CTCF Monoclonal Antibody Catalog No DM-00964 Isotype IgG Reactivity Human;Monkey Applications WB;ELISA Gene Name CTCF Protein Name Transcriptional repressor CTCFImmunogen Purified recombinant fragment of human CTCF expressed in E. Coli. Specificity CTCF Monoclonal Antibody detects endogenous levels of CTCF protein. Formulation Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol. Source Monoclonal, Mouse Purification Affinity purificationDilution Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. Concentration 1 mg/ml Purity ≥90% Storage Stability -20°C/1 year Synonyms CTCF; Transcriptional repressor CTCF; 11-zinc finger protein; CCCTC-binding factor; CTCFL paralog Observed Band Cell Pathway Nucleus, nucleoplasm . Chromosome . Chromosome, centromere . May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase. Tissue Specificity Ubiquitous. Absent in primary spermatocytes. Function function:Transcriptional repressor binding to promoters of vertebrate c-myc gene. Also binds to the PLK and PIM1 promoters. May prevent the access of transcriptional activators to enhancers. Also acts as a transcriptional activator of APP. Involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Seems to act as tumor suppressor.,similarity:Belongs to the CTCF zinc-finger protein family.,similarity:Contains 11 C2H2-type zinc fingers.,subunit:Interacts with CHD8.,tissue specificity:Ubiquitous. Absent in primary spermatocytes.,Background This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combination
ZN143 rabbit pAb Catalog No DM-09315 Isotype IgGReactivity Human;Mouse;Rat Applications WB Gene Name ZNF143 SBF STAF Protein Name Zinc finger protein 143 (SPH-binding factor) (Selenocysteine tRNA gene transcription-activating factor) (hStaf) Immunogen Synthesized peptide derived from human ZN143 AA range: 518-568 Specificity This antibody detects endogenous levels of human ZN143 Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgG Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. Dilution WB 1:1000-2000 Concentration 1 mg/ml Purity ≥90% Storage Stability -20°C/1 year Synonyms Observed Band Cell Pathway Nucleus . Tissue Specificity Expressed in all tissues tested, with the strongest expression in ovary. Function Background matters needing attention Avoid repeated freezing and thawing! Usage suggestions This product can be used in immunological reaction related experiments. For more information, please consult technical personnel.
Elf-1 Polyclonal Antibody Catalog No DM-01689 Isotype IgGReactivity Human Applications WB;IHC;IF;ELISA Gene Name ELF1 Protein Name ETS-related transcription factor Elf-1 Immunogen The antiserum was produced against synthesized peptide derived from human ELF1. AA range:537-586 Specificity Elf-1 Polyclonal Antibody detects endogenous levels of Elf-1 protein. Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgG Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Dilution Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. Concentration 1 mg/ml Purity ≥90% Storage Stability -20°C/1 year Synonyms ELF1; ETS-related transcription factor Elf-1; E74-like factor 1 Observed Band 67kD Cell Pathway Nucleus. Tissue Specificity In fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis. Function function:Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,subunit:Binds to the underphosphorylated form of RB. May interact with other transcription factors in order to regulate specific genes. Interacts with RUNX1.,tissue specificity:In fetal tissue, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it
Kinase experiment [1]: Preparation Method Prior to use, the [8-14C] adenosine was checked for purity using isocratic HPLC elution. The assay incubation mixture contained 0.4 IU of enzyme in 50 L. The metabolites were separated by thin-layer chromatography. The radioactivity was quantitated by cutting the plastic backed TLC plates and placing them in scintillation vials, and counting in a Packard 2000 CA scintillation counter Applications IC50: 0.15 (HIV-1, A012 isolate), 0.20 μM (HIV-1, A018 isolate). 3-Deazaadenosine (hydrochloride) is an inhibitor of S-adenosylhomocysteine hydrolase, with a Ki of 3.9 μM. Cell experiment [2]: Cell lines Mouse macrophage RAW 264.7 Preparation Method RAW 264.7 cells were pretreated with or without 3-Deazaadenosine (100 μMM) for 1 h, and stimulated by the addition of LPS (1 μg/ml). After incubation for 1 h, the cells were washed, and p65 was recovered by immunoprecipitation with anti-p65 and protein A-Sepharose. Reaction Conditions 0-100 μM 3-Deazaadenosine for 1 hour Applications 3-Deazaadenosine (1-100 ?M) inhibits LPS-induced expression of TNF-α mRNA, increases DNA binding activity of NF-κB, and causes proteolytic degradation of IκBα, but Not IκBβ in RAW 264.7 cells. 3-Deazaadenosine (100 μM) enhances nuclear translocation of NF-κB, but blocks LPS-induced NF-κB transcriptional activity, and such inhibition is augmented by the addition of homocysteine. Animal experiment [3]: Animal models Male, healthy Sprague–Dawley rats (300?50 g) Preparation Method Animals and balloon injury: rats were fed for 5 days prior and 14 days after the balloon injury with standard chow containing c3Ado at a concentration of 10 mg/kg 3-Deazaadenosine body weight. Dosage form 10 mg/kg 3-Deazaadenosine for 5 days prior and 14 days after the balloon injury Applications 3-deazaadenosine (c3Ado) inhibits atherogenesis in mice. Sprague Dawley rats underwent balloon angioplasty. C3Ado was administered orally, starting 5 days prior to the balloon injury and continued for 2 weeks. Fourteen days afte
本(ben)(ben)(ben)試(shi)(shi)(shi)劑(ji)盒只(zhi)能用于(yu)科學(xue)研(yan)究(jiu)，不(bu)(bu)(bu)得(de)用于(yu)醫學(xue)診斷(duan) 細菌（Bacteria）葉酸（FA）ELISA 檢(jian)測(ce)試(shi)(shi)(shi)劑(ji)盒 使用說(shuo)明(ming)(ming)(ming)(ming)書(shu)(shu)(shu) 檢(jian)測(ce)原理 試(shi)(shi)(shi)劑(ji)盒采(cai)用雙(shuang)抗體(ti)(ti)一(yi)步夾(jia)心(xin)法酶(mei)(mei)聯(lian)免疫吸(xi)(xi)附試(shi)(shi)(shi)驗(yan)（ELISA）。往 預先包(bao)被(bei)葉酸（FA）抗體(ti)(ti)的(de)(de)(de)(de)包(bao)被(bei)微(wei)孔(kong)(kong)中(zhong)，依次加(jia)(jia)(jia)(jia)入(ru)標(biao)本(ben)(ben)(ben)、 標(biao)準(zhun)(zhun)(zhun)(zhun)品、HRP標(biao)記的(de)(de)(de)(de)檢(jian)測(ce)抗體(ti)(ti)，經過(guo)(guo)溫育并徹(che)底(di)洗(xi)(xi)(xi)滌(di)(di)。用底(di)物(wu)(wu)TMB顯 色(se)，TMB在(zai)(zai)(zai)(zai)過(guo)(guo)氧化(hua)(hua)物(wu)(wu)酶(mei)(mei)的(de)(de)(de)(de)催(cui)化(hua)(hua)下轉化(hua)(hua)成藍色(se)，并在(zai)(zai)(zai)(zai)酸的(de)(de)(de)(de)作(zuo)(zuo)用下轉化(hua)(hua)成 ＊終的(de)(de)(de)(de)黃色(se)。顏色(se)的(de)(de)(de)(de)深淺和樣(yang)(yang)品中(zhong)的(de)(de)(de)(de)葉酸（FA）呈正相關(guan)。 用酶(mei)(mei)標(biao)儀(yi)在(zai)(zai)(zai)(zai)450nm 波長下測(ce)定(ding)(ding)吸(xi)(xi)光度（OD 值(zhi)），計(ji)算(suan)樣(yang)(yang)品濃(nong)(nong)度。樣(yang)(yang)品收集(ji)(ji)、處(chu)理及(ji)(ji)保(bao)存方法1. 血(xue)(xue)清(qing)(qing)：使用不(bu)(bu)(bu)含(han)熱(re)原和內毒素的(de)(de)(de)(de)試(shi)(shi)(shi)管，操(cao)(cao)作(zuo)(zuo)過(guo)(guo)程中(zhong)避(bi)免任何(he)細 胞(bao)刺激，收集(ji)(ji)血(xue)(xue)液(ye)(ye)后(hou)(hou)，3000 轉離(li)心(xin) 10 分(fen)(fen)鐘將血(xue)(xue)清(qing)(qing)和紅細胞(bao)迅(xun)速小(xiao)(xiao)心(xin) 地分(fen)(fen)離(li)。 2. 血(xue)(xue)漿：EDTA、檸檬酸鹽或(huo)肝素抗凝。3000 轉離(li)心(xin) 30 分(fen)(fen)鐘取上(shang)(shang)清(qing)(qing)。3. 細胞(bao)上(shang)(shang)清(qing)(qing)液(ye)(ye)：3000 轉離(li)心(xin) 10 分(fen)(fen)鐘去(qu)除(chu)顆(ke)粒和聚合物(wu)(wu)。 4. 組織(zhi)勻漿：將組織(zhi)加(jia)(jia)(jia)(jia)入(ru)適(shi)量生理鹽水(shui)(shui)搗碎。3000 轉離(li)心(xin) 10 分(fen)(fen)鐘 取上(shang)(shang)清(qing)(qing)。 5. 保(bao)存：如果樣(yang)(yang)本(ben)(ben)(ben)收集(ji)(ji)后(hou)(hou)不(bu)(bu)(bu)及(ji)(ji)時(shi)(shi)檢(jian)測(ce)，請按一(yi)次用量分(fen)(fen)裝(zhuang)，凍(dong)存 于(yu)-20℃，避(bi)免反復(fu)凍(dong)融(rong)，在(zai)(zai)(zai)(zai)室溫下解凍(dong)并確(que)保(bao)樣(yang)(yang)品均勻地充分(fen)(fen)解凍(dong)。 自備物(wu)(wu)品 1. 酶(mei)(mei)標(biao)儀(yi)（450nm）2. 高精度加(jia)(jia)(jia)(jia)樣(yang)(yang)器及(ji)(ji)槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL 3. 37℃恒溫箱 操(cao)(cao)作(zuo)(zuo)注意事(shi)項 1. 試(shi)(shi)(shi)劑(ji)盒保(bao)存在(zai)(zai)(zai)(zai) 2-8℃，使用前室溫平衡 20 分(fen)(fen)鐘。從冰箱取出的(de)(de)(de)(de) 濃(nong)(nong)縮洗(xi)(xi)(xi)滌(di)(di)液(ye)(ye)會有(you)結(jie)晶，這屬于(yu)正常現(xian)象，水(shui)(shui)浴加(jia)(jia)(jia)(jia)熱(re)使結(jie)晶完全溶解 后(hou)(hou)再(zai)使用。2. 實驗(yan)中(zhong)不(bu)(bu)(bu)用的(de)(de)(de)(de)板(ban)(ban)(ban)(ban)(ban)條(tiao)應(ying)立即放回自封(feng)袋(dai)中(zhong)，密(mi)封(feng)（低(di)溫干(gan)燥）保(bao) 存。 3. 濃(nong)(nong)度為 0 的(de)(de)(de)(de) S0 號標(biao)準(zhun)(zhun)(zhun)(zhun)品即可視為陰性(xing)對照或(huo)者(zhe)(zhe)空(kong)白(bai)；按照說(shuo)明(ming)(ming)(ming)(ming) 書(shu)(shu)(shu)操(cao)(cao)作(zuo)(zuo)時(shi)(shi)樣(yang)(yang)本(ben)(ben)(ben)已經稀(xi)(xi)(xi)釋(shi)(shi) 5 倍，＊終結(jie)果乘以 5 才是樣(yang)(yang)本(ben)(ben)(ben)實際濃(nong)(nong)度。4. 嚴格按照說(shuo)明(ming)(ming)(ming)(ming)書(shu)(shu)(shu)中(zhong)標(biao)明(ming)(ming)(ming)(ming)的(de)(de)(de)(de)時(shi)(shi)間、加(jia)(jia)(jia)(jia)液(ye)(ye)量及(ji)(ji)順(shun)序進行溫育操(cao)(cao)作(zuo)(zuo)。 5. 所有(you)液(ye)(ye)體(ti)(ti)組分(fen)(fen)使用前充分(fen)(fen)搖勻。試(shi)(shi)(shi)劑(ji)盒組成96孔(kong)(kong)配置(zhi)48孔(kong)(kong)配置(zhi)備注微(wei)孔(kong)(kong)酶(mei)(mei)標(biao)版12孔(kong)(kong)*8條(tiao)12孔(kong)(kong)*4條(tiao)無(wu)(wu)標(biao)準(zhun)(zhun)(zhun)(zhun)品0.3ml*6管0.3ml*6管無(wu)(wu)樣(yang)(yang)本(ben)(ben)(ben)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)6ml3ml無(wu)(wu)檢(jian)測(ce)抗體(ti)(ti)-HRP10ml5ml無(wu)(wu)20*洗(xi)(xi)(xi)滌(di)(di)緩(huan)沖(chong)液(ye)(ye)25ml15ml按說(shuo)明(ming)(ming)(ming)(ming)書(shu)(shu)(shu)進行稀(xi)(xi)(xi)釋(shi)(shi)底(di)物(wu)(wu)A6ml3ml無(wu)(wu)底(di)物(wu)(wu)B6ml3ml無(wu)(wu)終止液(ye)(ye)6ml3ml無(wu)(wu)封(feng)板(ban)(ban)(ban)(ban)(ban)膜2張(zhang)(zhang)2張(zhang)(zhang)無(wu)(wu)說(shuo)明(ming)(ming)(ming)(ming)書(shu)(shu)(shu)1份(fen)(fen)1份(fen)(fen)無(wu)(wu)自封(feng)袋(dai)1個(ge)1個(ge)無(wu)(wu)注：標(biao)準(zhun)(zhun)(zhun)(zhun)品（S0-S5）濃(nong)(nong)度依次為：0、10、20、40、80、160 ng/ml 試(shi)(shi)(shi)劑(ji)的(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)備 20×洗(xi)(xi)(xi)滌(di)(di)緩(huan)沖(chong)液(ye)(ye)的(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)：蒸餾水(shui)(shui)按 1：20 稀(xi)(xi)(xi)釋(shi)(shi)，即 1 份(fen)(fen)的(de)(de)(de)(de) 20×洗(xi)(xi)(xi)滌(di)(di) 緩(huan)沖(chong)液(ye)(ye)加(jia)(jia)(jia)(jia) 19 份(fen)(fen)的(de)(de)(de)(de)蒸餾水(shui)(shui)。 洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)方法 1. 手工(gong)洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)：甩盡孔(kong)(kong)內液(ye)(ye)體(ti)(ti)，每(mei)孔(kong)(kong)加(jia)(jia)(jia)(jia)滿(man)洗(xi)(xi)(xi)滌(di)(di)液(ye)(ye)，靜置(zhi) 1min 后(hou)(hou)甩盡 孔(kong)(kong)內液(ye)(ye)體(ti)(ti)，在(zai)(zai)(zai)(zai)吸(xi)(xi)水(shui)(shui)紙(zhi)上(shang)(shang)拍(pai)干(gan)，如此洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban) 5 次。 2. 自動洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)機：每(mei)孔(kong)(kong)注入(ru)洗(xi)(xi)(xi)液(ye)(ye) 350μL，浸泡 1min，洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban) 5 次。 操(cao)(cao)作(zuo)(zuo)步驟 1. 從室溫平衡 20min 后(hou)(hou)的(de)(de)(de)(de)鋁箔(bo)袋(dai)中(zhong)取出所需板(ban)(ban)(ban)(ban)(ban)條(tiao)，剩余板(ban)(ban)(ban)(ban)(ban)條(tiao)用自 封(feng)袋(dai)密(mi)封(feng)放回 4℃。 2. 設置(zhi)標(biao)準(zhun)(zhun)(zhun)(zhun)品孔(kong)(kong)和樣(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)，標(biao)準(zhun)(zhun)(zhun)(zhun)品孔(kong)(kong)各加(jia)(jia)(jia)(jia)不(bu)(bu)(bu)同(tong)濃(nong)(nong)度的(de)(de)(de)(de)標(biao)準(zhun)(zhun)(zhun)(zhun)品 50μ L；3. 樣(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)先加(jia)(jia)(jia)(jia)待(dai)測(ce)樣(yang)(yang)本(ben)(ben)(ben) 10μL，再(zai)加(jia)(jia)(jia)(jia)樣(yang)(yang)本(ben)(ben)(ben)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye) 40μL(即樣(yang)(yang)本(ben)(ben)(ben)稀(xi)(xi)(xi) 釋(shi)(shi) 5 倍)；空(kong)白(bai)孔(kong)(kong)不(bu)(bu)(bu)加(jia)(jia)(jia)(jia)。4. 除(chu)空(kong)白(bai)孔(kong)(kong)外，標(biao)準(zhun)(zhun)(zhun)(zhun)品孔(kong)(kong)和樣(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)中(zhong)每(mei)孔(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)辣根過(guo)(guo)氧化(hua)(hua)物(wu)(wu)酶(mei)(mei) （HRP）標(biao)記的(de)(de)(de)(de)檢(jian)測(ce)抗體(ti)(ti) 100μL，用封(feng)板(ban)(ban)(ban)(ban)(ban)膜封(feng)住(zhu)反應(ying)孔(kong)(kong)，37℃水(shui)(shui)浴鍋(guo) 或(huo)恒溫箱溫育 60min。 5. 棄去(qu)液(ye)(ye)體(ti)(ti)，吸(xi)(xi)水(shui)(shui)紙(zhi)上(shang)(shang)拍(pai)干(gan)，每(mei)孔(kong)(kong)加(jia)(jia)(jia)(jia)滿(man)洗(xi)(xi)(xi)滌(di)(di)液(ye)(ye)，靜置(zhi) 1min，甩去(qu) 洗(xi)(xi)(xi)滌(di)(di)液(ye)(ye)，吸(xi)(xi)水(shui)(shui)紙(zhi)上(shang)(shang)拍(pai)干(gan)，如此重復(fu)洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban) 5 次（也可用洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)機洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)）。6. 每(mei)孔(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)底(di)物(wu)(wu) A、B 各 50μL，37℃避(bi)光孵(fu)育 15min。7. 每(mei)孔(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)終止液(ye)(ye) 50μL，15min 內，在(zai)(zai)(zai)(zai) 450nm 波長處(chu)測(ce)定(ding)(ding)各孔(kong)(kong)的(de)(de)(de)(de) OD 值(zhi)。結(jie)果判斷(duan)繪制標(biao)準(zhun)(zhun)(zhun)(zhun)曲線(xian)：在(zai)(zai)(zai)(zai) Excel 工(gong)作(zuo)(zuo)表中(zhong)，以標(biao)準(zhun)(zhun)(zhun)(zhun)品濃(nong)(nong)度作(zuo)(zuo)橫坐(zuo)標(biao)，對應(ying) OD 值(zhi)作(zuo)(zuo)縱坐(zuo)標(biao)，繪制出標(biao)準(zhun)(zhun)(zhun)(zhun)品線(xian)性(xing)回歸曲線(xian)，按曲線(xian)方程計(ji)算(suan)各樣(yang)(yang) 本(ben)(ben)(ben)濃(nong)(nong)度值(zhi)。試(shi)(shi)(shi)劑(ji)盒性(xing)能 1. 準(zhun)(zhun)(zhun)(zhun)確(que)性(xing)：標(biao)準(zhun)(zhun)(zhun)(zhun)品線(xian)性(xing)回歸與預期(qi)濃(nong)(nong)度相關(guan)系數 R 值(zhi)，大(da)于(yu)等于(yu) 0.9900。2. 靈敏度：＊低(di)檢(jian)測(ce)濃(nong)(nong)度小(xiao)(xiao)于(yu) 1.0 ng/ml。 3. 特異(yi)性(xing)：不(bu)(bu)(bu)與其(qi)它可溶性(xing)結(jie)構類似(si)物(wu)(wu)交(jiao)叉反應(ying)。4. 重復(fu)性(xing)：板(ban)(ban)(ban)(ban)(ban)內、板(ban)(ban)(ban)(ban)(ban)間變異(yi)系數均小(xiao)(xiao)于(yu) 15%。 5. 貯藏(zang)：2-8℃，避(bi)光防潮保(bao)存。6. 有(you)效期(qi)：6 個(ge)月 免責聲明(ming)(ming)(ming)(ming) 1. 試(shi)(shi)(shi)劑(ji)盒僅供(gong)研(yan)究(jiu)使用，不(bu)(bu)(bu)得(de)用于(yu)臨床實驗(yan)或(huo)人(ren)體(ti)(ti)實驗(yan)，否則所 產生的(de)(de)(de)(de)一(yi)切后(hou)(hou)果，由實驗(yan)者(zhe)(zhe)承擔，本(ben)(ben)(ben)公司概(gai)不(bu)(bu)(bu)負責。 2. 嚴格按照說(shuo)明(ming)(ming)(ming)(ming)書(shu)(shu)(shu)操(cao)(cao)作(zuo)(zuo)，實驗(yan)者(zhe)(zhe)違反說(shuo)明(ming)(ming)(ming)(ming)書(shu)(shu)(shu)操(cao)(cao)作(zuo)(zuo)，后(hou)(hou)果由實驗(yan)者(zhe)(zhe)承擔細菌（Bacteria）葉酸（FA）ELISA 檢(jian)測(ce)試(shi)(shi)(shi)劑(ji)盒FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. urobilin ELISA Kit instruction Intended use This urobilin ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of urobilin in the sample, this urobilin ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus urobilin concentration. The concentration of urobilin in the samples is then determined by comparing the O.D. of the samples to the standard curve. Sample collection and storages Serum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cycles Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles. Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed. Materials required but not supplied 1. Standard microplate reader(450nm) 2. Precision pipettes and Disposable pipette tips. 3. 37 ℃ incubator Precautions1. Do not substitute reagents from one kit to another. Standard, conjugate microplates are matched for optimal performance. Use only the reagents supplied by manufacturer. 2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided. 3. Mix all reagents before using. Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C) Materials supplied Name 96 determinations 48 determinations Microelisa stripplate 12*8strips 12*4strips Standard 0.3ml*6tubes 0.3ml*6tubes Sample Diluent 6.0ml 3.0ml HRP-Conjugate reagent 10.0ml 5.0ml 20X Wash solution 25ml 15ml Chromogen Solution A 6.0ml 3.0ml Chromogen Solution B 6.0ml 3.0ml Stop Solution 6.0ml 3.0ml Closure plate membrane 2 2 User manual 1 1 Sealed bags 1 1Note: Standard (S0 → S5) concentration was followed by:0,10,20,40,80,160 ng/ml Reagent preparation 20×wash solution:Dilute with Distilled or deionized water 1:20.Assay procedure1. Prepare all r e a g e n ts before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well. 3. Add Sample: Add testing sample 10μl then add 40μl of Sample Diluent to testing sample well; Blank well doesn’t add anyting. 4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Washfllygs.com Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels. 6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light. 7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing. 8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes. Calculation of results1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis.2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software.3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve. 5. The sensitivity by this assay is 1.0 ng/ml 6. Standard curvefllygs.com Storage： 2-8℃. validity： six months. FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
植(zhi)物組織蔗(zhe)(zhe)(zhe)(zhe)糖含(han)(han)量(liang)(liang)檢(jian)(jian)測(ce)(ce)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒說明書(shu) 微(wei)量(liang)(liang)法 注(zhu)意：正(zheng)式測(ce)(ce)定(ding)前務必取(qu) 2-3 個(ge)預(yu)期差異(yi)較大的(de)樣(yang)本(ben)(ben)做預(yu)測(ce)(ce)定(ding)。 貨(huo)號：YX-W-B502 規格：100T/96S 產品(pin)內容： 提取(qu)液(ye)(ye)：液(ye)(ye)體 100mL×1 瓶，4℃保存； 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)一(yi)：粉(fen)劑(ji)(ji)(ji)(ji) 10mg×1 支，4℃保存，臨用(yong)前加(jia)入(ru) 1mL 蒸餾(liu)(liu)水(shui)溶解(jie)，用(yong)水(shui)稀釋 10 倍，備(bei)用(yong)，即 1mg/mL; 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)二：液(ye)(ye)體 2mL×1 瓶，4℃保存； 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)三：液(ye)(ye)體 20mL×1 瓶，4℃保存； 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)四：液(ye)(ye)體 5mL×1 瓶，4℃保存； 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)五(wu)(wu)：粉(fen)劑(ji)(ji)(ji)(ji) 0.5g×1 瓶，常溫(wen)保存。 植(zhi)物組織蔗(zhe)(zhe)(zhe)(zhe)糖含(han)(han)量(liang)(liang)檢(jian)(jian)測(ce)(ce)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒說明書(shu) 產品(pin)介紹(shao)： 蔗(zhe)(zhe)(zhe)(zhe)糖是(shi)植(zhi)物光合作用(yong)的(de)主要(yao)產物，也是(shi)糖分(fen)(fen)運(yun)輸和(he)儲藏的(de)主要(yao)形式。因此，測(ce)(ce)定(ding)蔗(zhe)(zhe)(zhe)(zhe)糖含(han)(han)量(liang)(liang)對 于植(zhi)物糖代謝具有重(zhong)(zhong)要(yao)意義。此外，蔗(zhe)(zhe)(zhe)(zhe)糖含(han)(han)量(liang)(liang)是(shi)飲料﹑蜂蜜、果(guo)脯﹑糖果(guo)和(he)乳制品(pin)等產品(pin)質(zhi)量(liang)(liang)控 制的(de)重(zhong)(zhong)要(yao)指(zhi)標(biao)(biao)(biao)之(zhi)一(yi)。 先用(yong)堿(jian)與(yu)樣(yang)品(pin)共熱，破壞(huai)其(qi)中(zhong)的(de)還(huan)原糖。酸性條件(jian)下(xia)蔗(zhe)(zhe)(zhe)(zhe)糖水(shui)解(jie)生成葡萄糖和(he)果(guo)糖，果(guo)糖進一(yi) 步與(yu)間苯二酚反應，生成有色(se)物質(zhi)，在 480nm 下(xia)有特征吸(xi)(xi)收(shou)峰(feng)。 所需的(de)儀(yi)(yi)器和(he)用(yong)品(pin)： 可見分(fen)(fen)光光度(du)計/酶標(biao)(biao)(biao)儀(yi)(yi)、水(shui)浴鍋(guo)、可調(diao)式移液(ye)(ye)器、微(wei)量(liang)(liang)玻璃比色(se)皿/96 孔板和(he)蒸餾(liu)(liu)水(shui)。 操作步驟 一(yi)、樣(yang)品(pin)制備(bei)： 稱取(qu)0.1g 樣(yang)本(ben)(ben)，常溫(wen)研碎，加(jia)入(ru)0.5mL 提取(qu)液(ye)(ye)，適當研磨后快(kuai)速(su)轉移到離心管(guan)(guan)中(zhong)，置于80℃水(shui)浴鍋(guo)中(zhong) 10min，振蕩3~5 次(ci)(ci)，冷卻后，4000g，25℃離心10min，取(qu)上(shang)清(qing)，加(jia)入(ru)2mg試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)五(wu)(wu)，80℃脫色(se)30min， 再加(jia)入(ru)0.5mL 提取(qu)液(ye)(ye)，4000g，25℃離心10min，取(qu)上(shang)清(qing)液(ye)(ye)測(ce)(ce)定(ding)。 二、測(ce)(ce)定(ding)步驟： 1、分(fen)(fen)光光度(du)計或酶標(biao)(biao)(biao)儀(yi)(yi)預(yu)熱 30min 以(yi)上(shang)，調(diao)節波長(chang)至 480nm，蒸餾(liu)(liu)水(shui)調(diao)零(ling)。 2、樣(yang)本(ben)(ben)測(ce)(ce)定(ding)，（在 1.5mL EP 管(guan)(guan)中(zhong)依次(ci)(ci)加(jia)入(ru)下(xia)列試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)）： 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)（μL）空白管(guan)(guan)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)管(guan)(guan)測(ce)(ce)定(ding)管(guan)(guan)樣(yang)本(ben)(ben)25 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)一(yi)25 蒸餾(liu)(liu)水(shui)25 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)二151515混勻，100℃煮沸(fei) 5min 左右（蓋緊(jin)，防止(zhi)水(shui)分(fen)(fen)散失）試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)三175175175試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)四505050混勻，沸(fei)水(shui)浴反應 10min 左右，冷卻后取(qu) 200μL 至微(wei)量(liang)(liang)玻璃比色(se)皿或 96 孔板中(zhong)測(ce)(ce)定(ding) 480nm 處光吸(xi)(xi) 收(shou)值，空白管(guan)(guan)、標(biao)(biao)(biao)準(zhun)(zhun)(zhun)管(guan)(guan)和(he)測(ce)(ce)定(ding)管(guan)(guan)分(fen)(fen)別(bie)記為(wei) A1、A2 和(he) A3 三、蔗(zhe)(zhe)(zhe)(zhe)糖含(han)(han)量(liang)(liang)計算(suan)： 1、按照蛋白質(zhi)含(han)(han)量(liang)(liang)計算(suan) 蔗(zhe)(zhe)(zhe)(zhe)糖含(han)(han)量(liang)(liang)（mg/mg prot）= (C 標(biao)(biao)(biao)準(zhun)(zhun)(zhun)管(guan)(guan)×V1)×(A3-A1)÷(A2-A1) ÷（V1×Cpr）= (A3-A1)÷(A2-A1) ÷Cpr 此法需要(yao)自行(xing)測(ce)(ce)定(ding)蛋白濃度(du)。 2、按照樣(yang)品(pin)質(zhi)量(liang)(liang)計算(suan) 蔗(zhe)(zhe)(zhe)(zhe)糖含(han)(han)量(liang)(liang)(mg/g 鮮重(zhong)(zhong)）=(C 標(biao)(biao)(biao)準(zhun)(zhun)(zhun)×V1)×(A3-A1)÷(A2-A1)÷(W×V1÷V2)=(A3-A1)÷(A2-A1)÷W C 標(biao)(biao)(biao)準(zhun)(zhun)(zhun)管(guan)(guan)：標(biao)(biao)(biao)準(zhun)(zhun)(zhun)管(guan)(guan)濃度(du)，1mg/mL；V1：加(jia)入(ru)樣(yang)本(ben)(ben)體積(ji)，0.025mL；V2：加(jia)入(ru)提取(qu)液(ye)(ye)體積(ji)，1mL； Cpr：樣(yang)本(ben)(ben)蛋白質(zhi)濃度(du)，mg/mL；W：樣(yang)本(ben)(ben)鮮重(zhong)(zhong)，g。 本(ben)(ben)產品(pin)僅供科(ke)學研究使用(yong)！請勿(wu)用(yong)于臨床、診斷(duan)、食(shi)品(pin)、化妝品(pin)檢(jian)(jian)測(ce)(ce)等用(yong)途！
實(shi)驗原理 試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)采(cai)(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙(shuang)抗(kang)(kang)體一(yi)步(bu)(bu)夾(jia)心法酶(mei)(mei)(mei)(mei)聯(lian)免(mian)疫吸(xi)附試(shi)(shi)(shi)驗（ELISA）。往預(yu)先包被植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)多(duo)(duo)(duo)糖(Pol)捕獲(huo)抗(kang)(kang)體的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)(kong)(kong)中(zhong)(zhong)(zhong)，依次(ci)加(jia)(jia)(jia)(jia)(jia)入(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)體，經過(guo)(guo)溫(wen)育并徹底(di)(di)洗(xi)(xi)滌。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)TMB顯(xian)色(se)，TMB在(zai)(zai)(zai)過(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)(hua)下(xia)(xia)轉化(hua)(hua)(hua)成(cheng)(cheng)藍色(se)，并在(zai)(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)下(xia)(xia)轉化(hua)(hua)(hua)成(cheng)(cheng)＊終(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)。顏色(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)多(duo)(duo)(duo)糖(Pol)呈(cheng)正相(xiang)關(guan)(guan)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)(zai)450nm 波長下(xia)(xia)測(ce)(ce)(ce)(ce)(ce)(ce)定吸(xi)光度(du)(du)(du)(du)(du)(du)（OD 值(zhi)(zhi)），計(ji)(ji)算(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃度(du)(du)(du)(du)(du)(du)。保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)條件(jian)及(ji)有(you)(you)(you)效期(qi)1.本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)體外定量(liang)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)血(xue)清(qing)、血(xue)漿、組織(zhi)勻(yun)漿及(ji)相(xiang)關(guan)(guan)液(ye)(ye)(ye)(ye)(ye)體樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)多(duo)(duo)(duo)糖(Pol)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)；2.試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)：2-8℃；3.有(you)(you)(you)效期(qi)：6個(ge)月(yue)(yue)。植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)多(duo)(duo)(duo)糖(Pol)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)(cai)集(ji)及(ji)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)1.血(xue)清(qing)：全血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)請(qing)(qing)(qing)(qing)于(yu)(yu)(yu)室溫(wen)放(fang)(fang)置2小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)(huo)(huo)4℃過(guo)(guo)一(yi)晚后(hou)(hou)(hou)于(yu)(yu)(yu)1000 x g離心20分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，取(qu)上(shang)(shang)清(qing)即(ji)(ji)可(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)(fang)于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避免(mian)反(fan)(fan)復(fu)凍(dong)融(rong)(rong)。 2.血(xue)漿：可(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)(huo)肝素(su)(su)作(zuo)為(wei)(wei)(wei)(wei)抗(kang)(kang)凝劑(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)采(cai)(cai)集(ji)后(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)于(yu)(yu)(yu)2 - 8° C 1000 x g離心15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，或(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)(fang)于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避免(mian)反(fan)(fan)復(fu)凍(dong)融(rong)(rong)。 3.細胞(bao)培養物(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)清(qing)或(huo)(huo)(huo)(huo)(huo)其它生物(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)：1000 x g離心20分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，取(qu)上(shang)(shang)清(qing)即(ji)(ji)可(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)(fang)于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避免(mian)反(fan)(fan)復(fu)凍(dong)融(rong)(rong)。 (注(zhu)(zhu)(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)溶(rong)(rong)血(xue)會影響**檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)結果(guo)，因此溶(rong)(rong)血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)不(bu)(bu)宜進(jin)行(xing)此項檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)。) 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)多(duo)(duo)(duo)糖(Pol)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he) 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原則(ze)(ze)：首先通過(guo)(guo)文(wen)獻檢(jian)(jian)(jian)(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)式了(le)解(jie)待測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大(da)致(zhi)含量(liang)，確(que)定適當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)。只(zhi)有(you)(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍(wei)內(nei)，檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結果(guo)才(cai)是(shi)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)(zhong)(zhong)，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)做好詳細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)錄(lu)。計(ji)(ji)算(suan)(suan)濃度(du)(du)(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)了(le)“N”倍(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃度(du)(du)(du)(du)(du)(du)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)再(zai)(zai)乘(cheng)以“N”。 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原則(ze)(ze)：2瓶(ping)，每(mei)(mei)(mei)(mei)瓶(ping)臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至1ml，蓋(gai)好后(hou)(hou)(hou)靜置10分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以上(shang)(shang)，然后(hou)(hou)(hou)反(fan)(fan)復(fu)顛倒/搓(cuo)動以助溶(rong)(rong)解(jie)，其濃度(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)300 pg/ml，做系(xi)列倍(bei)(bei)比(bi)(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)直(zhi)接作(zuo)為(wei)(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃度(du)(du)(du)(du)(du)(du)0 pg/ml，臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 如(ru)(ru)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取(qu)0.5ml（不(bu)(bu)要(yao)少于(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)入(ru)含0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中(zhong)(zhong)(zhong)，混(hun)(hun)(hun)勻(yun)即(ji)(ji)可(ke)(ke)，其余濃度(du)(du)(du)(du)(du)(du)以此類推(tui)。 生物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原則(ze)(ze)：臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以生物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)根(gen)(gen)(gen)(gen)據(ju)(ju)(ju)預(yu)先計(ji)(ji)算(suan)(suan)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)實(shi)驗所(suo)需(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)100μl），實(shi)際配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)多(duo)(duo)(duo)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl生物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體加(jia)(jia)(jia)(jia)(jia)990μl生物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)例(li)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)(hun)勻(yun)，在(zai)(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)素(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原則(ze)(ze)：臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)素(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)根(gen)(gen)(gen)(gen)據(ju)(ju)(ju)預(yu)先計(ji)(ji)算(suan)(suan)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)實(shi)驗所(suo)需(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)100μl），實(shi)際配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)多(duo)(duo)(duo)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)素(su)(su)加(jia)(jia)(jia)(jia)(jia)990μl辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)素(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)例(li)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)(hun)勻(yun)，在(zai)(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)多(duo)(duo)(duo)糖(Pol)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)操作(zuo)步(bu)(bu)驟 實(shi)驗開(kai)始前(qian)(qian)(qian)(qian)，請(qing)(qing)(qing)(qing)提前(qian)(qian)(qian)(qian)配(pei)(pei)(pei)(pei)(pei)置好所(suo)有(you)(you)(you)試(shi)(shi)(shi)劑(ji)(ji)(ji)，試(shi)(shi)(shi)劑(ji)(ji)(ji)或(huo)(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)，均需(xu)(xu)(xu)混(hun)(hun)(hun)勻(yun)，混(hun)(hun)(hun)勻(yun)時(shi)(shi)(shi)(shi)(shi)(shi)盡(jin)(jin)量(liang)避免(mian)起泡(pao)。每(mei)(mei)(mei)(mei)次(ci)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)(ying)(ying)(ying)該做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)。如(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃度(du)(du)(du)(du)(du)(du)過(guo)(guo)高(gao)時(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)進(jin)行(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，以使(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)符(fu)合試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)范圍(wei)。 1.加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)設(she)空白(bai)孔(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)、待測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)。空白(bai)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)(huo)待測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)(zhu)(zhu)意(yi)不(bu)(bu)要(yao)有(you)(you)(you)氣泡(pao)，加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)于(yu)(yu)(yu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)(kong)(kong)底(di)(di)部，盡(jin)(jin)量(liang)不(bu)(bu)觸及(ji)孔(kong)(kong)(kong)壁，輕(qing)(qing)(qing)輕(qing)(qing)(qing)晃動混(hun)(hun)(hun)勻(yun)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)加(jia)(jia)(jia)(jia)(jia)上(shang)(shang)蓋(gai)或(huo)(huo)(huo)(huo)(huo)覆膜(mo)，37℃反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)(wei)保(bao)(bao)(bao)(bao)證(zheng)實(shi)驗結果(guo)有(you)(you)(you)效性(xing)，每(mei)(mei)(mei)(mei)次(ci)實(shi)驗請(qing)(qing)(qing)(qing)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)。 2.棄(qi)去(qu)液(ye)(ye)(ye)(ye)(ye)體，甩干(gan)，不(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)滌。每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)生物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體工(gong)(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye) 100μl（取(qu)1μl生物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體加(jia)(jia)(jia)(jia)(jia)99μl生物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)例(li)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)(hun)勻(yun)，在(zai)(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 3.溫(wen)育60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)，棄(qi)去(qu)孔(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)體，甩干(gan)，洗(xi)(xi)板(ban)(ban)3次(ci)，每(mei)(mei)(mei)(mei)次(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)，甩干(gan)。4.每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)素(su)(su)工(gong)(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)（同(tong)生物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體工(gong)(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。5.溫(wen)育60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)，棄(qi)去(qu)孔(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)體，甩干(gan)，洗(xi)(xi)板(ban)(ban)5次(ci)，每(mei)(mei)(mei)(mei)次(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)，甩干(gan)。6.依序每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)90μl，37℃避光顯(xian)色(se)（30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)，此時(shi)(shi)(shi)(shi)(shi)(shi)肉(rou)眼可(ke)(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)(qian)3-4孔(kong)(kong)(kong)有(you)(you)(you)明顯(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)(du)(du)(du)藍色(se)，后(hou)(hou)(hou)3-4孔(kong)(kong)(kong)梯度(du)(du)(du)(du)(du)(du)不(bu)(bu)明顯(xian)，即(ji)(ji)可(ke)(ke)終(zhong)(zhong)止(zhi)(zhi)）。7.依序每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)止(zhi)(zhi)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)止(zhi)(zhi)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)（此時(shi)(shi)(shi)(shi)(shi)(shi)藍色(se)立轉黃色(se)）。終(zhong)(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)入(ru)順序應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)(jin)量(liang)與(yu)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)入(ru)順序相(xiang)同(tong)。為(wei)(wei)(wei)(wei)了(le)保(bao)(bao)(bao)(bao)證(zheng)實(shi)驗結果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)，底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)間到(dao)后(hou)(hou)(hou)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)(jin)快加(jia)(jia)(jia)(jia)(jia)入(ru)終(zhong)(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)聯(lian)儀在(zai)(zai)(zai)450nm波長依序測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)各孔(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光密度(du)(du)(du)(du)(du)(du)（OD值(zhi)(zhi)）。 在(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以內(nei)進(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)。植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)多(duo)(duo)(duo)糖(Pol)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)注(zhu)(zhu)(zhu)意(yi)事項1.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)(zai)初次(ci)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)時(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)將(jiang)(jiang)(jiang)(jiang)各種試(shi)(shi)(shi)劑(ji)(ji)(ji)管離心數(shu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，以便試(shi)(shi)(shi)劑(ji)(ji)(ji)集(ji)中(zhong)(zhong)(zhong)到(dao)管底(di)(di)。2.每(mei)(mei)(mei)(mei)次(ci)實(shi)驗留一(yi)孔(kong)(kong)(kong)作(zuo)為(wei)(wei)(wei)(wei)空白(bai)調(diao)零孔(kong)(kong)(kong)，該孔(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)(jia)任何(he)試(shi)(shi)(shi)劑(ji)(ji)(ji)，只(zhi)是(shi)**加(jia)(jia)(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)及(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)時(shi)(shi)(shi)(shi)(shi)(shi)先用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)此孔(kong)(kong)(kong)調(diao)OD值(zhi)(zhi)至零。 3.為(wei)(wei)(wei)(wei)防止(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸(zheng)發，試(shi)(shi)(shi)驗時(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)(jiang)(jiang)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)板(ban)(ban)放(fang)(fang)于(yu)(yu)(yu)鋪有(you)(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉(bi)盒(he)(he)(he)(he)(he)(he)(he)內(nei)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)加(jia)(jia)(jia)(jia)(jia)上(shang)(shang)蓋(gai)或(huo)(huo)(huo)(huo)(huo)覆膜(mo)。 4.未使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)或(huo)(huo)(huo)(huo)(huo)者(zhe)試(shi)(shi)(shi)劑(ji)(ji)(ji)，請(qing)(qing)(qing)(qing)于(yu)(yu)(yu)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體工(gong)(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)、辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)素(su)(su)工(gong)(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)請(qing)(qing)(qing)(qing)依據(ju)(ju)(ju)所(suo)需(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)配(pei)(pei)(pei)(pei)(pei)置使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)(qing)(qing)勿重(zhong)復(fu)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體工(gong)(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)(huo)、辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)素(su)(su)工(gong)(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)。 5.建(jian)議檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)均設(she)雙(shuang)孔(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)定，以保(bao)(bao)(bao)(bao)證(zheng)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)結果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)。 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)多(duo)(duo)(duo)糖(Pol)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)洗(xi)(xi)板(ban)(ban)方(fang)法手工(gong)(gong)(gong)洗(xi)(xi)板(ban)(ban)方(fang)法：吸(xi)去(qu)（不(bu)(bu)可(ke)(ke)觸及(ji)板(ban)(ban)壁）或(huo)(huo)(huo)(huo)(huo)甩掉(diao)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)內(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)體；在(zai)(zai)(zai)實(shi)驗臺上(shang)(shang)鋪墊幾層(ceng)吸(xi)水紙(zhi)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)朝下(xia)(xia)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)力拍幾次(ci)；將(jiang)(jiang)(jiang)(jiang)推(tui)薦(jian)(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌緩沖液(ye)(ye)(ye)(ye)(ye)至少0.3ml注(zhu)(zhu)(zhu)入(ru)孔(kong)(kong)(kong)內(nei)，浸泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。根(gen)(gen)(gen)(gen)據(ju)(ju)(ju)需(xu)(xu)(xu)要(yao)，重(zhong)復(fu)此過(guo)(guo)程(cheng)(cheng)(cheng)(cheng)數(shu)次(ci)。 自(zi)動洗(xi)(xi)板(ban)(ban)：如(ru)(ru)果(guo)有(you)(you)(you)自(zi)動洗(xi)(xi)板(ban)(ban)機，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)在(zai)(zai)(zai)熟練使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)再(zai)(zai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)到(dao)正式實(shi)驗過(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)(zhong)(zhong)。 計(ji)(ji)算(suan)(suan) 以標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃度(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)半對數(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)繪(hui)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)，根(gen)(gen)(gen)(gen)據(ju)(ju)(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)查(cha)出(chu)(chu)(chu)相(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃度(du)(du)(du)(du)(du)(du)；再(zai)(zai)乘(cheng)以稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)；或(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃度(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)計(ji)(ji)算(suan)(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)(xian)回(hui)歸方(fang)程(cheng)(cheng)(cheng)(cheng)式，將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)代入(ru)方(fang)程(cheng)(cheng)(cheng)(cheng)式，計(ji)(ji)算(suan)(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃度(du)(du)(du)(du)(du)(du)，再(zai)(zai)乘(cheng)以稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)，即(ji)(ji)為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際濃度(du)(du)(du)(du)(du)(du)。 注(zhu)(zhu)(zhu)意(yi)事項1.當(dang)混(hun)(hun)(hun)合蛋(dan)白(bai)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)(jin)量(liang)輕(qing)(qing)(qing)緩，避免(mian)起泡(pao)。2.洗(xi)(xi)滌過(guo)(guo)程(cheng)(cheng)(cheng)(cheng)非常重(zhong)要(yao)，不(bu)(bu)充分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌易造成(cheng)(cheng)假陽性(xing)。3.一(yi)次(ci)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)間**控制(zhi)(zhi)(zhi)在(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)量(liang)多(duo)(duo)(duo)，推(tui)薦(jian)(jian)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4.請(qing)(qing)(qing)(qing)每(mei)(mei)(mei)(mei)次(ci)測(ce)(ce)(ce)(ce)(ce)(ce)定的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi)(shi)(shi)(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)，做復(fu)孔(kong)(kong)(kong)。5.如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)待測(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)過(guo)(guo)高(gao)，請(qing)(qing)(qing)(qing)先稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)再(zai)(zai)測(ce)(ce)(ce)(ce)(ce)(ce)定，計(ji)(ji)算(suan)(suan)時(shi)(shi)(shi)(shi)(shi)(shi)請(qing)(qing)(qing)(qing)乘(cheng)以稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)。6.在(zai)(zai)(zai)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)工(gong)(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)(qing)(qing)(qing)以相(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，不(bu)(bu)能(neng)(neng)混(hun)(hun)(hun)淆。7.底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)(qing)(qing)避光保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。 8.不(bu)(bu)要(yao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)其它生產(chan)廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)替換試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)。 注(zhu)(zhu)(zhu)意(yi)事項：收集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)前(qian)(qian)(qian)(qian)必須清(qing)楚要(yao)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)份是(shi)否足夠穩(wen)定。對收集(ji)后(hou)(hou)(hou)當(dang)天(tian)進(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)，儲存(cun)(cun)(cun)(cun)在(zai)(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)有(you)(you)(you)特(te)殊(shu)原因需(xu)(xu)(xu)要(yao)周期(qi)收集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)， 將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)及(ji)時(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)后(hou)(hou)(hou)放(fang)(fang)在(zai)(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)(huo)-70 ℃條件(jian)下(xia)(xia)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。避免(mian)反(fan)(fan)復(fu)凍(dong)融(rong)(rong)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)48 小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)1 個(ge)月(yue)(yue)。-70度(du)(du)(du)(du)(du)(du)可(ke)(ke)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)6 個(ge)月(yue)(yue)。部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)激素(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)需(xu)(xu)(xu)添加(jia)(jia)(jia)(jia)(jia)抑肽(tai)酶(mei)(mei)(mei)(mei)。計(ji)(ji)算(suan)(suan)：以標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃度(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)繪(hui)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)，根(gen)(gen)(gen)(gen)據(ju)(ju)(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)查(cha)出(chu)(chu)(chu)相(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃度(du)(du)(du)(du)(du)(du)；再(zai)(zai)乘(cheng)以稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)；或(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃度(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)計(ji)(ji)算(suan)(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)(xian)回(hui)歸方(fang)程(cheng)(cheng)(cheng)(cheng)式，將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)代入(ru)方(fang)程(cheng)(cheng)(cheng)(cheng)式，計(ji)(ji)算(suan)(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃度(du)(du)(du)(du)(du)(du)，再(zai)(zai)乘(cheng)以稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)，即(ji)(ji)為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際濃度(du)(du)(du)(du)(du)(du)。 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)多(duo)(duo)(duo)糖(Pol)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)性(xing)能(neng)(neng)1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)性(xing)回(hui)歸與(yu)預(yu)期(qi)濃度(du)(du)(du)(du)(du)(du)相(xiang)關(guan)(guan)系(xi)數(shu)R值(zhi)(zhi)為(wei)(wei)(wei)(wei)0.92以上(shang)(shang)。2.批(pi)內(nei)與(yu)批(pi)見應(ying)(ying)(ying)(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)小(xiao)(xiao)于(yu)(yu)(yu)9%和(he)(he)15%
植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)磷(lin)脂(zhi)(zhi)酸(suan)（PA）酶(mei)聯(lian)免(mian)疫吸(xi)附(fu)(fu)測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)僅供(gong)體外(wai)研(yan)(yan)(yan)究使(shi)用(yong)(yong)，不(bu)(bu)用(yong)(yong)于(yu)(yu)臨床診(zhen)斷 使(shi)用(yong)(yong)說(shuo)(shuo)(shuo) 明(ming)(ming)(ming)書 l 本(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)用(yong)(yong)于(yu)(yu)植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)萃取(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)或(huo)其(qi)它(ta)相(xiang)關(guan)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)磷(lin)脂(zhi)(zhi)酸(suan)（PA）的(de)(de)(de)(de)(de)(de)(de)(de)含量。l 有(you)(you)(you)(you)效期：6個(ge)(ge)(ge)月(yue)l 保(bao)(bao)(bao)存(cun)(cun)(cun)條(tiao)(tiao)件(jian)(jian)：2-8℃試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)組(zu)(zu)(zu)成(cheng)(cheng)(cheng)(cheng)：名稱96孔(kong)配置(zhi)(zhi)(zhi)48孔(kong)配置(zhi)(zhi)(zhi)備注微孔(kong)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)(ban)12孔(kong)×8條(tiao)(tiao)12孔(kong)×4條(tiao)(tiao)無(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)0.3mL*6管(guan)0.3mL*6管(guan)無(wu)(wu)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)稀(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)抗體-HRP10mL5mL無(wu)(wu)20×洗(xi)(xi)滌(di)(di)緩沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)25mL15mL按說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書進(jin)行(xing)(xing)稀(xi)(xi)釋(shi)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)A6mL3mL無(wu)(wu)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)B6mL3mL無(wu)(wu)終(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)封板(ban)(ban)(ban)(ban)(ban)(ban)膜2張(zhang)2張(zhang)無(wu)(wu)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書1份1份無(wu)(wu)自封袋1個(ge)(ge)(ge)1個(ge)(ge)(ge)無(wu)(wu)備注：1. （96T/48T）打開包(bao)(bao)裝(zhuang)后請(qing)(qing)及(ji)(ji)時(shi)(shi)(shi)檢(jian)(jian)(jian)(jian)(jian)查所有(you)(you)(you)(you)物(wu)(wu)(wu)(wu)(wu)(wu)品(pin)(pin)(pin)是否齊全(quan)。2. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)依(yi)次(ci)(ci)(ci)為(wei)(wei)：100、50、25、12.5、6.25、0 nmol/L3. 經(jing)過(guo)大(da)量正(zheng)(zheng)常(chang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)驗(yan)(yan)(yan)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)正(zheng)(zheng)常(chang)濃(nong)(nong)(nong)(nong)度(du)(du)值(zhi)(zhi)(zhi)均在(zai)(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)提(ti)供(gong)的(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)范(fan)(fan)圍內(nei)，實(shi)驗(yan)(yan)(yan)過(guo)程中(zhong)(zhong)(zhong)(zhong)直(zhi)(zhi)接取(qu)(qu)50μL樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)上(shang)(shang)(shang)樣(yang)(yang)(yang)(yang)(yang)(yang)即可(ke)(ke)(ke)。當(dang)(dang)有(you)(you)(you)(you)部(bu)分樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)值(zhi)(zhi)(zhi)超過(guo)＊大(da)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)時(shi)(shi)(shi)，可(ke)(ke)(ke)用(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)稀(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)(ye)(ye)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)進(jin)行(xing)(xing)適(shi)當(dang)(dang)稀(xi)(xi)釋(shi)后再(zai)進(jin)行(xing)(xing)實(shi)驗(yan)(yan)(yan)。檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)前(qian)(qian)準(zhun)(zhun)(zhun)(zhun)備工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)：1. 請(qing)(qing)提(ti)前(qian)(qian)20分鐘從冰(bing)箱中(zhong)(zhong)(zhong)(zhong)取(qu)(qu)出(chu)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)，平(ping)衡(heng)至(zhi)室(shi)(shi)溫(wen)(wen)。2. 從冰(bing)箱中(zhong)(zhong)(zhong)(zhong)取(qu)(qu)出(chu)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)縮洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)可(ke)(ke)(ke)能(neng)(neng)(neng)有(you)(you)(you)(you)結(jie)(jie)(jie)晶，屬于(yu)(yu)正(zheng)(zheng)常(chang)現象；放置(zhi)(zhi)(zhi)室(shi)(shi)溫(wen)(wen)，輕搖均勻(yun)(yun)(yun)(yun)(yun)，待結(jie)(jie)(jie)晶完(wan)全(quan)溶(rong)(rong)解后再(zai)配置(zhi)(zhi)(zhi)洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)。可(ke)(ke)(ke)將(jiang)20ml濃(nong)(nong)(nong)(nong)縮洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)用(yong)(yong)蒸(zheng)餾水(shui)(shui)(shui)(shui)(shui)或(huo)去(qu)離子水(shui)(shui)(shui)(shui)(shui)稀(xi)(xi)釋(shi)配置(zhi)(zhi)(zhi)成(cheng)(cheng)(cheng)(cheng)400ml工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)濃(nong)(nong)(nong)(nong)度(du)(du)的(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)，未用(yong)(yong)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)放回(hui)4℃3. 20×洗(xi)(xi)滌(di)(di)緩沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)釋(shi)：蒸(zheng)餾水(shui)(shui)(shui)(shui)(shui)按1：20稀(xi)(xi)釋(shi)，即1份20×洗(xi)(xi)滌(di)(di)緩沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)加(jia)(jia)19份蒸(zheng)餾水(shui)(shui)(shui)(shui)(shui)。實(shi)驗(yan)(yan)(yan)原理(li)(li)：試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)采用(yong)(yong)雙(shuang)抗體一(yi)(yi)步夾(jia)心(xin)(xin)(xin)法(fa)酶(mei)聯(lian)免(mian)疫吸(xi)附(fu)(fu)試(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)（ELISA）。往預先(xian)包(bao)(bao)被植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)磷(lin)脂(zhi)(zhi)酸(suan)（PA）捕獲(huo)抗體的(de)(de)(de)(de)(de)(de)(de)(de)包(bao)(bao)被微孔(kong)中(zhong)(zhong)(zhong)(zhong)，依(yi)次(ci)(ci)(ci)加(jia)(jia)入(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)抗體，經(jing)過(guo)溫(wen)(wen)育(yu)并(bing)(bing)徹底(di)(di)洗(xi)(xi)滌(di)(di)。用(yong)(yong)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)TMB顯色(se)(se)(se)，TMB在(zai)(zai)(zai)(zai)過(guo)氧(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)的(de)(de)(de)(de)(de)(de)(de)(de)催(cui)化(hua)下轉(zhuan)化(hua)成(cheng)(cheng)(cheng)(cheng)藍色(se)(se)(se)，并(bing)(bing)在(zai)(zai)(zai)(zai)酸(suan)的(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)下轉(zhuan)化(hua)成(cheng)(cheng)(cheng)(cheng)＊終(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)(se)(se)。顏色(se)(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)磷(lin)脂(zhi)(zhi)酸(suan)（PA）呈正(zheng)(zheng)相(xiang)關(guan)。用(yong)(yong)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)(zai)(zai)450nm 波(bo)長下測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)吸(xi)光度(du)(du)（OD 值(zhi)(zhi)(zhi)），計(ji)算(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)含量。實(shi)驗(yan)(yan)(yan)所需(xu)自備試(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)器材：1. 酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀（450nm）2. 高精(jing)度(du)(du)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)器及(ji)(ji)槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒(heng)溫(wen)(wen)箱4. 蒸(zheng)餾水(shui)(shui)(shui)(shui)(shui)或(huo)去(qu)離子水(shui)(shui)(shui)(shui)(shui)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)處理(li)(li)及(ji)(ji)要(yao)求：植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)組(zu)(zu)(zu)織樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)ELISA檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)處理(li)(li)方法(fa)一(yi)(yi)、組(zu)(zu)(zu)織勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)的(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)備： 1、取(qu)(qu)組(zu)(zu)(zu)織塊（0.1g-0.5g）＊少(shao)可(ke)(ke)(ke)到(dao)(dao)5-10mg在(zai)(zai)(zai)(zai)冰(bing)冷(leng)的(de)(de)(de)(de)(de)(de)(de)(de)PBS中(zhong)(zhong)(zhong)(zhong)漂(piao)(piao)洗(xi)(xi)，濾(lv)紙拭(shi)干，準(zhun)(zhun)(zhun)(zhun)確(que)稱重(zhong)，放入(ru)(ru)(ru)(ru)5ml的(de)(de)(de)(de)(de)(de)(de)(de)勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)管(guan)中(zhong)(zhong)(zhong)(zhong)。 2、按重(zhong)量(g):體積(ml)=1:9的(de)(de)(de)(de)(de)(de)(de)(de)比例(li)加(jia)(jia)入(ru)(ru)(ru)(ru)9倍體積的(de)(de)(de)(de)(de)(de)(de)(de)勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)介質(zhi)(zhi)于(yu)(yu)勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)管(guan)中(zhong)(zhong)(zhong)(zhong)，冰(bing)水(shui)(shui)(shui)(shui)(shui)浴條(tiao)(tiao)件(jian)(jian)下,用(yong)(yong)眼科小剪(jian)盡快剪(jian)碎組(zu)(zu)(zu)織塊。 3、勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)的(de)(de)(de)(de)(de)(de)(de)(de)方式(shi)：手(shou)工(gong)(gong)(gong)勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)，機器勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)。 ① 手(shou)工(gong)(gong)(gong)勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)：左(zuo)手(shou)持勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)管(guan)將(jiang)下端插(cha)入(ru)(ru)(ru)(ru)盛(sheng)有(you)(you)(you)(you)冰(bing)水(shui)(shui)(shui)(shui)(shui)混(hun)合(he)物(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)器皿中(zhong)(zhong)(zhong)(zhong)，右手(shou)將(jiang)搗桿垂直(zhi)(zhi)插(cha)入(ru)(ru)(ru)(ru)套管(guan)中(zhong)(zhong)(zhong)(zhong)，上(shang)(shang)(shang)下轉(zhuan)動研(yan)(yan)(yan)磨數十次(ci)(ci)(ci)（6-8分鐘），充分研(yan)(yan)(yan)碎，制(zhi)成(cheng)(cheng)(cheng)(cheng)10%的(de)(de)(de)(de)(de)(de)(de)(de)勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)液(ye)(ye)(ye)(ye)(ye)(ye)。 ② 機器勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)：用(yong)(yong)組(zu)(zu)(zu)織搗碎機10000-15000 轉(zhuan)/分上(shang)(shang)(shang)下研(yan)(yan)(yan)磨制(zhi)成(cheng)(cheng)(cheng)(cheng)10%組(zu)(zu)(zu)織勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)，也可(ke)(ke)(ke)用(yong)(yong)內(nei)切(qie)式(shi)組(zu)(zu)(zu)織勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)機制(zhi)備（勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)時(shi)(shi)(shi)間(jian)(jian)10秒/次(ci)(ci)(ci)，間(jian)(jian)隙30秒，連續3-5次(ci)(ci)(ci)，在(zai)(zai)(zai)(zai)冰(bing)水(shui)(shui)(shui)(shui)(shui)中(zhong)(zhong)(zhong)(zhong)進(jin)行(xing)(xing),可(ke)(ke)(ke)適(shi)當(dang)(dang)延(yan)長勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)時(shi)(shi)(shi)間(jian)(jian)），鏡檢(jian)(jian)(jian)(jian)(jian)觀察(cha)。4、將(jiang)制(zhi)備好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)10%勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)液(ye)(ye)(ye)(ye)(ye)(ye)用(yong)(yong)普(pu)通離心(xin)(xin)(xin)機或(huo)低(di)(di)溫(wen)(wen)低(di)(di)速(su)離心(xin)(xin)(xin)機3000轉(zhuan)/分左(zuo)右,離心(xin)(xin)(xin)10-15分鐘，取(qu)(qu)上(shang)(shang)(shang)清液(ye)(ye)(ye)(ye)(ye)(ye)進(jin)行(xing)(xing)測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)。二、勻(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)(jiang)介質(zhi)(zhi)： 一(yi)(yi)般采用(yong)(yong)0.05 mol/L Tris-HCl, pH 7.4 磷(lin)酸(suan)鹽(yan)緩沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)(PBS),客戶(hu)可(ke)(ke)(ke)根(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)及(ji)(ji)測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)指(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)的(de)(de)(de)(de)(de)(de)(de)(de)情況自行(xing)(xing)設(she)定(ding)(ding)(ding)濃(nong)(nong)(nong)(nong)度(du)(du),目的(de)(de)(de)(de)(de)(de)(de)(de)是保(bao)(bao)(bao)持樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)等(deng)滲環(huan)境。三(san)、樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)保(bao)(bao)(bao)存(cun)(cun)(cun)： 1. 植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)組(zu)(zu)(zu)織樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)暫時(shi)(shi)(shi)不(bu)(bu)測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，可(ke)(ke)(ke)立即低(di)(di)溫(wen)(wen)凍(dong)存(cun)(cun)(cun)，溫(wen)(wen)度(du)(du)越低(di)(di)越好(hao)，中(zhong)(zhong)(zhong)(zhong)間(jian)(jian)如(ru)(ru)不(bu)(bu)反(fan)復凍(dong)融，-20℃以(yi)(yi)下可(ke)(ke)(ke)保(bao)(bao)(bao) 存(cun)(cun)(cun)三(san)個(ge)(ge)(ge)月(yue),-70℃以(yi)(yi)下可(ke)(ke)(ke)保(bao)(bao)(bao)存(cun)(cun)(cun)六個(ge)(ge)(ge)月(yue)。注意事項：1. 嚴格按照規(gui)定(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)時(shi)(shi)(shi)間(jian)(jian)和(he)(he)(he)溫(wen)(wen)度(du)(du)進(jin)行(xing)(xing)溫(wen)(wen)育(yu)以(yi)(yi)保(bao)(bao)(bao)證(zheng)(zheng)準(zhun)(zhun)(zhun)(zhun)確(que)結(jie)(jie)(jie)果(guo)。所有(you)(you)(you)(you)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)都必(bi)須(xu)在(zai)(zai)(zai)(zai)使(shi)用(yong)(yong)前(qian)(qian)達到(dao)(dao)室(shi)(shi)溫(wen)(wen)20-25℃。使(shi)用(yong)(yong)后立即冷(leng)藏保(bao)(bao)(bao)存(cun)(cun)(cun)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)。2. 洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)不(bu)(bu)正(zheng)(zheng)確(que)可(ke)(ke)(ke)以(yi)(yi)導致(zhi)(zhi)不(bu)(bu)準(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)結(jie)(jie)(jie)果(guo)。在(zai)(zai)(zai)(zai)加(jia)(jia)入(ru)(ru)(ru)(ru)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)前(qian)(qian)確(que)保(bao)(bao)(bao)盡量吸(xi)干孔(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體。溫(wen)(wen)育(yu)過(guo)程中(zhong)(zhong)(zhong)(zhong)不(bu)(bu)要(yao)讓微孔(kong)干燥(zao)掉。3. 消除板(ban)(ban)(ban)(ban)(ban)(ban)底(di)(di)殘留的(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)體和(he)(he)(he)手(shou)指(zhi)印，否則影(ying)響(xiang)OD值(zhi)(zhi)(zhi)。4. 底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)顯色(se)(se)(se)液(ye)(ye)(ye)(ye)(ye)(ye)應呈無(wu)(wu)色(se)(se)(se)或(huo)很(hen)淺的(de)(de)(de)(de)(de)(de)(de)(de)顏色(se)(se)(se)，已經(jing)變(bian)藍的(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)不(bu)(bu)能(neng)(neng)(neng)使(shi)用(yong)(yong)。5. 避(bi)免(mian)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)和(he)(he)(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)交叉(cha)污染(ran)以(yi)(yi)免(mian)造(zao)成(cheng)(cheng)(cheng)(cheng)錯誤(wu)結(jie)(jie)(jie)果(guo)。6. 在(zai)(zai)(zai)(zai)儲存(cun)(cun)(cun)和(he)(he)(he)溫(wen)(wen)育(yu)時(shi)(shi)(shi)避(bi)免(mian)強光直(zhi)(zhi)接照射。7. 平(ping)衡(heng)至(zhi)室(shi)(shi)溫(wen)(wen)后再(zai)打開密(mi)(mi)封袋以(yi)(yi)防水(shui)(shui)(shui)(shui)(shui)滴凝聚(ju)在(zai)(zai)(zai)(zai)冷(leng)板(ban)(ban)(ban)(ban)(ban)(ban)條(tiao)(tiao)上(shang)(shang)(shang)。8. 任何反(fan)應試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)不(bu)(bu)能(neng)(neng)(neng)接觸漂(piao)(piao)白(bai)溶(rong)(rong)劑(ji)(ji)(ji)(ji)(ji)或(huo)漂(piao)(piao)白(bai)溶(rong)(rong)劑(ji)(ji)(ji)(ji)(ji)所散(san)發的(de)(de)(de)(de)(de)(de)(de)(de)強烈(lie)氣(qi)體。任何漂(piao)(piao)白(bai)成(cheng)(cheng)(cheng)(cheng)分都會破(po)壞試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)反(fan)應試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)生物(wu)(wu)(wu)(wu)(wu)(wu)活性。9. 不(bu)(bu)能(neng)(neng)(neng)使(shi)用(yong)(yong)過(guo)期產(chan)(chan)(chan)品(pin)(pin)(pin)。10. 如(ru)(ru)果(guo)可(ke)(ke)(ke)能(neng)(neng)(neng)傳播疾病，所有(you)(you)(you)(you)的(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)都應管(guan)理(li)(li)好(hao)，按照規(gui)定(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)程序處理(li)(li)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)和(he)(he)(he)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)裝(zhuang)置(zhi)(zhi)(zhi)。操(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)步驟(zou)：1．從室(shi)(shi)溫(wen)(wen)平(ping)衡(heng)60min后的(de)(de)(de)(de)(de)(de)(de)(de)鋁箔袋中(zhong)(zhong)(zhong)(zhong)取(qu)(qu)出(chu)所需(xu)板(ban)(ban)(ban)(ban)(ban)(ban)條(tiao)(tiao)，剩余板(ban)(ban)(ban)(ban)(ban)(ban)條(tiao)(tiao)用(yong)(yong)自封袋密(mi)(mi)封放回(hui)4℃。2．設(she)置(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)和(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)孔(kong)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)各加(jia)(jia)不(bu)(bu)同(tong)濃(nong)(nong)(nong)(nong)度(du)(du)的(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)50μL。3．樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)孔(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)入(ru)(ru)(ru)(ru)待測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)50μL；空(kong)白(bai)孔(kong)不(bu)(bu)加(jia)(jia)。樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)不(bu)(bu)足，或(huo)者(zhe)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)濃(nong)(nong)(nong)(nong)度(du)(du)過(guo)高，可(ke)(ke)(ke)用(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)稀(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)(ye)(ye)做稀(xi)(xi)釋(shi)。4. 除空(kong)白(bai)孔(kong)外(wai)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)和(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)孔(kong)中(zhong)(zhong)(zhong)(zhong)每孔(kong)加(jia)(jia)入(ru)(ru)(ru)(ru)辣根(gen)(gen)過(guo)氧(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)（HRP）標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)抗體100μL，用(yong)(yong)封板(ban)(ban)(ban)(ban)(ban)(ban)膜封住(zhu)反(fan)應孔(kong)，37℃水(shui)(shui)(shui)(shui)(shui)浴鍋或(huo)恒(heng)溫(wen)(wen)箱溫(wen)(wen)育(yu)60min。5．棄去(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體，吸(xi)水(shui)(shui)(shui)(shui)(shui)紙上(shang)(shang)(shang)拍干，每孔(kong)加(jia)(jia)滿洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)（350μL），靜(jing)置(zhi)(zhi)(zhi)1min，甩去(qu)洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)，吸(xi)水(shui)(shui)(shui)(shui)(shui)紙上(shang)(shang)(shang)拍干，如(ru)(ru)此重(zhong)復洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)5次(ci)(ci)(ci)（也可(ke)(ke)(ke)用(yong)(yong)洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)機洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)）。6. 每孔(kong)加(jia)(jia)入(ru)(ru)(ru)(ru)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)A、B各50μL，37℃避(bi)光孵(fu)育(yu)15min。7. 每孔(kong)加(jia)(jia)入(ru)(ru)(ru)(ru)終(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)50μL，15min內(nei)，在(zai)(zai)(zai)(zai)450nm波(bo)長處測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)各孔(kong)的(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)。實(shi)驗(yan)(yan)(yan)結(jie)(jie)(jie)果(guo)計(ji)算(suan)：繪(hui)制(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲線(xian)：以(yi)(yi)所測(ce)(ce)(ce)(ce)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)為(wei)(wei)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)值(zhi)(zhi)(zhi)為(wei)(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)或(huo)用(yong)(yong)相(xiang)關(guan)軟件(jian)(jian)繪(hui)制(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲線(xian)，并(bing)(bing)得到(dao)(dao)直(zhi)(zhi)線(xian)回(hui)歸(gui)方程，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)(ru)(ru)(ru)方程，計(ji)算(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)。 （此圖僅供(gong)參(can)考(kao)）試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)性能(neng)(neng)(neng)：1. 檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)范(fan)(fan)圍：1.95 nmol/L –100 nmol/L。2. 靈敏度(du)(du)：＊低(di)(di)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)濃(nong)(nong)(nong)(nong)度(du)(du)小于(yu)(yu)0.78 nmol/L。3. 特(te)異性：不(bu)(bu)與其(qi)它(ta)可(ke)(ke)(ke)溶(rong)(rong)性結(jie)(jie)(jie)構(gou)類似物(wu)(wu)(wu)(wu)(wu)(wu)交叉(cha)反(fan)應。4. 重(zhong)復性：板(ban)(ban)(ban)(ban)(ban)(ban)內(nei)變(bian)異系數小于(yu)(yu)10% ，板(ban)(ban)(ban)(ban)(ban)(ban)間(jian)(jian)變(bian)異系數小于(yu)(yu)15% 。技術(shu)小提(ti)示：1. 當(dang)(dang)混(hun)合(he)或(huo)重(zhong)溶(rong)(rong)蛋白(bai)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)，盡量避(bi)免(mian)起沫。2. 為(wei)(wei)了避(bi)免(mian)交叉(cha)感染(ran)，配置(zhi)(zhi)(zhi)不(bu)(bu)同(tong)濃(nong)(nong)(nong)(nong)度(du)(du)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)、上(shang)(shang)(shang)樣(yang)(yang)(yang)(yang)(yang)(yang)、加(jia)(jia)不(bu)(bu)同(tong)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)都需(xu)要(yao)更換槍頭。另(ling)外(wai)不(bu)(bu)同(tong)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)請(qing)(qing)分別使(shi)用(yong)(yong)不(bu)(bu)同(tong)的(de)(de)(de)(de)(de)(de)(de)(de)移液(ye)(ye)(ye)(ye)(ye)(ye)槽。3. 每次(ci)(ci)(ci)孵(fu)育(yu)時(shi)(shi)(shi)，請(qing)(qing)正(zheng)(zheng)確(que)使(shi)用(yong)(yong)封板(ban)(ban)(ban)(ban)(ban)(ban)膠可(ke)(ke)(ke)保(bao)(bao)(bao)證(zheng)(zheng)結(jie)(jie)(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)確(que)性。4. 混(hun)合(he)后的(de)(de)(de)(de)(de)(de)(de)(de)顯色(se)(se)(se)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)在(zai)(zai)(zai)(zai)上(shang)(shang)(shang)板(ban)(ban)(ban)(ban)(ban)(ban)前(qian)(qian)應為(wei)(wei)無(wu)(wu)色(se)(se)(se)，請(qing)(qing)避(bi)光保(bao)(bao)(bao)存(cun)(cun)(cun)；加(jia)(jia)入(ru)(ru)(ru)(ru)微孔(kong)板(ban)(ban)(ban)(ban)(ban)(ban)后，將(jiang)由(you)無(wu)(wu)色(se)(se)(se)變(bian)成(cheng)(cheng)(cheng)(cheng)不(bu)(bu)同(tong)深度(du)(du)的(de)(de)(de)(de)(de)(de)(de)(de)藍色(se)(se)(se)。5. 終(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)上(shang)(shang)(shang)板(ban)(ban)(ban)(ban)(ban)(ban)順序應同(tong)顯色(se)(se)(se)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)板(ban)(ban)(ban)(ban)(ban)(ban)順序一(yi)(yi)致(zhi)(zhi)；加(jia)(jia)入(ru)(ru)(ru)(ru)終(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)后，孔(kong)內(nei)顏色(se)(se)(se)由(you)藍變(bian)黃；若孔(kong)內(nei)有(you)(you)(you)(you)綠色(se)(se)(se)，則表明(ming)(ming)(ming)孔(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體未混(hun)勻(yun)(yun)(yun)(yun)(yun)；請(qing)(qing)充分混(hun)合(he)。說(shuo)(shuo)(shuo)明(ming)(ming)(ming)：1. 由(you)于(yu)(yu)現有(you)(you)(you)(you)條(tiao)(tiao)件(jian)(jian)及(ji)(ji)科學技術(shu)水(shui)(shui)(shui)(shui)(shui)平(ping)尚不(bu)(bu)能(neng)(neng)(neng)對(dui)所有(you)(you)(you)(you)供(gong)貨商(shang)提(ti)供(gong)的(de)(de)(de)(de)(de)(de)(de)(de)所有(you)(you)(you)(you)原料(liao)進(jin)行(xing)(xing)全(quan)面的(de)(de)(de)(de)(de)(de)(de)(de)鑒定(ding)(ding)(ding)與分析，本(ben)(ben)(ben)(ben)(ben)(ben)(ben)產(chan)(chan)(chan)品(pin)(pin)(pin)可(ke)(ke)(ke)能(neng)(neng)(neng)存(cun)(cun)(cun)在(zai)(zai)(zai)(zai)一(yi)(yi)定(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)量技術(shu)風險。2. ＊終(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗(yan)(yan)(yan)結(jie)(jie)(jie)果(guo)與試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)(you)(you)效性、實(shi)驗(yan)(yan)(yan)者(zhe)的(de)(de)(de)(de)(de)(de)(de)(de)相(xiang)關(guan)操(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)以(yi)(yi)及(ji)(ji)當(dang)(dang)時(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗(yan)(yan)(yan)環(huan)境密(mi)(mi)切(qie)相(xiang)關(guan)，請(qing)(qing)務必(bi)準(zhun)(zhun)(zhun)(zhun)備充足的(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)備份。3. 不(bu)(bu)同(tong)批次(ci)(ci)(ci)的(de)(de)(de)(de)(de)(de)(de)(de)同(tong)一(yi)(yi)產(chan)(chan)(chan)品(pin)(pin)(pin)可(ke)(ke)(ke)能(neng)(neng)(neng)會有(you)(you)(you)(you)少(shao)許差別，如(ru)(ru)：檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)限、靈敏度(du)(du)以(yi)(yi)及(ji)(ji)顯色(se)(se)(se)時(shi)(shi)(shi)間(jian)(jian)等(deng)，請(qing)(qing)依(yi)據(ju)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)內(nei)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書進(jin)行(xing)(xing)實(shi)驗(yan)(yan)(yan)操(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)，網站電子版(ban)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書僅作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)參(can)考(kao)。4. 只有(you)(you)(you)(you)全(quan)部(bu)使(shi)用(yong)(yong)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)配套試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)才能(neng)(neng)(neng)保(bao)(bao)(bao)證(zheng)(zheng)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)效果(guo)，不(bu)(bu)能(neng)(neng)(neng)混(hun)用(yong)(yong)其(qi)他制(zhi)造(zao)商(shang)的(de)(de)(de)(de)(de)(de)(de)(de)產(chan)(chan)(chan)品(pin)(pin)(pin)。只有(you)(you)(you)(you)嚴格遵(zun)守本(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗(yan)(yan)(yan)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)才會得到(dao)(dao)＊佳的(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)結(jie)(jie)(jie)果(guo)。
實(shi)驗(yan)(yan)(yan)(yan)原(yuan)(yuan)(yuan)理 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)雙抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)一(yi)(yi)步夾心(xin)法酶(mei)(mei)(mei)聯免(mian)(mian)疫(yi)吸附試(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)（ELISA）。往預(yu)先(xian)(xian)包被(bei)硝(xiao)(xiao)酸(suan)(suan)(suan)(suan)還(huan)(huan)原(yuan)(yuan)(yuan)酶(mei)(mei)(mei)(NR)捕獲抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被(bei)微孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，依(yi)次(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)，經(jing)過(guo)(guo)(guo)(guo)溫(wen)育(yu)并(bing)徹(che)底(di)(di)(di)洗(xi)(xi)(xi)滌(di)(di)。用(yong)(yong)(yong)(yong)(yong)(yong)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)TMB顯(xian)色，TMB在(zai)(zai)(zai)(zai)(zai)(zai)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)(hua)(hua)下(xia)(xia)(xia)轉化(hua)(hua)(hua)(hua)成藍(lan)色，并(bing)在(zai)(zai)(zai)(zai)(zai)(zai)酸(suan)(suan)(suan)(suan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)下(xia)(xia)(xia)轉化(hua)(hua)(hua)(hua)成＊終(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色。顏色的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺(qian)和(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)硝(xiao)(xiao)酸(suan)(suan)(suan)(suan)還(huan)(huan)原(yuan)(yuan)(yuan)酶(mei)(mei)(mei)(NR)呈正(zheng)相關(guan)(guan)。用(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)(zai)(zai)(zai)(zai)450nm 波長下(xia)(xia)(xia)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)吸光度(du)(du)(du)（OD 值(zhi)(zhi)(zhi)），計算(suan)(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)。保(bao)存(cun)條件(jian)及有(you)(you)效(xiao)期1.本(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)體(ti)(ti)(ti)(ti)(ti)外定(ding)(ding)量(liang)(liang)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)血(xue)(xue)清(qing)(qing)、血(xue)(xue)漿、組織勻(yun)(yun)(yun)(yun)漿及相關(guan)(guan)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)中(zhong)(zhong)硝(xiao)(xiao)酸(suan)(suan)(suan)(suan)還(huan)(huan)原(yuan)(yuan)(yuan)酶(mei)(mei)(mei)(NR)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)量(liang)(liang)；2.試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)保(bao)存(cun)：2-8℃；3.有(you)(you)效(xiao)期：6個月。植(zhi)物(wu)(wu)(wu)(wu)(wu)硝(xiao)(xiao)酸(suan)(suan)(suan)(suan)還(huan)(huan)原(yuan)(yuan)(yuan)酶(mei)(mei)(mei)(NR)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集(ji)及保(bao)存(cun)1.血(xue)(xue)清(qing)(qing)：全血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)請(qing)于(yu)室溫(wen)放(fang)置(zhi)2小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)(huo)(huo)(huo)4℃過(guo)(guo)(guo)(guo)一(yi)(yi)晚后(hou)(hou)(hou)(hou)于(yu)1000 x g離(li)心(xin)20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，取(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)即可(ke)(ke)(ke)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)放(fang)于(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)存(cun)，但應(ying)(ying)(ying)避(bi)免(mian)(mian)反(fan)復(fu)(fu)凍融。 2.血(xue)(xue)漿：可(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)(huo)(huo)肝素(su)(su)(su)(su)(su)(su)(su)作(zuo)(zuo)為(wei)(wei)抗(kang)(kang)(kang)(kang)(kang)凝劑(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)采(cai)集(ji)后(hou)(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)于(yu)2 - 8° C 1000 x g離(li)心(xin)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)放(fang)于(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)存(cun)，但應(ying)(ying)(ying)避(bi)免(mian)(mian)反(fan)復(fu)(fu)凍融。 3.細胞培養物(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)清(qing)(qing)或(huo)(huo)(huo)(huo)(huo)(huo)其它生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)：1000 x g離(li)心(xin)20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，取(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)即可(ke)(ke)(ke)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)放(fang)于(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)存(cun)，但應(ying)(ying)(ying)避(bi)免(mian)(mian)反(fan)復(fu)(fu)凍融。 (注(zhu)(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)溶(rong)(rong)(rong)血(xue)(xue)會影(ying)響**檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)結果(guo)，因(yin)此(ci)(ci)溶(rong)(rong)(rong)血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)不(bu)宜進(jin)行此(ci)(ci)項(xiang)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)。) 植(zhi)物(wu)(wu)(wu)(wu)(wu)硝(xiao)(xiao)酸(suan)(suan)(suan)(suan)還(huan)(huan)原(yuan)(yuan)(yuan)酶(mei)(mei)(mei)(NR)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he) 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)(yuan)則(ze)：首先(xian)(xian)通(tong)過(guo)(guo)(guo)(guo)文獻檢(jian)(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)(fang)(fang)式(shi)(shi)了解待(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致(zhi)含(han)量(liang)(liang)，確定(ding)(ding)適(shi)當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)。只有(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范(fan)圍(wei)內(nei)(nei)，檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結果(guo)才(cai)是(shi)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)中(zhong)(zhong)，應(ying)(ying)(ying)做(zuo)好(hao)(hao)(hao)詳(xiang)細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記錄(lu)。計算(suan)(suan)(suan)濃(nong)(nong)(nong)(nong)度(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)了“N”倍，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)應(ying)(ying)(ying)再(zai)(zai)(zai)乘(cheng)以(yi)“N”。 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)(yuan)則(ze)：2瓶，每(mei)(mei)(mei)瓶臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)1ml，蓋好(hao)(hao)(hao)后(hou)(hou)(hou)(hou)靜置(zhi)10分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)上(shang)(shang)(shang)，然后(hou)(hou)(hou)(hou)反(fan)復(fu)(fu)顛倒/搓動以(yi)助溶(rong)(rong)(rong)解，其濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)300 pg/ml，做(zuo)系(xi)列倍比(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)直接作(zuo)(zuo)為(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)度(du)(du)(du)0 pg/ml，臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 如(ru)(ru)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取(qu)(qu)0.5ml（不(bu)要(yao)(yao)少(shao)于(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)含(han)0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)中(zhong)(zhong)，混勻(yun)(yun)(yun)(yun)即可(ke)(ke)(ke)，其余濃(nong)(nong)(nong)(nong)度(du)(du)(du)以(yi)此(ci)(ci)類推(tui)。 生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)(yuan)則(ze)：臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)根(gen)據(ju)預(yu)先(xian)(xian)計算(suan)(suan)(suan)好(hao)(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)驗(yan)(yan)(yan)(yan)所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際(ji)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕輕混勻(yun)(yun)(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 辣(la)(la)(la)根(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記親(qin)(qin)和(he)(he)素(su)(su)(su)(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)(yuan)則(ze)：臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)辣(la)(la)(la)根(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記親(qin)(qin)和(he)(he)素(su)(su)(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)根(gen)據(ju)預(yu)先(xian)(xian)計算(suan)(suan)(suan)好(hao)(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)驗(yan)(yan)(yan)(yan)所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際(ji)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl辣(la)(la)(la)根(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記親(qin)(qin)和(he)(he)素(su)(su)(su)(su)(su)(su)(su)加(jia)(jia)(jia)(jia)(jia)990μl辣(la)(la)(la)根(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記親(qin)(qin)和(he)(he)素(su)(su)(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕輕混勻(yun)(yun)(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 植(zhi)物(wu)(wu)(wu)(wu)(wu)硝(xiao)(xiao)酸(suan)(suan)(suan)(suan)還(huan)(huan)原(yuan)(yuan)(yuan)酶(mei)(mei)(mei)(NR)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)操作(zuo)(zuo)步驟 實(shi)驗(yan)(yan)(yan)(yan)開始前(qian)(qian)，請(qing)提前(qian)(qian)配(pei)(pei)(pei)(pei)(pei)(pei)置(zhi)好(hao)(hao)(hao)所有(you)(you)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)或(huo)(huo)(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)，均需(xu)混勻(yun)(yun)(yun)(yun)，混勻(yun)(yun)(yun)(yun)時(shi)(shi)(shi)(shi)(shi)盡(jin)(jin)量(liang)(liang)避(bi)免(mian)(mian)起(qi)泡(pao)(pao)。每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)該做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)。如(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)過(guo)(guo)(guo)(guo)高時(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)進(jin)行稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)使(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合(he)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)范(fan)圍(wei)。 1.加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)別(bie)設空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)(kong)、待(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)。空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)(huo)(huo)待(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)(zhu)意不(bu)要(yao)(yao)有(you)(you)氣泡(pao)(pao)，加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)于(yu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)底(di)(di)(di)部(bu)，盡(jin)(jin)量(liang)(liang)不(bu)觸(chu)及孔(kong)(kong)(kong)(kong)(kong)(kong)壁，輕輕晃動混勻(yun)(yun)(yun)(yun)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)蓋或(huo)(huo)(huo)(huo)(huo)(huo)覆膜，37℃反(fan)應(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。 為(wei)(wei)保(bao)證實(shi)驗(yan)(yan)(yan)(yan)結果(guo)有(you)(you)效(xiao)性(xing)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)驗(yan)(yan)(yan)(yan)請(qing)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)新(xin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 2.棄(qi)去(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩干(gan)(gan)，不(bu)用(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)(xi)滌(di)(di)。每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取(qu)(qu)1μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕輕混勻(yun)(yun)(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。 3.溫(wen)育(yu)60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)，棄(qi)去(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩干(gan)(gan)，洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)3次(ci)(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)浸泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)，甩干(gan)(gan)。4.每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)辣(la)(la)(la)根(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記親(qin)(qin)和(he)(he)素(su)(su)(su)(su)(su)(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。5.溫(wen)育(yu)60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)，棄(qi)去(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩干(gan)(gan)，洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)5次(ci)(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)浸泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)，甩干(gan)(gan)。6.依(yi)序(xu)(xu)每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)光顯(xian)色（30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)，此(ci)(ci)時(shi)(shi)(shi)(shi)(shi)肉眼可(ke)(ke)(ke)見(jian)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)有(you)(you)明顯(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)藍(lan)色，后(hou)(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)梯度(du)(du)(du)不(bu)明顯(xian)，即可(ke)(ke)(ke)終(zhong)(zhong)(zhong)(zhong)止）。7.依(yi)序(xu)(xu)每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)(zhong)(zhong)止溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)(zhong)(zhong)止反(fan)應(ying)(ying)(ying)（此(ci)(ci)時(shi)(shi)(shi)(shi)(shi)藍(lan)色立轉黃色）。終(zhong)(zhong)(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)順(shun)序(xu)(xu)應(ying)(ying)(ying)盡(jin)(jin)量(liang)(liang)與底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)順(shun)序(xu)(xu)相同。為(wei)(wei)了保(bao)證實(shi)驗(yan)(yan)(yan)(yan)結果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)，底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)反(fan)應(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)間到后(hou)(hou)(hou)(hou)應(ying)(ying)(ying)盡(jin)(jin)快(kuai)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)終(zhong)(zhong)(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)聯儀在(zai)(zai)(zai)(zai)(zai)(zai)450nm波長依(yi)序(xu)(xu)測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光密度(du)(du)(du)（OD值(zhi)(zhi)(zhi)）。 在(zai)(zai)(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)內(nei)(nei)進(jin)行檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)。植(zhi)物(wu)(wu)(wu)(wu)(wu)硝(xiao)(xiao)酸(suan)(suan)(suan)(suan)還(huan)(huan)原(yuan)(yuan)(yuan)酶(mei)(mei)(mei)(NR)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)注(zhu)(zhu)意事(shi)項(xiang)1.用(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)在(zai)(zai)(zai)(zai)(zai)(zai)初次(ci)(ci)(ci)(ci)(ci)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)時(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)將(jiang)(jiang)(jiang)(jiang)各(ge)種(zhong)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)管(guan)離(li)心(xin)數(shu)分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，以(yi)便(bian)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)集(ji)中(zhong)(zhong)到管(guan)底(di)(di)(di)。2.每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)驗(yan)(yan)(yan)(yan)留一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)為(wei)(wei)空(kong)白(bai)調零孔(kong)(kong)(kong)(kong)(kong)(kong)，該孔(kong)(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)(jia)(jia)(jia)任(ren)何試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)，只是(shi)**加(jia)(jia)(jia)(jia)(jia)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)及2N H2SO4。測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)時(shi)(shi)(shi)(shi)(shi)先(xian)(xian)用(yong)(yong)(yong)(yong)(yong)(yong)此(ci)(ci)孔(kong)(kong)(kong)(kong)(kong)(kong)調OD值(zhi)(zhi)(zhi)至(zhi)零。 3.為(wei)(wei)防止樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)時(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)(jiang)(jiang)反(fan)應(ying)(ying)(ying)板(ban)(ban)(ban)(ban)放(fang)于(yu)鋪有(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)(he)內(nei)(nei)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)蓋或(huo)(huo)(huo)(huo)(huo)(huo)覆膜。 4.未使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)或(huo)(huo)(huo)(huo)(huo)(huo)者試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)，請(qing)于(yu)2-8℃保(bao)存(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)(la)(la)根(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記親(qin)(qin)和(he)(he)素(su)(su)(su)(su)(su)(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)請(qing)依(yi)據(ju)所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)配(pei)(pei)(pei)(pei)(pei)(pei)置(zhi)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)勿重(zhong)復(fu)(fu)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)(huo)(huo)、辣(la)(la)(la)根(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記親(qin)(qin)和(he)(he)素(su)(su)(su)(su)(su)(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5.建議檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)均設雙孔(kong)(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)，以(yi)保(bao)證檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)結果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)。 植(zhi)物(wu)(wu)(wu)(wu)(wu)硝(xiao)(xiao)酸(suan)(suan)(suan)(suan)還(huan)(huan)原(yuan)(yuan)(yuan)酶(mei)(mei)(mei)(NR)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)方(fang)(fang)(fang)(fang)法手(shou)工(gong)(gong)(gong)洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)方(fang)(fang)(fang)(fang)法：吸去(qu)（不(bu)可(ke)(ke)(ke)觸(chu)及板(ban)(ban)(ban)(ban)壁）或(huo)(huo)(huo)(huo)(huo)(huo)甩掉酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)內(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)；在(zai)(zai)(zai)(zai)(zai)(zai)實(shi)驗(yan)(yan)(yan)(yan)臺上(shang)(shang)(shang)鋪墊(dian)幾層吸水紙(zhi)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)朝下(xia)(xia)(xia)用(yong)(yong)(yong)(yong)(yong)(yong)力拍幾次(ci)(ci)(ci)(ci)(ci)；將(jiang)(jiang)(jiang)(jiang)推(tui)薦(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)(xi)滌(di)(di)緩(huan)沖液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)至(zhi)少(shao)0.3ml注(zhu)(zhu)入(ru)(ru)(ru)孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)，浸泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。根(gen)據(ju)需(xu)要(yao)(yao)，重(zhong)復(fu)(fu)此(ci)(ci)過(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)數(shu)次(ci)(ci)(ci)(ci)(ci)。 自動洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)：如(ru)(ru)果(guo)有(you)(you)自動洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)機(ji)，應(ying)(ying)(ying)在(zai)(zai)(zai)(zai)(zai)(zai)熟(shu)練(lian)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)(hou)再(zai)(zai)(zai)用(yong)(yong)(yong)(yong)(yong)(yong)到正(zheng)式(shi)(shi)實(shi)驗(yan)(yan)(yan)(yan)過(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)中(zhong)(zhong)。 計算(suan)(suan)(suan) 以(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)為(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通(tong)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)(zai)(zai)(zai)半對數(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)，根(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)查出(chu)相應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)；再(zai)(zai)(zai)乘(cheng)以(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)；或(huo)(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)與OD值(zhi)(zhi)(zhi)計算(suan)(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)回(hui)歸方(fang)(fang)(fang)(fang)程(cheng)(cheng)(cheng)式(shi)(shi)，將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)(ru)(ru)方(fang)(fang)(fang)(fang)程(cheng)(cheng)(cheng)式(shi)(shi)，計算(suan)(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)，再(zai)(zai)(zai)乘(cheng)以(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)，即為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際(ji)濃(nong)(nong)(nong)(nong)度(du)(du)(du)。 注(zhu)(zhu)意事(shi)項(xiang)1.當混合(he)蛋白(bai)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)盡(jin)(jin)量(liang)(liang)輕緩(huan)，避(bi)免(mian)(mian)起(qi)泡(pao)(pao)。2.洗(xi)(xi)(xi)滌(di)(di)過(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)非常重(zhong)要(yao)(yao)，不(bu)充分(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)(xi)滌(di)(di)易(yi)造成假陽性(xing)。3.一(yi)(yi)次(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)間**控制(zhi)(zhi)(zhi)在(zai)(zai)(zai)(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)數(shu)量(liang)(liang)多，推(tui)薦(jian)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4.請(qing)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)，做(zuo)復(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)。5.如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)中(zhong)(zhong)待(dai)測(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)質含(han)量(liang)(liang)過(guo)(guo)(guo)(guo)高，請(qing)先(xian)(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)再(zai)(zai)(zai)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)，計算(suan)(suan)(suan)時(shi)(shi)(shi)(shi)(shi)請(qing)乘(cheng)以(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)。6.在(zai)(zai)(zai)(zai)(zai)(zai)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)，請(qing)以(yi)相應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，不(bu)能(neng)混淆(xiao)。7.底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)請(qing)避(bi)光保(bao)存(cun)。 8.不(bu)要(yao)(yao)用(yong)(yong)(yong)(yong)(yong)(yong)其它生(sheng)(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)替(ti)換試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)。 注(zhu)(zhu)意事(shi)項(xiang)：收(shou)(shou)集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)前(qian)(qian)必須清(qing)(qing)楚要(yao)(yao)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成份是(shi)否(fou)足夠穩定(ding)(ding)。對收(shou)(shou)集(ji)后(hou)(hou)(hou)(hou)當天(tian)進(jin)行檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)，儲存(cun)在(zai)(zai)(zai)(zai)(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)有(you)(you)特殊(shu)原(yuan)(yuan)(yuan)因(yin)需(xu)要(yao)(yao)周期收(shou)(shou)集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)， 將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)及時(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)裝(zhuang)后(hou)(hou)(hou)(hou)放(fang)在(zai)(zai)(zai)(zai)(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)(huo)(huo)-70 ℃條件(jian)下(xia)(xia)(xia)保(bao)存(cun)。避(bi)免(mian)(mian)反(fan)復(fu)(fu)凍融。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)2 -8 ℃可(ke)(ke)(ke)保(bao)存(cun)48 小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)(ke)保(bao)存(cun)1 個月。-70度(du)(du)(du)可(ke)(ke)(ke)保(bao)存(cun)6 個月。部(bu)分(fen)(fen)(fen)(fen)激素(su)(su)(su)(su)(su)(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)需(xu)添加(jia)(jia)(jia)(jia)(jia)抑肽(tai)酶(mei)(mei)(mei)。計算(suan)(suan)(suan)：以(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)(zai)(zai)(zai)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)，根(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)查出(chu)相應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)；再(zai)(zai)(zai)乘(cheng)以(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)；或(huo)(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)與OD值(zhi)(zhi)(zhi)計算(suan)(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)回(hui)歸方(fang)(fang)(fang)(fang)程(cheng)(cheng)(cheng)式(shi)(shi)，將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)(ru)(ru)方(fang)(fang)(fang)(fang)程(cheng)(cheng)(cheng)式(shi)(shi)，計算(suan)(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)，再(zai)(zai)(zai)乘(cheng)以(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)，即為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際(ji)濃(nong)(nong)(nong)(nong)度(du)(du)(du)。 植(zhi)物(wu)(wu)(wu)(wu)(wu)硝(xiao)(xiao)酸(suan)(suan)(suan)(suan)還(huan)(huan)原(yuan)(yuan)(yuan)酶(mei)(mei)(mei)(NR)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)性(xing)能(neng)1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)性(xing)回(hui)歸與預(yu)期濃(nong)(nong)(nong)(nong)度(du)(du)(du)相關(guan)(guan)系(xi)數(shu)R值(zhi)(zhi)(zhi)為(wei)(wei)0.92以(yi)上(shang)(shang)(shang)。2.批(pi)內(nei)(nei)與批(pi)見(jian)應(ying)(ying)(ying)分(fen)(fen)(fen)(fen)別(bie)小(xiao)(xiao)于(yu)9%和(he)(he)15% 植(zhi)物(wu)(wu)(wu)(wu)(wu)硝(xiao)(xiao)酸(suan)(suan)(suan)(suan)還(huan)(huan)原(yuan)(yuan)(yuan)酶(mei)(mei)(mei)(NR)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)
實(shi)(shi)驗(yan)原(yuan)理(li) 試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)采用(yong)(yong)(yong)(yong)雙(shuang)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)一步夾心(xin)法(fa)(fa)酶(mei)(mei)(mei)(mei)(mei)聯免(mian)(mian)疫吸附(fu)試(shi)(shi)(shi)驗(yan)（ELISA）。往預先(xian)包被Rubisco活(huo)化(hua)酶(mei)(mei)(mei)(mei)(mei)(RCA)捕獲抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，依(yi)(yi)次加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)，經過(guo)(guo)(guo)(guo)溫育并(bing)徹底(di)洗(xi)(xi)(xi)(xi)滌(di)。用(yong)(yong)(yong)(yong)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯色(se)，TMB在(zai)過(guo)(guo)(guo)(guo)氧化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)下(xia)轉(zhuan)(zhuan)化(hua)成藍(lan)色(se)，并(bing)在(zai)酸(suan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)下(xia)轉(zhuan)(zhuan)化(hua)成＊終(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)。顏色(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Rubisco活(huo)化(hua)酶(mei)(mei)(mei)(mei)(mei)(RCA)呈(cheng)正相(xiang)(xiang)(xiang)(xiang)關。用(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)450nm 波(bo)長下(xia)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定吸光(guang)度(du)(du)(du)(du)(du)（OD 值(zhi)(zhi)(zhi)），計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)。保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)條件及(ji)有(you)(you)(you)效期1.本(ben)(ben)(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)用(yong)(yong)(yong)(yong)于(yu)(yu)體(ti)(ti)(ti)(ti)外定量(liang)(liang)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)血(xue)(xue)清、血(xue)(xue)漿、組織勻(yun)(yun)漿及(ji)相(xiang)(xiang)(xiang)(xiang)關液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)中(zhong)(zhong)Rubisco活(huo)化(hua)酶(mei)(mei)(mei)(mei)(mei)(RCA)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)量(liang)(liang)；2.試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)：2-8℃；3.有(you)(you)(you)效期：6個月(yue)。植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)Rubisco活(huo)化(hua)酶(mei)(mei)(mei)(mei)(mei)(RCA)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采集(ji)(ji)(ji)及(ji)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)1.血(xue)(xue)清：全血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)請(qing)(qing)(qing)(qing)(qing)于(yu)(yu)室溫放置(zhi)2小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)4℃過(guo)(guo)(guo)(guo)一晚后于(yu)(yu)1000 x g離(li)(li)心(xin)20分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，取(qu)上(shang)(shang)(shang)(shang)清即(ji)可(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放于(yu)(yu)-20℃或(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)，但(dan)應(ying)(ying)避(bi)免(mian)(mian)反(fan)復(fu)(fu)(fu)凍融(rong)。 2.血(xue)(xue)漿：可(ke)(ke)用(yong)(yong)(yong)(yong)EDTA或(huo)(huo)肝素(su)(su)(su)(su)作(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)抗(kang)(kang)(kang)凝劑(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)采集(ji)(ji)(ji)后30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)于(yu)(yu)2 - 8° C 1000 x g離(li)(li)心(xin)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，或(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放于(yu)(yu)-20℃或(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)，但(dan)應(ying)(ying)避(bi)免(mian)(mian)反(fan)復(fu)(fu)(fu)凍融(rong)。 3.細胞培養(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)(shang)清或(huo)(huo)其它生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)：1000 x g離(li)(li)心(xin)20分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，取(qu)上(shang)(shang)(shang)(shang)清即(ji)可(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放于(yu)(yu)-20℃或(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)，但(dan)應(ying)(ying)避(bi)免(mian)(mian)反(fan)復(fu)(fu)(fu)凍融(rong)。 (注(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)溶(rong)(rong)血(xue)(xue)會影(ying)響(xiang)**檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)結果(guo)，因此溶(rong)(rong)血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)不(bu)(bu)宜進(jin)行(xing)(xing)此項檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)。) 植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)Rubisco活(huo)化(hua)酶(mei)(mei)(mei)(mei)(mei)(RCA)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he) 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：首先(xian)通過(guo)(guo)(guo)(guo)文(wen)獻(xian)檢(jian)(jian)(jian)(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)式(shi)(shi)了(le)解待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大(da)致含(han)量(liang)(liang)，確定適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)。只(zhi)有(you)(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍(wei)內(nei)(nei)(nei)(nei)，檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結果(guo)才是(shi)準(zhun)(zhun)(zhun)(zhun)確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)程中(zhong)(zhong)，應(ying)(ying)做(zuo)好(hao)詳(xiang)細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)錄。計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)了(le)“N”倍，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)應(ying)(ying)再(zai)乘(cheng)(cheng)以(yi)(yi)(yi)“N”。 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：2瓶，每(mei)(mei)(mei)(mei)(mei)瓶臨用(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)至1ml，蓋(gai)好(hao)后靜置(zhi)10分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)(yi)上(shang)(shang)(shang)(shang)，然后反(fan)復(fu)(fu)(fu)顛倒/搓(cuo)動(dong)以(yi)(yi)(yi)助溶(rong)(rong)解，其濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)300 pg/ml，做(zuo)系列倍比(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后，分(fen)(fen)(fen)(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)直(zhi)接作(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)前(qian)(qian)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 如(ru)(ru)(ru)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取(qu)0.5ml（不(bu)(bu)要(yao)(yao)(yao)少于(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)含(han)0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)中(zhong)(zhong)，混(hun)(hun)(hun)(hun)勻(yun)(yun)即(ji)可(ke)(ke)，其余(yu)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)以(yi)(yi)(yi)此類(lei)推(tui)。 生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：臨用(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)前(qian)(qian)根(gen)(gen)據(ju)預先(xian)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)(mei)次實(shi)(shi)驗(yan)所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)際配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)多(duo)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)10μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(li)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)(hun)(hun)(hun)勻(yun)(yun)，在(zai)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前(qian)(qian)一小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 辣(la)根(gen)(gen)過(guo)(guo)(guo)(guo)氧化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：臨用(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)辣(la)根(gen)(gen)過(guo)(guo)(guo)(guo)氧化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)前(qian)(qian)根(gen)(gen)據(ju)預先(xian)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)(mei)次實(shi)(shi)驗(yan)所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)際配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)多(duo)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)10μl辣(la)根(gen)(gen)過(guo)(guo)(guo)(guo)氧化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl辣(la)根(gen)(gen)過(guo)(guo)(guo)(guo)氧化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(li)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)(hun)(hun)(hun)勻(yun)(yun)，在(zai)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前(qian)(qian)一小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)Rubisco活(huo)化(hua)酶(mei)(mei)(mei)(mei)(mei)(RCA)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)操作(zuo)(zuo)(zuo)步驟(zou) 實(shi)(shi)驗(yan)開始前(qian)(qian)，請(qing)(qing)(qing)(qing)(qing)提前(qian)(qian)配(pei)(pei)(pei)(pei)(pei)置(zhi)好(hao)所有(you)(you)(you)試(shi)(shi)(shi)劑(ji)(ji)(ji)，試(shi)(shi)(shi)劑(ji)(ji)(ji)或(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)，均需(xu)混(hun)(hun)(hun)(hun)勻(yun)(yun)，混(hun)(hun)(hun)(hun)勻(yun)(yun)時(shi)(shi)(shi)(shi)(shi)(shi)盡(jin)量(liang)(liang)避(bi)免(mian)(mian)起泡。每(mei)(mei)(mei)(mei)(mei)次檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)該(gai)(gai)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)。如(ru)(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)過(guo)(guo)(guo)(guo)高時(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)進(jin)行(xing)(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)使(shi)(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)范圍(wei)。 1.加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)別(bie)設空(kong)白孔(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)、待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)。空(kong)白孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)100μl，余(yu)孔(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)意(yi)(yi)不(bu)(bu)要(yao)(yao)(yao)有(you)(you)(you)氣泡，加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)(yu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)底(di)部(bu)，盡(jin)量(liang)(liang)不(bu)(bu)觸及(ji)孔(kong)(kong)(kong)(kong)(kong)壁，輕(qing)輕(qing)晃動(dong)混(hun)(hun)(hun)(hun)勻(yun)(yun)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)(shang)蓋(gai)或(huo)(huo)覆膜(mo)，37℃反(fan)應(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)(wei)保(bao)(bao)(bao)(bao)(bao)(bao)證(zheng)實(shi)(shi)驗(yan)結果(guo)有(you)(you)(you)效性(xing)，每(mei)(mei)(mei)(mei)(mei)次實(shi)(shi)驗(yan)請(qing)(qing)(qing)(qing)(qing)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)。 2.棄(qi)去(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)干(gan)，不(bu)(bu)用(yong)(yong)(yong)(yong)洗(xi)(xi)(xi)(xi)滌(di)。每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye) 100μl（取(qu)1μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(li)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)(hun)(hun)(hun)勻(yun)(yun)，在(zai)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前(qian)(qian)一小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 3.溫育60分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后，棄(qi)去(qu)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)干(gan)，洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)3次，每(mei)(mei)(mei)(mei)(mei)次浸(jin)(jin)泡1-2分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)干(gan)。4.每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)辣(la)根(gen)(gen)過(guo)(guo)(guo)(guo)氧化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)工(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)（同生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。5.溫育60分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后，棄(qi)去(qu)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)干(gan)，洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)5次，每(mei)(mei)(mei)(mei)(mei)次浸(jin)(jin)泡1-2分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)干(gan)。6.依(yi)(yi)序(xu)(xu)每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)光(guang)顯色(se)（30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)，此時(shi)(shi)(shi)(shi)(shi)(shi)肉眼可(ke)(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)有(you)(you)(you)明顯的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)(du)(du)藍(lan)色(se)，后3-4孔(kong)(kong)(kong)(kong)(kong)梯度(du)(du)(du)(du)(du)不(bu)(bu)明顯，即(ji)可(ke)(ke)終(zhong)(zhong)止(zhi)(zhi)）。7.依(yi)(yi)序(xu)(xu)每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)止(zhi)(zhi)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)止(zhi)(zhi)反(fan)應(ying)(ying)（此時(shi)(shi)(shi)(shi)(shi)(shi)藍(lan)色(se)立轉(zhuan)(zhuan)黃色(se)）。終(zhong)(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)順序(xu)(xu)應(ying)(ying)盡(jin)量(liang)(liang)與底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)順序(xu)(xu)相(xiang)(xiang)(xiang)(xiang)同。為(wei)(wei)(wei)(wei)了(le)保(bao)(bao)(bao)(bao)(bao)(bao)證(zheng)實(shi)(shi)驗(yan)結果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)確性(xing)，底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)應(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)間到后應(ying)(ying)盡(jin)快加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)終(zhong)(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)聯儀在(zai)450nm波(bo)長依(yi)(yi)序(xu)(xu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)各(ge)孔(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)密度(du)(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)）。 在(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)后15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)(yi)內(nei)(nei)(nei)(nei)進(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)。植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)Rubisco活(huo)化(hua)酶(mei)(mei)(mei)(mei)(mei)(RCA)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)注(zhu)意(yi)(yi)事(shi)(shi)項1.用(yong)(yong)(yong)(yong)戶在(zai)初次使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)時(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)將(jiang)(jiang)(jiang)(jiang)各(ge)種試(shi)(shi)(shi)劑(ji)(ji)(ji)管(guan)離(li)(li)心(xin)數(shu)分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，以(yi)(yi)(yi)便試(shi)(shi)(shi)劑(ji)(ji)(ji)集(ji)(ji)(ji)中(zhong)(zhong)到管(guan)底(di)。2.每(mei)(mei)(mei)(mei)(mei)次實(shi)(shi)驗(yan)留一孔(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)空(kong)白調(diao)零孔(kong)(kong)(kong)(kong)(kong)，該(gai)(gai)孔(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)任何試(shi)(shi)(shi)劑(ji)(ji)(ji)，只(zhi)是(shi)**加(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)及(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)時(shi)(shi)(shi)(shi)(shi)(shi)先(xian)用(yong)(yong)(yong)(yong)此孔(kong)(kong)(kong)(kong)(kong)調(diao)OD值(zhi)(zhi)(zhi)至零。 3.為(wei)(wei)(wei)(wei)防止(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)驗(yan)時(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)(jiang)(jiang)反(fan)應(ying)(ying)板(ban)(ban)(ban)放于(yu)(yu)鋪有(you)(you)(you)濕布(bu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉(bi)盒(he)內(nei)(nei)(nei)(nei)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)(shang)蓋(gai)或(huo)(huo)覆膜(mo)。 4.未使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)或(huo)(huo)者試(shi)(shi)(shi)劑(ji)(ji)(ji)，請(qing)(qing)(qing)(qing)(qing)于(yu)(yu)2-8℃保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)、辣(la)根(gen)(gen)過(guo)(guo)(guo)(guo)氧化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)工(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)請(qing)(qing)(qing)(qing)(qing)依(yi)(yi)據(ju)所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)配(pei)(pei)(pei)(pei)(pei)置(zhi)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)。請(qing)(qing)(qing)(qing)(qing)勿重復(fu)(fu)(fu)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)或(huo)(huo)、辣(la)根(gen)(gen)過(guo)(guo)(guo)(guo)氧化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)工(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)。 5.建(jian)議檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)均設雙(shuang)孔(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定，以(yi)(yi)(yi)保(bao)(bao)(bao)(bao)(bao)(bao)證(zheng)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)結果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)確性(xing)。 植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)Rubisco活(huo)化(hua)酶(mei)(mei)(mei)(mei)(mei)(RCA)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)方(fang)法(fa)(fa)手工(gong)(gong)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)方(fang)法(fa)(fa)：吸去(qu)（不(bu)(bu)可(ke)(ke)觸及(ji)板(ban)(ban)(ban)壁）或(huo)(huo)甩(shuai)掉酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)內(nei)(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)；在(zai)實(shi)(shi)驗(yan)臺上(shang)(shang)(shang)(shang)鋪墊(dian)幾(ji)層吸水紙(zhi)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)朝下(xia)用(yong)(yong)(yong)(yong)力(li)拍幾(ji)次；將(jiang)(jiang)(jiang)(jiang)推(tui)薦(jian)(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)(xi)(xi)滌(di)緩(huan)沖液(ye)(ye)(ye)(ye)(ye)至少0.3ml注(zhu)入(ru)(ru)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)，浸(jin)(jin)泡1-2分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。根(gen)(gen)據(ju)需(xu)要(yao)(yao)(yao)，重復(fu)(fu)(fu)此過(guo)(guo)(guo)(guo)程數(shu)次。 自動(dong)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)：如(ru)(ru)(ru)果(guo)有(you)(you)(you)自動(dong)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)機(ji)，應(ying)(ying)在(zai)熟練使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)后再(zai)用(yong)(yong)(yong)(yong)到正式(shi)(shi)實(shi)(shi)驗(yan)過(guo)(guo)(guo)(guo)程中(zhong)(zhong)。 計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan) 以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對(dui)數(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)半對(dui)數(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)(shang)(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)，根(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)查出(chu)相(xiang)(xiang)(xiang)(xiang)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)；再(zai)乘(cheng)(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)；或(huo)(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)與OD值(zhi)(zhi)(zhi)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)(xian)(xian)回歸方(fang)程式(shi)(shi)，將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)(ru)方(fang)程式(shi)(shi)，計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)，再(zai)乘(cheng)(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)，即(ji)為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)。 注(zhu)意(yi)(yi)事(shi)(shi)項1.當混(hun)(hun)(hun)(hun)合蛋白溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)盡(jin)量(liang)(liang)輕(qing)緩(huan)，避(bi)免(mian)(mian)起泡。2.洗(xi)(xi)(xi)(xi)滌(di)過(guo)(guo)(guo)(guo)程非常重要(yao)(yao)(yao)，不(bu)(bu)充分(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)(xi)(xi)滌(di)易造成假陽(yang)性(xing)。3.一次加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)間**控制(zhi)(zhi)(zhi)在(zai)5分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)，如(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)數(shu)量(liang)(liang)多(duo)，推(tui)薦(jian)(jian)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)。4.請(qing)(qing)(qing)(qing)(qing)每(mei)(mei)(mei)(mei)(mei)次測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)，做(zuo)復(fu)(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)。5.如(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)中(zhong)(zhong)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含(han)量(liang)(liang)過(guo)(guo)(guo)(guo)高，請(qing)(qing)(qing)(qing)(qing)先(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后再(zai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定，計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)時(shi)(shi)(shi)(shi)(shi)(shi)請(qing)(qing)(qing)(qing)(qing)乘(cheng)(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)。6.在(zai)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)工(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)(qing)(qing)(qing)(qing)以(yi)(yi)(yi)相(xiang)(xiang)(xiang)(xiang)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，不(bu)(bu)能(neng)混(hun)(hun)(hun)(hun)淆(xiao)。7.底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)(qing)(qing)(qing)避(bi)光(guang)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)。 8.不(bu)(bu)要(yao)(yao)(yao)用(yong)(yong)(yong)(yong)其它生(sheng)(sheng)(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)替換(huan)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)。 注(zhu)意(yi)(yi)事(shi)(shi)項：收集(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)前(qian)(qian)必(bi)須清楚要(yao)(yao)(yao)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成份是(shi)否足夠(gou)穩(wen)定。對(dui)收集(ji)(ji)(ji)后當天進(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)，儲存(cun)(cun)在(zai)4 ℃備用(yong)(yong)(yong)(yong)，如(ru)(ru)(ru)有(you)(you)(you)特殊原(yuan)因需(xu)要(yao)(yao)(yao)周期收集(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)， 將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)及(ji)時(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)裝(zhuang)后放在(zai)-20 ℃或(huo)(huo)-70 ℃條件下(xia)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)。避(bi)免(mian)(mian)反(fan)復(fu)(fu)(fu)凍融(rong)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)2 -8 ℃可(ke)(ke)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)48 小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)1 個月(yue)。-70度(du)(du)(du)(du)(du)可(ke)(ke)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)6 個月(yue)。部(bu)分(fen)(fen)(fen)(fen)(fen)激素(su)(su)(su)(su)類(lei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)需(xu)添(tian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)抑肽酶(mei)(mei)(mei)(mei)(mei)。計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)：以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)(shang)(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)，根(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)查出(chu)相(xiang)(xiang)(xiang)(xiang)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)；再(zai)乘(cheng)(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)；或(huo)(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)與OD值(zhi)(zhi)(zhi)計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)(xian)(xian)回歸方(fang)程式(shi)(shi)，將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)(ru)方(fang)程式(shi)(shi)，計(ji)(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)，再(zai)乘(cheng)(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)，即(ji)為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)。 植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)Rubisco活(huo)化(hua)酶(mei)(mei)(mei)(mei)(mei)(RCA)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)性(xing)能(neng)1.樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)(xian)性(xing)回歸與預期濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)相(xiang)(xiang)(xiang)(xiang)關系數(shu)R值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)0.92以(yi)(yi)(yi)上(shang)(shang)(shang)(shang)。2.批(pi)內(nei)(nei)(nei)(nei)與批(pi)見應(ying)(ying)分(fen)(fen)(fen)(fen)(fen)別(bie)小(xiao)(xiao)于(yu)(yu)9%和(he)(he)(he)15% 植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)Rubisco活(huo)化(hua)酶(mei)(mei)(mei)(mei)(mei)(RCA)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)
322