牛(niu)類(lei)(lei)琥(hu)珀(po)酸半醛(quan)脫(tuo)氫(qing)酶(mei)(mei)(SSADH)ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)文(wen)(wen)名稱牛(niu)類(lei)(lei)琥(hu)珀(po)酸半醛(quan)脫(tuo)氫(qing)酶(mei)(mei)(SSADH)ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)規格96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)本類(lei)(lei)型(xing)血(xue)清、血(xue)漿、細胞(bao)上清液(ye)(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)(ye)、體液(ye)(ye)(ye)(ye)(ye)、灌洗液(ye)(ye)(ye)(ye)(ye)等使用(yong)(yong)(yong)(yong)(yong)范圍(wei)科(ke)研(yan)實(shi)驗(yan)(yan)，不(bu)(bu)得用(yong)(yong)(yong)(yong)(yong)于(yu)臨床診(zhen)斷反應(ying)(ying)時(shi)(shi)(shi)(shi)間1-5h試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)保(bao)(bao)存(cun)2-8℃有(you)效期6個月貨期3-5天【操(cao)作(zuo)(zuo)(zuo)步驟】1. 標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)與加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)(zai)(zai)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)包被(bei)板(ban)(ban)上設(she)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)一(yi)、第(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，然(ran)后(hou)(hou)(hou)(hou)(hou)(hou)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)一(yi)、第(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)；然(ran)后(hou)(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)一(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)100μl分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)(he)第(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)；然(ran)后(hou)(hou)(hou)(hou)(hou)(hou)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)(he)第(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)先(xian)各(ge)(ge)取(qu)50μl棄(qi)掉(diao)，再(zai)(zai)各(ge)(ge)取(qu)50μl分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50ul，混(hun)勻(yun)；混(hun)勻(yun)后(hou)(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)50μl分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)后(hou)(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)取(qu)50μl加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)九、第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)后(hou)(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)50μl棄(qi)掉(diao)。（稀(xi)(xi)(xi)(xi)釋(shi)(shi)后(hou)(hou)(hou)(hou)(hou)(hou)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)量(liang)都為50μl，濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)為3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)設(she)空(kong)(kong)白(bai)(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)(kong)白(bai)(bai)(bai)對(dui)照孔(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)及(ji)(ji)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，其余各(ge)(ge)步操(cao)作(zuo)(zuo)(zuo)相(xiang)同(tong)(tong)）、待測(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)(zai)(zai)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)包被(bei)板(ban)(ban)上待測(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)40μl，然(ran)后(hou)(hou)(hou)(hou)(hou)(hou)再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)待測(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)＊終(zhong)稀(xi)(xi)(xi)(xi)釋(shi)(shi)度(du)(du)(du)(du)為5倍(bei)(bei)(bei)(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)將樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)底部，盡量(liang)不(bu)(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)壁，輕輕晃動混(hun)勻(yun)。3. 溫育(yu)：用(yong)(yong)(yong)(yong)(yong)封板(ban)(ban)膜封板(ban)(ban)后(hou)(hou)(hou)(hou)(hou)(hou)置(zhi)37℃溫育(yu)30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。4. 配液(ye)(ye)(ye)(ye)(ye)：將30（48T的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)20倍(bei)(bei)(bei)(bei)(bei)）倍(bei)(bei)(bei)(bei)(bei)濃(nong)(nong)(nong)(nong)縮洗滌液(ye)(ye)(ye)(ye)(ye)用(yong)(yong)(yong)(yong)(yong)蒸餾水30（48T的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)20倍(bei)(bei)(bei)(bei)(bei)）倍(bei)(bei)(bei)(bei)(bei)稀(xi)(xi)(xi)(xi)釋(shi)(shi)后(hou)(hou)(hou)(hou)(hou)(hou)備用(yong)(yong)(yong)(yong)(yong)。5. 洗滌：小(xiao)心(xin)揭掉(diao)封板(ban)(ban)膜，棄(qi)去(qu)液(ye)(ye)(ye)(ye)(ye)體，甩干(gan)，每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)滿(man)洗滌液(ye)(ye)(ye)(ye)(ye)，靜置(zhi)30秒后(hou)(hou)(hou)(hou)(hou)(hou)棄(qi)去(qu)，如此(ci)(ci)重(zhong)(zhong)復5次(ci)，拍干(gan)。6. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)50μl，空(kong)(kong)白(bai)(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)除外。7. 溫育(yu)：操(cao)作(zuo)(zuo)(zuo)同(tong)(tong)3。8. 洗滌：操(cao)作(zuo)(zuo)(zuo)同(tong)(tong)5。9. 顯色(se)(se)(se)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入顯色(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)A50μl，再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入顯色(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)B50μl，輕輕震蕩混(hun)勻(yun)，37℃避光顯色(se)(se)(se)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong). 10. 終(zhong)止(zhi)(zhi)(zhi)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止(zhi)(zhi)(zhi)反應(ying)(ying)（此(ci)(ci)時(shi)(shi)(shi)(shi)藍色(se)(se)(se)立轉黃色(se)(se)(se)）。11. 測(ce)定(ding)(ding)(ding)(ding)：以(yi)(yi)(yi)空(kong)(kong)白(bai)(bai)(bai)空(kong)(kong)調零(ling)，450nm波長依(yi)序測(ce)量(liang)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)吸光度(du)(du)(du)(du)（OD值(zhi)(zhi)）。 測(ce)定(ding)(ding)(ding)(ding)應(ying)(ying)在(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)內(nei)(nei)進(jin)(jin)行(xing)(xing)。牛(niu)類(lei)(lei)琥(hu)珀(po)酸半醛(quan)脫(tuo)氫(qing)酶(mei)(mei)(SSADH)ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)【注意(yi)事(shi)項】1． 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)從(cong)冷藏環(huan)境(jing)中(zhong)(zhong)(zhong)(zhong)(zhong)取(qu)出(chu)應(ying)(ying)在(zai)(zai)(zai)(zai)室(shi)溫平衡15-30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)(hou)(hou)(hou)(hou)方可(ke)使用(yong)(yong)(yong)(yong)(yong)，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)包被(bei)板(ban)(ban)開(kai)封后(hou)(hou)(hou)(hou)(hou)(hou)如未(wei)用(yong)(yong)(yong)(yong)(yong)完(wan)，板(ban)(ban)條應(ying)(ying)裝入密封袋中(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)存(cun)。2． 濃(nong)(nong)(nong)(nong)洗滌液(ye)(ye)(ye)(ye)(ye)可(ke)能會有(you)結晶析出(chu)，稀(xi)(xi)(xi)(xi)釋(shi)(shi)時(shi)(shi)(shi)(shi)可(ke)在(zai)(zai)(zai)(zai)水浴(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)溫助(zhu)溶(rong)，洗滌時(shi)(shi)(shi)(shi)不(bu)(bu)影響結果(guo)(guo)。3． 各(ge)(ge)步加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)均(jun)(jun)應(ying)(ying)使用(yong)(yong)(yong)(yong)(yong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)器，并(bing)經(jing)(jing)常校對(dui)其準(zhun)(zhun)(zhun)確性(xing)(xing)，以(yi)(yi)(yi)避免(mian)試(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)誤差(cha)(cha)。一(yi)次(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)間＊好控(kong)制在(zai)(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)，如標(biao)(biao)(biao)(biao)(biao)本數量(liang)多(duo)，推薦(jian)使用(yong)(yong)(yong)(yong)(yong)排(pai)槍(qiang)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)。4． 請每(mei)次(ci)測(ce)定(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)(tong)時(shi)(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線，＊好做(zuo)復孔(kong)(kong)(kong)(kong)(kong)(kong)。如標(biao)(biao)(biao)(biao)(biao)本中(zhong)(zhong)(zhong)(zhong)(zhong)待測(ce)物(wu)(wu)(wu)質(zhi)(zhi)含量(liang)過(guo)高(gao)（樣(yang)(yang)(yang)(yang)(yang)(yang)本OD值(zhi)(zhi)大于(yu)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)一(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)），請先(xian)用(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)一(yi)定(ding)(ding)(ding)(ding)倍(bei)(bei)(bei)(bei)(bei)數（n倍(bei)(bei)(bei)(bei)(bei)）后(hou)(hou)(hou)(hou)(hou)(hou)再(zai)(zai)測(ce)定(ding)(ding)(ding)(ding)，計(ji)(ji)算(suan)(suan)時(shi)(shi)(shi)(shi)請＊后(hou)(hou)(hou)(hou)(hou)(hou)乘(cheng)以(yi)(yi)(yi)總稀(xi)(xi)(xi)(xi)釋(shi)(shi)倍(bei)(bei)(bei)(bei)(bei)數（×n×5）。5． 封板(ban)(ban)膜只(zhi)限(xian)一(yi)次(ci)性(xing)(xing)使用(yong)(yong)(yong)(yong)(yong)，以(yi)(yi)(yi)避免(mian)交(jiao)(jiao)叉(cha)(cha)(cha)污染。6． 底物(wu)(wu)(wu)請避光保(bao)(bao)存(cun)。7． 嚴格按照說明(ming)(ming)書(shu)(shu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)操(cao)作(zuo)(zuo)(zuo)進(jin)(jin)行(xing)(xing)，試(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)結果(guo)(guo)判(pan)定(ding)(ding)(ding)(ding)必須以(yi)(yi)(yi)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)儀讀數為準(zhun)(zhun)(zhun).8． 所(suo)有(you)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)，洗滌液(ye)(ye)(ye)(ye)(ye)和(he)(he)(he)各(ge)(ge)種廢棄(qi)物(wu)(wu)(wu)都應(ying)(ying)按傳染物(wu)(wu)(wu)處理。9． 本試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)不(bu)(bu)同(tong)(tong)批(pi)(pi)號組(zu)分(fen)(fen)(fen)(fen)不(bu)(bu)得混(hun)用(yong)(yong)(yong)(yong)(yong)。10. 如與英(ying)文(wen)(wen)說明(ming)(ming)書(shu)(shu)有(you)異，以(yi)(yi)(yi)英(ying)文(wen)(wen)說明(ming)(ming)書(shu)(shu)為準(zhun)(zhun)(zhun)。【計(ji)(ji)算(suan)(suan)】 以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)為橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)為縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)紙上繪(hui)出(chu)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線，根據樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD 值(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線查出(chu)相(xiang)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)；再(zai)(zai)乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi) 倍(bei)(bei)(bei)(bei)(bei)數；或用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)與OD值(zhi)(zhi)計(ji)(ji)算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線回歸(gui)方程式，將樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi) 代入方程式，計(ji)(ji)算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)，再(zai)(zai)乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi) 倍(bei)(bei)(bei)(bei)(bei)數，即(ji)為樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)。 【試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)組(zu)成】試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)組(zu)成48孔(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)保(bao)(bao)存(cun)說明(ming)(ming)書(shu)(shu)1份(fen)1份(fen)密封袋1個1個封板(ban)(ban)膜12孔(kong)(kong)(kong)(kong)(kong)(kong)×4條2片（96）2-8℃保(bao)(bao)存(cun)微(wei)(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)板(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)×8條2-8℃保(bao)(bao)存(cun)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管(guan)0.3mL*6管(guan)2-8℃保(bao)(bao)存(cun)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)存(cun)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)存(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)存(cun)顯色(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)存(cun)顯色(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)存(cun)終(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)存(cun)注意(yi)：使用(yong)(yong)(yong)(yong)(yong)前請檢查試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)簽和(he)(he)(he)數量(liang)與表格是否一(yi)致(zhi)。【精密度(du)(du)(du)(du)】精密度(du)(du)(du)(du)用(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)測(ce)定(ding)(ding)(ding)(ding)值(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)變異系數CV 表示。CV（%） = SD/mean×100批(pi)(pi)內(nei)(nei)差(cha)(cha)：取(qu)同(tong)(tong)批(pi)(pi)次(ci)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)對(dui)低、中(zhong)(zhong)(zhong)(zhong)(zhong)、高(gao)值(zhi)(zhi)定(ding)(ding)(ding)(ding)值(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)本進(jin)(jin)行(xing)(xing)定(ding)(ding)(ding)(ding)量(liang)檢測(ce)，每(mei)份(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)本連續測(ce)定(ding)(ding)(ding)(ding)20 次(ci)，分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)計(ji)(ji)算(suan)(suan)不(bu)(bu)同(tong)(tong)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)本的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)平均(jun)(jun)值(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)。批(pi)(pi)內(nei)(nei)差(cha)(cha)： CV<9%批(pi)(pi)間差(cha)(cha)：選取(qu)3 個不(bu)(bu)同(tong)(tong)批(pi)(pi)次(ci)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)對(dui)低、中(zhong)(zhong)(zhong)(zhong)(zhong)、高(gao)值(zhi)(zhi)定(ding)(ding)(ding)(ding)值(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)本進(jin)(jin)行(xing)(xing)定(ding)(ding)(ding)(ding)量(liang)測(ce)定(ding)(ding)(ding)(ding)，每(mei)個樣(yang)(yang)(yang)(yang)(yang)(yang)本使用(yong)(yong)(yong)(yong)(yong)同(tong)(tong)一(yi)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)重(zhong)(zhong)復測(ce)定(ding)(ding)(ding)(ding)10 次(ci)，分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)計(ji)(ji)算(suan)(suan)不(bu)(bu)同(tong)(tong)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)本的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)平均(jun)(jun)值(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)。批(pi)(pi)間差(cha)(cha)： CV<11% 【特異性(xing)(xing)】本試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)識別(bie)(bie)(bie)天然(ran)和(he)(he)(he)重(zhong)(zhong)組(zu)微(wei)(wei)生(sheng)物(wu)(wu)(wu)硝酸鹽還原酶(mei)(mei)（Nar），經(jing)(jing)檢測(ce)與其它相(xiang)似物(wu)(wu)(wu)質(zhi)(zhi)無(wu)明(ming)(ming)顯交(jiao)(jiao)叉(cha)(cha)(cha)反應(ying)(ying)。由于(yu)受到(dao)技術及(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)本來(lai)源的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)制，不(bu)(bu)可(ke)能完(wan)成所(suo)有(you)相(xiang)關(guan)或相(xiang)似物(wu)(wu)(wu)質(zhi)(zhi)交(jiao)(jiao)叉(cha)(cha)(cha)反應(ying)(ying)檢測(ce)，因此(ci)(ci)本試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)有(you)可(ke)能與未(wei)經(jing)(jing)檢測(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)其它物(wu)(wu)(wu)質(zhi)(zhi)有(you)交(jiao)(jiao)叉(cha)(cha)(cha)反應(ying)(ying)。 【穩定(ding)(ding)(ding)(ding)性(xing)(xing)】試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)在(zai)(zai)(zai)(zai)有(you)效期內(nei)(nei)務必按推薦(jian)溫度(du)(du)(du)(du)保(bao)(bao)存(cun)，活性(xing)(xing)降低率小(xiao)于(yu)5%。為減小(xiao)外部因素(su)對(dui)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)破壞前后(hou)(hou)(hou)(hou)(hou)(hou)檢測(ce)值(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)影響，實(shi)驗(yan)(yan)室(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)環(huan)境(jing)條件(jian)需(xu)盡量(liang)保(bao)(bao)持一(yi)致(zhi)，尤其是實(shi)驗(yan)(yan)室(shi)內(nei)(nei)溫度(du)(du)(du)(du)、濕度(du)(du)(du)(du)及(ji)(ji)溫育(yu)條件(jian)。其次(ci)由同(tong)(tong)一(yi)實(shi)驗(yan)(yan)員來(lai)進(jin)(jin)行(xing)(xing)操(cao)作(zuo)(zuo)(zuo)可(ke)減少(shao)人(ren)為誤差(cha)(cha)。
牛(niu)白(bai)介素(su)(su)21(IL-21)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)文(wen)(wen)名稱牛(niu)白(bai)介素(su)(su)21(IL-21)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)規格96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)類型血(xue)清、血(xue)漿、細胞上(shang)清液(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)、體液(ye)(ye)(ye)(ye)、灌洗(xi)(xi)(xi)液(ye)(ye)(ye)(ye)等使用(yong)(yong)(yong)(yong)范圍科研實(shi)(shi)驗，不(bu)(bu)得(de)用(yong)(yong)(yong)(yong)于臨床診斷反(fan)應時(shi)(shi)(shi)間1-5h試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)2-8℃有(you)(you)(you)效期6個(ge)月貨(huo)期3-5天(tian)【操(cao)作(zuo)(zuo)步驟(zou)】1. 標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)與(yu)(yu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)(zai)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)(ban)上(shang)設標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)(di)二(er)(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，然(ran)(ran)后在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)(di)二(er)(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)；然(ran)(ran)后從(cong)第(di)(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)二(er)(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)(ge)(ge)取(qu)100μl分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)(dao)(dao)第(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)；然(ran)(ran)后在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)先(xian)各(ge)(ge)(ge)取(qu)50μl棄(qi)掉，再(zai)(zai)(zai)(zai)各(ge)(ge)(ge)取(qu)50μl分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)(dao)(dao)第(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)50ul，混(hun)(hun)(hun)勻(yun)；混(hun)(hun)(hun)勻(yun)后從(cong)第(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)(ge)(ge)取(qu)50μl分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)(dao)(dao)第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)后從(cong)第(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)取(qu)50μl加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)(dao)(dao)第(di)(di)(di)(di)(di)(di)九(jiu)、第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)后從(cong)第(di)(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)(ge)(ge)取(qu)50μl棄(qi)掉。（稀(xi)(xi)(xi)(xi)釋(shi)(shi)后各(ge)(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)都(dou)為(wei)(wei)(wei)50μl，濃(nong)(nong)度(du)(du)(du)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)為(wei)(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)設空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)白(bai)對照孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)及(ji)(ji)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，其(qi)余各(ge)(ge)(ge)步操(cao)作(zuo)(zuo)相(xiang)(xiang)同(tong)(tong)(tong)）、待(dai)(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)(zai)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)(ban)上(shang)待(dai)(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)40μl，然(ran)(ran)后再(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)待(dai)(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)＊終(zhong)稀(xi)(xi)(xi)(xi)釋(shi)(shi)度(du)(du)(du)為(wei)(wei)(wei)5倍）。加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)于酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部，盡(jin)量(liang)(liang)不(bu)(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕輕晃動(dong)混(hun)(hun)(hun)勻(yun)。3. 溫(wen)育：用(yong)(yong)(yong)(yong)封(feng)(feng)(feng)板(ban)(ban)(ban)膜(mo)封(feng)(feng)(feng)板(ban)(ban)(ban)后置(zhi)37℃溫(wen)育30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。4. 配(pei)液(ye)(ye)(ye)(ye)：將(jiang)30（48T的(de)(de)(de)20倍）倍濃(nong)(nong)縮洗(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)用(yong)(yong)(yong)(yong)蒸(zheng)餾(liu)水30（48T的(de)(de)(de)20倍）倍稀(xi)(xi)(xi)(xi)釋(shi)(shi)后備(bei)用(yong)(yong)(yong)(yong)。5. 洗(xi)(xi)(xi)滌(di)(di)：小(xiao)(xiao)心揭掉封(feng)(feng)(feng)板(ban)(ban)(ban)膜(mo)，棄(qi)去液(ye)(ye)(ye)(ye)體，甩干，每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)，靜(jing)置(zhi)30秒后棄(qi)去，如(ru)(ru)此(ci)(ci)重復(fu)5次(ci)，拍干。6. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)(mei)：每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)50μl，空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外。7. 溫(wen)育：操(cao)作(zuo)(zuo)同(tong)(tong)(tong)3。8. 洗(xi)(xi)(xi)滌(di)(di)：操(cao)作(zuo)(zuo)同(tong)(tong)(tong)5。9. 顯(xian)(xian)(xian)色(se)：每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)顯(xian)(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)A50μl，再(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)顯(xian)(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)B50μl，輕輕震蕩混(hun)(hun)(hun)勻(yun)，37℃避(bi)光顯(xian)(xian)(xian)色(se)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong). 10. 終(zhong)止(zhi)：每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)液(ye)(ye)(ye)(ye)50μl，終(zhong)止(zhi)反(fan)應（此(ci)(ci)時(shi)(shi)(shi)藍色(se)立轉黃色(se)）。11. 測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)：以(yi)空(kong)白(bai)空(kong)調零，450nm波長(chang)依序測(ce)(ce)(ce)量(liang)(liang)各(ge)(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)吸光度(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)應在(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)液(ye)(ye)(ye)(ye)后15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)以(yi)內進(jin)(jin)行。牛(niu)白(bai)介素(su)(su)21(IL-21)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)【注(zhu)意(yi)事項(xiang)】1． 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)從(cong)冷藏環境中(zhong)(zhong)(zhong)(zhong)取(qu)出應在(zai)(zai)(zai)室(shi)溫(wen)平衡(heng)15-30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)后方(fang)可使用(yong)(yong)(yong)(yong)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)(ban)開(kai)封(feng)(feng)(feng)后如(ru)(ru)未(wei)用(yong)(yong)(yong)(yong)完，板(ban)(ban)(ban)條(tiao)應裝入(ru)密封(feng)(feng)(feng)袋中(zhong)(zhong)(zhong)(zhong)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。2． 濃(nong)(nong)洗(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)可能(neng)會有(you)(you)(you)結晶析出，稀(xi)(xi)(xi)(xi)釋(shi)(shi)時(shi)(shi)(shi)可在(zai)(zai)(zai)水浴中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)溫(wen)助(zhu)溶，洗(xi)(xi)(xi)滌(di)(di)時(shi)(shi)(shi)不(bu)(bu)影響(xiang)結果。3． 各(ge)(ge)(ge)步加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)均(jun)(jun)應使用(yong)(yong)(yong)(yong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)器，并經(jing)常校對其(qi)準(zhun)(zhun)(zhun)(zhun)確性(xing)，以(yi)避(bi)免(mian)試(shi)(shi)(shi)(shi)驗誤(wu)(wu)差(cha)(cha)。一(yi)(yi)次(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)間＊好(hao)控制在(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)數(shu)(shu)(shu)量(liang)(liang)多，推薦(jian)使用(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4． 請每(mei)(mei)(mei)(mei)次(ci)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)同(tong)(tong)(tong)時(shi)(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲線(xian)，＊好(hao)做復(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)中(zhong)(zhong)(zhong)(zhong)待(dai)(dai)測(ce)(ce)(ce)物(wu)(wu)(wu)質含量(liang)(liang)過(guo)高(gao)（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)大于標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)），請先(xian)用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)一(yi)(yi)定(ding)(ding)(ding)(ding)(ding)(ding)倍數(shu)(shu)(shu)（n倍）后再(zai)(zai)(zai)(zai)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)，計(ji)算(suan)時(shi)(shi)(shi)請＊后乘以(yi)總稀(xi)(xi)(xi)(xi)釋(shi)(shi)倍數(shu)(shu)(shu)（×n×5）。5． 封(feng)(feng)(feng)板(ban)(ban)(ban)膜(mo)只(zhi)限一(yi)(yi)次(ci)性(xing)使用(yong)(yong)(yong)(yong)，以(yi)避(bi)免(mian)交(jiao)叉(cha)(cha)(cha)污染。6． 底物(wu)(wu)(wu)請避(bi)光保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。7． 嚴格按(an)照說(shuo)明書(shu)的(de)(de)(de)操(cao)作(zuo)(zuo)進(jin)(jin)行，試(shi)(shi)(shi)(shi)驗結果判定(ding)(ding)(ding)(ding)(ding)(ding)必須以(yi)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)儀讀數(shu)(shu)(shu)為(wei)(wei)(wei)準(zhun)(zhun)(zhun)(zhun).8． 所有(you)(you)(you)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)，洗(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)和各(ge)(ge)(ge)種廢棄(qi)物(wu)(wu)(wu)都(dou)應按(an)傳染物(wu)(wu)(wu)處理(li)。9． 本(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)不(bu)(bu)同(tong)(tong)(tong)批(pi)號組(zu)分(fen)(fen)(fen)(fen)(fen)不(bu)(bu)得(de)混(hun)(hun)(hun)用(yong)(yong)(yong)(yong)。10. 如(ru)(ru)與(yu)(yu)英(ying)文(wen)(wen)說(shuo)明書(shu)有(you)(you)(you)異(yi)，以(yi)英(ying)文(wen)(wen)說(shuo)明書(shu)為(wei)(wei)(wei)準(zhun)(zhun)(zhun)(zhun)。【計(ji)算(suan)】 以(yi)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)(de)(de)濃(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)(wei)(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)繪出標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲線(xian)，根據樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)OD 值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲線(xian)查(cha)出相(xiang)(xiang)應的(de)(de)(de)濃(nong)(nong)度(du)(du)(du)；再(zai)(zai)(zai)(zai)乘以(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi) 倍數(shu)(shu)(shu)；或用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)(de)(de)濃(nong)(nong)度(du)(du)(du)與(yu)(yu)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)計(ji)算(suan)出標(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)曲線(xian)的(de)(de)(de)直線(xian)回(hui)歸方(fang)程式，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi) 代(dai)入(ru)方(fang)程式，計(ji)算(suan)出樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)，再(zai)(zai)(zai)(zai)乘以(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi) 倍數(shu)(shu)(shu)，即為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)實(shi)(shi)際(ji)濃(nong)(nong)度(du)(du)(du)。 【試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)組(zu)成】試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)組(zu)成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)說(shuo)明書(shu)1份(fen)1份(fen)密封(feng)(feng)(feng)袋1個(ge)1個(ge)封(feng)(feng)(feng)板(ban)(ban)(ban)膜(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)2片(pian)（96）2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)顯(xian)(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)顯(xian)(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)終(zhong)止(zhi)液(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)注(zhu)意(yi)：使用(yong)(yong)(yong)(yong)前請檢(jian)查(cha)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)簽和數(shu)(shu)(shu)量(liang)(liang)與(yu)(yu)表格是(shi)否一(yi)(yi)致(zhi)。【精密度(du)(du)(du)】精密度(du)(du)(du)用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)變異(yi)系數(shu)(shu)(shu)CV 表示。CV（%） = SD/mean×100批(pi)內差(cha)(cha)：取(qu)同(tong)(tong)(tong)批(pi)次(ci)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)對低、中(zhong)(zhong)(zhong)(zhong)、高(gao)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)進(jin)(jin)行定(ding)(ding)(ding)(ding)(ding)(ding)量(liang)(liang)檢(jian)測(ce)(ce)(ce)，每(mei)(mei)(mei)(mei)份(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)連續(xu)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)20 次(ci)，分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)計(ji)算(suan)不(bu)(bu)同(tong)(tong)(tong)濃(nong)(nong)度(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)(de)平均(jun)(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。批(pi)內差(cha)(cha)： CV<9%批(pi)間差(cha)(cha)：選取(qu)3 個(ge)不(bu)(bu)同(tong)(tong)(tong)批(pi)次(ci)的(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)對低、中(zhong)(zhong)(zhong)(zhong)、高(gao)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)進(jin)(jin)行定(ding)(ding)(ding)(ding)(ding)(ding)量(liang)(liang)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)，每(mei)(mei)(mei)(mei)個(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)使用(yong)(yong)(yong)(yong)同(tong)(tong)(tong)一(yi)(yi)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)重復(fu)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)10 次(ci)，分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)計(ji)算(suan)不(bu)(bu)同(tong)(tong)(tong)濃(nong)(nong)度(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)(de)平均(jun)(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。批(pi)間差(cha)(cha)： CV<11% 【特異(yi)性(xing)】本(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)識別(bie)(bie)(bie)(bie)(bie)天(tian)然(ran)(ran)和重組(zu)微(wei)生物(wu)(wu)(wu)硝(xiao)酸(suan)鹽還原(yuan)酶(mei)(mei)(mei)（Nar），經(jing)檢(jian)測(ce)(ce)(ce)與(yu)(yu)其(qi)它(ta)相(xiang)(xiang)似(si)物(wu)(wu)(wu)質無(wu)明顯(xian)(xian)(xian)交(jiao)叉(cha)(cha)(cha)反(fan)應。由(you)于受到(dao)(dao)(dao)技術(shu)及(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)來源的(de)(de)(de)限制，不(bu)(bu)可能(neng)完成所有(you)(you)(you)相(xiang)(xiang)關或相(xiang)(xiang)似(si)物(wu)(wu)(wu)質交(jiao)叉(cha)(cha)(cha)反(fan)應檢(jian)測(ce)(ce)(ce)，因此(ci)(ci)本(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)有(you)(you)(you)可能(neng)與(yu)(yu)未(wei)經(jing)檢(jian)測(ce)(ce)(ce)的(de)(de)(de)其(qi)它(ta)物(wu)(wu)(wu)質有(you)(you)(you)交(jiao)叉(cha)(cha)(cha)反(fan)應。 【穩定(ding)(ding)(ding)(ding)(ding)(ding)性(xing)】試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)在(zai)(zai)(zai)有(you)(you)(you)效期內務必按(an)推薦(jian)溫(wen)度(du)(du)(du)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)，活性(xing)降低率(lv)小(xiao)(xiao)于5%。為(wei)(wei)(wei)減小(xiao)(xiao)外部因素(su)(su)對試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)破壞(huai)前后檢(jian)測(ce)(ce)(ce)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)影響(xiang)，實(shi)(shi)驗室(shi)的(de)(de)(de)環境條(tiao)件(jian)需盡(jin)量(liang)(liang)保(bao)(bao)(bao)(bao)持一(yi)(yi)致(zhi)，尤其(qi)是(shi)實(shi)(shi)驗室(shi)內溫(wen)度(du)(du)(du)、濕度(du)(du)(du)及(ji)(ji)溫(wen)育條(tiao)件(jian)。其(qi)次(ci)由(you)同(tong)(tong)(tong)一(yi)(yi)實(shi)(shi)驗員(yuan)來進(jin)(jin)行操(cao)作(zuo)(zuo)可減少人為(wei)(wei)(wei)誤(wu)(wu)差(cha)(cha)。
牛Ⅲ型纖連(lian)蛋(dan)(dan)白域(yu)蛋(dan)(dan)白5(FNDC5)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)中(zhong)(zhong)(zhong)(zhong)文(wen)名稱牛Ⅲ型纖連(lian)蛋(dan)(dan)白域(yu)蛋(dan)(dan)白5(FNDC5)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)規(gui)格96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)類型血(xue)清、血(xue)漿、細胞(bao)上(shang)清液(ye)(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)(ye)、體(ti)(ti)液(ye)(ye)(ye)(ye)(ye)、灌洗(xi)(xi)液(ye)(ye)(ye)(ye)(ye)等(deng)使(shi)(shi)(shi)用(yong)范圍科(ke)研(yan)實驗(yan)，不(bu)得用(yong)于(yu)臨床診斷反應(ying)時(shi)間(jian)1-5h試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)保(bao)(bao)存2-8℃有(you)(you)效期(qi)6個月貨期(qi)3-5天(tian)【操作(zuo)(zuo)步驟】1. 標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)稀(xi)(xi)釋(shi)(shi)(shi)(shi)與(yu)(yu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)(zai)酶(mei)標(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)(ban)(ban)(ban)上(shang)設標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)(zai)第(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)100μl，然(ran)后(hou)(hou)(hou)(hou)(hou)在(zai)(zai)(zai)第(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)；然(ran)后(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)(qu)(qu)100μl分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)第(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)三、第(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)；然(ran)后(hou)(hou)(hou)(hou)(hou)在(zai)(zai)(zai)第(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)第(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)先(xian)各(ge)(ge)取(qu)(qu)(qu)50μl棄(qi)掉(diao)，再(zai)(zai)各(ge)(ge)取(qu)(qu)(qu)50μl分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)六(liu)(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)六(liu)(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50ul，混(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)；混(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)后(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)六(liu)(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)(qu)(qu)50μl分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)后(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)取(qu)(qu)(qu)50μl加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)九(jiu)、第(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)后(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)(qu)(qu)50μl棄(qi)掉(diao)。（稀(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)量(liang)都(dou)為(wei)(wei)(wei)(wei)50μl，濃(nong)度(du)(du)(du)(du)(du)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)為(wei)(wei)(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)設空(kong)(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)(kong)白對照(zhao)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)及(ji)(ji)酶(mei)標(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，其(qi)余各(ge)(ge)步操作(zuo)(zuo)相(xiang)(xiang)同(tong)(tong)）、待測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)(zai)酶(mei)標(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)(ban)(ban)(ban)上(shang)待測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)40μl，然(ran)后(hou)(hou)(hou)(hou)(hou)再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)待測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)＊終稀(xi)(xi)釋(shi)(shi)(shi)(shi)度(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)5倍(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)酶(mei)標(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底(di)(di)部，盡量(liang)不(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)輕(qing)晃動(dong)混(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)。3. 溫(wen)(wen)育：用(yong)封板(ban)(ban)(ban)(ban)膜(mo)封板(ban)(ban)(ban)(ban)后(hou)(hou)(hou)(hou)(hou)置37℃溫(wen)(wen)育30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘。4. 配液(ye)(ye)(ye)(ye)(ye)：將(jiang)(jiang)30（48T的(de)(de)20倍(bei)(bei)）倍(bei)(bei)濃(nong)縮洗(xi)(xi)滌液(ye)(ye)(ye)(ye)(ye)用(yong)蒸餾水(shui)30（48T的(de)(de)20倍(bei)(bei)）倍(bei)(bei)稀(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)備用(yong)。5. 洗(xi)(xi)滌：小心(xin)揭掉(diao)封板(ban)(ban)(ban)(ban)膜(mo)，棄(qi)去(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，甩干，每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)滿洗(xi)(xi)滌液(ye)(ye)(ye)(ye)(ye)，靜置30秒(miao)后(hou)(hou)(hou)(hou)(hou)棄(qi)去(qu)(qu)，如此(ci)重復5次(ci)(ci)，拍干。6. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)酶(mei)標(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)50μl，空(kong)(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外。7. 溫(wen)(wen)育：操作(zuo)(zuo)同(tong)(tong)3。8. 洗(xi)(xi)滌：操作(zuo)(zuo)同(tong)(tong)5。9. 顯(xian)色(se)(se)(se)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)顯(xian)色(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)A50μl，再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)顯(xian)色(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)B50μl，輕(qing)輕(qing)震蕩混(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)，37℃避(bi)光顯(xian)色(se)(se)(se)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘. 10. 終止(zhi)(zhi)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)50μl，終止(zhi)(zhi)反應(ying)（此(ci)時(shi)藍色(se)(se)(se)立(li)轉黃色(se)(se)(se)）。11. 測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)：以(yi)(yi)(yi)空(kong)(kong)白空(kong)(kong)調(diao)零，450nm波長(chang)依序(xu)測(ce)(ce)(ce)(ce)量(liang)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)吸光度(du)(du)(du)(du)(du)（OD值(zhi)(zhi)）。 測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)應(ying)在(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘以(yi)(yi)(yi)內(nei)進(jin)行(xing)(xing)。牛Ⅲ型纖連(lian)蛋(dan)(dan)白域(yu)蛋(dan)(dan)白5(FNDC5)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)【注(zhu)意(yi)(yi)事項(xiang)】1． 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)從(cong)冷藏環境(jing)中(zhong)(zhong)(zhong)(zhong)取(qu)(qu)(qu)出(chu)應(ying)在(zai)(zai)(zai)室(shi)溫(wen)(wen)平(ping)衡15-30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘后(hou)(hou)(hou)(hou)(hou)方可(ke)使(shi)(shi)(shi)用(yong)，酶(mei)標(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)(ban)(ban)(ban)開封后(hou)(hou)(hou)(hou)(hou)如未用(yong)完，板(ban)(ban)(ban)(ban)條(tiao)(tiao)應(ying)裝(zhuang)入(ru)(ru)(ru)(ru)密(mi)(mi)封袋中(zhong)(zhong)(zhong)(zhong)保(bao)(bao)存。2． 濃(nong)洗(xi)(xi)滌液(ye)(ye)(ye)(ye)(ye)可(ke)能(neng)會有(you)(you)結晶析(xi)出(chu)，稀(xi)(xi)釋(shi)(shi)(shi)(shi)時(shi)可(ke)在(zai)(zai)(zai)水(shui)浴中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)溫(wen)(wen)助溶，洗(xi)(xi)滌時(shi)不(bu)影響(xiang)結果。3． 各(ge)(ge)步加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)均應(ying)使(shi)(shi)(shi)用(yong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)器，并經常校對其(qi)準(zhun)(zhun)(zhun)確性(xing)(xing)(xing)，以(yi)(yi)(yi)避(bi)免試(shi)(shi)(shi)(shi)驗(yan)誤(wu)差。一(yi)(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)時(shi)間(jian)＊好控制在(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘內(nei)，如標(biao)(biao)(biao)(biao)本(ben)(ben)數(shu)(shu)量(liang)多(duo)，推薦使(shi)(shi)(shi)用(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)。4． 請每(mei)次(ci)(ci)測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)的(de)(de)同(tong)(tong)時(shi)做標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲線，＊好做復孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如標(biao)(biao)(biao)(biao)本(ben)(ben)中(zhong)(zhong)(zhong)(zhong)待測(ce)(ce)(ce)(ce)物(wu)(wu)質(zhi)含量(liang)過高（樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)OD值(zhi)(zhi)大于(yu)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)OD值(zhi)(zhi)），請先(xian)用(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)釋(shi)(shi)(shi)(shi)一(yi)(yi)定(ding)(ding)(ding)(ding)(ding)倍(bei)(bei)數(shu)(shu)（n倍(bei)(bei)）后(hou)(hou)(hou)(hou)(hou)再(zai)(zai)測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)，計(ji)(ji)算時(shi)請＊后(hou)(hou)(hou)(hou)(hou)乘以(yi)(yi)(yi)總稀(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)（×n×5）。5． 封板(ban)(ban)(ban)(ban)膜(mo)只限(xian)一(yi)(yi)次(ci)(ci)性(xing)(xing)(xing)使(shi)(shi)(shi)用(yong)，以(yi)(yi)(yi)避(bi)免交叉污染。6． 底(di)(di)物(wu)(wu)請避(bi)光保(bao)(bao)存。7． 嚴(yan)格按(an)(an)照(zhao)說明書(shu)的(de)(de)操作(zuo)(zuo)進(jin)行(xing)(xing)，試(shi)(shi)(shi)(shi)驗(yan)結果判(pan)定(ding)(ding)(ding)(ding)(ding)必須(xu)以(yi)(yi)(yi)酶(mei)標(biao)(biao)(biao)(biao)儀讀數(shu)(shu)為(wei)(wei)(wei)(wei)準(zhun)(zhun)(zhun).8． 所(suo)有(you)(you)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)，洗(xi)(xi)滌液(ye)(ye)(ye)(ye)(ye)和(he)各(ge)(ge)種(zhong)廢(fei)棄(qi)物(wu)(wu)都(dou)應(ying)按(an)(an)傳染物(wu)(wu)處理(li)。9． 本(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)不(bu)同(tong)(tong)批號組分(fen)(fen)(fen)(fen)(fen)(fen)(fen)不(bu)得混(hun)(hun)(hun)用(yong)。10. 如與(yu)(yu)英(ying)文(wen)說明書(shu)有(you)(you)異(yi)，以(yi)(yi)(yi)英(ying)文(wen)說明書(shu)為(wei)(wei)(wei)(wei)準(zhun)(zhun)(zhun)。【計(ji)(ji)算】 以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)濃(nong)度(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)橫(heng)坐標(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐標(biao)(biao)(biao)(biao)， 在(zai)(zai)(zai)坐標(biao)(biao)(biao)(biao)紙上(shang)繪出(chu)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲線，根據樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)OD 值(zhi)(zhi)由標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲線查出(chu)相(xiang)(xiang)應(ying)的(de)(de)濃(nong)度(du)(du)(du)(du)(du)；再(zai)(zai)乘以(yi)(yi)(yi)稀(xi)(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)數(shu)(shu)；或用(yong)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)濃(nong)度(du)(du)(du)(du)(du)與(yu)(yu)OD值(zhi)(zhi)計(ji)(ji)算出(chu)標(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)曲線的(de)(de)直(zhi)線回歸方程式(shi)，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)OD值(zhi)(zhi) 代入(ru)(ru)(ru)(ru)方程式(shi)，計(ji)(ji)算出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)度(du)(du)(du)(du)(du)，再(zai)(zai)乘以(yi)(yi)(yi)稀(xi)(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)數(shu)(shu)，即為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)實際濃(nong)度(du)(du)(du)(du)(du)。 【試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)組成】試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)組成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置保(bao)(bao)存說明書(shu)1份1份密(mi)(mi)封袋1個1個封板(ban)(ban)(ban)(ban)膜(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)(tiao)2片（96）2-8℃保(bao)(bao)存微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)標(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)(tiao)2-8℃保(bao)(bao)存標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管(guan)0.3mL*6管(guan)2-8℃保(bao)(bao)存標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)1.5ml×1瓶(ping)2-8℃保(bao)(bao)存酶(mei)標(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)(bao)存樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)(bao)存顯(xian)色(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)(bao)存顯(xian)色(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)(bao)存終止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)3ml×1瓶(ping)6ml×1瓶(ping)2-8℃保(bao)(bao)存注(zhu)意(yi)(yi)：使(shi)(shi)(shi)用(yong)前請檢(jian)(jian)查試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)中(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)的(de)(de)標(biao)(biao)(biao)(biao)簽和(he)數(shu)(shu)量(liang)與(yu)(yu)表(biao)格是否一(yi)(yi)致(zhi)。【精密(mi)(mi)度(du)(du)(du)(du)(du)】精密(mi)(mi)度(du)(du)(du)(du)(du)用(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)的(de)(de)變(bian)異(yi)系(xi)數(shu)(shu)CV 表(biao)示(shi)。CV（%） = SD/mean×100批內(nei)差：取(qu)(qu)(qu)同(tong)(tong)批次(ci)(ci)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)對低(di)、中(zhong)(zhong)(zhong)(zhong)、高值(zhi)(zhi)定(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)進(jin)行(xing)(xing)定(ding)(ding)(ding)(ding)(ding)量(liang)檢(jian)(jian)測(ce)(ce)(ce)(ce)，每(mei)份樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)連(lian)續測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)20 次(ci)(ci)，分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)計(ji)(ji)算不(bu)同(tong)(tong)濃(nong)度(du)(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)平(ping)均值(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)。批內(nei)差： CV<9%批間(jian)差：選取(qu)(qu)(qu)3 個不(bu)同(tong)(tong)批次(ci)(ci)的(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)對低(di)、中(zhong)(zhong)(zhong)(zhong)、高值(zhi)(zhi)定(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)進(jin)行(xing)(xing)定(ding)(ding)(ding)(ding)(ding)量(liang)測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)，每(mei)個樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)使(shi)(shi)(shi)用(yong)同(tong)(tong)一(yi)(yi)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)重復測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)10 次(ci)(ci)，分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)計(ji)(ji)算不(bu)同(tong)(tong)濃(nong)度(du)(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)平(ping)均值(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)。批間(jian)差： CV<11% 【特異(yi)性(xing)(xing)(xing)】本(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)識別(bie)(bie)(bie)天(tian)然(ran)和(he)重組微(wei)生物(wu)(wu)硝酸鹽還原酶(mei)（Nar），經檢(jian)(jian)測(ce)(ce)(ce)(ce)與(yu)(yu)其(qi)它相(xiang)(xiang)似物(wu)(wu)質(zhi)無明顯(xian)交叉反應(ying)。由于(yu)受到(dao)技術及(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)來(lai)源的(de)(de)限(xian)制，不(bu)可(ke)能(neng)完成所(suo)有(you)(you)相(xiang)(xiang)關(guan)或相(xiang)(xiang)似物(wu)(wu)質(zhi)交叉反應(ying)檢(jian)(jian)測(ce)(ce)(ce)(ce)，因(yin)(yin)此(ci)本(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)有(you)(you)可(ke)能(neng)與(yu)(yu)未經檢(jian)(jian)測(ce)(ce)(ce)(ce)的(de)(de)其(qi)它物(wu)(wu)質(zhi)有(you)(you)交叉反應(ying)。 【穩定(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)】試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)在(zai)(zai)(zai)有(you)(you)效期(qi)內(nei)務(wu)必按(an)(an)推薦溫(wen)(wen)度(du)(du)(du)(du)(du)保(bao)(bao)存，活性(xing)(xing)(xing)降低(di)率小于(yu)5%。為(wei)(wei)(wei)(wei)減(jian)小外部因(yin)(yin)素對試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)破壞前后(hou)(hou)(hou)(hou)(hou)檢(jian)(jian)測(ce)(ce)(ce)(ce)值(zhi)(zhi)的(de)(de)影響(xiang)，實驗(yan)室(shi)的(de)(de)環境(jing)條(tiao)(tiao)件(jian)需盡量(liang)保(bao)(bao)持一(yi)(yi)致(zhi)，尤其(qi)是實驗(yan)室(shi)內(nei)溫(wen)(wen)度(du)(du)(du)(du)(du)、濕度(du)(du)(du)(du)(du)及(ji)(ji)溫(wen)(wen)育條(tiao)(tiao)件(jian)。其(qi)次(ci)(ci)由同(tong)(tong)一(yi)(yi)實驗(yan)員來(lai)進(jin)行(xing)(xing)操作(zuo)(zuo)可(ke)減(jian)少人為(wei)(wei)(wei)(wei)誤(wu)差。
牛α-生育(yu)酚轉(zhuan)運(yun)蛋白(bai)(TTPα)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)文名稱牛α-生育(yu)酚轉(zhuan)運(yun)蛋白(bai)(TTPα)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)規(gui)格96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)類(lei)型血清(qing)、血漿、細胞上(shang)清(qing)液(ye)(ye)、尿液(ye)(ye)、體液(ye)(ye)、灌洗(xi)(xi)(xi)液(ye)(ye)等使(shi)用(yong)(yong)(yong)(yong)范(fan)圍科(ke)研(yan)實(shi)驗，不(bu)(bu)得用(yong)(yong)(yong)(yong)于(yu)臨床(chuang)診(zhen)斷反(fan)(fan)應(ying)(ying)(ying)時(shi)間1-5h試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)保(bao)存(cun)2-8℃有(you)效期6個月(yue)貨期3-5天【操(cao)(cao)作(zuo)(zuo)步(bu)驟(zou)】1. 標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)的(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)與(yu)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)酶(mei)(mei)標(biao)(biao)(biao)(biao)包被板(ban)(ban)上(shang)設標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)第(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)100μl，然后(hou)(hou)(hou)(hou)(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)50μl，混(hun)勻；然后(hou)(hou)(hou)(hou)(hou)(hou)(hou)從第(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)各(ge)(ge)(ge)取(qu)(qu)100μl分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)第(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)50μl，混(hun)勻；然后(hou)(hou)(hou)(hou)(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)第(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)先(xian)各(ge)(ge)(ge)取(qu)(qu)50μl棄(qi)(qi)掉，再(zai)(zai)各(ge)(ge)(ge)取(qu)(qu)50μl分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)五、第(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，再(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)五、第(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)50ul，混(hun)勻；混(hun)勻后(hou)(hou)(hou)(hou)(hou)(hou)(hou)從第(di)(di)(di)(di)五、第(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)各(ge)(ge)(ge)取(qu)(qu)50μl分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，再(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)50μl，混(hun)勻后(hou)(hou)(hou)(hou)(hou)(hou)(hou)從第(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)取(qu)(qu)50μl加(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)九(jiu)、第(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，再(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)50μl，混(hun)勻后(hou)(hou)(hou)(hou)(hou)(hou)(hou)從第(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)各(ge)(ge)(ge)取(qu)(qu)50μl棄(qi)(qi)掉。（稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)各(ge)(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)都(dou)為(wei)(wei)50μl，濃(nong)度(du)(du)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)為(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)設空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空白(bai)對(dui)照(zhao)(zhao)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)及(ji)(ji)酶(mei)(mei)標(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，其(qi)余(yu)各(ge)(ge)(ge)步(bu)操(cao)(cao)作(zuo)(zuo)相(xiang)同(tong)）、待(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)酶(mei)(mei)標(biao)(biao)(biao)(biao)包被板(ban)(ban)上(shang)待(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)40μl，然后(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)待(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)＊終稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)度(du)(du)為(wei)(wei)5倍）。加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)于(yu)酶(mei)(mei)標(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部，盡量(liang)不(bu)(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)(qing)輕(qing)(qing)晃動(dong)混(hun)勻。3. 溫(wen)(wen)育(yu)：用(yong)(yong)(yong)(yong)封(feng)(feng)板(ban)(ban)膜(mo)(mo)封(feng)(feng)板(ban)(ban)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)置(zhi)(zhi)37℃溫(wen)(wen)育(yu)30分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)。4. 配液(ye)(ye)：將(jiang)30（48T的(de)(de)(de)(de)20倍）倍濃(nong)縮洗(xi)(xi)(xi)滌(di)(di)液(ye)(ye)用(yong)(yong)(yong)(yong)蒸餾水30（48T的(de)(de)(de)(de)20倍）倍稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)備用(yong)(yong)(yong)(yong)。5. 洗(xi)(xi)(xi)滌(di)(di)：小心揭掉封(feng)(feng)板(ban)(ban)膜(mo)(mo)，棄(qi)(qi)去液(ye)(ye)體，甩干(gan)(gan)，每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)滌(di)(di)液(ye)(ye)，靜置(zhi)(zhi)30秒后(hou)(hou)(hou)(hou)(hou)(hou)(hou)棄(qi)(qi)去，如此(ci)重(zhong)復(fu)5次(ci)，拍干(gan)(gan)。6. 加(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)入酶(mei)(mei)標(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)50μl，空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除(chu)外(wai)。7. 溫(wen)(wen)育(yu)：操(cao)(cao)作(zuo)(zuo)同(tong)3。8. 洗(xi)(xi)(xi)滌(di)(di)：操(cao)(cao)作(zuo)(zuo)同(tong)5。9. 顯(xian)色(se)(se)(se)：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)入顯(xian)色(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)A50μl，再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)入顯(xian)色(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)B50μl，輕(qing)(qing)輕(qing)(qing)震蕩混(hun)勻，37℃避光(guang)顯(xian)色(se)(se)(se)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong). 10. 終止：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)終止液(ye)(ye)50μl，終止反(fan)(fan)應(ying)(ying)(ying)（此(ci)時(shi)藍色(se)(se)(se)立(li)轉(zhuan)黃色(se)(se)(se)）。11. 測(ce)(ce)(ce)(ce)定(ding)(ding)：以(yi)空白(bai)空調零，450nm波(bo)長依序測(ce)(ce)(ce)(ce)量(liang)各(ge)(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)吸光(guang)度(du)(du)（OD值(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)(ce)定(ding)(ding)應(ying)(ying)(ying)在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)終止液(ye)(ye)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)以(yi)內(nei)進(jin)行(xing)(xing)。牛α-生育(yu)酚轉(zhuan)運(yun)蛋白(bai)(TTPα)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)【注(zhu)意(yi)事項】1． 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)從冷藏環(huan)境(jing)中(zhong)(zhong)取(qu)(qu)出(chu)應(ying)(ying)(ying)在(zai)(zai)室(shi)溫(wen)(wen)平衡15-30分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)方(fang)可(ke)使(shi)用(yong)(yong)(yong)(yong)，酶(mei)(mei)標(biao)(biao)(biao)(biao)包被板(ban)(ban)開封(feng)(feng)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)如未用(yong)(yong)(yong)(yong)完，板(ban)(ban)條(tiao)應(ying)(ying)(ying)裝入密(mi)(mi)封(feng)(feng)袋(dai)中(zhong)(zhong)保(bao)存(cun)。2． 濃(nong)洗(xi)(xi)(xi)滌(di)(di)液(ye)(ye)可(ke)能會有(you)結晶析出(chu)，稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)時(shi)可(ke)在(zai)(zai)水浴中(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)溫(wen)(wen)助溶，洗(xi)(xi)(xi)滌(di)(di)時(shi)不(bu)(bu)影響(xiang)結果(guo)(guo)。3． 各(ge)(ge)(ge)步(bu)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)均(jun)應(ying)(ying)(ying)使(shi)用(yong)(yong)(yong)(yong)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)器，并(bing)經常校對(dui)其(qi)準(zhun)(zhun)確性，以(yi)避免試(shi)(shi)(shi)(shi)驗誤(wu)(wu)差。一(yi)(yi)次(ci)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)間＊好控制(zhi)在(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)內(nei)，如標(biao)(biao)(biao)(biao)本(ben)(ben)(ben)數(shu)(shu)(shu)(shu)量(liang)多(duo)，推薦使(shi)用(yong)(yong)(yong)(yong)排槍(qiang)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4． 請每次(ci)測(ce)(ce)(ce)(ce)定(ding)(ding)的(de)(de)(de)(de)同(tong)時(shi)做標(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)線(xian)，＊好做復(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如標(biao)(biao)(biao)(biao)本(ben)(ben)(ben)中(zhong)(zhong)待(dai)測(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)質含量(liang)過高（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)OD值(zhi)(zhi)(zhi)大于(yu)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)），請先(xian)用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)定(ding)(ding)倍數(shu)(shu)(shu)(shu)（n倍）后(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)(zai)測(ce)(ce)(ce)(ce)定(ding)(ding)，計(ji)算(suan)(suan)(suan)時(shi)請＊后(hou)(hou)(hou)(hou)(hou)(hou)(hou)乘以(yi)總稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)（×n×5）。5． 封(feng)(feng)板(ban)(ban)膜(mo)(mo)只限一(yi)(yi)次(ci)性使(shi)用(yong)(yong)(yong)(yong)，以(yi)避免交叉(cha)污染(ran)。6． 底物(wu)(wu)(wu)(wu)請避光(guang)保(bao)存(cun)。7． 嚴格按(an)照(zhao)(zhao)說明(ming)(ming)書的(de)(de)(de)(de)操(cao)(cao)作(zuo)(zuo)進(jin)行(xing)(xing)，試(shi)(shi)(shi)(shi)驗結果(guo)(guo)判定(ding)(ding)必(bi)須以(yi)酶(mei)(mei)標(biao)(biao)(biao)(biao)儀(yi)讀數(shu)(shu)(shu)(shu)為(wei)(wei)準(zhun)(zhun).8． 所(suo)(suo)有(you)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)，洗(xi)(xi)(xi)滌(di)(di)液(ye)(ye)和(he)各(ge)(ge)(ge)種廢棄(qi)(qi)物(wu)(wu)(wu)(wu)都(dou)應(ying)(ying)(ying)按(an)傳(chuan)染(ran)物(wu)(wu)(wu)(wu)處(chu)理。9． 本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)不(bu)(bu)同(tong)批(pi)(pi)號(hao)組(zu)(zu)分(fen)(fen)(fen)(fen)(fen)(fen)不(bu)(bu)得混(hun)用(yong)(yong)(yong)(yong)。10. 如與(yu)英(ying)文說明(ming)(ming)書有(you)異(yi)(yi)，以(yi)英(ying)文說明(ming)(ming)書為(wei)(wei)準(zhun)(zhun)。【計(ji)算(suan)(suan)(suan)】 以(yi)標(biao)(biao)(biao)(biao)準(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)濃(nong)度(du)(du)為(wei)(wei)橫坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)縱坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)， 在(zai)(zai)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)紙上(shang)繪出(chu)標(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)線(xian)，根據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)的(de)(de)(de)(de)OD 值(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)線(xian)查(cha)出(chu)相(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)濃(nong)度(du)(du)；再(zai)(zai)乘以(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi) 倍數(shu)(shu)(shu)(shu)；或用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)準(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)濃(nong)度(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)算(suan)(suan)(suan)出(chu)標(biao)(biao)(biao)(biao) 準(zhun)(zhun)曲(qu)線(xian)的(de)(de)(de)(de)直(zhi)線(xian)回歸方(fang)程式，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)的(de)(de)(de)(de)OD值(zhi)(zhi)(zhi) 代入方(fang)程式，計(ji)算(suan)(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)濃(nong)度(du)(du)，再(zai)(zai)乘以(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi) 倍數(shu)(shu)(shu)(shu)，即為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)的(de)(de)(de)(de)實(shi)際濃(nong)度(du)(du)。 【試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)組(zu)(zu)成】試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)組(zu)(zu)成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)(zhi)保(bao)存(cun)說明(ming)(ming)書1份1份密(mi)(mi)封(feng)(feng)袋(dai)1個1個封(feng)(feng)板(ban)(ban)膜(mo)(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)2片(pian)（96）2-8℃保(bao)存(cun)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)標(biao)(biao)(biao)(biao)板(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)2-8℃保(bao)存(cun)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)存(cun)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)2-8℃保(bao)存(cun)酶(mei)(mei)標(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)存(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)存(cun)顯(xian)色(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)存(cun)顯(xian)色(se)(se)(se)劑(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)存(cun)終止液(ye)(ye)3ml×1瓶(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)2-8℃保(bao)存(cun)注(zhu)意(yi)：使(shi)用(yong)(yong)(yong)(yong)前請檢查(cha)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)標(biao)(biao)(biao)(biao)簽和(he)數(shu)(shu)(shu)(shu)量(liang)與(yu)表格是否一(yi)(yi)致。【精密(mi)(mi)度(du)(du)】精密(mi)(mi)度(du)(du)用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)測(ce)(ce)(ce)(ce)定(ding)(ding)值(zhi)(zhi)(zhi)的(de)(de)(de)(de)變異(yi)(yi)系數(shu)(shu)(shu)(shu)CV 表示。CV（%） = SD/mean×100批(pi)(pi)內(nei)差：取(qu)(qu)同(tong)批(pi)(pi)次(ci)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)對(dui)低(di)(di)(di)、中(zhong)(zhong)、高值(zhi)(zhi)(zhi)定(ding)(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)進(jin)行(xing)(xing)定(ding)(ding)量(liang)檢測(ce)(ce)(ce)(ce)，每份樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)連(lian)續測(ce)(ce)(ce)(ce)定(ding)(ding)20 次(ci)，分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)計(ji)算(suan)(suan)(suan)不(bu)(bu)同(tong)濃(nong)度(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)平均(jun)值(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)。批(pi)(pi)內(nei)差： CV<9%批(pi)(pi)間差：選取(qu)(qu)3 個不(bu)(bu)同(tong)批(pi)(pi)次(ci)的(de)(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)對(dui)低(di)(di)(di)、中(zhong)(zhong)、高值(zhi)(zhi)(zhi)定(ding)(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)進(jin)行(xing)(xing)定(ding)(ding)量(liang)測(ce)(ce)(ce)(ce)定(ding)(ding)，每個樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)使(shi)用(yong)(yong)(yong)(yong)同(tong)一(yi)(yi)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)重(zhong)復(fu)測(ce)(ce)(ce)(ce)定(ding)(ding)10 次(ci)，分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)計(ji)算(suan)(suan)(suan)不(bu)(bu)同(tong)濃(nong)度(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)平均(jun)值(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)。批(pi)(pi)間差： CV<11% 【特異(yi)(yi)性】本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)識別(bie)(bie)天然和(he)重(zhong)組(zu)(zu)微生物(wu)(wu)(wu)(wu)硝酸鹽還原酶(mei)(mei)（Nar），經檢測(ce)(ce)(ce)(ce)與(yu)其(qi)它相(xiang)似(si)物(wu)(wu)(wu)(wu)質無明(ming)(ming)顯(xian)交叉(cha)反(fan)(fan)應(ying)(ying)(ying)。由(you)于(yu)受到(dao)技術及(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)來(lai)源的(de)(de)(de)(de)限制(zhi)，不(bu)(bu)可(ke)能完成所(suo)(suo)有(you)相(xiang)關(guan)或相(xiang)似(si)物(wu)(wu)(wu)(wu)質交叉(cha)反(fan)(fan)應(ying)(ying)(ying)檢測(ce)(ce)(ce)(ce)，因(yin)此(ci)本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)有(you)可(ke)能與(yu)未經檢測(ce)(ce)(ce)(ce)的(de)(de)(de)(de)其(qi)它物(wu)(wu)(wu)(wu)質有(you)交叉(cha)反(fan)(fan)應(ying)(ying)(ying)。 【穩定(ding)(ding)性】試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)在(zai)(zai)有(you)效期內(nei)務必(bi)按(an)推薦溫(wen)(wen)度(du)(du)保(bao)存(cun)，活性降低(di)(di)(di)率(lv)小于(yu)5%。為(wei)(wei)減小外(wai)部因(yin)素(su)對(dui)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)破壞(huai)前后(hou)(hou)(hou)(hou)(hou)(hou)(hou)檢測(ce)(ce)(ce)(ce)值(zhi)(zhi)(zhi)的(de)(de)(de)(de)影響(xiang)，實(shi)驗室(shi)的(de)(de)(de)(de)環(huan)境(jing)條(tiao)件(jian)需盡量(liang)保(bao)持(chi)一(yi)(yi)致，尤其(qi)是實(shi)驗室(shi)內(nei)溫(wen)(wen)度(du)(du)、濕度(du)(du)及(ji)(ji)溫(wen)(wen)育(yu)條(tiao)件(jian)。其(qi)次(ci)由(you)同(tong)一(yi)(yi)實(shi)驗員(yuan)來(lai)進(jin)行(xing)(xing)操(cao)(cao)作(zuo)(zuo)可(ke)減少人為(wei)(wei)誤(wu)(wu)差。
牛皮膚衍(yan)(yan)生肽酶(mei)(mei)(mei)(mei)抑制(zhi)劑(ji)(ji)(ji)(ji)(ji)(ji)3(PI3/Elafin)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)(zhong)文名(ming)稱牛皮膚衍(yan)(yan)生肽酶(mei)(mei)(mei)(mei)抑制(zhi)劑(ji)(ji)(ji)(ji)(ji)(ji)3(PI3/Elafin)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)規格96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)類型血清(qing)、血漿、細胞(bao)上清(qing)液(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)、體(ti)液(ye)(ye)(ye)(ye)、灌洗(xi)(xi)(xi)(xi)液(ye)(ye)(ye)(ye)等(deng)使(shi)用(yong)(yong)范(fan)圍科研實驗(yan)，不(bu)(bu)(bu)得用(yong)(yong)于臨(lin)床(chuang)診(zhen)斷反(fan)(fan)應(ying)(ying)時(shi)間(jian)1-5h試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)保(bao)(bao)(bao)存2-8℃有(you)(you)效(xiao)期6個(ge)月(yue)貨期3-5天(tian)【操(cao)作(zuo)(zuo)步驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的稀(xi)釋(shi)(shi)(shi)(shi)與(yu)(yu)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)(ban)上設標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)第(di)(di)(di)一(yi)、第(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，然后(hou)在(zai)第(di)(di)(di)一(yi)、第(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)；然后(hou)從第(di)(di)(di)一(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)各(ge)(ge)取(qu)(qu)100μl分(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)第(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)(zai)在(zai)第(di)(di)(di)三(san)、第(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)；然后(hou)在(zai)第(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)第(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)先各(ge)(ge)取(qu)(qu)50μl棄掉(diao)(diao)，再(zai)(zai)(zai)各(ge)(ge)取(qu)(qu)50μl分(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)五(wu)、第(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，再(zai)(zai)(zai)在(zai)第(di)(di)(di)五(wu)、第(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)50ul，混(hun)勻(yun)；混(hun)勻(yun)后(hou)從第(di)(di)(di)五(wu)、第(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)各(ge)(ge)取(qu)(qu)50μl分(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)七(qi)、第(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，再(zai)(zai)(zai)在(zai)第(di)(di)(di)七(qi)、第(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)后(hou)從第(di)(di)(di)七(qi)、第(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)取(qu)(qu)50μl加(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)九(jiu)、第(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，再(zai)(zai)(zai)在(zai)第(di)(di)(di)九(jiu)第(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)后(hou)從第(di)(di)(di)九(jiu)第(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)各(ge)(ge)取(qu)(qu)50μl棄掉(diao)(diao)。（稀(xi)釋(shi)(shi)(shi)(shi)后(hou)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)都為(wei)(wei)(wei)50μl，濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)分(fen)(fen)別(bie)(bie)為(wei)(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)別(bie)(bie)設空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空白(bai)對(dui)照孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)及(ji)(ji)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，其(qi)余(yu)各(ge)(ge)步操(cao)作(zuo)(zuo)相(xiang)同(tong)(tong)(tong)(tong)(tong)）、待(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)(ban)上待(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)先加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)40μl，然后(hou)再(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)待(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)＊終稀(xi)釋(shi)(shi)(shi)(shi)度(du)(du)(du)(du)(du)為(wei)(wei)(wei)5倍(bei)(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)于酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底(di)(di)部(bu)，盡量(liang)(liang)不(bu)(bu)(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁(bi)，輕(qing)輕(qing)晃(huang)動混(hun)勻(yun)。3. 溫育(yu)：用(yong)(yong)封(feng)(feng)(feng)板(ban)(ban)(ban)膜(mo)(mo)(mo)封(feng)(feng)(feng)板(ban)(ban)(ban)后(hou)置37℃溫育(yu)30分(fen)(fen)鐘。4. 配(pei)液(ye)(ye)(ye)(ye)：將(jiang)30（48T的20倍(bei)(bei)(bei)）倍(bei)(bei)(bei)濃(nong)(nong)(nong)縮(suo)洗(xi)(xi)(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)用(yong)(yong)蒸餾水(shui)30（48T的20倍(bei)(bei)(bei)）倍(bei)(bei)(bei)稀(xi)釋(shi)(shi)(shi)(shi)后(hou)備用(yong)(yong)。5. 洗(xi)(xi)(xi)(xi)滌(di)：小心揭掉(diao)(diao)封(feng)(feng)(feng)板(ban)(ban)(ban)膜(mo)(mo)(mo)，棄去(qu)液(ye)(ye)(ye)(ye)體(ti)，甩(shuai)干(gan)，每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)，靜置30秒后(hou)棄去(qu)，如(ru)此重(zhong)復5次(ci)(ci)(ci)，拍干(gan)。6. 加(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)(mei)(mei)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)入酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)50μl，空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外。7. 溫育(yu)：操(cao)作(zuo)(zuo)同(tong)(tong)(tong)(tong)(tong)3。8. 洗(xi)(xi)(xi)(xi)滌(di)：操(cao)作(zuo)(zuo)同(tong)(tong)(tong)(tong)(tong)5。9. 顯(xian)色(se)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)(jia)(jia)入顯(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)A50μl，再(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)入顯(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)B50μl，輕(qing)輕(qing)震蕩混(hun)勻(yun)，37℃避(bi)(bi)光(guang)(guang)顯(xian)色(se)15分(fen)(fen)鐘. 10. 終止(zhi)(zhi)(zhi)(zhi)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)終止(zhi)(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)50μl，終止(zhi)(zhi)(zhi)(zhi)反(fan)(fan)應(ying)(ying)（此時(shi)藍色(se)立轉黃(huang)色(se)）。11. 測(ce)(ce)(ce)定(ding)(ding)(ding)：以(yi)(yi)空白(bai)空調零，450nm波長依序測(ce)(ce)(ce)量(liang)(liang)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的吸光(guang)(guang)度(du)(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)定(ding)(ding)(ding)應(ying)(ying)在(zai)加(jia)(jia)(jia)(jia)(jia)(jia)終止(zhi)(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)后(hou)15分(fen)(fen)鐘以(yi)(yi)內進(jin)行(xing)(xing)。牛皮膚衍(yan)(yan)生肽酶(mei)(mei)(mei)(mei)抑制(zhi)劑(ji)(ji)(ji)(ji)(ji)(ji)3(PI3/Elafin)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)【注意事項】1． 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)從冷藏環(huan)(huan)境中(zhong)(zhong)(zhong)取(qu)(qu)出(chu)應(ying)(ying)在(zai)室溫平衡15-30分(fen)(fen)鐘后(hou)方可使(shi)用(yong)(yong)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)(ban)開封(feng)(feng)(feng)后(hou)如(ru)未用(yong)(yong)完，板(ban)(ban)(ban)條(tiao)(tiao)應(ying)(ying)裝入密封(feng)(feng)(feng)袋中(zhong)(zhong)(zhong)保(bao)(bao)(bao)存。2． 濃(nong)(nong)(nong)洗(xi)(xi)(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)可能會有(you)(you)結晶(jing)析出(chu)，稀(xi)釋(shi)(shi)(shi)(shi)時(shi)可在(zai)水(shui)浴(yu)中(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)溫助溶，洗(xi)(xi)(xi)(xi)滌(di)時(shi)不(bu)(bu)(bu)影(ying)響結果(guo)。3． 各(ge)(ge)步加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)均應(ying)(ying)使(shi)用(yong)(yong)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)器，并經常(chang)校(xiao)對(dui)其(qi)準(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)，以(yi)(yi)避(bi)(bi)免試(shi)(shi)(shi)(shi)驗(yan)誤差(cha)(cha)(cha)。一(yi)次(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)間(jian)＊好(hao)控制(zhi)在(zai)5分(fen)(fen)鐘內，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)數量(liang)(liang)多，推薦使(shi)用(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4． 請(qing)每(mei)(mei)次(ci)(ci)(ci)測(ce)(ce)(ce)定(ding)(ding)(ding)的同(tong)(tong)(tong)(tong)(tong)時(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)，＊好(hao)做(zuo)復孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)中(zhong)(zhong)(zhong)待(dai)測(ce)(ce)(ce)物(wu)(wu)(wu)質(zhi)(zhi)含量(liang)(liang)過(guo)高(gao)（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)大于標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)一(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)），請(qing)先用(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)稀(xi)釋(shi)(shi)(shi)(shi)一(yi)定(ding)(ding)(ding)倍(bei)(bei)(bei)數（n倍(bei)(bei)(bei)）后(hou)再(zai)(zai)(zai)測(ce)(ce)(ce)定(ding)(ding)(ding)，計(ji)(ji)算(suan)(suan)時(shi)請(qing)＊后(hou)乘以(yi)(yi)總稀(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數（×n×5）。5． 封(feng)(feng)(feng)板(ban)(ban)(ban)膜(mo)(mo)(mo)只限(xian)一(yi)次(ci)(ci)(ci)性(xing)使(shi)用(yong)(yong)，以(yi)(yi)避(bi)(bi)免交(jiao)叉污染。6． 底(di)(di)物(wu)(wu)(wu)請(qing)避(bi)(bi)光(guang)(guang)保(bao)(bao)(bao)存。7． 嚴格按照說明書(shu)(shu)的操(cao)作(zuo)(zuo)進(jin)行(xing)(xing)，試(shi)(shi)(shi)(shi)驗(yan)結果(guo)判定(ding)(ding)(ding)必須以(yi)(yi)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀數為(wei)(wei)(wei)準(zhun)(zhun)(zhun)(zhun)(zhun).8． 所有(you)(you)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)，洗(xi)(xi)(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)和(he)各(ge)(ge)種廢棄物(wu)(wu)(wu)都應(ying)(ying)按傳染物(wu)(wu)(wu)處理。9． 本(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)不(bu)(bu)(bu)同(tong)(tong)(tong)(tong)(tong)批(pi)號組(zu)分(fen)(fen)不(bu)(bu)(bu)得混(hun)用(yong)(yong)。10. 如(ru)與(yu)(yu)英(ying)文說明書(shu)(shu)有(you)(you)異，以(yi)(yi)英(ying)文說明書(shu)(shu)為(wei)(wei)(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)。【計(ji)(ji)算(suan)(suan)】 以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)為(wei)(wei)(wei)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)(wei)(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上繪(hui)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)，根據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的OD 值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)查(cha)出(chu)相(xiang)應(ying)(ying)的濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)；再(zai)(zai)(zai)乘以(yi)(yi)稀(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)(bei)數；或用(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)與(yu)(yu)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)計(ji)(ji)算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)的直線(xian)回歸方程(cheng)式，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi) 代(dai)入方程(cheng)式，計(ji)(ji)算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)，再(zai)(zai)(zai)乘以(yi)(yi)稀(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)(bei)數，即為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的實際濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)。 【試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)組(zu)成】試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)組(zu)成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置保(bao)(bao)(bao)存說明書(shu)(shu)1份(fen)1份(fen)密封(feng)(feng)(feng)袋1個(ge)1個(ge)封(feng)(feng)(feng)板(ban)(ban)(ban)膜(mo)(mo)(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)(tiao)2片(pian)（96）2-8℃保(bao)(bao)(bao)存微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)(tiao)2-8℃保(bao)(bao)(bao)存標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)(bao)存標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存顯(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存顯(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存終止(zhi)(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存注意：使(shi)用(yong)(yong)前(qian)請(qing)檢(jian)(jian)查(cha)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)的標(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和(he)數量(liang)(liang)與(yu)(yu)表格是否一(yi)致(zhi)。【精(jing)密度(du)(du)(du)(du)(du)】精(jing)密度(du)(du)(du)(du)(du)用(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)的變異系數CV 表示。CV（%） = SD/mean×100批(pi)內差(cha)(cha)(cha)：取(qu)(qu)同(tong)(tong)(tong)(tong)(tong)批(pi)次(ci)(ci)(ci)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)對(dui)低、中(zhong)(zhong)(zhong)、高(gao)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)進(jin)行(xing)(xing)定(ding)(ding)(ding)量(liang)(liang)檢(jian)(jian)測(ce)(ce)(ce)，每(mei)(mei)份(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)連續測(ce)(ce)(ce)定(ding)(ding)(ding)20 次(ci)(ci)(ci)，分(fen)(fen)別(bie)(bie)計(ji)(ji)算(suan)(suan)不(bu)(bu)(bu)同(tong)(tong)(tong)(tong)(tong)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)的平均值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。批(pi)內差(cha)(cha)(cha)： CV<9%批(pi)間(jian)差(cha)(cha)(cha)：選取(qu)(qu)3 個(ge)不(bu)(bu)(bu)同(tong)(tong)(tong)(tong)(tong)批(pi)次(ci)(ci)(ci)的試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)分(fen)(fen)別(bie)(bie)對(dui)低、中(zhong)(zhong)(zhong)、高(gao)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)進(jin)行(xing)(xing)定(ding)(ding)(ding)量(liang)(liang)測(ce)(ce)(ce)定(ding)(ding)(ding)，每(mei)(mei)個(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)使(shi)用(yong)(yong)同(tong)(tong)(tong)(tong)(tong)一(yi)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)重(zhong)復測(ce)(ce)(ce)定(ding)(ding)(ding)10 次(ci)(ci)(ci)，分(fen)(fen)別(bie)(bie)計(ji)(ji)算(suan)(suan)不(bu)(bu)(bu)同(tong)(tong)(tong)(tong)(tong)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)的平均值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。批(pi)間(jian)差(cha)(cha)(cha)： CV<11% 【特異性(xing)】本(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)識別(bie)(bie)天(tian)然和(he)重(zhong)組(zu)微生物(wu)(wu)(wu)硝酸(suan)鹽還原(yuan)酶(mei)(mei)(mei)(mei)（Nar），經檢(jian)(jian)測(ce)(ce)(ce)與(yu)(yu)其(qi)它(ta)相(xiang)似(si)物(wu)(wu)(wu)質(zhi)(zhi)無明顯(xian)交(jiao)叉反(fan)(fan)應(ying)(ying)。由于受到(dao)技術(shu)及(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)來源的限(xian)制(zhi)，不(bu)(bu)(bu)可能完成所有(you)(you)相(xiang)關或相(xiang)似(si)物(wu)(wu)(wu)質(zhi)(zhi)交(jiao)叉反(fan)(fan)應(ying)(ying)檢(jian)(jian)測(ce)(ce)(ce)，因此本(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)有(you)(you)可能與(yu)(yu)未經檢(jian)(jian)測(ce)(ce)(ce)的其(qi)它(ta)物(wu)(wu)(wu)質(zhi)(zhi)有(you)(you)交(jiao)叉反(fan)(fan)應(ying)(ying)。 【穩定(ding)(ding)(ding)性(xing)】試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)在(zai)有(you)(you)效(xiao)期內務(wu)必按推薦溫度(du)(du)(du)(du)(du)保(bao)(bao)(bao)存，活性(xing)降低率(lv)小于5%。為(wei)(wei)(wei)減小外部(bu)因素對(dui)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)破壞前(qian)后(hou)檢(jian)(jian)測(ce)(ce)(ce)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)的影(ying)響，實驗(yan)室的環(huan)(huan)境條(tiao)(tiao)件需盡量(liang)(liang)保(bao)(bao)(bao)持一(yi)致(zhi)，尤其(qi)是實驗(yan)室內溫度(du)(du)(du)(du)(du)、濕度(du)(du)(du)(du)(du)及(ji)(ji)溫育(yu)條(tiao)(tiao)件。其(qi)次(ci)(ci)(ci)由同(tong)(tong)(tong)(tong)(tong)一(yi)實驗(yan)員來進(jin)行(xing)(xing)操(cao)作(zuo)(zuo)可減少人(ren)為(wei)(wei)(wei)誤差(cha)(cha)(cha)。
牛絨(rong)毛(mao)(mao)(mao)蛋白1(VIL1)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)文(wen)名稱牛絨(rong)毛(mao)(mao)(mao)蛋白1(VIL1)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)(he)規格96T/48T樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)類型(xing)血清(qing)、血漿、細(xi)胞上(shang)清(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、體液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、灌洗(xi)(xi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)等使用(yong)范圍科(ke)研(yan)實驗(yan)，不(bu)得(de)用(yong)于臨(lin)床診(zhen)斷反(fan)應(ying)(ying)(ying)(ying)時間1-5h試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)(he)保(bao)(bao)存2-8℃有(you)(you)(you)效(xiao)期6個(ge)月貨期3-5天(tian)【操(cao)作(zuo)(zuo)步(bu)驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)稀(xi)釋(shi)(shi)(shi)(shi)與(yu)(yu)(yu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被(bei)板(ban)上(shang)設標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)第(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，然(ran)后(hou)(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)(yun)；然(ran)后(hou)(hou)(hou)(hou)從(cong)(cong)第(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)(qu)(qu)100μl分(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)第(di)(di)(di)(di)(di)四(si)(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)三、第(di)(di)(di)(di)(di)四(si)(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)(yun)；然(ran)后(hou)(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)第(di)(di)(di)(di)(di)四(si)(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)先(xian)各(ge)(ge)取(qu)(qu)(qu)50μl棄掉，再(zai)(zai)(zai)(zai)各(ge)(ge)取(qu)(qu)(qu)50μl分(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50ul，混(hun)(hun)(hun)勻(yun)(yun)；混(hun)(hun)(hun)勻(yun)(yun)后(hou)(hou)(hou)(hou)從(cong)(cong)第(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)(qu)(qu)50μl分(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)(yun)后(hou)(hou)(hou)(hou)從(cong)(cong)第(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)取(qu)(qu)(qu)50μl加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)(di)(di)九(jiu)、第(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)(yun)后(hou)(hou)(hou)(hou)從(cong)(cong)第(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)(qu)(qu)50μl棄掉。（稀(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)量(liang)都為(wei)(wei)50μl，濃度(du)(du)(du)(du)(du)分(fen)(fen)(fen)別(bie)為(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)別(bie)設空(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)白對(dui)(dui)照孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)及酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)，其余各(ge)(ge)步(bu)操(cao)作(zuo)(zuo)相同(tong)）、待(dai)測(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被(bei)板(ban)上(shang)待(dai)測(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)40μl，然(ran)后(hou)(hou)(hou)(hou)再(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)待(dai)測(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)＊終(zhong)稀(xi)釋(shi)(shi)(shi)(shi)度(du)(du)(du)(du)(du)為(wei)(wei)5倍(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)將樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)于酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底(di)部(bu)(bu)，盡量(liang)不(bu)觸及孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)輕(qing)晃動混(hun)(hun)(hun)勻(yun)(yun)。3. 溫(wen)(wen)育(yu)：用(yong)封(feng)(feng)(feng)板(ban)膜封(feng)(feng)(feng)板(ban)后(hou)(hou)(hou)(hou)置(zhi)(zhi)37℃溫(wen)(wen)育(yu)30分(fen)(fen)(fen)鐘。4. 配液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)：將30（48T的(de)(de)(de)(de)20倍(bei)(bei)）倍(bei)(bei)濃縮洗(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)用(yong)蒸(zheng)餾(liu)水(shui)30（48T的(de)(de)(de)(de)20倍(bei)(bei)）倍(bei)(bei)稀(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)備用(yong)。5. 洗(xi)(xi)滌(di)：小心揭掉封(feng)(feng)(feng)板(ban)膜，棄去(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體，甩干(gan)，每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)滿(man)洗(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，靜置(zhi)(zhi)30秒后(hou)(hou)(hou)(hou)棄去(qu)(qu)，如(ru)此重復(fu)5次(ci)(ci)(ci)(ci)，拍干(gan)。6. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)(mei)(mei)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)50μl，空(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除(chu)外。7. 溫(wen)(wen)育(yu)：操(cao)作(zuo)(zuo)同(tong)3。8. 洗(xi)(xi)滌(di)：操(cao)作(zuo)(zuo)同(tong)5。9. 顯(xian)(xian)(xian)色(se)(se)(se)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入顯(xian)(xian)(xian)色(se)(se)(se)劑(ji)(ji)A50μl，再(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入顯(xian)(xian)(xian)色(se)(se)(se)劑(ji)(ji)B50μl，輕(qing)輕(qing)震蕩混(hun)(hun)(hun)勻(yun)(yun)，37℃避(bi)光(guang)顯(xian)(xian)(xian)色(se)(se)(se)15分(fen)(fen)(fen)鐘. 10. 終(zhong)止：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止反(fan)應(ying)(ying)(ying)(ying)（此時藍色(se)(se)(se)立轉黃(huang)色(se)(se)(se)）。11. 測(ce)定(ding)(ding)：以(yi)(yi)(yi)空(kong)白空(kong)調零，450nm波長依序測(ce)量(liang)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)吸光(guang)度(du)(du)(du)(du)(du)（OD值(zhi)）。 測(ce)定(ding)(ding)應(ying)(ying)(ying)(ying)在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)鐘以(yi)(yi)(yi)內(nei)(nei)(nei)進行。牛絨(rong)毛(mao)(mao)(mao)蛋白1(VIL1)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)(he)【注意事(shi)項】1． 試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)(he)從(cong)(cong)冷藏(zang)環(huan)境中(zhong)(zhong)(zhong)(zhong)(zhong)取(qu)(qu)(qu)出(chu)應(ying)(ying)(ying)(ying)在(zai)(zai)室(shi)溫(wen)(wen)平衡15-30分(fen)(fen)(fen)鐘后(hou)(hou)(hou)(hou)方(fang)可(ke)使用(yong)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被(bei)板(ban)開封(feng)(feng)(feng)后(hou)(hou)(hou)(hou)如(ru)未用(yong)完，板(ban)條(tiao)應(ying)(ying)(ying)(ying)裝入密(mi)封(feng)(feng)(feng)袋(dai)(dai)中(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)存。2． 濃洗(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)可(ke)能會有(you)(you)(you)結(jie)晶析出(chu)，稀(xi)釋(shi)(shi)(shi)(shi)時可(ke)在(zai)(zai)水(shui)浴(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)溫(wen)(wen)助溶，洗(xi)(xi)滌(di)時不(bu)影響結(jie)果(guo)。3． 各(ge)(ge)步(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)均(jun)(jun)應(ying)(ying)(ying)(ying)使用(yong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)器(qi)，并經(jing)常校對(dui)(dui)其準(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)，以(yi)(yi)(yi)避(bi)免試(shi)(shi)(shi)(shi)驗(yan)誤(wu)(wu)差(cha)(cha)。一(yi)(yi)次(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)時間＊好(hao)控制在(zai)(zai)5分(fen)(fen)(fen)鐘內(nei)(nei)(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)數(shu)(shu)量(liang)多，推薦(jian)使用(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)。4． 請(qing)(qing)每(mei)次(ci)(ci)(ci)(ci)測(ce)定(ding)(ding)的(de)(de)(de)(de)同(tong)時做(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)，＊好(hao)做(zuo)(zuo)復(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)待(dai)測(ce)物(wu)質(zhi)含量(liang)過高(gao)（樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)OD值(zhi)大于標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)OD值(zhi)），請(qing)(qing)先(xian)用(yong)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)釋(shi)(shi)(shi)(shi)一(yi)(yi)定(ding)(ding)倍(bei)(bei)數(shu)(shu)（n倍(bei)(bei)）后(hou)(hou)(hou)(hou)再(zai)(zai)(zai)(zai)測(ce)定(ding)(ding)，計算(suan)(suan)時請(qing)(qing)＊后(hou)(hou)(hou)(hou)乘以(yi)(yi)(yi)總稀(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)（×n×5）。5． 封(feng)(feng)(feng)板(ban)膜只(zhi)限一(yi)(yi)次(ci)(ci)(ci)(ci)性(xing)(xing)使用(yong)，以(yi)(yi)(yi)避(bi)免交(jiao)叉污(wu)染(ran)。6． 底(di)物(wu)請(qing)(qing)避(bi)光(guang)保(bao)(bao)存。7． 嚴格按(an)照說(shuo)明(ming)書(shu)的(de)(de)(de)(de)操(cao)作(zuo)(zuo)進行，試(shi)(shi)(shi)(shi)驗(yan)結(jie)果(guo)判定(ding)(ding)必(bi)須(xu)以(yi)(yi)(yi)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀數(shu)(shu)為(wei)(wei)準(zhun)(zhun)(zhun)(zhun).8． 所有(you)(you)(you)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)，洗(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)和(he)各(ge)(ge)種廢棄物(wu)都應(ying)(ying)(ying)(ying)按(an)傳染(ran)物(wu)處理。9． 本(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)不(bu)同(tong)批(pi)號(hao)組分(fen)(fen)(fen)不(bu)得(de)混(hun)(hun)(hun)用(yong)。10. 如(ru)與(yu)(yu)(yu)英文(wen)說(shuo)明(ming)書(shu)有(you)(you)(you)異，以(yi)(yi)(yi)英文(wen)說(shuo)明(ming)書(shu)為(wei)(wei)準(zhun)(zhun)(zhun)(zhun)。【計算(suan)(suan)】 以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)的(de)(de)(de)(de)濃度(du)(du)(du)(du)(du)為(wei)(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)為(wei)(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)，根據樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)OD 值(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)查出(chu)相應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)濃度(du)(du)(du)(du)(du)；再(zai)(zai)(zai)(zai)乘以(yi)(yi)(yi)稀(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)數(shu)(shu)；或(huo)用(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)的(de)(de)(de)(de)濃度(du)(du)(du)(du)(du)與(yu)(yu)(yu)OD值(zhi)計算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)的(de)(de)(de)(de)直線(xian)(xian)回歸方(fang)程(cheng)式，將樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)OD值(zhi) 代入方(fang)程(cheng)式，計算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃度(du)(du)(du)(du)(du)，再(zai)(zai)(zai)(zai)乘以(yi)(yi)(yi)稀(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)數(shu)(shu)，即為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)實際濃度(du)(du)(du)(du)(du)。 【試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)(he)組成】試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)(he)組成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)(zhi)保(bao)(bao)存說(shuo)明(ming)書(shu)1份(fen)1份(fen)密(mi)封(feng)(feng)(feng)袋(dai)(dai)1個(ge)1個(ge)封(feng)(feng)(feng)板(ban)膜12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)2片（96）2-8℃保(bao)(bao)存微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)2-8℃保(bao)(bao)存標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)存標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)1.5ml×1瓶(ping)2-8℃保(bao)(bao)存酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)(bao)存樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)(bao)存顯(xian)(xian)(xian)色(se)(se)(se)劑(ji)(ji)A液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)(bao)存顯(xian)(xian)(xian)色(se)(se)(se)劑(ji)(ji)B液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)(bao)存終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)3ml×1瓶(ping)6ml×1瓶(ping)2-8℃保(bao)(bao)存注意：使用(yong)前請(qing)(qing)檢(jian)(jian)查試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)劑(ji)(ji)的(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和(he)數(shu)(shu)量(liang)與(yu)(yu)(yu)表格是(shi)否一(yi)(yi)致。【精(jing)密(mi)度(du)(du)(du)(du)(du)】精(jing)密(mi)度(du)(du)(du)(du)(du)用(yong)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)測(ce)定(ding)(ding)值(zhi)的(de)(de)(de)(de)變異系數(shu)(shu)CV 表示。CV（%） = SD/mean×100批(pi)內(nei)(nei)(nei)差(cha)(cha)：取(qu)(qu)(qu)同(tong)批(pi)次(ci)(ci)(ci)(ci)試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)(he)對(dui)(dui)低(di)、中(zhong)(zhong)(zhong)(zhong)(zhong)、高(gao)值(zhi)定(ding)(ding)值(zhi)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)進行定(ding)(ding)量(liang)檢(jian)(jian)測(ce)，每(mei)份(fen)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)連續測(ce)定(ding)(ding)20 次(ci)(ci)(ci)(ci)，分(fen)(fen)(fen)別(bie)計算(suan)(suan)不(bu)同(tong)濃度(du)(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)(de)(de)平均(jun)(jun)值(zhi)及SD 值(zhi)。批(pi)內(nei)(nei)(nei)差(cha)(cha)： CV<9%批(pi)間差(cha)(cha)：選(xuan)取(qu)(qu)(qu)3 個(ge)不(bu)同(tong)批(pi)次(ci)(ci)(ci)(ci)的(de)(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)別(bie)對(dui)(dui)低(di)、中(zhong)(zhong)(zhong)(zhong)(zhong)、高(gao)值(zhi)定(ding)(ding)值(zhi)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)進行定(ding)(ding)量(liang)測(ce)定(ding)(ding)，每(mei)個(ge)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)使用(yong)同(tong)一(yi)(yi)試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)(he)重復(fu)測(ce)定(ding)(ding)10 次(ci)(ci)(ci)(ci)，分(fen)(fen)(fen)別(bie)計算(suan)(suan)不(bu)同(tong)濃度(du)(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)(de)(de)平均(jun)(jun)值(zhi)及SD 值(zhi)。批(pi)間差(cha)(cha)： CV<11% 【特異性(xing)(xing)】本(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)(he)識別(bie)天(tian)然(ran)和(he)重組微生物(wu)硝酸鹽還原酶(mei)(mei)(mei)(mei)（Nar），經(jing)檢(jian)(jian)測(ce)與(yu)(yu)(yu)其它相似(si)物(wu)質(zhi)無明(ming)顯(xian)(xian)(xian)交(jiao)叉反(fan)應(ying)(ying)(ying)(ying)。由(you)于受到(dao)(dao)技術及樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)來(lai)源的(de)(de)(de)(de)限制，不(bu)可(ke)能完成所有(you)(you)(you)相關或(huo)相似(si)物(wu)質(zhi)交(jiao)叉反(fan)應(ying)(ying)(ying)(ying)檢(jian)(jian)測(ce)，因此本(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)(he)有(you)(you)(you)可(ke)能與(yu)(yu)(yu)未經(jing)檢(jian)(jian)測(ce)的(de)(de)(de)(de)其它物(wu)質(zhi)有(you)(you)(you)交(jiao)叉反(fan)應(ying)(ying)(ying)(ying)。 【穩定(ding)(ding)性(xing)(xing)】試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)(he)在(zai)(zai)有(you)(you)(you)效(xiao)期內(nei)(nei)(nei)務(wu)必(bi)按(an)推薦(jian)溫(wen)(wen)度(du)(du)(du)(du)(du)保(bao)(bao)存，活(huo)性(xing)(xing)降低(di)率(lv)小于5%。為(wei)(wei)減小外部(bu)(bu)因素對(dui)(dui)試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)(he)破(po)壞前后(hou)(hou)(hou)(hou)檢(jian)(jian)測(ce)值(zhi)的(de)(de)(de)(de)影響，實驗(yan)室(shi)的(de)(de)(de)(de)環(huan)境條(tiao)件需盡量(liang)保(bao)(bao)持一(yi)(yi)致，尤其是(shi)實驗(yan)室(shi)內(nei)(nei)(nei)溫(wen)(wen)度(du)(du)(du)(du)(du)、濕度(du)(du)(du)(du)(du)及溫(wen)(wen)育(yu)條(tiao)件。其次(ci)(ci)(ci)(ci)由(you)同(tong)一(yi)(yi)實驗(yan)員來(lai)進行操(cao)作(zuo)(zuo)可(ke)減少(shao)人為(wei)(wei)誤(wu)(wu)差(cha)(cha)。
牛12脂加(jia)(jia)(jia)氧(yang)酶(mei)(12-LO)ELISA 試(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)文(wen)名稱牛12脂加(jia)(jia)(jia)氧(yang)酶(mei)(12-LO)ELISA 試(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)規格(ge)96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)類(lei)型血(xue)(xue)清(qing)、血(xue)(xue)漿、細胞(bao)上(shang)(shang)(shang)清(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)(ye)(ye)(ye)、體(ti)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)、灌洗液(ye)(ye)(ye)(ye)(ye)(ye)(ye)等使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)范圍科研實(shi)驗，不(bu)得(de)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)臨床診斷反(fan)應(ying)(ying)(ying)時間1-5h試(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)保(bao)(bao)(bao)(bao)存(cun)(cun)2-8℃有(you)效期(qi)6個(ge)(ge)月貨(huo)期(qi)3-5天(tian)【操(cao)(cao)(cao)作(zuo)(zuo)步驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)的(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)與(yu)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被板(ban)(ban)(ban)上(shang)(shang)(shang)設標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)第(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別加(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)100μl，然后(hou)(hou)(hou)在(zai)第(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)；然后(hou)(hou)(hou)從第(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)取(qu)(qu)(qu)100μl分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別加(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)第(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)(zai)在(zai)第(di)(di)(di)(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別加(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)；然后(hou)(hou)(hou)在(zai)第(di)(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)第(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)先(xian)(xian)各(ge)取(qu)(qu)(qu)50μl棄(qi)掉(diao)(diao)，再(zai)(zai)(zai)各(ge)取(qu)(qu)(qu)50μl分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別加(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)在(zai)第(di)(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別加(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)50ul，混(hun)(hun)勻(yun)；混(hun)(hun)勻(yun)后(hou)(hou)(hou)從第(di)(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)取(qu)(qu)(qu)50μl分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別加(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)在(zai)第(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別加(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)后(hou)(hou)(hou)從第(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別取(qu)(qu)(qu)50μl加(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)(di)(di)(di)(di)九、第(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)在(zai)第(di)(di)(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別加(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)后(hou)(hou)(hou)從第(di)(di)(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)取(qu)(qu)(qu)50μl棄(qi)掉(diao)(diao)。（稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)都為(wei)(wei)(wei)50μl，濃(nong)(nong)度(du)(du)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別為(wei)(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別設空(kong)(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)(kong)白對(dui)(dui)照孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)及(ji)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)劑(ji)(ji)(ji)，其(qi)余各(ge)步操(cao)(cao)(cao)作(zuo)(zuo)相(xiang)(xiang)同(tong)(tong)）、待測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被板(ban)(ban)(ban)上(shang)(shang)(shang)待測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)先(xian)(xian)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)40μl，然后(hou)(hou)(hou)再(zai)(zai)(zai)加(jia)(jia)(jia)待測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)＊終(zhong)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)度(du)(du)為(wei)(wei)(wei)5倍(bei)(bei)）。加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)加(jia)(jia)(jia)于(yu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底(di)部(bu)，盡(jin)(jin)量(liang)不(bu)觸及(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁(bi)，輕(qing)輕(qing)晃(huang)動(dong)混(hun)(hun)勻(yun)。3. 溫(wen)(wen)育：用(yong)(yong)(yong)(yong)(yong)(yong)封(feng)板(ban)(ban)(ban)膜(mo)(mo)封(feng)板(ban)(ban)(ban)后(hou)(hou)(hou)置(zhi)37℃溫(wen)(wen)育30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘。4. 配液(ye)(ye)(ye)(ye)(ye)(ye)(ye)：將30（48T的(de)20倍(bei)(bei)）倍(bei)(bei)濃(nong)(nong)縮洗滌(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)用(yong)(yong)(yong)(yong)(yong)(yong)蒸(zheng)餾水30（48T的(de)20倍(bei)(bei)）倍(bei)(bei)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)備用(yong)(yong)(yong)(yong)(yong)(yong)。5. 洗滌(di)：小(xiao)心揭掉(diao)(diao)封(feng)板(ban)(ban)(ban)膜(mo)(mo)，棄(qi)去液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，甩干(gan)，每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)滿洗滌(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)，靜置(zhi)30秒后(hou)(hou)(hou)棄(qi)去，如(ru)此(ci)重復5次(ci)(ci)(ci)，拍干(gan)。6. 加(jia)(jia)(jia)酶(mei)：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)入(ru)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)劑(ji)(ji)(ji)50μl，空(kong)(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除(chu)外(wai)。7. 溫(wen)(wen)育：操(cao)(cao)(cao)作(zuo)(zuo)同(tong)(tong)3。8. 洗滌(di)：操(cao)(cao)(cao)作(zuo)(zuo)同(tong)(tong)5。9. 顯(xian)(xian)(xian)色(se)：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)(xian)加(jia)(jia)(jia)入(ru)顯(xian)(xian)(xian)色(se)劑(ji)(ji)(ji)A50μl，再(zai)(zai)(zai)加(jia)(jia)(jia)入(ru)顯(xian)(xian)(xian)色(se)劑(ji)(ji)(ji)B50μl，輕(qing)輕(qing)震蕩混(hun)(hun)勻(yun)，37℃避(bi)光(guang)顯(xian)(xian)(xian)色(se)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘. 10. 終(zhong)止(zhi)(zhi)(zhi)：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)終(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止(zhi)(zhi)(zhi)反(fan)應(ying)(ying)(ying)（此(ci)時藍(lan)色(se)立轉黃色(se)）。11. 測(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)：以(yi)(yi)空(kong)(kong)白空(kong)(kong)調零，450nm波長(chang)依序(xu)測(ce)(ce)量(liang)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)吸光(guang)度(du)(du)（OD值(zhi)(zhi)(zhi)）。 測(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)應(ying)(ying)(ying)在(zai)加(jia)(jia)(jia)終(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘以(yi)(yi)內(nei)進(jin)(jin)行(xing)(xing)(xing)。牛12脂加(jia)(jia)(jia)氧(yang)酶(mei)(12-LO)ELISA 試(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)【注意(yi)(yi)事項】1． 試(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)從冷藏環(huan)境中(zhong)(zhong)(zhong)(zhong)取(qu)(qu)(qu)出(chu)(chu)應(ying)(ying)(ying)在(zai)室溫(wen)(wen)平(ping)衡15-30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘后(hou)(hou)(hou)方可(ke)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)，酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被板(ban)(ban)(ban)開(kai)封(feng)后(hou)(hou)(hou)如(ru)未用(yong)(yong)(yong)(yong)(yong)(yong)完，板(ban)(ban)(ban)條應(ying)(ying)(ying)裝(zhuang)入(ru)密封(feng)袋中(zhong)(zhong)(zhong)(zhong)保(bao)(bao)(bao)(bao)存(cun)(cun)。2． 濃(nong)(nong)洗滌(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)可(ke)能會有(you)結晶析出(chu)(chu)，稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)時可(ke)在(zai)水浴中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)溫(wen)(wen)助溶，洗滌(di)時不(bu)影(ying)響結果。3． 各(ge)步加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)均(jun)應(ying)(ying)(ying)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)器，并經常校對(dui)(dui)其(qi)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)，以(yi)(yi)避(bi)免(mian)試(shi)驗誤差(cha)(cha)(cha)(cha)。一(yi)(yi)次(ci)(ci)(ci)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時間＊好控制在(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘內(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)(shu)(shu)(shu)量(liang)多(duo)，推(tui)薦(jian)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)排(pai)槍加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4． 請(qing)每(mei)(mei)(mei)次(ci)(ci)(ci)測(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)的(de)同(tong)(tong)時做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線，＊好做(zuo)復孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)待測(ce)(ce)物(wu)(wu)(wu)質(zhi)含量(liang)過高（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)OD值(zhi)(zhi)(zhi)大于(yu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)OD值(zhi)(zhi)(zhi)），請(qing)先(xian)(xian)用(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)定(ding)(ding)(ding)(ding)(ding)(ding)倍(bei)(bei)數(shu)(shu)(shu)(shu)（n倍(bei)(bei)）后(hou)(hou)(hou)再(zai)(zai)(zai)測(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)，計(ji)算(suan)時請(qing)＊后(hou)(hou)(hou)乘以(yi)(yi)總稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)（×n×5）。5． 封(feng)板(ban)(ban)(ban)膜(mo)(mo)只限一(yi)(yi)次(ci)(ci)(ci)性(xing)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)，以(yi)(yi)避(bi)免(mian)交叉污(wu)染。6． 底(di)物(wu)(wu)(wu)請(qing)避(bi)光(guang)保(bao)(bao)(bao)(bao)存(cun)(cun)。7． 嚴格(ge)按照說(shuo)明(ming)(ming)書的(de)操(cao)(cao)(cao)作(zuo)(zuo)進(jin)(jin)行(xing)(xing)(xing)，試(shi)驗結果判定(ding)(ding)(ding)(ding)(ding)(ding)必須以(yi)(yi)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀(du)數(shu)(shu)(shu)(shu)為(wei)(wei)(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun).8． 所有(you)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)，洗滌(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)和(he)各(ge)種廢棄(qi)物(wu)(wu)(wu)都應(ying)(ying)(ying)按傳染物(wu)(wu)(wu)處理。9． 本(ben)(ben)(ben)(ben)試(shi)劑(ji)(ji)(ji)不(bu)同(tong)(tong)批(pi)(pi)號組分(fen)(fen)(fen)(fen)(fen)(fen)(fen)不(bu)得(de)混(hun)(hun)用(yong)(yong)(yong)(yong)(yong)(yong)。10. 如(ru)與(yu)英文(wen)說(shuo)明(ming)(ming)書有(you)異，以(yi)(yi)英文(wen)說(shuo)明(ming)(ming)書為(wei)(wei)(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)。【計(ji)算(suan)】 以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)濃(nong)(nong)度(du)(du)為(wei)(wei)(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)繪出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線，根據樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)OD 值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線查出(chu)(chu)相(xiang)(xiang)應(ying)(ying)(ying)的(de)濃(nong)(nong)度(du)(du)；再(zai)(zai)(zai)乘以(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi) 倍(bei)(bei)數(shu)(shu)(shu)(shu)；或用(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)濃(nong)(nong)度(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)算(suan)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線的(de)直線回歸方程式(shi)，將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)OD值(zhi)(zhi)(zhi) 代(dai)入(ru)方程式(shi)，計(ji)算(suan)出(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)，再(zai)(zai)(zai)乘以(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi) 倍(bei)(bei)數(shu)(shu)(shu)(shu)，即為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)實(shi)際濃(nong)(nong)度(du)(du)。 【試(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)組成】試(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)組成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)保(bao)(bao)(bao)(bao)存(cun)(cun)說(shuo)明(ming)(ming)書1份(fen)1份(fen)密封(feng)袋1個(ge)(ge)1個(ge)(ge)封(feng)板(ban)(ban)(ban)膜(mo)(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條2片（96）2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)劑(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)顯(xian)(xian)(xian)色(se)劑(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)顯(xian)(xian)(xian)色(se)劑(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)終(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)注意(yi)(yi)：使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)前請(qing)檢查試(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)試(shi)劑(ji)(ji)(ji)的(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和(he)數(shu)(shu)(shu)(shu)量(liang)與(yu)表格(ge)是(shi)否一(yi)(yi)致。【精密度(du)(du)】精密度(du)(du)用(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)測(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)的(de)變異系數(shu)(shu)(shu)(shu)CV 表示。CV（%） = SD/mean×100批(pi)(pi)內(nei)差(cha)(cha)(cha)(cha)：取(qu)(qu)(qu)同(tong)(tong)批(pi)(pi)次(ci)(ci)(ci)試(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)對(dui)(dui)低、中(zhong)(zhong)(zhong)(zhong)、高值(zhi)(zhi)(zhi)定(ding)(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)進(jin)(jin)行(xing)(xing)(xing)定(ding)(ding)(ding)(ding)(ding)(ding)量(liang)檢測(ce)(ce)，每(mei)(mei)(mei)份(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)連(lian)續測(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)20 次(ci)(ci)(ci)，分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別計(ji)算(suan)不(bu)同(tong)(tong)濃(nong)(nong)度(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)平(ping)均(jun)值(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)。批(pi)(pi)內(nei)差(cha)(cha)(cha)(cha)： CV<9%批(pi)(pi)間差(cha)(cha)(cha)(cha)：選取(qu)(qu)(qu)3 個(ge)(ge)不(bu)同(tong)(tong)批(pi)(pi)次(ci)(ci)(ci)的(de)試(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別對(dui)(dui)低、中(zhong)(zhong)(zhong)(zhong)、高值(zhi)(zhi)(zhi)定(ding)(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)進(jin)(jin)行(xing)(xing)(xing)定(ding)(ding)(ding)(ding)(ding)(ding)量(liang)測(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)，每(mei)(mei)(mei)個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)同(tong)(tong)一(yi)(yi)試(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)重復測(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)10 次(ci)(ci)(ci)，分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別計(ji)算(suan)不(bu)同(tong)(tong)濃(nong)(nong)度(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)平(ping)均(jun)值(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)。批(pi)(pi)間差(cha)(cha)(cha)(cha)： CV<11% 【特異性(xing)】本(ben)(ben)(ben)(ben)試(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)識別天(tian)然和(he)重組微生物(wu)(wu)(wu)硝(xiao)酸鹽(yan)還原酶(mei)（Nar），經檢測(ce)(ce)與(yu)其(qi)它(ta)(ta)相(xiang)(xiang)似物(wu)(wu)(wu)質(zhi)無明(ming)(ming)顯(xian)(xian)(xian)交叉反(fan)應(ying)(ying)(ying)。由于(yu)受(shou)到(dao)(dao)技(ji)術及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)來源的(de)限制，不(bu)可(ke)能完成所有(you)相(xiang)(xiang)關或相(xiang)(xiang)似物(wu)(wu)(wu)質(zhi)交叉反(fan)應(ying)(ying)(ying)檢測(ce)(ce)，因(yin)此(ci)本(ben)(ben)(ben)(ben)試(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)有(you)可(ke)能與(yu)未經檢測(ce)(ce)的(de)其(qi)它(ta)(ta)物(wu)(wu)(wu)質(zhi)有(you)交叉反(fan)應(ying)(ying)(ying)。 【穩定(ding)(ding)(ding)(ding)(ding)(ding)性(xing)】試(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)在(zai)有(you)效期(qi)內(nei)務必按推(tui)薦(jian)溫(wen)(wen)度(du)(du)保(bao)(bao)(bao)(bao)存(cun)(cun)，活性(xing)降低率小(xiao)于(yu)5%。為(wei)(wei)(wei)減(jian)小(xiao)外(wai)部(bu)因(yin)素對(dui)(dui)試(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)破壞前后(hou)(hou)(hou)檢測(ce)(ce)值(zhi)(zhi)(zhi)的(de)影(ying)響，實(shi)驗室的(de)環(huan)境條件需盡(jin)(jin)量(liang)保(bao)(bao)(bao)(bao)持一(yi)(yi)致，尤其(qi)是(shi)實(shi)驗室內(nei)溫(wen)(wen)度(du)(du)、濕度(du)(du)及(ji)溫(wen)(wen)育條件。其(qi)次(ci)(ci)(ci)由同(tong)(tong)一(yi)(yi)實(shi)驗員(yuan)來進(jin)(jin)行(xing)(xing)(xing)操(cao)(cao)(cao)作(zuo)(zuo)可(ke)減(jian)少人為(wei)(wei)(wei)誤差(cha)(cha)(cha)(cha)。
牛(niu)可(ke)(ke)(ke)(ke)溶性(xing)胸苷激(ji)酶(mei)(mei)1(TK1)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)文名(ming)稱牛(niu)可(ke)(ke)(ke)(ke)溶性(xing)胸苷激(ji)酶(mei)(mei)1(TK1)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)規格(ge)(ge)96T/48T樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)類型血(xue)清、血(xue)漿、細胞上(shang)(shang)清液(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)、體液(ye)(ye)(ye)(ye)、灌洗(xi)液(ye)(ye)(ye)(ye)等使(shi)用(yong)(yong)(yong)(yong)范(fan)圍科研實(shi)(shi)驗(yan)(yan)，不(bu)(bu)(bu)得(de)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)臨(lin)床診(zhen)斷反(fan)應時(shi)(shi)間(jian)(jian)1-5h試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)保(bao)存(cun)(cun)(cun)(cun)2-8℃有(you)(you)效(xiao)期6個(ge)月貨(huo)期3-5天【操(cao)作(zuo)(zuo)步(bu)驟】1. 標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)與(yu)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)：在酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)上(shang)(shang)設標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在第(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，然(ran)后(hou)(hou)(hou)(hou)在第(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)；然(ran)后(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各取(qu)(qu)(qu)100μl分(fen)(fen)(fen)別加(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)第(di)(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)在第(di)(di)(di)(di)(di)(di)(di)(di)三、第(di)(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)；然(ran)后(hou)(hou)(hou)(hou)在第(di)(di)(di)(di)(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)第(di)(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)先(xian)(xian)各取(qu)(qu)(qu)50μl棄(qi)掉，再(zai)(zai)各取(qu)(qu)(qu)50μl分(fen)(fen)(fen)別加(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在第(di)(di)(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)50ul，混(hun)(hun)勻(yun)；混(hun)(hun)勻(yun)后(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各取(qu)(qu)(qu)50μl分(fen)(fen)(fen)別加(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在第(di)(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)后(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)別取(qu)(qu)(qu)50μl加(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)(di)九(jiu)(jiu)(jiu)、第(di)(di)(di)(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在第(di)(di)(di)(di)(di)(di)(di)(di)九(jiu)(jiu)(jiu)第(di)(di)(di)(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻(yun)后(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)九(jiu)(jiu)(jiu)第(di)(di)(di)(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各取(qu)(qu)(qu)50μl棄(qi)掉。（稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)量(liang)(liang)都為(wei)50μl，濃(nong)(nong)(nong)(nong)度(du)(du)(du)分(fen)(fen)(fen)別為(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)：分(fen)(fen)(fen)別設空(kong)(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)(kong)白(bai)對照(zhao)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)及(ji)(ji)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，其(qi)(qi)余(yu)各步(bu)操(cao)作(zuo)(zuo)相(xiang)同(tong)(tong)(tong)）、待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)上(shang)(shang)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)先(xian)(xian)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)40μl，然(ran)后(hou)(hou)(hou)(hou)再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)＊終稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)度(du)(du)(du)為(wei)5倍(bei)(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)將樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)于(yu)(yu)(yu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部(bu)，盡量(liang)(liang)不(bu)(bu)(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)輕(qing)晃動混(hun)(hun)勻(yun)。3. 溫(wen)育(yu)：用(yong)(yong)(yong)(yong)封(feng)(feng)板(ban)(ban)膜(mo)封(feng)(feng)板(ban)(ban)后(hou)(hou)(hou)(hou)置(zhi)37℃溫(wen)育(yu)30分(fen)(fen)(fen)鐘。4. 配液(ye)(ye)(ye)(ye)：將30（48T的(de)(de)(de)(de)(de)20倍(bei)(bei)(bei)）倍(bei)(bei)(bei)濃(nong)(nong)(nong)(nong)縮(suo)洗(xi)滌(di)(di)液(ye)(ye)(ye)(ye)用(yong)(yong)(yong)(yong)蒸餾水(shui)30（48T的(de)(de)(de)(de)(de)20倍(bei)(bei)(bei)）倍(bei)(bei)(bei)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)備用(yong)(yong)(yong)(yong)。5. 洗(xi)滌(di)(di)：小(xiao)(xiao)心揭掉封(feng)(feng)板(ban)(ban)膜(mo)，棄(qi)去(qu)(qu)液(ye)(ye)(ye)(ye)體，甩干，每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)滿(man)洗(xi)滌(di)(di)液(ye)(ye)(ye)(ye)，靜置(zhi)30秒后(hou)(hou)(hou)(hou)棄(qi)去(qu)(qu)，如(ru)(ru)(ru)此(ci)重(zhong)復5次(ci)(ci)，拍干。6. 加(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)入(ru)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)50μl，空(kong)(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外。7. 溫(wen)育(yu)：操(cao)作(zuo)(zuo)同(tong)(tong)(tong)3。8. 洗(xi)滌(di)(di)：操(cao)作(zuo)(zuo)同(tong)(tong)(tong)5。9. 顯(xian)(xian)(xian)色(se)：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)(xian)加(jia)(jia)(jia)(jia)(jia)入(ru)顯(xian)(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)A50μl，再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)入(ru)顯(xian)(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)B50μl，輕(qing)輕(qing)震蕩混(hun)(hun)勻(yun)，37℃避光顯(xian)(xian)(xian)色(se)15分(fen)(fen)(fen)鐘. 10. 終止：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)終止液(ye)(ye)(ye)(ye)50μl，終止反(fan)應（此(ci)時(shi)(shi)藍色(se)立(li)轉黃色(se)）。11. 測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)：以空(kong)(kong)白(bai)空(kong)(kong)調(diao)零，450nm波長依序測(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)吸光度(du)(du)(du)（OD值(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)應在加(jia)(jia)(jia)(jia)(jia)終止液(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)鐘以內(nei)進(jin)行(xing)。牛(niu)可(ke)(ke)(ke)(ke)溶性(xing)胸苷激(ji)酶(mei)(mei)1(TK1)ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)【注意(yi)(yi)事(shi)項】1． 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)從(cong)冷藏環(huan)境中(zhong)(zhong)(zhong)(zhong)(zhong)取(qu)(qu)(qu)出(chu)(chu)應在室溫(wen)平衡15-30分(fen)(fen)(fen)鐘后(hou)(hou)(hou)(hou)方(fang)可(ke)(ke)(ke)(ke)使(shi)用(yong)(yong)(yong)(yong)，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)開封(feng)(feng)后(hou)(hou)(hou)(hou)如(ru)(ru)(ru)未用(yong)(yong)(yong)(yong)完(wan)，板(ban)(ban)條(tiao)(tiao)應裝入(ru)密(mi)封(feng)(feng)袋中(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)存(cun)(cun)(cun)(cun)。2． 濃(nong)(nong)(nong)(nong)洗(xi)滌(di)(di)液(ye)(ye)(ye)(ye)可(ke)(ke)(ke)(ke)能會有(you)(you)結(jie)晶析出(chu)(chu)，稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)時(shi)(shi)可(ke)(ke)(ke)(ke)在水(shui)浴中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)溫(wen)助(zhu)溶，洗(xi)滌(di)(di)時(shi)(shi)不(bu)(bu)(bu)影響結(jie)果。3． 各步(bu)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)均(jun)應使(shi)用(yong)(yong)(yong)(yong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)器(qi)，并經(jing)常校對其(qi)(qi)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)，以避免(mian)試(shi)(shi)(shi)(shi)驗(yan)(yan)誤差(cha)。一(yi)(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)時(shi)(shi)間(jian)(jian)＊好控制在5分(fen)(fen)(fen)鐘內(nei)，如(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)數(shu)量(liang)(liang)多，推薦(jian)使(shi)用(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)。4． 請每(mei)(mei)(mei)次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)的(de)(de)(de)(de)(de)同(tong)(tong)(tong)時(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)，＊好做復孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)質(zhi)含量(liang)(liang)過高（樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)OD值(zhi)(zhi)(zhi)大于(yu)(yu)(yu)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)），請先(xian)(xian)用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)一(yi)(yi)定(ding)(ding)(ding)倍(bei)(bei)(bei)數(shu)（n倍(bei)(bei)(bei)）后(hou)(hou)(hou)(hou)再(zai)(zai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，計算(suan)(suan)(suan)時(shi)(shi)請＊后(hou)(hou)(hou)(hou)乘(cheng)以總稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)（×n×5）。5． 封(feng)(feng)板(ban)(ban)膜(mo)只限(xian)一(yi)(yi)次(ci)(ci)性(xing)使(shi)用(yong)(yong)(yong)(yong)，以避免(mian)交叉(cha)(cha)污(wu)染。6． 底物(wu)(wu)請避光保(bao)存(cun)(cun)(cun)(cun)。7． 嚴格(ge)(ge)按(an)(an)(an)照(zhao)說(shuo)(shuo)明(ming)書的(de)(de)(de)(de)(de)操(cao)作(zuo)(zuo)進(jin)行(xing)，試(shi)(shi)(shi)(shi)驗(yan)(yan)結(jie)果判定(ding)(ding)(ding)必(bi)須以酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)儀讀數(shu)為(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun).8． 所(suo)有(you)(you)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)，洗(xi)滌(di)(di)液(ye)(ye)(ye)(ye)和(he)(he)各種廢棄(qi)物(wu)(wu)都應按(an)(an)(an)傳染物(wu)(wu)處(chu)理。9． 本(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)不(bu)(bu)(bu)同(tong)(tong)(tong)批(pi)(pi)號組(zu)分(fen)(fen)(fen)不(bu)(bu)(bu)得(de)混(hun)(hun)用(yong)(yong)(yong)(yong)。10. 如(ru)(ru)(ru)與(yu)英文說(shuo)(shuo)明(ming)書有(you)(you)異(yi)，以英文說(shuo)(shuo)明(ming)書為(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)。【計算(suan)(suan)(suan)】 以標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)， 在坐(zuo)標(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)繪出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)，根據(ju)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)OD 值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)查出(chu)(chu)相(xiang)應的(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)；再(zai)(zai)乘(cheng)以稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)(bei)數(shu)；或(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計算(suan)(suan)(suan)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)的(de)(de)(de)(de)(de)直(zhi)線(xian)回歸方(fang)程(cheng)式，將樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi) 代入(ru)方(fang)程(cheng)式，計算(suan)(suan)(suan)出(chu)(chu)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)，再(zai)(zai)乘(cheng)以稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)(bei)數(shu)，即為(wei)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)實(shi)(shi)際濃(nong)(nong)(nong)(nong)度(du)(du)(du)。 【試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)組(zu)成】試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)組(zu)成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)保(bao)存(cun)(cun)(cun)(cun)說(shuo)(shuo)明(ming)書1份(fen)1份(fen)密(mi)封(feng)(feng)袋1個(ge)1個(ge)封(feng)(feng)板(ban)(ban)膜(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)(tiao)2片（96）2-8℃保(bao)存(cun)(cun)(cun)(cun)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)板(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)(tiao)2-8℃保(bao)存(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)存(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)顯(xian)(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)顯(xian)(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)終止液(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)注意(yi)(yi)：使(shi)用(yong)(yong)(yong)(yong)前請檢查試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)簽和(he)(he)數(shu)量(liang)(liang)與(yu)表(biao)格(ge)(ge)是(shi)(shi)否一(yi)(yi)致(zhi)。【精(jing)密(mi)度(du)(du)(du)】精(jing)密(mi)度(du)(du)(du)用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)變異(yi)系數(shu)CV 表(biao)示。CV（%） = SD/mean×100批(pi)(pi)內(nei)差(cha)：取(qu)(qu)(qu)同(tong)(tong)(tong)批(pi)(pi)次(ci)(ci)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)對低、中(zhong)(zhong)(zhong)(zhong)(zhong)、高值(zhi)(zhi)(zhi)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)進(jin)行(xing)定(ding)(ding)(ding)量(liang)(liang)檢測(ce)(ce)(ce)(ce)(ce)(ce)(ce)，每(mei)(mei)(mei)份(fen)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)連續測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)20 次(ci)(ci)，分(fen)(fen)(fen)別計算(suan)(suan)(suan)不(bu)(bu)(bu)同(tong)(tong)(tong)濃(nong)(nong)(nong)(nong)度(du)(du)(du)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)平均(jun)值(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)。批(pi)(pi)內(nei)差(cha)： CV<9%批(pi)(pi)間(jian)(jian)差(cha)：選取(qu)(qu)(qu)3 個(ge)不(bu)(bu)(bu)同(tong)(tong)(tong)批(pi)(pi)次(ci)(ci)的(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)分(fen)(fen)(fen)別對低、中(zhong)(zhong)(zhong)(zhong)(zhong)、高值(zhi)(zhi)(zhi)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)進(jin)行(xing)定(ding)(ding)(ding)量(liang)(liang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，每(mei)(mei)(mei)個(ge)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)使(shi)用(yong)(yong)(yong)(yong)同(tong)(tong)(tong)一(yi)(yi)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)重(zhong)復測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)10 次(ci)(ci)，分(fen)(fen)(fen)別計算(suan)(suan)(suan)不(bu)(bu)(bu)同(tong)(tong)(tong)濃(nong)(nong)(nong)(nong)度(du)(du)(du)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)平均(jun)值(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)。批(pi)(pi)間(jian)(jian)差(cha)： CV<11% 【特異(yi)性(xing)】本(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)識別天然(ran)和(he)(he)重(zhong)組(zu)微生物(wu)(wu)硝(xiao)酸鹽還原酶(mei)(mei)（Nar），經(jing)檢測(ce)(ce)(ce)(ce)(ce)(ce)(ce)與(yu)其(qi)(qi)它(ta)相(xiang)似物(wu)(wu)質(zhi)無明(ming)顯(xian)(xian)(xian)交叉(cha)(cha)反(fan)應。由于(yu)(yu)(yu)受到(dao)技術及(ji)(ji)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)來(lai)源的(de)(de)(de)(de)(de)限(xian)制，不(bu)(bu)(bu)可(ke)(ke)(ke)(ke)能完(wan)成所(suo)有(you)(you)相(xiang)關或(huo)相(xiang)似物(wu)(wu)質(zhi)交叉(cha)(cha)反(fan)應檢測(ce)(ce)(ce)(ce)(ce)(ce)(ce)，因(yin)此(ci)本(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)有(you)(you)可(ke)(ke)(ke)(ke)能與(yu)未經(jing)檢測(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)其(qi)(qi)它(ta)物(wu)(wu)質(zhi)有(you)(you)交叉(cha)(cha)反(fan)應。 【穩定(ding)(ding)(ding)性(xing)】試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)在有(you)(you)效(xiao)期內(nei)務(wu)必(bi)按(an)(an)(an)推薦(jian)溫(wen)度(du)(du)(du)保(bao)存(cun)(cun)(cun)(cun)，活(huo)性(xing)降低率小(xiao)(xiao)于(yu)(yu)(yu)5%。為(wei)減(jian)小(xiao)(xiao)外部(bu)因(yin)素對試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)破壞前后(hou)(hou)(hou)(hou)檢測(ce)(ce)(ce)(ce)(ce)(ce)(ce)值(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)影響，實(shi)(shi)驗(yan)(yan)室的(de)(de)(de)(de)(de)環(huan)境條(tiao)(tiao)件(jian)需盡量(liang)(liang)保(bao)持一(yi)(yi)致(zhi)，尤其(qi)(qi)是(shi)(shi)實(shi)(shi)驗(yan)(yan)室內(nei)溫(wen)度(du)(du)(du)、濕(shi)度(du)(du)(du)及(ji)(ji)溫(wen)育(yu)條(tiao)(tiao)件(jian)。其(qi)(qi)次(ci)(ci)由同(tong)(tong)(tong)一(yi)(yi)實(shi)(shi)驗(yan)(yan)員來(lai)進(jin)行(xing)操(cao)作(zuo)(zuo)可(ke)(ke)(ke)(ke)減(jian)少人為(wei)誤差(cha)。
牛(niu)(niu)白(bai)介(jie)(jie)素(su)35(IL-35)ELISA 試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)文名稱(cheng)牛(niu)(niu)白(bai)介(jie)(jie)素(su)35(IL-35)ELISA 試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)規格(ge)96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)類(lei)型血清、血漿、細胞上(shang)清液(ye)(ye)(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)(ye)(ye)、體液(ye)(ye)(ye)(ye)(ye)(ye)、灌洗(xi)液(ye)(ye)(ye)(ye)(ye)(ye)等使(shi)(shi)用(yong)(yong)(yong)范圍科(ke)研實(shi)(shi)(shi)驗(yan)，不得(de)用(yong)(yong)(yong)于臨床(chuang)診斷反(fan)應(ying)時(shi)間1-5h試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)2-8℃有(you)效(xiao)(xiao)期(qi)(qi)6個(ge)(ge)(ge)月貨(huo)期(qi)(qi)3-5天【操(cao)作(zuo)步驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)稀(xi)釋(shi)與(yu)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)(zai)(zai)(zai)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)上(shang)設標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，然(ran)后(hou)(hou)(hou)(hou)在(zai)(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)(yun)；然(ran)后(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)100μl分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)第(di)(di)(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)(zai)(zai)在(zai)(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)三、第(di)(di)(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)(yun)；然(ran)后(hou)(hou)(hou)(hou)在(zai)(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)第(di)(di)(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)先各(ge)(ge)取(qu)50μl棄掉，再(zai)(zai)(zai)(zai)各(ge)(ge)取(qu)50μl分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)(zai)在(zai)(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)液(ye)(ye)(ye)(ye)(ye)(ye)50ul，混(hun)勻(yun)(yun)；混(hun)勻(yun)(yun)后(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)50μl分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)(zai)在(zai)(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)(yun)后(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)取(qu)50μl加(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)(di)(di)(di)九、第(di)(di)(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)(zai)(zai)在(zai)(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)(yun)后(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)取(qu)50μl棄掉。（稀(xi)釋(shi)后(hou)(hou)(hou)(hou)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)量都為(wei)(wei)50μl，濃(nong)(nong)(nong)度(du)(du)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)為(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)設空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)（空白(bai)對照孔(kong)(kong)(kong)(kong)(kong)(kong)不加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)及(ji)(ji)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)劑(ji)(ji)(ji)，其(qi)(qi)余各(ge)(ge)步操(cao)作(zuo)相(xiang)同(tong)(tong)(tong)）、待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)(zai)(zai)(zai)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)上(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)先加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)液(ye)(ye)(ye)(ye)(ye)(ye)40μl，然(ran)后(hou)(hou)(hou)(hou)再(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)＊終(zhong)稀(xi)釋(shi)度(du)(du)為(wei)(wei)5倍(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)于酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)底部，盡量不觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)輕(qing)晃動(dong)混(hun)勻(yun)(yun)。3. 溫(wen)(wen)育(yu)：用(yong)(yong)(yong)封板(ban)膜封板(ban)后(hou)(hou)(hou)(hou)置(zhi)37℃溫(wen)(wen)育(yu)30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。4. 配液(ye)(ye)(ye)(ye)(ye)(ye)：將30（48T的(de)(de)(de)20倍(bei)(bei)）倍(bei)(bei)濃(nong)(nong)(nong)縮洗(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)用(yong)(yong)(yong)蒸餾水(shui)30（48T的(de)(de)(de)20倍(bei)(bei)）倍(bei)(bei)稀(xi)釋(shi)后(hou)(hou)(hou)(hou)備(bei)用(yong)(yong)(yong)。5. 洗(xi)滌(di)(di)：小(xiao)心揭(jie)掉封板(ban)膜，棄去(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體，甩干(gan)，每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)滿洗(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)，靜置(zhi)30秒后(hou)(hou)(hou)(hou)棄去(qu)，如(ru)此重(zhong)復(fu)5次，拍干(gan)。6. 加(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)(mei)(mei)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)入(ru)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)劑(ji)(ji)(ji)50μl，空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)除外。7. 溫(wen)(wen)育(yu)：操(cao)作(zuo)同(tong)(tong)(tong)3。8. 洗(xi)滌(di)(di)：操(cao)作(zuo)同(tong)(tong)(tong)5。9. 顯(xian)色：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)(jia)入(ru)顯(xian)色劑(ji)(ji)(ji)A50μl，再(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)入(ru)顯(xian)色劑(ji)(ji)(ji)B50μl，輕(qing)輕(qing)震蕩混(hun)勻(yun)(yun)，37℃避(bi)光(guang)(guang)顯(xian)色15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong). 10. 終(zhong)止：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止反(fan)應(ying)（此時(shi)藍色立轉黃色）。11. 測(ce)(ce)(ce)(ce)(ce)定：以(yi)(yi)空白(bai)空調零，450nm波長依序測(ce)(ce)(ce)(ce)(ce)量各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)吸光(guang)(guang)度(du)(du)（OD值(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)(ce)(ce)定應(ying)在(zai)(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)內進(jin)(jin)行(xing)。牛(niu)(niu)白(bai)介(jie)(jie)素(su)35(IL-35)ELISA 試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)【注意事項】1． 試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)從(cong)冷藏(zang)環境中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)取(qu)出(chu)應(ying)在(zai)(zai)(zai)(zai)(zai)室(shi)(shi)(shi)溫(wen)(wen)平(ping)衡15-30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)方可(ke)(ke)使(shi)(shi)用(yong)(yong)(yong)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)開(kai)封后(hou)(hou)(hou)(hou)如(ru)未用(yong)(yong)(yong)完，板(ban)條(tiao)應(ying)裝入(ru)密(mi)封袋中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)。2． 濃(nong)(nong)(nong)洗(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)可(ke)(ke)能會有(you)結晶析出(chu)，稀(xi)釋(shi)時(shi)可(ke)(ke)在(zai)(zai)(zai)(zai)(zai)水(shui)浴中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)溫(wen)(wen)助溶(rong)，洗(xi)滌(di)(di)時(shi)不影(ying)響(xiang)結果(guo)。3． 各(ge)(ge)步加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)均應(ying)使(shi)(shi)用(yong)(yong)(yong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)器(qi)，并經(jing)常校對其(qi)(qi)準(zhun)(zhun)(zhun)(zhun)確性(xing)，以(yi)(yi)避(bi)免試(shi)(shi)驗(yan)誤差(cha)(cha)。一(yi)(yi)(yi)(yi)次加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)間＊好控制(zhi)在(zai)(zai)(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)數(shu)(shu)(shu)量多，推(tui)薦使(shi)(shi)用(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4． 請每(mei)(mei)次測(ce)(ce)(ce)(ce)(ce)定的(de)(de)(de)同(tong)(tong)(tong)時(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線，＊好做復(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)物(wu)(wu)質(zhi)(zhi)含量過高（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)OD值(zhi)(zhi)(zhi)大于標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)OD值(zhi)(zhi)(zhi)），請先用(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)釋(shi)一(yi)(yi)(yi)(yi)定倍(bei)(bei)數(shu)(shu)(shu)（n倍(bei)(bei)）后(hou)(hou)(hou)(hou)再(zai)(zai)(zai)(zai)測(ce)(ce)(ce)(ce)(ce)定，計(ji)算(suan)(suan)時(shi)請＊后(hou)(hou)(hou)(hou)乘以(yi)(yi)總稀(xi)釋(shi)倍(bei)(bei)數(shu)(shu)(shu)（×n×5）。5． 封板(ban)膜只(zhi)限(xian)一(yi)(yi)(yi)(yi)次性(xing)使(shi)(shi)用(yong)(yong)(yong)，以(yi)(yi)避(bi)免交叉(cha)污染。6． 底物(wu)(wu)請避(bi)光(guang)(guang)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)。7． 嚴格(ge)按(an)(an)照說(shuo)明書(shu)的(de)(de)(de)操(cao)作(zuo)進(jin)(jin)行(xing)，試(shi)(shi)驗(yan)結果(guo)判定必須(xu)以(yi)(yi)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)讀數(shu)(shu)(shu)為(wei)(wei)準(zhun)(zhun)(zhun)(zhun).8． 所有(you)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)，洗(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)和(he)(he)各(ge)(ge)種廢棄物(wu)(wu)都應(ying)按(an)(an)傳(chuan)染物(wu)(wu)處理。9． 本(ben)(ben)試(shi)(shi)劑(ji)(ji)(ji)不同(tong)(tong)(tong)批號組分(fen)(fen)(fen)(fen)不得(de)混(hun)用(yong)(yong)(yong)。10. 如(ru)與(yu)英(ying)文說(shuo)明書(shu)有(you)異(yi)，以(yi)(yi)英(ying)文說(shuo)明書(shu)為(wei)(wei)準(zhun)(zhun)(zhun)(zhun)。【計(ji)算(suan)(suan)】 以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)為(wei)(wei)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)(zai)(zai)(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線，根(gen)據樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)OD 值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線查出(chu)相(xiang)應(ying)的(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)；再(zai)(zai)(zai)(zai)乘以(yi)(yi)稀(xi)釋(shi) 倍(bei)(bei)數(shu)(shu)(shu)；或用(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線的(de)(de)(de)直線回歸(gui)方程式，將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)OD值(zhi)(zhi)(zhi) 代(dai)入(ru)方程式，計(ji)算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)，再(zai)(zai)(zai)(zai)乘以(yi)(yi)稀(xi)釋(shi) 倍(bei)(bei)數(shu)(shu)(shu)，即(ji)為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)實(shi)(shi)(shi)際濃(nong)(nong)(nong)度(du)(du)。 【試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)組成(cheng)】試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)組成(cheng)48孔(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)說(shuo)明書(shu)1份(fen)1份(fen)密(mi)封袋1個(ge)(ge)(ge)1個(ge)(ge)(ge)封板(ban)膜12孔(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)2片（96）2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)液(ye)(ye)(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)劑(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)釋(shi)液(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)顯(xian)色劑(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)顯(xian)色劑(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)注意：使(shi)(shi)用(yong)(yong)(yong)前請檢(jian)查試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)劑(ji)(ji)(ji)的(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和(he)(he)數(shu)(shu)(shu)量與(yu)表(biao)格(ge)是否一(yi)(yi)(yi)(yi)致(zhi)。【精(jing)密(mi)度(du)(du)】精(jing)密(mi)度(du)(du)用(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)定值(zhi)(zhi)(zhi)的(de)(de)(de)變異(yi)系數(shu)(shu)(shu)CV 表(biao)示(shi)。CV（%） = SD/mean×100批內差(cha)(cha)：取(qu)同(tong)(tong)(tong)批次試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)對低(di)、中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)、高值(zhi)(zhi)(zhi)定值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)進(jin)(jin)行(xing)定量檢(jian)測(ce)(ce)(ce)(ce)(ce)，每(mei)(mei)份(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)連(lian)續測(ce)(ce)(ce)(ce)(ce)定20 次，分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)計(ji)算(suan)(suan)不同(tong)(tong)(tong)濃(nong)(nong)(nong)度(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)(de)平(ping)均值(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)。批內差(cha)(cha)： CV<9%批間差(cha)(cha)：選(xuan)取(qu)3 個(ge)(ge)(ge)不同(tong)(tong)(tong)批次的(de)(de)(de)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)對低(di)、中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)、高值(zhi)(zhi)(zhi)定值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)進(jin)(jin)行(xing)定量測(ce)(ce)(ce)(ce)(ce)定，每(mei)(mei)個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)使(shi)(shi)用(yong)(yong)(yong)同(tong)(tong)(tong)一(yi)(yi)(yi)(yi)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)重(zhong)復(fu)測(ce)(ce)(ce)(ce)(ce)定10 次，分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)計(ji)算(suan)(suan)不同(tong)(tong)(tong)濃(nong)(nong)(nong)度(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)(de)平(ping)均值(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)。批間差(cha)(cha)： CV<11% 【特異(yi)性(xing)】本(ben)(ben)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)識別(bie)(bie)(bie)天然(ran)和(he)(he)重(zhong)組微(wei)生物(wu)(wu)硝酸(suan)鹽還原酶(mei)(mei)(mei)(mei)（Nar），經(jing)檢(jian)測(ce)(ce)(ce)(ce)(ce)與(yu)其(qi)(qi)它(ta)相(xiang)似物(wu)(wu)質(zhi)(zhi)無(wu)明顯(xian)交叉(cha)反(fan)應(ying)。由于受到技(ji)術及(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)來源(yuan)的(de)(de)(de)限(xian)制(zhi)，不可(ke)(ke)能完成(cheng)所有(you)相(xiang)關或相(xiang)似物(wu)(wu)質(zhi)(zhi)交叉(cha)反(fan)應(ying)檢(jian)測(ce)(ce)(ce)(ce)(ce)，因此本(ben)(ben)試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)有(you)可(ke)(ke)能與(yu)未經(jing)檢(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)其(qi)(qi)它(ta)物(wu)(wu)質(zhi)(zhi)有(you)交叉(cha)反(fan)應(ying)。 【穩定性(xing)】試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)在(zai)(zai)(zai)(zai)(zai)有(you)效(xiao)(xiao)期(qi)(qi)內務必按(an)(an)推(tui)薦溫(wen)(wen)度(du)(du)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)，活(huo)性(xing)降低(di)率小(xiao)于5%。為(wei)(wei)減小(xiao)外部因素(su)對試(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)破壞前后(hou)(hou)(hou)(hou)檢(jian)測(ce)(ce)(ce)(ce)(ce)值(zhi)(zhi)(zhi)的(de)(de)(de)影(ying)響(xiang)，實(shi)(shi)(shi)驗(yan)室(shi)(shi)(shi)的(de)(de)(de)環境條(tiao)件需盡量保(bao)(bao)(bao)(bao)持一(yi)(yi)(yi)(yi)致(zhi)，尤其(qi)(qi)是實(shi)(shi)(shi)驗(yan)室(shi)(shi)(shi)內溫(wen)(wen)度(du)(du)、濕度(du)(du)及(ji)(ji)溫(wen)(wen)育(yu)條(tiao)件。其(qi)(qi)次由同(tong)(tong)(tong)一(yi)(yi)(yi)(yi)實(shi)(shi)(shi)驗(yan)員來進(jin)(jin)行(xing)操(cao)作(zuo)可(ke)(ke)減少人為(wei)(wei)誤差(cha)(cha)。
牛(niu)垂體腺(xian)苷(gan)酸(suan)環(huan)化(hua)(hua)酶(mei)(mei)激(ji)活肽(tai)27(PACAP-27)ELISA 試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)文名稱牛(niu)垂體腺(xian)苷(gan)酸(suan)環(huan)化(hua)(hua)酶(mei)(mei)激(ji)活肽(tai)27(PACAP-27)ELISA 試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)規格96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)類型(xing)血清、血漿、細胞上清液(ye)、尿(niao)液(ye)、體液(ye)、灌洗(xi)液(ye)等使用(yong)(yong)范圍科研(yan)實驗(yan)(yan)，不(bu)得用(yong)(yong)于(yu)臨床診斷(duan)反(fan)應(ying)(ying)時間1-5h試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)2-8℃有(you)效(xiao)期6個月(yue)貨(huo)期3-5天(tian)(tian)【操(cao)(cao)(cao)作(zuo)(zuo)步驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)與(yu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)(ban)(ban)(ban)上設標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)第(di)(di)(di)一(yi)(yi)、第(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)100μl，然(ran)后(hou)在(zai)(zai)第(di)(di)(di)一(yi)(yi)、第(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)50μl，混(hun)(hun)勻；然(ran)后(hou)從(cong)第(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)取(qu)100μl分(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)第(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)在(zai)(zai)第(di)(di)(di)三、第(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)50μl，混(hun)(hun)勻；然(ran)后(hou)在(zai)(zai)第(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)第(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)先(xian)各(ge)取(qu)50μl棄(qi)(qi)(qi)掉，再(zai)各(ge)取(qu)50μl分(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)五(wu)、第(di)(di)(di)六(liu)(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)在(zai)(zai)第(di)(di)(di)五(wu)、第(di)(di)(di)六(liu)(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)50ul，混(hun)(hun)勻；混(hun)(hun)勻后(hou)從(cong)第(di)(di)(di)五(wu)、第(di)(di)(di)六(liu)(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)取(qu)50μl分(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)七(qi)(qi)、第(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)在(zai)(zai)第(di)(di)(di)七(qi)(qi)、第(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)50μl，混(hun)(hun)勻后(hou)從(cong)第(di)(di)(di)七(qi)(qi)、第(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)別(bie)取(qu)50μl加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)(dao)第(di)(di)(di)九、第(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)在(zai)(zai)第(di)(di)(di)九第(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)50μl，混(hun)(hun)勻后(hou)從(cong)第(di)(di)(di)九第(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各(ge)取(qu)50μl棄(qi)(qi)(qi)掉。（稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)都為(wei)(wei)50μl，濃(nong)(nong)度(du)(du)分(fen)(fen)(fen)(fen)(fen)別(bie)為(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)別(bie)設空(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)白對照孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)及(ji)(ji)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)劑(ji)(ji)，其(qi)余各(ge)步操(cao)(cao)(cao)作(zuo)(zuo)相(xiang)同(tong)(tong)(tong)(tong)(tong)）、待(dai)測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)(ban)(ban)(ban)上待(dai)測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)40μl，然(ran)后(hou)再(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)待(dai)測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)＊終(zhong)(zhong)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)度(du)(du)為(wei)(wei)5倍(bei)(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部，盡(jin)量(liang)不(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)輕(qing)晃(huang)動(dong)混(hun)(hun)勻。3. 溫(wen)育(yu)：用(yong)(yong)封(feng)(feng)板(ban)(ban)(ban)(ban)膜封(feng)(feng)板(ban)(ban)(ban)(ban)后(hou)置(zhi)37℃溫(wen)育(yu)30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。4. 配液(ye)：將(jiang)30（48T的(de)(de)(de)(de)(de)(de)(de)(de)20倍(bei)(bei)(bei)）倍(bei)(bei)(bei)濃(nong)(nong)縮(suo)洗(xi)滌液(ye)用(yong)(yong)蒸(zheng)餾水(shui)30（48T的(de)(de)(de)(de)(de)(de)(de)(de)20倍(bei)(bei)(bei)）倍(bei)(bei)(bei)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)備用(yong)(yong)。5. 洗(xi)滌：小(xiao)心揭掉封(feng)(feng)板(ban)(ban)(ban)(ban)膜，棄(qi)(qi)(qi)去(qu)液(ye)體，甩干，每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)滿洗(xi)滌液(ye)，靜置(zhi)30秒后(hou)棄(qi)(qi)(qi)去(qu)，如(ru)此(ci)重復(fu)(fu)5次(ci)，拍干。6. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)劑(ji)(ji)50μl，空(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)除(chu)外。7. 溫(wen)育(yu)：操(cao)(cao)(cao)作(zuo)(zuo)同(tong)(tong)(tong)(tong)(tong)3。8. 洗(xi)滌：操(cao)(cao)(cao)作(zuo)(zuo)同(tong)(tong)(tong)(tong)(tong)5。9. 顯(xian)(xian)色(se)：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)顯(xian)(xian)色(se)劑(ji)(ji)A50μl，再(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)顯(xian)(xian)色(se)劑(ji)(ji)B50μl，輕(qing)輕(qing)震蕩(dang)混(hun)(hun)勻，37℃避(bi)(bi)光顯(xian)(xian)色(se)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong). 10. 終(zhong)(zhong)止(zhi)：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)止(zhi)液(ye)50μl，終(zhong)(zhong)止(zhi)反(fan)應(ying)(ying)（此(ci)時藍色(se)立轉黃色(se)）。11. 測(ce)(ce)定(ding)(ding)：以(yi)空(kong)白空(kong)調(diao)零，450nm波長依序測(ce)(ce)量(liang)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)吸光度(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)(zhi)）。 測(ce)(ce)定(ding)(ding)應(ying)(ying)在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)止(zhi)液(ye)后(hou)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)內(nei)(nei)進(jin)行(xing)(xing)(xing)。牛(niu)垂體腺(xian)苷(gan)酸(suan)環(huan)化(hua)(hua)酶(mei)(mei)激(ji)活肽(tai)27(PACAP-27)ELISA 試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)【注意事項】1． 試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)從(cong)冷(leng)藏環(huan)境中(zhong)(zhong)(zhong)(zhong)取(qu)出應(ying)(ying)在(zai)(zai)室溫(wen)平(ping)衡15-30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)方可(ke)使用(yong)(yong)，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)(ban)(ban)(ban)開封(feng)(feng)后(hou)如(ru)未用(yong)(yong)完(wan)，板(ban)(ban)(ban)(ban)條(tiao)應(ying)(ying)裝入(ru)(ru)密封(feng)(feng)袋中(zhong)(zhong)(zhong)(zhong)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。2． 濃(nong)(nong)洗(xi)滌液(ye)可(ke)能(neng)會(hui)有(you)結晶析出，稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)時可(ke)在(zai)(zai)水(shui)浴中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)溫(wen)助溶，洗(xi)滌時不(bu)影響結果。3． 各(ge)步加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)均(jun)應(ying)(ying)使用(yong)(yong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)器，并經(jing)常校對其(qi)準(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)，以(yi)避(bi)(bi)免試(shi)(shi)(shi)驗(yan)(yan)誤差。一(yi)(yi)次(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)時間＊好控(kong)制在(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)本(ben)數(shu)(shu)量(liang)多，推(tui)薦使用(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4． 請(qing)(qing)每次(ci)測(ce)(ce)定(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)同(tong)(tong)(tong)(tong)(tong)時做標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)，＊好做復(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)本(ben)中(zhong)(zhong)(zhong)(zhong)待(dai)測(ce)(ce)物(wu)(wu)質含(han)量(liang)過高(gao)（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)OD值(zhi)(zhi)(zhi)(zhi)(zhi)大于(yu)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)），請(qing)(qing)先(xian)用(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)一(yi)(yi)定(ding)(ding)倍(bei)(bei)(bei)數(shu)(shu)（n倍(bei)(bei)(bei)）后(hou)再(zai)測(ce)(ce)定(ding)(ding)，計(ji)算(suan)時請(qing)(qing)＊后(hou)乘(cheng)以(yi)總稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)（×n×5）。5． 封(feng)(feng)板(ban)(ban)(ban)(ban)膜只限(xian)一(yi)(yi)次(ci)性(xing)使用(yong)(yong)，以(yi)避(bi)(bi)免交叉污染。6． 底物(wu)(wu)請(qing)(qing)避(bi)(bi)光保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。7． 嚴格按(an)照說明書的(de)(de)(de)(de)(de)(de)(de)(de)操(cao)(cao)(cao)作(zuo)(zuo)進(jin)行(xing)(xing)(xing)，試(shi)(shi)(shi)驗(yan)(yan)結果判定(ding)(ding)必須(xu)以(yi)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)儀讀數(shu)(shu)為(wei)(wei)準(zhun)(zhun)(zhun)(zhun)(zhun).8． 所有(you)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)，洗(xi)滌液(ye)和(he)(he)各(ge)種廢(fei)棄(qi)(qi)(qi)物(wu)(wu)都應(ying)(ying)按(an)傳(chuan)染物(wu)(wu)處理。9． 本(ben)試(shi)(shi)(shi)劑(ji)(ji)不(bu)同(tong)(tong)(tong)(tong)(tong)批(pi)(pi)(pi)號組分(fen)(fen)(fen)(fen)(fen)不(bu)得混(hun)(hun)用(yong)(yong)。10. 如(ru)與(yu)英(ying)文說明書有(you)異(yi)，以(yi)英(ying)文說明書為(wei)(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)。【計(ji)算(suan)】 以(yi)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)為(wei)(wei)橫(heng)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)紙上繪出標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)，根據樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)OD 值(zhi)(zhi)(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)查出相(xiang)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)；再(zai)乘(cheng)以(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)(bei)數(shu)(shu)；或(huo)用(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)與(yu)OD值(zhi)(zhi)(zhi)(zhi)(zhi)計(ji)算(suan)出標(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)回歸方程式，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi) 代入(ru)(ru)方程式，計(ji)算(suan)出樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)濃(nong)(nong)度(du)(du)，再(zai)乘(cheng)以(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)(bei)數(shu)(shu)，即為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)實際濃(nong)(nong)度(du)(du)。 【試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)組成】試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)組成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)說明書1份1份密封(feng)(feng)袋1個1個封(feng)(feng)板(ban)(ban)(ban)(ban)膜12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)2片（96）2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)1.5ml×1瓶1.5ml×1瓶2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)劑(ji)(ji)3 ml×1瓶6 ml×1瓶2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)3 ml×1瓶6 ml×1瓶2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)顯(xian)(xian)色(se)劑(ji)(ji)A液(ye)3 ml×1瓶6 ml×1瓶2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)顯(xian)(xian)色(se)劑(ji)(ji)B液(ye)3 ml×1瓶6 ml×1瓶2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)終(zhong)(zhong)止(zhi)液(ye)3ml×1瓶6ml×1瓶2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)注意：使用(yong)(yong)前(qian)請(qing)(qing)檢(jian)查試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)劑(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)簽和(he)(he)數(shu)(shu)量(liang)與(yu)表格是否(fou)一(yi)(yi)致。【精(jing)(jing)密度(du)(du)】精(jing)(jing)密度(du)(du)用(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)測(ce)(ce)定(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)變異(yi)系數(shu)(shu)CV 表示。CV（%） = SD/mean×100批(pi)(pi)(pi)內(nei)(nei)差：取(qu)同(tong)(tong)(tong)(tong)(tong)批(pi)(pi)(pi)次(ci)試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)對低、中(zhong)(zhong)(zhong)(zhong)、高(gao)值(zhi)(zhi)(zhi)(zhi)(zhi)定(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)進(jin)行(xing)(xing)(xing)定(ding)(ding)量(liang)檢(jian)測(ce)(ce)，每份樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)連續測(ce)(ce)定(ding)(ding)20 次(ci)，分(fen)(fen)(fen)(fen)(fen)別(bie)計(ji)算(suan)不(bu)同(tong)(tong)(tong)(tong)(tong)濃(nong)(nong)度(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)的(de)(de)(de)(de)(de)(de)(de)(de)平(ping)均(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)(zhi)(zhi)。批(pi)(pi)(pi)內(nei)(nei)差： CV<9%批(pi)(pi)(pi)間差：選取(qu)3 個不(bu)同(tong)(tong)(tong)(tong)(tong)批(pi)(pi)(pi)次(ci)的(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)別(bie)對低、中(zhong)(zhong)(zhong)(zhong)、高(gao)值(zhi)(zhi)(zhi)(zhi)(zhi)定(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)進(jin)行(xing)(xing)(xing)定(ding)(ding)量(liang)測(ce)(ce)定(ding)(ding)，每個樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)使用(yong)(yong)同(tong)(tong)(tong)(tong)(tong)一(yi)(yi)試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)重復(fu)(fu)測(ce)(ce)定(ding)(ding)10 次(ci)，分(fen)(fen)(fen)(fen)(fen)別(bie)計(ji)算(suan)不(bu)同(tong)(tong)(tong)(tong)(tong)濃(nong)(nong)度(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)的(de)(de)(de)(de)(de)(de)(de)(de)平(ping)均(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)(zhi)(zhi)。批(pi)(pi)(pi)間差： CV<11% 【特(te)異(yi)性(xing)】本(ben)試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)識別(bie)天(tian)(tian)然(ran)和(he)(he)重組微生物(wu)(wu)硝酸(suan)鹽還原酶(mei)(mei)（Nar），經(jing)檢(jian)測(ce)(ce)與(yu)其(qi)它相(xiang)似物(wu)(wu)質無明顯(xian)(xian)交叉反(fan)應(ying)(ying)。由(you)于(yu)受到(dao)(dao)技(ji)術及(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)來源的(de)(de)(de)(de)(de)(de)(de)(de)限(xian)制，不(bu)可(ke)能(neng)完(wan)成所有(you)相(xiang)關(guan)或(huo)相(xiang)似物(wu)(wu)質交叉反(fan)應(ying)(ying)檢(jian)測(ce)(ce)，因(yin)此(ci)本(ben)試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)有(you)可(ke)能(neng)與(yu)未經(jing)檢(jian)測(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)其(qi)它物(wu)(wu)質有(you)交叉反(fan)應(ying)(ying)。 【穩定(ding)(ding)性(xing)】試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)在(zai)(zai)有(you)效(xiao)期內(nei)(nei)務(wu)必按(an)推(tui)薦溫(wen)度(du)(du)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)，活性(xing)降低率小(xiao)于(yu)5%。為(wei)(wei)減小(xiao)外部因(yin)素(su)對試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)破(po)壞前(qian)后(hou)檢(jian)測(ce)(ce)值(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)影響，實驗(yan)(yan)室的(de)(de)(de)(de)(de)(de)(de)(de)環(huan)境條(tiao)件需盡(jin)量(liang)保(bao)(bao)(bao)(bao)持一(yi)(yi)致，尤其(qi)是實驗(yan)(yan)室內(nei)(nei)溫(wen)度(du)(du)、濕度(du)(du)及(ji)(ji)溫(wen)育(yu)條(tiao)件。其(qi)次(ci)由(you)同(tong)(tong)(tong)(tong)(tong)一(yi)(yi)實驗(yan)(yan)員(yuan)來進(jin)行(xing)(xing)(xing)操(cao)(cao)(cao)作(zuo)(zuo)可(ke)減少(shao)人為(wei)(wei)誤差。
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