuo)明(ming)(ming)書操(cao)(cao)作(zuo)(zuo)(zuo)時樣(yang)(yang)(yang)本已經稀(xi)釋5倍，＊終(zhong)結(jie)果(guo)乘(cheng)以5才是樣(yang)(yang)(yang)本實(shi)際(ji)濃(nong)度。嚴格按(an)(an)照(zhao)(zhao)說(shuo)(shuo)明(ming)(ming)書中標(biao)(biao)(biao)(biao)(biao)明(ming)(ming)的(de)(de)(de)時間(jian)、加(jia)液(ye)(ye)(ye)(ye)量及(ji)順(shun)序進(jin)行溫育(yu)操(cao)(cao)作(zuo)(zuo)(zuo)。5. 所有液(ye)(ye)(ye)(ye)體(ti)(ti)組(zu)分(fen)(fen)(fen)使(shi)(shi)(shi)用(yong)(yong)(yong)前充(chong)分(fen)(fen)(fen)搖勻。試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)組(zu)成名稱96孔(kong)(kong)(kong)(kong)(kong)配(pei)置48孔(kong)(kong)(kong)(kong)(kong)配(pei)置備(bei)(bei)注(zhu)(zhu)微孔(kong)(kong)(kong)(kong)(kong)酶(mei)標(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)12孔(kong)(kong)(kong)(kong)(kong)×8條(tiao)12孔(kong)(kong)(kong)(kong)(kong)×4條(tiao)無標(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)0.3mL*6管0.3mL*6管無樣(yang)(yang)(yang)本稀(xi)釋液(ye)(ye)(ye)(ye)6mL3mL無檢(jian)測(ce)(ce)(ce)(ce)抗(kang)(kang)體(ti)(ti)-HRP10mL5mL無20×洗(xi)(xi)(xi)(xi)滌緩(huan)沖(chong)液(ye)(ye)(ye)(ye)25mL15mL按(an)(an)說(shuo)(shuo)明(ming)(ming)書進(jin)行稀(xi)釋底物A6mL3mL無底物B6mL3mL無終(zhong)止(zhi)液(ye)(ye)(ye)(ye)6mL3mL無封板(ban)(ban)(ban)膜2張2張無說(shuo)(shuo)明(ming)(ming)書1份(fen)1份(fen)無自(zi)(zi)封袋1個(ge)1個(ge)無注(zhu)(zhu)：標(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)（S0-S5）濃(nong)度依(yi)次為：0、50、100、200、400、800 nmol/L試(shi)(shi)(shi)劑(ji)(ji)(ji)的(de)(de)(de)準(zhun)備(bei)(bei) 20×洗(xi)(xi)(xi)(xi)滌緩(huan)沖(chong)液(ye)(ye)(ye)(ye)的(de)(de)(de)稀(xi)釋：蒸(zheng)餾(liu)水按(an)(an)1：20稀(xi)釋，即1份(fen)的(de)(de)(de)20×洗(xi)(xi)(xi)(xi)滌緩(huan)沖(chong)液(ye)(ye)(ye)(ye)加(jia)19份(fen)的(de)(de)(de)蒸(zheng)餾(liu)水。洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)方法手工洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)：甩盡(jin)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)體(ti)(ti)，每孔(kong)(kong)(kong)(kong)(kong)加(jia)滿洗(xi)(xi)(xi)(xi)滌液(ye)(ye)(ye)(ye)，靜(jing)置1min后(hou)甩盡(jin)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)體(ti)(ti)，在(zai)(zai)吸(xi)水紙(zhi)上(shang)拍干，如此洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)5次。自(zi)(zi)動(dong)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)機：每孔(kong)(kong)(kong)(kong)(kong)注(zhu)(zhu)入洗(xi)(xi)(xi)(xi)液(ye)(ye)(ye)(ye)350μL，浸(jin)泡1min，洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)5次。操(cao)(cao)作(zuo)(zuo)(zuo)步驟 結(jie)果(guo)判斷繪(hui)制(zhi)標(biao)(biao)(biao)(biao)(biao)準(zhun)曲(qu)線：在(zai)(zai)Excel工作(zuo)(zuo)(zuo)表中，以標(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)濃(nong)度作(zuo)(zuo)(zuo)橫坐標(biao)(biao)(biao)(biao)(biao)，對(dui)應OD值作(zuo)(zuo)(zuo)縱坐標(biao)(biao)(biao)(biao)(biao)，繪(hui)制(zhi)出標(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)線性(xing)(xing)(xing)回(hui)歸曲(qu)線，按(an)(an)曲(qu)線方程計(ji)算(suan)各樣(yang)(yang)(yang)本濃(nong)度值。試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)性(xing)(xing)(xing)能準(zhun)確性(xing)(xing)(xing)：標(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)線性(xing)(xing)(xing)回(hui)歸與預期(qi)濃(nong)度相關(guan)(guan)系數(shu)R值，大于(yu)(yu)等(deng)于(yu)(yu)0.9900。靈敏度：＊低(di)檢(jian)測(ce)(ce)(ce)(ce)濃(nong)度小(xiao)于(yu)(yu)1.0 pg/mL。特異(yi)性(xing)(xing)(xing)：不(bu)(bu)(bu)與其它可(ke)(ke)溶(rong)性(xing)(xing)(xing)結(jie)構類(lei)似物交叉反應。重復性(xing)(xing)(xing)：板(ban)(ban)(ban)內(nei)(nei)、板(ban)(ban)(ban)間(jian)變異(yi)系數(shu)均(jun)小(xiao)于(yu)(yu)15%。貯藏：2-8℃，避光防潮保存(cun)。有效(xiao)期(qi)：6個(ge)月免責聲(sheng)明(ming)(ming)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)僅供研(yan)究使(shi)(shi)(shi)用(yong)(yong)(yong)，不(bu)(bu)(bu)得用(yong)(yong)(yong)于(yu)(yu)臨床實(shi)驗(yan)或(huo)(huo)人體(ti)(ti)實(shi)驗(yan)，否(fou)則所產生的(de)(de)(de)一(yi)切(qie)后(hou)果(guo)，由(you)實(shi)驗(yan)者(zhe)承擔(dan)，本公司概不(bu)(bu)(bu)負責。嚴格按(an)(an)照(zhao)(zhao)說(shuo)(shuo)明(ming)(ming)書操(cao)(cao)作(zuo)(zuo)(zuo)，實(shi)驗(yan)者(zhe)違反說(shuo)(shuo)明(ming)(ming)書操(cao)(cao)作(zuo)(zuo)(zuo)，后(hou)果(guo)由(you)實(shi)驗(yan)者(zhe)承擔(dan)。技術支持電話：電子郵件：網(wang)址：fllygs.com生產廠家：上(shang)海篤瑪生物科(ke)技有限公司
大(da)鼠（Rat）5羥(qian)色(se)胺(an)（5-HT）ELISA檢測(ce)試劑盒(he)(he)本試劑盒(he)(he)只(zhi)能(neng)用(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科學研(yan)(yan)究(jiu)，不(bu)(bu)(bu)得用(yong)(yong)于(yu)(yu)(yu)(yu)(yu)醫學診斷使(shi)用(yong)(yong)說(shuo)明(ming)書(shu)產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)貨號：【DM-D9688】產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)規格(ge)：【48T/96T】檢測(ce)范(fan)圍：反應(ying)性(xing)：Rat檢測(ce)范(fan)圍：6.43-480 ng/mL靈敏度(du)(du)(du)(du)(du)(du)： ＊低檢測(ce)濃(nong)度(du)(du)(du)(du)(du)(du)小于(yu)(yu)(yu)(yu)(yu)1.0 ng/mL檢測(ce)原(yuan)理(li)(li)試劑盒(he)(he)采用(yong)(yong)雙抗(kang)(kang)體(ti)(ti)一(yi)步(bu)夾心(xin)(xin)法酶(mei)(mei)聯(lian)免疫吸附試驗(yan)(yan)（ELISA）。往預先包被(bei)5羥(qian)色(se)胺(an)（5-HT）抗(kang)(kang)體(ti)(ti)的(de)(de)(de)(de)包被(bei)微(wei)(wei)孔(kong)中，依(yi)次(ci)加入標(biao)(biao)(biao)(biao)本、標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)檢測(ce)抗(kang)(kang)體(ti)(ti)，經過(guo)溫育并徹底(di)洗(xi)(xi)滌(di)(di)。用(yong)(yong)底(di)物(wu)(wu)TMB顯色(se)，TMB在(zai)過(guo)氧化(hua)(hua)物(wu)(wu)酶(mei)(mei)的(de)(de)(de)(de)催化(hua)(hua)下(xia)(xia)轉(zhuan)化(hua)(hua)成藍色(se)，并在(zai)酸的(de)(de)(de)(de)作(zuo)用(yong)(yong)下(xia)(xia)轉(zhuan)化(hua)(hua)成＊終的(de)(de)(de)(de)黃色(se)。顏色(se)的(de)(de)(de)(de)深淺和樣(yang)品(pin)(pin)(pin)(pin)中的(de)(de)(de)(de)5羥(qian)色(se)胺(an)（5-HT）呈正(zheng)相關。用(yong)(yong)酶(mei)(mei)標(biao)(biao)(biao)(biao)儀在(zai)450nm 波長(chang)下(xia)(xia)測(ce)定吸光度(du)(du)(du)(du)(du)(du)（OD 值），計算樣(yang)品(pin)(pin)(pin)(pin)濃(nong)度(du)(du)(du)(du)(du)(du)。樣(yang)品(pin)(pin)(pin)(pin)收集、處理(li)(li)及保(bao)(bao)存(cun)方法1. 血清(qing)：使(shi)用(yong)(yong)不(bu)(bu)(bu)含熱(re)原(yuan)和內毒素(su)的(de)(de)(de)(de)試管(guan)，操(cao)(cao)(cao)作(zuo)過(guo)程(cheng)(cheng)中避免任何(he)細胞(bao)刺激，收集血液(ye)(ye)(ye)(ye)后，3000轉(zhuan)離(li)(li)心(xin)(xin)10分鐘將血清(qing)和紅(hong)細胞(bao)迅速(su)小心(xin)(xin)地(di)分離(li)(li)。2. 血漿(jiang)(jiang)：EDTA、檸(ning)檬酸鹽或肝(gan)素(su)抗(kang)(kang)凝(ning)。3000轉(zhuan)離(li)(li)心(xin)(xin)30分鐘取上(shang)(shang)清(qing)。3. 細胞(bao)上(shang)(shang)清(qing)液(ye)(ye)(ye)(ye)：3000轉(zhuan)離(li)(li)心(xin)(xin)10分鐘去除顆粒和聚(ju)合物(wu)(wu)。4. 組(zu)織(zhi)勻(yun)漿(jiang)(jiang)：將組(zu)織(zhi)加入適量生(sheng)理(li)(li)鹽水(shui)搗碎。3000轉(zhuan)離(li)(li)心(xin)(xin)10分鐘取上(shang)(shang)清(qing)。5. 保(bao)(bao)存(cun)：如果(guo)樣(yang)本收集后不(bu)(bu)(bu)及時(shi)檢測(ce)，請按(an)(an)一(yi)次(ci)用(yong)(yong)量分裝，凍(dong)(dong)存(cun)于(yu)(yu)(yu)(yu)(yu)-20℃，避免反復(fu)(fu)凍(dong)(dong)融，在(zai)室(shi)溫下(xia)(xia)解凍(dong)(dong)并確保(bao)(bao)樣(yang)品(pin)(pin)(pin)(pin)均勻(yun)地(di)充分解凍(dong)(dong)。自備物(wu)(wu)品(pin)(pin)(pin)(pin)酶(mei)(mei)標(biao)(biao)(biao)(biao)儀（450nm）高(gao)精(jing)度(du)(du)(du)(du)(du)(du)加樣(yang)器及槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL37℃恒溫箱(xiang)操(cao)(cao)(cao)作(zuo)注(zhu)意(yi)事項試劑盒(he)(he)保(bao)(bao)存(cun)在(zai)2-8℃，使(shi)用(yong)(yong)前室(shi)溫平衡20分鐘。從冰箱(xiang)取出(chu)的(de)(de)(de)(de)濃(nong)縮洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)會有結晶(jing)，這屬(shu)于(yu)(yu)(yu)(yu)(yu)正(zheng)常(chang)現象(xiang)，水(shui)浴(yu)加熱(re)使(shi)結晶(jing)完全溶解后再(zai)使(shi)用(yong)(yong)。實驗(yan)(yan)中不(bu)(bu)(bu)用(yong)(yong)的(de)(de)(de)(de)板(ban)(ban)(ban)(ban)(ban)條應(ying)立即放回(hui)自封袋中，密封（低溫干(gan)燥）保(bao)(bao)存(cun)。濃(nong)度(du)(du)(du)(du)(du)(du)為0的(de)(de)(de)(de)S0號標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)即可視為陰性(xing)對照或者(zhe)(zhe)空白；按(an)(an)照說(shuo)明(ming)書(shu)操(cao)(cao)(cao)作(zuo)時(shi)樣(yang)本已經稀(xi)(xi)釋(shi)(shi)(shi)5倍，＊終結果(guo)乘以(yi)5才(cai)是樣(yang)本實際濃(nong)度(du)(du)(du)(du)(du)(du)。嚴(yan)格(ge)按(an)(an)照說(shuo)明(ming)書(shu)中標(biao)(biao)(biao)(biao)明(ming)的(de)(de)(de)(de)時(shi)間、加液(ye)(ye)(ye)(ye)量及順序進行(xing)溫育操(cao)(cao)(cao)作(zuo)。5. 所有液(ye)(ye)(ye)(ye)體(ti)(ti)組(zu)分使(shi)用(yong)(yong)前充分搖(yao)勻(yun)。試劑盒(he)(he)組(zu)成名稱96孔(kong)配置48孔(kong)配置備注(zhu)微(wei)(wei)孔(kong)酶(mei)(mei)標(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)12孔(kong)×8條12孔(kong)×4條無(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)0.3mL*6管(guan)0.3mL*6管(guan)無(wu)(wu)(wu)(wu)樣(yang)本稀(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)(wu)(wu)檢測(ce)抗(kang)(kang)體(ti)(ti)-HRP10mL5mL無(wu)(wu)(wu)(wu)20×洗(xi)(xi)滌(di)(di)緩(huan)沖液(ye)(ye)(ye)(ye)25mL15mL按(an)(an)說(shuo)明(ming)書(shu)進行(xing)稀(xi)(xi)釋(shi)(shi)(shi)底(di)物(wu)(wu)A6mL3mL無(wu)(wu)(wu)(wu)底(di)物(wu)(wu)B6mL3mL無(wu)(wu)(wu)(wu)終止(zhi)液(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)(wu)(wu)封板(ban)(ban)(ban)(ban)(ban)膜2張(zhang)(zhang)2張(zhang)(zhang)無(wu)(wu)(wu)(wu)說(shuo)明(ming)書(shu)1份1份無(wu)(wu)(wu)(wu)自封袋1個1個無(wu)(wu)(wu)(wu)注(zhu)：標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)（S0-S5）濃(nong)度(du)(du)(du)(du)(du)(du)依(yi)次(ci)為：0、30、60、120、240、480 ng/mL試劑的(de)(de)(de)(de)準(zhun)(zhun)備 20×洗(xi)(xi)滌(di)(di)緩(huan)沖液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)稀(xi)(xi)釋(shi)(shi)(shi)：蒸餾水(shui)按(an)(an)1：20稀(xi)(xi)釋(shi)(shi)(shi)，即1份的(de)(de)(de)(de)20×洗(xi)(xi)滌(di)(di)緩(huan)沖液(ye)(ye)(ye)(ye)加19份的(de)(de)(de)(de)蒸餾水(shui)。洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)方法手工洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)：甩盡孔(kong)內液(ye)(ye)(ye)(ye)體(ti)(ti)，每(mei)孔(kong)加滿洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)，靜置1min后甩盡孔(kong)內液(ye)(ye)(ye)(ye)體(ti)(ti)，在(zai)吸水(shui)紙上(shang)(shang)拍(pai)干(gan)，如此洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)5次(ci)。自動洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)機：每(mei)孔(kong)注(zhu)入洗(xi)(xi)液(ye)(ye)(ye)(ye)350μL，浸泡1min，洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)5次(ci)。操(cao)(cao)(cao)作(zuo)步(bu)驟 結果(guo)判斷繪制(zhi)標(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)線：在(zai)Excel工作(zuo)表(biao)中，以(yi)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)濃(nong)度(du)(du)(du)(du)(du)(du)作(zuo)橫坐(zuo)標(biao)(biao)(biao)(biao)，對應(ying)OD值作(zuo)縱坐(zuo)標(biao)(biao)(biao)(biao)，繪制(zhi)出(chu)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)線性(xing)回(hui)歸曲(qu)線，按(an)(an)曲(qu)線方程(cheng)(cheng)計算各樣(yang)本濃(nong)度(du)(du)(du)(du)(du)(du)值。試劑盒(he)(he)性(xing)能(neng)準(zhun)(zhun)確性(xing)：標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)線性(xing)回(hui)歸與(yu)預期(qi)濃(nong)度(du)(du)(du)(du)(du)(du)相關系(xi)數(shu)R值，大(da)于(yu)(yu)(yu)(yu)(yu)等于(yu)(yu)(yu)(yu)(yu)0.9900。靈敏度(du)(du)(du)(du)(du)(du)：＊低檢測(ce)濃(nong)度(du)(du)(du)(du)(du)(du)小于(yu)(yu)(yu)(yu)(yu)1.0 pg/mL。特異性(xing)：不(bu)(bu)(bu)與(yu)其它可溶性(xing)結構(gou)類(lei)似(si)物(wu)(wu)交(jiao)叉反應(ying)。重復(fu)(fu)性(xing)：板(ban)(ban)(ban)(ban)(ban)內、板(ban)(ban)(ban)(ban)(ban)間變異系(xi)數(shu)均小于(yu)(yu)(yu)(yu)(yu)15%。貯藏：2-8℃，避光防潮保(bao)(bao)存(cun)。有效期(qi)：6個月(yue)免責(ze)聲明(ming)試劑盒(he)(he)僅(jin)供研(yan)(yan)究(jiu)使(shi)用(yong)(yong)，不(bu)(bu)(bu)得用(yong)(yong)于(yu)(yu)(yu)(yu)(yu)臨床實驗(yan)(yan)或人體(ti)(ti)實驗(yan)(yan)，否則(ze)所產(chan)(chan)(chan)生(sheng)的(de)(de)(de)(de)一(yi)切后果(guo)，由實驗(yan)(yan)者(zhe)(zhe)承擔(dan)，本公司概不(bu)(bu)(bu)負責(ze)。嚴(yan)格(ge)按(an)(an)照說(shuo)明(ming)書(shu)操(cao)(cao)(cao)作(zuo)，實驗(yan)(yan)者(zhe)(zhe)違(wei)反說(shuo)明(ming)書(shu)操(cao)(cao)(cao)作(zuo)，后果(guo)由實驗(yan)(yan)者(zhe)(zhe)承擔(dan)。技術支持電話：電子郵件(jian)：網址(zhi)：fllygs.com生(sheng)產(chan)(chan)(chan)廠家：上(shang)(shang)海篤(du)瑪生(sheng)物(wu)(wu)科技有限公司
人（Human）色(se)(se)(se)氨(an)酸(suan)（TRP）ELISA檢測(ce)(ce)試(shi)(shi)(shi)(shi)劑(ji)盒(he)本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)盒(he)只能用(yong)(yong)于(yu)(yu)科學研究(jiu)，不(bu)得(de)用(yong)(yong)于(yu)(yu)醫學診斷(duan)使用(yong)(yong)說(shuo)明(ming)(ming)(ming)(ming)(ming)書產品(pin)(pin)貨(huo)號：【DM6993】產品(pin)(pin)規格：【48T/96T】檢測(ce)(ce)范(fan)圍(wei)：反(fan)應性(xing)(xing)：Human檢測(ce)(ce)范(fan)圍(wei)：4.56-480 pg/mL靈(ling)敏度(du)(du)： 1.0 pg/mL檢測(ce)(ce)原(yuan)理(li)試(shi)(shi)(shi)(shi)劑(ji)盒(he)采用(yong)(yong)雙抗體一步(bu)夾心法(fa)(fa)酶(mei)聯(lian)免(mian)(mian)疫(yi)吸(xi)附(fu)試(shi)(shi)(shi)(shi)驗(yan)(yan)（ELISA）。往預先包被色(se)(se)(se)氨(an)酸(suan)（TRP）抗體的(de)(de)包被微(wei)孔(kong)(kong)(kong)中，依次(ci)加入標(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)記的(de)(de)檢測(ce)(ce)抗體，經(jing)過溫(wen)育(yu)(yu)并(bing)徹底(di)洗(xi)(xi)滌(di)(di)。用(yong)(yong)底(di)物TMB顯色(se)(se)(se)，TMB在(zai)過氧化物酶(mei)的(de)(de)催化下(xia)轉(zhuan)化成(cheng)藍色(se)(se)(se)，并(bing)在(zai)酸(suan)的(de)(de)作(zuo)(zuo)用(yong)(yong)下(xia)轉(zhuan)化成(cheng)＊終的(de)(de)黃色(se)(se)(se)。顏色(se)(se)(se)的(de)(de)深(shen)淺和(he)(he)樣(yang)(yang)(yang)品(pin)(pin)中的(de)(de)色(se)(se)(se)氨(an)酸(suan)（TRP）呈正相關。用(yong)(yong)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)在(zai)450nm 波長下(xia)測(ce)(ce)定吸(xi)光度(du)(du)（OD 值(zhi)），計(ji)算樣(yang)(yang)(yang)品(pin)(pin)濃(nong)(nong)度(du)(du)。樣(yang)(yang)(yang)品(pin)(pin)收集、處理(li)及(ji)(ji)保(bao)存(cun)方法(fa)(fa)1. 血(xue)清：使用(yong)(yong)不(bu)含(han)熱原(yuan)和(he)(he)內(nei)毒素的(de)(de)試(shi)(shi)(shi)(shi)管(guan)，操(cao)(cao)(cao)(cao)作(zuo)(zuo)過程(cheng)中避免(mian)(mian)任何細胞刺激，收集血(xue)液(ye)(ye)后(hou)，3000轉(zhuan)離(li)心10分(fen)鐘(zhong)(zhong)將血(xue)清和(he)(he)紅細胞迅速(su)小心地分(fen)離(li)。2. 血(xue)漿：EDTA、檸檬(meng)酸(suan)鹽或(huo)肝素抗凝。3000轉(zhuan)離(li)心30分(fen)鐘(zhong)(zhong)取上清。3. 細胞上清液(ye)(ye)：3000轉(zhuan)離(li)心10分(fen)鐘(zhong)(zhong)去除(chu)顆粒和(he)(he)聚(ju)合物。4. 組(zu)織勻漿：將組(zu)織加入適(shi)量(liang)生(sheng)理(li)鹽水(shui)(shui)(shui)搗碎。3000轉(zhuan)離(li)心10分(fen)鐘(zhong)(zhong)取上清。5. 保(bao)存(cun)：如果樣(yang)(yang)(yang)本(ben)(ben)(ben)收集后(hou)不(bu)及(ji)(ji)時(shi)檢測(ce)(ce)，請(qing)按(an)(an)一次(ci)用(yong)(yong)量(liang)分(fen)裝，凍存(cun)于(yu)(yu)-20℃，避免(mian)(mian)反(fan)復凍融，在(zai)室溫(wen)下(xia)解凍并(bing)確保(bao)樣(yang)(yang)(yang)品(pin)(pin)均(jun)勻地充(chong)(chong)分(fen)解凍。自(zi)備(bei)物品(pin)(pin)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)（450nm）高(gao)精度(du)(du)加樣(yang)(yang)(yang)器及(ji)(ji)槍頭(tou)：0.5-10uL、2-20uL、20-200uL、200-1000uL37℃恒(heng)溫(wen)箱操(cao)(cao)(cao)(cao)作(zuo)(zuo)注意事項(xiang)試(shi)(shi)(shi)(shi)劑(ji)盒(he)保(bao)存(cun)在(zai)2-8℃，使用(yong)(yong)前(qian)室溫(wen)平(ping)衡(heng)20分(fen)鐘(zhong)(zhong)。從冰箱取出的(de)(de)濃(nong)(nong)縮洗(xi)(xi)滌(di)(di)液(ye)(ye)會有(you)結(jie)晶，這屬(shu)于(yu)(yu)正常現象，水(shui)(shui)(shui)浴(yu)加熱使結(jie)晶完全溶解后(hou)再使用(yong)(yong)。實(shi)(shi)驗(yan)(yan)中不(bu)用(yong)(yong)的(de)(de)板(ban)(ban)(ban)(ban)條(tiao)應立即放回(hui)自(zi)封袋(dai)中，密封（低溫(wen)干(gan)燥）保(bao)存(cun)。濃(nong)(nong)度(du)(du)為(wei)0的(de)(de)S0號標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)即可(ke)視為(wei)陰性(xing)(xing)對(dui)照或(huo)者空(kong)白；按(an)(an)照說(shuo)明(ming)(ming)(ming)(ming)(ming)書操(cao)(cao)(cao)(cao)作(zuo)(zuo)時(shi)樣(yang)(yang)(yang)本(ben)(ben)(ben)已經(jing)稀釋(shi)5倍，＊終結(jie)果乘以(yi)5才是樣(yang)(yang)(yang)本(ben)(ben)(ben)實(shi)(shi)際(ji)濃(nong)(nong)度(du)(du)。嚴(yan)格按(an)(an)照說(shuo)明(ming)(ming)(ming)(ming)(ming)書中標(biao)(biao)(biao)(biao)(biao)(biao)明(ming)(ming)(ming)(ming)(ming)的(de)(de)時(shi)間、加液(ye)(ye)量(liang)及(ji)(ji)順序進(jin)行溫(wen)育(yu)(yu)操(cao)(cao)(cao)(cao)作(zuo)(zuo)。5. 所有(you)液(ye)(ye)體組(zu)分(fen)使用(yong)(yong)前(qian)充(chong)(chong)分(fen)搖勻。試(shi)(shi)(shi)(shi)劑(ji)盒(he)組(zu)成(cheng)名稱96孔(kong)(kong)(kong)配置48孔(kong)(kong)(kong)配置備(bei)注微(wei)孔(kong)(kong)(kong)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)12孔(kong)(kong)(kong)×8條(tiao)12孔(kong)(kong)(kong)×4條(tiao)無(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)0.3mL*6管(guan)0.3mL*6管(guan)無(wu)(wu)(wu)樣(yang)(yang)(yang)本(ben)(ben)(ben)稀釋(shi)液(ye)(ye)6mL3mL無(wu)(wu)(wu)檢測(ce)(ce)抗體-HRP10mL5mL無(wu)(wu)(wu)20×洗(xi)(xi)滌(di)(di)緩(huan)(huan)沖液(ye)(ye)25mL15mL按(an)(an)說(shuo)明(ming)(ming)(ming)(ming)(ming)書進(jin)行稀釋(shi)底(di)物A6mL3mL無(wu)(wu)(wu)底(di)物B6mL3mL無(wu)(wu)(wu)終止液(ye)(ye)6mL3mL無(wu)(wu)(wu)封板(ban)(ban)(ban)(ban)膜(mo)2張2張無(wu)(wu)(wu)說(shuo)明(ming)(ming)(ming)(ming)(ming)書1份1份無(wu)(wu)(wu)自(zi)封袋(dai)1個1個無(wu)(wu)(wu)注：標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)（S0-S5）濃(nong)(nong)度(du)(du)依次(ci)為(wei)：0、30、60、120、240、480 pg/mL試(shi)(shi)(shi)(shi)劑(ji)的(de)(de)準(zhun)備(bei) 20×洗(xi)(xi)滌(di)(di)緩(huan)(huan)沖液(ye)(ye)的(de)(de)稀釋(shi)：蒸(zheng)餾(liu)水(shui)(shui)(shui)按(an)(an)1：20稀釋(shi)，即1份的(de)(de)20×洗(xi)(xi)滌(di)(di)緩(huan)(huan)沖液(ye)(ye)加19份的(de)(de)蒸(zheng)餾(liu)水(shui)(shui)(shui)。洗(xi)(xi)板(ban)(ban)(ban)(ban)方法(fa)(fa)手工洗(xi)(xi)板(ban)(ban)(ban)(ban)：甩盡(jin)(jin)孔(kong)(kong)(kong)內(nei)液(ye)(ye)體，每孔(kong)(kong)(kong)加滿(man)洗(xi)(xi)滌(di)(di)液(ye)(ye)，靜置1min后(hou)甩盡(jin)(jin)孔(kong)(kong)(kong)內(nei)液(ye)(ye)體，在(zai)吸(xi)水(shui)(shui)(shui)紙上拍干(gan)，如此洗(xi)(xi)板(ban)(ban)(ban)(ban)5次(ci)。自(zi)動洗(xi)(xi)板(ban)(ban)(ban)(ban)機：每孔(kong)(kong)(kong)注入洗(xi)(xi)液(ye)(ye)350μL，浸泡1min，洗(xi)(xi)板(ban)(ban)(ban)(ban)5次(ci)。操(cao)(cao)(cao)(cao)作(zuo)(zuo)步(bu)驟 結(jie)果判斷(duan)繪制標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)曲(qu)線(xian)：在(zai)Excel工作(zuo)(zuo)表中，以(yi)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)濃(nong)(nong)度(du)(du)作(zuo)(zuo)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)，對(dui)應OD值(zhi)作(zuo)(zuo)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)，繪制出標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)線(xian)性(xing)(xing)回(hui)歸(gui)(gui)曲(qu)線(xian)，按(an)(an)曲(qu)線(xian)方程(cheng)計(ji)算各樣(yang)(yang)(yang)本(ben)(ben)(ben)濃(nong)(nong)度(du)(du)值(zhi)。試(shi)(shi)(shi)(shi)劑(ji)盒(he)性(xing)(xing)能準(zhun)確性(xing)(xing)：標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)線(xian)性(xing)(xing)回(hui)歸(gui)(gui)與預期濃(nong)(nong)度(du)(du)相關系(xi)(xi)數(shu)(shu)R值(zhi)，大于(yu)(yu)等(deng)于(yu)(yu)0.9900。靈(ling)敏度(du)(du)：＊低檢測(ce)(ce)濃(nong)(nong)度(du)(du)小于(yu)(yu)1.0 pg/mL。特異性(xing)(xing)：不(bu)與其它可(ke)溶性(xing)(xing)結(jie)構類似物交叉反(fan)應。重復性(xing)(xing)：板(ban)(ban)(ban)(ban)內(nei)、板(ban)(ban)(ban)(ban)間變異系(xi)(xi)數(shu)(shu)均(jun)小于(yu)(yu)15%。貯(zhu)藏：2-8℃，避光防(fang)潮(chao)保(bao)存(cun)。有(you)效期：6個月免(mian)(mian)責聲明(ming)(ming)(ming)(ming)(ming)試(shi)(shi)(shi)(shi)劑(ji)盒(he)僅供研究(jiu)使用(yong)(yong)，不(bu)得(de)用(yong)(yong)于(yu)(yu)臨(lin)床實(shi)(shi)驗(yan)(yan)或(huo)人體實(shi)(shi)驗(yan)(yan)，否(fou)則所產生(sheng)的(de)(de)一切(qie)后(hou)果，由實(shi)(shi)驗(yan)(yan)者承(cheng)擔(dan)，本(ben)(ben)(ben)公司(si)概不(bu)負責。嚴(yan)格按(an)(an)照說(shuo)明(ming)(ming)(ming)(ming)(ming)書操(cao)(cao)(cao)(cao)作(zuo)(zuo)，實(shi)(shi)驗(yan)(yan)者違(wei)反(fan)說(shuo)明(ming)(ming)(ming)(ming)(ming)書操(cao)(cao)(cao)(cao)作(zuo)(zuo)，后(hou)果由實(shi)(shi)驗(yan)(yan)者承(cheng)擔(dan)。技術支持電話：電子郵(you)件：網(wang)址：fllygs.com生(sheng)產廠家：上海(hai)篤瑪(ma)生(sheng)物科技有(you)限公司(si)
犬（Canine）5羥色(se)胺(an)（5-HT）ELISA檢(jian)測(ce)(ce)(ce)(ce)試(shi)(shi)(shi)劑(ji)(ji)盒(he)本(ben)試(shi)(shi)(shi)劑(ji)(ji)盒(he)只(zhi)能(neng)(neng)用(yong)(yong)于(yu)(yu)(yu)(yu)科學(xue)研究，不(bu)得(de)(de)用(yong)(yong)于(yu)(yu)(yu)(yu)醫學(xue)診斷(duan)使用(yong)(yong)說明(ming)(ming)書(shu)產(chan)品(pin)(pin)(pin)(pin)貨號(hao)：【DM-Can45213】產(chan)品(pin)(pin)(pin)(pin)規(gui)格：【48T/96T】檢(jian)測(ce)(ce)(ce)(ce)范(fan)圍：反(fan)應性(xing)：Canine檢(jian)測(ce)(ce)(ce)(ce)范(fan)圍：6.43-480 ng/mL靈敏度(du)： ＊低(di)檢(jian)測(ce)(ce)(ce)(ce)濃(nong)度(du)小于(yu)(yu)(yu)(yu)1.0 ng/mL檢(jian)測(ce)(ce)(ce)(ce)原理試(shi)(shi)(shi)劑(ji)(ji)盒(he)采用(yong)(yong)雙(shuang)抗(kang)體(ti)一步夾(jia)心(xin)法(fa)酶(mei)聯(lian)免(mian)疫吸附試(shi)(shi)(shi)驗(yan)（ELISA）。往預先(xian)包(bao)被5羥色(se)胺(an)（5-HT）抗(kang)體(ti)的(de)(de)(de)(de)包(bao)被微孔(kong)(kong)中，依次加入標(biao)(biao)(biao)(biao)本(ben)、標(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)檢(jian)測(ce)(ce)(ce)(ce)抗(kang)體(ti)，經過(guo)(guo)(guo)溫育并徹底洗滌(di)(di)(di)。用(yong)(yong)底物(wu)(wu)(wu)TMB顯色(se)，TMB在(zai)(zai)過(guo)(guo)(guo)氧化物(wu)(wu)(wu)酶(mei)的(de)(de)(de)(de)催化下轉化成(cheng)藍色(se)，并在(zai)(zai)酸(suan)的(de)(de)(de)(de)作用(yong)(yong)下轉化成(cheng)＊終的(de)(de)(de)(de)黃(huang)色(se)。顏色(se)的(de)(de)(de)(de)深淺和(he)(he)(he)(he)樣(yang)品(pin)(pin)(pin)(pin)中的(de)(de)(de)(de)5羥色(se)胺(an)（5-HT）呈正相(xiang)關。用(yong)(yong)酶(mei)標(biao)(biao)(biao)(biao)儀(yi)在(zai)(zai)450nm 波長下測(ce)(ce)(ce)(ce)定吸光度(du)（OD 值），計算(suan)樣(yang)品(pin)(pin)(pin)(pin)濃(nong)度(du)。樣(yang)品(pin)(pin)(pin)(pin)收集、處理及保(bao)(bao)存方法(fa)1. 血(xue)清(qing)(qing)：使用(yong)(yong)不(bu)含熱(re)(re)原和(he)(he)(he)(he)內(nei)毒素的(de)(de)(de)(de)試(shi)(shi)(shi)管，操(cao)作過(guo)(guo)(guo)程中避(bi)免(mian)任何細胞(bao)刺(ci)激(ji)，收集血(xue)液(ye)(ye)(ye)(ye)后(hou)，3000轉離(li)(li)(li)心(xin)10分(fen)(fen)鐘將血(xue)清(qing)(qing)和(he)(he)(he)(he)紅細胞(bao)迅速小心(xin)地(di)分(fen)(fen)離(li)(li)(li)。2. 血(xue)漿：EDTA、檸檬酸(suan)鹽(yan)或肝素抗(kang)凝。3000轉離(li)(li)(li)心(xin)30分(fen)(fen)鐘取(qu)上(shang)(shang)清(qing)(qing)。3. 細胞(bao)上(shang)(shang)清(qing)(qing)液(ye)(ye)(ye)(ye)：3000轉離(li)(li)(li)心(xin)10分(fen)(fen)鐘去除顆粒和(he)(he)(he)(he)聚(ju)合物(wu)(wu)(wu)。4. 組(zu)織勻(yun)漿：將組(zu)織加入適量(liang)(liang)生理鹽(yan)水(shui)搗碎。3000轉離(li)(li)(li)心(xin)10分(fen)(fen)鐘取(qu)上(shang)(shang)清(qing)(qing)。5. 保(bao)(bao)存：如果樣(yang)本(ben)收集后(hou)不(bu)及時檢(jian)測(ce)(ce)(ce)(ce)，請按(an)(an)(an)一次用(yong)(yong)量(liang)(liang)分(fen)(fen)裝，凍(dong)存于(yu)(yu)(yu)(yu)-20℃，避(bi)免(mian)反(fan)復凍(dong)融，在(zai)(zai)室溫下解(jie)凍(dong)并確保(bao)(bao)樣(yang)品(pin)(pin)(pin)(pin)均(jun)(jun)勻(yun)地(di)充分(fen)(fen)解(jie)凍(dong)。自備物(wu)(wu)(wu)品(pin)(pin)(pin)(pin)酶(mei)標(biao)(biao)(biao)(biao)儀(yi)（450nm）高精(jing)度(du)加樣(yang)器及槍(qiang)頭(tou)：0.5-10uL、2-20uL、20-200uL、200-1000uL37℃恒(heng)溫箱操(cao)作注(zhu)(zhu)意事項試(shi)(shi)(shi)劑(ji)(ji)盒(he)保(bao)(bao)存在(zai)(zai)2-8℃，使用(yong)(yong)前室溫平衡(heng)20分(fen)(fen)鐘。從(cong)冰(bing)箱取(qu)出(chu)的(de)(de)(de)(de)濃(nong)縮(suo)洗滌(di)(di)(di)液(ye)(ye)(ye)(ye)會(hui)有(you)結(jie)晶，這屬于(yu)(yu)(yu)(yu)正常現象，水(shui)浴加熱(re)(re)使結(jie)晶完全溶(rong)解(jie)后(hou)再使用(yong)(yong)。實(shi)(shi)驗(yan)中不(bu)用(yong)(yong)的(de)(de)(de)(de)板(ban)條應立(li)即放回(hui)自封(feng)(feng)袋中，密封(feng)(feng)（低(di)溫干燥）保(bao)(bao)存。濃(nong)度(du)為(wei)0的(de)(de)(de)(de)S0號(hao)標(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)即可視為(wei)陰性(xing)對(dui)照(zhao)(zhao)或者(zhe)空白；按(an)(an)(an)照(zhao)(zhao)說明(ming)(ming)書(shu)操(cao)作時樣(yang)本(ben)已(yi)經稀釋(shi)5倍，＊終結(jie)果乘以5才是(shi)樣(yang)本(ben)實(shi)(shi)際濃(nong)度(du)。嚴(yan)格按(an)(an)(an)照(zhao)(zhao)說明(ming)(ming)書(shu)中標(biao)(biao)(biao)(biao)明(ming)(ming)的(de)(de)(de)(de)時間(jian)、加液(ye)(ye)(ye)(ye)量(liang)(liang)及順序進行(xing)溫育操(cao)作。5. 所(suo)有(you)液(ye)(ye)(ye)(ye)體(ti)組(zu)分(fen)(fen)使用(yong)(yong)前充分(fen)(fen)搖勻(yun)。試(shi)(shi)(shi)劑(ji)(ji)盒(he)組(zu)成(cheng)名稱96孔(kong)(kong)配(pei)(pei)置48孔(kong)(kong)配(pei)(pei)置備注(zhu)(zhu)微孔(kong)(kong)酶(mei)標(biao)(biao)(biao)(biao)板(ban)12孔(kong)(kong)×8條12孔(kong)(kong)×4條無(wu)(wu)標(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管無(wu)(wu)樣(yang)本(ben)稀釋(shi)液(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)檢(jian)測(ce)(ce)(ce)(ce)抗(kang)體(ti)-HRP10mL5mL無(wu)(wu)20×洗滌(di)(di)(di)緩(huan)沖液(ye)(ye)(ye)(ye)25mL15mL按(an)(an)(an)說明(ming)(ming)書(shu)進行(xing)稀釋(shi)底物(wu)(wu)(wu)A6mL3mL無(wu)(wu)底物(wu)(wu)(wu)B6mL3mL無(wu)(wu)終止(zhi)液(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)封(feng)(feng)板(ban)膜2張(zhang)2張(zhang)無(wu)(wu)說明(ming)(ming)書(shu)1份1份無(wu)(wu)自封(feng)(feng)袋1個1個無(wu)(wu)注(zhu)(zhu)：標(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)（S0-S5）濃(nong)度(du)依次為(wei)：0、30、60、120、240、480 ng/mL試(shi)(shi)(shi)劑(ji)(ji)的(de)(de)(de)(de)準(zhun)備 20×洗滌(di)(di)(di)緩(huan)沖液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)稀釋(shi)：蒸餾水(shui)按(an)(an)(an)1：20稀釋(shi)，即1份的(de)(de)(de)(de)20×洗滌(di)(di)(di)緩(huan)沖液(ye)(ye)(ye)(ye)加19份的(de)(de)(de)(de)蒸餾水(shui)。洗板(ban)方法(fa)手工洗板(ban)：甩盡孔(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)體(ti)，每孔(kong)(kong)加滿洗滌(di)(di)(di)液(ye)(ye)(ye)(ye)，靜(jing)置1min后(hou)甩盡孔(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)體(ti)，在(zai)(zai)吸水(shui)紙上(shang)(shang)拍(pai)干，如此洗板(ban)5次。自動洗板(ban)機：每孔(kong)(kong)注(zhu)(zhu)入洗液(ye)(ye)(ye)(ye)350μL，浸泡1min，洗板(ban)5次。操(cao)作步驟 結(jie)果判斷(duan)繪(hui)制標(biao)(biao)(biao)(biao)準(zhun)曲(qu)線：在(zai)(zai)Excel工作表中，以標(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)濃(nong)度(du)作橫坐標(biao)(biao)(biao)(biao)，對(dui)應OD值作縱坐標(biao)(biao)(biao)(biao)，繪(hui)制出(chu)標(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)線性(xing)回(hui)歸曲(qu)線，按(an)(an)(an)曲(qu)線方程計算(suan)各(ge)樣(yang)本(ben)濃(nong)度(du)值。試(shi)(shi)(shi)劑(ji)(ji)盒(he)性(xing)能(neng)(neng)準(zhun)確性(xing)：標(biao)(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)(pin)線性(xing)回(hui)歸與預期濃(nong)度(du)相(xiang)關系(xi)數R值，大于(yu)(yu)(yu)(yu)等(deng)于(yu)(yu)(yu)(yu)0.9900。靈敏度(du)：＊低(di)檢(jian)測(ce)(ce)(ce)(ce)濃(nong)度(du)小于(yu)(yu)(yu)(yu)1.0 pg/mL。特異性(xing)：不(bu)與其它可溶(rong)性(xing)結(jie)構類似(si)物(wu)(wu)(wu)交叉反(fan)應。重復性(xing)：板(ban)內(nei)、板(ban)間(jian)變(bian)異系(xi)數均(jun)(jun)小于(yu)(yu)(yu)(yu)15%。貯藏：2-8℃，避(bi)光防潮保(bao)(bao)存。有(you)效期：6個月(yue)免(mian)責聲明(ming)(ming)試(shi)(shi)(shi)劑(ji)(ji)盒(he)僅供研究使用(yong)(yong)，不(bu)得(de)(de)用(yong)(yong)于(yu)(yu)(yu)(yu)臨床(chuang)實(shi)(shi)驗(yan)或人體(ti)實(shi)(shi)驗(yan)，否則所(suo)產(chan)生的(de)(de)(de)(de)一切后(hou)果，由(you)實(shi)(shi)驗(yan)者(zhe)承(cheng)擔，本(ben)公(gong)司概(gai)不(bu)負責。嚴(yan)格按(an)(an)(an)照(zhao)(zhao)說明(ming)(ming)書(shu)操(cao)作，實(shi)(shi)驗(yan)者(zhe)違反(fan)說明(ming)(ming)書(shu)操(cao)作，后(hou)果由(you)實(shi)(shi)驗(yan)者(zhe)承(cheng)擔。技術支(zhi)持(chi)電話：電子郵件：網(wang)址：fllygs.com生產(chan)廠家：上(shang)(shang)海篤瑪生物(wu)(wu)(wu)科技有(you)限公(gong)司
犬（Canine）喹啉酸（QUIN）ELISA檢(jian)(jian)(jian)測(ce)(ce)試(shi)劑(ji)(ji)(ji)盒本試(shi)劑(ji)(ji)(ji)盒只能用(yong)(yong)于(yu)(yu)(yu)(yu)科學研(yan)究(jiu)，不(bu)得用(yong)(yong)于(yu)(yu)(yu)(yu)醫學診斷(duan)使(shi)(shi)用(yong)(yong)說明(ming)(ming)書(shu)(shu)(shu)產(chan)(chan)品(pin)(pin)(pin)貨號：【DM-Can45212】產(chan)(chan)品(pin)(pin)(pin)規格(ge)：【48T/96T】檢(jian)(jian)(jian)測(ce)(ce)范圍：反應(ying)(ying)(ying)性(xing)：Canine檢(jian)(jian)(jian)測(ce)(ce)范圍：11.37-800 nmol/L靈敏度：＊低(di)檢(jian)(jian)(jian)測(ce)(ce)濃(nong)(nong)(nong)(nong)(nong)度小于(yu)(yu)(yu)(yu)10 nmol/L檢(jian)(jian)(jian)測(ce)(ce)原理試(shi)劑(ji)(ji)(ji)盒采用(yong)(yong)雙抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)一步夾(jia)心(xin)法酶(mei)(mei)聯免疫吸附(fu)試(shi)驗(yan)（ELISA）。往預先(xian)包(bao)被喹啉酸（QUIN）抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)包(bao)被微(wei)孔(kong)中(zhong)(zhong)，依次加(jia)入(ru)標(biao)(biao)(biao)本、標(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)、HRP標(biao)(biao)(biao)記(ji)的(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)，經(jing)(jing)過溫育并(bing)徹(che)底洗(xi)(xi)(xi)(xi)(xi)滌。用(yong)(yong)底物(wu)TMB顯色，TMB在過氧化物(wu)酶(mei)(mei)的(de)(de)(de)催化下轉化成藍(lan)色，并(bing)在酸的(de)(de)(de)作(zuo)用(yong)(yong)下轉化成＊終(zhong)(zhong)的(de)(de)(de)黃色。顏色的(de)(de)(de)深淺和樣(yang)品(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)喹啉酸（QUIN）呈正相關(guan)。用(yong)(yong)酶(mei)(mei)標(biao)(biao)(biao)儀在450nm 波長下測(ce)(ce)定(ding)吸光度（OD 值(zhi)），計(ji)算樣(yang)品(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度。樣(yang)品(pin)(pin)(pin)收(shou)集(ji)(ji)、處(chu)理及保存方(fang)法1. 血(xue)清(qing)：使(shi)(shi)用(yong)(yong)不(bu)含(han)熱(re)原和內(nei)毒素的(de)(de)(de)試(shi)管，操(cao)(cao)(cao)作(zuo)過程中(zhong)(zhong)避免任何細(xi)胞刺激，收(shou)集(ji)(ji)血(xue)液(ye)(ye)后(hou)(hou)，3000轉離(li)心(xin)10分(fen)(fen)(fen)鐘(zhong)將(jiang)血(xue)清(qing)和紅(hong)細(xi)胞迅速小心(xin)地分(fen)(fen)(fen)離(li)。2. 血(xue)漿(jiang)：EDTA、檸檬酸鹽(yan)或肝(gan)素抗(kang)(kang)凝。3000轉離(li)心(xin)30分(fen)(fen)(fen)鐘(zhong)取(qu)上(shang)(shang)清(qing)。3. 細(xi)胞上(shang)(shang)清(qing)液(ye)(ye)：3000轉離(li)心(xin)10分(fen)(fen)(fen)鐘(zhong)去除顆粒和聚合物(wu)。4. 組(zu)織(zhi)勻(yun)漿(jiang)：將(jiang)組(zu)織(zhi)加(jia)入(ru)適量生理鹽(yan)水搗碎。3000轉離(li)心(xin)10分(fen)(fen)(fen)鐘(zhong)取(qu)上(shang)(shang)清(qing)。5. 保存：如(ru)(ru)果(guo)(guo)樣(yang)本收(shou)集(ji)(ji)后(hou)(hou)不(bu)及時(shi)(shi)檢(jian)(jian)(jian)測(ce)(ce)，請按(an)一次用(yong)(yong)量分(fen)(fen)(fen)裝，凍存于(yu)(yu)(yu)(yu)-20℃，避免反復(fu)凍融，在室(shi)溫下解(jie)凍并(bing)確(que)保樣(yang)品(pin)(pin)(pin)均勻(yun)地充分(fen)(fen)(fen)解(jie)凍。自(zi)備(bei)(bei)物(wu)品(pin)(pin)(pin)酶(mei)(mei)標(biao)(biao)(biao)儀（450nm）高精度加(jia)樣(yang)器及槍頭(tou)：0.5-10uL、2-20uL、20-200uL、200-1000uL37℃恒溫箱操(cao)(cao)(cao)作(zuo)注(zhu)(zhu)意(yi)事項試(shi)劑(ji)(ji)(ji)盒保存在2-8℃，使(shi)(shi)用(yong)(yong)前室(shi)溫平衡20分(fen)(fen)(fen)鐘(zhong)。從冰箱取(qu)出的(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)縮洗(xi)(xi)(xi)(xi)(xi)滌液(ye)(ye)會(hui)有(you)結晶，這屬于(yu)(yu)(yu)(yu)正常(chang)現象，水浴加(jia)熱(re)使(shi)(shi)結晶完全溶解(jie)后(hou)(hou)再使(shi)(shi)用(yong)(yong)。實驗(yan)中(zhong)(zhong)不(bu)用(yong)(yong)的(de)(de)(de)板(ban)(ban)條應(ying)(ying)(ying)立即放回自(zi)封袋中(zhong)(zhong)，密(mi)封（低(di)溫干燥）保存。濃(nong)(nong)(nong)(nong)(nong)度為0的(de)(de)(de)S0號標(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)即可視為陰(yin)性(xing)對照(zhao)或者(zhe)(zhe)空白；按(an)照(zhao)說明(ming)(ming)書(shu)(shu)(shu)操(cao)(cao)(cao)作(zuo)時(shi)(shi)樣(yang)本已經(jing)(jing)稀(xi)(xi)釋(shi)(shi)(shi)5倍(bei)，＊終(zhong)(zhong)結果(guo)(guo)乘以(yi)5才是樣(yang)本實際濃(nong)(nong)(nong)(nong)(nong)度。嚴格(ge)按(an)照(zhao)說明(ming)(ming)書(shu)(shu)(shu)中(zhong)(zhong)標(biao)(biao)(biao)明(ming)(ming)的(de)(de)(de)時(shi)(shi)間(jian)、加(jia)液(ye)(ye)量及順序進行溫育操(cao)(cao)(cao)作(zuo)。5. 所有(you)液(ye)(ye)體(ti)(ti)(ti)(ti)(ti)組(zu)分(fen)(fen)(fen)使(shi)(shi)用(yong)(yong)前充分(fen)(fen)(fen)搖勻(yun)。試(shi)劑(ji)(ji)(ji)盒組(zu)成名稱96孔(kong)配置48孔(kong)配置備(bei)(bei)注(zhu)(zhu)微(wei)孔(kong)酶(mei)(mei)標(biao)(biao)(biao)板(ban)(ban)12孔(kong)×8條12孔(kong)×4條無(wu)(wu)標(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)0.3mL*6管0.3mL*6管無(wu)(wu)樣(yang)本稀(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)6mL3mL無(wu)(wu)檢(jian)(jian)(jian)測(ce)(ce)抗(kang)(kang)體(ti)(ti)(ti)(ti)(ti)-HRP10mL5mL無(wu)(wu)20×洗(xi)(xi)(xi)(xi)(xi)滌緩沖(chong)液(ye)(ye)25mL15mL按(an)說明(ming)(ming)書(shu)(shu)(shu)進行稀(xi)(xi)釋(shi)(shi)(shi)底物(wu)A6mL3mL無(wu)(wu)底物(wu)B6mL3mL無(wu)(wu)終(zhong)(zhong)止液(ye)(ye)6mL3mL無(wu)(wu)封板(ban)(ban)膜(mo)2張2張無(wu)(wu)說明(ming)(ming)書(shu)(shu)(shu)1份1份無(wu)(wu)自(zi)封袋1個(ge)1個(ge)無(wu)(wu)注(zhu)(zhu)：標(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)（S0-S5）濃(nong)(nong)(nong)(nong)(nong)度依次為：0、50、100、200、400、800 nmol/L試(shi)劑(ji)(ji)(ji)的(de)(de)(de)準(zhun)備(bei)(bei) 20×洗(xi)(xi)(xi)(xi)(xi)滌緩沖(chong)液(ye)(ye)的(de)(de)(de)稀(xi)(xi)釋(shi)(shi)(shi)：蒸(zheng)餾(liu)水按(an)1：20稀(xi)(xi)釋(shi)(shi)(shi)，即1份的(de)(de)(de)20×洗(xi)(xi)(xi)(xi)(xi)滌緩沖(chong)液(ye)(ye)加(jia)19份的(de)(de)(de)蒸(zheng)餾(liu)水。洗(xi)(xi)(xi)(xi)(xi)板(ban)(ban)方(fang)法手工洗(xi)(xi)(xi)(xi)(xi)板(ban)(ban)：甩(shuai)盡(jin)孔(kong)內(nei)液(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，每孔(kong)加(jia)滿洗(xi)(xi)(xi)(xi)(xi)滌液(ye)(ye)，靜置1min后(hou)(hou)甩(shuai)盡(jin)孔(kong)內(nei)液(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，在吸水紙上(shang)(shang)拍(pai)干，如(ru)(ru)此洗(xi)(xi)(xi)(xi)(xi)板(ban)(ban)5次。自(zi)動(dong)洗(xi)(xi)(xi)(xi)(xi)板(ban)(ban)機：每孔(kong)注(zhu)(zhu)入(ru)洗(xi)(xi)(xi)(xi)(xi)液(ye)(ye)350μL，浸(jin)泡1min，洗(xi)(xi)(xi)(xi)(xi)板(ban)(ban)5次。操(cao)(cao)(cao)作(zuo)步驟 結果(guo)(guo)判斷(duan)繪(hui)制標(biao)(biao)(biao)準(zhun)曲(qu)(qu)線(xian)：在Excel工作(zuo)表中(zhong)(zhong)，以(yi)標(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度作(zuo)橫(heng)坐(zuo)標(biao)(biao)(biao)，對應(ying)(ying)(ying)OD值(zhi)作(zuo)縱坐(zuo)標(biao)(biao)(biao)，繪(hui)制出標(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)線(xian)性(xing)回歸(gui)曲(qu)(qu)線(xian)，按(an)曲(qu)(qu)線(xian)方(fang)程計(ji)算各(ge)樣(yang)本濃(nong)(nong)(nong)(nong)(nong)度值(zhi)。試(shi)劑(ji)(ji)(ji)盒性(xing)能準(zhun)確(que)性(xing)：標(biao)(biao)(biao)準(zhun)品(pin)(pin)(pin)線(xian)性(xing)回歸(gui)與(yu)預期濃(nong)(nong)(nong)(nong)(nong)度相關(guan)系(xi)數R值(zhi)，大(da)于(yu)(yu)(yu)(yu)等于(yu)(yu)(yu)(yu)0.9900。靈敏度：＊低(di)檢(jian)(jian)(jian)測(ce)(ce)濃(nong)(nong)(nong)(nong)(nong)度小于(yu)(yu)(yu)(yu)1.0 pg/mL。特異性(xing)：不(bu)與(yu)其(qi)它可溶性(xing)結構類(lei)似物(wu)交叉(cha)反應(ying)(ying)(ying)。重復(fu)性(xing)：板(ban)(ban)內(nei)、板(ban)(ban)間(jian)變(bian)異系(xi)數均小于(yu)(yu)(yu)(yu)15%。貯藏：2-8℃，避光防(fang)潮保存。有(you)效期：6個(ge)月免責(ze)聲明(ming)(ming)試(shi)劑(ji)(ji)(ji)盒僅供研(yan)究(jiu)使(shi)(shi)用(yong)(yong)，不(bu)得用(yong)(yong)于(yu)(yu)(yu)(yu)臨床實驗(yan)或人體(ti)(ti)(ti)(ti)(ti)實驗(yan)，否則(ze)所產(chan)(chan)生的(de)(de)(de)一切后(hou)(hou)果(guo)(guo)，由實驗(yan)者(zhe)(zhe)承(cheng)(cheng)擔(dan)，本公司(si)概(gai)不(bu)負責(ze)。嚴格(ge)按(an)照(zhao)說明(ming)(ming)書(shu)(shu)(shu)操(cao)(cao)(cao)作(zuo)，實驗(yan)者(zhe)(zhe)違(wei)反說明(ming)(ming)書(shu)(shu)(shu)操(cao)(cao)(cao)作(zuo)，后(hou)(hou)果(guo)(guo)由實驗(yan)者(zhe)(zhe)承(cheng)(cheng)擔(dan)。技術支持電(dian)話：電(dian)子郵(you)件：網(wang)址：fllygs.com生產(chan)(chan)廠(chang)家：上(shang)(shang)海篤瑪(ma)生物(wu)科技有(you)限公司(si)
犬（Canine）犬尿氨(an)酸(suan)(suan)（Kynurenine）ELISA檢測(ce)(ce)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒本(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒只能用(yong)(yong)于科學(xue)研究，不(bu)得用(yong)(yong)于醫學(xue)診斷使(shi)用(yong)(yong)說(shuo)明(ming)(ming)(ming)書(shu)(shu)產(chan)品(pin)貨號：【DM-Can45211】產(chan)品(pin)規格(ge)(ge)：【48T/96T】檢測(ce)(ce)范圍：反(fan)(fan)應(ying)性(xing)(xing)：Canine檢測(ce)(ce)范圍：7.13-480 pg/mL靈敏度(du)(du)(du)： ＊低(di)(di)檢測(ce)(ce)濃(nong)(nong)(nong)度(du)(du)(du)小于1.0 pg/mL檢測(ce)(ce)原理(li)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒采用(yong)(yong)雙抗(kang)體(ti)一(yi)步夾心(xin)(xin)(xin)(xin)法(fa)酶(mei)(mei)聯免(mian)疫吸附(fu)試(shi)(shi)(shi)(shi)驗(yan)（ELISA）。往預先(xian)包(bao)被犬尿氨(an)酸(suan)(suan)（Kynurenine）抗(kang)體(ti)的(de)包(bao)被微(wei)孔(kong)中(zhong)(zhong)，依(yi)次(ci)加(jia)(jia)入標(biao)(biao)(biao)本(ben)(ben)、標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)、HRP標(biao)(biao)(biao)記(ji)的(de)檢測(ce)(ce)抗(kang)體(ti)，經過溫(wen)(wen)育并徹(che)底洗(xi)滌(di)。用(yong)(yong)底物(wu)TMB顯色(se)，TMB在(zai)過氧化(hua)物(wu)酶(mei)(mei)的(de)催化(hua)下轉(zhuan)(zhuan)(zhuan)(zhuan)化(hua)成藍色(se)，并在(zai)酸(suan)(suan)的(de)作(zuo)(zuo)(zuo)用(yong)(yong)下轉(zhuan)(zhuan)(zhuan)(zhuan)化(hua)成＊終(zhong)(zhong)的(de)黃色(se)。顏色(se)的(de)深淺和樣(yang)品(pin)中(zhong)(zhong)的(de)犬尿氨(an)酸(suan)(suan)（Kynurenine）呈正相關。用(yong)(yong)酶(mei)(mei)標(biao)(biao)(biao)儀在(zai)450nm 波長下測(ce)(ce)定吸光度(du)(du)(du)（OD 值），計(ji)算樣(yang)品(pin)濃(nong)(nong)(nong)度(du)(du)(du)。樣(yang)品(pin)收集(ji)(ji)、處理(li)及(ji)保(bao)(bao)(bao)存(cun)(cun)方法(fa)1. 血清(qing)(qing)(qing)：使(shi)用(yong)(yong)不(bu)含熱(re)原和內(nei)(nei)毒素(su)的(de)試(shi)(shi)(shi)(shi)管，操(cao)作(zuo)(zuo)(zuo)過程(cheng)中(zhong)(zhong)避免(mian)任何細胞刺激，收集(ji)(ji)血液(ye)(ye)(ye)(ye)(ye)(ye)后，3000轉(zhuan)(zhuan)(zhuan)(zhuan)離心(xin)(xin)(xin)(xin)10分(fen)(fen)鐘將(jiang)血清(qing)(qing)(qing)和紅細胞迅速小心(xin)(xin)(xin)(xin)地(di)分(fen)(fen)離。2. 血漿：EDTA、檸(ning)檬酸(suan)(suan)鹽或(huo)(huo)肝素(su)抗(kang)凝。3000轉(zhuan)(zhuan)(zhuan)(zhuan)離心(xin)(xin)(xin)(xin)30分(fen)(fen)鐘取上清(qing)(qing)(qing)。3. 細胞上清(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)：3000轉(zhuan)(zhuan)(zhuan)(zhuan)離心(xin)(xin)(xin)(xin)10分(fen)(fen)鐘去除顆粒和聚合物(wu)。4. 組(zu)(zu)(zu)織勻漿：將(jiang)組(zu)(zu)(zu)織加(jia)(jia)入適量(liang)(liang)生(sheng)理(li)鹽水(shui)搗碎。3000轉(zhuan)(zhuan)(zhuan)(zhuan)離心(xin)(xin)(xin)(xin)10分(fen)(fen)鐘取上清(qing)(qing)(qing)。5. 保(bao)(bao)(bao)存(cun)(cun)：如果(guo)樣(yang)本(ben)(ben)收集(ji)(ji)后不(bu)及(ji)時(shi)檢測(ce)(ce)，請按(an)一(yi)次(ci)用(yong)(yong)量(liang)(liang)分(fen)(fen)裝，凍(dong)存(cun)(cun)于-20℃，避免(mian)反(fan)(fan)復(fu)凍(dong)融，在(zai)室(shi)溫(wen)(wen)下解凍(dong)并確保(bao)(bao)(bao)樣(yang)品(pin)均勻地(di)充分(fen)(fen)解凍(dong)。自備物(wu)品(pin)酶(mei)(mei)標(biao)(biao)(biao)儀（450nm）高(gao)精度(du)(du)(du)加(jia)(jia)樣(yang)器(qi)及(ji)槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL37℃恒溫(wen)(wen)箱操(cao)作(zuo)(zuo)(zuo)注(zhu)意事項試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒保(bao)(bao)(bao)存(cun)(cun)在(zai)2-8℃，使(shi)用(yong)(yong)前(qian)室(shi)溫(wen)(wen)平衡(heng)20分(fen)(fen)鐘。從冰箱取出的(de)濃(nong)(nong)(nong)縮洗(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)(ye)會有結(jie)(jie)晶，這屬于正常現象，水(shui)浴(yu)加(jia)(jia)熱(re)使(shi)結(jie)(jie)晶完全溶解后再使(shi)用(yong)(yong)。實(shi)驗(yan)中(zhong)(zhong)不(bu)用(yong)(yong)的(de)板(ban)條(tiao)應(ying)立即放回自封袋中(zhong)(zhong)，密封（低(di)(di)溫(wen)(wen)干(gan)燥(zao)）保(bao)(bao)(bao)存(cun)(cun)。濃(nong)(nong)(nong)度(du)(du)(du)為0的(de)S0號標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)即可視為陰(yin)性(xing)(xing)對(dui)照或(huo)(huo)者空白(bai)；按(an)照說(shuo)明(ming)(ming)(ming)書(shu)(shu)操(cao)作(zuo)(zuo)(zuo)時(shi)樣(yang)本(ben)(ben)已經稀釋(shi)(shi)5倍，＊終(zhong)(zhong)結(jie)(jie)果(guo)乘(cheng)以5才是樣(yang)本(ben)(ben)實(shi)際濃(nong)(nong)(nong)度(du)(du)(du)。嚴格(ge)(ge)按(an)照說(shuo)明(ming)(ming)(ming)書(shu)(shu)中(zhong)(zhong)標(biao)(biao)(biao)明(ming)(ming)(ming)的(de)時(shi)間、加(jia)(jia)液(ye)(ye)(ye)(ye)(ye)(ye)量(liang)(liang)及(ji)順序進行溫(wen)(wen)育操(cao)作(zuo)(zuo)(zuo)。5. 所(suo)有液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)組(zu)(zu)(zu)分(fen)(fen)使(shi)用(yong)(yong)前(qian)充分(fen)(fen)搖勻。試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒組(zu)(zu)(zu)成名稱96孔(kong)配置(zhi)48孔(kong)配置(zhi)備注(zhu)微(wei)孔(kong)酶(mei)(mei)標(biao)(biao)(biao)板(ban)12孔(kong)×8條(tiao)12孔(kong)×4條(tiao)無(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)0.3mL*6管0.3mL*6管無(wu)(wu)(wu)(wu)(wu)(wu)樣(yang)本(ben)(ben)稀釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)(wu)(wu)(wu)(wu)檢測(ce)(ce)抗(kang)體(ti)-HRP10mL5mL無(wu)(wu)(wu)(wu)(wu)(wu)20×洗(xi)滌(di)緩(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)25mL15mL按(an)說(shuo)明(ming)(ming)(ming)書(shu)(shu)進行稀釋(shi)(shi)底物(wu)A6mL3mL無(wu)(wu)(wu)(wu)(wu)(wu)底物(wu)B6mL3mL無(wu)(wu)(wu)(wu)(wu)(wu)終(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)(wu)(wu)(wu)(wu)封板(ban)膜2張2張無(wu)(wu)(wu)(wu)(wu)(wu)說(shuo)明(ming)(ming)(ming)書(shu)(shu)1份1份無(wu)(wu)(wu)(wu)(wu)(wu)自封袋1個1個無(wu)(wu)(wu)(wu)(wu)(wu)注(zhu)：標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)（S0-S5）濃(nong)(nong)(nong)度(du)(du)(du)依(yi)次(ci)為：0、30、60、120、240、480 pg/mL試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)的(de)準(zhun)(zhun)(zhun)備 20×洗(xi)滌(di)緩(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)稀釋(shi)(shi)：蒸(zheng)餾水(shui)按(an)1：20稀釋(shi)(shi)，即1份的(de)20×洗(xi)滌(di)緩(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)加(jia)(jia)19份的(de)蒸(zheng)餾水(shui)。洗(xi)板(ban)方法(fa)手工(gong)(gong)洗(xi)板(ban)：甩(shuai)盡(jin)(jin)孔(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，每(mei)孔(kong)加(jia)(jia)滿洗(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)(ye)，靜置(zhi)1min后甩(shuai)盡(jin)(jin)孔(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，在(zai)吸水(shui)紙上拍干(gan)，如此洗(xi)板(ban)5次(ci)。自動洗(xi)板(ban)機：每(mei)孔(kong)注(zhu)入洗(xi)液(ye)(ye)(ye)(ye)(ye)(ye)350μL，浸泡(pao)1min，洗(xi)板(ban)5次(ci)。操(cao)作(zuo)(zuo)(zuo)步驟(zou) 結(jie)(jie)果(guo)判(pan)斷繪制(zhi)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲線(xian)：在(zai)Excel工(gong)(gong)作(zuo)(zuo)(zuo)表中(zhong)(zhong)，以標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)濃(nong)(nong)(nong)度(du)(du)(du)作(zuo)(zuo)(zuo)橫(heng)坐(zuo)標(biao)(biao)(biao)，對(dui)應(ying)OD值作(zuo)(zuo)(zuo)縱坐(zuo)標(biao)(biao)(biao)，繪制(zhi)出標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)線(xian)性(xing)(xing)回歸曲線(xian)，按(an)曲線(xian)方程(cheng)計(ji)算各樣(yang)本(ben)(ben)濃(nong)(nong)(nong)度(du)(du)(du)值。試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒性(xing)(xing)能準(zhun)(zhun)(zhun)確性(xing)(xing)：標(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)線(xian)性(xing)(xing)回歸與預期(qi)濃(nong)(nong)(nong)度(du)(du)(du)相關系數(shu)R值，大于等于0.9900。靈敏度(du)(du)(du)：＊低(di)(di)檢測(ce)(ce)濃(nong)(nong)(nong)度(du)(du)(du)小于1.0 pg/mL。特異性(xing)(xing)：不(bu)與其它可溶性(xing)(xing)結(jie)(jie)構類似物(wu)交(jiao)叉反(fan)(fan)應(ying)。重復(fu)性(xing)(xing)：板(ban)內(nei)(nei)、板(ban)間變異系數(shu)均小于15%。貯(zhu)藏(zang)：2-8℃，避光防(fang)潮保(bao)(bao)(bao)存(cun)(cun)。有效(xiao)期(qi)：6個月免(mian)責(ze)(ze)聲(sheng)明(ming)(ming)(ming)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒僅供研究使(shi)用(yong)(yong)，不(bu)得用(yong)(yong)于臨床實(shi)驗(yan)或(huo)(huo)人體(ti)實(shi)驗(yan)，否則所(suo)產(chan)生(sheng)的(de)一(yi)切后果(guo)，由實(shi)驗(yan)者承擔，本(ben)(ben)公司概不(bu)負責(ze)(ze)。嚴格(ge)(ge)按(an)照說(shuo)明(ming)(ming)(ming)書(shu)(shu)操(cao)作(zuo)(zuo)(zuo)，實(shi)驗(yan)者違反(fan)(fan)說(shuo)明(ming)(ming)(ming)書(shu)(shu)操(cao)作(zuo)(zuo)(zuo)，后果(guo)由實(shi)驗(yan)者承擔。技術支持電話：電子郵件：網(wang)址：fllygs.com生(sheng)產(chan)廠(chang)家：上海(hai)篤瑪生(sheng)物(wu)科技有限(xian)公司
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