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植(zhi)物(wu)(wu)組織(zhi)蔗(zhe)(zhe)(zhe)糖(tang)(tang)(tang)(tang)(tang)(tang)含(han)量檢測(ce)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒說明(ming)書 微(wei)(wei)量法  注意：正式(shi)測(ce)定(ding)(ding)(ding)(ding)(ding)前務必取(qu)(qu)(qu) 2-3 個預期差異較大的樣(yang)(yang)本(ben)做預測(ce)定(ding)(ding)(ding)(ding)(ding)。 貨號：YX-W-B502 規格(ge)：100T/96S 產品內容： 提(ti)取(qu)(qu)(qu)液(ye)(ye)：液(ye)(ye)體(ti)(ti) 100mL×1 瓶，4℃保(bao)(bao)存；  試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)一(yi)：粉(fen)劑(ji)(ji)(ji)(ji) 10mg×1 支，4℃保(bao)(bao)存，臨用(yong)(yong)前加入 1mL 蒸(zheng)(zheng)餾(liu)水(shui)溶解，用(yong)(yong)水(shui)稀釋 10 倍，備用(yong)(yong)，即 1mg/mL; 試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)二(er)(er)：液(ye)(ye)體(ti)(ti) 2mL×1 瓶，4℃保(bao)(bao)存； 試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)三：液(ye)(ye)體(ti)(ti) 20mL×1 瓶，4℃保(bao)(bao)存； 試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)四：液(ye)(ye)體(ti)(ti) 5mL×1 瓶，4℃保(bao)(bao)存； 試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)五：粉(fen)劑(ji)(ji)(ji)(ji) 0.5g×1 瓶，常(chang)(chang)溫保(bao)(bao)存。 植(zhi)物(wu)(wu)組織(zhi)蔗(zhe)(zhe)(zhe)糖(tang)(tang)(tang)(tang)(tang)(tang)含(han)量檢測(ce)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒說明(ming)書 產品介(jie)紹： 蔗(zhe)(zhe)(zhe)糖(tang)(tang)(tang)(tang)(tang)(tang)是(shi)植(zhi)物(wu)(wu)光合(he)作(zuo)用(yong)(yong)的主(zhu)要產物(wu)(wu)，也是(shi)糖(tang)(tang)(tang)(tang)(tang)(tang)分(fen)運輸和(he)(he)(he)儲藏的主(zhu)要形式(shi)。因(yin)此，測(ce)定(ding)(ding)(ding)(ding)(ding)蔗(zhe)(zhe)(zhe)糖(tang)(tang)(tang)(tang)(tang)(tang)含(han)量對 于植(zhi)物(wu)(wu)糖(tang)(tang)(tang)(tang)(tang)(tang)代謝具有重(zhong)要意義。此外，蔗(zhe)(zhe)(zhe)糖(tang)(tang)(tang)(tang)(tang)(tang)含(han)量是(shi)飲料﹑蜂蜜(mi)、果脯﹑糖(tang)(tang)(tang)(tang)(tang)(tang)果和(he)(he)(he)乳(ru)制品等產品質量控(kong) 制的重(zhong)要指標(biao)(biao)之一(yi)。 先用(yong)(yong)堿與樣(yang)(yang)品共熱(re)，破(po)壞其(qi)中的還原糖(tang)(tang)(tang)(tang)(tang)(tang)。酸性條件(jian)下(xia)蔗(zhe)(zhe)(zhe)糖(tang)(tang)(tang)(tang)(tang)(tang)水(shui)解生成葡萄(tao)糖(tang)(tang)(tang)(tang)(tang)(tang)和(he)(he)(he)果糖(tang)(tang)(tang)(tang)(tang)(tang)，果糖(tang)(tang)(tang)(tang)(tang)(tang)進一(yi) 步(bu)與間苯(ben)二(er)(er)酚反(fan)應(ying)，生成有色物(wu)(wu)質，在 480nm 下(xia)有特征吸收峰。 所需的儀(yi)器和(he)(he)(he)用(yong)(yong)品： 可見(jian)分(fen)光光度(du)計(ji)(ji)/酶標(biao)(biao)儀(yi)、水(shui)浴鍋(guo)、可調式(shi)移液(ye)(ye)器、微(wei)(wei)量玻璃比(bi)色皿/96 孔板(ban)和(he)(he)(he)蒸(zheng)(zheng)餾(liu)水(shui)。 操作(zuo)步(bu)驟 一(yi)、樣(yang)(yang)品制備： 稱取(qu)(qu)(qu)0.1g 樣(yang)(yang)本(ben)，常(chang)(chang)溫研碎，加入0.5mL 提(ti)取(qu)(qu)(qu)液(ye)(ye)，適當(dang)研磨后快速轉移到離(li)心(xin)(xin)管(guan)(guan)中，置于80℃水(shui)浴鍋(guo)中 10min，振蕩3~5 次，冷(leng)卻(que)后，4000g，25℃離(li)心(xin)(xin)10min，取(qu)(qu)(qu)上清，加入2mg試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)五，80℃脫色30min， 再加入0.5mL 提(ti)取(qu)(qu)(qu)液(ye)(ye)，4000g，25℃離(li)心(xin)(xin)10min，取(qu)(qu)(qu)上清液(ye)(ye)測(ce)定(ding)(ding)(ding)(ding)(ding)。 二(er)(er)、測(ce)定(ding)(ding)(ding)(ding)(ding)步(bu)驟： 1、分(fen)光光度(du)計(ji)(ji)或酶標(biao)(biao)儀(yi)預熱(re) 30min 以上，調節波長至(zhi) 480nm，蒸(zheng)(zheng)餾(liu)水(shui)調零。 2、樣(yang)(yang)本(ben)測(ce)定(ding)(ding)(ding)(ding)(ding)，（在 1.5mL EP 管(guan)(guan)中依(yi)次加入下(xia)列(lie)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)）： 試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)（μL）空白(bai)管(guan)(guan)標(biao)(biao)準管(guan)(guan)測(ce)定(ding)(ding)(ding)(ding)(ding)管(guan)(guan)樣(yang)(yang)本(ben)25 試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)一(yi)25 蒸(zheng)(zheng)餾(liu)水(shui)25 試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)二(er)(er)151515混勻，100℃煮沸(fei) 5min 左(zuo)右（蓋緊(jin)，防止(zhi)水(shui)分(fen)散失）試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)三175175175試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)四505050混勻，沸(fei)水(shui)浴反(fan)應(ying) 10min 左(zuo)右，冷(leng)卻(que)后取(qu)(qu)(qu) 200μL 至(zhi)微(wei)(wei)量玻璃比(bi)色皿或 96 孔板(ban)中測(ce)定(ding)(ding)(ding)(ding)(ding) 480nm 處(chu)光吸 收值，空白(bai)管(guan)(guan)、標(biao)(biao)準管(guan)(guan)和(he)(he)(he)測(ce)定(ding)(ding)(ding)(ding)(ding)管(guan)(guan)分(fen)別記(ji)為(wei) A1、A2 和(he)(he)(he) A3 三、蔗(zhe)(zhe)(zhe)糖(tang)(tang)(tang)(tang)(tang)(tang)含(han)量計(ji)(ji)算： 1、按照(zhao)蛋白(bai)質含(han)量計(ji)(ji)算 蔗(zhe)(zhe)(zhe)糖(tang)(tang)(tang)(tang)(tang)(tang)含(han)量（mg/mg prot）= (C 標(biao)(biao)準管(guan)(guan)×V1)×(A3-A1)÷(A2-A1) ÷（V1×Cpr）= (A3-A1)÷(A2-A1) ÷Cpr 此法需要自(zi)行測(ce)定(ding)(ding)(ding)(ding)(ding)蛋白(bai)濃度(du)。 2、按照(zhao)樣(yang)(yang)品質量計(ji)(ji)算 蔗(zhe)(zhe)(zhe)糖(tang)(tang)(tang)(tang)(tang)(tang)含(han)量(mg/g 鮮重(zhong)）=(C 標(biao)(biao)準×V1)×(A3-A1)÷(A2-A1)÷(W×V1÷V2)=(A3-A1)÷(A2-A1)÷W C 標(biao)(biao)準管(guan)(guan)：標(biao)(biao)準管(guan)(guan)濃度(du)，1mg/mL；V1：加入樣(yang)(yang)本(ben)體(ti)(ti)積，0.025mL；V2：加入提(ti)取(qu)(qu)(qu)液(ye)(ye)體(ti)(ti)積，1mL； Cpr：樣(yang)(yang)本(ben)蛋白(bai)質濃度(du)，mg/mL；W：樣(yang)(yang)本(ben)鮮重(zhong)，g。 本(ben)產品僅供科學研究使用(yong)(yong)！請勿用(yong)(yong)于臨床、診斷、食品、化妝品檢測(ce)等用(yong)(yong)途！

實(shi)驗(yan)(yan)原(yuan)(yuan)理   試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)采用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙(shuang)抗(kang)(kang)(kang)(kang)體(ti)(ti)一(yi)步夾心(xin)法酶(mei)(mei)(mei)(mei)(mei)聯免(mian)疫吸(xi)附試(shi)(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)（ELISA）。往預(yu)(yu)(yu)先(xian)(xian)包被植(zhi)(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)多(duo)糖(tang)(tang)(Pol)捕獲(huo)抗(kang)(kang)(kang)(kang)體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，依(yi)次(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)抗(kang)(kang)(kang)(kang)體(ti)(ti)，經過(guo)(guo)溫(wen)育(yu)并徹(che)底(di)(di)(di)洗滌。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯色(se)，TMB在(zai)(zai)(zai)(zai)(zai)過(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催(cui)化(hua)下(xia)轉化(hua)成(cheng)藍(lan)色(se)，并在(zai)(zai)(zai)(zai)(zai)酸(suan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)下(xia)轉化(hua)成(cheng)＊終(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)色(se)。顏色(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)植(zhi)(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)多(duo)糖(tang)(tang)(Pol)呈(cheng)正相(xiang)(xiang)(xiang)關(guan)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)(zai)(zai)(zai)450nm 波(bo)(bo)長下(xia)測(ce)(ce)(ce)定吸(xi)光(guang)度(du)(du)(du)(du)(du)(du)（OD 值(zhi)(zhi)(zhi)(zhi)(zhi)），計算(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)。保(bao)(bao)存(cun)(cun)條件及(ji)有(you)效期1.本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)體(ti)(ti)外(wai)定量(liang)(liang)(liang)(liang)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)血清(qing)、血漿(jiang)(jiang)、組織勻(yun)(yun)(yun)(yun)漿(jiang)(jiang)及(ji)相(xiang)(xiang)(xiang)關(guan)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)植(zhi)(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)多(duo)糖(tang)(tang)(Pol)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)(liang)(liang)；2.試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)保(bao)(bao)存(cun)(cun)：2-8℃；3.有(you)效期：6個月。植(zhi)(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)多(duo)糖(tang)(tang)(Pol)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采集(ji)(ji)(ji)(ji)及(ji)保(bao)(bao)存(cun)(cun)1.血清(qing)：全血標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)請(qing)于(yu)(yu)室溫(wen)放(fang)(fang)置2小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)4℃過(guo)(guo)一(yi)晚后(hou)(hou)于(yu)(yu)1000 x g離(li)心(xin)20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，取上(shang)(shang)清(qing)即可(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)，或(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)(fang)于(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)(bi)免(mian)反(fan)復(fu)凍(dong)融。 2.血漿(jiang)(jiang)：可(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)肝素(su)(su)(su)(su)(su)作(zuo)為(wei)(wei)(wei)(wei)抗(kang)(kang)(kang)(kang)凝劑(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)采集(ji)(ji)(ji)(ji)后(hou)(hou)30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內于(yu)(yu)2 - 8° C 1000 x g離(li)心(xin)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)(fang)于(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)(bi)免(mian)反(fan)復(fu)凍(dong)融。 3.細(xi)胞培養(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)清(qing)或(huo)(huo)(huo)其它生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)：1000 x g離(li)心(xin)20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，取上(shang)(shang)清(qing)即可(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)，或(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)(fang)于(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)(bi)免(mian)反(fan)復(fu)凍(dong)融。 (注：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)溶血會影(ying)響(xiang)**檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)結(jie)果(guo)(guo)，因此(ci)(ci)溶血標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)不(bu)(bu)(bu)(bu)宜進(jin)(jin)(jin)行(xing)此(ci)(ci)項檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)。) 植(zhi)(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)多(duo)糖(tang)(tang)(Pol)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)   標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)原(yuan)(yuan)則：首先(xian)(xian)通過(guo)(guo)文獻檢(jian)(jian)(jian)(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)式(shi)(shi)了(le)解(jie)待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大(da)致含量(liang)(liang)(liang)(liang)，確(que)定適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)倍(bei)數(shu)(shu)。只(zhi)有(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍(wei)內，檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果(guo)(guo)才(cai)是準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)做(zuo)(zuo)(zuo)(zuo)好(hao)詳(xiang)細(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)錄(lu)。計算(suan)(suan)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)了(le)“N”倍(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)再(zai)(zai)乘以(yi)(yi)(yi)(yi)(yi)“N”。    標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)原(yuan)(yuan)則：2瓶(ping)，每(mei)(mei)(mei)(mei)(mei)瓶(ping)臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)至1ml，蓋好(hao)后(hou)(hou)靜置10分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)上(shang)(shang)，然后(hou)(hou)反(fan)復(fu)顛倒/搓動(dong)以(yi)(yi)(yi)(yi)(yi)助(zhu)溶解(jie)，其濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)300 pg/ml，做(zuo)(zuo)(zuo)(zuo)系列倍(bei)比稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)后(hou)(hou)，分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)直(zhi)接作(zuo)為(wei)(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)。 如(ru)(ru)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取0.5ml（不(bu)(bu)(bu)(bu)要少(shao)于(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)含0.5ml樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)中(zhong)(zhong)(zhong)(zhong)，混(hun)(hun)勻(yun)(yun)(yun)(yun)即可(ke)(ke)，其余濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)以(yi)(yi)(yi)(yi)(yi)此(ci)(ci)類推(tui)。    生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)原(yuan)(yuan)則：臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)(yi)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)前(qian)(qian)根(gen)(gen)(gen)(gen)據(ju)預(yu)(yu)(yu)先(xian)(xian)計算(suan)(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)驗(yan)(yan)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際(ji)(ji)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)多(duo)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)勻(yun)(yun)(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)。    辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)親(qin)和(he)素(su)(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)原(yuan)(yuan)則：臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)(yi)辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)親(qin)和(he)素(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)前(qian)(qian)根(gen)(gen)(gen)(gen)據(ju)預(yu)(yu)(yu)先(xian)(xian)計算(suan)(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)驗(yan)(yan)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際(ji)(ji)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)多(duo)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)親(qin)和(he)素(su)(su)(su)(su)(su)加(jia)(jia)(jia)(jia)(jia)(jia)990μl辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)親(qin)和(he)素(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)勻(yun)(yun)(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)。 植(zhi)(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)多(duo)糖(tang)(tang)(Pol)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)操作(zuo)步驟   實(shi)驗(yan)(yan)開始(shi)前(qian)(qian)，請(qing)提前(qian)(qian)配(pei)(pei)(pei)(pei)置好(hao)所有(you)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)或(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，均需混(hun)(hun)勻(yun)(yun)(yun)(yun)，混(hun)(hun)勻(yun)(yun)(yun)(yun)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盡(jin)量(liang)(liang)(liang)(liang)避(bi)(bi)(bi)免(mian)起泡(pao)。每(mei)(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)(ying)(ying)(ying)該(gai)(gai)做(zuo)(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)。如(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)過(guo)(guo)高(gao)(gao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)進(jin)(jin)(jin)行(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)(yi)使(shi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)范圍(wei)。 1.加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)設空白(bai)孔(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)、待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)。空白(bai)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注意(yi)不(bu)(bu)(bu)(bu)要有(you)氣泡(pao)，加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)于(yu)(yu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)孔(kong)(kong)(kong)(kong)(kong)底(di)(di)(di)部，盡(jin)量(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)觸及(ji)孔(kong)(kong)(kong)(kong)(kong)壁，輕(qing)(qing)(qing)輕(qing)(qing)(qing)晃(huang)動(dong)混(hun)(hun)勻(yun)(yun)(yun)(yun)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)加(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)蓋或(huo)(huo)(huo)覆(fu)膜，37℃反(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)(wei)保(bao)(bao)證(zheng)實(shi)驗(yan)(yan)結(jie)果(guo)(guo)有(you)效性(xing)(xing)(xing)，每(mei)(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)驗(yan)(yan)請(qing)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)新(xin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 2.棄(qi)(qi)去(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，甩干，不(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗滌。每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)工(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)勻(yun)(yun)(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。 3.溫(wen)育(yu)60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)，棄(qi)(qi)去(qu)(qu)孔(kong)(kong)(kong)(kong)(kong)內液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，甩干，洗板(ban)3次(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩干。4.每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)親(qin)和(he)素(su)(su)(su)(su)(su)工(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同(tong)(tong)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)工(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。5.溫(wen)育(yu)60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)，棄(qi)(qi)去(qu)(qu)孔(kong)(kong)(kong)(kong)(kong)內液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，甩干，洗板(ban)5次(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩干。6.依(yi)序每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)(bi)(bi)光(guang)顯色(se)（30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內，此(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)肉眼(yan)可(ke)(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)有(you)明顯的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯(ti)度(du)(du)(du)(du)(du)(du)藍(lan)色(se)，后(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)梯(ti)度(du)(du)(du)(du)(du)(du)不(bu)(bu)(bu)(bu)明顯，即可(ke)(ke)終(zhong)(zhong)止(zhi)(zhi)(zhi)）。7.依(yi)序每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)止(zhi)(zhi)(zhi)溶液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)止(zhi)(zhi)(zhi)反(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)（此(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)藍(lan)色(se)立轉黃(huang)色(se)）。終(zhong)(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)順序應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)量(liang)(liang)(liang)(liang)與(yu)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)順序相(xiang)(xiang)(xiang)同(tong)(tong)。為(wei)(wei)(wei)(wei)了(le)保(bao)(bao)證(zheng)實(shi)驗(yan)(yan)結(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)(xing)，底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)間(jian)到(dao)后(hou)(hou)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)快(kuai)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)終(zhong)(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)聯儀在(zai)(zai)(zai)(zai)(zai)450nm波(bo)(bo)長依(yi)序測(ce)(ce)(ce)量(liang)(liang)(liang)(liang)各孔(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)密度(du)(du)(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)(zhi)）。 在(zai)(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)內進(jin)(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)。植(zhi)(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)多(duo)糖(tang)(tang)(Pol)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)注意(yi)事(shi)項1.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)(zai)(zai)(zai)初次(ci)(ci)(ci)(ci)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)將(jiang)(jiang)(jiang)(jiang)各種試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)管(guan)離(li)心(xin)數(shu)(shu)分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)集(ji)(ji)(ji)(ji)中(zhong)(zhong)(zhong)(zhong)到(dao)管(guan)底(di)(di)(di)。2.每(mei)(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)驗(yan)(yan)留一(yi)孔(kong)(kong)(kong)(kong)(kong)作(zuo)為(wei)(wei)(wei)(wei)空白(bai)調(diao)(diao)零孔(kong)(kong)(kong)(kong)(kong)，該(gai)(gai)孔(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)任何試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，只(zhi)是**加(jia)(jia)(jia)(jia)(jia)(jia)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)及(ji)2N H2SO4。測(ce)(ce)(ce)量(liang)(liang)(liang)(liang)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)先(xian)(xian)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)此(ci)(ci)孔(kong)(kong)(kong)(kong)(kong)調(diao)(diao)OD值(zhi)(zhi)(zhi)(zhi)(zhi)至零。 3.為(wei)(wei)(wei)(wei)防止(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)(jiang)(jiang)反(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)板(ban)放(fang)(fang)于(yu)(yu)鋪有(you)濕(shi)布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉(bi)盒(he)內，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)加(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)蓋或(huo)(huo)(huo)覆(fu)膜。 4.未(wei)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)或(huo)(huo)(huo)者試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，請(qing)于(yu)(yu)2-8℃保(bao)(bao)存(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)工(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)親(qin)和(he)素(su)(su)(su)(su)(su)工(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)請(qing)依(yi)據(ju)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)置使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)勿重復(fu)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)過(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)工(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)、辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)(ji)親(qin)和(he)素(su)(su)(su)(su)(su)工(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5.建議檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)均設雙(shuang)孔(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)定，以(yi)(yi)(yi)(yi)(yi)保(bao)(bao)證(zheng)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)結(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)(xing)。 植(zhi)(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)多(duo)糖(tang)(tang)(Pol)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)洗板(ban)方(fang)(fang)法手工(gong)洗板(ban)方(fang)(fang)法：吸(xi)去(qu)(qu)（不(bu)(bu)(bu)(bu)可(ke)(ke)觸及(ji)板(ban)壁）或(huo)(huo)(huo)甩掉酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)內的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)；在(zai)(zai)(zai)(zai)(zai)實(shi)驗(yan)(yan)臺(tai)上(shang)(shang)鋪墊(dian)幾(ji)層吸(xi)水紙，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)朝下(xia)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)力拍幾(ji)次(ci)(ci)(ci)(ci)；將(jiang)(jiang)(jiang)(jiang)推(tui)薦(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗滌緩沖液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)至少(shao)0.3ml注入(ru)(ru)孔(kong)(kong)(kong)(kong)(kong)內，浸泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。根(gen)(gen)(gen)(gen)據(ju)需要，重復(fu)此(ci)(ci)過(guo)(guo)程數(shu)(shu)次(ci)(ci)(ci)(ci)。 自動(dong)洗板(ban)：如(ru)(ru)果(guo)(guo)有(you)自動(dong)洗板(ban)機，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)在(zai)(zai)(zai)(zai)(zai)熟(shu)練使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)再(zai)(zai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)到(dao)正式(shi)(shi)實(shi)驗(yan)(yan)過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)。 計算(suan)(suan)   以(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱(zong)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)(zai)(zai)半對數(shu)(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)繪(hui)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)，根(gen)(gen)(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)查(cha)出(chu)(chu)(chu)相(xiang)(xiang)(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)；再(zai)(zai)乘以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)倍(bei)數(shu)(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)(zhi)(zhi)計算(suan)(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)回(hui)歸(gui)方(fang)(fang)程式(shi)(shi)，將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)代入(ru)(ru)方(fang)(fang)程式(shi)(shi)，計算(suan)(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)，再(zai)(zai)乘以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)倍(bei)數(shu)(shu)，即為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際(ji)(ji)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)。 注意(yi)事(shi)項1.當混(hun)(hun)合蛋白(bai)溶液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)量(liang)(liang)(liang)(liang)輕(qing)(qing)(qing)緩，避(bi)(bi)(bi)免(mian)起泡(pao)。2.洗滌過(guo)(guo)程非常重要，不(bu)(bu)(bu)(bu)充(chong)分(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗滌易造成(cheng)假陽性(xing)(xing)(xing)。3.一(yi)次(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)間(jian)**控制(zhi)(zhi)(zhi)(zhi)在(zai)(zai)(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)(shu)量(liang)(liang)(liang)(liang)多(duo)，推(tui)薦(jian)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)。4.請(qing)每(mei)(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)定的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)(tong)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)做(zuo)(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)，做(zuo)(zuo)(zuo)(zuo)復(fu)孔(kong)(kong)(kong)(kong)(kong)。5.如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)待測(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)(liang)(liang)(liang)過(guo)(guo)高(gao)(gao)，請(qing)先(xian)(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)后(hou)(hou)再(zai)(zai)測(ce)(ce)(ce)定，計算(suan)(suan)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)請(qing)乘以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)倍(bei)數(shu)(shu)。6.在(zai)(zai)(zai)(zai)(zai)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)溶液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)工(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)以(yi)(yi)(yi)(yi)(yi)相(xiang)(xiang)(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)，不(bu)(bu)(bu)(bu)能(neng)(neng)混(hun)(hun)淆(xiao)。7.底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)避(bi)(bi)(bi)光(guang)保(bao)(bao)存(cun)(cun)。 8.不(bu)(bu)(bu)(bu)要用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)其它生(sheng)(sheng)(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)替換(huan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)。 注意(yi)事(shi)項：收集(ji)(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)前(qian)(qian)必須清(qing)楚要檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)份是否足夠穩定。對收集(ji)(ji)(ji)(ji)后(hou)(hou)當天進(jin)(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)，儲存(cun)(cun)在(zai)(zai)(zai)(zai)(zai)4 ℃備(bei)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)有(you)特(te)殊(shu)原(yuan)(yuan)因需要周期收集(ji)(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)， 將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)及(ji)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)裝后(hou)(hou)放(fang)(fang)在(zai)(zai)(zai)(zai)(zai)-20 ℃或(huo)(huo)(huo)-70 ℃條件下(xia)保(bao)(bao)存(cun)(cun)。避(bi)(bi)(bi)免(mian)反(fan)復(fu)凍(dong)融。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)保(bao)(bao)存(cun)(cun)48 小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)保(bao)(bao)存(cun)(cun)1 個月。-70度(du)(du)(du)(du)(du)(du)可(ke)(ke)保(bao)(bao)存(cun)(cun)6 個月。部分(fen)(fen)(fen)(fen)激素(su)(su)(su)(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)需添(tian)加(jia)(jia)(jia)(jia)(jia)(jia)抑(yi)肽酶(mei)(mei)(mei)(mei)(mei)。計算(suan)(suan)：以(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱(zong)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)繪(hui)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)，根(gen)(gen)(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)查(cha)出(chu)(chu)(chu)相(xiang)(xiang)(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)；再(zai)(zai)乘以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)倍(bei)數(shu)(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)(zhi)(zhi)計算(suan)(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)回(hui)歸(gui)方(fang)(fang)程式(shi)(shi)，將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)代入(ru)(ru)方(fang)(fang)程式(shi)(shi)，計算(suan)(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)，再(zai)(zai)乘以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)倍(bei)數(shu)(shu)，即為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際(ji)(ji)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)。 植(zhi)(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)多(duo)糖(tang)(tang)(Pol)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)性(xing)(xing)(xing)能(neng)(neng)1.樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)性(xing)(xing)(xing)回(hui)歸(gui)與(yu)預(yu)(yu)(yu)期濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)相(xiang)(xiang)(xiang)關(guan)系數(shu)(shu)R值(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)0.92以(yi)(yi)(yi)(yi)(yi)上(shang)(shang)。2.批內與(yu)批見應(ying)(ying)(ying)(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)小(xiao)于(yu)(yu)9%和(he)15%

 植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)磷(lin)(lin)脂酸(suan)（PA）酶(mei)聯免(mian)(mian)疫(yi)吸(xi)(xi)附測(ce)(ce)(ce)(ce)定(ding)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)僅(jin)供(gong)(gong)體(ti)(ti)(ti)(ti)外(wai)研究(jiu)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)，不(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)臨床(chuang)診斷(duan) 使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)說(shuo)(shuo) 明(ming)書   l 本(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)萃取(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)或(huo)其(qi)(qi)它(ta)相(xiang)關樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)磷(lin)(lin)脂酸(suan)（PA）的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)。l 有(you)效(xiao)期(qi)：6個(ge)月(yue)l 保(bao)存(cun)條件(jian)：2-8℃試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)組(zu)成(cheng)(cheng)：名(ming)稱96孔(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)(zhi)(zhi)48孔(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)(zhi)(zhi)備(bei)(bei)注(zhu)微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)12孔(kong)(kong)(kong)(kong)(kong)(kong)×8條12孔(kong)(kong)(kong)(kong)(kong)(kong)×4條無(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管無(wu)(wu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)稀(xi)(xi)(xi)(xi)釋液(ye)(ye)(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)抗(kang)體(ti)(ti)(ti)(ti)-HRP10mL5mL無(wu)(wu)20×洗(xi)(xi)滌(di)(di)緩(huan)(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)25mL15mL按(an)(an)說(shuo)(shuo)明(ming)書進(jin)行稀(xi)(xi)(xi)(xi)釋底(di)(di)(di)物(wu)(wu)(wu)(wu)A6mL3mL無(wu)(wu)底(di)(di)(di)物(wu)(wu)(wu)(wu)B6mL3mL無(wu)(wu)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)封(feng)(feng)(feng)板(ban)(ban)(ban)(ban)(ban)膜(mo)2張(zhang)2張(zhang)無(wu)(wu)說(shuo)(shuo)明(ming)書1份1份無(wu)(wu)自封(feng)(feng)(feng)袋1個(ge)1個(ge)無(wu)(wu)備(bei)(bei)注(zhu)：1. （96T/48T）打(da)開(kai)包(bao)裝后(hou)(hou)(hou)請(qing)及(ji)時(shi)(shi)檢(jian)(jian)(jian)查所(suo)(suo)有(you)物(wu)(wu)(wu)(wu)品(pin)(pin)(pin)(pin)(pin)(pin)是否齊全(quan)(quan)。2. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)依次(ci)(ci)(ci)為(wei)：100、50、25、12.5、6.25、0 nmol/L3. 經過(guo)大(da)量(liang)(liang)正(zheng)(zheng)常(chang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)驗(yan)(yan)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正(zheng)(zheng)常(chang)濃(nong)(nong)度(du)(du)(du)值(zhi)(zhi)均在(zai)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)提(ti)(ti)供(gong)(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)范(fan)(fan)圍(wei)內(nei)(nei)，實(shi)驗(yan)(yan)過(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)直(zhi)接(jie)取(qu)(qu)(qu)(qu)50μL樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)上(shang)(shang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)即(ji)(ji)(ji)可(ke)(ke)(ke)(ke)。當(dang)有(you)部(bu)分(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)值(zhi)(zhi)超過(guo)＊大(da)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)時(shi)(shi)，可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)稀(xi)(xi)(xi)(xi)釋液(ye)(ye)(ye)(ye)(ye)(ye)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)進(jin)行適當(dang)稀(xi)(xi)(xi)(xi)釋后(hou)(hou)(hou)再進(jin)行實(shi)驗(yan)(yan)。檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)前(qian)準(zhun)(zhun)備(bei)(bei)工(gong)作(zuo)：1. 請(qing)提(ti)(ti)前(qian)20分(fen)(fen)(fen)(fen)鐘從冰(bing)箱(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)取(qu)(qu)(qu)(qu)出(chu)(chu)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)，平(ping)(ping)衡至室(shi)溫(wen)(wen)(wen)(wen)(wen)。2. 從冰(bing)箱(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)取(qu)(qu)(qu)(qu)出(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)縮(suo)洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)可(ke)(ke)(ke)(ke)能(neng)(neng)有(you)結(jie)(jie)晶，屬于(yu)正(zheng)(zheng)常(chang)現象(xiang)；放(fang)(fang)置(zhi)(zhi)(zhi)室(shi)溫(wen)(wen)(wen)(wen)(wen)，輕搖均勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)，待結(jie)(jie)晶完(wan)全(quan)(quan)溶解后(hou)(hou)(hou)再配置(zhi)(zhi)(zhi)洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)。可(ke)(ke)(ke)(ke)將(jiang)(jiang)20ml濃(nong)(nong)縮(suo)洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)用(yong)(yong)(yong)(yong)(yong)(yong)蒸(zheng)餾(liu)(liu)水(shui)(shui)或(huo)去(qu)(qu)離子水(shui)(shui)稀(xi)(xi)(xi)(xi)釋配置(zhi)(zhi)(zhi)成(cheng)(cheng)400ml工(gong)作(zuo)濃(nong)(nong)度(du)(du)(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)，未用(yong)(yong)(yong)(yong)(yong)(yong)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)放(fang)(fang)回4℃3. 20×洗(xi)(xi)滌(di)(di)緩(huan)(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋：蒸(zheng)餾(liu)(liu)水(shui)(shui)按(an)(an)1：20稀(xi)(xi)(xi)(xi)釋，即(ji)(ji)(ji)1份20×洗(xi)(xi)滌(di)(di)緩(huan)(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)加(jia)(jia)(jia)(jia)19份蒸(zheng)餾(liu)(liu)水(shui)(shui)。實(shi)驗(yan)(yan)原理(li)(li)(li)：試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)采用(yong)(yong)(yong)(yong)(yong)(yong)雙(shuang)抗(kang)體(ti)(ti)(ti)(ti)一(yi)(yi)步夾心(xin)法酶(mei)聯免(mian)(mian)疫(yi)吸(xi)(xi)附試(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)（ELISA）。往預先包(bao)被(bei)植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)磷(lin)(lin)脂酸(suan)（PA）捕獲抗(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包(bao)被(bei)微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，依次(ci)(ci)(ci)加(jia)(jia)(jia)(jia)入(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)抗(kang)體(ti)(ti)(ti)(ti)，經過(guo)溫(wen)(wen)(wen)(wen)(wen)育(yu)并徹底(di)(di)(di)洗(xi)(xi)滌(di)(di)。用(yong)(yong)(yong)(yong)(yong)(yong)底(di)(di)(di)物(wu)(wu)(wu)(wu)TMB顯(xian)色(se)(se)(se)(se)，TMB在(zai)過(guo)氧(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催(cui)化(hua)(hua)下轉(zhuan)化(hua)(hua)成(cheng)(cheng)藍色(se)(se)(se)(se)，并在(zai)酸(suan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)下轉(zhuan)化(hua)(hua)成(cheng)(cheng)＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)色(se)(se)(se)(se)。顏色(se)(se)(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)磷(lin)(lin)脂酸(suan)（PA）呈(cheng)正(zheng)(zheng)相(xiang)關。用(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)在(zai)450nm 波長下測(ce)(ce)(ce)(ce)定(ding)吸(xi)(xi)光(guang)度(du)(du)(du)（OD 值(zhi)(zhi)），計(ji)算樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)含量(liang)(liang)。實(shi)驗(yan)(yan)所(suo)(suo)需(xu)自備(bei)(bei)試(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)器材：1. 酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)（450nm）2. 高精度(du)(du)(du)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)器及(ji)槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒(heng)溫(wen)(wen)(wen)(wen)(wen)箱(xiang)4. 蒸(zheng)餾(liu)(liu)水(shui)(shui)或(huo)去(qu)(qu)離子水(shui)(shui)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)處理(li)(li)(li)及(ji)要(yao)(yao)求：植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)組(zu)織(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)ELISA檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)處理(li)(li)(li)方法一(yi)(yi)、組(zu)織(zhi)(zhi)勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)(zhi)(zhi)備(bei)(bei)： 1、取(qu)(qu)(qu)(qu)組(zu)織(zhi)(zhi)塊(kuai)（0.1g-0.5g）＊少可(ke)(ke)(ke)(ke)到(dao)5-10mg在(zai)冰(bing)冷(leng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)PBS中(zhong)(zhong)(zhong)(zhong)(zhong)漂洗(xi)(xi)，濾(lv)紙(zhi)拭干(gan)，準(zhun)(zhun)確(que)(que)(que)稱重(zhong)，放(fang)(fang)入(ru)5ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)管中(zhong)(zhong)(zhong)(zhong)(zhong)。 2、按(an)(an)重(zhong)量(liang)(liang)(g):體(ti)(ti)(ti)(ti)積(ml)=1:9的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例加(jia)(jia)(jia)(jia)入(ru)9倍體(ti)(ti)(ti)(ti)積的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)介質(zhi)于(yu)勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)管中(zhong)(zhong)(zhong)(zhong)(zhong)，冰(bing)水(shui)(shui)浴(yu)條件(jian)下,用(yong)(yong)(yong)(yong)(yong)(yong)眼(yan)科(ke)小(xiao)(xiao)剪盡(jin)快剪碎組(zu)織(zhi)(zhi)塊(kuai)。 3、勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方式：手(shou)(shou)工(gong)勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)，機(ji)器勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)。 ① 手(shou)(shou)工(gong)勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)：左(zuo)手(shou)(shou)持勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)管將(jiang)(jiang)下端插入(ru)盛有(you)冰(bing)水(shui)(shui)混(hun)(hun)合(he)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)器皿中(zhong)(zhong)(zhong)(zhong)(zhong)，右手(shou)(shou)將(jiang)(jiang)搗桿垂直(zhi)插入(ru)套管中(zhong)(zhong)(zhong)(zhong)(zhong)，上(shang)(shang)下轉(zhuan)動研磨數(shu)(shu)十次(ci)(ci)(ci)（6-8分(fen)(fen)(fen)(fen)鐘），充(chong)分(fen)(fen)(fen)(fen)研碎，制(zhi)(zhi)(zhi)成(cheng)(cheng)10%的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)液(ye)(ye)(ye)(ye)(ye)(ye)。 ② 機(ji)器勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)：用(yong)(yong)(yong)(yong)(yong)(yong)組(zu)織(zhi)(zhi)搗碎機(ji)10000-15000 轉(zhuan)/分(fen)(fen)(fen)(fen)上(shang)(shang)下研磨制(zhi)(zhi)(zhi)成(cheng)(cheng)10%組(zu)織(zhi)(zhi)勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)，也可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)內(nei)(nei)切(qie)式組(zu)織(zhi)(zhi)勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)機(ji)制(zhi)(zhi)(zhi)備(bei)(bei)（勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)時(shi)(shi)間(jian)10秒(miao)/次(ci)(ci)(ci)，間(jian)隙30秒(miao)，連續3-5次(ci)(ci)(ci)，在(zai)冰(bing)水(shui)(shui)中(zhong)(zhong)(zhong)(zhong)(zhong)進(jin)行,可(ke)(ke)(ke)(ke)適當(dang)延長勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)時(shi)(shi)間(jian)），鏡檢(jian)(jian)(jian)觀察。4、將(jiang)(jiang)制(zhi)(zhi)(zhi)備(bei)(bei)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)10%勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)液(ye)(ye)(ye)(ye)(ye)(ye)用(yong)(yong)(yong)(yong)(yong)(yong)普通(tong)離心(xin)機(ji)或(huo)低(di)溫(wen)(wen)(wen)(wen)(wen)低(di)速離心(xin)機(ji)3000轉(zhuan)/分(fen)(fen)(fen)(fen)左(zuo)右,離心(xin)10-15分(fen)(fen)(fen)(fen)鐘，取(qu)(qu)(qu)(qu)上(shang)(shang)清液(ye)(ye)(ye)(ye)(ye)(ye)進(jin)行測(ce)(ce)(ce)(ce)定(ding)。二(er)、勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)漿(jiang)(jiang)(jiang)介質(zhi)： 一(yi)(yi)般(ban)采用(yong)(yong)(yong)(yong)(yong)(yong)0.05 mol/L Tris-HCl, pH 7.4 磷(lin)(lin)酸(suan)鹽(yan)緩(huan)(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)(PBS),客戶可(ke)(ke)(ke)(ke)根(gen)據樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)及(ji)測(ce)(ce)(ce)(ce)定(ding)指標(biao)(biao)(biao)(biao)(biao)(biao)(biao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情(qing)況自行設(she)定(ding)濃(nong)(nong)度(du)(du)(du),目(mu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是保(bao)持樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)等(deng)滲環(huan)境。三、樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)保(bao)存(cun)：       1. 植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)組(zu)織(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)暫(zan)時(shi)(shi)不(bu)(bu)(bu)測(ce)(ce)(ce)(ce)定(ding)，可(ke)(ke)(ke)(ke)立即(ji)(ji)(ji)低(di)溫(wen)(wen)(wen)(wen)(wen)凍(dong)存(cun)，溫(wen)(wen)(wen)(wen)(wen)度(du)(du)(du)越低(di)越好，中(zhong)(zhong)(zhong)(zhong)(zhong)間(jian)如(ru)不(bu)(bu)(bu)反(fan)(fan)復凍(dong)融，-20℃以(yi)(yi)下可(ke)(ke)(ke)(ke)保(bao) 存(cun)三個(ge)月(yue),-70℃以(yi)(yi)下可(ke)(ke)(ke)(ke)保(bao)存(cun)六個(ge)月(yue)。注(zhu)意事項：1. 嚴格(ge)按(an)(an)照規定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)時(shi)(shi)間(jian)和(he)(he)溫(wen)(wen)(wen)(wen)(wen)度(du)(du)(du)進(jin)行溫(wen)(wen)(wen)(wen)(wen)育(yu)以(yi)(yi)保(bao)證準(zhun)(zhun)確(que)(que)(que)結(jie)(jie)果(guo)。所(suo)(suo)有(you)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)都必須在(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)達到(dao)室(shi)溫(wen)(wen)(wen)(wen)(wen)20-25℃。使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)立即(ji)(ji)(ji)冷(leng)藏保(bao)存(cun)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)。2. 洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)不(bu)(bu)(bu)正(zheng)(zheng)確(que)(que)(que)可(ke)(ke)(ke)(ke)以(yi)(yi)導致(zhi)不(bu)(bu)(bu)準(zhun)(zhun)確(que)(que)(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)(jie)果(guo)。在(zai)加(jia)(jia)(jia)(jia)入(ru)底(di)(di)(di)物(wu)(wu)(wu)(wu)前(qian)確(que)(que)(que)保(bao)盡(jin)量(liang)(liang)吸(xi)(xi)干(gan)孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)。溫(wen)(wen)(wen)(wen)(wen)育(yu)過(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)不(bu)(bu)(bu)要(yao)(yao)讓微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)干(gan)燥掉。3. 消除板(ban)(ban)(ban)(ban)(ban)底(di)(di)(di)殘留的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)和(he)(he)手(shou)(shou)指印，否則影(ying)響OD值(zhi)(zhi)。4. 底(di)(di)(di)物(wu)(wu)(wu)(wu)顯(xian)色(se)(se)(se)(se)液(ye)(ye)(ye)(ye)(ye)(ye)應(ying)(ying)呈(cheng)無(wu)(wu)色(se)(se)(se)(se)或(huo)很淺的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)顏色(se)(se)(se)(se)，已(yi)經變(bian)(bian)藍的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)(di)物(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)不(bu)(bu)(bu)能(neng)(neng)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)。5. 避免(mian)(mian)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)和(he)(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)交(jiao)叉(cha)(cha)污染以(yi)(yi)免(mian)(mian)造成(cheng)(cheng)錯誤結(jie)(jie)果(guo)。6. 在(zai)儲存(cun)和(he)(he)溫(wen)(wen)(wen)(wen)(wen)育(yu)時(shi)(shi)避免(mian)(mian)強(qiang)光(guang)直(zhi)接(jie)照射。7. 平(ping)(ping)衡至室(shi)溫(wen)(wen)(wen)(wen)(wen)后(hou)(hou)(hou)再打(da)開(kai)密(mi)封(feng)(feng)(feng)袋以(yi)(yi)防(fang)水(shui)(shui)滴凝聚在(zai)冷(leng)板(ban)(ban)(ban)(ban)(ban)條上(shang)(shang)。8. 任(ren)何(he)反(fan)(fan)應(ying)(ying)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)不(bu)(bu)(bu)能(neng)(neng)接(jie)觸漂白(bai)(bai)溶劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)或(huo)漂白(bai)(bai)溶劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)所(suo)(suo)散發(fa)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)強(qiang)烈氣體(ti)(ti)(ti)(ti)。任(ren)何(he)漂白(bai)(bai)成(cheng)(cheng)分(fen)(fen)(fen)(fen)都會(hui)破壞試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)反(fan)(fan)應(ying)(ying)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)物(wu)(wu)(wu)(wu)活性。9. 不(bu)(bu)(bu)能(neng)(neng)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)過(guo)期(qi)產(chan)(chan)品(pin)(pin)(pin)(pin)(pin)(pin)。10. 如(ru)果(guo)可(ke)(ke)(ke)(ke)能(neng)(neng)傳播疾病，所(suo)(suo)有(you)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)都應(ying)(ying)管理(li)(li)(li)好，按(an)(an)照規定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)程序處理(li)(li)(li)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)和(he)(he)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)裝置(zhi)(zhi)(zhi)。操作(zuo)步驟：1．從室(shi)溫(wen)(wen)(wen)(wen)(wen)平(ping)(ping)衡60min后(hou)(hou)(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)鋁箔袋中(zhong)(zhong)(zhong)(zhong)(zhong)取(qu)(qu)(qu)(qu)出(chu)(chu)所(suo)(suo)需(xu)板(ban)(ban)(ban)(ban)(ban)條，剩(sheng)余板(ban)(ban)(ban)(ban)(ban)條用(yong)(yong)(yong)(yong)(yong)(yong)自封(feng)(feng)(feng)袋密(mi)封(feng)(feng)(feng)放(fang)(fang)回4℃。2．設(she)置(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)各加(jia)(jia)(jia)(jia)不(bu)(bu)(bu)同(tong)濃(nong)(nong)度(du)(du)(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)50μL。3．樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)入(ru)待測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)50μL；空白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)加(jia)(jia)(jia)(jia)。樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)不(bu)(bu)(bu)足(zu)，或(huo)者(zhe)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)濃(nong)(nong)度(du)(du)(du)過(guo)高，可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)稀(xi)(xi)(xi)(xi)釋液(ye)(ye)(ye)(ye)(ye)(ye)做稀(xi)(xi)(xi)(xi)釋。4.  除空白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)外(wai)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)每孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)辣根(gen)過(guo)氧(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)（HRP）標(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)抗(kang)體(ti)(ti)(ti)(ti)100μL，用(yong)(yong)(yong)(yong)(yong)(yong)封(feng)(feng)(feng)板(ban)(ban)(ban)(ban)(ban)膜(mo)封(feng)(feng)(feng)住反(fan)(fan)應(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)，37℃水(shui)(shui)浴(yu)鍋或(huo)恒(heng)溫(wen)(wen)(wen)(wen)(wen)箱(xiang)溫(wen)(wen)(wen)(wen)(wen)育(yu)60min。5．棄去(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，吸(xi)(xi)水(shui)(shui)紙(zhi)上(shang)(shang)拍(pai)干(gan)，每孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)（350μL），靜置(zhi)(zhi)(zhi)1min，甩(shuai)去(qu)(qu)洗(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)，吸(xi)(xi)水(shui)(shui)紙(zhi)上(shang)(shang)拍(pai)干(gan)，如(ru)此重(zhong)復洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)5次(ci)(ci)(ci)（也可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)機(ji)洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)）。6.  每孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)底(di)(di)(di)物(wu)(wu)(wu)(wu)A、B各50μL，37℃避光(guang)孵育(yu)15min。7.  每孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)50μL，15min內(nei)(nei)，在(zai)450nm波長處測(ce)(ce)(ce)(ce)定(ding)各孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)。實(shi)驗(yan)(yan)結(jie)(jie)果(guo)計(ji)算：繪制(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)線：以(yi)(yi)所(suo)(suo)測(ce)(ce)(ce)(ce)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)為(wei)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)值(zhi)(zhi)為(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)或(huo)用(yong)(yong)(yong)(yong)(yong)(yong)相(xiang)關軟件(jian)繪制(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)線，并得到(dao)直(zhi)線回歸方程，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)代(dai)入(ru)方程，計(ji)算出(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)。  （此圖僅(jin)供(gong)(gong)參考）試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)性能(neng)(neng)：1. 檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)范(fan)(fan)圍(wei)：1.95 nmol/L –100 nmol/L。2. 靈敏(min)度(du)(du)(du)：＊低(di)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)濃(nong)(nong)度(du)(du)(du)小(xiao)(xiao)于(yu)0.78 nmol/L。3. 特異(yi)性：不(bu)(bu)(bu)與其(qi)(qi)它(ta)可(ke)(ke)(ke)(ke)溶性結(jie)(jie)構類似物(wu)(wu)(wu)(wu)交(jiao)叉(cha)(cha)反(fan)(fan)應(ying)(ying)。4. 重(zhong)復性：板(ban)(ban)(ban)(ban)(ban)內(nei)(nei)變(bian)(bian)異(yi)系(xi)數(shu)(shu)小(xiao)(xiao)于(yu)10% ，板(ban)(ban)(ban)(ban)(ban)間(jian)變(bian)(bian)異(yi)系(xi)數(shu)(shu)小(xiao)(xiao)于(yu)15% 。技(ji)術小(xiao)(xiao)提(ti)(ti)示：1. 當(dang)混(hun)(hun)合(he)或(huo)重(zhong)溶蛋白(bai)(bai)溶液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)，盡(jin)量(liang)(liang)避免(mian)(mian)起沫。2. 為(wei)了避免(mian)(mian)交(jiao)叉(cha)(cha)感染，配置(zhi)(zhi)(zhi)不(bu)(bu)(bu)同(tong)濃(nong)(nong)度(du)(du)(du)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、上(shang)(shang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)、加(jia)(jia)(jia)(jia)不(bu)(bu)(bu)同(tong)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)都需(xu)要(yao)(yao)更換槍頭。另外(wai)不(bu)(bu)(bu)同(tong)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)請(qing)分(fen)(fen)(fen)(fen)別使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)同(tong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)移液(ye)(ye)(ye)(ye)(ye)(ye)槽。3. 每次(ci)(ci)(ci)孵育(yu)時(shi)(shi)，請(qing)正(zheng)(zheng)確(que)(que)(que)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)封(feng)(feng)(feng)板(ban)(ban)(ban)(ban)(ban)膠可(ke)(ke)(ke)(ke)保(bao)證結(jie)(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)確(que)(que)(que)性。4. 混(hun)(hun)合(he)后(hou)(hou)(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)顯(xian)色(se)(se)(se)(se)底(di)(di)(di)物(wu)(wu)(wu)(wu)在(zai)上(shang)(shang)板(ban)(ban)(ban)(ban)(ban)前(qian)應(ying)(ying)為(wei)無(wu)(wu)色(se)(se)(se)(se)，請(qing)避光(guang)保(bao)存(cun)；加(jia)(jia)(jia)(jia)入(ru)微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)板(ban)(ban)(ban)(ban)(ban)后(hou)(hou)(hou)，將(jiang)(jiang)由無(wu)(wu)色(se)(se)(se)(se)變(bian)(bian)成(cheng)(cheng)不(bu)(bu)(bu)同(tong)深度(du)(du)(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)藍色(se)(se)(se)(se)。5. 終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)上(shang)(shang)板(ban)(ban)(ban)(ban)(ban)順序應(ying)(ying)同(tong)顯(xian)色(se)(se)(se)(se)底(di)(di)(di)物(wu)(wu)(wu)(wu)上(shang)(shang)板(ban)(ban)(ban)(ban)(ban)順序一(yi)(yi)致(zhi)；加(jia)(jia)(jia)(jia)入(ru)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)，孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)顏色(se)(se)(se)(se)由藍變(bian)(bian)黃(huang)；若(ruo)孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)有(you)綠色(se)(se)(se)(se)，則表明(ming)孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)未混(hun)(hun)勻(yun)(yun)(yun)(yun)(yun)(yun)(yun)；請(qing)充(chong)分(fen)(fen)(fen)(fen)混(hun)(hun)合(he)。說(shuo)(shuo)明(ming)：1. 由于(yu)現有(you)條件(jian)及(ji)科(ke)學(xue)技(ji)術水(shui)(shui)平(ping)(ping)尚不(bu)(bu)(bu)能(neng)(neng)對所(suo)(suo)有(you)供(gong)(gong)貨商提(ti)(ti)供(gong)(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)所(suo)(suo)有(you)原料進(jin)行全(quan)(quan)面(mian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)鑒定(ding)與分(fen)(fen)(fen)(fen)析，本(ben)(ben)(ben)(ben)(ben)產(chan)(chan)品(pin)(pin)(pin)(pin)(pin)(pin)可(ke)(ke)(ke)(ke)能(neng)(neng)存(cun)在(zai)一(yi)(yi)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)量(liang)(liang)技(ji)術風險。2. ＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗(yan)(yan)結(jie)(jie)果(guo)與試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)效(xiao)性、實(shi)驗(yan)(yan)者(zhe)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)相(xiang)關操作(zuo)以(yi)(yi)及(ji)當(dang)時(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗(yan)(yan)環(huan)境密(mi)切(qie)相(xiang)關，請(qing)務必準(zhun)(zhun)備(bei)(bei)充(chong)足(zu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)備(bei)(bei)份。3. 不(bu)(bu)(bu)同(tong)批(pi)次(ci)(ci)(ci)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)一(yi)(yi)產(chan)(chan)品(pin)(pin)(pin)(pin)(pin)(pin)可(ke)(ke)(ke)(ke)能(neng)(neng)會(hui)有(you)少許差別，如(ru)：檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)限(xian)、靈敏(min)度(du)(du)(du)以(yi)(yi)及(ji)顯(xian)色(se)(se)(se)(se)時(shi)(shi)間(jian)等(deng)，請(qing)依據試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)內(nei)(nei)說(shuo)(shuo)明(ming)書進(jin)行實(shi)驗(yan)(yan)操作(zuo)，網站電子版說(shuo)(shuo)明(ming)書僅(jin)作(zuo)參考。4. 只(zhi)有(you)全(quan)(quan)部(bu)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)本(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)配套試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)才能(neng)(neng)保(bao)證檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)效(xiao)果(guo)，不(bu)(bu)(bu)能(neng)(neng)混(hun)(hun)用(yong)(yong)(yong)(yong)(yong)(yong)其(qi)(qi)他制(zhi)(zhi)(zhi)造商的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)(chan)品(pin)(pin)(pin)(pin)(pin)(pin)。只(zhi)有(you)嚴格(ge)遵守本(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗(yan)(yan)說(shuo)(shuo)明(ming)才會(hui)得到(dao)＊佳的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)結(jie)(jie)果(guo)。 

實(shi)(shi)(shi)驗(yan)原理(li)   試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)采用(yong)(yong)(yong)雙(shuang)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)一(yi)(yi)(yi)步夾心(xin)法酶(mei)(mei)(mei)聯(lian)免(mian)疫吸(xi)附試(shi)(shi)(shi)(shi)驗(yan)（ELISA）。往預(yu)先包(bao)被硝(xiao)酸(suan)(suan)還(huan)原酶(mei)(mei)(mei)(NR)捕獲抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包(bao)被微孔(kong)中(zhong)(zhong)，依(yi)(yi)次(ci)(ci)(ci)加(jia)(jia)(jia)入標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢測(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)，經過(guo)(guo)(guo)(guo)(guo)溫(wen)育(yu)并徹(che)底(di)洗(xi)(xi)滌(di)。用(yong)(yong)(yong)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯(xian)色(se)(se)，TMB在(zai)(zai)過(guo)(guo)(guo)(guo)(guo)氧化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)下(xia)轉化(hua)(hua)成(cheng)藍(lan)色(se)(se)，并在(zai)(zai)酸(suan)(suan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)下(xia)轉化(hua)(hua)成(cheng)＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)(se)。顏色(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺(qian)和樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)硝(xiao)酸(suan)(suan)還(huan)原酶(mei)(mei)(mei)(NR)呈正相(xiang)(xiang)關。用(yong)(yong)(yong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)450nm 波(bo)長下(xia)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)吸(xi)光度(du)(du)(du)(du)（OD 值(zhi)(zhi)(zhi)），計(ji)(ji)(ji)(ji)算(suan)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)。保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)條(tiao)件(jian)及(ji)有(you)效(xiao)期1.本(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)用(yong)(yong)(yong)于(yu)(yu)(yu)體(ti)(ti)(ti)(ti)(ti)外定(ding)(ding)(ding)量(liang)(liang)檢測(ce)(ce)(ce)(ce)(ce)血(xue)清(qing)(qing)、血(xue)漿、組織(zhi)勻(yun)(yun)漿及(ji)相(xiang)(xiang)關液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)硝(xiao)酸(suan)(suan)還(huan)原酶(mei)(mei)(mei)(NR)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)；2.試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)：2-8℃；3.有(you)效(xiao)期：6個月。植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)硝(xiao)酸(suan)(suan)還(huan)原酶(mei)(mei)(mei)(NR)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采集及(ji)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)1.血(xue)清(qing)(qing)：全血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)請(qing)(qing)(qing)(qing)(qing)于(yu)(yu)(yu)室溫(wen)放(fang)置(zhi)2小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)或(huo)4℃過(guo)(guo)(guo)(guo)(guo)一(yi)(yi)(yi)晚后(hou)(hou)(hou)(hou)(hou)(hou)于(yu)(yu)(yu)1000 x g離心(xin)20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，取上(shang)(shang)(shang)清(qing)(qing)即(ji)(ji)可(ke)(ke)(ke)檢測(ce)(ce)(ce)(ce)(ce)，或(huo)將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)-20℃或(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)，但應(ying)(ying)(ying)避免(mian)反(fan)(fan)復凍融(rong)。 2.血(xue)漿：可(ke)(ke)(ke)用(yong)(yong)(yong)EDTA或(huo)肝(gan)素(su)(su)(su)(su)作(zuo)(zuo)為(wei)(wei)(wei)(wei)抗(kang)(kang)(kang)凝劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)采集后(hou)(hou)(hou)(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)于(yu)(yu)(yu)2 - 8° C 1000 x g離心(xin)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，或(huo)將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)-20℃或(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)，但應(ying)(ying)(ying)避免(mian)反(fan)(fan)復凍融(rong)。 3.細胞培養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)清(qing)(qing)或(huo)其(qi)(qi)它生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)：1000 x g離心(xin)20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，取上(shang)(shang)(shang)清(qing)(qing)即(ji)(ji)可(ke)(ke)(ke)檢測(ce)(ce)(ce)(ce)(ce)，或(huo)將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)-20℃或(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)，但應(ying)(ying)(ying)避免(mian)反(fan)(fan)復凍融(rong)。 (注(zhu)(zhu)(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)溶(rong)(rong)血(xue)會影響**檢測(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)(guo)，因(yin)此溶(rong)(rong)血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)不(bu)(bu)(bu)宜(yi)進(jin)(jin)行(xing)此項檢測(ce)(ce)(ce)(ce)(ce)。) 植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)硝(xiao)酸(suan)(suan)還(huan)原酶(mei)(mei)(mei)(NR)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)   標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則(ze)(ze)：首先通(tong)過(guo)(guo)(guo)(guo)(guo)文獻檢索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)式(shi)(shi)(shi)了解(jie)待(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含量(liang)(liang)，確定(ding)(ding)(ding)適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)。只有(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍內(nei)(nei)(nei)(nei)，檢測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果(guo)(guo)才(cai)是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)中(zhong)(zhong)，應(ying)(ying)(ying)做(zuo)好(hao)詳(xiang)細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)(ji)(ji)錄。計(ji)(ji)(ji)(ji)算(suan)濃(nong)(nong)(nong)度(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)應(ying)(ying)(ying)再乘(cheng)以(yi)(yi)“N”。    標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則(ze)(ze)：2瓶，每瓶臨(lin)用(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)1ml，蓋(gai)(gai)好(hao)后(hou)(hou)(hou)(hou)(hou)(hou)靜置(zhi)10分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)上(shang)(shang)(shang)，然后(hou)(hou)(hou)(hou)(hou)(hou)反(fan)(fan)復顛倒(dao)/搓(cuo)動以(yi)(yi)助溶(rong)(rong)解(jie)，其(qi)(qi)濃(nong)(nong)(nong)度(du)(du)(du)(du)為(wei)(wei)(wei)(wei)300 pg/ml，做(zuo)系列(lie)倍(bei)(bei)(bei)比稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)直接作(zuo)(zuo)為(wei)(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)度(du)(du)(du)(du)0 pg/ml，臨(lin)用(yong)(yong)(yong)前(qian)(qian)(qian)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。 如(ru)(ru)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取0.5ml（不(bu)(bu)(bu)要(yao)少于(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)入含0.5ml樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中(zhong)(zhong)，混(hun)勻(yun)(yun)即(ji)(ji)可(ke)(ke)(ke)，其(qi)(qi)余濃(nong)(nong)(nong)度(du)(du)(du)(du)以(yi)(yi)此類(lei)推。    生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則(ze)(ze)：臨(lin)用(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)(gen)(gen)據預(yu)先計(ji)(ji)(ji)(ji)算(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每次(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)（每孔(kong)100μl），實(shi)(shi)(shi)際(ji)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)加(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，輕輕混(hun)勻(yun)(yun)，在(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)(yi)(yi)小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。    辣(la)根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和素(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則(ze)(ze)：臨(lin)用(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)辣(la)根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和素(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)(gen)(gen)據預(yu)先計(ji)(ji)(ji)(ji)算(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每次(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)（每孔(kong)100μl），實(shi)(shi)(shi)際(ji)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl辣(la)根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和素(su)(su)(su)(su)加(jia)(jia)(jia)990μl辣(la)根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和素(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，輕輕混(hun)勻(yun)(yun)，在(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)(yi)(yi)小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。 植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)硝(xiao)酸(suan)(suan)還(huan)原酶(mei)(mei)(mei)(NR)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)操(cao)作(zuo)(zuo)步驟   實(shi)(shi)(shi)驗(yan)開(kai)始前(qian)(qian)(qian)，請(qing)(qing)(qing)(qing)(qing)提前(qian)(qian)(qian)配(pei)(pei)(pei)(pei)置(zhi)好(hao)所有(you)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)或(huo)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)，均需混(hun)勻(yun)(yun)，混(hun)勻(yun)(yun)時(shi)(shi)(shi)(shi)(shi)(shi)盡(jin)量(liang)(liang)避免(mian)起泡(pao)。每次(ci)(ci)(ci)檢測(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)該做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線。如(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)過(guo)(guo)(guo)(guo)(guo)高(gao)時(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)進(jin)(jin)行(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)使(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)符(fu)合試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢測(ce)(ce)(ce)(ce)(ce)范圍。 1.加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)別(bie)設空(kong)白孔(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)、待(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)。空(kong)白孔(kong)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)分(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)待(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)(zhu)(zhu)意不(bu)(bu)(bu)要(yao)有(you)氣(qi)泡(pao)，加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)將樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)于(yu)(yu)(yu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)底(di)部，盡(jin)量(liang)(liang)不(bu)(bu)(bu)觸及(ji)孔(kong)壁(bi)，輕輕晃動混(hun)勻(yun)(yun)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)(jia)上(shang)(shang)(shang)蓋(gai)(gai)或(huo)覆膜，37℃反(fan)(fan)應(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)(wei)保(bao)(bao)(bao)(bao)證(zheng)實(shi)(shi)(shi)驗(yan)結(jie)果(guo)(guo)有(you)效(xiao)性(xing)(xing)，每次(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)請(qing)(qing)(qing)(qing)(qing)使(shi)(shi)用(yong)(yong)(yong)新(xin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 2.棄(qi)(qi)去(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩(shuai)干(gan)，不(bu)(bu)(bu)用(yong)(yong)(yong)洗(xi)(xi)滌(di)。每孔(kong)加(jia)(jia)(jia)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)加(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，輕輕混(hun)勻(yun)(yun)，在(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)(yi)(yi)小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 3.溫(wen)育(yu)60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)(hou)(hou)，棄(qi)(qi)去(qu)孔(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩(shuai)干(gan)，洗(xi)(xi)板(ban)(ban)(ban)3次(ci)(ci)(ci)，每次(ci)(ci)(ci)浸(jin)泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每孔(kong)，甩(shuai)干(gan)。4.每孔(kong)加(jia)(jia)(jia)辣(la)根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和素(su)(su)(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同(tong)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。5.溫(wen)育(yu)60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)(hou)(hou)，棄(qi)(qi)去(qu)孔(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩(shuai)干(gan)，洗(xi)(xi)板(ban)(ban)(ban)5次(ci)(ci)(ci)，每次(ci)(ci)(ci)浸(jin)泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每孔(kong)，甩(shuai)干(gan)。6.依(yi)(yi)序(xu)每孔(kong)加(jia)(jia)(jia)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避光顯(xian)色(se)(se)（30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)，此時(shi)(shi)(shi)(shi)(shi)(shi)肉眼可(ke)(ke)(ke)見(jian)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)3-4孔(kong)有(you)明(ming)顯(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)(du)藍(lan)色(se)(se)，后(hou)(hou)(hou)(hou)(hou)(hou)3-4孔(kong)梯度(du)(du)(du)(du)不(bu)(bu)(bu)明(ming)顯(xian)，即(ji)(ji)可(ke)(ke)(ke)終(zhong)止）。7.依(yi)(yi)序(xu)每孔(kong)加(jia)(jia)(jia)終(zhong)止溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止反(fan)(fan)應(ying)(ying)(ying)（此時(shi)(shi)(shi)(shi)(shi)(shi)藍(lan)色(se)(se)立轉黃色(se)(se)）。終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)入順序(xu)應(ying)(ying)(ying)盡(jin)量(liang)(liang)與底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)入順序(xu)相(xiang)(xiang)同(tong)。為(wei)(wei)(wei)(wei)了保(bao)(bao)(bao)(bao)證(zheng)實(shi)(shi)(shi)驗(yan)結(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)，底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)(fan)應(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)間(jian)(jian)到(dao)后(hou)(hou)(hou)(hou)(hou)(hou)應(ying)(ying)(ying)盡(jin)快加(jia)(jia)(jia)入終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)酶(mei)(mei)(mei)聯(lian)儀在(zai)(zai)450nm波(bo)長依(yi)(yi)序(xu)測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)各(ge)(ge)孔(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光密度(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)）。 在(zai)(zai)加(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)內(nei)(nei)(nei)(nei)進(jin)(jin)行(xing)檢測(ce)(ce)(ce)(ce)(ce)。植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)硝(xiao)酸(suan)(suan)還(huan)原酶(mei)(mei)(mei)(NR)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)注(zhu)(zhu)(zhu)意事項1.用(yong)(yong)(yong)戶在(zai)(zai)初次(ci)(ci)(ci)使(shi)(shi)用(yong)(yong)(yong)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)時(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)將各(ge)(ge)種試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)管離心(xin)數(shu)(shu)分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，以(yi)(yi)便試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)集中(zhong)(zhong)到(dao)管底(di)。2.每次(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)留一(yi)(yi)(yi)孔(kong)作(zuo)(zuo)為(wei)(wei)(wei)(wei)空(kong)白調(diao)零(ling)(ling)孔(kong)，該孔(kong)不(bu)(bu)(bu)加(jia)(jia)(jia)任何試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，只是**加(jia)(jia)(jia)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)及(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)時(shi)(shi)(shi)(shi)(shi)(shi)先用(yong)(yong)(yong)此孔(kong)調(diao)OD值(zhi)(zhi)(zhi)至(zhi)零(ling)(ling)。 3.為(wei)(wei)(wei)(wei)防止樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)(shi)驗(yan)時(shi)(shi)(shi)(shi)(shi)(shi)將反(fan)(fan)應(ying)(ying)(ying)板(ban)(ban)(ban)放(fang)于(yu)(yu)(yu)鋪(pu)有(you)濕布(bu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)(he)(he)內(nei)(nei)(nei)(nei)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)(jia)上(shang)(shang)(shang)蓋(gai)(gai)或(huo)覆膜。 4.未使(shi)(shi)用(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)或(huo)者試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，請(qing)(qing)(qing)(qing)(qing)于(yu)(yu)(yu)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和素(su)(su)(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)請(qing)(qing)(qing)(qing)(qing)依(yi)(yi)據所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)配(pei)(pei)(pei)(pei)置(zhi)使(shi)(shi)用(yong)(yong)(yong)。請(qing)(qing)(qing)(qing)(qing)勿重復使(shi)(shi)用(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)、辣(la)根(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和素(su)(su)(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5.建議檢測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)均設雙(shuang)孔(kong)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，以(yi)(yi)保(bao)(bao)(bao)(bao)證(zheng)檢測(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)。 植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)硝(xiao)酸(suan)(suan)還(huan)原酶(mei)(mei)(mei)(NR)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)洗(xi)(xi)板(ban)(ban)(ban)方(fang)(fang)法手工(gong)(gong)(gong)洗(xi)(xi)板(ban)(ban)(ban)方(fang)(fang)法：吸(xi)去(qu)（不(bu)(bu)(bu)可(ke)(ke)(ke)觸及(ji)板(ban)(ban)(ban)壁(bi)）或(huo)甩(shuai)掉酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)內(nei)(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)；在(zai)(zai)實(shi)(shi)(shi)驗(yan)臺上(shang)(shang)(shang)鋪(pu)墊幾(ji)層吸(xi)水紙(zhi)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)朝下(xia)用(yong)(yong)(yong)力拍幾(ji)次(ci)(ci)(ci)；將推薦(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)緩(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)至(zhi)少0.3ml注(zhu)(zhu)(zhu)入孔(kong)內(nei)(nei)(nei)(nei)，浸(jin)泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。根(gen)(gen)(gen)據需要(yao)，重復此過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)數(shu)(shu)次(ci)(ci)(ci)。 自動洗(xi)(xi)板(ban)(ban)(ban)：如(ru)(ru)果(guo)(guo)有(you)自動洗(xi)(xi)板(ban)(ban)(ban)機，應(ying)(ying)(ying)在(zai)(zai)熟練使(shi)(shi)用(yong)(yong)(yong)后(hou)(hou)(hou)(hou)(hou)(hou)再用(yong)(yong)(yong)到(dao)正式(shi)(shi)(shi)實(shi)(shi)(shi)驗(yan)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)中(zhong)(zhong)。 計(ji)(ji)(ji)(ji)算(suan)   以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)為(wei)(wei)(wei)(wei)橫(heng)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對(dui)數(shu)(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱(zong)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通(tong)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)半對(dui)數(shu)(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)(shang)繪(hui)出(chu)(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線，根(gen)(gen)(gen)據樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線查出(chu)(chu)(chu)(chu)相(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)；再乘(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)；或(huo)用(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)與OD值(zhi)(zhi)(zhi)計(ji)(ji)(ji)(ji)算(suan)出(chu)(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線回(hui)歸方(fang)(fang)程(cheng)(cheng)(cheng)式(shi)(shi)(shi)，將樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入方(fang)(fang)程(cheng)(cheng)(cheng)式(shi)(shi)(shi)，計(ji)(ji)(ji)(ji)算(suan)出(chu)(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)，再乘(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)，即(ji)(ji)為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際(ji)濃(nong)(nong)(nong)度(du)(du)(du)(du)。 注(zhu)(zhu)(zhu)意事項1.當混(hun)合蛋白溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)盡(jin)量(liang)(liang)輕緩(huan)，避免(mian)起泡(pao)。2.洗(xi)(xi)滌(di)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)非常重要(yao)，不(bu)(bu)(bu)充分(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)易造成(cheng)假陽性(xing)(xing)。3.一(yi)(yi)(yi)次(ci)(ci)(ci)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)間(jian)(jian)**控制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)在(zai)(zai)5分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)數(shu)(shu)量(liang)(liang)多，推薦(jian)使(shi)(shi)用(yong)(yong)(yong)排槍加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)。4.請(qing)(qing)(qing)(qing)(qing)每次(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi)(shi)(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線，做(zuo)復孔(kong)。5.如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)待(dai)測(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)(liang)過(guo)(guo)(guo)(guo)(guo)高(gao)，請(qing)(qing)(qing)(qing)(qing)先稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)(hou)再測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，計(ji)(ji)(ji)(ji)算(suan)時(shi)(shi)(shi)(shi)(shi)(shi)請(qing)(qing)(qing)(qing)(qing)乘(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)。6.在(zai)(zai)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢測(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)(qing)(qing)(qing)(qing)以(yi)(yi)相(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，不(bu)(bu)(bu)能混(hun)淆。7.底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)(qing)(qing)(qing)避光保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。 8.不(bu)(bu)(bu)要(yao)用(yong)(yong)(yong)其(qi)(qi)它生(sheng)(sheng)(sheng)產廠家(jia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)替(ti)換試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)。 注(zhu)(zhu)(zhu)意事項：收(shou)集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)前(qian)(qian)(qian)必(bi)須清(qing)(qing)楚要(yao)檢測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)份(fen)是否(fou)足(zu)夠穩定(ding)(ding)(ding)。對(dui)收(shou)集后(hou)(hou)(hou)(hou)(hou)(hou)當天進(jin)(jin)行(xing)檢測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)，儲(chu)存(cun)(cun)(cun)在(zai)(zai)4 ℃備用(yong)(yong)(yong)，如(ru)(ru)有(you)特殊原因(yin)需要(yao)周期收(shou)集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)， 將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)及(ji)時(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)裝后(hou)(hou)(hou)(hou)(hou)(hou)放(fang)在(zai)(zai)-20 ℃或(huo)-70 ℃條(tiao)件(jian)下(xia)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。避免(mian)反(fan)(fan)復凍融(rong)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)48 小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)1 個月。-70度(du)(du)(du)(du)可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)6 個月。部分(fen)(fen)(fen)(fen)激(ji)素(su)(su)(su)(su)類(lei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)需添加(jia)(jia)(jia)抑肽酶(mei)(mei)(mei)。計(ji)(ji)(ji)(ji)算(suan)：以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)為(wei)(wei)(wei)(wei)橫(heng)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱(zong)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)(shang)繪(hui)出(chu)(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線，根(gen)(gen)(gen)據樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線查出(chu)(chu)(chu)(chu)相(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)；再乘(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)；或(huo)用(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)與OD值(zhi)(zhi)(zhi)計(ji)(ji)(ji)(ji)算(suan)出(chu)(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線回(hui)歸方(fang)(fang)程(cheng)(cheng)(cheng)式(shi)(shi)(shi)，將樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入方(fang)(fang)程(cheng)(cheng)(cheng)式(shi)(shi)(shi)，計(ji)(ji)(ji)(ji)算(suan)出(chu)(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)，再乘(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)，即(ji)(ji)為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際(ji)濃(nong)(nong)(nong)度(du)(du)(du)(du)。 植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)硝(xiao)酸(suan)(suan)還(huan)原酶(mei)(mei)(mei)(NR)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)性(xing)(xing)能1.樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線性(xing)(xing)回(hui)歸與預(yu)期濃(nong)(nong)(nong)度(du)(du)(du)(du)相(xiang)(xiang)關系數(shu)(shu)R值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)0.92以(yi)(yi)上(shang)(shang)(shang)。2.批內(nei)(nei)(nei)(nei)與批見(jian)應(ying)(ying)(ying)分(fen)(fen)(fen)(fen)別(bie)小(xiao)于(yu)(yu)(yu)9%和15% 植(zhi)(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)硝(xiao)酸(suan)(suan)還(huan)原酶(mei)(mei)(mei)(NR)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)

實(shi)(shi)(shi)(shi)驗原(yuan)(yuan)(yuan)理   試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)采(cai)用(yong)(yong)(yong)(yong)(yong)雙(shuang)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)一(yi)步夾心法酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免疫吸(xi)附(fu)試(shi)(shi)(shi)(shi)(shi)(shi)驗（ELISA）。往預(yu)先包被(bei)(bei)Rubisco活(huo)(huo)化(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(mei)(RCA)捕獲抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被(bei)(bei)微孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，依(yi)(yi)(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)，經過(guo)(guo)溫(wen)育并徹底(di)(di)(di)洗(xi)(xi)滌(di)。用(yong)(yong)(yong)(yong)(yong)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯(xian)色，TMB在過(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)(hua)(hua)下(xia)轉(zhuan)化(hua)(hua)(hua)(hua)成藍(lan)(lan)色，并在酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)下(xia)轉(zhuan)化(hua)(hua)(hua)(hua)成＊終的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)色。顏色的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Rubisco活(huo)(huo)化(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(mei)(RCA)呈正(zheng)相(xiang)關。用(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在450nm 波長(chang)下(xia)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)吸(xi)光度(du)(du)(du)（OD 值(zhi)(zhi)(zhi)），計(ji)(ji)(ji)算樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)。保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)條(tiao)件(jian)(jian)及(ji)(ji)有(you)(you)效期(qi)1.本(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)體(ti)(ti)(ti)(ti)外定(ding)(ding)量(liang)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)血(xue)清、血(xue)漿、組織勻(yun)漿及(ji)(ji)相(xiang)關液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)Rubisco活(huo)(huo)化(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(mei)(RCA)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)量(liang)；2.試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)：2-8℃；3.有(you)(you)效期(qi)：6個月。植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)Rubisco活(huo)(huo)化(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(mei)(RCA)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集(ji)及(ji)(ji)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)1.血(xue)清：全血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)請(qing)(qing)(qing)(qing)(qing)于(yu)(yu)(yu)室溫(wen)放(fang)置(zhi)2小(xiao)時(shi)或(huo)(huo)(huo)(huo)(huo)(huo)4℃過(guo)(guo)一(yi)晚(wan)后(hou)(hou)(hou)于(yu)(yu)(yu)1000 x g離(li)心20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，取上(shang)(shang)清即可(ke)(ke)(ke)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)避(bi)(bi)免反(fan)(fan)(fan)復(fu)凍(dong)融(rong)(rong)。 2.血(xue)漿：可(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)(huo)(huo)肝(gan)素(su)(su)作(zuo)(zuo)為(wei)(wei)(wei)(wei)抗(kang)(kang)(kang)(kang)凝劑(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)采(cai)集(ji)后(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)于(yu)(yu)(yu)2 - 8° C 1000 x g離(li)心15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，或(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)避(bi)(bi)免反(fan)(fan)(fan)復(fu)凍(dong)融(rong)(rong)。 3.細(xi)胞培(pei)養(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)清或(huo)(huo)(huo)(huo)(huo)(huo)其它生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)：1000 x g離(li)心20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，取上(shang)(shang)清即可(ke)(ke)(ke)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)避(bi)(bi)免反(fan)(fan)(fan)復(fu)凍(dong)融(rong)(rong)。 (注(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)溶血(xue)會影(ying)響**檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)(jie)果(guo)(guo)(guo)，因(yin)此溶血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)不(bu)(bu)宜進(jin)(jin)行(xing)此項(xiang)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。) 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)Rubisco活(huo)(huo)化(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(mei)(RCA)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)   標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)(yuan)則(ze)：首先通過(guo)(guo)文獻檢(jian)(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)(fang)式(shi)了(le)(le)解(jie)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含(han)量(liang)，確(que)(que)定(ding)(ding)適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)。只有(you)(you)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范(fan)圍內(nei)(nei)(nei)，檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)(jie)(jie)果(guo)(guo)(guo)才(cai)是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)程中(zhong)(zhong)，應(ying)(ying)(ying)(ying)做(zuo)好詳細(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)錄。計(ji)(ji)(ji)算濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)時(shi)，稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)了(le)(le)“N”倍(bei)(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)應(ying)(ying)(ying)(ying)再乘(cheng)以(yi)(yi)(yi)(yi)“N”。    標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)(yuan)則(ze)：2瓶(ping)，每(mei)(mei)瓶(ping)臨用(yong)(yong)(yong)(yong)(yong)前以(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至1ml，蓋好后(hou)(hou)(hou)靜置(zhi)10分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)上(shang)(shang)，然后(hou)(hou)(hou)反(fan)(fan)(fan)復(fu)顛倒/搓(cuo)動(dong)以(yi)(yi)(yi)(yi)助(zhu)溶解(jie)，其濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)(wei)300 pg/ml，做(zuo)系列倍(bei)(bei)(bei)比(bi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)別稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)直(zhi)接作(zuo)(zuo)為(wei)(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)(yong)前15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。 如(ru)(ru)(ru)(ru)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取0.5ml（不(bu)(bu)要(yao)(yao)少(shao)于(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)含(han)0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中(zhong)(zhong)，混(hun)(hun)(hun)(hun)勻(yun)即可(ke)(ke)(ke)，其余(yu)(yu)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)以(yi)(yi)(yi)(yi)此類(lei)推(tui)。    生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)(yuan)則(ze)：臨用(yong)(yong)(yong)(yong)(yong)前以(yi)(yi)(yi)(yi)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前根(gen)(gen)(gen)(gen)(gen)據(ju)(ju)(ju)(ju)預(yu)先計(ji)(ji)(ji)算好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)次(ci)(ci)實(shi)(shi)(shi)(shi)驗所(suo)(suo)需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)(shi)際(ji)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)應(ying)(ying)(ying)(ying)多配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)(ru)10μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混(hun)(hun)(hun)(hun)勻(yun)，在使(shi)用(yong)(yong)(yong)(yong)(yong)前一(yi)小(xiao)時(shi)內(nei)(nei)(nei)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。    辣(la)(la)根(gen)(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)(yuan)則(ze)：臨用(yong)(yong)(yong)(yong)(yong)前以(yi)(yi)(yi)(yi)辣(la)(la)根(gen)(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前根(gen)(gen)(gen)(gen)(gen)據(ju)(ju)(ju)(ju)預(yu)先計(ji)(ji)(ji)算好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)次(ci)(ci)實(shi)(shi)(shi)(shi)驗所(suo)(suo)需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)(shi)際(ji)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)應(ying)(ying)(ying)(ying)多配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)(ru)10μl辣(la)(la)根(gen)(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)加(jia)(jia)(jia)(jia)(jia)(jia)990μl辣(la)(la)根(gen)(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混(hun)(hun)(hun)(hun)勻(yun)，在使(shi)用(yong)(yong)(yong)(yong)(yong)前一(yi)小(xiao)時(shi)內(nei)(nei)(nei)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)Rubisco活(huo)(huo)化(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(mei)(RCA)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)操作(zuo)(zuo)步驟   實(shi)(shi)(shi)(shi)驗開始前，請(qing)(qing)(qing)(qing)(qing)提(ti)前配(pei)(pei)置(zhi)好所(suo)(suo)有(you)(you)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)或(huo)(huo)(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)時(shi)，均需混(hun)(hun)(hun)(hun)勻(yun)，混(hun)(hun)(hun)(hun)勻(yun)時(shi)盡(jin)量(liang)避(bi)(bi)免起(qi)泡(pao)。每(mei)(mei)次(ci)(ci)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)該(gai)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)。如(ru)(ru)(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)過(guo)(guo)高時(shi)，用(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)進(jin)(jin)行(xing)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)使(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)圍。 1.加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)別設(she)空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)(kong)、待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)。空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)100μl，余(yu)(yu)孔(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)(huo)(huo)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)意不(bu)(bu)要(yao)(yao)有(you)(you)氣泡(pao)，加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)于(yu)(yu)(yu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)底(di)(di)(di)部，盡(jin)量(liang)不(bu)(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)壁(bi)，輕(qing)(qing)輕(qing)(qing)晃動(dong)混(hun)(hun)(hun)(hun)勻(yun)，酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)蓋或(huo)(huo)(huo)(huo)(huo)(huo)覆(fu)膜，37℃反(fan)(fan)(fan)應(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)(wei)保(bao)(bao)(bao)(bao)證(zheng)實(shi)(shi)(shi)(shi)驗結(jie)(jie)(jie)果(guo)(guo)(guo)有(you)(you)效性，每(mei)(mei)次(ci)(ci)實(shi)(shi)(shi)(shi)驗請(qing)(qing)(qing)(qing)(qing)使(shi)用(yong)(yong)(yong)(yong)(yong)新(xin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶液(ye)(ye)(ye)(ye)(ye)(ye)。 2.棄(qi)去(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)干，不(bu)(bu)用(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)滌(di)。每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混(hun)(hun)(hun)(hun)勻(yun)，在使(shi)用(yong)(yong)(yong)(yong)(yong)前一(yi)小(xiao)時(shi)內(nei)(nei)(nei)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 3.溫(wen)育60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)，棄(qi)去(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)干，洗(xi)(xi)板(ban)(ban)(ban)(ban)3次(ci)(ci)，每(mei)(mei)次(ci)(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)干。4.每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)辣(la)(la)根(gen)(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)（同生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。5.溫(wen)育60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)，棄(qi)去(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)干，洗(xi)(xi)板(ban)(ban)(ban)(ban)5次(ci)(ci)，每(mei)(mei)次(ci)(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)干。6.依(yi)(yi)(yi)序每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶液(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)(bi)光顯(xian)色（30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)，此時(shi)肉眼可(ke)(ke)(ke)見(jian)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前3-4孔(kong)(kong)(kong)(kong)(kong)(kong)有(you)(you)明顯(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)藍(lan)(lan)色，后(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)梯度(du)(du)(du)不(bu)(bu)明顯(xian)，即可(ke)(ke)(ke)終止）。7.依(yi)(yi)(yi)序每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)終止溶液(ye)(ye)(ye)(ye)(ye)(ye)50μl，終止反(fan)(fan)(fan)應(ying)(ying)(ying)(ying)（此時(shi)藍(lan)(lan)色立(li)轉(zhuan)黃(huang)色）。終止液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)順序應(ying)(ying)(ying)(ying)盡(jin)量(liang)與底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)順序相(xiang)同。為(wei)(wei)(wei)(wei)了(le)(le)保(bao)(bao)(bao)(bao)證(zheng)實(shi)(shi)(shi)(shi)驗結(jie)(jie)(jie)果(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)(que)性，底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)(fan)(fan)應(ying)(ying)(ying)(ying)時(shi)間到(dao)后(hou)(hou)(hou)應(ying)(ying)(ying)(ying)盡(jin)快加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)終止液(ye)(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)儀在450nm波長(chang)依(yi)(yi)(yi)序測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光密度(du)(du)(du)（OD值(zhi)(zhi)(zhi)）。 在加(jia)(jia)(jia)(jia)(jia)(jia)終止液(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)內(nei)(nei)(nei)進(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)Rubisco活(huo)(huo)化(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(mei)(RCA)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)注(zhu)意事項(xiang)1.用(yong)(yong)(yong)(yong)(yong)戶在初次(ci)(ci)使(shi)用(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)時(shi)，應(ying)(ying)(ying)(ying)將(jiang)各(ge)種試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)管離(li)心數(shu)(shu)分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)集(ji)中(zhong)(zhong)到(dao)管底(di)(di)(di)。2.每(mei)(mei)次(ci)(ci)實(shi)(shi)(shi)(shi)驗留一(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)為(wei)(wei)(wei)(wei)空(kong)白(bai)調零孔(kong)(kong)(kong)(kong)(kong)(kong)，該(gai)孔(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)任何試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)，只是**加(jia)(jia)(jia)(jia)(jia)(jia)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶液(ye)(ye)(ye)(ye)(ye)(ye)及(ji)(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)時(shi)先用(yong)(yong)(yong)(yong)(yong)此孔(kong)(kong)(kong)(kong)(kong)(kong)調OD值(zhi)(zhi)(zhi)至零。 3.為(wei)(wei)(wei)(wei)防止樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)(shi)(shi)(shi)驗時(shi)將(jiang)反(fan)(fan)(fan)應(ying)(ying)(ying)(ying)板(ban)(ban)(ban)(ban)放(fang)于(yu)(yu)(yu)鋪(pu)有(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)(he)(he)(he)內(nei)(nei)(nei)，酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)蓋或(huo)(huo)(huo)(huo)(huo)(huo)覆(fu)膜。 4.未使(shi)用(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)或(huo)(huo)(huo)(huo)(huo)(huo)者試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)，請(qing)(qing)(qing)(qing)(qing)于(yu)(yu)(yu)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)(la)根(gen)(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)請(qing)(qing)(qing)(qing)(qing)依(yi)(yi)(yi)據(ju)(ju)(ju)(ju)所(suo)(suo)需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)配(pei)(pei)置(zhi)使(shi)用(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)(qing)(qing)(qing)勿重復(fu)使(shi)用(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)(huo)(huo)、辣(la)(la)根(gen)(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)。 5.建議(yi)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)均設(she)雙(shuang)孔(kong)(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)，以(yi)(yi)(yi)(yi)保(bao)(bao)(bao)(bao)證(zheng)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)(jie)果(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)(que)性。 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)Rubisco活(huo)(huo)化(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(mei)(RCA)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)洗(xi)(xi)板(ban)(ban)(ban)(ban)方(fang)(fang)(fang)法手工(gong)(gong)(gong)洗(xi)(xi)板(ban)(ban)(ban)(ban)方(fang)(fang)(fang)法：吸(xi)去(qu)（不(bu)(bu)可(ke)(ke)(ke)觸及(ji)(ji)板(ban)(ban)(ban)(ban)壁(bi)）或(huo)(huo)(huo)(huo)(huo)(huo)甩(shuai)掉酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)內(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)；在實(shi)(shi)(shi)(shi)驗臺(tai)上(shang)(shang)鋪(pu)墊幾(ji)層(ceng)吸(xi)水紙(zhi)，酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)朝下(xia)用(yong)(yong)(yong)(yong)(yong)力拍幾(ji)次(ci)(ci)；將(jiang)推(tui)薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)緩(huan)沖液(ye)(ye)(ye)(ye)(ye)(ye)至少(shao)0.3ml注(zhu)入(ru)(ru)孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)，浸泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。根(gen)(gen)(gen)(gen)(gen)據(ju)(ju)(ju)(ju)需要(yao)(yao)，重復(fu)此過(guo)(guo)程數(shu)(shu)次(ci)(ci)。 自動(dong)洗(xi)(xi)板(ban)(ban)(ban)(ban)：如(ru)(ru)(ru)(ru)果(guo)(guo)(guo)有(you)(you)自動(dong)洗(xi)(xi)板(ban)(ban)(ban)(ban)機，應(ying)(ying)(ying)(ying)在熟練使(shi)用(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)再用(yong)(yong)(yong)(yong)(yong)到(dao)正(zheng)式(shi)實(shi)(shi)(shi)(shi)驗過(guo)(guo)程中(zhong)(zhong)。 計(ji)(ji)(ji)算   以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在半對數(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)繪(hui)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)，根(gen)(gen)(gen)(gen)(gen)據(ju)(ju)(ju)(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)查出(chu)(chu)相(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)；再乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)；或(huo)(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)與OD值(zhi)(zhi)(zhi)計(ji)(ji)(ji)算出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)(xian)(xian)回(hui)歸方(fang)(fang)(fang)程式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代(dai)入(ru)(ru)方(fang)(fang)(fang)程式(shi)，計(ji)(ji)(ji)算出(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)，再乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)，即為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)(shi)際(ji)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)。 注(zhu)意事項(xiang)1.當混(hun)(hun)(hun)(hun)合蛋白(bai)溶液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)應(ying)(ying)(ying)(ying)盡(jin)量(liang)輕(qing)(qing)緩(huan)，避(bi)(bi)免起(qi)泡(pao)。2.洗(xi)(xi)滌(di)過(guo)(guo)程非常重要(yao)(yao)，不(bu)(bu)充分(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)易(yi)造成假陽性。3.一(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)時(shi)間**控制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)在5分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)，如(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)數(shu)(shu)量(liang)多，推(tui)薦使(shi)用(yong)(yong)(yong)(yong)(yong)排(pai)槍加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)。4.請(qing)(qing)(qing)(qing)(qing)每(mei)(mei)次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)，做(zuo)復(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)。5.如(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)含(han)量(liang)過(guo)(guo)高，請(qing)(qing)(qing)(qing)(qing)先稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)再測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)，計(ji)(ji)(ji)算時(shi)請(qing)(qing)(qing)(qing)(qing)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)。6.在配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)溶液(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)，請(qing)(qing)(qing)(qing)(qing)以(yi)(yi)(yi)(yi)相(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，不(bu)(bu)能混(hun)(hun)(hun)(hun)淆。7.底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)(qing)(qing)(qing)避(bi)(bi)光保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)。 8.不(bu)(bu)要(yao)(yao)用(yong)(yong)(yong)(yong)(yong)其它生(sheng)(sheng)(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)替換試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)。 注(zhu)意事項(xiang)：收(shou)集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)前必(bi)須清楚要(yao)(yao)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成份(fen)是否足夠穩定(ding)(ding)。對收(shou)集(ji)后(hou)(hou)(hou)當天進(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)，儲存(cun)(cun)(cun)(cun)(cun)在4 ℃備用(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)(ru)(ru)有(you)(you)特殊原(yuan)(yuan)(yuan)因(yin)需要(yao)(yao)周期(qi)收(shou)集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)， 將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)及(ji)(ji)時(shi)分(fen)(fen)(fen)(fen)裝后(hou)(hou)(hou)放(fang)在-20 ℃或(huo)(huo)(huo)(huo)(huo)(huo)-70 ℃條(tiao)件(jian)(jian)下(xia)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)。避(bi)(bi)免反(fan)(fan)(fan)復(fu)凍(dong)融(rong)(rong)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)48 小(xiao)時(shi)，-20 ℃可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)1 個月。-70度(du)(du)(du)可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)6 個月。部分(fen)(fen)(fen)(fen)激素(su)(su)類(lei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)需添加(jia)(jia)(jia)(jia)(jia)(jia)抑(yi)肽(tai)酶(mei)(mei)(mei)(mei)(mei)(mei)。計(ji)(ji)(ji)算：以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)繪(hui)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)，根(gen)(gen)(gen)(gen)(gen)據(ju)(ju)(ju)(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)查出(chu)(chu)相(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)；再乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)；或(huo)(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)與OD值(zhi)(zhi)(zhi)計(ji)(ji)(ji)算出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)(xian)(xian)回(hui)歸方(fang)(fang)(fang)程式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代(dai)入(ru)(ru)方(fang)(fang)(fang)程式(shi)，計(ji)(ji)(ji)算出(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)，再乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)，即為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)(shi)際(ji)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)。 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)Rubisco活(huo)(huo)化(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(mei)(RCA)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)性能1.樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)(xian)性回(hui)歸與預(yu)期(qi)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)相(xiang)關系數(shu)(shu)R值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)0.92以(yi)(yi)(yi)(yi)上(shang)(shang)。2.批內(nei)(nei)(nei)與批見(jian)應(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)別小(xiao)于(yu)(yu)(yu)9%和(he)(he)15% 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)Rubisco活(huo)(huo)化(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(mei)(RCA)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)

實(shi)驗原(yuan)理   試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)采用(yong)(yong)(yong)(yong)(yong)雙(shuang)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)一(yi)步夾心(xin)法酶(mei)(mei)聯免(mian)疫吸附試(shi)(shi)(shi)(shi)(shi)(shi)驗（ELISA）。往預先(xian)(xian)包(bao)被(bei)丙(bing)二(er)(er)(er)醛(quan)(quan)(quan)(quan)(MDA)捕獲抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)包(bao)被(bei)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，依(yi)(yi)次(ci)(ci)(ci)加(jia)(jia)(jia)(jia)入(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)，經(jing)過(guo)(guo)(guo)(guo)(guo)溫育(yu)并徹底洗(xi)(xi)(xi)滌。用(yong)(yong)(yong)(yong)(yong)底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯色(se)(se)(se)，TMB在(zai)(zai)過(guo)(guo)(guo)(guo)(guo)氧化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)催化下(xia)轉(zhuan)化成(cheng)藍色(se)(se)(se)，并在(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)下(xia)轉(zhuan)化成(cheng)＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)色(se)(se)(se)。顏色(se)(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)丙(bing)二(er)(er)(er)醛(quan)(quan)(quan)(quan)(MDA)呈(cheng)正相關(guan)。用(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)450nm 波(bo)(bo)長下(xia)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)吸光(guang)(guang)(guang)度(du)(du)(du)(du)(du)（OD 值(zhi)(zhi)(zhi)(zhi)），計(ji)(ji)算樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)度(du)(du)(du)(du)(du)。保(bao)(bao)存(cun)條(tiao)件(jian)及(ji)(ji)(ji)(ji)有(you)(you)(you)(you)(you)效期1.本(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)體(ti)(ti)(ti)外定(ding)(ding)(ding)量(liang)(liang)(liang)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)血(xue)清(qing)、血(xue)漿、組織勻(yun)漿及(ji)(ji)(ji)(ji)相關(guan)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)中(zhong)丙(bing)二(er)(er)(er)醛(quan)(quan)(quan)(quan)(MDA)的(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)(liang)；2.試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)保(bao)(bao)存(cun)：2-8℃；3.有(you)(you)(you)(you)(you)效期：6個(ge)(ge)月(yue)。植物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)丙(bing)二(er)(er)(er)醛(quan)(quan)(quan)(quan)(MDA)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)采集(ji)(ji)及(ji)(ji)(ji)(ji)保(bao)(bao)存(cun)1.血(xue)清(qing)：全血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)請(qing)(qing)(qing)于(yu)(yu)室溫放(fang)置2小(xiao)時(shi)(shi)(shi)或(huo)(huo)(huo)4℃過(guo)(guo)(guo)(guo)(guo)一(yi)晚后(hou)(hou)(hou)(hou)(hou)于(yu)(yu)1000 x g離心(xin)20分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，取(qu)(qu)上(shang)(shang)(shang)清(qing)即(ji)可(ke)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放(fang)于(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)免(mian)反復凍融。 2.血(xue)漿：可(ke)用(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)肝素(su)(su)(su)(su)(su)(su)(su)作(zuo)(zuo)(zuo)(zuo)為(wei)抗(kang)(kang)(kang)(kang)凝劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)采集(ji)(ji)后(hou)(hou)(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)于(yu)(yu)2 - 8° C 1000 x g離心(xin)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，或(huo)(huo)(huo)將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放(fang)于(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)免(mian)反復凍融。 3.細胞培養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)清(qing)或(huo)(huo)(huo)其(qi)(qi)(qi)它(ta)(ta)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)：1000 x g離心(xin)20分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，取(qu)(qu)上(shang)(shang)(shang)清(qing)即(ji)可(ke)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放(fang)于(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)免(mian)反復凍融。 (注：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)溶(rong)(rong)血(xue)會影響(xiang)**檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果，因此(ci)(ci)溶(rong)(rong)血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)不(bu)(bu)(bu)(bu)宜進(jin)行(xing)此(ci)(ci)項(xiang)(xiang)(xiang)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)。) 植物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)丙(bing)二(er)(er)(er)醛(quan)(quan)(quan)(quan)(MDA)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)   標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：首先(xian)(xian)通(tong)過(guo)(guo)(guo)(guo)(guo)文獻檢(jian)(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)式(shi)(shi)了(le)解待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)大致(zhi)含量(liang)(liang)(liang)，確(que)定(ding)(ding)(ding)適當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)。只(zhi)(zhi)有(you)(you)(you)(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍內(nei)(nei)(nei)，檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果才是(shi)(shi)準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)(guo)程(cheng)中(zhong)，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)做(zuo)好(hao)(hao)詳細的(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)錄。計(ji)(ji)算濃(nong)度(du)(du)(du)(du)(du)時(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)了(le)“N”倍(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)(du)(du)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)再(zai)乘以(yi)(yi)“N”。    標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：2瓶，每(mei)(mei)(mei)瓶臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)1ml，蓋好(hao)(hao)后(hou)(hou)(hou)(hou)(hou)靜置10分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)以(yi)(yi)上(shang)(shang)(shang)，然后(hou)(hou)(hou)(hou)(hou)反復顛倒/搓動(dong)以(yi)(yi)助溶(rong)(rong)解，其(qi)(qi)(qi)濃(nong)度(du)(du)(du)(du)(du)為(wei)300 pg/ml，做(zuo)系(xi)列倍(bei)比(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)(fen)別稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)直接(jie)作(zuo)(zuo)(zuo)(zuo)為(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)度(du)(du)(du)(du)(du)0 pg/ml，臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。 如配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)：取(qu)(qu)0.5ml（不(bu)(bu)(bu)(bu)要少于(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)入(ru)含0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中(zhong)，混(hun)(hun)勻(yun)即(ji)可(ke)，其(qi)(qi)(qi)余濃(nong)度(du)(du)(du)(du)(du)以(yi)(yi)此(ci)(ci)類推。    生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)據(ju)(ju)預先(xian)(xian)計(ji)(ji)算好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)(ci)實(shi)驗所需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際(ji)(ji)(ji)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)多(duo)(duo)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如10μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)加(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(li)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)勻(yun)，在(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)小(xiao)時(shi)(shi)(shi)內(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。    辣(la)(la)根(gen)過(guo)(guo)(guo)(guo)(guo)氧化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)(su)(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)辣(la)(la)根(gen)過(guo)(guo)(guo)(guo)(guo)氧化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)據(ju)(ju)預先(xian)(xian)計(ji)(ji)算好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)(ci)實(shi)驗所需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際(ji)(ji)(ji)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)多(duo)(duo)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如10μl辣(la)(la)根(gen)過(guo)(guo)(guo)(guo)(guo)氧化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)(su)(su)(su)(su)(su)加(jia)(jia)(jia)(jia)990μl辣(la)(la)根(gen)過(guo)(guo)(guo)(guo)(guo)氧化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(li)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)勻(yun)，在(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)小(xiao)時(shi)(shi)(shi)內(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。 植物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)丙(bing)二(er)(er)(er)醛(quan)(quan)(quan)(quan)(MDA)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)操(cao)作(zuo)(zuo)(zuo)(zuo)步驟   實(shi)驗開始前(qian)(qian)(qian)，請(qing)(qing)(qing)提前(qian)(qian)(qian)配(pei)(pei)(pei)(pei)置好(hao)(hao)所有(you)(you)(you)(you)(you)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)或(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)，均(jun)需(xu)(xu)混(hun)(hun)勻(yun)，混(hun)(hun)勻(yun)時(shi)(shi)(shi)盡(jin)(jin)量(liang)(liang)(liang)避(bi)免(mian)起泡(pao)。每(mei)(mei)(mei)次(ci)(ci)(ci)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)(ying)(ying)(ying)該(gai)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)。如樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)度(du)(du)(du)(du)(du)過(guo)(guo)(guo)(guo)(guo)高時(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)進(jin)行(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)使(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)符合(he)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)范圍。 1.加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)別設空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)、待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)別加(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)100μl，注意(yi)(yi)不(bu)(bu)(bu)(bu)要有(you)(you)(you)(you)(you)氣泡(pao)，加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)將樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)于(yu)(yu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部(bu)，盡(jin)(jin)量(liang)(liang)(liang)不(bu)(bu)(bu)(bu)觸及(ji)(ji)(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)(qing)(qing)輕(qing)(qing)(qing)晃動(dong)混(hun)(hun)勻(yun)，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)(jia)(jia)上(shang)(shang)(shang)蓋或(huo)(huo)(huo)覆膜，37℃反應(ying)(ying)(ying)(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。 為(wei)保(bao)(bao)證實(shi)驗結(jie)果有(you)(you)(you)(you)(you)效性，每(mei)(mei)(mei)次(ci)(ci)(ci)實(shi)驗請(qing)(qing)(qing)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 2.棄(qi)(qi)去(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)(shuai)干(gan)，不(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)(xi)滌。每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取(qu)(qu)1μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)加(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(li)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)勻(yun)，在(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)小(xiao)時(shi)(shi)(shi)內(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。 3.溫育(yu)60分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)(hou)(hou)(hou)，棄(qi)(qi)去(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)(shuai)干(gan)，洗(xi)(xi)(xi)板(ban)(ban)(ban)3次(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)干(gan)。4.每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)辣(la)(la)根(gen)過(guo)(guo)(guo)(guo)(guo)氧化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)(su)(su)(su)(su)(su)工(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。5.溫育(yu)60分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)(hou)(hou)(hou)，棄(qi)(qi)去(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)(shuai)干(gan)，洗(xi)(xi)(xi)板(ban)(ban)(ban)5次(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)干(gan)。6.依(yi)(yi)序(xu)每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)光(guang)(guang)(guang)顯色(se)(se)(se)（30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)，此(ci)(ci)時(shi)(shi)(shi)肉(rou)眼可(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)有(you)(you)(you)(you)(you)明(ming)顯的(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)(du)(du)藍色(se)(se)(se)，后(hou)(hou)(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)梯度(du)(du)(du)(du)(du)不(bu)(bu)(bu)(bu)明(ming)顯，即(ji)可(ke)終(zhong)止(zhi)）。7.依(yi)(yi)序(xu)每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)終(zhong)止(zhi)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止(zhi)反應(ying)(ying)(ying)(ying)(ying)(ying)(ying)（此(ci)(ci)時(shi)(shi)(shi)藍色(se)(se)(se)立轉(zhuan)黃(huang)色(se)(se)(se)）。終(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)入(ru)順序(xu)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)(jin)量(liang)(liang)(liang)與底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)入(ru)順序(xu)相同。為(wei)了(le)保(bao)(bao)證實(shi)驗結(jie)果的(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性，底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反應(ying)(ying)(ying)(ying)(ying)(ying)(ying)時(shi)(shi)(shi)間到后(hou)(hou)(hou)(hou)(hou)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)(jin)快加(jia)(jia)(jia)(jia)入(ru)終(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)聯儀在(zai)(zai)450nm波(bo)(bo)長依(yi)(yi)序(xu)測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)(guang)(guang)密度(du)(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)）。 在(zai)(zai)加(jia)(jia)(jia)(jia)終(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)以(yi)(yi)內(nei)(nei)(nei)進(jin)行(xing)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)。植物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)丙(bing)二(er)(er)(er)醛(quan)(quan)(quan)(quan)(MDA)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)注意(yi)(yi)事(shi)(shi)項(xiang)(xiang)(xiang)1.用(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)初次(ci)(ci)(ci)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)時(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)將各(ge)種試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)管離心(xin)數(shu)(shu)(shu)(shu)分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，以(yi)(yi)便試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)集(ji)(ji)中(zhong)到管底。2.每(mei)(mei)(mei)次(ci)(ci)(ci)實(shi)驗留一(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)(zuo)(zuo)為(wei)空白(bai)調零孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，該(gai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)(bu)加(jia)(jia)(jia)(jia)任何試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)，只(zhi)(zhi)是(shi)(shi)**加(jia)(jia)(jia)(jia)底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)及(ji)(ji)(ji)(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)時(shi)(shi)(shi)先(xian)(xian)用(yong)(yong)(yong)(yong)(yong)此(ci)(ci)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)調OD值(zhi)(zhi)(zhi)(zhi)至(zhi)零。 3.為(wei)防(fang)止(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)(shi)(shi)(shi)驗時(shi)(shi)(shi)將反應(ying)(ying)(ying)(ying)(ying)(ying)(ying)板(ban)(ban)(ban)放(fang)于(yu)(yu)鋪(pu)有(you)(you)(you)(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)內(nei)(nei)(nei)，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)(jia)(jia)上(shang)(shang)(shang)蓋或(huo)(huo)(huo)覆膜。 4.未使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)或(huo)(huo)(huo)者試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)，請(qing)(qing)(qing)于(yu)(yu)2-8℃保(bao)(bao)存(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)(la)根(gen)過(guo)(guo)(guo)(guo)(guo)氧化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)(su)(su)(su)(su)(su)工(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)請(qing)(qing)(qing)依(yi)(yi)據(ju)(ju)所需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)置使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)(qing)勿(wu)重復使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)、辣(la)(la)根(gen)過(guo)(guo)(guo)(guo)(guo)氧化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)(su)(su)(su)(su)(su)工(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5.建議檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)均(jun)設雙(shuang)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，以(yi)(yi)保(bao)(bao)證檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果的(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性。 植物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)丙(bing)二(er)(er)(er)醛(quan)(quan)(quan)(quan)(MDA)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)洗(xi)(xi)(xi)板(ban)(ban)(ban)方(fang)(fang)法手工(gong)洗(xi)(xi)(xi)板(ban)(ban)(ban)方(fang)(fang)法：吸去(qu)（不(bu)(bu)(bu)(bu)可(ke)觸及(ji)(ji)(ji)(ji)板(ban)(ban)(ban)壁）或(huo)(huo)(huo)甩(shuai)(shuai)掉酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)內(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)；在(zai)(zai)實(shi)驗臺上(shang)(shang)(shang)鋪(pu)墊(dian)幾(ji)層吸水紙，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)朝(chao)下(xia)用(yong)(yong)(yong)(yong)(yong)力拍幾(ji)次(ci)(ci)(ci)；將推薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)(xi)滌緩(huan)(huan)沖液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)至(zhi)少0.3ml注入(ru)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)，浸泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。根(gen)據(ju)(ju)需(xu)(xu)要，重復此(ci)(ci)過(guo)(guo)(guo)(guo)(guo)程(cheng)數(shu)(shu)(shu)(shu)次(ci)(ci)(ci)。 自動(dong)洗(xi)(xi)(xi)板(ban)(ban)(ban)：如果有(you)(you)(you)(you)(you)自動(dong)洗(xi)(xi)(xi)板(ban)(ban)(ban)機(ji)，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)在(zai)(zai)熟練使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)(hou)(hou)再(zai)用(yong)(yong)(yong)(yong)(yong)到正式(shi)(shi)實(shi)驗過(guo)(guo)(guo)(guo)(guo)程(cheng)中(zhong)。 計(ji)(ji)算   以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)(du)(du)為(wei)橫(heng)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)(shu)(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)(zhi)為(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通(tong)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)半對數(shu)(shu)(shu)(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)繪(hui)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)，根(gen)據(ju)(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)由(you)(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)查出(chu)(chu)(chu)相應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)(du)(du)；再(zai)乘以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)(du)(du)與OD值(zhi)(zhi)(zhi)(zhi)計(ji)(ji)算出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)回(hui)歸(gui)方(fang)(fang)程(cheng)式(shi)(shi)，將樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)代入(ru)方(fang)(fang)程(cheng)式(shi)(shi)，計(ji)(ji)算出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)度(du)(du)(du)(du)(du)，再(zai)乘以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)，即(ji)為(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際(ji)(ji)(ji)濃(nong)度(du)(du)(du)(du)(du)。 注意(yi)(yi)事(shi)(shi)項(xiang)(xiang)(xiang)1.當(dang)混(hun)(hun)合(he)蛋白(bai)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)(jin)量(liang)(liang)(liang)輕(qing)(qing)(qing)緩(huan)(huan)，避(bi)免(mian)起泡(pao)。2.洗(xi)(xi)(xi)滌過(guo)(guo)(guo)(guo)(guo)程(cheng)非(fei)常重要，不(bu)(bu)(bu)(bu)充分(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)(xi)滌易造成(cheng)假陽性。3.一(yi)次(ci)(ci)(ci)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)間**控制(zhi)(zhi)(zhi)(zhi)(zhi)在(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)，如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)數(shu)(shu)(shu)(shu)量(liang)(liang)(liang)多(duo)(duo)，推薦使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)。4.請(qing)(qing)(qing)每(mei)(mei)(mei)次(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)，做(zuo)復孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。5.如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)中(zhong)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)含量(liang)(liang)(liang)過(guo)(guo)(guo)(guo)(guo)高，請(qing)(qing)(qing)先(xian)(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)再(zai)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，計(ji)(ji)算時(shi)(shi)(shi)請(qing)(qing)(qing)乘以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)。6.在(zai)(zai)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)工(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)，請(qing)(qing)(qing)以(yi)(yi)相應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，不(bu)(bu)(bu)(bu)能(neng)(neng)混(hun)(hun)淆。7.底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)(qing)避(bi)光(guang)(guang)(guang)保(bao)(bao)存(cun)。 8.不(bu)(bu)(bu)(bu)要用(yong)(yong)(yong)(yong)(yong)其(qi)(qi)(qi)它(ta)(ta)生(sheng)(sheng)產廠(chang)家的(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)替換(huan)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)。 注意(yi)(yi)事(shi)(shi)項(xiang)(xiang)(xiang)：收(shou)(shou)集(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)前(qian)(qian)(qian)必須清(qing)楚要檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)份是(shi)(shi)否足(zu)夠穩定(ding)(ding)(ding)。對收(shou)(shou)集(ji)(ji)后(hou)(hou)(hou)(hou)(hou)當(dang)天進(jin)行(xing)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)，儲存(cun)在(zai)(zai)4 ℃備(bei)用(yong)(yong)(yong)(yong)(yong)，如有(you)(you)(you)(you)(you)特殊原(yuan)因需(xu)(xu)要周期收(shou)(shou)集(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)， 將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)及(ji)(ji)(ji)(ji)時(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)裝后(hou)(hou)(hou)(hou)(hou)放(fang)在(zai)(zai)-20 ℃或(huo)(huo)(huo)-70 ℃條(tiao)件(jian)下(xia)保(bao)(bao)存(cun)。避(bi)免(mian)反復凍融。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)2 -8 ℃可(ke)保(bao)(bao)存(cun)48 小(xiao)時(shi)(shi)(shi)，-20 ℃可(ke)保(bao)(bao)存(cun)1 個(ge)(ge)月(yue)。-70度(du)(du)(du)(du)(du)可(ke)保(bao)(bao)存(cun)6 個(ge)(ge)月(yue)。部(bu)分(fen)(fen)(fen)(fen)(fen)激(ji)素(su)(su)(su)(su)(su)(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)需(xu)(xu)添加(jia)(jia)(jia)(jia)抑(yi)肽酶(mei)(mei)。計(ji)(ji)算：以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)(du)(du)為(wei)橫(heng)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)為(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)繪(hui)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)，根(gen)據(ju)(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)由(you)(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)查出(chu)(chu)(chu)相應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)(du)(du)；再(zai)乘以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)(du)(du)與OD值(zhi)(zhi)(zhi)(zhi)計(ji)(ji)算出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)回(hui)歸(gui)方(fang)(fang)程(cheng)式(shi)(shi)，將樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)代入(ru)方(fang)(fang)程(cheng)式(shi)(shi)，計(ji)(ji)算出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)度(du)(du)(du)(du)(du)，再(zai)乘以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)，即(ji)為(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際(ji)(ji)(ji)濃(nong)度(du)(du)(du)(du)(du)。 植物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)丙(bing)二(er)(er)(er)醛(quan)(quan)(quan)(quan)(MDA)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)性能(neng)(neng)1.樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)線(xian)性回(hui)歸(gui)與預期濃(nong)度(du)(du)(du)(du)(du)相關(guan)系(xi)數(shu)(shu)(shu)(shu)R值(zhi)(zhi)(zhi)(zhi)為(wei)0.92以(yi)(yi)上(shang)(shang)(shang)。2.批內(nei)(nei)(nei)與批見應(ying)(ying)(ying)(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)(fen)別小(xiao)于(yu)(yu)9%和(he)(he)(he)15% 植物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)丙(bing)二(er)(er)(er)醛(quan)(quan)(quan)(quan)(MDA)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)

實(shi)(shi)驗(yan)(yan)(yan)(yan)原理(li)   試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)采(cai)(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)一(yi)(yi)(yi)步夾心(xin)法(fa)酶(mei)(mei)(mei)(mei)(mei)聯免疫吸(xi)附(fu)試(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)（ELISA）。往預(yu)先包被(bei)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)氫酶(mei)(mei)(mei)(mei)(mei)(CAT)捕獲抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被(bei)微孔(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，依次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)，經過(guo)(guo)(guo)溫(wen)(wen)育(yu)并徹底(di)洗(xi)(xi)(xi)滌(di)(di)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯色，TMB在(zai)(zai)(zai)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)(hua)(hua)(hua)(hua)(hua)下(xia)轉化(hua)(hua)(hua)(hua)(hua)(hua)(hua)成藍(lan)色，并在(zai)(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作用(yong)(yong)(yong)(yong)(yong)(yong)(yong)下(xia)轉化(hua)(hua)(hua)(hua)(hua)(hua)(hua)成＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)(huang)色。顏(yan)色的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深(shen)淺和(he)(he)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)氫酶(mei)(mei)(mei)(mei)(mei)(CAT)呈正相(xiang)關(guan)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)(zai)450nm 波長下(xia)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)吸(xi)光(guang)度(du)(du)(du)(du)(du)(du)(du)（OD 值(zhi)），計(ji)(ji)算(suan)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。保(bao)(bao)(bao)存(cun)(cun)(cun)條件及(ji)(ji)有(you)效期(qi)1.本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于體(ti)(ti)(ti)(ti)外定(ding)(ding)(ding)量(liang)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)血(xue)(xue)清(qing)(qing)、血(xue)(xue)漿、組織(zhi)勻(yun)(yun)(yun)漿及(ji)(ji)相(xiang)關(guan)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)中(zhong)(zhong)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)氫酶(mei)(mei)(mei)(mei)(mei)(CAT)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)量(liang)；2.試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)保(bao)(bao)(bao)存(cun)(cun)(cun)：2-8℃；3.有(you)效期(qi)：6個(ge)月(yue)(yue)。植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)氫酶(mei)(mei)(mei)(mei)(mei)(CAT)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)(cai)集(ji)(ji)及(ji)(ji)保(bao)(bao)(bao)存(cun)(cun)(cun)1.血(xue)(xue)清(qing)(qing)：全血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)請于室溫(wen)(wen)放(fang)(fang)置2小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)4℃過(guo)(guo)(guo)一(yi)(yi)(yi)晚(wan)后(hou)(hou)(hou)于1000 x g離(li)心(xin)20分(fen)(fen)鐘(zhong)(zhong)(zhong)，取上(shang)清(qing)(qing)即(ji)可(ke)(ke)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)(fang)于-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)存(cun)(cun)(cun)，但(dan)(dan)應(ying)(ying)(ying)(ying)避(bi)(bi)免反(fan)(fan)復(fu)凍(dong)融。 2.血(xue)(xue)漿：可(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)肝素(su)(su)(su)作為(wei)(wei)抗(kang)(kang)(kang)(kang)凝劑(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)采(cai)(cai)集(ji)(ji)后(hou)(hou)(hou)30分(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)于2 - 8° C 1000 x g離(li)心(xin)15分(fen)(fen)鐘(zhong)(zhong)(zhong)，或(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)(fang)于-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)存(cun)(cun)(cun)，但(dan)(dan)應(ying)(ying)(ying)(ying)避(bi)(bi)免反(fan)(fan)復(fu)凍(dong)融。 3.細胞(bao)培養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)清(qing)(qing)或(huo)(huo)(huo)其(qi)它(ta)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)：1000 x g離(li)心(xin)20分(fen)(fen)鐘(zhong)(zhong)(zhong)，取上(shang)清(qing)(qing)即(ji)可(ke)(ke)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)(fang)于-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)存(cun)(cun)(cun)，但(dan)(dan)應(ying)(ying)(ying)(ying)避(bi)(bi)免反(fan)(fan)復(fu)凍(dong)融。 (注(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)溶血(xue)(xue)會(hui)影響**檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)果(guo)(guo)，因此(ci)溶血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)不(bu)(bu)(bu)(bu)宜進(jin)(jin)(jin)(jin)行(xing)(xing)此(ci)項檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)。) 植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)氫酶(mei)(mei)(mei)(mei)(mei)(CAT)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)   標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則(ze)：首先通(tong)過(guo)(guo)(guo)文獻(xian)檢(jian)(jian)(jian)索(suo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)式(shi)了解(jie)待測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含(han)量(liang)，確定(ding)(ding)(ding)適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)。只有(you)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范(fan)圍內(nei)，檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)(jie)果(guo)(guo)才是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)，應(ying)(ying)(ying)(ying)做好(hao)詳細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)錄。計(ji)(ji)算(suan)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)了“N”倍，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)應(ying)(ying)(ying)(ying)再(zai)乘(cheng)以(yi)(yi)(yi)(yi)“N”。    標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則(ze)：2瓶(ping)，每(mei)瓶(ping)臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至1ml，蓋(gai)好(hao)后(hou)(hou)(hou)靜置10分(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)上(shang)，然后(hou)(hou)(hou)反(fan)(fan)復(fu)顛倒/搓動以(yi)(yi)(yi)(yi)助(zhu)溶解(jie)，其(qi)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)300 pg/ml，做系(xi)列倍比(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)，分(fen)(fen)別稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)直接作為(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)15分(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 如(ru)(ru)(ru)(ru)(ru)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)：取0.5ml（不(bu)(bu)(bu)(bu)要少(shao)于0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)含(han)0.5ml樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中(zhong)(zhong)，混(hun)勻(yun)(yun)(yun)即(ji)可(ke)(ke)，其(qi)余濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)以(yi)(yi)(yi)(yi)此(ci)類(lei)推(tui)。    生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則(ze)：臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以(yi)(yi)(yi)(yi)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)根(gen)(gen)(gen)(gen)據(ju)預(yu)先計(ji)(ji)算(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)次(ci)(ci)實(shi)(shi)驗(yan)(yan)(yan)(yan)所需(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)際(ji)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)多配(pei)(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)(ru)(ru)10μl生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)勻(yun)(yun)(yun)，在(zai)(zai)(zai)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一(yi)(yi)(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)。    辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則(ze)：臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以(yi)(yi)(yi)(yi)辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)根(gen)(gen)(gen)(gen)據(ju)預(yu)先計(ji)(ji)算(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)次(ci)(ci)實(shi)(shi)驗(yan)(yan)(yan)(yan)所需(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)際(ji)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)多配(pei)(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)(ru)(ru)10μl辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)(su)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)勻(yun)(yun)(yun)，在(zai)(zai)(zai)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一(yi)(yi)(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)氫酶(mei)(mei)(mei)(mei)(mei)(CAT)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)操作步驟(zou)   實(shi)(shi)驗(yan)(yan)(yan)(yan)開始前(qian)(qian)(qian)(qian)，請提(ti)前(qian)(qian)(qian)(qian)配(pei)(pei)(pei)置好(hao)所有(you)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)或(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)，均(jun)需(xu)(xu)(xu)混(hun)勻(yun)(yun)(yun)，混(hun)勻(yun)(yun)(yun)時(shi)(shi)(shi)(shi)(shi)(shi)盡(jin)量(liang)避(bi)(bi)免起泡。每(mei)次(ci)(ci)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)該做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)。如(ru)(ru)(ru)(ru)(ru)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)過(guo)(guo)(guo)高時(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)進(jin)(jin)(jin)(jin)行(xing)(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)使(shi)(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)符合試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)范(fan)圍。 1.加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)：分(fen)(fen)別設(she)空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)、待測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)。空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)分(fen)(fen)別加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)待測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)意(yi)不(bu)(bu)(bu)(bu)要有(you)氣(qi)泡，加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)于酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)底(di)部(bu)，盡(jin)量(liang)不(bu)(bu)(bu)(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)壁(bi)(bi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)晃動混(hun)勻(yun)(yun)(yun)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)蓋(gai)或(huo)(huo)(huo)覆膜(mo)，37℃反(fan)(fan)應(ying)(ying)(ying)(ying)120分(fen)(fen)鐘(zhong)(zhong)(zhong)。 為(wei)(wei)保(bao)(bao)(bao)證實(shi)(shi)驗(yan)(yan)(yan)(yan)結(jie)(jie)果(guo)(guo)有(you)效性(xing)(xing)，每(mei)次(ci)(ci)實(shi)(shi)驗(yan)(yan)(yan)(yan)請使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)溶液(ye)(ye)(ye)(ye)(ye)(ye)。 2.棄(qi)去液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干，不(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)(xi)滌(di)(di)。每(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)作液(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)勻(yun)(yun)(yun)，在(zai)(zai)(zai)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一(yi)(yi)(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)鐘(zhong)(zhong)(zhong)。 3.溫(wen)(wen)育(yu)60分(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)，棄(qi)去孔(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干，洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)3次(ci)(ci)，每(mei)次(ci)(ci)浸泡1-2分(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)干。4.每(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)(su)工(gong)作液(ye)(ye)(ye)(ye)(ye)(ye)（同(tong)(tong)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)作液(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)鐘(zhong)(zhong)(zhong)。5.溫(wen)(wen)育(yu)60分(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)，棄(qi)去孔(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干，洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)5次(ci)(ci)，每(mei)次(ci)(ci)浸泡1-2分(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)干。6.依序(xu)(xu)(xu)每(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶液(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)(bi)光(guang)顯色（30分(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)，此(ci)時(shi)(shi)(shi)(shi)(shi)(shi)肉眼(yan)可(ke)(ke)見(jian)(jian)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)有(you)明顯的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)(du)(du)(du)(du)藍(lan)色，后(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)梯度(du)(du)(du)(du)(du)(du)(du)不(bu)(bu)(bu)(bu)明顯，即(ji)可(ke)(ke)終(zhong)止）。7.依序(xu)(xu)(xu)每(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止溶液(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止反(fan)(fan)應(ying)(ying)(ying)(ying)（此(ci)時(shi)(shi)(shi)(shi)(shi)(shi)藍(lan)色立轉黃(huang)(huang)色）。終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)順(shun)序(xu)(xu)(xu)應(ying)(ying)(ying)(ying)盡(jin)量(liang)與(yu)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)順(shun)序(xu)(xu)(xu)相(xiang)同(tong)(tong)。為(wei)(wei)了保(bao)(bao)(bao)證實(shi)(shi)驗(yan)(yan)(yan)(yan)結(jie)(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)，底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)(fan)應(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)間到后(hou)(hou)(hou)應(ying)(ying)(ying)(ying)盡(jin)快加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)聯儀在(zai)(zai)(zai)450nm波長依序(xu)(xu)(xu)測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)各孔(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)密(mi)度(du)(du)(du)(du)(du)(du)(du)（OD值(zhi)）。 在(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)內(nei)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)。植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)氫酶(mei)(mei)(mei)(mei)(mei)(CAT)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)注(zhu)意(yi)事(shi)項1.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)(zai)初次(ci)(ci)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)時(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)將(jiang)各種(zhong)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)管離(li)心(xin)數(shu)(shu)(shu)(shu)分(fen)(fen)鐘(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)集(ji)(ji)中(zhong)(zhong)到管底(di)。2.每(mei)次(ci)(ci)實(shi)(shi)驗(yan)(yan)(yan)(yan)留一(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)作為(wei)(wei)空(kong)白(bai)調(diao)零孔(kong)(kong)(kong)(kong)(kong)，該孔(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)任何試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，只是**加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶液(ye)(ye)(ye)(ye)(ye)(ye)及(ji)(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)時(shi)(shi)(shi)(shi)(shi)(shi)先用(yong)(yong)(yong)(yong)(yong)(yong)(yong)此(ci)孔(kong)(kong)(kong)(kong)(kong)調(diao)OD值(zhi)至零。 3.為(wei)(wei)防(fang)止樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)時(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)反(fan)(fan)應(ying)(ying)(ying)(ying)板(ban)(ban)(ban)(ban)(ban)放(fang)(fang)于鋪(pu)有(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密(mi)閉盒(he)內(nei)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)蓋(gai)或(huo)(huo)(huo)覆膜(mo)。 4.未(wei)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)或(huo)(huo)(huo)者試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，請于2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)作液(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)(su)工(gong)作液(ye)(ye)(ye)(ye)(ye)(ye)請依據(ju)所需(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)配(pei)(pei)(pei)置使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請勿(wu)重(zhong)復(fu)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)作液(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)、辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)(su)(su)工(gong)作液(ye)(ye)(ye)(ye)(ye)(ye)。 5.建議檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)均(jun)設(she)雙孔(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，以(yi)(yi)(yi)(yi)保(bao)(bao)(bao)證檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)。 植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)氫酶(mei)(mei)(mei)(mei)(mei)(CAT)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)方(fang)(fang)法(fa)手工(gong)洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)方(fang)(fang)法(fa)：吸(xi)去（不(bu)(bu)(bu)(bu)可(ke)(ke)觸及(ji)(ji)板(ban)(ban)(ban)(ban)(ban)壁(bi)(bi)）或(huo)(huo)(huo)甩(shuai)(shuai)掉酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)內(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)；在(zai)(zai)(zai)實(shi)(shi)驗(yan)(yan)(yan)(yan)臺上(shang)鋪(pu)墊(dian)幾(ji)層吸(xi)水紙(zhi)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)朝下(xia)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)力拍幾(ji)次(ci)(ci)；將(jiang)推(tui)薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)(xi)滌(di)(di)緩(huan)沖液(ye)(ye)(ye)(ye)(ye)(ye)至少(shao)0.3ml注(zhu)入(ru)(ru)孔(kong)(kong)(kong)(kong)(kong)內(nei)，浸泡1-2分(fen)(fen)鐘(zhong)(zhong)(zhong)。根(gen)(gen)(gen)(gen)據(ju)需(xu)(xu)(xu)要，重(zhong)復(fu)此(ci)過(guo)(guo)(guo)程(cheng)(cheng)數(shu)(shu)(shu)(shu)次(ci)(ci)。 自動洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)：如(ru)(ru)(ru)(ru)(ru)果(guo)(guo)有(you)自動洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)機，應(ying)(ying)(ying)(ying)在(zai)(zai)(zai)熟(shu)練(lian)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)再(zai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)到正式(shi)實(shi)(shi)驗(yan)(yan)(yan)(yan)過(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)。 計(ji)(ji)算(suan)   以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)(shu)(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)為(wei)(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通(tong)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)半對數(shu)(shu)(shu)(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)繪(hui)(hui)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)，根(gen)(gen)(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)查(cha)出(chu)(chu)(chu)相(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)；再(zai)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)計(ji)(ji)算(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)回歸(gui)(gui)方(fang)(fang)程(cheng)(cheng)式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代入(ru)(ru)方(fang)(fang)程(cheng)(cheng)式(shi)，計(ji)(ji)算(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)，再(zai)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)，即(ji)為(wei)(wei)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際(ji)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。 注(zhu)意(yi)事(shi)項1.當混(hun)合蛋白(bai)溶液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)盡(jin)量(liang)輕(qing)(qing)(qing)緩(huan)，避(bi)(bi)免起泡。2.洗(xi)(xi)(xi)滌(di)(di)過(guo)(guo)(guo)程(cheng)(cheng)非常重(zhong)要，不(bu)(bu)(bu)(bu)充(chong)分(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)(xi)滌(di)(di)易造成假陽性(xing)(xing)。3.一(yi)(yi)(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)間**控制(zhi)(zhi)(zhi)在(zai)(zai)(zai)5分(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)，如(ru)(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)(shu)(shu)(shu)量(liang)多，推(tui)薦使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)排(pai)槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)。4.請每(mei)次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)(tong)時(shi)(shi)(shi)(shi)(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)，做復(fu)孔(kong)(kong)(kong)(kong)(kong)。5.如(ru)(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中(zhong)(zhong)待測(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含(han)量(liang)過(guo)(guo)(guo)高，請先稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)再(zai)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，計(ji)(ji)算(suan)時(shi)(shi)(shi)(shi)(shi)(shi)請乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)。6.在(zai)(zai)(zai)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)溶液(ye)(ye)(ye)(ye)(ye)(ye)工(gong)作液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)，請以(yi)(yi)(yi)(yi)相(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)，不(bu)(bu)(bu)(bu)能(neng)混(hun)淆。7.底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請避(bi)(bi)光(guang)保(bao)(bao)(bao)存(cun)(cun)(cun)。 8.不(bu)(bu)(bu)(bu)要用(yong)(yong)(yong)(yong)(yong)(yong)(yong)其(qi)它(ta)生(sheng)(sheng)(sheng)(sheng)產廠家(jia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)替換(huan)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)。 注(zhu)意(yi)事(shi)項：收(shou)集(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)前(qian)(qian)(qian)(qian)必須清(qing)(qing)楚要檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成份是否足夠穩定(ding)(ding)(ding)。對收(shou)集(ji)(ji)后(hou)(hou)(hou)當天進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)，儲存(cun)(cun)(cun)在(zai)(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)(ru)(ru)(ru)有(you)特殊(shu)原因需(xu)(xu)(xu)要周(zhou)期(qi)收(shou)集(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)， 將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)及(ji)(ji)時(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)裝(zhuang)后(hou)(hou)(hou)放(fang)(fang)在(zai)(zai)(zai)-20 ℃或(huo)(huo)(huo)-70 ℃條件下(xia)保(bao)(bao)(bao)存(cun)(cun)(cun)。避(bi)(bi)免反(fan)(fan)復(fu)凍(dong)融。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)保(bao)(bao)(bao)存(cun)(cun)(cun)48 小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)保(bao)(bao)(bao)存(cun)(cun)(cun)1 個(ge)月(yue)(yue)。-70度(du)(du)(du)(du)(du)(du)(du)可(ke)(ke)保(bao)(bao)(bao)存(cun)(cun)(cun)6 個(ge)月(yue)(yue)。部(bu)分(fen)(fen)激素(su)(su)(su)類(lei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)需(xu)(xu)(xu)添加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)抑(yi)肽(tai)酶(mei)(mei)(mei)(mei)(mei)。計(ji)(ji)算(suan)：以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)為(wei)(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)繪(hui)(hui)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)，根(gen)(gen)(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)查(cha)出(chu)(chu)(chu)相(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)；再(zai)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)計(ji)(ji)算(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)回歸(gui)(gui)方(fang)(fang)程(cheng)(cheng)式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代入(ru)(ru)方(fang)(fang)程(cheng)(cheng)式(shi)，計(ji)(ji)算(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)，再(zai)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)，即(ji)為(wei)(wei)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際(ji)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。 植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)氫酶(mei)(mei)(mei)(mei)(mei)(CAT)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)性(xing)(xing)能(neng)1.樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)線(xian)(xian)性(xing)(xing)回歸(gui)(gui)與(yu)預(yu)期(qi)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)相(xiang)關(guan)系(xi)數(shu)(shu)(shu)(shu)R值(zhi)為(wei)(wei)0.92以(yi)(yi)(yi)(yi)上(shang)。2.批(pi)內(nei)與(yu)批(pi)見(jian)(jian)應(ying)(ying)(ying)(ying)分(fen)(fen)別小(xiao)(xiao)于9%和(he)(he)15% 植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)(hua)(hua)氫酶(mei)(mei)(mei)(mei)(mei)(CAT)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)

實(shi)(shi)(shi)(shi)驗(yan)(yan)原(yuan)(yuan)理   試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)采用(yong)(yong)(yong)(yong)雙抗(kang)體(ti)(ti)(ti)一步夾心法酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯免(mian)疫吸附試(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)（ELISA）。往預(yu)先(xian)(xian)(xian)包被過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)(POD)捕獲抗(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，依次(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)抗(kang)體(ti)(ti)(ti)，經過(guo)(guo)(guo)(guo)溫(wen)育并徹(che)底(di)洗(xi)(xi)滌。用(yong)(yong)(yong)(yong)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯(xian)(xian)(xian)色(se)(se)(se)，TMB在(zai)(zai)(zai)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)(hua)下(xia)(xia)(xia)(xia)轉(zhuan)化(hua)(hua)(hua)成藍色(se)(se)(se)，并在(zai)(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)下(xia)(xia)(xia)(xia)轉(zhuan)化(hua)(hua)(hua)成＊終的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)(se)(se)。顏色(se)(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深(shen)淺(qian)和(he)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)(POD)呈正(zheng)(zheng)相關(guan)。用(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)(zai)450nm 波長下(xia)(xia)(xia)(xia)測(ce)(ce)(ce)(ce)定(ding)(ding)吸光(guang)度(du)(du)(du)（OD 值(zhi)），計算(suan)(suan)(suan)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)。保(bao)存(cun)(cun)條(tiao)件及(ji)有效(xiao)(xiao)期(qi)(qi)1.本(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)用(yong)(yong)(yong)(yong)于(yu)體(ti)(ti)(ti)外定(ding)(ding)量(liang)(liang)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)血(xue)清(qing)、血(xue)漿、組織勻(yun)(yun)(yun)漿及(ji)相關(guan)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)(POD)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)；2.試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)保(bao)存(cun)(cun)：2-8℃；3.有效(xiao)(xiao)期(qi)(qi)：6個(ge)(ge)月。植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)(POD)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采集(ji)(ji)(ji)及(ji)保(bao)存(cun)(cun)1.血(xue)清(qing)：全血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)請(qing)于(yu)室溫(wen)放置(zhi)(zhi)2小時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)(huo)4℃過(guo)(guo)(guo)(guo)一晚(wan)后(hou)(hou)于(yu)1000 x g離心20分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，取(qu)上(shang)(shang)(shang)清(qing)即(ji)可檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放于(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)存(cun)(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)反(fan)復(fu)凍(dong)(dong)融(rong)(rong)。 2.血(xue)漿：可用(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)肝(gan)素(su)(su)(su)(su)作(zuo)(zuo)為(wei)(wei)(wei)抗(kang)凝劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)采集(ji)(ji)(ji)后(hou)(hou)30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)(nei)于(yu)2 - 8° C 1000 x g離心15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放于(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)存(cun)(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)反(fan)復(fu)凍(dong)(dong)融(rong)(rong)。 3.細胞培養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)清(qing)或(huo)(huo)(huo)(huo)其(qi)它生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)：1000 x g離心20分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，取(qu)上(shang)(shang)(shang)清(qing)即(ji)可檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放于(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)存(cun)(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)反(fan)復(fu)凍(dong)(dong)融(rong)(rong)。 (注(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)溶(rong)血(xue)會(hui)影響**檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)結(jie)果(guo)，因(yin)此溶(rong)血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)不宜(yi)進行此項(xiang)(xiang)(xiang)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)。) 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)(POD)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)   標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)則(ze)：首先(xian)(xian)(xian)通過(guo)(guo)(guo)(guo)文獻(xian)檢(jian)(jian)(jian)(jian)(jian)索(suo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)(fang)式(shi)了(le)(le)解(jie)待(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含量(liang)(liang)，確(que)定(ding)(ding)適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)。只(zhi)有稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍內(nei)(nei)(nei)(nei)(nei)(nei)，檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果(guo)才(cai)是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)，應(ying)(ying)(ying)(ying)(ying)(ying)做(zuo)好詳細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)錄。計算(suan)(suan)(suan)濃(nong)(nong)(nong)(nong)度(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)了(le)(le)“N”倍(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)應(ying)(ying)(ying)(ying)(ying)(ying)再乘以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)“N”。    標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)則(ze)：2瓶，每(mei)(mei)瓶臨用(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至1ml，蓋好后(hou)(hou)靜置(zhi)(zhi)10分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)上(shang)(shang)(shang)，然后(hou)(hou)反(fan)復(fu)顛倒/搓動(dong)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)助溶(rong)解(jie)，其(qi)濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)300 pg/ml，做(zuo)系列倍(bei)(bei)比(bi)(bi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)，分(fen)(fen)(fen)(fen)(fen)別稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)直(zhi)接作(zuo)(zuo)為(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)度(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)前(qian)(qian)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)(nei)配(pei)(pei)制(zhi)(zhi)。 如(ru)(ru)(ru)(ru)配(pei)(pei)制(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取(qu)0.5ml（不要(yao)少于(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)含0.5ml樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)(guan)中(zhong)(zhong)，混(hun)勻(yun)(yun)(yun)即(ji)可，其(qi)余濃(nong)(nong)(nong)(nong)度(du)(du)(du)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)此類推(tui)。    生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)則(ze)：臨用(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)根(gen)(gen)據(ju)(ju)(ju)預(yu)先(xian)(xian)(xian)計算(suan)(suan)(suan)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)次(ci)實(shi)(shi)(shi)(shi)驗(yan)(yan)所(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)配(pei)(pei)制(zhi)(zhi)（每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)(shi)際(ji)(ji)配(pei)(pei)制(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)多配(pei)(pei)制(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)(ru)10μl生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)例配(pei)(pei)制(zhi)(zhi)，輕(qing)輕(qing)混(hun)勻(yun)(yun)(yun)，在(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前(qian)(qian)一小時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)(nei)配(pei)(pei)制(zhi)(zhi)。    辣根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親和(he)素(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)則(ze)：臨用(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)辣根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親和(he)素(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)根(gen)(gen)據(ju)(ju)(ju)預(yu)先(xian)(xian)(xian)計算(suan)(suan)(suan)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)次(ci)實(shi)(shi)(shi)(shi)驗(yan)(yan)所(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)配(pei)(pei)制(zhi)(zhi)（每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)(shi)際(ji)(ji)配(pei)(pei)制(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)多配(pei)(pei)制(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)(ru)10μl辣根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親和(he)素(su)(su)(su)(su)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl辣根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親和(he)素(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)例配(pei)(pei)制(zhi)(zhi)，輕(qing)輕(qing)混(hun)勻(yun)(yun)(yun)，在(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前(qian)(qian)一小時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)(nei)配(pei)(pei)制(zhi)(zhi)。 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)(POD)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)操作(zuo)(zuo)步驟(zou)   實(shi)(shi)(shi)(shi)驗(yan)(yan)開始前(qian)(qian)，請(qing)提前(qian)(qian)配(pei)(pei)置(zhi)(zhi)好所(suo)有試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)或(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，均需(xu)混(hun)勻(yun)(yun)(yun)，混(hun)勻(yun)(yun)(yun)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盡量(liang)(liang)避(bi)(bi)免(mian)起泡(pao)(pao)(pao)(pao)。每(mei)(mei)次(ci)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)(ying)(ying)該做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)(xian)(xian)。如(ru)(ru)(ru)(ru)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)過(guo)(guo)(guo)(guo)高時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)進行稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)使(shi)(shi)(shi)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合(he)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)范圍。 1.加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)別設空白孔(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)、待(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)。空白孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)別加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)待(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)意(yi)不要(yao)有氣泡(pao)(pao)(pao)(pao)，加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板孔(kong)(kong)(kong)(kong)(kong)底(di)部，盡量(liang)(liang)不觸(chu)及(ji)孔(kong)(kong)(kong)(kong)(kong)壁，輕(qing)輕(qing)晃(huang)動(dong)混(hun)勻(yun)(yun)(yun)，酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)蓋或(huo)(huo)(huo)(huo)覆(fu)膜(mo)，37℃反(fan)應(ying)(ying)(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)保(bao)證(zheng)實(shi)(shi)(shi)(shi)驗(yan)(yan)結(jie)果(guo)有效(xiao)(xiao)性，每(mei)(mei)次(ci)實(shi)(shi)(shi)(shi)驗(yan)(yan)請(qing)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)。 2.棄去液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干(gan)(gan)(gan)，不用(yong)(yong)(yong)(yong)洗(xi)(xi)滌。每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)工(gong)(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取(qu)1μl生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)例配(pei)(pei)制(zhi)(zhi)，輕(qing)輕(qing)混(hun)勻(yun)(yun)(yun)，在(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前(qian)(qian)一小時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)(nei)配(pei)(pei)制(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。 3.溫(wen)育60分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)后(hou)(hou)，棄去孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干(gan)(gan)(gan)，洗(xi)(xi)板3次(ci)，每(mei)(mei)次(ci)浸泡(pao)(pao)(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)干(gan)(gan)(gan)。4.每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)辣根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親和(he)素(su)(su)(su)(su)工(gong)(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)（同生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)工(gong)(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。5.溫(wen)育60分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)后(hou)(hou)，棄去孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干(gan)(gan)(gan)，洗(xi)(xi)板5次(ci)，每(mei)(mei)次(ci)浸泡(pao)(pao)(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)干(gan)(gan)(gan)。6.依序(xu)(xu)每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)(bi)光(guang)顯(xian)(xian)(xian)色(se)(se)(se)（30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)(nei)，此時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)肉眼可見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)有明顯(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)藍色(se)(se)(se)，后(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)梯度(du)(du)(du)不明顯(xian)(xian)(xian)，即(ji)可終止(zhi)(zhi)）。7.依序(xu)(xu)每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終止(zhi)(zhi)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，終止(zhi)(zhi)反(fan)應(ying)(ying)(ying)(ying)(ying)(ying)（此時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)藍色(se)(se)(se)立轉(zhuan)黃色(se)(se)(se)）。終止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)順(shun)序(xu)(xu)應(ying)(ying)(ying)(ying)(ying)(ying)盡量(liang)(liang)與(yu)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)順(shun)序(xu)(xu)相同。為(wei)(wei)(wei)了(le)(le)保(bao)證(zheng)實(shi)(shi)(shi)(shi)驗(yan)(yan)結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性，底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)應(ying)(ying)(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)間(jian)(jian)到后(hou)(hou)應(ying)(ying)(ying)(ying)(ying)(ying)盡快(kuai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)終止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯儀在(zai)(zai)(zai)450nm波長依序(xu)(xu)測(ce)(ce)(ce)(ce)量(liang)(liang)各(ge)孔(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)密度(du)(du)(du)（OD值(zhi)）。 在(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)內(nei)(nei)(nei)(nei)(nei)(nei)進行檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)。植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)(POD)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)注(zhu)意(yi)事項(xiang)(xiang)(xiang)1.用(yong)(yong)(yong)(yong)戶在(zai)(zai)(zai)初次(ci)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)(ying)(ying)將(jiang)(jiang)(jiang)各(ge)種試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)管(guan)(guan)離心數(shu)(shu)(shu)(shu)分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)集(ji)(ji)(ji)中(zhong)(zhong)到管(guan)(guan)底(di)。2.每(mei)(mei)次(ci)實(shi)(shi)(shi)(shi)驗(yan)(yan)留一孔(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)為(wei)(wei)(wei)空白調零孔(kong)(kong)(kong)(kong)(kong)，該孔(kong)(kong)(kong)(kong)(kong)不加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)任何試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，只(zhi)是**加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)及(ji)2N H2SO4。測(ce)(ce)(ce)(ce)量(liang)(liang)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)先(xian)(xian)(xian)用(yong)(yong)(yong)(yong)此孔(kong)(kong)(kong)(kong)(kong)調OD值(zhi)至零。 3.為(wei)(wei)(wei)防(fang)止(zhi)(zhi)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)(jiang)反(fan)應(ying)(ying)(ying)(ying)(ying)(ying)板放于(yu)鋪有濕(shi)布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)(he)(he)(he)內(nei)(nei)(nei)(nei)(nei)(nei)，酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)蓋或(huo)(huo)(huo)(huo)覆(fu)膜(mo)。 4.未使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板或(huo)(huo)(huo)(huo)者試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，請(qing)于(yu)2-8℃保(bao)存(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)工(gong)(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)、辣根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親和(he)素(su)(su)(su)(su)工(gong)(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)請(qing)依據(ju)(ju)(ju)所(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)配(pei)(pei)置(zhi)(zhi)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)。請(qing)勿重(zhong)(zhong)復(fu)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)工(gong)(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)、辣根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親和(he)素(su)(su)(su)(su)工(gong)(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)。 5.建(jian)議(yi)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)均設雙孔(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)定(ding)(ding)，以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)保(bao)證(zheng)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性。 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)(POD)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)洗(xi)(xi)板方(fang)(fang)(fang)法手工(gong)(gong)(gong)(gong)洗(xi)(xi)板方(fang)(fang)(fang)法：吸去（不可觸(chu)及(ji)板壁）或(huo)(huo)(huo)(huo)甩(shuai)掉酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板內(nei)(nei)(nei)(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)；在(zai)(zai)(zai)實(shi)(shi)(shi)(shi)驗(yan)(yan)臺上(shang)(shang)(shang)鋪墊幾(ji)(ji)層吸水紙(zhi)(zhi)，酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板朝(chao)下(xia)(xia)(xia)(xia)用(yong)(yong)(yong)(yong)力拍幾(ji)(ji)次(ci)；將(jiang)(jiang)(jiang)推(tui)薦(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌緩沖液(ye)(ye)(ye)(ye)(ye)(ye)至少0.3ml注(zhu)入(ru)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)(nei)，浸泡(pao)(pao)(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。根(gen)(gen)據(ju)(ju)(ju)需(xu)要(yao)，重(zhong)(zhong)復(fu)此過(guo)(guo)(guo)(guo)程(cheng)數(shu)(shu)(shu)(shu)次(ci)。 自動(dong)洗(xi)(xi)板：如(ru)(ru)(ru)(ru)果(guo)有自動(dong)洗(xi)(xi)板機(ji)，應(ying)(ying)(ying)(ying)(ying)(ying)在(zai)(zai)(zai)熟練使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)后(hou)(hou)再用(yong)(yong)(yong)(yong)到正(zheng)(zheng)式(shi)實(shi)(shi)(shi)(shi)驗(yan)(yan)過(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)。 計算(suan)(suan)(suan)   以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)(shu)(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)為(wei)(wei)(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)半對數(shu)(shu)(shu)(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)(zhi)上(shang)(shang)(shang)繪(hui)出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)(xian)(xian)，根(gen)(gen)據(ju)(ju)(ju)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)(xian)(xian)查出相應(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)；再乘以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)與(yu)OD值(zhi)計算(suan)(suan)(suan)出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)(xian)(xian)(xian)(xian)回歸方(fang)(fang)(fang)程(cheng)式(shi)，將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代入(ru)方(fang)(fang)(fang)程(cheng)式(shi)，計算(suan)(suan)(suan)出樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)，再乘以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)，即(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)(shi)際(ji)(ji)濃(nong)(nong)(nong)(nong)度(du)(du)(du)。 注(zhu)意(yi)事項(xiang)(xiang)(xiang)1.當混(hun)合(he)蛋白溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)盡量(liang)(liang)輕(qing)緩，避(bi)(bi)免(mian)起泡(pao)(pao)(pao)(pao)。2.洗(xi)(xi)滌過(guo)(guo)(guo)(guo)程(cheng)非常(chang)重(zhong)(zhong)要(yao)，不充分(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌易造成假陽性。3.一次(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)間(jian)(jian)**控制(zhi)(zhi)在(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)(nei)，如(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)數(shu)(shu)(shu)(shu)量(liang)(liang)多，推(tui)薦(jian)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)。4.請(qing)每(mei)(mei)次(ci)測(ce)(ce)(ce)(ce)定(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)(xian)(xian)，做(zuo)復(fu)孔(kong)(kong)(kong)(kong)(kong)。5.如(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)待(dai)測(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)(liang)過(guo)(guo)(guo)(guo)高，請(qing)先(xian)(xian)(xian)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)再測(ce)(ce)(ce)(ce)定(ding)(ding)，計算(suan)(suan)(suan)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)請(qing)乘以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)。6.在(zai)(zai)(zai)配(pei)(pei)制(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)相應(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)制(zhi)(zhi)，不能混(hun)淆(xiao)。7.底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)避(bi)(bi)光(guang)保(bao)存(cun)(cun)。 8.不要(yao)用(yong)(yong)(yong)(yong)其(qi)它生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)廠(chang)家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)替換試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)。 注(zhu)意(yi)事項(xiang)(xiang)(xiang)：收(shou)集(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)前(qian)(qian)必須清(qing)楚要(yao)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成份是否足夠穩定(ding)(ding)。對收(shou)集(ji)(ji)(ji)后(hou)(hou)當天進行檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)，儲存(cun)(cun)在(zai)(zai)(zai)4 ℃備(bei)用(yong)(yong)(yong)(yong)，如(ru)(ru)(ru)(ru)有特殊(shu)原(yuan)(yuan)因(yin)需(xu)要(yao)周期(qi)(qi)收(shou)集(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)， 將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)及(ji)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)裝后(hou)(hou)放在(zai)(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)-70 ℃條(tiao)件下(xia)(xia)(xia)(xia)保(bao)存(cun)(cun)。避(bi)(bi)免(mian)反(fan)復(fu)凍(dong)(dong)融(rong)(rong)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)2 -8 ℃可保(bao)存(cun)(cun)48 小時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可保(bao)存(cun)(cun)1 個(ge)(ge)月。-70度(du)(du)(du)可保(bao)存(cun)(cun)6 個(ge)(ge)月。部分(fen)(fen)(fen)(fen)(fen)激素(su)(su)(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)需(xu)添加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)抑(yi)肽酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)。計算(suan)(suan)(suan)：以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)為(wei)(wei)(wei)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)(zhi)上(shang)(shang)(shang)繪(hui)出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)(xian)(xian)，根(gen)(gen)據(ju)(ju)(ju)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)(xian)(xian)查出相應(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)；再乘以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)與(yu)OD值(zhi)計算(suan)(suan)(suan)出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)(xian)(xian)(xian)(xian)回歸方(fang)(fang)(fang)程(cheng)式(shi)，將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代入(ru)方(fang)(fang)(fang)程(cheng)式(shi)，計算(suan)(suan)(suan)出樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)，再乘以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)，即(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)(shi)際(ji)(ji)濃(nong)(nong)(nong)(nong)度(du)(du)(du)。 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)(POD)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)性能1.樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)(xian)(xian)(xian)性回歸與(yu)預(yu)期(qi)(qi)濃(nong)(nong)(nong)(nong)度(du)(du)(du)相關(guan)系數(shu)(shu)(shu)(shu)R值(zhi)為(wei)(wei)(wei)0.92以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)上(shang)(shang)(shang)。2.批(pi)內(nei)(nei)(nei)(nei)(nei)(nei)與(yu)批(pi)見應(ying)(ying)(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)(fen)別小于(yu)9%和(he)15% 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)過(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)(POD)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)

實(shi)(shi)驗(yan)(yan)(yan)原(yuan)理(li)   試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)一步(bu)夾心法酶(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)疫吸附試(shi)(shi)(shi)驗(yan)(yan)(yan)（ELISA）。往預(yu)(yu)先包(bao)被超(chao)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)歧(qi)化(hua)(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(SOD)捕獲抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包(bao)被微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，依次(ci)(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)，經過(guo)(guo)(guo)(guo)(guo)(guo)溫(wen)育并徹(che)底(di)(di)洗(xi)(xi)滌(di)(di)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯色(se)(se)(se)，TMB在(zai)(zai)(zai)(zai)(zai)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)(hua)(hua)(hua)下轉(zhuan)化(hua)(hua)(hua)(hua)(hua)成(cheng)藍色(se)(se)(se)，并在(zai)(zai)(zai)(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)下轉(zhuan)化(hua)(hua)(hua)(hua)(hua)成(cheng)＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)色(se)(se)(se)。顏色(se)(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)超(chao)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)歧(qi)化(hua)(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(SOD)呈正相(xiang)(xiang)關(guan)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)(zai)(zai)(zai)450nm 波(bo)(bo)長(chang)下測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)吸光(guang)度(du)(du)(du)（OD 值(zhi)(zhi)(zhi)(zhi)），計(ji)(ji)(ji)(ji)(ji)算樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)。保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)條件及有(you)效(xiao)期(qi)1.本(ben)(ben)(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)體(ti)(ti)(ti)(ti)(ti)外定(ding)量(liang)(liang)(liang)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)血(xue)(xue)清(qing)、血(xue)(xue)漿(jiang)、組織勻(yun)漿(jiang)及相(xiang)(xiang)關(guan)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)中(zhong)超(chao)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)歧(qi)化(hua)(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(SOD)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)(liang)；2.試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)：2-8℃；3.有(you)效(xiao)期(qi)：6個(ge)月。植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)超(chao)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)歧(qi)化(hua)(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(SOD)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集(ji)(ji)(ji)及保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)1.血(xue)(xue)清(qing)：全血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)請(qing)(qing)于(yu)(yu)(yu)室(shi)溫(wen)放置(zhi)2小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)(huo)(huo)(huo)4℃過(guo)(guo)(guo)(guo)(guo)(guo)一晚后(hou)(hou)(hou)(hou)于(yu)(yu)(yu)1000 x g離心20分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，取上(shang)(shang)(shang)清(qing)即可檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)，但應(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)反(fan)(fan)(fan)復凍融(rong)。 2.血(xue)(xue)漿(jiang)：可用(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)(huo)(huo)肝素(su)(su)作(zuo)(zuo)為(wei)(wei)(wei)(wei)抗(kang)(kang)(kang)(kang)(kang)凝劑(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)采(cai)集(ji)(ji)(ji)后(hou)(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)于(yu)(yu)(yu)2 - 8° C 1000 x g離心15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)，但應(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)反(fan)(fan)(fan)復凍融(rong)。 3.細胞培養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)清(qing)或(huo)(huo)(huo)(huo)(huo)(huo)其(qi)它生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)：1000 x g離心20分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，取上(shang)(shang)(shang)清(qing)即可檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)放于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)，但應(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)反(fan)(fan)(fan)復凍融(rong)。 (注(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)溶(rong)(rong)(rong)血(xue)(xue)會影響(xiang)**檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)(guo)，因此(ci)溶(rong)(rong)(rong)血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)不(bu)(bu)(bu)宜進行此(ci)項檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)。) 植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)超(chao)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)歧(qi)化(hua)(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(SOD)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)   標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)(ze)：首先通過(guo)(guo)(guo)(guo)(guo)(guo)文獻檢(jian)(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)式(shi)了解(jie)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含量(liang)(liang)(liang)，確(que)定(ding)適當(dang)(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)。只有(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)至(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍(wei)(wei)內(nei)(nei)，檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果(guo)(guo)才是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)中(zhong)，應(ying)(ying)(ying)(ying)做好(hao)詳細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)錄。計(ji)(ji)(ji)(ji)(ji)算濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)應(ying)(ying)(ying)(ying)再(zai)乘(cheng)(cheng)(cheng)以(yi)(yi)“N”。    標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)(ze)：2瓶，每(mei)(mei)瓶臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)至(zhi)(zhi)(zhi)1ml，蓋(gai)好(hao)后(hou)(hou)(hou)(hou)靜置(zhi)10分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)上(shang)(shang)(shang)，然后(hou)(hou)(hou)(hou)反(fan)(fan)(fan)復顛倒(dao)/搓動(dong)以(yi)(yi)助溶(rong)(rong)(rong)解(jie)，其(qi)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)(wei)300 pg/ml，做系列倍(bei)比(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)直(zhi)接(jie)作(zuo)(zuo)為(wei)(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)。 如(ru)(ru)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取0.5ml（不(bu)(bu)(bu)要(yao)少于(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)含0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中(zhong)，混(hun)勻(yun)即可，其(qi)余濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)以(yi)(yi)此(ci)類推(tui)。    生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)(ze)：臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以(yi)(yi)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)根(gen)(gen)(gen)(gen)據(ju)(ju)(ju)預(yu)(yu)先計(ji)(ji)(ji)(ji)(ji)算好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)次(ci)(ci)(ci)(ci)(ci)(ci)實(shi)(shi)驗(yan)(yan)(yan)所(suo)(suo)需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)（每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)際(ji)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)多配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混(hun)勻(yun)，在(zai)(zai)(zai)(zai)(zai)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)。    辣根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)素(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)(ze)：臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以(yi)(yi)辣根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)素(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)根(gen)(gen)(gen)(gen)據(ju)(ju)(ju)預(yu)(yu)先計(ji)(ji)(ji)(ji)(ji)算好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)次(ci)(ci)(ci)(ci)(ci)(ci)實(shi)(shi)驗(yan)(yan)(yan)所(suo)(suo)需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)（每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)際(ji)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)多配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl辣根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)素(su)(su)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl辣根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)素(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混(hun)勻(yun)，在(zai)(zai)(zai)(zai)(zai)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)。 植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)超(chao)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)歧(qi)化(hua)(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(SOD)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)操作(zuo)(zuo)步(bu)驟   實(shi)(shi)驗(yan)(yan)(yan)開始前(qian)(qian)(qian)(qian)，請(qing)(qing)提前(qian)(qian)(qian)(qian)配(pei)(pei)(pei)(pei)(pei)置(zhi)好(hao)所(suo)(suo)有(you)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)或(huo)(huo)(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，均(jun)需(xu)(xu)混(hun)勻(yun)，混(hun)勻(yun)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盡量(liang)(liang)(liang)避(bi)(bi)免(mian)(mian)起泡(pao)(pao)。每(mei)(mei)次(ci)(ci)(ci)(ci)(ci)(ci)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)該(gai)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)。如(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)過(guo)(guo)(guo)(guo)(guo)(guo)高時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)進行稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)使樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合(he)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)范圍(wei)(wei)。 1.加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)設(she)空(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)(kong)、待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)。空(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)(huo)(huo)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)意不(bu)(bu)(bu)要(yao)有(you)氣(qi)泡(pao)(pao)，加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)(yu)(yu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)底(di)(di)部，盡量(liang)(liang)(liang)不(bu)(bu)(bu)觸及孔(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)(qing)輕(qing)(qing)晃動(dong)混(hun)勻(yun)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)蓋(gai)或(huo)(huo)(huo)(huo)(huo)(huo)覆(fu)膜，37℃反(fan)(fan)(fan)應(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)(wei)保(bao)(bao)(bao)(bao)證實(shi)(shi)驗(yan)(yan)(yan)結(jie)果(guo)(guo)有(you)效(xiao)性，每(mei)(mei)次(ci)(ci)(ci)(ci)(ci)(ci)實(shi)(shi)驗(yan)(yan)(yan)請(qing)(qing)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)新(xin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 2.棄(qi)去液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩(shuai)(shuai)(shuai)干，不(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)滌(di)(di)。每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混(hun)勻(yun)，在(zai)(zai)(zai)(zai)(zai)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。 3.溫(wen)育60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)，棄(qi)去孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩(shuai)(shuai)(shuai)干，洗(xi)(xi)板(ban)(ban)3次(ci)(ci)(ci)(ci)(ci)(ci)，每(mei)(mei)次(ci)(ci)(ci)(ci)(ci)(ci)浸(jin)泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)(shuai)干。4.每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)辣根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)素(su)(su)工作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同(tong)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。5.溫(wen)育60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)，棄(qi)去孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩(shuai)(shuai)(shuai)干，洗(xi)(xi)板(ban)(ban)5次(ci)(ci)(ci)(ci)(ci)(ci)，每(mei)(mei)次(ci)(ci)(ci)(ci)(ci)(ci)浸(jin)泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)(shuai)干。6.依序(xu)每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)(bi)光(guang)顯色(se)(se)(se)（30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)，此(ci)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)肉(rou)眼可見(jian)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)有(you)明顯的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)藍色(se)(se)(se)，后(hou)(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)梯度(du)(du)(du)不(bu)(bu)(bu)明顯，即可終(zhong)止）。7.依序(xu)每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止反(fan)(fan)(fan)應(ying)(ying)(ying)(ying)（此(ci)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)藍色(se)(se)(se)立轉(zhuan)黃(huang)色(se)(se)(se)）。終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)順序(xu)應(ying)(ying)(ying)(ying)盡量(liang)(liang)(liang)與底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)順序(xu)相(xiang)(xiang)同(tong)。為(wei)(wei)(wei)(wei)了保(bao)(bao)(bao)(bao)證實(shi)(shi)驗(yan)(yan)(yan)結(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性，底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)(fan)(fan)應(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)間(jian)到(dao)后(hou)(hou)(hou)(hou)應(ying)(ying)(ying)(ying)盡快加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)聯(lian)儀在(zai)(zai)(zai)(zai)(zai)450nm波(bo)(bo)長(chang)依序(xu)測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)各孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)密(mi)度(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)）。 在(zai)(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)內(nei)(nei)進行檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)。植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)超(chao)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)歧(qi)化(hua)(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(SOD)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)注(zhu)意事項1.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)(zai)(zai)(zai)初次(ci)(ci)(ci)(ci)(ci)(ci)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)將(jiang)各種(zhong)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)管離心數(shu)(shu)(shu)(shu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，以(yi)(yi)便試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)集(ji)(ji)(ji)中(zhong)到(dao)管底(di)(di)。2.每(mei)(mei)次(ci)(ci)(ci)(ci)(ci)(ci)實(shi)(shi)驗(yan)(yan)(yan)留一孔(kong)(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)為(wei)(wei)(wei)(wei)空(kong)白調零孔(kong)(kong)(kong)(kong)(kong)(kong)，該(gai)孔(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)任何試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，只是**加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)及2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)先用(yong)(yong)(yong)(yong)(yong)(yong)(yong)此(ci)孔(kong)(kong)(kong)(kong)(kong)(kong)調OD值(zhi)(zhi)(zhi)(zhi)至(zhi)(zhi)(zhi)零。 3.為(wei)(wei)(wei)(wei)防止樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)驗(yan)(yan)(yan)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)反(fan)(fan)(fan)應(ying)(ying)(ying)(ying)板(ban)(ban)放于(yu)(yu)(yu)鋪(pu)有(you)濕布(bu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密(mi)閉盒(he)(he)(he)內(nei)(nei)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)蓋(gai)或(huo)(huo)(huo)(huo)(huo)(huo)覆(fu)膜。 4.未使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)或(huo)(huo)(huo)(huo)(huo)(huo)者試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，請(qing)(qing)于(yu)(yu)(yu)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)素(su)(su)工作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)請(qing)(qing)依據(ju)(ju)(ju)所(suo)(suo)需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)(pei)置(zhi)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)勿重復使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)(huo)(huo)、辣根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)素(su)(su)工作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5.建議檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)均(jun)設(she)雙孔(kong)(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)，以(yi)(yi)保(bao)(bao)(bao)(bao)證檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性。 植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)超(chao)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)歧(qi)化(hua)(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(SOD)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)洗(xi)(xi)板(ban)(ban)方(fang)法手工洗(xi)(xi)板(ban)(ban)方(fang)法：吸去（不(bu)(bu)(bu)可觸及板(ban)(ban)壁）或(huo)(huo)(huo)(huo)(huo)(huo)甩(shuai)(shuai)(shuai)掉酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)內(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)；在(zai)(zai)(zai)(zai)(zai)實(shi)(shi)驗(yan)(yan)(yan)臺上(shang)(shang)(shang)鋪(pu)墊幾(ji)層吸水紙，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)朝(chao)下用(yong)(yong)(yong)(yong)(yong)(yong)(yong)力拍幾(ji)次(ci)(ci)(ci)(ci)(ci)(ci)；將(jiang)推(tui)薦(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)(di)緩沖液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)至(zhi)(zhi)(zhi)少0.3ml注(zhu)入(ru)(ru)(ru)孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)，浸(jin)泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。根(gen)(gen)(gen)(gen)據(ju)(ju)(ju)需(xu)(xu)要(yao)，重復此(ci)過(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)數(shu)(shu)(shu)(shu)次(ci)(ci)(ci)(ci)(ci)(ci)。 自動(dong)洗(xi)(xi)板(ban)(ban)：如(ru)(ru)果(guo)(guo)有(you)自動(dong)洗(xi)(xi)板(ban)(ban)機，應(ying)(ying)(ying)(ying)在(zai)(zai)(zai)(zai)(zai)熟練使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)(hou)再(zai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)到(dao)正式(shi)實(shi)(shi)驗(yan)(yan)(yan)過(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)中(zhong)。 計(ji)(ji)(ji)(ji)(ji)算   以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)(wei)橫(heng)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)(shu)(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)(zai)(zai)半對數(shu)(shu)(shu)(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)繪(hui)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)，根(gen)(gen)(gen)(gen)據(ju)(ju)(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)查出(chu)相(xiang)(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)；再(zai)乘(cheng)(cheng)(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)與OD值(zhi)(zhi)(zhi)(zhi)計(ji)(ji)(ji)(ji)(ji)算出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)回(hui)歸方(fang)程(cheng)(cheng)(cheng)式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)代入(ru)(ru)(ru)方(fang)程(cheng)(cheng)(cheng)式(shi)，計(ji)(ji)(ji)(ji)(ji)算出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)，再(zai)乘(cheng)(cheng)(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)，即為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際(ji)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)。 注(zhu)意事項1.當(dang)(dang)混(hun)合(he)蛋白溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)盡量(liang)(liang)(liang)輕(qing)(qing)緩，避(bi)(bi)免(mian)(mian)起泡(pao)(pao)。2.洗(xi)(xi)滌(di)(di)過(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)非常重要(yao)，不(bu)(bu)(bu)充分(fen)(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)(di)易(yi)造成(cheng)假陽性。3.一次(ci)(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)間(jian)**控制(zhi)(zhi)在(zai)(zai)(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)數(shu)(shu)(shu)(shu)量(liang)(liang)(liang)多，推(tui)薦(jian)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)排槍(qiang)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4.請(qing)(qing)每(mei)(mei)次(ci)(ci)(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)，做復孔(kong)(kong)(kong)(kong)(kong)(kong)。5.如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)中(zhong)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)(liang)(liang)過(guo)(guo)(guo)(guo)(guo)(guo)高，請(qing)(qing)先稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)再(zai)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)，計(ji)(ji)(ji)(ji)(ji)算時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)請(qing)(qing)乘(cheng)(cheng)(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)。6.在(zai)(zai)(zai)(zai)(zai)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)工作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)(qing)以(yi)(yi)相(xiang)(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)，不(bu)(bu)(bu)能混(hun)淆。7.底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)避(bi)(bi)光(guang)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。 8.不(bu)(bu)(bu)要(yao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)其(qi)它生(sheng)(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)替換試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)。 注(zhu)意事項：收集(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)前(qian)(qian)(qian)(qian)必須(xu)清(qing)楚要(yao)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)份是否足(zu)夠穩定(ding)。對收集(ji)(ji)(ji)后(hou)(hou)(hou)(hou)當(dang)(dang)天進行檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)，儲存(cun)(cun)(cun)在(zai)(zai)(zai)(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)有(you)特殊原(yuan)因需(xu)(xu)要(yao)周(zhou)期(qi)收集(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)， 將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)及時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝后(hou)(hou)(hou)(hou)放在(zai)(zai)(zai)(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)(huo)(huo)-70 ℃條件下保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。避(bi)(bi)免(mian)(mian)反(fan)(fan)(fan)復凍融(rong)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)2 -8 ℃可保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)48 小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)1 個(ge)月。-70度(du)(du)(du)可保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)6 個(ge)月。部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)激素(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)需(xu)(xu)添加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)抑肽酶(mei)(mei)(mei)(mei)(mei)。計(ji)(ji)(ji)(ji)(ji)算：以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)(wei)橫(heng)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)(zai)(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)繪(hui)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)，根(gen)(gen)(gen)(gen)據(ju)(ju)(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)查出(chu)相(xiang)(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)；再(zai)乘(cheng)(cheng)(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)與OD值(zhi)(zhi)(zhi)(zhi)計(ji)(ji)(ji)(ji)(ji)算出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)回(hui)歸方(fang)程(cheng)(cheng)(cheng)式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)代入(ru)(ru)(ru)方(fang)程(cheng)(cheng)(cheng)式(shi)，計(ji)(ji)(ji)(ji)(ji)算出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)，再(zai)乘(cheng)(cheng)(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)，即為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際(ji)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)。 植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)超(chao)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)歧(qi)化(hua)(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(SOD)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)性能1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)性回(hui)歸與預(yu)(yu)期(qi)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)相(xiang)(xiang)關(guan)系數(shu)(shu)(shu)(shu)R值(zhi)(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)0.92以(yi)(yi)上(shang)(shang)(shang)。2.批內(nei)(nei)與批見(jian)應(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)小(xiao)于(yu)(yu)(yu)9%和(he)(he)15% 植(zhi)(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)超(chao)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)歧(qi)化(hua)(hua)(hua)(hua)(hua)酶(mei)(mei)(mei)(mei)(mei)(SOD)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)

實(shi)驗(yan)(yan)原理   試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)采用(yong)(yong)(yong)(yong)(yong)雙(shuang)抗(kang)(kang)(kang)體(ti)(ti)一(yi)步夾(jia)心(xin)法酶(mei)(mei)(mei)(mei)(mei)聯免(mian)(mian)疫(yi)吸附試(shi)(shi)(shi)(shi)驗(yan)(yan)（ELISA）。往(wang)預先(xian)(xian)包被激(ji)(ji)(ji)(ji)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)脫(tuo)(tuo)落(luo)(luo)(luo)(luo)(luo)酸(suan)(ABA)捕獲抗(kang)(kang)(kang)體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，依次(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)體(ti)(ti)，經過(guo)(guo)(guo)溫(wen)育(yu)(yu)并徹底洗(xi)(xi)滌(di)(di)。用(yong)(yong)(yong)(yong)(yong)底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯色(se)(se)(se)，TMB在(zai)(zai)(zai)(zai)過(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)(hua)下轉(zhuan)化(hua)(hua)(hua)成藍(lan)色(se)(se)(se)，并在(zai)(zai)(zai)(zai)酸(suan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)下轉(zhuan)化(hua)(hua)(hua)成＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)(se)(se)。顏色(se)(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)激(ji)(ji)(ji)(ji)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)脫(tuo)(tuo)落(luo)(luo)(luo)(luo)(luo)酸(suan)(ABA)呈(cheng)正相(xiang)關。用(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)(zai)(zai)450nm 波(bo)長下測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)吸光(guang)度(du)(du)(du)(du)(du)(du)(du)（OD 值(zhi)），計(ji)(ji)(ji)(ji)算樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)條(tiao)件(jian)及(ji)(ji)(ji)(ji)有(you)效期1.本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)用(yong)(yong)(yong)(yong)(yong)于體(ti)(ti)外定(ding)量檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)血(xue)清(qing)、血(xue)漿、組織勻(yun)漿及(ji)(ji)(ji)(ji)相(xiang)關液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)中(zhong)激(ji)(ji)(ji)(ji)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)脫(tuo)(tuo)落(luo)(luo)(luo)(luo)(luo)酸(suan)(ABA)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)量；2.試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)：2-8℃；3.有(you)效期：6個月。植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)激(ji)(ji)(ji)(ji)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)脫(tuo)(tuo)落(luo)(luo)(luo)(luo)(luo)酸(suan)(ABA)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采集及(ji)(ji)(ji)(ji)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)1.血(xue)清(qing)：全血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)請(qing)(qing)于室(shi)溫(wen)放(fang)(fang)置(zhi)2小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)4℃過(guo)(guo)(guo)一(yi)晚后(hou)(hou)(hou)于1000 x g離心(xin)20分(fen)(fen)鐘(zhong)(zhong)，取上(shang)(shang)(shang)清(qing)即(ji)(ji)可(ke)(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)(fang)于-20℃或(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)反(fan)復(fu)凍融。 2.血(xue)漿：可(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)肝(gan)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)作(zuo)(zuo)為(wei)(wei)(wei)抗(kang)(kang)(kang)凝劑(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)采集后(hou)(hou)(hou)30分(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)(nei)于2 - 8° C 1000 x g離心(xin)15分(fen)(fen)鐘(zhong)(zhong)，或(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)(fang)于-20℃或(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)反(fan)復(fu)凍融。 3.細(xi)胞培養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)清(qing)或(huo)(huo)其(qi)(qi)它(ta)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)：1000 x g離心(xin)20分(fen)(fen)鐘(zhong)(zhong)，取上(shang)(shang)(shang)清(qing)即(ji)(ji)可(ke)(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)(fang)于-20℃或(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)反(fan)復(fu)凍融。 (注(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)溶(rong)(rong)血(xue)會影響**檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)結果，因此(ci)溶(rong)(rong)血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)不(bu)(bu)宜進(jin)(jin)行(xing)(xing)此(ci)項檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)。) 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)激(ji)(ji)(ji)(ji)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)脫(tuo)(tuo)落(luo)(luo)(luo)(luo)(luo)酸(suan)(ABA)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)   標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則：首先(xian)(xian)通過(guo)(guo)(guo)文獻檢(jian)(jian)(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)式(shi)(shi)(shi)了解待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含(han)量，確(que)定(ding)適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)。只(zhi)有(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍內(nei)(nei)(nei)(nei)，檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結果才是(shi)準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)，應(ying)(ying)(ying)(ying)(ying)(ying)做好詳細(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)(ji)錄(lu)。計(ji)(ji)(ji)(ji)算濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)應(ying)(ying)(ying)(ying)(ying)(ying)再(zai)(zai)乘(cheng)以(yi)(yi)(yi)“N”。    標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則：2瓶，每(mei)(mei)(mei)瓶臨(lin)(lin)(lin)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至1ml，蓋好后(hou)(hou)(hou)靜置(zhi)10分(fen)(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)上(shang)(shang)(shang)，然后(hou)(hou)(hou)反(fan)復(fu)顛倒/搓動以(yi)(yi)(yi)助溶(rong)(rong)解，其(qi)(qi)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)300 pg/ml，做系列倍(bei)(bei)比(bi)(bi)(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)，分(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)直(zhi)接作(zuo)(zuo)為(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)0 pg/ml，臨(lin)(lin)(lin)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)15分(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)。 如(ru)(ru)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)：取0.5ml（不(bu)(bu)要(yao)(yao)少于0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)含(han)0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)中(zhong)，混勻(yun)即(ji)(ji)可(ke)(ke)(ke)，其(qi)(qi)余(yu)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)以(yi)(yi)(yi)此(ci)類(lei)推(tui)。    生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則：臨(lin)(lin)(lin)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以(yi)(yi)(yi)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)根據(ju)預先(xian)(xian)計(ji)(ji)(ji)(ji)算好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)驗(yan)(yan)所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)多(duo)(duo)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)0.1-0.2ml。如(ru)(ru)10μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)加(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(li)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)，輕(qing)(qing)輕(qing)(qing)混勻(yun)，在(zai)(zai)(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一(yi)小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)。    辣(la)(la)根過(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則：臨(lin)(lin)(lin)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以(yi)(yi)(yi)辣(la)(la)根過(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)根據(ju)預先(xian)(xian)計(ji)(ji)(ji)(ji)算好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)驗(yan)(yan)所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)多(duo)(duo)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)0.1-0.2ml。如(ru)(ru)10μl辣(la)(la)根過(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)加(jia)(jia)(jia)(jia)(jia)990μl辣(la)(la)根過(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(li)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)，輕(qing)(qing)輕(qing)(qing)混勻(yun)，在(zai)(zai)(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一(yi)小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)。 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)激(ji)(ji)(ji)(ji)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)脫(tuo)(tuo)落(luo)(luo)(luo)(luo)(luo)酸(suan)(ABA)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)操作(zuo)(zuo)步驟   實(shi)驗(yan)(yan)開(kai)始(shi)前(qian)(qian)(qian)(qian)，請(qing)(qing)提前(qian)(qian)(qian)(qian)配(pei)(pei)(pei)(pei)(pei)(pei)置(zhi)好所有(you)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)或(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)，均需(xu)混勻(yun)，混勻(yun)時(shi)(shi)(shi)(shi)(shi)(shi)盡(jin)量避(bi)(bi)免(mian)(mian)起(qi)泡(pao)。每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)(ying)(ying)該做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)。如(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)過(guo)(guo)(guo)高時(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)進(jin)(jin)行(xing)(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)使(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)符合試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)范圍。 1.加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)別(bie)設(she)(she)空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)、待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余(yu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)或(huo)(huo)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)100μl，注(zhu)意(yi)不(bu)(bu)要(yao)(yao)有(you)氣泡(pao)，加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)于酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部，盡(jin)量不(bu)(bu)觸(chu)及(ji)(ji)(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)(qing)輕(qing)(qing)晃動混勻(yun)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)加(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)蓋或(huo)(huo)覆(fu)膜，37℃反(fan)應(ying)(ying)(ying)(ying)(ying)(ying)120分(fen)(fen)鐘(zhong)(zhong)。 為(wei)(wei)(wei)保(bao)(bao)(bao)(bao)(bao)證(zheng)(zheng)實(shi)驗(yan)(yan)結果有(you)效性(xing)(xing)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)驗(yan)(yan)請(qing)(qing)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 2.棄(qi)去液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，甩(shuai)干(gan)，不(bu)(bu)用(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)滌(di)(di)。每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)加(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(li)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)，輕(qing)(qing)輕(qing)(qing)混勻(yun)，在(zai)(zai)(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一(yi)小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)），37℃,60分(fen)(fen)鐘(zhong)(zhong)。 3.溫(wen)育(yu)(yu)60分(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)(hou)，棄(qi)去孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，甩(shuai)干(gan)，洗(xi)(xi)板(ban)3次(ci)(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)浸(jin)泡(pao)1-2分(fen)(fen)鐘(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)干(gan)。4.每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)辣(la)(la)根過(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同(tong)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)鐘(zhong)(zhong)。5.溫(wen)育(yu)(yu)60分(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)(hou)，棄(qi)去孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，甩(shuai)干(gan)，洗(xi)(xi)板(ban)5次(ci)(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)浸(jin)泡(pao)1-2分(fen)(fen)鐘(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)干(gan)。6.依序每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)(bi)光(guang)顯色(se)(se)(se)（30分(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)(nei)，此(ci)時(shi)(shi)(shi)(shi)(shi)(shi)肉眼可(ke)(ke)(ke)見(jian)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)有(you)明(ming)顯的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)(du)(du)(du)(du)藍(lan)色(se)(se)(se)，后(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)梯度(du)(du)(du)(du)(du)(du)(du)不(bu)(bu)明(ming)顯，即(ji)(ji)可(ke)(ke)(ke)終(zhong)止）。7.依序每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)終(zhong)止溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止反(fan)應(ying)(ying)(ying)(ying)(ying)(ying)（此(ci)時(shi)(shi)(shi)(shi)(shi)(shi)藍(lan)色(se)(se)(se)立轉(zhuan)黃色(se)(se)(se)）。終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)順序應(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)量與底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)順序相(xiang)同(tong)。為(wei)(wei)(wei)了保(bao)(bao)(bao)(bao)(bao)證(zheng)(zheng)實(shi)驗(yan)(yan)結果的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)，底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)應(ying)(ying)(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)間(jian)到后(hou)(hou)(hou)應(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)快加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)聯儀在(zai)(zai)(zai)(zai)450nm波(bo)長依序測(ce)(ce)(ce)(ce)(ce)(ce)(ce)量各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)密度(du)(du)(du)(du)(du)(du)(du)（OD值(zhi)）。 在(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)內(nei)(nei)(nei)(nei)進(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)。植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)激(ji)(ji)(ji)(ji)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)脫(tuo)(tuo)落(luo)(luo)(luo)(luo)(luo)酸(suan)(ABA)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)注(zhu)意(yi)事(shi)(shi)項1.用(yong)(yong)(yong)(yong)(yong)戶(hu)在(zai)(zai)(zai)(zai)初次(ci)(ci)(ci)(ci)(ci)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)時(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)(ying)(ying)將(jiang)(jiang)(jiang)(jiang)各種試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)管(guan)離心(xin)數(shu)分(fen)(fen)鐘(zhong)(zhong)，以(yi)(yi)(yi)便(bian)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)集中(zhong)到管(guan)底。2.每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)驗(yan)(yan)留一(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)為(wei)(wei)(wei)空白(bai)調零(ling)(ling)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，該孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)(jia)任何(he)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)，只(zhi)是(shi)**加(jia)(jia)(jia)(jia)(jia)底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)及(ji)(ji)(ji)(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)量時(shi)(shi)(shi)(shi)(shi)(shi)先(xian)(xian)用(yong)(yong)(yong)(yong)(yong)此(ci)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)調OD值(zhi)至零(ling)(ling)。 3.為(wei)(wei)(wei)防止樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)蒸發(fa)，試(shi)(shi)(shi)(shi)驗(yan)(yan)時(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)(jiang)(jiang)反(fan)應(ying)(ying)(ying)(ying)(ying)(ying)板(ban)放(fang)(fang)于鋪(pu)有(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)內(nei)(nei)(nei)(nei)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)加(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)蓋或(huo)(huo)覆(fu)膜。 4.未(wei)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)或(huo)(huo)者試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)，請(qing)(qing)于2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)(la)根過(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)請(qing)(qing)依據(ju)所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量配(pei)(pei)(pei)(pei)(pei)(pei)置(zhi)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)勿重復(fu)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)已(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)、辣(la)(la)根過(guo)(guo)(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5.建議檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)均設(she)(she)雙(shuang)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)，以(yi)(yi)(yi)保(bao)(bao)(bao)(bao)(bao)證(zheng)(zheng)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)結果的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)。 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)激(ji)(ji)(ji)(ji)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)脫(tuo)(tuo)落(luo)(luo)(luo)(luo)(luo)酸(suan)(ABA)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)洗(xi)(xi)板(ban)方(fang)(fang)法手工(gong)(gong)(gong)洗(xi)(xi)板(ban)方(fang)(fang)法：吸去（不(bu)(bu)可(ke)(ke)(ke)觸(chu)及(ji)(ji)(ji)(ji)板(ban)壁）或(huo)(huo)甩(shuai)掉(diao)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)內(nei)(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)；在(zai)(zai)(zai)(zai)實(shi)驗(yan)(yan)臺上(shang)(shang)(shang)鋪(pu)墊(dian)幾層吸水紙(zhi)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)朝下用(yong)(yong)(yong)(yong)(yong)力拍幾次(ci)(ci)(ci)(ci)(ci)；將(jiang)(jiang)(jiang)(jiang)推(tui)薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)(di)緩沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)至少0.3ml注(zhu)入(ru)(ru)(ru)(ru)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)，浸(jin)泡(pao)1-2分(fen)(fen)鐘(zhong)(zhong)。根據(ju)需(xu)要(yao)(yao)，重復(fu)此(ci)過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)數(shu)次(ci)(ci)(ci)(ci)(ci)。 自動洗(xi)(xi)板(ban)：如(ru)(ru)果有(you)自動洗(xi)(xi)板(ban)機，應(ying)(ying)(ying)(ying)(ying)(ying)在(zai)(zai)(zai)(zai)熟練使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)再(zai)(zai)用(yong)(yong)(yong)(yong)(yong)到正式(shi)(shi)(shi)實(shi)驗(yan)(yan)過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)。 計(ji)(ji)(ji)(ji)算   以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)橫(heng)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)為(wei)(wei)(wei)縱(zong)(zong)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普(pu)通坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)(zai)半對數(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)(shang)繪(hui)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)，根據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)查出(chu)相(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)；再(zai)(zai)乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)；或(huo)(huo)用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)與OD值(zhi)計(ji)(ji)(ji)(ji)算出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)(xian)(xian)回(hui)(hui)歸(gui)方(fang)(fang)程(cheng)(cheng)(cheng)(cheng)式(shi)(shi)(shi)，將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代(dai)入(ru)(ru)(ru)(ru)方(fang)(fang)程(cheng)(cheng)(cheng)(cheng)式(shi)(shi)(shi)，計(ji)(ji)(ji)(ji)算出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)，再(zai)(zai)乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)，即(ji)(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。 注(zhu)意(yi)事(shi)(shi)項1.當混合蛋(dan)白(bai)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)量輕(qing)(qing)緩，避(bi)(bi)免(mian)(mian)起(qi)泡(pao)。2.洗(xi)(xi)滌(di)(di)過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)非(fei)常重要(yao)(yao)，不(bu)(bu)充(chong)分(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)(di)易(yi)造成假陽(yang)性(xing)(xing)。3.一(yi)次(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)間(jian)**控制(zhi)在(zai)(zai)(zai)(zai)5分(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)(nei)，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)量多(duo)(duo)，推(tui)薦使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4.請(qing)(qing)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi)(shi)(shi)(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)，做復(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。5.如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中(zhong)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含(han)量過(guo)(guo)(guo)高，請(qing)(qing)先(xian)(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)再(zai)(zai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)，計(ji)(ji)(ji)(ji)算時(shi)(shi)(shi)(shi)(shi)(shi)請(qing)(qing)乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)。6.在(zai)(zai)(zai)(zai)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)(qing)以(yi)(yi)(yi)相(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)，不(bu)(bu)能混淆。7.底物(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)避(bi)(bi)光(guang)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。 8.不(bu)(bu)要(yao)(yao)用(yong)(yong)(yong)(yong)(yong)其(qi)(qi)它(ta)生(sheng)(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)替換(huan)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)。 注(zhu)意(yi)事(shi)(shi)項：收集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)前(qian)(qian)(qian)(qian)必(bi)須清(qing)楚要(yao)(yao)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成份是(shi)否(fou)足夠穩定(ding)。對收集后(hou)(hou)(hou)當天進(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)，儲存(cun)(cun)(cun)在(zai)(zai)(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)有(you)特殊原因需(xu)要(yao)(yao)周期收集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)， 將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)及(ji)(ji)(ji)(ji)時(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)裝后(hou)(hou)(hou)放(fang)(fang)在(zai)(zai)(zai)(zai)-20 ℃或(huo)(huo)-70 ℃條(tiao)件(jian)下保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。避(bi)(bi)免(mian)(mian)反(fan)復(fu)凍融。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)48 小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)1 個月。-70度(du)(du)(du)(du)(du)(du)(du)可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)6 個月。部分(fen)(fen)激(ji)(ji)(ji)(ji)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)類(lei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)需(xu)添(tian)加(jia)(jia)(jia)(jia)(jia)抑肽酶(mei)(mei)(mei)(mei)(mei)。計(ji)(ji)(ji)(ji)算：以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)橫(heng)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)為(wei)(wei)(wei)縱(zong)(zong)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)(shang)繪(hui)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)，根據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)查出(chu)相(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)；再(zai)(zai)乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)；或(huo)(huo)用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)與OD值(zhi)計(ji)(ji)(ji)(ji)算出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)(xian)(xian)回(hui)(hui)歸(gui)方(fang)(fang)程(cheng)(cheng)(cheng)(cheng)式(shi)(shi)(shi)，將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代(dai)入(ru)(ru)(ru)(ru)方(fang)(fang)程(cheng)(cheng)(cheng)(cheng)式(shi)(shi)(shi)，計(ji)(ji)(ji)(ji)算出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)，再(zai)(zai)乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)，即(ji)(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)激(ji)(ji)(ji)(ji)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)脫(tuo)(tuo)落(luo)(luo)(luo)(luo)(luo)酸(suan)(ABA)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)性(xing)(xing)能1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)線(xian)(xian)(xian)性(xing)(xing)回(hui)(hui)歸(gui)與預期濃(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)相(xiang)關系數(shu)R值(zhi)為(wei)(wei)(wei)0.92以(yi)(yi)(yi)上(shang)(shang)(shang)。2.批內(nei)(nei)(nei)(nei)與批見(jian)應(ying)(ying)(ying)(ying)(ying)(ying)分(fen)(fen)別(bie)小(xiao)于9%和(he)15% 植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)激(ji)(ji)(ji)(ji)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)脫(tuo)(tuo)落(luo)(luo)(luo)(luo)(luo)酸(suan)(ABA)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)