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 小鼠(shu)胰(yi)島(dao)素(su)(su)(su)(su)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)生(sheng)(sheng)長(chang)因子(zi)(zi) 1（IGF-1）實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)原理   試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)雙(shuang)抗(kang)(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)一步夾心法酶(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)疫(yi)吸附試(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)（ELISA）。往預(yu)(yu)先(xian)包(bao)被(bei)(bei)脂(zhi)肪(fang)酸(suan)(suan)結(jie)合蛋白(bai)1(FABP1)捕獲抗(kang)(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包(bao)被(bei)(bei)微孔(kong)(kong)(kong)中(zhong)(zhong)，依(yi)次(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)，經過(guo)(guo)(guo)(guo)溫(wen)育并徹底(di)(di)(di)(di)(di)洗(xi)(xi)滌。用(yong)(yong)(yong)(yong)(yong)(yong)底(di)(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯(xian)色(se)，TMB在(zai)(zai)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)下(xia)轉化(hua)成(cheng)藍(lan)色(se)，并在(zai)(zai)酸(suan)(suan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)下(xia)轉化(hua)成(cheng)＊終的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)色(se)。顏(yan)色(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂(zhi)肪(fang)酸(suan)(suan)結(jie)合蛋白(bai)1(FABP1)呈正(zheng)相關。用(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)在(zai)(zai)450nm 波長(chang)下(xia)測(ce)(ce)(ce)(ce)(ce)定吸光度(du)(du)(du)(du)(du)(du)(du)（OD 值），計算(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。保(bao)(bao)(bao)存(cun)(cun)條(tiao)(tiao)件及(ji)有(you)(you)(you)(you)效(xiao)期本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)體(ti)(ti)(ti)(ti)外定量(liang)(liang)(liang)(liang)(liang)(liang)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)血(xue)(xue)清(qing)、血(xue)(xue)漿、組織勻(yun)(yun)漿及(ji)相關液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)脂(zhi)肪(fang)酸(suan)(suan)結(jie)合蛋白(bai)1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)(liang)(liang)(liang)(liang)。有(you)(you)(you)(you)效(xiao)期：6個月(yue)(yue)(yue)保(bao)(bao)(bao)存(cun)(cun)條(tiao)(tiao)件：2-8℃小鼠(shu)胰(yi)島(dao)素(su)(su)(su)(su)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)生(sheng)(sheng)長(chang)因子(zi)(zi) 1（IGF-1）一、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集(ji)及(ji)保(bao)(bao)(bao)存(cun)(cun) 1.血(xue)(xue)清(qing)：全血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)請(qing)(qing)(qing)(qing)(qing)于(yu)(yu)(yu)室溫(wen)放(fang)(fang)(fang)(fang)置2小時(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)4℃過(guo)(guo)(guo)(guo)一晚后(hou)(hou)(hou)(hou)于(yu)(yu)(yu)1000 x g離(li)心20分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，取(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)即(ji)可(ke)(ke)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)放(fang)(fang)(fang)(fang)于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)存(cun)(cun)，但應(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)(mian)反(fan)復(fu)凍融(rong)。 2.血(xue)(xue)漿：可(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)肝素(su)(su)(su)(su)作(zuo)(zuo)為(wei)(wei)(wei)抗(kang)(kang)(kang)(kang)(kang)(kang)凝劑(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)采(cai)集(ji)后(hou)(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)于(yu)(yu)(yu)2 - 8° C 1000 x g離(li)心15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)放(fang)(fang)(fang)(fang)于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)存(cun)(cun)，但應(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)(mian)反(fan)復(fu)凍融(rong)。 3.細胞培(pei)養(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)(shang)清(qing)或(huo)(huo)(huo)其(qi)(qi)它(ta)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)：1000 x g離(li)心20分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，取(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)即(ji)可(ke)(ke)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)放(fang)(fang)(fang)(fang)于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)存(cun)(cun)，但應(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)(mian)反(fan)復(fu)凍融(rong)。 注：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)溶(rong)血(xue)(xue)會影響檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)(guo)(guo)，因此(ci)(ci)(ci)(ci)(ci)溶(rong)血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)不(bu)(bu)(bu)(bu)宜進行此(ci)(ci)(ci)(ci)(ci)項(xiang)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)。二、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原則(ze)(ze)   首先(xian)通過(guo)(guo)(guo)(guo)文獻(xian)檢(jian)(jian)(jian)索(suo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)(fang)式(shi)了(le)(le)解待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含量(liang)(liang)(liang)(liang)(liang)(liang)，確(que)定適當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)。只有(you)(you)(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍(wei)內(nei)(nei)，檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果(guo)(guo)(guo)才(cai)是(shi)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)，應(ying)(ying)(ying)做(zuo)(zuo)(zuo)好(hao)詳(xiang)細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)錄。計算(suan)(suan)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)了(le)(le)“N”倍(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)應(ying)(ying)(ying)再(zai)(zai)(zai)(zai)乘(cheng)以(yi)(yi)(yi)(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原則(ze)(ze)：2瓶，每(mei)(mei)瓶臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)至1ml，蓋好(hao)后(hou)(hou)(hou)(hou)靜置10分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)上(shang)(shang)(shang)(shang)，然后(hou)(hou)(hou)(hou)反(fan)復(fu)顛倒/搓動(dong)(dong)以(yi)(yi)(yi)(yi)助溶(rong)解，其(qi)(qi)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)300 pg/ml，做(zuo)(zuo)(zuo)系列(lie)倍(bei)(bei)比稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)直接作(zuo)(zuo)為(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)0 pg/ml，臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)配(pei)(pei)制(zhi)。 如(ru)配(pei)(pei)制(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)：取(qu)(qu)0.5ml（不(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)少于(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)(shang)述(shu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)含0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)中(zhong)(zhong)，混勻(yun)(yun)即(ji)可(ke)(ke)，其(qi)(qi)余濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)以(yi)(yi)(yi)(yi)此(ci)(ci)(ci)(ci)(ci)類(lei)推。2.生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原則(ze)(ze)： 臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)(gen)據(ju)(ju)預(yu)(yu)先(xian)計算(suan)(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)所(suo)(suo)需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)(liang)(liang)(liang)配(pei)(pei)制(zhi)（每(mei)(mei)孔(kong)(kong)(kong)100μl），實(shi)(shi)(shi)際配(pei)(pei)制(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)多(duo)(duo)配(pei)(pei)制(zhi)0.1-0.2ml。如(ru)10μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例配(pei)(pei)制(zhi)，輕(qing)輕(qing)混勻(yun)(yun)，在(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一小時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)制(zhi)。3.辣(la)根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原則(ze)(ze)： 臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)辣(la)根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)(gen)據(ju)(ju)預(yu)(yu)先(xian)計算(suan)(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)所(suo)(suo)需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)(liang)(liang)(liang)配(pei)(pei)制(zhi)（每(mei)(mei)孔(kong)(kong)(kong)100μl），實(shi)(shi)(shi)際配(pei)(pei)制(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)多(duo)(duo)配(pei)(pei)制(zhi)0.1-0.2ml。如(ru)10μl辣(la)根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl辣(la)根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例配(pei)(pei)制(zhi)，輕(qing)輕(qing)混勻(yun)(yun)，在(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一小時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)制(zhi)。 操作(zuo)(zuo)步驟(zou) 實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)開始前(qian)(qian)(qian)，請(qing)(qing)(qing)(qing)(qing)提前(qian)(qian)(qian)配(pei)(pei)置好(hao)所(suo)(suo)有(you)(you)(you)(you)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)或(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)，均需(xu)(xu)混勻(yun)(yun)，混勻(yun)(yun)時(shi)(shi)(shi)(shi)(shi)(shi)盡(jin)(jin)量(liang)(liang)(liang)(liang)(liang)(liang)避(bi)(bi)免(mian)(mian)(mian)起泡。每(mei)(mei)次(ci)(ci)(ci)(ci)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)該做(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)。如(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)過(guo)(guo)(guo)(guo)高時(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)進行稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)使(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)符合試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)范圍(wei)。 小鼠(shu)胰(yi)島(dao)素(su)(su)(su)(su)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)生(sheng)(sheng)長(chang)因子(zi)(zi) 1（IGF-1）三、操作(zuo)(zuo)步驟(zou)1.加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)設空白(bai)孔(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)、待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)。空白(bai)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)或(huo)(huo)(huo)待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)100μl，注意不(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)有(you)(you)(you)(you)氣泡，加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)(yu)(yu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)(kong)(kong)底(di)(di)(di)(di)(di)部，盡(jin)(jin)量(liang)(liang)(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)觸(chu)及(ji)孔(kong)(kong)(kong)壁，輕(qing)輕(qing)晃動(dong)(dong)混勻(yun)(yun)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)(shang)蓋或(huo)(huo)(huo)覆膜，37℃反(fan)應(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)保(bao)(bao)(bao)證(zheng)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)結(jie)果(guo)(guo)(guo)有(you)(you)(you)(you)效(xiao)性，每(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)請(qing)(qing)(qing)(qing)(qing)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)新(xin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)溶(rong)液(ye)(ye)(ye)(ye)。2.棄(qi)(qi)去(qu)液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干，不(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)滌。每(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye) 100μl（取(qu)(qu)1μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例配(pei)(pei)制(zhi)，輕(qing)輕(qing)混勻(yun)(yun)，在(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一小時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)制(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。 3.溫(wen)育60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)，棄(qi)(qi)去(qu)孔(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干，洗(xi)(xi)板(ban)(ban)3次(ci)(ci)(ci)(ci)，每(mei)(mei)次(ci)(ci)(ci)(ci)浸(jin)泡1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)孔(kong)(kong)(kong)，甩(shuai)(shuai)干。 4.每(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)辣(la)根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)（同生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。 5.溫(wen)育60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)，棄(qi)(qi)去(qu)孔(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干，洗(xi)(xi)板(ban)(ban)5次(ci)(ci)(ci)(ci)，每(mei)(mei)次(ci)(ci)(ci)(ci)浸(jin)泡1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)孔(kong)(kong)(kong)，甩(shuai)(shuai)干。 6.依(yi)序每(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)90μl，37℃避(bi)(bi)光顯(xian)色(se)（30分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)，此(ci)(ci)(ci)(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)肉眼可(ke)(ke)見(jian)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)3-4孔(kong)(kong)(kong)有(you)(you)(you)(you)明(ming)顯(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯(ti)度(du)(du)(du)(du)(du)(du)(du)藍(lan)色(se)，后(hou)(hou)(hou)(hou)3-4孔(kong)(kong)(kong)梯(ti)度(du)(du)(du)(du)(du)(du)(du)不(bu)(bu)(bu)(bu)明(ming)顯(xian)，即(ji)可(ke)(ke)終止）。 7.依(yi)序每(mei)(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終止溶(rong)液(ye)(ye)(ye)(ye)50μl，終止反(fan)應(ying)(ying)(ying)（此(ci)(ci)(ci)(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)藍(lan)色(se)立轉黃(huang)色(se)）。終止液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)順(shun)序應(ying)(ying)(ying)盡(jin)(jin)量(liang)(liang)(liang)(liang)(liang)(liang)與底(di)(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)順(shun)序相同。為(wei)(wei)(wei)了(le)(le)保(bao)(bao)(bao)證(zheng)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)結(jie)果(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性，底(di)(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)應(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)間到(dao)(dao)(dao)后(hou)(hou)(hou)(hou)應(ying)(ying)(ying)盡(jin)(jin)快加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)終止液(ye)(ye)(ye)(ye)。 8.用(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)聯(lian)儀(yi)在(zai)(zai)450nm波長(chang)依(yi)序測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)(liang)(liang)(liang)各(ge)(ge)孔(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光密度(du)(du)(du)(du)(du)(du)(du)（OD值）。 在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終止液(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)內(nei)(nei)進行檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)。 四(si)、試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)小技巧1. 用(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)初次(ci)(ci)(ci)(ci)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)時(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)將(jiang)(jiang)各(ge)(ge)種(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)管(guan)離(li)心數(shu)(shu)(shu)(shu)分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)集(ji)中(zhong)(zhong)到(dao)(dao)(dao)管(guan)底(di)(di)(di)(di)(di)。2. 每(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)留一孔(kong)(kong)(kong)作(zuo)(zuo)為(wei)(wei)(wei)空白(bai)調零(ling)(ling)孔(kong)(kong)(kong)，該孔(kong)(kong)(kong)不(bu)(bu)(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)任何試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，只是(shi)**加(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)及(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)(liang)(liang)(liang)時(shi)(shi)(shi)(shi)(shi)(shi)先(xian)用(yong)(yong)(yong)(yong)(yong)(yong)此(ci)(ci)(ci)(ci)(ci)孔(kong)(kong)(kong)調OD值至零(ling)(ling)。 3. 為(wei)(wei)(wei)防止樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)時(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)反(fan)應(ying)(ying)(ying)板(ban)(ban)放(fang)(fang)(fang)(fang)于(yu)(yu)(yu)鋪(pu)有(you)(you)(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)(he)內(nei)(nei)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)(shang)蓋或(huo)(huo)(huo)覆膜。 4. 未(wei)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)或(huo)(huo)(huo)者(zhe)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，請(qing)(qing)(qing)(qing)(qing)于(yu)(yu)(yu)2-8℃保(bao)(bao)(bao)存(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)、辣(la)根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)請(qing)(qing)(qing)(qing)(qing)依(yi)據(ju)(ju)所(suo)(suo)需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)(liang)(liang)(liang)配(pei)(pei)置使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)(qing)(qing)(qing)勿重復(fu)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)或(huo)(huo)(huo)、辣(la)根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)。 5. 建議檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)均設雙(shuang)孔(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)定，以(yi)(yi)(yi)(yi)保(bao)(bao)(bao)證(zheng)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性。 洗(xi)(xi)板(ban)(ban)方(fang)(fang)(fang)法 手工(gong)洗(xi)(xi)板(ban)(ban)方(fang)(fang)(fang)法：吸去(qu)（不(bu)(bu)(bu)(bu)可(ke)(ke)觸(chu)及(ji)板(ban)(ban)壁）或(huo)(huo)(huo)甩(shuai)(shuai)掉酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)內(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)；在(zai)(zai)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)臺上(shang)(shang)(shang)(shang)鋪(pu)墊幾(ji)層吸水紙(zhi)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)朝下(xia)用(yong)(yong)(yong)(yong)(yong)(yong)力拍幾(ji)次(ci)(ci)(ci)(ci)；將(jiang)(jiang)推薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌緩沖液(ye)(ye)(ye)(ye)至少0.3ml注入(ru)(ru)孔(kong)(kong)(kong)內(nei)(nei)，浸(jin)泡1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。根(gen)(gen)據(ju)(ju)需(xu)(xu)要(yao)(yao)(yao)(yao)，重復(fu)此(ci)(ci)(ci)(ci)(ci)過(guo)(guo)(guo)(guo)程(cheng)數(shu)(shu)(shu)(shu)次(ci)(ci)(ci)(ci)。 自動(dong)(dong)洗(xi)(xi)板(ban)(ban)：如(ru)果(guo)(guo)(guo)有(you)(you)(you)(you)自動(dong)(dong)洗(xi)(xi)板(ban)(ban)機，應(ying)(ying)(ying)在(zai)(zai)熟練使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)(hou)再(zai)(zai)(zai)(zai)用(yong)(yong)(yong)(yong)(yong)(yong)到(dao)(dao)(dao)正(zheng)式(shi)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)過(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)。 計算(suan)(suan) 以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值為(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普(pu)通坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)半對數(shu)(shu)(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)(shang)(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，根(gen)(gen)據(ju)(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)查出(chu)相應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)；再(zai)(zai)(zai)(zai)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)與OD值計算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)回歸(gui)(gui)方(fang)(fang)(fang)程(cheng)式(shi)，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值代入(ru)(ru)方(fang)(fang)(fang)程(cheng)式(shi)，計算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)，再(zai)(zai)(zai)(zai)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)，即(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。 五、技術小提示(shi)1.當(dang)混合蛋白(bai)溶(rong)液(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)盡(jin)(jin)量(liang)(liang)(liang)(liang)(liang)(liang)輕(qing)緩，避(bi)(bi)免(mian)(mian)(mian)起泡。2. 洗(xi)(xi)滌過(guo)(guo)(guo)(guo)程(cheng)非常(chang)重要(yao)(yao)(yao)(yao)，不(bu)(bu)(bu)(bu)充分(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌易(yi)造成(cheng)假陽性。 3. 一次(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)間控制(zhi)在(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)數(shu)(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)多(duo)(duo)，推薦使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。 4. 請(qing)(qing)(qing)(qing)(qing)每(mei)(mei)次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)定的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)(shi)(shi)(shi)做(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，做(zuo)(zuo)(zuo)復(fu)孔(kong)(kong)(kong)。 5. 如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)待測(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)(liang)(liang)(liang)(liang)(liang)過(guo)(guo)(guo)(guo)高，請(qing)(qing)(qing)(qing)(qing)先(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)再(zai)(zai)(zai)(zai)測(ce)(ce)(ce)(ce)(ce)定，計算(suan)(suan)時(shi)(shi)(shi)(shi)(shi)(shi)請(qing)(qing)(qing)(qing)(qing)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)。 6. 在(zai)(zai)配(pei)(pei)制(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)溶(rong)液(ye)(ye)(ye)(ye)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)(qing)(qing)(qing)(qing)以(yi)(yi)(yi)(yi)相應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)配(pei)(pei)制(zhi)，不(bu)(bu)(bu)(bu)能混淆。 7. 底(di)(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)(qing)(qing)(qing)避(bi)(bi)光保(bao)(bao)(bao)存(cun)(cun)。 8. 不(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)用(yong)(yong)(yong)(yong)(yong)(yong)其(qi)(qi)它(ta)生(sheng)(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)替換試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)。 六(liu)、注意事項(xiang)1.收集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)前(qian)(qian)(qian)必須清(qing)楚要(yao)(yao)(yao)(yao)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)份是(shi)否足夠穩定。對收集(ji)后(hou)(hou)(hou)(hou)當(dang)天進行檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)，儲存(cun)(cun)在(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)有(you)(you)(you)(you)特殊原因需(xu)(xu)要(yao)(yao)(yao)(yao)周期收集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)， 將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)及(ji)時(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)(fen)裝后(hou)(hou)(hou)(hou)放(fang)(fang)(fang)(fang)在(zai)(zai)-20 ℃或(huo)(huo)(huo)-70 ℃條(tiao)(tiao)件下(xia)保(bao)(bao)(bao)存(cun)(cun)。避(bi)(bi)免(mian)(mian)(mian)反(fan)復(fu)凍融(rong)。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)保(bao)(bao)(bao)存(cun)(cun)48 小時(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)保(bao)(bao)(bao)存(cun)(cun)1 個月(yue)(yue)(yue)。-70度(du)(du)(du)(du)(du)(du)(du)可(ke)(ke)保(bao)(bao)(bao)存(cun)(cun)6 個月(yue)(yue)(yue)。3.部分(fen)(fen)(fen)(fen)(fen)(fen)激素(su)(su)(su)(su)類(lei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)添加(jia)(jia)(jia)(jia)(jia)(jia)(jia)抑肽(tai)酶(mei)(mei)(mei)(mei)。 4.計算(suan)(suan)方(fang)(fang)(fang)法：以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值為(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)(shang)(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，根(gen)(gen)據(ju)(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)查出(chu)相應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)；再(zai)(zai)(zai)(zai)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)與OD值計算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)回歸(gui)(gui)方(fang)(fang)(fang)程(cheng)式(shi)，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值代入(ru)(ru)方(fang)(fang)(fang)程(cheng)式(shi)，計算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)，再(zai)(zai)(zai)(zai)乘(cheng)以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)(shu)，即(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。 七、試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)性能1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)線(xian)(xian)(xian)性回歸(gui)(gui)與預(yu)(yu)期濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)相關系數(shu)(shu)(shu)(shu)R值為(wei)(wei)(wei)0.92以(yi)(yi)(yi)(yi)上(shang)(shang)(shang)(shang)。2.批(pi)內(nei)(nei)與批(pi)見(jian)應(ying)(ying)(ying)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)小于(yu)(yu)(yu)9%和(he)(he)(he)15% 八、保(bao)(bao)(bao)存(cun)(cun)條(tiao)(tiao)件及(ji)有(you)(you)(you)(you)效(xiao)期1.試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)保(bao)(bao)(bao)存(cun)(cun)：2-8℃。 2．有(you)(you)(you)(you)效(xiao)期：6個月(yue)(yue)(yue)

 小(xiao)(xiao)鼠神(shen)(shen)經(jing)(jing)膠質纖維(wei)酸(suan)性(xing)(xing)蛋(dan)(dan)白(bai)（GFAP）實(shi)(shi)(shi)驗(yan)(yan)(yan)原(yuan)理(li)   試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)采用(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙(shuang)抗(kang)體(ti)一(yi)步夾心法(fa)(fa)酶(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)疫吸(xi)附試(shi)(shi)(shi)驗(yan)(yan)(yan)（ELISA）。往預(yu)(yu)先(xian)包被脂肪(fang)酸(suan)結(jie)合蛋(dan)(dan)白(bai)1(FABP1)捕獲抗(kang)體(ti)的(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，依(yi)次(ci)(ci)加(jia)(jia)(jia)入(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)測(ce)抗(kang)體(ti)，經(jing)(jing)過(guo)(guo)(guo)(guo)溫育并徹底洗(xi)滌。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)底物(wu)(wu)(wu)(wu)(wu)TMB顯(xian)色，TMB在(zai)(zai)(zai)過(guo)(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)(hua)(hua)下(xia)(xia)(xia)轉化(hua)(hua)(hua)(hua)成藍色，并在(zai)(zai)(zai)酸(suan)的(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)下(xia)(xia)(xia)轉化(hua)(hua)(hua)(hua)成＊終(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)色。顏色的(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)脂肪(fang)酸(suan)結(jie)合蛋(dan)(dan)白(bai)1(FABP1)呈正相關(guan)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)在(zai)(zai)(zai)450nm 波長下(xia)(xia)(xia)測(ce)定(ding)(ding)吸(xi)光度(du)(du)(du)(du)(du)(du)(du)(du)（OD 值(zhi)(zhi)），計(ji)(ji)算(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)。保(bao)存(cun)(cun)(cun)(cun)(cun)條(tiao)件及有(you)(you)效期(qi)(qi)本(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)體(ti)外定(ding)(ding)量(liang)(liang)(liang)檢(jian)(jian)測(ce)血(xue)(xue)清(qing)、血(xue)(xue)漿(jiang)、組織勻(yun)漿(jiang)及相關(guan)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)脂肪(fang)酸(suan)結(jie)合蛋(dan)(dan)白(bai)1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)含(han)(han)量(liang)(liang)(liang)。有(you)(you)效期(qi)(qi)：6個(ge)月(yue)(yue)保(bao)存(cun)(cun)(cun)(cun)(cun)條(tiao)件：2-8℃小(xiao)(xiao)鼠神(shen)(shen)經(jing)(jing)膠質纖維(wei)酸(suan)性(xing)(xing)蛋(dan)(dan)白(bai)（GFAP）一(yi)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)采集及保(bao)存(cun)(cun)(cun)(cun)(cun) 1.血(xue)(xue)清(qing)：全(quan)血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)請(qing)(qing)(qing)于(yu)室溫放置(zhi)2小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)4℃過(guo)(guo)(guo)(guo)一(yi)晚(wan)后(hou)(hou)(hou)于(yu)1000 x g離(li)心20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，取上(shang)(shang)(shang)(shang)清(qing)即(ji)可(ke)(ke)檢(jian)(jian)測(ce)，或(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)放于(yu)-20℃或(huo)(huo)-80℃保(bao)存(cun)(cun)(cun)(cun)(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)免(mian)反(fan)(fan)復(fu)(fu)(fu)凍(dong)融。 2.血(xue)(xue)漿(jiang)：可(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)肝(gan)素(su)作(zuo)(zuo)為(wei)(wei)(wei)抗(kang)凝(ning)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)采集后(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)于(yu)2 - 8° C 1000 x g離(li)心15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)放于(yu)-20℃或(huo)(huo)-80℃保(bao)存(cun)(cun)(cun)(cun)(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)免(mian)反(fan)(fan)復(fu)(fu)(fu)凍(dong)融。 3.細胞培(pei)養物(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)(shang)清(qing)或(huo)(huo)其它生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)：1000 x g離(li)心20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，取上(shang)(shang)(shang)(shang)清(qing)即(ji)可(ke)(ke)檢(jian)(jian)測(ce)，或(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)放于(yu)-20℃或(huo)(huo)-80℃保(bao)存(cun)(cun)(cun)(cun)(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)免(mian)反(fan)(fan)復(fu)(fu)(fu)凍(dong)融。 注(zhu)(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)溶(rong)(rong)血(xue)(xue)會影響檢(jian)(jian)測(ce)結(jie)果(guo)，因此(ci)溶(rong)(rong)血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)不(bu)宜(yi)進(jin)行此(ci)項(xiang)(xiang)檢(jian)(jian)測(ce)。二、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)原(yuan)則(ze)   首先(xian)通過(guo)(guo)(guo)(guo)文獻檢(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)方式(shi)(shi)了解待(dai)測(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)大致含(han)(han)量(liang)(liang)(liang)，確(que)定(ding)(ding)適當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)(shu)(shu)。只有(you)(you)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)范圍(wei)內(nei)，檢(jian)(jian)測(ce)的(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果(guo)才是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)程中(zhong)，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)做(zuo)(zuo)好(hao)(hao)詳細的(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)錄。計(ji)(ji)算(suan)(suan)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)了“N”倍(bei)(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)再乘(cheng)(cheng)以(yi)(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)原(yuan)則(ze)：2瓶(ping)，每瓶(ping)臨(lin)(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)以(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)至1ml，蓋(gai)(gai)好(hao)(hao)后(hou)(hou)(hou)靜置(zhi)10分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)以(yi)(yi)上(shang)(shang)(shang)(shang)，然后(hou)(hou)(hou)反(fan)(fan)復(fu)(fu)(fu)顛倒/搓動以(yi)(yi)助(zhu)溶(rong)(rong)解，其濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)300 pg/ml，做(zuo)(zuo)系(xi)列倍(bei)(bei)(bei)比稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)后(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)別(bie)(bie)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)直接作(zuo)(zuo)為(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)0 pg/ml，臨(lin)(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。 如配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)：取0.5ml（不(bu)要(yao)(yao)(yao)少于(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)(shang)述(shu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)入(ru)(ru)(ru)含(han)(han)0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)中(zhong)，混勻(yun)即(ji)可(ke)(ke)，其余濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)以(yi)(yi)此(ci)類推。2.生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)原(yuan)則(ze)： 臨(lin)(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)以(yi)(yi)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)前(qian)根(gen)(gen)(gen)(gen)據(ju)預(yu)(yu)先(xian)計(ji)(ji)算(suan)(suan)好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)每次(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)所(suo)(suo)需的(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)（每孔(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)際配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)多(duo)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如10μl生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)加(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)比例配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混勻(yun)，在(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)一(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。3.辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)素(su)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)原(yuan)則(ze)： 臨(lin)(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)以(yi)(yi)辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)素(su)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)前(qian)根(gen)(gen)(gen)(gen)據(ju)預(yu)(yu)先(xian)計(ji)(ji)算(suan)(suan)好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)每次(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)所(suo)(suo)需的(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)（每孔(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)際配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)多(duo)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如10μl辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)素(su)加(jia)(jia)(jia)990μl辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)素(su)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)比例配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混勻(yun)，在(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)一(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。 操作(zuo)(zuo)步驟 實(shi)(shi)(shi)驗(yan)(yan)(yan)開始前(qian)，請(qing)(qing)(qing)提前(qian)配(pei)(pei)(pei)(pei)置(zhi)好(hao)(hao)所(suo)(suo)有(you)(you)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)或(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)，均需混勻(yun)，混勻(yun)時(shi)(shi)(shi)(shi)(shi)(shi)盡量(liang)(liang)(liang)避(bi)免(mian)起泡(pao)。每次(ci)(ci)檢(jian)(jian)測(ce)都應(ying)(ying)(ying)(ying)(ying)(ying)(ying)該做(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)。如樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)過(guo)(guo)(guo)(guo)高時(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)進(jin)行稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)，以(yi)(yi)使(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)符合試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)測(ce)范圍(wei)。 小(xiao)(xiao)鼠神(shen)(shen)經(jing)(jing)膠質纖維(wei)酸(suan)性(xing)(xing)蛋(dan)(dan)白(bai)（GFAP）三、操作(zuo)(zuo)步驟1.加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)別(bie)(bie)設(she)(she)空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)(kong)、待(dai)測(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)。空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)待(dai)測(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)(zhu)意(yi)(yi)不(bu)要(yao)(yao)(yao)有(you)(you)氣泡(pao)，加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)于(yu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)底部，盡量(liang)(liang)(liang)不(bu)觸及孔(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)(qing)輕(qing)(qing)晃動混勻(yun)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)加(jia)(jia)(jia)上(shang)(shang)(shang)(shang)蓋(gai)(gai)或(huo)(huo)覆膜，37℃反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)保(bao)證實(shi)(shi)(shi)驗(yan)(yan)(yan)結(jie)果(guo)有(you)(you)效性(xing)(xing)，每次(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)請(qing)(qing)(qing)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。2.棄(qi)去液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，甩(shuai)干(gan)(gan)，不(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)滌。每孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)加(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)比例配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混勻(yun)，在(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)一(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。 3.溫育60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)，棄(qi)去孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，甩(shuai)干(gan)(gan)，洗(xi)板(ban)(ban)(ban)(ban)3次(ci)(ci)，每次(ci)(ci)浸(jin)泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，350μl/每孔(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)干(gan)(gan)。 4.每孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)素(su)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。 5.溫育60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)，棄(qi)去孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，甩(shuai)干(gan)(gan)，洗(xi)板(ban)(ban)(ban)(ban)5次(ci)(ci)，每次(ci)(ci)浸(jin)泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，350μl/每孔(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)干(gan)(gan)。 6.依(yi)序(xu)每孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)底物(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)光顯(xian)色（30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)，此(ci)時(shi)(shi)(shi)(shi)(shi)(shi)肉眼可(ke)(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)前(qian)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)有(you)(you)明顯(xian)的(de)(de)(de)(de)(de)(de)(de)(de)梯(ti)度(du)(du)(du)(du)(du)(du)(du)(du)藍色，后(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)梯(ti)度(du)(du)(du)(du)(du)(du)(du)(du)不(bu)明顯(xian)，即(ji)可(ke)(ke)終(zhong)(zhong)止(zhi)）。 7.依(yi)序(xu)每孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)終(zhong)(zhong)止(zhi)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)止(zhi)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)（此(ci)時(shi)(shi)(shi)(shi)(shi)(shi)藍色立轉黃(huang)色）。終(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)入(ru)(ru)(ru)順序(xu)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡量(liang)(liang)(liang)與(yu)底物(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)入(ru)(ru)(ru)順序(xu)相同。為(wei)(wei)(wei)了保(bao)證實(shi)(shi)(shi)驗(yan)(yan)(yan)結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)，底物(wu)(wu)(wu)(wu)(wu)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)間(jian)到(dao)后(hou)(hou)(hou)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡快加(jia)(jia)(jia)入(ru)(ru)(ru)終(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)聯(lian)儀(yi)在(zai)(zai)(zai)450nm波長依(yi)序(xu)測(ce)量(liang)(liang)(liang)各孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)光密(mi)度(du)(du)(du)(du)(du)(du)(du)(du)（OD值(zhi)(zhi)）。 在(zai)(zai)(zai)加(jia)(jia)(jia)終(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)以(yi)(yi)內(nei)進(jin)行檢(jian)(jian)測(ce)。 四、試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)小(xiao)(xiao)技巧1. 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)(zai)初次(ci)(ci)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)時(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)將(jiang)(jiang)(jiang)(jiang)各種試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)管(guan)離(li)心數(shu)(shu)(shu)(shu)分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，以(yi)(yi)便試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)集中(zhong)到(dao)管(guan)底。2. 每次(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)留一(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)為(wei)(wei)(wei)空白(bai)調(diao)(diao)零(ling)孔(kong)(kong)(kong)(kong)(kong)(kong)，該孔(kong)(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)(jia)任何試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)，只是**加(jia)(jia)(jia)底物(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)及2N H2SO4。測(ce)量(liang)(liang)(liang)時(shi)(shi)(shi)(shi)(shi)(shi)先(xian)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)此(ci)孔(kong)(kong)(kong)(kong)(kong)(kong)調(diao)(diao)OD值(zhi)(zhi)至零(ling)。 3. 為(wei)(wei)(wei)防止(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)驗(yan)(yan)(yan)時(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)(jiang)(jiang)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)板(ban)(ban)(ban)(ban)放于(yu)鋪(pu)有(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)密(mi)閉盒(he)(he)(he)(he)(he)內(nei)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)加(jia)(jia)(jia)上(shang)(shang)(shang)(shang)蓋(gai)(gai)或(huo)(huo)覆膜。 4. 未(wei)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)或(huo)(huo)者(zhe)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)，請(qing)(qing)(qing)于(yu)2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)素(su)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)請(qing)(qing)(qing)依(yi)據(ju)所(suo)(suo)需的(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)置(zhi)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)(qing)勿重(zhong)復(fu)(fu)(fu)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)過(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)、辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)素(su)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5. 建議檢(jian)(jian)測(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)均設(she)(she)雙(shuang)孔(kong)(kong)(kong)(kong)(kong)(kong)測(ce)定(ding)(ding)，以(yi)(yi)保(bao)證檢(jian)(jian)測(ce)結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)。 洗(xi)板(ban)(ban)(ban)(ban)方法(fa)(fa) 手工(gong)(gong)洗(xi)板(ban)(ban)(ban)(ban)方法(fa)(fa)：吸(xi)去（不(bu)可(ke)(ke)觸及板(ban)(ban)(ban)(ban)壁）或(huo)(huo)甩(shuai)掉酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)內(nei)的(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)；在(zai)(zai)(zai)實(shi)(shi)(shi)驗(yan)(yan)(yan)臺上(shang)(shang)(shang)(shang)鋪(pu)墊幾層吸(xi)水(shui)紙(zhi)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)朝下(xia)(xia)(xia)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)力(li)拍幾次(ci)(ci)；將(jiang)(jiang)(jiang)(jiang)推薦的(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌緩沖液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)至少0.3ml注(zhu)(zhu)入(ru)(ru)(ru)孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)，浸(jin)泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。根(gen)(gen)(gen)(gen)據(ju)需要(yao)(yao)(yao)，重(zhong)復(fu)(fu)(fu)此(ci)過(guo)(guo)(guo)(guo)程數(shu)(shu)(shu)(shu)次(ci)(ci)。 自動洗(xi)板(ban)(ban)(ban)(ban)：如果(guo)有(you)(you)自動洗(xi)板(ban)(ban)(ban)(ban)機，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)在(zai)(zai)(zai)熟練使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)再用(yong)(yong)(yong)(yong)(yong)(yong)(yong)到(dao)正式(shi)(shi)實(shi)(shi)(shi)驗(yan)(yan)(yan)過(guo)(guo)(guo)(guo)程中(zhong)。 計(ji)(ji)算(suan)(suan) 以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)橫(heng)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對(dui)(dui)(dui)數(shu)(shu)(shu)(shu)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)為(wei)(wei)(wei)縱(zong)(zong)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普(pu)通坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)半對(dui)(dui)(dui)數(shu)(shu)(shu)(shu)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)(shang)(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，根(gen)(gen)(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)查出(chu)相應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)；再乘(cheng)(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)(shu)(shu)；或(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)計(ji)(ji)算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)回歸(gui)方程式(shi)(shi)，將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)代入(ru)(ru)(ru)方程式(shi)(shi)，計(ji)(ji)算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)，再乘(cheng)(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)(shu)(shu)，即(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)。 五、技術小(xiao)(xiao)提示1.當(dang)混合蛋(dan)(dan)白(bai)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡量(liang)(liang)(liang)輕(qing)(qing)緩，避(bi)免(mian)起泡(pao)。2. 洗(xi)滌過(guo)(guo)(guo)(guo)程非常(chang)重(zhong)要(yao)(yao)(yao)，不(bu)充分(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌易造(zao)成假陽性(xing)(xing)。 3. 一(yi)次(ci)(ci)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)間(jian)控制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)在(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)，如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)數(shu)(shu)(shu)(shu)量(liang)(liang)(liang)多(duo)，推薦使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)。 4. 請(qing)(qing)(qing)每次(ci)(ci)測(ce)定(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)(shi)(shi)(shi)做(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，做(zuo)(zuo)復(fu)(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)。 5. 如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)待(dai)測(ce)物(wu)(wu)(wu)(wu)(wu)質含(han)(han)量(liang)(liang)(liang)過(guo)(guo)(guo)(guo)高，請(qing)(qing)(qing)先(xian)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)后(hou)(hou)(hou)再測(ce)定(ding)(ding)，計(ji)(ji)算(suan)(suan)時(shi)(shi)(shi)(shi)(shi)(shi)請(qing)(qing)(qing)乘(cheng)(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)(shu)(shu)。 6. 在(zai)(zai)(zai)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)測(ce)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)(qing)(qing)以(yi)(yi)相應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，不(bu)能混淆。 7. 底物(wu)(wu)(wu)(wu)(wu)請(qing)(qing)(qing)避(bi)光保(bao)存(cun)(cun)(cun)(cun)(cun)。 8. 不(bu)要(yao)(yao)(yao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)其它生(sheng)(sheng)(sheng)(sheng)產廠(chang)家(jia)的(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)替換試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)。 六、注(zhu)(zhu)意(yi)(yi)事項(xiang)(xiang)1.收集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)前(qian)必(bi)須清(qing)楚要(yao)(yao)(yao)檢(jian)(jian)測(ce)的(de)(de)(de)(de)(de)(de)(de)(de)成份是否足夠穩定(ding)(ding)。對(dui)(dui)(dui)收集后(hou)(hou)(hou)當(dang)天進(jin)行檢(jian)(jian)測(ce)的(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)，儲存(cun)(cun)(cun)(cun)(cun)在(zai)(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如有(you)(you)特(te)殊原(yuan)因需要(yao)(yao)(yao)周期(qi)(qi)收集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)， 將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)及時(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)裝(zhuang)后(hou)(hou)(hou)放在(zai)(zai)(zai)-20 ℃或(huo)(huo)-70 ℃條(tiao)件下(xia)(xia)(xia)保(bao)存(cun)(cun)(cun)(cun)(cun)。避(bi)免(mian)反(fan)(fan)復(fu)(fu)(fu)凍(dong)融。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)保(bao)存(cun)(cun)(cun)(cun)(cun)48 小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)保(bao)存(cun)(cun)(cun)(cun)(cun)1 個(ge)月(yue)(yue)。-70度(du)(du)(du)(du)(du)(du)(du)(du)可(ke)(ke)保(bao)存(cun)(cun)(cun)(cun)(cun)6 個(ge)月(yue)(yue)。3.部分(fen)(fen)(fen)(fen)激素(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)需添(tian)加(jia)(jia)(jia)抑(yi)肽酶(mei)(mei)(mei)(mei)(mei)。 4.計(ji)(ji)算(suan)(suan)方法(fa)(fa)：以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)橫(heng)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)為(wei)(wei)(wei)縱(zong)(zong)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)(shang)(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，根(gen)(gen)(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)查出(chu)相應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)；再乘(cheng)(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)(shu)(shu)；或(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)計(ji)(ji)算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)回歸(gui)方程式(shi)(shi)，將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)代入(ru)(ru)(ru)方程式(shi)(shi)，計(ji)(ji)算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)，再乘(cheng)(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)(shu)(shu)，即(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)。 七、試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)性(xing)(xing)能1.樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)(xian)性(xing)(xing)回歸(gui)與(yu)預(yu)(yu)期(qi)(qi)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)(du)相關(guan)系(xi)數(shu)(shu)(shu)(shu)R值(zhi)(zhi)為(wei)(wei)(wei)0.92以(yi)(yi)上(shang)(shang)(shang)(shang)。2.批內(nei)與(yu)批見應(ying)(ying)(ying)(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)別(bie)(bie)小(xiao)(xiao)于(yu)9%和(he)15% 八、保(bao)存(cun)(cun)(cun)(cun)(cun)條(tiao)件及有(you)(you)效期(qi)(qi)1.試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)保(bao)存(cun)(cun)(cun)(cun)(cun)：2-8℃。 2．有(you)(you)效期(qi)(qi)：6個(ge)月(yue)(yue)

 小(xiao)鼠(shu)總(zong)(zong) Tau 蛋(dan)白(bai)(bai)（T-Tau）ELISA 檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)原(yuan)(yuan)理(li)   試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)采用(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙抗(kang)體(ti)(ti)(ti)一步(bu)夾心法酶(mei)(mei)聯免(mian)(mian)疫吸(xi)附試(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)（ELISA）。往預(yu)先包被脂(zhi)肪酸(suan)(suan)結(jie)(jie)(jie)(jie)合(he)蛋(dan)白(bai)(bai)1(FABP1)捕獲抗(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，依(yi)(yi)次(ci)(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)入標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)抗(kang)體(ti)(ti)(ti)，經過(guo)(guo)(guo)(guo)(guo)溫(wen)育并(bing)徹(che)底(di)(di)(di)(di)洗(xi)(xi)滌。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)TMB顯(xian)(xian)色(se)(se)，TMB在(zai)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)(hua)(hua)下(xia)(xia)轉化(hua)(hua)(hua)(hua)成(cheng)藍色(se)(se)，并(bing)在(zai)酸(suan)(suan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)下(xia)(xia)轉化(hua)(hua)(hua)(hua)成(cheng)＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)(se)。顏色(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂(zhi)肪酸(suan)(suan)結(jie)(jie)(jie)(jie)合(he)蛋(dan)白(bai)(bai)1(FABP1)呈正相(xiang)(xiang)關(guan)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)450nm 波長下(xia)(xia)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)吸(xi)光(guang)度(du)(du)(du)(du)（OD 值(zhi)(zhi)），計(ji)(ji)(ji)算(suan)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)。保(bao)(bao)(bao)存(cun)(cun)(cun)條(tiao)(tiao)(tiao)件(jian)及(ji)(ji)有(you)(you)效期(qi)(qi)本(ben)(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于體(ti)(ti)(ti)外定(ding)(ding)量(liang)(liang)(liang)(liang)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)血清(qing)、血漿(jiang)、組織勻(yun)(yun)(yun)漿(jiang)及(ji)(ji)相(xiang)(xiang)關(guan)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)中(zhong)(zhong)(zhong)脂(zhi)肪酸(suan)(suan)結(jie)(jie)(jie)(jie)合(he)蛋(dan)白(bai)(bai)1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)(liang)(liang)。有(you)(you)效期(qi)(qi)：6個(ge)月保(bao)(bao)(bao)存(cun)(cun)(cun)條(tiao)(tiao)(tiao)件(jian)：2-8℃小(xiao)鼠(shu)總(zong)(zong) Tau 蛋(dan)白(bai)(bai)（T-Tau）ELISA 檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)一、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采集(ji)(ji)及(ji)(ji)保(bao)(bao)(bao)存(cun)(cun)(cun) 1.血清(qing)：全血標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)請(qing)(qing)(qing)(qing)于室溫(wen)放(fang)置(zhi)2小(xiao)時(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)(huo)(huo)4℃過(guo)(guo)(guo)(guo)(guo)一晚后(hou)(hou)于1000 x g離(li)(li)心20分(fen)(fen)(fen)鐘(zhong)(zhong)，取上(shang)(shang)清(qing)即(ji)可(ke)(ke)(ke)(ke)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)放(fang)于-20℃或(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)存(cun)(cun)(cun)，但應(ying)(ying)(ying)避(bi)免(mian)(mian)反(fan)復(fu)凍(dong)融。 2.血漿(jiang)：可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)(huo)肝素(su)(su)(su)(su)(su)作(zuo)(zuo)為(wei)(wei)抗(kang)凝(ning)劑(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)采集(ji)(ji)后(hou)(hou)30分(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)(nei)于2 - 8° C 1000 x g離(li)(li)心15分(fen)(fen)(fen)鐘(zhong)(zhong)，或(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)放(fang)于-20℃或(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)存(cun)(cun)(cun)，但應(ying)(ying)(ying)避(bi)免(mian)(mian)反(fan)復(fu)凍(dong)融。 3.細(xi)胞培養物(wu)(wu)(wu)(wu)上(shang)(shang)清(qing)或(huo)(huo)(huo)(huo)(huo)其(qi)(qi)它生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)：1000 x g離(li)(li)心20分(fen)(fen)(fen)鐘(zhong)(zhong)，取上(shang)(shang)清(qing)即(ji)可(ke)(ke)(ke)(ke)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)放(fang)于-20℃或(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)存(cun)(cun)(cun)，但應(ying)(ying)(ying)避(bi)免(mian)(mian)反(fan)復(fu)凍(dong)融。 注：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)溶(rong)(rong)(rong)(rong)血會影響檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)結(jie)(jie)(jie)(jie)果(guo)，因(yin)此(ci)(ci)溶(rong)(rong)(rong)(rong)血標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)不(bu)(bu)(bu)宜(yi)進(jin)行(xing)此(ci)(ci)項檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)。二、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)原(yuan)(yuan)則(ze)   首先通(tong)過(guo)(guo)(guo)(guo)(guo)文獻檢(jian)(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)式(shi)(shi)了(le)(le)(le)解待(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含量(liang)(liang)(liang)(liang)，確定(ding)(ding)適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)。只有(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)至(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍(wei)(wei)內(nei)(nei)(nei)(nei)，檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)(jie)(jie)(jie)果(guo)才是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)(zhong)，應(ying)(ying)(ying)做(zuo)好(hao)(hao)詳(xiang)細(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)錄。計(ji)(ji)(ji)算(suan)濃(nong)(nong)(nong)度(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)了(le)(le)(le)“N”倍(bei)(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)應(ying)(ying)(ying)再(zai)乘以(yi)(yi)(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)原(yuan)(yuan)則(ze)：2瓶，每(mei)(mei)(mei)瓶臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)至(zhi)1ml，蓋好(hao)(hao)后(hou)(hou)靜置(zhi)10分(fen)(fen)(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)上(shang)(shang)，然(ran)后(hou)(hou)反(fan)復(fu)顛倒/搓(cuo)動以(yi)(yi)(yi)助溶(rong)(rong)(rong)(rong)解，其(qi)(qi)濃(nong)(nong)(nong)度(du)(du)(du)(du)為(wei)(wei)300 pg/ml，做(zuo)系列倍(bei)(bei)(bei)比(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)，分(fen)(fen)(fen)別(bie)(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)直接作(zuo)(zuo)為(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)度(du)(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)15分(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)。 如(ru)(ru)配(pei)(pei)(pei)(pei)制(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)：取0.5ml（不(bu)(bu)(bu)要(yao)(yao)少于0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)入含0.5ml樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)中(zhong)(zhong)(zhong)，混(hun)(hun)(hun)勻(yun)(yun)(yun)即(ji)可(ke)(ke)(ke)(ke)，其(qi)(qi)余濃(nong)(nong)(nong)度(du)(du)(du)(du)以(yi)(yi)(yi)此(ci)(ci)類推(tui)。2.生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)原(yuan)(yuan)則(ze)： 臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)前(qian)(qian)根(gen)(gen)(gen)(gen)據(ju)預(yu)先計(ji)(ji)(ji)算(suan)好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)(zong)量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)際配(pei)(pei)(pei)(pei)制(zhi)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)(pei)(pei)制(zhi)0.1-0.2ml。如(ru)(ru)10μl生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)制(zhi)，輕(qing)輕(qing)混(hun)(hun)(hun)勻(yun)(yun)(yun)，在(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一小(xiao)時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)。3.辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)(qin)和素(su)(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)原(yuan)(yuan)則(ze)： 臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)(qin)和素(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)前(qian)(qian)根(gen)(gen)(gen)(gen)據(ju)預(yu)先計(ji)(ji)(ji)算(suan)好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)(zong)量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)際配(pei)(pei)(pei)(pei)制(zhi)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)(pei)(pei)制(zhi)0.1-0.2ml。如(ru)(ru)10μl辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)(qin)和素(su)(su)(su)(su)(su)加(jia)(jia)(jia)(jia)(jia)990μl辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)(qin)和素(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)制(zhi)，輕(qing)輕(qing)混(hun)(hun)(hun)勻(yun)(yun)(yun)，在(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一小(xiao)時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)。 操(cao)作(zuo)(zuo)步(bu)驟 實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)開始(shi)前(qian)(qian)，請(qing)(qing)(qing)(qing)提前(qian)(qian)配(pei)(pei)(pei)(pei)置(zhi)好(hao)(hao)所有(you)(you)試(shi)(shi)(shi)劑(ji)(ji)(ji)，試(shi)(shi)(shi)劑(ji)(ji)(ji)或(huo)(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)，均需混(hun)(hun)(hun)勻(yun)(yun)(yun)，混(hun)(hun)(hun)勻(yun)(yun)(yun)時(shi)(shi)(shi)(shi)(shi)盡量(liang)(liang)(liang)(liang)避(bi)免(mian)(mian)起泡(pao)。每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)(ci)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)該做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)。如(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)過(guo)(guo)(guo)(guo)(guo)高時(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)進(jin)行(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)使(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)符合(he)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)范圍(wei)(wei)。 小(xiao)鼠(shu)總(zong)(zong) Tau 蛋(dan)白(bai)(bai)（T-Tau）ELISA 檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)三(san)、操(cao)作(zuo)(zuo)步(bu)驟1.加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)別(bie)(bie)設(she)空(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)、待(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)。空(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)(huo)待(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)100μl，注意不(bu)(bu)(bu)要(yao)(yao)有(you)(you)氣泡(pao)，加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)于酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)(kong)(kong)(kong)底(di)(di)(di)(di)部，盡量(liang)(liang)(liang)(liang)不(bu)(bu)(bu)觸(chu)(chu)及(ji)(ji)孔(kong)(kong)(kong)(kong)壁，輕(qing)輕(qing)晃動混(hun)(hun)(hun)勻(yun)(yun)(yun)，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)加(jia)(jia)(jia)(jia)(jia)上(shang)(shang)蓋或(huo)(huo)(huo)(huo)(huo)覆膜，37℃反(fan)應(ying)(ying)(ying)120分(fen)(fen)(fen)鐘(zhong)(zhong)。 為(wei)(wei)保(bao)(bao)(bao)證(zheng)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)結(jie)(jie)(jie)(jie)果(guo)有(you)(you)效性(xing)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)請(qing)(qing)(qing)(qing)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)新(xin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。2.棄去液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩干，不(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)滌。每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)工(gong)(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)制(zhi)，輕(qing)輕(qing)混(hun)(hun)(hun)勻(yun)(yun)(yun)，在(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一小(xiao)時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)），37℃,60分(fen)(fen)(fen)鐘(zhong)(zhong)。 3.溫(wen)育60分(fen)(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)，棄去孔(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩干，洗(xi)(xi)板(ban)(ban)3次(ci)(ci)(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)(ci)浸(jin)(jin)泡(pao)1-2分(fen)(fen)(fen)鐘(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)，甩干。 4.每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)(qin)和素(su)(su)(su)(su)(su)工(gong)(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)工(gong)(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)鐘(zhong)(zhong)。 5.溫(wen)育60分(fen)(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)，棄去孔(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩干，洗(xi)(xi)板(ban)(ban)5次(ci)(ci)(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)(ci)浸(jin)(jin)泡(pao)1-2分(fen)(fen)(fen)鐘(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)，甩干。 6.依(yi)(yi)序每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)光(guang)顯(xian)(xian)色(se)(se)（30分(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)(nei)，此(ci)(ci)時(shi)(shi)(shi)(shi)(shi)肉眼(yan)可(ke)(ke)(ke)(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)3-4孔(kong)(kong)(kong)(kong)有(you)(you)明(ming)顯(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)(du)藍色(se)(se)，后(hou)(hou)3-4孔(kong)(kong)(kong)(kong)梯度(du)(du)(du)(du)不(bu)(bu)(bu)明(ming)顯(xian)(xian)，即(ji)可(ke)(ke)(ke)(ke)終(zhong)止(zhi)）。 7.依(yi)(yi)序每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止(zhi)反(fan)應(ying)(ying)(ying)（此(ci)(ci)時(shi)(shi)(shi)(shi)(shi)藍色(se)(se)立轉黃色(se)(se)）。終(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)入順序應(ying)(ying)(ying)盡量(liang)(liang)(liang)(liang)與底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)入順序相(xiang)(xiang)同。為(wei)(wei)了(le)(le)(le)保(bao)(bao)(bao)證(zheng)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)結(jie)(jie)(jie)(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)，底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)反(fan)應(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)間到(dao)后(hou)(hou)應(ying)(ying)(ying)盡快加(jia)(jia)(jia)(jia)(jia)入終(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)聯儀在(zai)450nm波長依(yi)(yi)序測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)(liang)各(ge)孔(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)密度(du)(du)(du)(du)（OD值(zhi)(zhi)）。 在(zai)加(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)15分(fen)(fen)(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)內(nei)(nei)(nei)(nei)進(jin)行(xing)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)。 四(si)、試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)小(xiao)技巧1. 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)初次(ci)(ci)(ci)(ci)(ci)(ci)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)時(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)將(jiang)(jiang)(jiang)各(ge)種試(shi)(shi)(shi)劑(ji)(ji)(ji)管(guan)離(li)(li)心數(shu)分(fen)(fen)(fen)鐘(zhong)(zhong)，以(yi)(yi)(yi)便試(shi)(shi)(shi)劑(ji)(ji)(ji)集(ji)(ji)中(zhong)(zhong)(zhong)到(dao)管(guan)底(di)(di)(di)(di)。2. 每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)留(liu)一孔(kong)(kong)(kong)(kong)作(zuo)(zuo)為(wei)(wei)空(kong)白(bai)(bai)調(diao)零孔(kong)(kong)(kong)(kong)，該孔(kong)(kong)(kong)(kong)不(bu)(bu)(bu)加(jia)(jia)(jia)(jia)(jia)任何試(shi)(shi)(shi)劑(ji)(ji)(ji)，只是**加(jia)(jia)(jia)(jia)(jia)底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)及(ji)(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)(liang)時(shi)(shi)(shi)(shi)(shi)先用(yong)(yong)(yong)(yong)(yong)(yong)(yong)此(ci)(ci)孔(kong)(kong)(kong)(kong)調(diao)OD值(zhi)(zhi)至(zhi)零。 3. 為(wei)(wei)防止(zhi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)時(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)(jiang)反(fan)應(ying)(ying)(ying)板(ban)(ban)放(fang)于鋪(pu)有(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)內(nei)(nei)(nei)(nei)，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)加(jia)(jia)(jia)(jia)(jia)上(shang)(shang)蓋或(huo)(huo)(huo)(huo)(huo)覆膜。 4. 未使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)或(huo)(huo)(huo)(huo)(huo)者試(shi)(shi)(shi)劑(ji)(ji)(ji)，請(qing)(qing)(qing)(qing)于2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)工(gong)(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)(qin)和素(su)(su)(su)(su)(su)工(gong)(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)請(qing)(qing)(qing)(qing)依(yi)(yi)據(ju)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)置(zhi)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)(qing)(qing)勿重復(fu)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)(ti)(ti)工(gong)(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)(huo)、辣(la)(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)(qin)和素(su)(su)(su)(su)(su)工(gong)(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5. 建議檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)均設(she)雙孔(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)，以(yi)(yi)(yi)保(bao)(bao)(bao)證(zheng)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)結(jie)(jie)(jie)(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)。 洗(xi)(xi)板(ban)(ban)方(fang)(fang)法 手工(gong)(gong)(gong)(gong)洗(xi)(xi)板(ban)(ban)方(fang)(fang)法：吸(xi)去（不(bu)(bu)(bu)可(ke)(ke)(ke)(ke)觸(chu)(chu)及(ji)(ji)板(ban)(ban)壁）或(huo)(huo)(huo)(huo)(huo)甩掉酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)內(nei)(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)；在(zai)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)臺上(shang)(shang)鋪(pu)墊幾層吸(xi)水紙，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)朝下(xia)(xia)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)力拍幾次(ci)(ci)(ci)(ci)(ci)(ci)；將(jiang)(jiang)(jiang)推(tui)薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌緩(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)至(zhi)少0.3ml注入孔(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)，浸(jin)(jin)泡(pao)1-2分(fen)(fen)(fen)鐘(zhong)(zhong)。根(gen)(gen)(gen)(gen)據(ju)需要(yao)(yao)，重復(fu)此(ci)(ci)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)數(shu)次(ci)(ci)(ci)(ci)(ci)(ci)。 自(zi)(zi)動洗(xi)(xi)板(ban)(ban)：如(ru)(ru)果(guo)有(you)(you)自(zi)(zi)動洗(xi)(xi)板(ban)(ban)機，應(ying)(ying)(ying)在(zai)熟練(lian)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)再(zai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)到(dao)正式(shi)(shi)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)(zhong)。 計(ji)(ji)(ji)算(suan) 以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)為(wei)(wei)橫(heng)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對(dui)數(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)為(wei)(wei)縱(zong)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普(pu)通(tong)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)半對(dui)數(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)繪(hui)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)，根(gen)(gen)(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)由(you)(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)查(cha)出(chu)相(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)；再(zai)乘以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)；或(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)與OD值(zhi)(zhi)計(ji)(ji)(ji)算(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)回(hui)(hui)歸(gui)方(fang)(fang)程(cheng)(cheng)式(shi)(shi)，將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)代入方(fang)(fang)程(cheng)(cheng)式(shi)(shi)，計(ji)(ji)(ji)算(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)，再(zai)乘以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)，即(ji)為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際濃(nong)(nong)(nong)度(du)(du)(du)(du)。 五、技術小(xiao)提示1.當混(hun)(hun)(hun)合(he)蛋(dan)白(bai)(bai)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)盡量(liang)(liang)(liang)(liang)輕(qing)緩(huan)，避(bi)免(mian)(mian)起泡(pao)。2. 洗(xi)(xi)滌過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)非常重要(yao)(yao)，不(bu)(bu)(bu)充分(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌易造成(cheng)假陽(yang)性(xing)。 3. 一次(ci)(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)間控制(zhi)在(zai)5分(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)(nei)，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)數(shu)量(liang)(liang)(liang)(liang)多，推(tui)薦使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)。 4. 請(qing)(qing)(qing)(qing)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)，做(zuo)復(fu)孔(kong)(kong)(kong)(kong)。 5. 如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)中(zhong)(zhong)(zhong)待(dai)測(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)質(zhi)含量(liang)(liang)(liang)(liang)過(guo)(guo)(guo)(guo)(guo)高，請(qing)(qing)(qing)(qing)先稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)再(zai)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)，計(ji)(ji)(ji)算(suan)時(shi)(shi)(shi)(shi)(shi)請(qing)(qing)(qing)(qing)乘以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)。 6. 在(zai)配(pei)(pei)(pei)(pei)制(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)，請(qing)(qing)(qing)(qing)以(yi)(yi)(yi)相(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)制(zhi)，不(bu)(bu)(bu)能混(hun)(hun)(hun)淆。 7. 底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)請(qing)(qing)(qing)(qing)避(bi)光(guang)保(bao)(bao)(bao)存(cun)(cun)(cun)。 8. 不(bu)(bu)(bu)要(yao)(yao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)其(qi)(qi)它生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)廠家(jia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)替換(huan)試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)。 六、注意事項1.收集(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)前(qian)(qian)必須清(qing)楚要(yao)(yao)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)份是否足夠穩(wen)定(ding)(ding)。對(dui)收集(ji)(ji)后(hou)(hou)當天進(jin)行(xing)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)，儲(chu)存(cun)(cun)(cun)在(zai)4 ℃備(bei)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)有(you)(you)特殊原(yuan)(yuan)因(yin)需要(yao)(yao)周期(qi)(qi)收集(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)， 將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)及(ji)(ji)時(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)裝后(hou)(hou)放(fang)在(zai)-20 ℃或(huo)(huo)(huo)(huo)(huo)-70 ℃條(tiao)(tiao)(tiao)件(jian)下(xia)(xia)保(bao)(bao)(bao)存(cun)(cun)(cun)。避(bi)免(mian)(mian)反(fan)復(fu)凍(dong)融。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)2 -8 ℃可(ke)(ke)(ke)(ke)保(bao)(bao)(bao)存(cun)(cun)(cun)48 小(xiao)時(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)(ke)(ke)保(bao)(bao)(bao)存(cun)(cun)(cun)1 個(ge)月。-70度(du)(du)(du)(du)可(ke)(ke)(ke)(ke)保(bao)(bao)(bao)存(cun)(cun)(cun)6 個(ge)月。3.部分(fen)(fen)(fen)激(ji)素(su)(su)(su)(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)需添加(jia)(jia)(jia)(jia)(jia)抑肽酶(mei)(mei)。 4.計(ji)(ji)(ji)算(suan)方(fang)(fang)法：以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)為(wei)(wei)橫(heng)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)為(wei)(wei)縱(zong)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)繪(hui)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)，根(gen)(gen)(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)由(you)(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)查(cha)出(chu)相(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)；再(zai)乘以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)；或(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)與OD值(zhi)(zhi)計(ji)(ji)(ji)算(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)回(hui)(hui)歸(gui)方(fang)(fang)程(cheng)(cheng)式(shi)(shi)，將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)代入方(fang)(fang)程(cheng)(cheng)式(shi)(shi)，計(ji)(ji)(ji)算(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)，再(zai)乘以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)，即(ji)為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際濃(nong)(nong)(nong)度(du)(du)(du)(du)。 七、試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)性(xing)能1.樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)(xian)性(xing)回(hui)(hui)歸(gui)與預(yu)期(qi)(qi)濃(nong)(nong)(nong)度(du)(du)(du)(du)相(xiang)(xiang)關(guan)系數(shu)R值(zhi)(zhi)為(wei)(wei)0.92以(yi)(yi)(yi)上(shang)(shang)。2.批內(nei)(nei)(nei)(nei)與批見應(ying)(ying)(ying)分(fen)(fen)(fen)別(bie)(bie)小(xiao)于9%和15% 八、保(bao)(bao)(bao)存(cun)(cun)(cun)條(tiao)(tiao)(tiao)件(jian)及(ji)(ji)有(you)(you)效期(qi)(qi)1.試(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)保(bao)(bao)(bao)存(cun)(cun)(cun)：2-8℃。 2．有(you)(you)效期(qi)(qi)：6個(ge)月

 小(xiao)(xiao)鼠(shu)(shu)可(ke)(ke)(ke)溶(rong)(rong)(rong)(rong)性(xing)(xing)(xing)(xing)髓(sui)(sui)系細(xi)胞(bao)觸發(fa)(fa)受(shou)(shou)體(ti)(ti)(ti)(ti)(ti)-2（sTREM-2）ELISA 檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)實(shi)(shi)(shi)驗(yan)(yan)(yan)原(yuan)理   試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)采用(yong)(yong)(yong)(yong)雙抗(kang)體(ti)(ti)(ti)(ti)(ti)一(yi)(yi)(yi)步(bu)夾心(xin)(xin)法(fa)酶(mei)(mei)(mei)(mei)(mei)(mei)聯免(mian)(mian)疫吸(xi)附試(shi)(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)（ELISA）。往預(yu)先(xian)(xian)包被(bei)(bei)脂(zhi)(zhi)肪酸(suan)結(jie)(jie)合蛋(dan)白(bai)1(FABP1)捕(bu)獲抗(kang)體(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被(bei)(bei)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，依次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)體(ti)(ti)(ti)(ti)(ti)，經過(guo)(guo)(guo)(guo)(guo)溫育(yu)并徹底(di)(di)(di)洗(xi)(xi)滌。用(yong)(yong)(yong)(yong)底(di)(di)(di)物(wu)(wu)(wu)(wu)TMB顯(xian)色，TMB在(zai)(zai)(zai)(zai)(zai)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)下(xia)轉(zhuan)化(hua)(hua)成(cheng)(cheng)藍色，并在(zai)(zai)(zai)(zai)(zai)酸(suan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)下(xia)轉(zhuan)化(hua)(hua)成(cheng)(cheng)＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)色。顏色的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂(zhi)(zhi)肪酸(suan)結(jie)(jie)合蛋(dan)白(bai)1(FABP1)呈(cheng)正(zheng)(zheng)相(xiang)(xiang)關。用(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)(zai)(zai)(zai)450nm 波長下(xia)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)吸(xi)光度(du)(du)(du)(du)（OD 值(zhi)），計(ji)算(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)。保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)條(tiao)件及(ji)(ji)有(you)(you)(you)(you)效(xiao)期(qi)(qi)本(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)體(ti)(ti)(ti)(ti)(ti)外定(ding)(ding)(ding)(ding)量(liang)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)血(xue)(xue)(xue)清(qing)(qing)(qing)、血(xue)(xue)(xue)漿(jiang)、組織(zhi)勻(yun)(yun)漿(jiang)及(ji)(ji)相(xiang)(xiang)關液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)脂(zhi)(zhi)肪酸(suan)結(jie)(jie)合蛋(dan)白(bai)1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)。有(you)(you)(you)(you)效(xiao)期(qi)(qi)：6個(ge)月(yue)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)條(tiao)件：2-8℃小(xiao)(xiao)鼠(shu)(shu)可(ke)(ke)(ke)溶(rong)(rong)(rong)(rong)性(xing)(xing)(xing)(xing)髓(sui)(sui)系細(xi)胞(bao)觸發(fa)(fa)受(shou)(shou)體(ti)(ti)(ti)(ti)(ti)-2（sTREM-2）ELISA 檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)一(yi)(yi)(yi)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采集(ji)及(ji)(ji)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun) 1.血(xue)(xue)(xue)清(qing)(qing)(qing)：全血(xue)(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)請于(yu)(yu)(yu)室(shi)溫放置(zhi)(zhi)2小(xiao)(xiao)時(shi)(shi)或(huo)(huo)(huo)4℃過(guo)(guo)(guo)(guo)(guo)一(yi)(yi)(yi)晚后(hou)(hou)于(yu)(yu)(yu)1000 x g離心(xin)(xin)20分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，取(qu)上(shang)清(qing)(qing)(qing)即可(ke)(ke)(ke)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)放于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)避(bi)免(mian)(mian)反(fan)(fan)復(fu)(fu)(fu)凍(dong)(dong)融。 2.血(xue)(xue)(xue)漿(jiang)：可(ke)(ke)(ke)用(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)肝素(su)(su)作(zuo)(zuo)(zuo)(zuo)為(wei)(wei)(wei)抗(kang)凝(ning)劑(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)采集(ji)后(hou)(hou)30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內于(yu)(yu)(yu)2 - 8° C 1000 x g離心(xin)(xin)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，或(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)放于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)避(bi)免(mian)(mian)反(fan)(fan)復(fu)(fu)(fu)凍(dong)(dong)融。 3.細(xi)胞(bao)培養(yang)物(wu)(wu)(wu)(wu)上(shang)清(qing)(qing)(qing)或(huo)(huo)(huo)其它生(sheng)(sheng)物(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)：1000 x g離心(xin)(xin)20分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，取(qu)上(shang)清(qing)(qing)(qing)即可(ke)(ke)(ke)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)放于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)避(bi)免(mian)(mian)反(fan)(fan)復(fu)(fu)(fu)凍(dong)(dong)融。 注(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)溶(rong)(rong)(rong)(rong)血(xue)(xue)(xue)會影響檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)果(guo)，因(yin)此(ci)(ci)溶(rong)(rong)(rong)(rong)血(xue)(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)不(bu)宜進行(xing)此(ci)(ci)項檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)。二、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)   首先(xian)(xian)通過(guo)(guo)(guo)(guo)(guo)文獻檢(jian)(jian)(jian)(jian)索(suo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方式(shi)了解(jie)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含量(liang)，確定(ding)(ding)(ding)(ding)適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)。只有(you)(you)(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍內，檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)(jie)果(guo)才(cai)是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)(guo)程中(zhong)(zhong)(zhong)，應(ying)(ying)(ying)做好(hao)(hao)詳細(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)錄。計(ji)算(suan)(suan)濃(nong)(nong)(nong)度(du)(du)(du)(du)時(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)應(ying)(ying)(ying)再乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：2瓶，每(mei)(mei)(mei)瓶臨用(yong)(yong)(yong)(yong)前(qian)以(yi)(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)(zhi)1ml，蓋好(hao)(hao)后(hou)(hou)靜置(zhi)(zhi)10分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)上(shang)，然后(hou)(hou)反(fan)(fan)復(fu)(fu)(fu)顛倒/搓動(dong)(dong)以(yi)(yi)(yi)(yi)(yi)助溶(rong)(rong)(rong)(rong)解(jie)，其濃(nong)(nong)(nong)度(du)(du)(du)(du)為(wei)(wei)(wei)300 pg/ml，做系列倍(bei)(bei)比稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)，分(fen)(fen)(fen)(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)直(zhi)(zhi)接作(zuo)(zuo)(zuo)(zuo)為(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)度(du)(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)前(qian)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。 如(ru)(ru)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取(qu)0.5ml（不(bu)要(yao)(yao)(yao)少(shao)(shao)于(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)含0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中(zhong)(zhong)(zhong)，混(hun)勻(yun)(yun)即可(ke)(ke)(ke)，其余濃(nong)(nong)(nong)度(du)(du)(du)(du)以(yi)(yi)(yi)(yi)(yi)此(ci)(ci)類推。2.生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)抗(kang)體(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)： 臨用(yong)(yong)(yong)(yong)前(qian)以(yi)(yi)(yi)(yi)(yi)生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)抗(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)根(gen)(gen)據預(yu)先(xian)(xian)計(ji)算(suan)(suan)好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)所(suo)需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)際(ji)(ji)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)抗(kang)體(ti)(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)抗(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)勻(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前(qian)一(yi)(yi)(yi)小(xiao)(xiao)時(shi)(shi)內配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。3.辣(la)(la)根(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)親(qin)和素(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)： 臨用(yong)(yong)(yong)(yong)前(qian)以(yi)(yi)(yi)(yi)(yi)辣(la)(la)根(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)親(qin)和素(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)根(gen)(gen)據預(yu)先(xian)(xian)計(ji)算(suan)(suan)好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)所(suo)需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)際(ji)(ji)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl辣(la)(la)根(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)親(qin)和素(su)(su)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl辣(la)(la)根(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)親(qin)和素(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)勻(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前(qian)一(yi)(yi)(yi)小(xiao)(xiao)時(shi)(shi)內配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。 操作(zuo)(zuo)(zuo)(zuo)步(bu)驟 實(shi)(shi)(shi)驗(yan)(yan)(yan)開始前(qian)，請提前(qian)配(pei)(pei)置(zhi)(zhi)好(hao)(hao)所(suo)有(you)(you)(you)(you)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)或(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)，均需混(hun)勻(yun)(yun)，混(hun)勻(yun)(yun)時(shi)(shi)盡(jin)量(liang)避(bi)免(mian)(mian)起(qi)(qi)泡(pao)(pao)。每(mei)(mei)(mei)次(ci)(ci)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)該做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)。如(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)過(guo)(guo)(guo)(guo)(guo)高時(shi)(shi)，用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)進行(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)(yi)使(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)范圍。 小(xiao)(xiao)鼠(shu)(shu)可(ke)(ke)(ke)溶(rong)(rong)(rong)(rong)性(xing)(xing)(xing)(xing)髓(sui)(sui)系細(xi)胞(bao)觸發(fa)(fa)受(shou)(shou)體(ti)(ti)(ti)(ti)(ti)-2（sTREM-2）ELISA 檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)三、操作(zuo)(zuo)(zuo)(zuo)步(bu)驟1.加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)別(bie)設空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)、待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)意(yi)不(bu)要(yao)(yao)(yao)有(you)(you)(you)(you)氣泡(pao)(pao)，加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)(yu)(yu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)底(di)(di)(di)部，盡(jin)量(liang)不(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁(bi)，輕(qing)輕(qing)晃動(dong)(dong)混(hun)勻(yun)(yun)，酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)蓋或(huo)(huo)(huo)覆膜，37℃反(fan)(fan)應(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。 為(wei)(wei)(wei)保(bao)(bao)(bao)(bao)證(zheng)實(shi)(shi)(shi)驗(yan)(yan)(yan)結(jie)(jie)果(guo)有(you)(you)(you)(you)效(xiao)性(xing)(xing)(xing)(xing)，每(mei)(mei)(mei)次(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)請使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)新(xin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。2.棄(qi)(qi)去(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩(shuai)干(gan)(gan)(gan)(gan)，不(bu)用(yong)(yong)(yong)(yong)洗(xi)(xi)滌。每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)抗(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取(qu)1μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)抗(kang)體(ti)(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)抗(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)勻(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前(qian)一(yi)(yi)(yi)小(xiao)(xiao)時(shi)(shi)內配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。 3.溫育(yu)60分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)，棄(qi)(qi)去(qu)(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩(shuai)干(gan)(gan)(gan)(gan)，洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)3次(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)浸泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)干(gan)(gan)(gan)(gan)。 4.每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)辣(la)(la)根(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)親(qin)和素(su)(su)工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同(tong)生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)抗(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。 5.溫育(yu)60分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)，棄(qi)(qi)去(qu)(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩(shuai)干(gan)(gan)(gan)(gan)，洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)5次(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)浸泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)干(gan)(gan)(gan)(gan)。 6.依序(xu)(xu)每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)(di)(di)物(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)光顯(xian)色（30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內，此(ci)(ci)時(shi)(shi)肉眼可(ke)(ke)(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)有(you)(you)(you)(you)明顯(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯(ti)度(du)(du)(du)(du)藍色，后(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)梯(ti)度(du)(du)(du)(du)不(bu)明顯(xian)，即可(ke)(ke)(ke)終(zhong)止(zhi)(zhi)）。 7.依序(xu)(xu)每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)(zhi)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止(zhi)(zhi)反(fan)(fan)應(ying)(ying)(ying)（此(ci)(ci)時(shi)(shi)藍色立轉(zhuan)黃(huang)色）。終(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)順(shun)(shun)序(xu)(xu)應(ying)(ying)(ying)盡(jin)量(liang)與(yu)底(di)(di)(di)物(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)順(shun)(shun)序(xu)(xu)相(xiang)(xiang)同(tong)。為(wei)(wei)(wei)了保(bao)(bao)(bao)(bao)證(zheng)實(shi)(shi)(shi)驗(yan)(yan)(yan)結(jie)(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)(xing)(xing)，底(di)(di)(di)物(wu)(wu)(wu)(wu)反(fan)(fan)應(ying)(ying)(ying)時(shi)(shi)間到后(hou)(hou)應(ying)(ying)(ying)盡(jin)快(kuai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)終(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 8.用(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)聯儀在(zai)(zai)(zai)(zai)(zai)450nm波長依序(xu)(xu)測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光密度(du)(du)(du)(du)（OD值(zhi)）。 在(zai)(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)內進行(xing)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)。 四、試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)小(xiao)(xiao)技巧1. 用(yong)(yong)(yong)(yong)戶在(zai)(zai)(zai)(zai)(zai)初次(ci)(ci)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)時(shi)(shi)，應(ying)(ying)(ying)將(jiang)(jiang)(jiang)各種試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)管離心(xin)(xin)數(shu)分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，以(yi)(yi)(yi)(yi)(yi)便(bian)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)集(ji)中(zhong)(zhong)(zhong)到管底(di)(di)(di)。2. 每(mei)(mei)(mei)次(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)留一(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)(zuo)(zuo)為(wei)(wei)(wei)空白(bai)調零孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，該孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)任何試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)，只是**加(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)(di)(di)物(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)及(ji)(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)時(shi)(shi)先(xian)(xian)用(yong)(yong)(yong)(yong)此(ci)(ci)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)調OD值(zhi)至(zhi)(zhi)零。 3. 為(wei)(wei)(wei)防止(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發(fa)(fa)，試(shi)(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)時(shi)(shi)將(jiang)(jiang)(jiang)反(fan)(fan)應(ying)(ying)(ying)板(ban)(ban)(ban)(ban)(ban)(ban)放于(yu)(yu)(yu)鋪有(you)(you)(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)(he)(he)(he)內，酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)蓋或(huo)(huo)(huo)覆膜。 4. 未使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)(ban)或(huo)(huo)(huo)者(zhe)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)，請于(yu)(yu)(yu)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)抗(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)(la)根(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)親(qin)和素(su)(su)工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)請依據所(suo)需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)配(pei)(pei)置(zhi)(zhi)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)。請勿重(zhong)復(fu)(fu)(fu)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)抗(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)、辣(la)(la)根(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)親(qin)和素(su)(su)工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5. 建議檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)均設雙孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)，以(yi)(yi)(yi)(yi)(yi)保(bao)(bao)(bao)(bao)證(zheng)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)(xing)(xing)。 洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)方法(fa) 手工(gong)(gong)洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)方法(fa)：吸(xi)去(qu)(qu)（不(bu)可(ke)(ke)(ke)觸及(ji)(ji)板(ban)(ban)(ban)(ban)(ban)(ban)壁(bi)）或(huo)(huo)(huo)甩(shuai)掉酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)(ban)內的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)；在(zai)(zai)(zai)(zai)(zai)實(shi)(shi)(shi)驗(yan)(yan)(yan)臺上(shang)鋪墊幾層吸(xi)水(shui)紙，酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)(ban)(ban)朝下(xia)用(yong)(yong)(yong)(yong)力拍幾次(ci)(ci)；將(jiang)(jiang)(jiang)推薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌緩沖液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)至(zhi)(zhi)少(shao)(shao)0.3ml注(zhu)入(ru)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內，浸泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。根(gen)(gen)據需要(yao)(yao)(yao)，重(zhong)復(fu)(fu)(fu)此(ci)(ci)過(guo)(guo)(guo)(guo)(guo)程數(shu)次(ci)(ci)。 自(zi)(zi)動(dong)(dong)洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)：如(ru)(ru)果(guo)有(you)(you)(you)(you)自(zi)(zi)動(dong)(dong)洗(xi)(xi)板(ban)(ban)(ban)(ban)(ban)(ban)機，應(ying)(ying)(ying)在(zai)(zai)(zai)(zai)(zai)熟練使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)后(hou)(hou)再用(yong)(yong)(yong)(yong)到正(zheng)(zheng)式(shi)實(shi)(shi)(shi)驗(yan)(yan)(yan)過(guo)(guo)(guo)(guo)(guo)程中(zhong)(zhong)(zhong)。 計(ji)算(suan)(suan) 以(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)為(wei)(wei)(wei)橫坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)為(wei)(wei)(wei)縱(zong)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普(pu)通坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)(zai)(zai)半對數(shu)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)，根(gen)(gen)據樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)查(cha)出(chu)相(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)；再乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)與(yu)OD值(zhi)計(ji)算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)(zhi)線(xian)(xian)回歸方程式(shi)，將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代入(ru)方程式(shi)，計(ji)算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)，再乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)，即為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際(ji)(ji)濃(nong)(nong)(nong)度(du)(du)(du)(du)。 五、技術小(xiao)(xiao)提示(shi)1.當混(hun)合蛋(dan)白(bai)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)應(ying)(ying)(ying)盡(jin)量(liang)輕(qing)緩，避(bi)免(mian)(mian)起(qi)(qi)泡(pao)(pao)。2. 洗(xi)(xi)滌過(guo)(guo)(guo)(guo)(guo)程非常重(zhong)要(yao)(yao)(yao)，不(bu)充(chong)分(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌易造成(cheng)(cheng)假陽性(xing)(xing)(xing)(xing)。 3. 一(yi)(yi)(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)間控制(zhi)(zhi)(zhi)(zhi)(zhi)在(zai)(zai)(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)數(shu)量(liang)多，推薦使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)。 4. 請每(mei)(mei)(mei)次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)，做復(fu)(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。 5. 如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)質含量(liang)過(guo)(guo)(guo)(guo)(guo)高，請先(xian)(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)再測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)，計(ji)算(suan)(suan)時(shi)(shi)請乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)。 6. 在(zai)(zai)(zai)(zai)(zai)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)，請以(yi)(yi)(yi)(yi)(yi)相(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，不(bu)能(neng)混(hun)淆。 7. 底(di)(di)(di)物(wu)(wu)(wu)(wu)請避(bi)光保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。 8. 不(bu)要(yao)(yao)(yao)用(yong)(yong)(yong)(yong)其它生(sheng)(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)替換試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)。 六、注(zhu)意(yi)事項1.收集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)前(qian)必須(xu)清(qing)(qing)(qing)楚要(yao)(yao)(yao)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)份(fen)是否(fou)足夠(gou)穩定(ding)(ding)(ding)(ding)。對收集(ji)后(hou)(hou)當天(tian)進行(xing)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)，儲(chu)存(cun)(cun)(cun)(cun)在(zai)(zai)(zai)(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)，如(ru)(ru)有(you)(you)(you)(you)特殊原(yuan)因(yin)需要(yao)(yao)(yao)周期(qi)(qi)收集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)， 將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)及(ji)(ji)時(shi)(shi)分(fen)(fen)(fen)(fen)(fen)裝后(hou)(hou)放在(zai)(zai)(zai)(zai)(zai)-20 ℃或(huo)(huo)(huo)-70 ℃條(tiao)件下(xia)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。避(bi)免(mian)(mian)反(fan)(fan)復(fu)(fu)(fu)凍(dong)(dong)融。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)48 小(xiao)(xiao)時(shi)(shi)，-20 ℃可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)1 個(ge)月(yue)。-70度(du)(du)(du)(du)可(ke)(ke)(ke)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)6 個(ge)月(yue)。3.部分(fen)(fen)(fen)(fen)(fen)激素(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)需添加(jia)(jia)(jia)(jia)(jia)(jia)(jia)抑肽酶(mei)(mei)(mei)(mei)(mei)(mei)。 4.計(ji)算(suan)(suan)方法(fa)：以(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)為(wei)(wei)(wei)橫坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)為(wei)(wei)(wei)縱(zong)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)(zai)(zai)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)，根(gen)(gen)據樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)查(cha)出(chu)相(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)；再乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)與(yu)OD值(zhi)計(ji)算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)(zhi)線(xian)(xian)回歸方程式(shi)，將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代入(ru)方程式(shi)，計(ji)算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)，再乘(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)，即為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際(ji)(ji)濃(nong)(nong)(nong)度(du)(du)(du)(du)。 七、試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)性(xing)(xing)(xing)(xing)能(neng)1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)性(xing)(xing)(xing)(xing)回歸與(yu)預(yu)期(qi)(qi)濃(nong)(nong)(nong)度(du)(du)(du)(du)相(xiang)(xiang)關系數(shu)R值(zhi)為(wei)(wei)(wei)0.92以(yi)(yi)(yi)(yi)(yi)上(shang)。2.批內與(yu)批見應(ying)(ying)(ying)分(fen)(fen)(fen)(fen)(fen)別(bie)小(xiao)(xiao)于(yu)(yu)(yu)9%和15% 八、保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)條(tiao)件及(ji)(ji)有(you)(you)(you)(you)效(xiao)期(qi)(qi)1.試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)：2-8℃。 2．有(you)(you)(you)(you)效(xiao)期(qi)(qi)：6個(ge)月(yue)

 小(xiao)(xiao)(xiao)鼠(shu)磷酸(suan)化 Tau 蛋(dan)白(bai)（p-Tau）ELISA 檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)實(shi)(shi)(shi)驗(yan)(yan)(yan)原(yuan)理   試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)采用(yong)(yong)(yong)(yong)(yong)雙抗(kang)(kang)體(ti)(ti)(ti)(ti)一(yi)(yi)步夾心法酶(mei)(mei)聯免(mian)(mian)疫吸附試(shi)(shi)驗(yan)(yan)(yan)（ELISA）。往預(yu)先(xian)(xian)包被脂肪酸(suan)結(jie)(jie)合(he)蛋(dan)白(bai)1(FABP1)捕獲抗(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中，依(yi)次(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)入標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)抗(kang)(kang)體(ti)(ti)(ti)(ti)，經過(guo)(guo)溫育并(bing)徹底(di)(di)洗(xi)(xi)(xi)滌(di)。用(yong)(yong)(yong)(yong)(yong)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯色(se)(se)(se)，TMB在(zai)(zai)過(guo)(guo)氧(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化下(xia)轉(zhuan)化成(cheng)(cheng)藍(lan)色(se)(se)(se)，并(bing)在(zai)(zai)酸(suan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)下(xia)轉(zhuan)化成(cheng)(cheng)＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)色(se)(se)(se)。顏色(se)(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺(qian)和(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)中的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂肪酸(suan)結(jie)(jie)合(he)蛋(dan)白(bai)1(FABP1)呈正相關(guan)。用(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)450nm 波(bo)長(chang)下(xia)測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)吸光(guang)(guang)度(du)(du)(du)(du)(du)(du)(du)（OD 值(zhi)(zhi)(zhi)），計(ji)(ji)(ji)算(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。保(bao)(bao)存(cun)(cun)(cun)(cun)條件(jian)及(ji)有(you)(you)(you)(you)效(xiao)(xiao)期(qi)本(ben)(ben)(ben)(ben)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)體(ti)(ti)(ti)(ti)外(wai)定(ding)(ding)(ding)量(liang)(liang)(liang)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)血(xue)清(qing)、血(xue)漿、組織勻漿及(ji)相關(guan)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)中脂肪酸(suan)結(jie)(jie)合(he)蛋(dan)白(bai)1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)(liang)。有(you)(you)(you)(you)效(xiao)(xiao)期(qi)：6個(ge)(ge)月保(bao)(bao)存(cun)(cun)(cun)(cun)條件(jian)：2-8℃小(xiao)(xiao)(xiao)鼠(shu)磷酸(suan)化 Tau 蛋(dan)白(bai)（p-Tau）ELISA 檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)一(yi)(yi)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采集及(ji)保(bao)(bao)存(cun)(cun)(cun)(cun) 1.血(xue)清(qing)：全血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)請(qing)于(yu)(yu)(yu)(yu)室溫放(fang)置2小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)4℃過(guo)(guo)一(yi)(yi)晚(wan)后于(yu)(yu)(yu)(yu)1000 x g離心20分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)，取上(shang)(shang)清(qing)即(ji)(ji)(ji)(ji)可(ke)(ke)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)(ying)避(bi)(bi)(bi)免(mian)(mian)反(fan)復(fu)(fu)凍(dong)融。 2.血(xue)漿：可(ke)(ke)用(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)肝(gan)素(su)(su)(su)(su)(su)(su)作(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)(wei)抗(kang)(kang)凝劑(ji)(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)采集后30分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)內(nei)于(yu)(yu)(yu)(yu)2 - 8° C 1000 x g離心15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)，或(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)(ying)避(bi)(bi)(bi)免(mian)(mian)反(fan)復(fu)(fu)凍(dong)融。 3.細(xi)胞培養(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)清(qing)或(huo)(huo)(huo)其(qi)它(ta)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)：1000 x g離心20分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)，取上(shang)(shang)清(qing)即(ji)(ji)(ji)(ji)可(ke)(ke)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)(cun)(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)(ying)避(bi)(bi)(bi)免(mian)(mian)反(fan)復(fu)(fu)凍(dong)融。 注(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)溶(rong)(rong)(rong)血(xue)會影響檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)結(jie)(jie)果(guo)(guo)(guo)(guo)，因此(ci)(ci)溶(rong)(rong)(rong)血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)不宜進(jin)行(xing)此(ci)(ci)項檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)。二(er)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)   首(shou)先(xian)(xian)通過(guo)(guo)文獻檢(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)式了解待(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含量(liang)(liang)(liang)，確(que)定(ding)(ding)(ding)適當(dang)(dang)(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)。只有(you)(you)(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范(fan)圍內(nei)，檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)(jie)果(guo)(guo)(guo)(guo)才是(shi)準(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)程(cheng)(cheng)中，應(ying)(ying)(ying)(ying)(ying)做(zuo)好(hao)(hao)詳細(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)錄。計(ji)(ji)(ji)算(suan)(suan)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)應(ying)(ying)(ying)(ying)(ying)再(zai)(zai)乘以(yi)(yi)(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：2瓶(ping)，每(mei)(mei)(mei)(mei)瓶(ping)臨用(yong)(yong)(yong)(yong)(yong)前(qian)以(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至1ml，蓋好(hao)(hao)后靜置10分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)以(yi)(yi)(yi)上(shang)(shang)，然后反(fan)復(fu)(fu)顛倒/搓動以(yi)(yi)(yi)助溶(rong)(rong)(rong)解，其(qi)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)(wei)300 pg/ml，做(zuo)系列(lie)倍(bei)(bei)比(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后，分(fen)(fen)(fen)(fen)(fen)(fen)別稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)直接作(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)(yong)前(qian)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)內(nei)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 如(ru)(ru)(ru)(ru)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)：取0.5ml（不要(yao)(yao)(yao)少于(yu)(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)入含0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中，混(hun)(hun)勻即(ji)(ji)(ji)(ji)可(ke)(ke)，其(qi)余濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)以(yi)(yi)(yi)此(ci)(ci)類(lei)推。2.生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)： 臨用(yong)(yong)(yong)(yong)(yong)前(qian)以(yi)(yi)(yi)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)根(gen)(gen)(gen)(gen)據預(yu)先(xian)(xian)計(ji)(ji)(ji)算(suan)(suan)好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)所(suo)(suo)需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)際(ji)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)多(duo)(duo)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)(ru)10μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)勻，在(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)一(yi)(yi)小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內(nei)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。3.辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)： 臨用(yong)(yong)(yong)(yong)(yong)前(qian)以(yi)(yi)(yi)辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)根(gen)(gen)(gen)(gen)據預(yu)先(xian)(xian)計(ji)(ji)(ji)算(suan)(suan)好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)所(suo)(suo)需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)際(ji)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)多(duo)(duo)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)(ru)10μl辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)(su)(su)加(jia)(jia)(jia)(jia)(jia)990μl辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)勻，在(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)一(yi)(yi)小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內(nei)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)。 操作(zuo)(zuo)(zuo)步驟 實(shi)(shi)(shi)驗(yan)(yan)(yan)開始(shi)前(qian)，請(qing)提(ti)前(qian)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)置好(hao)(hao)所(suo)(suo)有(you)(you)(you)(you)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)或(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，均需(xu)(xu)混(hun)(hun)勻，混(hun)(hun)勻時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盡量(liang)(liang)(liang)避(bi)(bi)(bi)免(mian)(mian)起(qi)泡(pao)。每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)(ying)該(gai)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)。如(ru)(ru)(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)過(guo)(guo)高時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)進(jin)行(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)使(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)符合(he)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)范(fan)圍。 小(xiao)(xiao)(xiao)鼠(shu)磷酸(suan)化 Tau 蛋(dan)白(bai)（p-Tau）ELISA 檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)三、操作(zuo)(zuo)(zuo)步驟1.加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)別設(she)空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、待(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)別加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)待(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)意(yi)不要(yao)(yao)(yao)有(you)(you)(you)(you)氣(qi)泡(pao)，加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)于(yu)(yu)(yu)(yu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底(di)(di)部，盡量(liang)(liang)(liang)不觸(chu)及(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)(qing)(qing)輕(qing)(qing)(qing)晃動混(hun)(hun)勻，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)上(shang)(shang)蓋或(huo)(huo)(huo)覆膜(mo)，37℃反(fan)應(ying)(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)。 為(wei)(wei)(wei)(wei)(wei)保(bao)(bao)證實(shi)(shi)(shi)驗(yan)(yan)(yan)結(jie)(jie)果(guo)(guo)(guo)(guo)有(you)(you)(you)(you)效(xiao)(xiao)性(xing)(xing)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)請(qing)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。2.棄(qi)去液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干(gan)，不用(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)(xi)滌(di)。每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)(hun)勻，在(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)一(yi)(yi)小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內(nei)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)。 3.溫育60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)后，棄(qi)去孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干(gan)，洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)3次(ci)(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)干(gan)。 4.每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同(tong)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)。 5.溫育60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)后，棄(qi)去孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干(gan)，洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)5次(ci)(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)干(gan)。 6.依(yi)序(xu)每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)(bi)(bi)光(guang)(guang)顯色(se)(se)(se)（30分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)內(nei)，此(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)肉眼可(ke)(ke)見(jian)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)有(you)(you)(you)(you)明顯的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯(ti)度(du)(du)(du)(du)(du)(du)(du)藍(lan)色(se)(se)(se)，后3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)梯(ti)度(du)(du)(du)(du)(du)(du)(du)不明顯，即(ji)(ji)(ji)(ji)可(ke)(ke)終(zhong)止）。 7.依(yi)序(xu)每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)終(zhong)止溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止反(fan)應(ying)(ying)(ying)(ying)(ying)（此(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)藍(lan)色(se)(se)(se)立轉(zhuan)黃(huang)色(se)(se)(se)）。終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)入順(shun)序(xu)應(ying)(ying)(ying)(ying)(ying)盡量(liang)(liang)(liang)與(yu)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)入順(shun)序(xu)相同(tong)。為(wei)(wei)(wei)(wei)(wei)了保(bao)(bao)證實(shi)(shi)(shi)驗(yan)(yan)(yan)結(jie)(jie)果(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)確(que)性(xing)(xing)，底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)應(ying)(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)間(jian)(jian)到后應(ying)(ying)(ying)(ying)(ying)盡快(kuai)加(jia)(jia)(jia)(jia)(jia)入終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 8.用(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)聯儀在(zai)(zai)450nm波(bo)長(chang)依(yi)序(xu)測(ce)(ce)(ce)(ce)量(liang)(liang)(liang)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)(guang)密(mi)度(du)(du)(du)(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)）。 在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)以(yi)(yi)(yi)內(nei)進(jin)行(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)。 四、試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)小(xiao)(xiao)(xiao)技巧1. 用(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)初(chu)次(ci)(ci)(ci)(ci)(ci)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)(ying)將(jiang)各種試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)管離心數(shu)(shu)(shu)分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)，以(yi)(yi)(yi)便試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)集中到管底(di)(di)。2. 每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)留(liu)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)(wei)空白(bai)調零孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，該(gai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不加(jia)(jia)(jia)(jia)(jia)任何試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，只是(shi)**加(jia)(jia)(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)及(ji)2N H2SO4。測(ce)(ce)(ce)(ce)量(liang)(liang)(liang)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)先(xian)(xian)用(yong)(yong)(yong)(yong)(yong)此(ci)(ci)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)調OD值(zhi)(zhi)(zhi)至零。 3. 為(wei)(wei)(wei)(wei)(wei)防止樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)驗(yan)(yan)(yan)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)反(fan)應(ying)(ying)(ying)(ying)(ying)板(ban)(ban)(ban)(ban)放(fang)于(yu)(yu)(yu)(yu)鋪有(you)(you)(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密(mi)閉盒(he)(he)內(nei)，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)上(shang)(shang)蓋或(huo)(huo)(huo)覆膜(mo)。 4. 未使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)或(huo)(huo)(huo)者試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，請(qing)于(yu)(yu)(yu)(yu)2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)請(qing)依(yi)據所(suo)(suo)需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)置使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。請(qing)勿(wu)重(zhong)復(fu)(fu)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)、辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)(su)(su)(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5. 建議檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)均設(she)雙孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，以(yi)(yi)(yi)保(bao)(bao)證檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)結(jie)(jie)果(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)確(que)性(xing)(xing)。 洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)方(fang)法 手工(gong)(gong)(gong)洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)方(fang)法：吸去（不可(ke)(ke)觸(chu)及(ji)板(ban)(ban)(ban)(ban)壁）或(huo)(huo)(huo)甩(shuai)(shuai)掉(diao)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)內(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)；在(zai)(zai)實(shi)(shi)(shi)驗(yan)(yan)(yan)臺(tai)上(shang)(shang)鋪墊幾層吸水(shui)紙，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)朝下(xia)用(yong)(yong)(yong)(yong)(yong)力拍幾次(ci)(ci)(ci)(ci)(ci)；將(jiang)推薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)(xi)滌(di)緩沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)至少0.3ml注(zhu)入孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)，浸泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)。根(gen)(gen)(gen)(gen)據需(xu)(xu)要(yao)(yao)(yao)，重(zhong)復(fu)(fu)此(ci)(ci)過(guo)(guo)程(cheng)(cheng)數(shu)(shu)(shu)次(ci)(ci)(ci)(ci)(ci)。 自動洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)：如(ru)(ru)(ru)(ru)果(guo)(guo)(guo)(guo)有(you)(you)(you)(you)自動洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)機，應(ying)(ying)(ying)(ying)(ying)在(zai)(zai)熟練使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)后再(zai)(zai)用(yong)(yong)(yong)(yong)(yong)到正式實(shi)(shi)(shi)驗(yan)(yan)(yan)過(guo)(guo)程(cheng)(cheng)中。 計(ji)(ji)(ji)算(suan)(suan) 以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)(wei)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對(dui)數(shu)(shu)(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)(wei)縱(zong)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)半對(dui)數(shu)(shu)(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)繪出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)，根(gen)(gen)(gen)(gen)據樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)查出(chu)(chu)(chu)相應(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)；再(zai)(zai)乘以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)(ji)(ji)算(suan)(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)回歸方(fang)程(cheng)(cheng)式，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入方(fang)程(cheng)(cheng)式，計(ji)(ji)(ji)算(suan)(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)，再(zai)(zai)乘以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)，即(ji)(ji)(ji)(ji)為(wei)(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際(ji)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。 五、技術小(xiao)(xiao)(xiao)提(ti)示1.當(dang)(dang)(dang)混(hun)(hun)合(he)蛋(dan)白(bai)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)盡量(liang)(liang)(liang)輕(qing)(qing)(qing)緩，避(bi)(bi)(bi)免(mian)(mian)起(qi)泡(pao)。2. 洗(xi)(xi)(xi)滌(di)過(guo)(guo)程(cheng)(cheng)非常(chang)重(zhong)要(yao)(yao)(yao)，不充分(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)(xi)滌(di)易造成(cheng)(cheng)假(jia)陽性(xing)(xing)。 3. 一(yi)(yi)次(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)間(jian)(jian)控制(zhi)(zhi)(zhi)在(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)內(nei)，如(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)(shu)(shu)量(liang)(liang)(liang)多(duo)(duo)，推薦使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)排槍(qiang)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。 4. 請(qing)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)，做(zuo)復(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。 5. 如(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中待(dai)測(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)(liang)(liang)過(guo)(guo)高，請(qing)先(xian)(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后再(zai)(zai)測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，計(ji)(ji)(ji)算(suan)(suan)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)請(qing)乘以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)。 6. 在(zai)(zai)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)以(yi)(yi)(yi)相應(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)，不能混(hun)(hun)淆(xiao)。 7. 底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)避(bi)(bi)(bi)光(guang)(guang)保(bao)(bao)存(cun)(cun)(cun)(cun)。 8. 不要(yao)(yao)(yao)用(yong)(yong)(yong)(yong)(yong)其(qi)它(ta)生(sheng)(sheng)(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)替(ti)換試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)中的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)。 六(liu)、注(zhu)意(yi)事項1.收(shou)集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)前(qian)必須清(qing)楚要(yao)(yao)(yao)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)份是(shi)否足夠穩(wen)定(ding)(ding)(ding)。對(dui)收(shou)集后當(dang)(dang)(dang)天進(jin)行(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)，儲存(cun)(cun)(cun)(cun)在(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)(ru)(ru)有(you)(you)(you)(you)特殊(shu)原(yuan)因需(xu)(xu)要(yao)(yao)(yao)周(zhou)期(qi)收(shou)集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)， 將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)及(ji)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)(fen)裝后放(fang)在(zai)(zai)-20 ℃或(huo)(huo)(huo)-70 ℃條件(jian)下(xia)保(bao)(bao)存(cun)(cun)(cun)(cun)。避(bi)(bi)(bi)免(mian)(mian)反(fan)復(fu)(fu)凍(dong)融。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)保(bao)(bao)存(cun)(cun)(cun)(cun)48 小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)保(bao)(bao)存(cun)(cun)(cun)(cun)1 個(ge)(ge)月。-70度(du)(du)(du)(du)(du)(du)(du)可(ke)(ke)保(bao)(bao)存(cun)(cun)(cun)(cun)6 個(ge)(ge)月。3.部分(fen)(fen)(fen)(fen)(fen)(fen)激素(su)(su)(su)(su)(su)(su)類(lei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)需(xu)(xu)添加(jia)(jia)(jia)(jia)(jia)抑肽(tai)酶(mei)(mei)。 4.計(ji)(ji)(ji)算(suan)(suan)方(fang)法：以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)(wei)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)(wei)縱(zong)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)繪出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)，根(gen)(gen)(gen)(gen)據樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)查出(chu)(chu)(chu)相應(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)；再(zai)(zai)乘以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)(ji)(ji)算(suan)(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)回歸方(fang)程(cheng)(cheng)式，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入方(fang)程(cheng)(cheng)式，計(ji)(ji)(ji)算(suan)(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)，再(zai)(zai)乘以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)，即(ji)(ji)(ji)(ji)為(wei)(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際(ji)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。 七、試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)性(xing)(xing)能1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)性(xing)(xing)回歸與(yu)預(yu)期(qi)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)相關(guan)系數(shu)(shu)(shu)R值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)(wei)0.92以(yi)(yi)(yi)上(shang)(shang)。2.批(pi)內(nei)與(yu)批(pi)見(jian)應(ying)(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)(fen)(fen)別小(xiao)(xiao)(xiao)于(yu)(yu)(yu)(yu)9%和(he)(he)(he)15% 八、保(bao)(bao)存(cun)(cun)(cun)(cun)條件(jian)及(ji)有(you)(you)(you)(you)效(xiao)(xiao)期(qi)1.試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)保(bao)(bao)存(cun)(cun)(cun)(cun)：2-8℃。 2．有(you)(you)(you)(you)效(xiao)(xiao)期(qi)：6個(ge)(ge)月

 小(xiao)(xiao)(xiao)鼠神經絲蛋(dan)白(bai) L（NFL）ELISA 檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)實(shi)(shi)(shi)驗(yan)(yan)(yan)原(yuan)(yuan)理   試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)采(cai)(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙抗(kang)體(ti)一(yi)(yi)(yi)(yi)步(bu)夾心(xin)法(fa)酶(mei)(mei)聯(lian)免(mian)(mian)疫吸附試(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)（ELISA）。往預先包被脂(zhi)肪酸(suan)結(jie)(jie)合蛋(dan)白(bai)1(FABP1)捕(bu)獲抗(kang)體(ti)的(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)(kong)(kong)(kong)(kong)中(zhong)，依(yi)(yi)次(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)體(ti)，經過(guo)(guo)(guo)(guo)(guo)(guo)溫(wen)(wen)育并(bing)徹底(di)洗(xi)滌。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)底(di)物(wu)(wu)TMB顯(xian)色(se)，TMB在(zai)(zai)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)物(wu)(wu)酶(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)下轉化(hua)成藍色(se)，并(bing)在(zai)(zai)酸(suan)的(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)下轉化(hua)成＊終(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)。顏色(se)的(de)(de)(de)(de)(de)(de)(de)(de)深淺(qian)和(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)脂(zhi)肪酸(suan)結(jie)(jie)合蛋(dan)白(bai)1(FABP1)呈正相(xiang)關(guan)(guan)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)450nm 波(bo)長下測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)吸光度(du)(du)(du)(du)(du)(du)（OD 值(zhi)(zhi)(zhi)），計(ji)算(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)。保(bao)存(cun)(cun)(cun)(cun)條(tiao)件(jian)及有(you)效期(qi)(qi)(qi)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)體(ti)外(wai)定(ding)量(liang)(liang)(liang)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)血(xue)清(qing)、血(xue)漿(jiang)、組(zu)織勻(yun)(yun)漿(jiang)及相(xiang)關(guan)(guan)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)脂(zhi)肪酸(suan)結(jie)(jie)合蛋(dan)白(bai)1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)含(han)量(liang)(liang)(liang)。有(you)效期(qi)(qi)(qi)：6個月保(bao)存(cun)(cun)(cun)(cun)條(tiao)件(jian)：2-8℃小(xiao)(xiao)(xiao)鼠神經絲蛋(dan)白(bai) L（NFL）ELISA 檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)一(yi)(yi)(yi)(yi)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)采(cai)(cai)集(ji)及保(bao)存(cun)(cun)(cun)(cun) 1.血(xue)清(qing)：全血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)請(qing)(qing)于(yu)(yu)室溫(wen)(wen)放(fang)置2小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)或(huo)(huo)4℃過(guo)(guo)(guo)(guo)(guo)(guo)一(yi)(yi)(yi)(yi)晚后(hou)(hou)(hou)(hou)(hou)于(yu)(yu)1000 x g離心(xin)20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，取上(shang)(shang)(shang)清(qing)即(ji)(ji)可(ke)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)-20℃或(huo)(huo)-80℃保(bao)存(cun)(cun)(cun)(cun)，但應(ying)(ying)(ying)(ying)(ying)避(bi)免(mian)(mian)反(fan)(fan)(fan)復(fu)(fu)凍(dong)融(rong)。 2.血(xue)漿(jiang)：可(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)肝素(su)(su)作(zuo)為(wei)(wei)(wei)抗(kang)凝劑(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)采(cai)(cai)集(ji)后(hou)(hou)(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)于(yu)(yu)2 - 8° C 1000 x g離心(xin)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，或(huo)(huo)將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)-20℃或(huo)(huo)-80℃保(bao)存(cun)(cun)(cun)(cun)，但應(ying)(ying)(ying)(ying)(ying)避(bi)免(mian)(mian)反(fan)(fan)(fan)復(fu)(fu)凍(dong)融(rong)。 3.細(xi)胞培(pei)養物(wu)(wu)上(shang)(shang)(shang)清(qing)或(huo)(huo)其(qi)它(ta)生(sheng)(sheng)(sheng)物(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)：1000 x g離心(xin)20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，取上(shang)(shang)(shang)清(qing)即(ji)(ji)可(ke)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)-20℃或(huo)(huo)-80℃保(bao)存(cun)(cun)(cun)(cun)，但應(ying)(ying)(ying)(ying)(ying)避(bi)免(mian)(mian)反(fan)(fan)(fan)復(fu)(fu)凍(dong)融(rong)。 注：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)溶(rong)血(xue)會(hui)影響檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)果(guo)(guo)，因此(ci)(ci)溶(rong)血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)不(bu)宜進(jin)行(xing)(xing)此(ci)(ci)項(xiang)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)。二、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)則   首先通(tong)過(guo)(guo)(guo)(guo)(guo)(guo)文獻檢(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)式(shi)了解待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)大致含(han)量(liang)(liang)(liang)，確定(ding)適當的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)。只(zhi)有(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)至(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)范圍(wei)內(nei)(nei)，檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)結(jie)(jie)果(guo)(guo)才是(shi)準(zhun)(zhun)確的(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)，應(ying)(ying)(ying)(ying)(ying)做好詳細(xi)的(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)錄。計(ji)算(suan)(suan)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)時(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)了“N”倍，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)應(ying)(ying)(ying)(ying)(ying)再(zai)乘以(yi)(yi)(yi)(yi)(yi)(yi)(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)則：2瓶(ping)，每(mei)(mei)瓶(ping)臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)至(zhi)1ml，蓋(gai)(gai)好后(hou)(hou)(hou)(hou)(hou)靜置10分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)上(shang)(shang)(shang)，然后(hou)(hou)(hou)(hou)(hou)反(fan)(fan)(fan)復(fu)(fu)顛(dian)倒/搓動(dong)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)助溶(rong)解，其(qi)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)300 pg/ml，做系(xi)列倍比(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)直(zhi)(zhi)(zhi)接作(zuo)為(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)0 pg/ml，臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。 如(ru)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取0.5ml（不(bu)要(yao)少(shao)于(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)含(han)0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)中(zhong)，混勻(yun)(yun)即(ji)(ji)可(ke)，其(qi)余濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)此(ci)(ci)類推(tui)。2.生(sheng)(sheng)(sheng)物(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)則： 臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)生(sheng)(sheng)(sheng)物(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)前(qian)根(gen)據(ju)預先計(ji)算(suan)(suan)好的(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)際配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)多(duo)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)10μl生(sheng)(sheng)(sheng)物(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)加(jia)(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)物(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混勻(yun)(yun)，在(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)一(yi)(yi)(yi)(yi)小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。3.辣(la)(la)(la)(la)根(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)物(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)素(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)則： 臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)辣(la)(la)(la)(la)根(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)物(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)素(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)前(qian)根(gen)據(ju)預先計(ji)算(suan)(suan)好的(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)際配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)多(duo)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)10μl辣(la)(la)(la)(la)根(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)物(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)素(su)(su)加(jia)(jia)(jia)(jia)(jia)(jia)990μl辣(la)(la)(la)(la)根(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)物(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)素(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混勻(yun)(yun)，在(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)一(yi)(yi)(yi)(yi)小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。 操作(zuo)步(bu)驟(zou)(zou) 實(shi)(shi)(shi)驗(yan)(yan)(yan)開(kai)始(shi)前(qian)，請(qing)(qing)提(ti)前(qian)配(pei)(pei)(pei)置好所有(you)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)或(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)，均(jun)需(xu)混勻(yun)(yun)，混勻(yun)(yun)時(shi)(shi)(shi)(shi)盡(jin)量(liang)(liang)(liang)避(bi)免(mian)(mian)起泡(pao)。每(mei)(mei)次(ci)(ci)(ci)(ci)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)(ying)該做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)。如(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)過(guo)(guo)(guo)(guo)(guo)(guo)高(gao)時(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)進(jin)行(xing)(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)(yi)(yi)(yi)使(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)范圍(wei)。 小(xiao)(xiao)(xiao)鼠脂(zhi)肪酸(suan)結(jie)(jie)合蛋(dan)白(bai)1(FABP1)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)三(san)、操作(zuo)步(bu)驟(zou)(zou)1.加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)別(bie)設空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)、待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)。空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注意不(bu)要(yao)有(you)氣(qi)泡(pao)，加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)于(yu)(yu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)底(di)部，盡(jin)量(liang)(liang)(liang)不(bu)觸(chu)及孔(kong)(kong)(kong)(kong)(kong)壁(bi)，輕(qing)輕(qing)晃動(dong)混勻(yun)(yun)，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)蓋(gai)(gai)或(huo)(huo)覆膜(mo)，37℃反(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。 為(wei)(wei)(wei)保(bao)證實(shi)(shi)(shi)驗(yan)(yan)(yan)結(jie)(jie)果(guo)(guo)有(you)效性(xing)(xing)，每(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)請(qing)(qing)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)新(xin)的(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。2.棄(qi)(qi)去(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，甩(shuai)(shuai)干(gan)，不(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)滌。每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)(sheng)物(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)(sheng)(sheng)物(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)加(jia)(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)物(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混勻(yun)(yun)，在(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)一(yi)(yi)(yi)(yi)小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。 3.溫(wen)(wen)育60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)(hou)(hou)(hou)，棄(qi)(qi)去(qu)(qu)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，甩(shuai)(shuai)干(gan)，洗(xi)板(ban)(ban)(ban)(ban)3次(ci)(ci)(ci)(ci)，每(mei)(mei)次(ci)(ci)(ci)(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，350μl/每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)干(gan)。 4.每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)辣(la)(la)(la)(la)根(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)物(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)素(su)(su)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同(tong)生(sheng)(sheng)(sheng)物(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。 5.溫(wen)(wen)育60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)(hou)(hou)(hou)，棄(qi)(qi)去(qu)(qu)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，甩(shuai)(shuai)干(gan)，洗(xi)板(ban)(ban)(ban)(ban)5次(ci)(ci)(ci)(ci)，每(mei)(mei)次(ci)(ci)(ci)(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，350μl/每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)干(gan)。 6.依(yi)(yi)序每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)底(di)物(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)光顯(xian)色(se)（30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)，此(ci)(ci)時(shi)(shi)(shi)(shi)肉眼可(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)前(qian)3-4孔(kong)(kong)(kong)(kong)(kong)有(you)明(ming)顯(xian)的(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)(du)(du)(du)藍色(se)，后(hou)(hou)(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)梯度(du)(du)(du)(du)(du)(du)不(bu)明(ming)顯(xian)，即(ji)(ji)可(ke)終(zhong)(zhong)(zhong)(zhong)止）。 7.依(yi)(yi)序每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)(zhong)(zhong)止溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)(zhong)(zhong)止反(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)（此(ci)(ci)時(shi)(shi)(shi)(shi)藍色(se)立(li)轉黃色(se)）。終(zhong)(zhong)(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)順序應(ying)(ying)(ying)(ying)(ying)盡(jin)量(liang)(liang)(liang)與(yu)底(di)物(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)順序相(xiang)同(tong)。為(wei)(wei)(wei)了保(bao)證實(shi)(shi)(shi)驗(yan)(yan)(yan)結(jie)(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)確性(xing)(xing)，底(di)物(wu)(wu)反(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)間到(dao)(dao)后(hou)(hou)(hou)(hou)(hou)應(ying)(ying)(ying)(ying)(ying)盡(jin)快加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)終(zhong)(zhong)(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)聯(lian)儀在(zai)(zai)450nm波(bo)長依(yi)(yi)序測(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)各孔(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)光密(mi)度(du)(du)(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)）。 在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)內(nei)(nei)進(jin)行(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)。 四、試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)小(xiao)(xiao)(xiao)技(ji)巧1. 用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)初(chu)次(ci)(ci)(ci)(ci)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)時(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)(ying)將各種試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)管(guan)離心(xin)數(shu)(shu)(shu)(shu)分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，以(yi)(yi)(yi)(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)集(ji)中(zhong)到(dao)(dao)管(guan)底(di)。2. 每(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)留(liu)一(yi)(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)作(zuo)為(wei)(wei)(wei)空(kong)白(bai)調零孔(kong)(kong)(kong)(kong)(kong)，該孔(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)(jia)(jia)(jia)(jia)任何(he)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，只(zhi)是(shi)**加(jia)(jia)(jia)(jia)(jia)(jia)底(di)物(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)及2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)時(shi)(shi)(shi)(shi)先用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)此(ci)(ci)孔(kong)(kong)(kong)(kong)(kong)調OD值(zhi)(zhi)(zhi)至(zhi)零。 3. 為(wei)(wei)(wei)防止樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發(fa)，試(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)時(shi)(shi)(shi)(shi)將反(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)板(ban)(ban)(ban)(ban)放(fang)于(yu)(yu)鋪有(you)濕(shi)布的(de)(de)(de)(de)(de)(de)(de)(de)密(mi)閉(bi)盒(he)(he)(he)(he)內(nei)(nei)，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)蓋(gai)(gai)或(huo)(huo)覆膜(mo)。 4. 未使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)或(huo)(huo)者試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，請(qing)(qing)于(yu)(yu)2-8℃保(bao)存(cun)(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)(la)(la)(la)根(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)物(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)素(su)(su)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)請(qing)(qing)依(yi)(yi)據(ju)所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)配(pei)(pei)(pei)置使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)勿重復(fu)(fu)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)抗(kang)體(ti)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)、辣(la)(la)(la)(la)根(gen)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)物(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)親(qin)和(he)(he)素(su)(su)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5. 建議檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)均(jun)設雙孔(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)，以(yi)(yi)(yi)(yi)(yi)(yi)(yi)保(bao)證檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)確性(xing)(xing)。 洗(xi)板(ban)(ban)(ban)(ban)方(fang)(fang)法(fa) 手工(gong)(gong)洗(xi)板(ban)(ban)(ban)(ban)方(fang)(fang)法(fa)：吸去(qu)(qu)（不(bu)可(ke)觸(chu)及板(ban)(ban)(ban)(ban)壁(bi)）或(huo)(huo)甩(shuai)(shuai)掉酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)內(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)；在(zai)(zai)實(shi)(shi)(shi)驗(yan)(yan)(yan)臺(tai)上(shang)(shang)(shang)鋪墊幾層吸水(shui)紙，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)朝下用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)力拍幾次(ci)(ci)(ci)(ci)；將推(tui)薦的(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌緩沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)至(zhi)少(shao)0.3ml注入(ru)(ru)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)，浸泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。根(gen)據(ju)需(xu)要(yao)，重復(fu)(fu)此(ci)(ci)過(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)數(shu)(shu)(shu)(shu)次(ci)(ci)(ci)(ci)。 自動(dong)洗(xi)板(ban)(ban)(ban)(ban)：如(ru)果(guo)(guo)有(you)自動(dong)洗(xi)板(ban)(ban)(ban)(ban)機，應(ying)(ying)(ying)(ying)(ying)在(zai)(zai)熟(shu)練使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)(hou)(hou)再(zai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)到(dao)(dao)正式(shi)實(shi)(shi)(shi)驗(yan)(yan)(yan)過(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)。 計(ji)算(suan)(suan) 以(yi)(yi)(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對(dui)數(shu)(shu)(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通(tong)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)半對(dui)數(shu)(shu)(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)繪出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)，根(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)查出(chu)(chu)(chu)相(xiang)應(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)；再(zai)乘以(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)；或(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)算(suan)(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)(zhi)(zhi)線(xian)回(hui)歸(gui)方(fang)(fang)程(cheng)(cheng)(cheng)(cheng)式(shi)，將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)(ru)方(fang)(fang)程(cheng)(cheng)(cheng)(cheng)式(shi)，計(ji)算(suan)(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)，再(zai)乘以(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)，即(ji)(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)。 五、技(ji)術小(xiao)(xiao)(xiao)提(ti)示1.當混合蛋(dan)白(bai)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)盡(jin)量(liang)(liang)(liang)輕(qing)緩，避(bi)免(mian)(mian)起泡(pao)。2. 洗(xi)滌過(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)非(fei)常(chang)重要(yao)，不(bu)充分(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌易造成假(jia)陽性(xing)(xing)。 3. 一(yi)(yi)(yi)(yi)次(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)間控(kong)制(zhi)(zhi)(zhi)(zhi)(zhi)在(zai)(zai)5分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)數(shu)(shu)(shu)(shu)量(liang)(liang)(liang)多(duo)，推(tui)薦使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)排槍(qiang)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。 4. 請(qing)(qing)每(mei)(mei)次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi)(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)，做復(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)。 5. 如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)質含(han)量(liang)(liang)(liang)過(guo)(guo)(guo)(guo)(guo)(guo)高(gao)，請(qing)(qing)先稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)再(zai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)，計(ji)算(suan)(suan)時(shi)(shi)(shi)(shi)請(qing)(qing)乘以(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)。 6. 在(zai)(zai)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)，請(qing)(qing)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)相(xiang)應(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，不(bu)能混淆。 7. 底(di)物(wu)(wu)請(qing)(qing)避(bi)光保(bao)存(cun)(cun)(cun)(cun)。 8. 不(bu)要(yao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)其(qi)它(ta)生(sheng)(sheng)(sheng)產廠(chang)家的(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)替換試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)。 六、注意事項(xiang)1.收(shou)集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)前(qian)必須清(qing)楚要(yao)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)成份是(shi)否足夠穩定(ding)。對(dui)收(shou)集(ji)后(hou)(hou)(hou)(hou)(hou)當天進(jin)行(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)，儲存(cun)(cun)(cun)(cun)在(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)有(you)特殊(shu)原(yuan)(yuan)因需(xu)要(yao)周期(qi)(qi)(qi)收(shou)集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)， 將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)及時(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)裝(zhuang)后(hou)(hou)(hou)(hou)(hou)放(fang)在(zai)(zai)-20 ℃或(huo)(huo)-70 ℃條(tiao)件(jian)下保(bao)存(cun)(cun)(cun)(cun)。避(bi)免(mian)(mian)反(fan)(fan)(fan)復(fu)(fu)凍(dong)融(rong)。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)2 -8 ℃可(ke)保(bao)存(cun)(cun)(cun)(cun)48 小(xiao)(xiao)(xiao)時(shi)(shi)(shi)(shi)，-20 ℃可(ke)保(bao)存(cun)(cun)(cun)(cun)1 個月。-70度(du)(du)(du)(du)(du)(du)可(ke)保(bao)存(cun)(cun)(cun)(cun)6 個月。3.部分(fen)(fen)(fen)(fen)激素(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)添(tian)加(jia)(jia)(jia)(jia)(jia)(jia)抑肽酶(mei)(mei)。 4.計(ji)算(suan)(suan)方(fang)(fang)法(fa)：以(yi)(yi)(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)繪出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)，根(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)查出(chu)(chu)(chu)相(xiang)應(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)；再(zai)乘以(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)；或(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)算(suan)(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)(qu)(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)(zhi)(zhi)線(xian)回(hui)歸(gui)方(fang)(fang)程(cheng)(cheng)(cheng)(cheng)式(shi)，將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)(ru)方(fang)(fang)程(cheng)(cheng)(cheng)(cheng)式(shi)，計(ji)算(suan)(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)，再(zai)乘以(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)(shu)，即(ji)(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)。 七、試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)性(xing)(xing)能1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)性(xing)(xing)回(hui)歸(gui)與(yu)預期(qi)(qi)(qi)濃(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)相(xiang)關(guan)(guan)系(xi)數(shu)(shu)(shu)(shu)R值(zhi)(zhi)(zhi)為(wei)(wei)(wei)0.92以(yi)(yi)(yi)(yi)(yi)(yi)(yi)上(shang)(shang)(shang)。2.批內(nei)(nei)與(yu)批見應(ying)(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)別(bie)小(xiao)(xiao)(xiao)于(yu)(yu)9%和(he)(he)15% 八、保(bao)存(cun)(cun)(cun)(cun)條(tiao)件(jian)及有(you)效期(qi)(qi)(qi)1.試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)保(bao)存(cun)(cun)(cun)(cun)：2-8℃。 2．有(you)效期(qi)(qi)(qi)：6個月

 小(xiao)(xiao)鼠(shu)(shu)脂肪酸結(jie)(jie)(jie)(jie)合蛋(dan)(dan)白(bai)(bai)1(FABP1)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)原(yuan)理   試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)采(cai)用(yong)(yong)(yong)(yong)雙(shuang)(shuang)抗(kang)(kang)體(ti)(ti)一(yi)步夾心(xin)(xin)法(fa)酶(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)疫(yi)吸附試(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)（ELISA）。往預(yu)先(xian)包(bao)被(bei)脂肪酸結(jie)(jie)(jie)(jie)合蛋(dan)(dan)白(bai)(bai)1(FABP1)捕獲抗(kang)(kang)體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包(bao)被(bei)微孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，依次(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)抗(kang)(kang)體(ti)(ti)，經(jing)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)溫(wen)育并(bing)徹底(di)洗(xi)滌。用(yong)(yong)(yong)(yong)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯(xian)色(se)(se)(se)，TMB在(zai)(zai)(zai)(zai)(zai)(zai)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化下(xia)(xia)轉化成藍(lan)色(se)(se)(se)，并(bing)在(zai)(zai)(zai)(zai)(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)下(xia)(xia)轉化成＊終的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)(se)(se)。顏色(se)(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂肪酸結(jie)(jie)(jie)(jie)合蛋(dan)(dan)白(bai)(bai)1(FABP1)呈(cheng)正相(xiang)(xiang)(xiang)關。用(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)在(zai)(zai)(zai)(zai)(zai)(zai)450nm 波長下(xia)(xia)測(ce)(ce)(ce)定(ding)吸光度(du)(du)(du)（OD 值(zhi)(zhi)(zhi)），計(ji)(ji)算樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)度(du)(du)(du)。保(bao)(bao)存(cun)(cun)(cun)條件(jian)及有(you)(you)(you)(you)(you)效(xiao)期(qi)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)用(yong)(yong)(yong)(yong)于(yu)體(ti)(ti)外定(ding)量(liang)(liang)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)血清(qing)、血漿(jiang)、組(zu)織勻(yun)(yun)(yun)漿(jiang)及相(xiang)(xiang)(xiang)關液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)脂肪酸結(jie)(jie)(jie)(jie)合蛋(dan)(dan)白(bai)(bai)1(FABP1)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)。有(you)(you)(you)(you)(you)效(xiao)期(qi)：6個(ge)月(yue)保(bao)(bao)存(cun)(cun)(cun)條件(jian)：2-8℃小(xiao)(xiao)鼠(shu)(shu)脂肪酸結(jie)(jie)(jie)(jie)合蛋(dan)(dan)白(bai)(bai)1(FABP1)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)一(yi)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集(ji)及保(bao)(bao)存(cun)(cun)(cun) 1.血清(qing)：全血標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)請(qing)(qing)(qing)(qing)于(yu)室溫(wen)放置(zhi)2小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)(huo)(huo)(huo)4℃過(guo)(guo)(guo)(guo)(guo)(guo)(guo)一(yi)晚(wan)后(hou)(hou)(hou)于(yu)1000 x g離(li)心(xin)(xin)20分(fen)鐘(zhong)(zhong)，取上(shang)(shang)(shang)(shang)清(qing)即(ji)可(ke)(ke)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)放于(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)(cun)(cun)，但(dan)應(ying)(ying)避(bi)免(mian)(mian)反復(fu)(fu)(fu)凍(dong)(dong)融。 2.血漿(jiang)：可(ke)(ke)用(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)(huo)(huo)肝(gan)素(su)作(zuo)(zuo)為(wei)(wei)(wei)抗(kang)(kang)凝劑(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)采(cai)集(ji)后(hou)(hou)(hou)30分(fen)鐘(zhong)(zhong)內(nei)(nei)于(yu)2 - 8° C 1000 x g離(li)心(xin)(xin)15分(fen)鐘(zhong)(zhong)，或(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)放于(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)(cun)(cun)，但(dan)應(ying)(ying)避(bi)免(mian)(mian)反復(fu)(fu)(fu)凍(dong)(dong)融。 3.細胞培養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)(shang)清(qing)或(huo)(huo)(huo)(huo)(huo)(huo)其它生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)：1000 x g離(li)心(xin)(xin)20分(fen)鐘(zhong)(zhong)，取上(shang)(shang)(shang)(shang)清(qing)即(ji)可(ke)(ke)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)放于(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)(cun)(cun)，但(dan)應(ying)(ying)避(bi)免(mian)(mian)反復(fu)(fu)(fu)凍(dong)(dong)融。 注(zhu)(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)溶(rong)血會影響檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)結(jie)(jie)(jie)(jie)果(guo)，因此(ci)(ci)溶(rong)血標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)不(bu)宜進(jin)行此(ci)(ci)項檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)。二、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)原(yuan)則(ze)   首先(xian)通過(guo)(guo)(guo)(guo)(guo)(guo)(guo)文獻檢(jian)(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)(fang)式(shi)了解(jie)待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致(zhi)含量(liang)(liang)，確(que)定(ding)適(shi)當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)倍(bei)(bei)數(shu)。只有(you)(you)(you)(you)(you)稀(xi)(xi)(xi)釋(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍內(nei)(nei)，檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)(jie)(jie)(jie)果(guo)才是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)釋(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)中(zhong)，應(ying)(ying)做(zuo)(zuo)好(hao)詳(xiang)細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)(ji)錄。計(ji)(ji)算濃(nong)度(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)釋(shi)(shi)了“N”倍(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)應(ying)(ying)再(zai)乘以(yi)(yi)(yi)(yi)(yi)“N”。 1.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)原(yuan)則(ze)：2瓶，每(mei)(mei)瓶臨用(yong)(yong)(yong)(yong)前以(yi)(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)釋(shi)(shi)至1ml，蓋(gai)好(hao)后(hou)(hou)(hou)靜置(zhi)10分(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)上(shang)(shang)(shang)(shang)，然后(hou)(hou)(hou)反復(fu)(fu)(fu)顛倒/搓動以(yi)(yi)(yi)(yi)(yi)助溶(rong)解(jie)，其濃(nong)度(du)(du)(du)為(wei)(wei)(wei)300 pg/ml，做(zuo)(zuo)系列倍(bei)(bei)比稀(xi)(xi)(xi)釋(shi)(shi)后(hou)(hou)(hou)，分(fen)別(bie)稀(xi)(xi)(xi)釋(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)直接作(zuo)(zuo)為(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)度(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)前15分(fen)鐘(zhong)(zhong)內(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。 如(ru)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)：取0.5ml（不(bu)要(yao)(yao)少(shao)(shao)于(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)含0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)中(zhong)，混(hun)勻(yun)(yun)(yun)即(ji)可(ke)(ke)，其余濃(nong)度(du)(du)(du)以(yi)(yi)(yi)(yi)(yi)此(ci)(ci)類推(tui)。2.生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)原(yuan)則(ze)： 臨用(yong)(yong)(yong)(yong)前以(yi)(yi)(yi)(yi)(yi)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)釋(shi)(shi)，稀(xi)(xi)(xi)釋(shi)(shi)前根(gen)(gen)(gen)(gen)據(ju)(ju)預(yu)先(xian)計(ji)(ji)算好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)次(ci)(ci)(ci)實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)所(suo)(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)(shi)(shi)(shi)際配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)多(duo)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)10μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)勻(yun)(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)前一(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。3.辣(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和(he)(he)素(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)原(yuan)則(ze)： 臨用(yong)(yong)(yong)(yong)前以(yi)(yi)(yi)(yi)(yi)辣(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和(he)(he)素(su)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)釋(shi)(shi)，稀(xi)(xi)(xi)釋(shi)(shi)前根(gen)(gen)(gen)(gen)據(ju)(ju)預(yu)先(xian)計(ji)(ji)算好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)次(ci)(ci)(ci)實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)所(suo)(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)(shi)(shi)(shi)際配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)多(duo)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)10μl辣(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和(he)(he)素(su)加(jia)(jia)(jia)(jia)(jia)(jia)990μl辣(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和(he)(he)素(su)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)勻(yun)(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)前一(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。 操(cao)作(zuo)(zuo)步驟 實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)開始前，請(qing)(qing)(qing)(qing)提前配(pei)(pei)(pei)置(zhi)好(hao)所(suo)(suo)有(you)(you)(you)(you)(you)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)或(huo)(huo)(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)，均需(xu)混(hun)勻(yun)(yun)(yun)，混(hun)勻(yun)(yun)(yun)時(shi)(shi)(shi)(shi)(shi)(shi)盡量(liang)(liang)避(bi)免(mian)(mian)起泡。每(mei)(mei)次(ci)(ci)(ci)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)都應(ying)(ying)該(gai)做(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)。如(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)度(du)(du)(du)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)高(gao)時(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)進(jin)行稀(xi)(xi)(xi)釋(shi)(shi)，以(yi)(yi)(yi)(yi)(yi)使(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)符(fu)合試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)范圍。 小(xiao)(xiao)鼠(shu)(shu)脂肪酸結(jie)(jie)(jie)(jie)合蛋(dan)(dan)白(bai)(bai)1(FABP1)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)三、操(cao)作(zuo)(zuo)步驟1.加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)別(bie)設空白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)(kong)、待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)。空白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)(kong)分(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)(huo)(huo)待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)(zhu)意不(bu)要(yao)(yao)有(you)(you)(you)(you)(you)氣泡，加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)于(yu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)底(di)部(bu)，盡量(liang)(liang)不(bu)觸(chu)及孔(kong)(kong)(kong)(kong)(kong)(kong)壁(bi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)晃動混(hun)勻(yun)(yun)(yun)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)(shang)蓋(gai)或(huo)(huo)(huo)(huo)(huo)(huo)覆膜，37℃反應(ying)(ying)120分(fen)鐘(zhong)(zhong)。 為(wei)(wei)(wei)保(bao)(bao)證(zheng)實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)結(jie)(jie)(jie)(jie)果(guo)有(you)(you)(you)(you)(you)效(xiao)性，每(mei)(mei)次(ci)(ci)(ci)實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)請(qing)(qing)(qing)(qing)使(shi)(shi)用(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)。2.棄(qi)去(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，甩(shuai)(shuai)(shuai)(shuai)干(gan)(gan)，不(bu)用(yong)(yong)(yong)(yong)洗(xi)滌。每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)輕(qing)(qing)(qing)混(hun)勻(yun)(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)前一(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)鐘(zhong)(zhong)。 3.溫(wen)育60分(fen)鐘(zhong)(zhong)后(hou)(hou)(hou)，棄(qi)去(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，甩(shuai)(shuai)(shuai)(shuai)干(gan)(gan)，洗(xi)板(ban)(ban)(ban)3次(ci)(ci)(ci)，每(mei)(mei)次(ci)(ci)(ci)浸泡1-2分(fen)鐘(zhong)(zhong)，350μl/每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)(shuai)(shuai)干(gan)(gan)。 4.每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)辣(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和(he)(he)素(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)（同生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)鐘(zhong)(zhong)。 5.溫(wen)育60分(fen)鐘(zhong)(zhong)后(hou)(hou)(hou)，棄(qi)去(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，甩(shuai)(shuai)(shuai)(shuai)干(gan)(gan)，洗(xi)板(ban)(ban)(ban)5次(ci)(ci)(ci)，每(mei)(mei)次(ci)(ci)(ci)浸泡1-2分(fen)鐘(zhong)(zhong)，350μl/每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)(shuai)(shuai)干(gan)(gan)。 6.依序(xu)每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)光顯(xian)色(se)(se)(se)（30分(fen)鐘(zhong)(zhong)內(nei)(nei)，此(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)肉眼可(ke)(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前3-4孔(kong)(kong)(kong)(kong)(kong)(kong)有(you)(you)(you)(you)(you)明顯(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)藍(lan)色(se)(se)(se)，后(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)梯度(du)(du)(du)不(bu)明顯(xian)，即(ji)可(ke)(ke)終止(zhi)(zhi)）。 7.依序(xu)每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)終止(zhi)(zhi)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，終止(zhi)(zhi)反應(ying)(ying)（此(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)藍(lan)色(se)(se)(se)立轉黃色(se)(se)(se)）。終止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)順序(xu)應(ying)(ying)盡量(liang)(liang)與(yu)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)順序(xu)相(xiang)(xiang)(xiang)同。為(wei)(wei)(wei)了保(bao)(bao)證(zheng)實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)結(jie)(jie)(jie)(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性，底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反應(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)間到(dao)(dao)后(hou)(hou)(hou)應(ying)(ying)盡快加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)終止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)。 8.用(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)聯(lian)儀(yi)在(zai)(zai)(zai)(zai)(zai)(zai)450nm波長依序(xu)測(ce)(ce)(ce)量(liang)(liang)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光密度(du)(du)(du)（OD值(zhi)(zhi)(zhi)）。 在(zai)(zai)(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)終止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)內(nei)(nei)進(jin)行檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)。 四、試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)使(shi)(shi)用(yong)(yong)(yong)(yong)小(xiao)(xiao)技(ji)巧1. 用(yong)(yong)(yong)(yong)戶(hu)在(zai)(zai)(zai)(zai)(zai)(zai)初次(ci)(ci)(ci)使(shi)(shi)用(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)時(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)將(jiang)各(ge)種試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)管(guan)離(li)心(xin)(xin)數(shu)分(fen)鐘(zhong)(zhong)，以(yi)(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)集(ji)中(zhong)到(dao)(dao)管(guan)底(di)。2. 每(mei)(mei)次(ci)(ci)(ci)實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)留一(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)為(wei)(wei)(wei)空白(bai)(bai)調(diao)零孔(kong)(kong)(kong)(kong)(kong)(kong)，該(gai)孔(kong)(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)(jia)(jia)(jia)(jia)任(ren)何試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，只是**加(jia)(jia)(jia)(jia)(jia)(jia)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)及2N H2SO4。測(ce)(ce)(ce)量(liang)(liang)時(shi)(shi)(shi)(shi)(shi)(shi)先(xian)用(yong)(yong)(yong)(yong)此(ci)(ci)孔(kong)(kong)(kong)(kong)(kong)(kong)調(diao)OD值(zhi)(zhi)(zhi)至零。 3. 為(wei)(wei)(wei)防止(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)時(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)反應(ying)(ying)板(ban)(ban)(ban)放于(yu)鋪有(you)(you)(you)(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)(he)(he)內(nei)(nei)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)(shang)蓋(gai)或(huo)(huo)(huo)(huo)(huo)(huo)覆膜。 4. 未使(shi)(shi)用(yong)(yong)(yong)(yong)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)或(huo)(huo)(huo)(huo)(huo)(huo)者(zhe)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，請(qing)(qing)(qing)(qing)于(yu)2-8℃保(bao)(bao)存(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和(he)(he)素(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)請(qing)(qing)(qing)(qing)依據(ju)(ju)所(suo)(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)配(pei)(pei)(pei)置(zhi)使(shi)(shi)用(yong)(yong)(yong)(yong)。請(qing)(qing)(qing)(qing)勿重復(fu)(fu)(fu)使(shi)(shi)用(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)釋(shi)(shi)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)(huo)(huo)、辣(la)(la)根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)親(qin)(qin)和(he)(he)素(su)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)。 5. 建議檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)均設雙(shuang)(shuang)孔(kong)(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)定(ding)，以(yi)(yi)(yi)(yi)(yi)保(bao)(bao)證(zheng)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)結(jie)(jie)(jie)(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性。 洗(xi)板(ban)(ban)(ban)方(fang)(fang)(fang)法(fa) 手(shou)工(gong)(gong)(gong)洗(xi)板(ban)(ban)(ban)方(fang)(fang)(fang)法(fa)：吸去(qu)（不(bu)可(ke)(ke)觸(chu)及板(ban)(ban)(ban)壁(bi)）或(huo)(huo)(huo)(huo)(huo)(huo)甩(shuai)(shuai)(shuai)(shuai)掉酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)內(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)；在(zai)(zai)(zai)(zai)(zai)(zai)實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)臺上(shang)(shang)(shang)(shang)鋪墊幾層(ceng)吸水紙，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)朝下(xia)(xia)用(yong)(yong)(yong)(yong)力拍幾次(ci)(ci)(ci)；將(jiang)推(tui)薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌緩(huan)沖液(ye)(ye)(ye)(ye)(ye)(ye)至少(shao)(shao)0.3ml注(zhu)(zhu)入(ru)孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)，浸泡1-2分(fen)鐘(zhong)(zhong)。根(gen)(gen)(gen)(gen)據(ju)(ju)需(xu)要(yao)(yao)，重復(fu)(fu)(fu)此(ci)(ci)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)數(shu)次(ci)(ci)(ci)。 自(zi)動洗(xi)板(ban)(ban)(ban)：如(ru)果(guo)有(you)(you)(you)(you)(you)自(zi)動洗(xi)板(ban)(ban)(ban)機，應(ying)(ying)在(zai)(zai)(zai)(zai)(zai)(zai)熟(shu)練使(shi)(shi)用(yong)(yong)(yong)(yong)后(hou)(hou)(hou)再(zai)用(yong)(yong)(yong)(yong)到(dao)(dao)正式(shi)實(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)中(zhong)。 計(ji)(ji)算 以(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)為(wei)(wei)(wei)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對(dui)數(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)(zai)(zai)(zai)半對(dui)數(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)，根(gen)(gen)(gen)(gen)據(ju)(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)查出(chu)相(xiang)(xiang)(xiang)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)；再(zai)乘以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)倍(bei)(bei)數(shu)；或(huo)(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)(ji)算出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)回(hui)歸(gui)方(fang)(fang)(fang)程(cheng)(cheng)式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)方(fang)(fang)(fang)程(cheng)(cheng)式(shi)，計(ji)(ji)算出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)度(du)(du)(du)，再(zai)乘以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)倍(bei)(bei)數(shu)，即(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)(shi)(shi)(shi)際濃(nong)度(du)(du)(du)。 五、技(ji)術小(xiao)(xiao)提示1.當混(hun)合蛋(dan)(dan)白(bai)(bai)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)盡量(liang)(liang)輕(qing)(qing)(qing)緩(huan)，避(bi)免(mian)(mian)起泡。2. 洗(xi)滌過(guo)(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)非常(chang)重要(yao)(yao)，不(bu)充分(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌易造成假陽性。 3. 一(yi)次(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)間控(kong)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)在(zai)(zai)(zai)(zai)(zai)(zai)5分(fen)鐘(zhong)(zhong)內(nei)(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)數(shu)量(liang)(liang)多(duo)，推(tui)薦使(shi)(shi)用(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)。 4. 請(qing)(qing)(qing)(qing)每(mei)(mei)次(ci)(ci)(ci)測(ce)(ce)(ce)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)(shi)(shi)(shi)做(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)，做(zuo)(zuo)復(fu)(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)。 5. 如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)待測(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)(liang)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)高(gao)，請(qing)(qing)(qing)(qing)先(xian)稀(xi)(xi)(xi)釋(shi)(shi)后(hou)(hou)(hou)再(zai)測(ce)(ce)(ce)定(ding)，計(ji)(ji)算時(shi)(shi)(shi)(shi)(shi)(shi)請(qing)(qing)(qing)(qing)乘以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)倍(bei)(bei)數(shu)。 6. 在(zai)(zai)(zai)(zai)(zai)(zai)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)(qing)(qing)(qing)以(yi)(yi)(yi)(yi)(yi)相(xiang)(xiang)(xiang)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，不(bu)能(neng)混(hun)淆。 7. 底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)(qing)(qing)避(bi)光保(bao)(bao)存(cun)(cun)(cun)。 8. 不(bu)要(yao)(yao)用(yong)(yong)(yong)(yong)其它生(sheng)(sheng)產(chan)廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)替換試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)。 六、注(zhu)(zhu)意事(shi)項1.收(shou)(shou)集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)前必須清(qing)楚要(yao)(yao)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成份是否足夠穩(wen)定(ding)。對(dui)收(shou)(shou)集(ji)后(hou)(hou)(hou)當天(tian)進(jin)行檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)，儲存(cun)(cun)(cun)在(zai)(zai)(zai)(zai)(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)，如(ru)有(you)(you)(you)(you)(you)特殊原(yuan)因需(xu)要(yao)(yao)周期(qi)收(shou)(shou)集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)， 將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)及時(shi)(shi)(shi)(shi)(shi)(shi)分(fen)裝后(hou)(hou)(hou)放在(zai)(zai)(zai)(zai)(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)(huo)(huo)-70 ℃條件(jian)下(xia)(xia)保(bao)(bao)存(cun)(cun)(cun)。避(bi)免(mian)(mian)反復(fu)(fu)(fu)凍(dong)(dong)融。2.標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)保(bao)(bao)存(cun)(cun)(cun)48 小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)保(bao)(bao)存(cun)(cun)(cun)1 個(ge)月(yue)。-70度(du)(du)(du)可(ke)(ke)保(bao)(bao)存(cun)(cun)(cun)6 個(ge)月(yue)。3.部(bu)分(fen)激素(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)添加(jia)(jia)(jia)(jia)(jia)(jia)抑肽酶(mei)(mei)(mei)(mei)。 4.計(ji)(ji)算方(fang)(fang)(fang)法(fa)：以(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)為(wei)(wei)(wei)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)(zai)(zai)(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)，根(gen)(gen)(gen)(gen)據(ju)(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)查出(chu)相(xiang)(xiang)(xiang)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)；再(zai)乘以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)倍(bei)(bei)數(shu)；或(huo)(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)(ji)算出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)回(hui)歸(gui)方(fang)(fang)(fang)程(cheng)(cheng)式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)方(fang)(fang)(fang)程(cheng)(cheng)式(shi)，計(ji)(ji)算出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)度(du)(du)(du)，再(zai)乘以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)倍(bei)(bei)數(shu)，即(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)(shi)(shi)(shi)際濃(nong)度(du)(du)(du)。 七、試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)性能(neng)1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)性回(hui)歸(gui)與(yu)預(yu)期(qi)濃(nong)度(du)(du)(du)相(xiang)(xiang)(xiang)關系數(shu)R值(zhi)(zhi)(zhi)為(wei)(wei)(wei)0.92以(yi)(yi)(yi)(yi)(yi)上(shang)(shang)(shang)(shang)。2.批內(nei)(nei)與(yu)批見應(ying)(ying)分(fen)別(bie)小(xiao)(xiao)于(yu)9%和(he)(he)15% 八、保(bao)(bao)存(cun)(cun)(cun)條件(jian)及有(you)(you)(you)(you)(you)效(xiao)期(qi)1.試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)保(bao)(bao)存(cun)(cun)(cun)：2-8℃。 2．有(you)(you)(you)(you)(you)效(xiao)期(qi)：6個(ge)月(yue)

小(xiao)(xiao)鼠纖連(lian)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)1(FN-1)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)技(ji)術(shu)流(liu)程  雙抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)夾(jia)心(xin)(xin)(xin)(xin)法(fa)(檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)未(wei)(wei)知(zhi)抗(kang)(kang)(kang)(kang)(kang)原(yuan)(yuan))間(jian)(jian)接法(fa)(檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)未(wei)(wei)知(zhi)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti))1、用(yong)(yong)(yong)(yong)緩(huan)(huan)沖液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)將(jiang)(jiang)(jiang)(jiang)(jiang)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)至(zhi)(zhi)1-10ug/ml。在(zai)(zai)(zai)(zai)反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)0.1ml，4℃ 過夜(ye)。次(ci)(ci)(ci)(ci)(ci)日(ri)，棄去(qu)(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)(nei)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，用(yong)(yong)(yong)(yong)洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)緩(huan)(huan)沖液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)3次(ci)(ci)(ci)(ci)(ci)。2、加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：加(jia)(jia)(jia)(jia)(jia)(jia)(jia)一(yi)(yi)(yi)定(ding)(ding)(ding)(ding)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)待(dai)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)0.05ml于(yu)(yu)(yu)(yu)(yu)(yu)(yu)上(shang)(shang)(shang)(shang)(shang)述已(yi)(yi)包(bao)被(bei)(bei)(bei)之反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，置(zhi)37℃ 孵(fu)(fu)(fu)育1小(xiao)(xiao)時(shi)。然后(hou)(hou)(hou)(hou)(hou)洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)(同時(shi)做(zuo)(zuo)空(kong)(kong)(kong)白(bai)(bai)(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，陰(yin)(yin)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)及(ji)(ji)(ji)(ji)(ji)陽(yang)(yang)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong))。3、加(jia)(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)：于(yu)(yu)(yu)(yu)(yu)(yu)(yu)各反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)新(xin)鮮(xian)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(經滴定(ding)(ding)(ding)(ding)后(hou)(hou)(hou)(hou)(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)度(du)(du)(du)(du))0.05ml。37℃ 孵(fu)(fu)(fu)育0.5～1小(xiao)(xiao)時(shi)，洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)。4、加(jia)(jia)(jia)(jia)(jia)(jia)(jia)底物(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)顯(xian)(xian)色(se)(se)(se)(se)(se)：于(yu)(yu)(yu)(yu)(yu)(yu)(yu)各反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)臨時(shi)配(pei)制(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)TMB底物(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)0.1ml，37℃ 10～30分(fen)(fen)(fen)(fen)鐘(zhong)。5、終(zhong)止反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)：于(yu)(yu)(yu)(yu)(yu)(yu)(yu)各反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)2M硫(liu)酸(suan)(suan)0.05ml6、結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)判(pan)定(ding)(ding)(ding)(ding)：可(ke)(ke)(ke)(ke)(ke)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)白(bai)(bai)(bai)(bai)色(se)(se)(se)(se)(se)背景上(shang)(shang)(shang)(shang)(shang)，直(zhi)接用(yong)(yong)(yong)(yong)肉眼觀察(cha)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)：反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)(nei)顏色(se)(se)(se)(se)(se)越(yue)深(shen)(shen)，陽(yang)(yang)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)程度(du)(du)(du)(du)越(yue)強(qiang)，陰(yin)(yin)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)為(wei)(wei)(wei)(wei)(wei)(wei)無色(se)(se)(se)(se)(se)或(huo)(huo)(huo)(huo)極淺(qian)(qian)(qian)，依據(ju)(ju)(ju)所(suo)(suo)呈顏色(se)(se)(se)(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深(shen)(shen)淺(qian)(qian)(qian)，以(yi)(yi)(yi)(yi)“+”、“-”號(hao)表(biao)(biao)(biao)示(shi)(shi)。也(ye)可(ke)(ke)(ke)(ke)(ke)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)OD值：在(zai)(zai)(zai)(zai)ELISA檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)儀(yi)上(shang)(shang)(shang)(shang)(shang)，于(yu)(yu)(yu)(yu)(yu)(yu)(yu)450nm(若(ruo)以(yi)(yi)(yi)(yi)ABTS顯(xian)(xian)色(se)(se)(se)(se)(se)，則(ze)410nm)處，以(yi)(yi)(yi)(yi)空(kong)(kong)(kong)白(bai)(bai)(bai)(bai)對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)調零后(hou)(hou)(hou)(hou)(hou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)OD值，若(ruo)大于(yu)(yu)(yu)(yu)(yu)(yu)(yu)規(gui)定(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)陰(yin)(yin)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao)OD值的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)2.1倍(bei)，即(ji)為(wei)(wei)(wei)(wei)(wei)(wei)陽(yang)(yang)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)。1、用(yong)(yong)(yong)(yong)包(bao)被(bei)(bei)(bei)緩(huan)(huan)沖液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)將(jiang)(jiang)(jiang)(jiang)(jiang)已(yi)(yi)知(zhi)抗(kang)(kang)(kang)(kang)(kang)原(yuan)(yuan)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)至(zhi)(zhi)1-10ug/ml， 每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)0.1ml，4℃過夜(ye)。次(ci)(ci)(ci)(ci)(ci)日(ri)洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)3次(ci)(ci)(ci)(ci)(ci)。2、加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：加(jia)(jia)(jia)(jia)(jia)(jia)(jia)一(yi)(yi)(yi)定(ding)(ding)(ding)(ding)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)待(dai)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(未(wei)(wei)知(zhi)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti))0.05ml于(yu)(yu)(yu)(yu)(yu)(yu)(yu)上(shang)(shang)(shang)(shang)(shang)述已(yi)(yi)包(bao)被(bei)(bei)(bei)之反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong),置(zhi)37℃孵(fu)(fu)(fu)育1小(xiao)(xiao)時(shi),洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)。(同時(shi)做(zuo)(zuo)空(kong)(kong)(kong)白(bai)(bai)(bai)(bai)、陰(yin)(yin)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)及(ji)(ji)(ji)(ji)(ji)陽(yang)(yang)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao))3、加(jia)(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)：于(yu)(yu)(yu)(yu)(yu)(yu)(yu)反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)新(xin)鮮(xian)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)第(di)(di)(di)二(er)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)(抗(kang)(kang)(kang)(kang)(kang)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti))0.05ml，37℃孵(fu)(fu)(fu)育30-60分(fen)(fen)(fen)(fen)鐘(zhong)，洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di),＊后(hou)(hou)(hou)(hou)(hou)一(yi)(yi)(yi)遍用(yong)(yong)(yong)(yong)DDW洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)。4、加(jia)(jia)(jia)(jia)(jia)(jia)(jia)底物(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)顯(xian)(xian)色(se)(se)(se)(se)(se)：于(yu)(yu)(yu)(yu)(yu)(yu)(yu)各反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)臨時(shi)配(pei)制(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)TMB底物(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)0.1ml，37℃ 10～30分(fen)(fen)(fen)(fen)鐘(zhong)。5、終(zhong)止反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)：于(yu)(yu)(yu)(yu)(yu)(yu)(yu)各反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)2M硫(liu)酸(suan)(suan)0.05ml6、結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)判(pan)定(ding)(ding)(ding)(ding)：可(ke)(ke)(ke)(ke)(ke)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)白(bai)(bai)(bai)(bai)色(se)(se)(se)(se)(se)背景上(shang)(shang)(shang)(shang)(shang)，直(zhi)接用(yong)(yong)(yong)(yong)肉眼觀察(cha)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)：反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)(nei)顏色(se)(se)(se)(se)(se)越(yue)深(shen)(shen)，陽(yang)(yang)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)程度(du)(du)(du)(du)越(yue)強(qiang)，陰(yin)(yin)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)為(wei)(wei)(wei)(wei)(wei)(wei)無色(se)(se)(se)(se)(se)或(huo)(huo)(huo)(huo)極淺(qian)(qian)(qian)，依據(ju)(ju)(ju)所(suo)(suo)呈顏色(se)(se)(se)(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深(shen)(shen)淺(qian)(qian)(qian)，以(yi)(yi)(yi)(yi)“+”、“-”號(hao)表(biao)(biao)(biao)示(shi)(shi)。也(ye)可(ke)(ke)(ke)(ke)(ke)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)OD值：在(zai)(zai)(zai)(zai)ELISA檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)儀(yi)上(shang)(shang)(shang)(shang)(shang)，于(yu)(yu)(yu)(yu)(yu)(yu)(yu)450nm(若(ruo)以(yi)(yi)(yi)(yi)ABTS顯(xian)(xian)色(se)(se)(se)(se)(se)，則(ze)410nm)處，以(yi)(yi)(yi)(yi)空(kong)(kong)(kong)白(bai)(bai)(bai)(bai)對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)調零后(hou)(hou)(hou)(hou)(hou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)OD值，若(ruo)大于(yu)(yu)(yu)(yu)(yu)(yu)(yu)規(gui)定(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)陰(yin)(yin)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao)OD值的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)2.1倍(bei)，即(ji)為(wei)(wei)(wei)(wei)(wei)(wei)陽(yang)(yang)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)。 ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)需(xu)自備(bei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)設備(bei)及(ji)(ji)(ji)(ji)(ji)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)    1. 450±10nm濾光片的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)(建(jian)議儀(yi)器(qi)(qi)(qi)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前提前預(yu)(yu)熱)2. 單道或(huo)(huo)(huo)(huo)多(duo)(duo)(duo)道微量(liang)(liang)(liang)(liang)(liang)(liang)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)器(qi)(qi)(qi)及(ji)(ji)(ji)(ji)(ji)吸(xi)(xi)頭(tou)3. 稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)EP管4. 蒸餾(liu)水(shui)或(huo)(huo)(huo)(huo)去(qu)(qu)離(li)子水(shui)5. 吸(xi)(xi)水(shui)紙(zhi)6. 盛放洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)容器(qi)(qi)(qi) 性(xing)(xing)(xing)(xing)(xing)(xing)(xing)能(neng):1．準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)(xian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)回(hui)歸與(yu)預(yu)(yu)期(qi)(qi)(qi)濃(nong)(nong)度(du)(du)(du)(du)相(xiang)關(guan)(guan)系(xi)數R值，大于(yu)(yu)(yu)(yu)(yu)(yu)(yu)等(deng)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)0.9900。2．靈敏(min)度(du)(du)(du)(du)：檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)濃(nong)(nong)度(du)(du)(du)(du)小(xiao)(xiao)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)10 pg/ml。3．特(te)異(yi)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)：不(bu)(bu)與(yu)其(qi)(qi)(qi)(qi)它(ta)可(ke)(ke)(ke)(ke)(ke)溶(rong)(rong)(rong)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)構類似物(wu)(wu)(wu)(wu)(wu)(wu)交叉反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)。4．重(zhong)復(fu)(fu)(fu)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)：板(ban)(ban)(ban)內(nei)(nei)(nei)(nei)(nei)(nei)、板(ban)(ban)(ban)間(jian)(jian)變(bian)異(yi)系(xi)數均(jun)小(xiao)(xiao)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)15%。 ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)優(you)點(dian)(dian)：1、高(gao)(gao)(gao)效(xiao)、靈敏(min)、特(te)異(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)；2、重(zhong)復(fu)(fu)(fu)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)和(he)(he)可(ke)(ke)(ke)(ke)(ke)靠性(xing)(xing)(xing)(xing)(xing)(xing)(xing)高(gao)(gao)(gao)；3、吸(xi)(xi)附(fu)(fu)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)能(neng)好(hao)(hao)(hao)，空(kong)(kong)(kong)白(bai)(bai)(bai)(bai)值低(di)，孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底透(tou)明(ming)度(du)(du)(du)(du)高(gao)(gao)(gao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)固相(xiang)載體(ti)(ti)(ti)(ti)(ti)(ti)；4、適用(yong)(yong)(yong)(yong)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)、血(xue)(xue)(xue)漿(jiang)(jiang)、組(zu)織(zhi)(zhi)(zhi)勻(yun)(yun)漿(jiang)(jiang)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、細(xi)胞(bao)(bao)(bao)培(pei)養上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、尿(niao)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)等(deng)等(deng)多(duo)(duo)(duo)種標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類型；5、可(ke)(ke)(ke)(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)指(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)齊全：細(xi)胞(bao)(bao)(bao)因(yin)(yin)子檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)、心(xin)(xin)(xin)(xin)肌梗塞檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)、內(nei)(nei)(nei)(nei)(nei)(nei)分(fen)(fen)(fen)(fen)泌檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)、肝纖維化檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)、自身免(mian)(mian)(mian)疫(yi)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)、腫瘤(liu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)志物(wu)(wu)(wu)(wu)(wu)(wu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)、傳染病檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)、特(te)種蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)、優(you)生(sheng)(sheng)優(you)育檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)等(deng)等(deng)；6、價格(ge)(ge)優(you)惠(hui)，質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)保(bao)(bao)證(zheng)，限(xian)(xian)度(du)(du)(du)(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)節省實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)經費。 實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)步(bu)(bu)(bu)驟(zou)1. 使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前將(jiang)(jiang)(jiang)(jiang)(jiang)所(suo)(suo)有(you)(you)(you)(you)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)和(he)(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)緩(huan)(huan)慢均(jun)衡至(zhi)(zhi)室(shi)(shi)溫(wen)(wen)(wen)(18-25oC)，試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)不(bu)(bu)能(neng)直(zhi)接在(zai)(zai)(zai)(zai)37oC溶(rong)(rong)(rong)解(jie)(jie)。2. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(凍(dong)干(gan)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin))：每(mei)瓶(ping)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)1mL，蓋好(hao)(hao)(hao)后(hou)(hou)(hou)(hou)(hou)室(shi)(shi)溫(wen)(wen)(wen)靜置(zhi)大約10分(fen)(fen)(fen)(fen)鐘(zhong)，同時(shi)反(fan)(fan)(fan)(fan)(fan)(fan)復(fu)(fu)(fu)顛倒/搓動以(yi)(yi)(yi)(yi)助溶(rong)(rong)(rong)解(jie)(jie)，其(qi)(qi)(qi)(qi)濃(nong)(nong)度(du)(du)(du)(du)為(wei)(wei)(wei)(wei)(wei)(wei)2000pg/mL。準(zhun)(zhun)(zhun)(zhun)(zhun)備(bei)7個(ge)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)EP管，每(mei)個(ge)EP管中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)150μL的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，如(ru)(ru)(ru)圖(tu)(tu)(tu)所(suo)(suo)示(shi)(shi)依次(ci)(ci)(ci)(ci)(ci)倍(bei)比(bi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)成(cheng)(cheng)不(bu)(bu)同的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)(du)， 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(0pg/mL)直(zhi)接作(zuo)為(wei)(wei)(wei)(wei)(wei)(wei)空(kong)(kong)(kong)白(bai)(bai)(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。為(wei)(wei)(wei)(wei)(wei)(wei)保(bao)(bao)證(zheng)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)有(you)(you)(you)(you)效(xiao)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)，每(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)請(qing)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)新(xin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 3. 濃(nong)(nong)洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)：用(yong)(yong)(yong)(yong)580mL蒸餾(liu)水(shui)或(huo)(huo)(huo)(huo)去(qu)(qu)離(li)子水(shui)將(jiang)(jiang)(jiang)(jiang)(jiang)20mL濃(nong)(nong)洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)至(zhi)(zhi)600mL，進行(xing)30倍(bei)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)。 注(zhu)意事(shi)項(xiang)(xiang)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)儲存(cun)及(ji)(ji)(ji)(ji)(ji)有(you)(you)(you)(you)效(xiao)期(qi)(qi)(qi)1. 未(wei)(wei)開封的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)：所(suo)(suo)有(you)(you)(you)(you)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)均(jun)按(an)(an)(an)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)瓶(ping)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽上(shang)(shang)(shang)(shang)(shang)所(suo)(suo)示(shi)(shi)保(bao)(bao)存(cun)。請(qing)注(zhu)意，收到(dao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)后(hou)(hou)(hou)(hou)(hou)請(qing)盡快(kuai)將(jiang)(jiang)(jiang)(jiang)(jiang)TMB 洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)保(bao)(bao)存(cun)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)4℃，其(qi)(qi)(qi)(qi)他試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)保(bao)(bao)存(cun)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)-20℃。2. 使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)后(hou)(hou)(hou)(hou)(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)：剩余試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)仍需(xu)按(an)(an)(an)照(zhao)(zhao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)瓶(ping)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽所(suo)(suo)示(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)溫(wen)(wen)(wen)度(du)(du)(du)(du)保(bao)(bao)存(cun)，開封后(hou)(hou)(hou)(hou)(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)要(yao)(yao)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)干(gan)燥劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)后(hou)(hou)(hou)(hou)(hou)密(mi)封保(bao)(bao)存(cun)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)-20℃，避(bi)免(mian)(mian)(mian)潮(chao)濕。 須知(zhi)：試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)內(nei)(nei)(nei)(nei)(nei)(nei)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)條可(ke)(ke)(ke)(ke)(ke)拆(chai)卸，按(an)(an)(an)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)需(xu)求(qiu)可(ke)(ke)(ke)(ke)(ke)分(fen)(fen)(fen)(fen)多(duo)(duo)(duo)次(ci)(ci)(ci)(ci)(ci)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)；使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)后(hou)(hou)(hou)(hou)(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)剩余試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)建(jian)議在(zai)(zai)(zai)(zai)首次(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)后(hou)(hou)(hou)(hou)(hou)1個(ge)月內(nei)(nei)(nei)(nei)(nei)(nei)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)完畢。產品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)過期(qi)(qi)(qi)時(shi)間(jian)(jian)以(yi)(yi)(yi)(yi)盒(he)(he)(he)(he)子上(shang)(shang)(shang)(shang)(shang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽為(wei)(wei)(wei)(wei)(wei)(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)，保(bao)(bao)質(zhi)(zhi)(zhi)(zhi)期(qi)(qi)(qi)內(nei)(nei)(nei)(nei)(nei)(nei)所(suo)(suo)有(you)(you)(you)(you)組(zu)分(fen)(fen)(fen)(fen)都(dou)確(que)保(bao)(bao)是穩定(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。 ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)報1、ELISA標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)；2、詳細(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)步(bu)(bu)(bu)驟(zou)；3、完整的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)報告(gao)（試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)耗材(cai)，實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)方(fang)法(fa)，實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)及(ji)(ji)(ji)(ji)(ji)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)說明(ming)）； [樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)處理及(ji)(ji)(ji)(ji)(ji)要(yao)(yao)求(qiu)]1.  血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)：將(jiang)(jiang)(jiang)(jiang)(jiang)收集于(yu)(yu)(yu)(yu)(yu)(yu)(yu)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)分(fen)(fen)(fen)(fen)離(li)管的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)全血(xue)(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)在(zai)(zai)(zai)(zai)室(shi)(shi)溫(wen)(wen)(wen)放置(zhi)2小(xiao)(xiao)時(shi)或(huo)(huo)(huo)(huo)4℃過夜(ye)，然后(hou)(hou)(hou)(hou)(hou)1000×g離(li)心(xin)(xin)(xin)(xin)20 分(fen)(fen)(fen)(fen)鐘(zhong)，取(qu)(qu)上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)即(ji)可(ke)(ke)(ke)(ke)(ke)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)(jiang)上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)置(zhi)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)避(bi)免(mian)(mian)(mian)反(fan)(fan)(fan)(fan)(fan)(fan)復(fu)(fu)(fu)凍(dong)融(rong)。2.  血(xue)(xue)(xue)漿(jiang)(jiang)：用(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)肝素(su)(su)(su)作(zuo)為(wei)(wei)(wei)(wei)(wei)(wei)抗(kang)(kang)(kang)(kang)(kang)凝劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)采集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)，并(bing)(bing)(bing)將(jiang)(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)在(zai)(zai)(zai)(zai)采集后(hou)(hou)(hou)(hou)(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)30分(fen)(fen)(fen)(fen)鐘(zhong)內(nei)(nei)(nei)(nei)(nei)(nei)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)2-8℃ 1000×g離(li)心(xin)(xin)(xin)(xin)15分(fen)(fen)(fen)(fen)鐘(zhong)，取(qu)(qu)上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)即(ji)可(ke)(ke)(ke)(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)(jiang)上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)置(zhi)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)避(bi)免(mian)(mian)(mian)反(fan)(fan)(fan)(fan)(fan)(fan)復(fu)(fu)(fu)凍(dong)融(rong)。3.  組(zu)織(zhi)(zhi)(zhi)勻(yun)(yun)漿(jiang)(jiang)：用(yong)(yong)(yong)(yong)預(yu)(yu)冷的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)PBS (0.01M, pH=7.4)沖洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)組(zu)織(zhi)(zhi)(zhi)，去(qu)(qu)除(chu)殘留(liu)血(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)（勻(yun)(yun)漿(jiang)(jiang)中(zhong)(zhong)(zhong)(zhong)裂解(jie)(jie)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)紅細(xi)胞(bao)(bao)(bao)會(hui)(hui)(hui)影(ying)響測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)(liang)(liang)(liang)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)），稱重(zhong)后(hou)(hou)(hou)(hou)(hou)將(jiang)(jiang)(jiang)(jiang)(jiang)組(zu)織(zhi)(zhi)(zhi)剪碎。將(jiang)(jiang)(jiang)(jiang)(jiang)剪碎的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)織(zhi)(zhi)(zhi)與(yu)對(dui)(dui)(dui)(dui)(dui)應(ying)(ying)(ying)(ying)(ying)(ying)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)PBS（一(yi)(yi)(yi)般按(an)(an)(an)1:9的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)重(zhong)量(liang)(liang)(liang)(liang)(liang)(liang)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)比(bi)，比(bi)如(ru)(ru)(ru)1g的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)織(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)對(dui)(dui)(dui)(dui)(dui)應(ying)(ying)(ying)(ying)(ying)(ying)9mL的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)PBS，具體(ti)(ti)(ti)(ti)(ti)(ti)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)可(ke)(ke)(ke)(ke)(ke)根據(ju)(ju)(ju)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)需(xu)要(yao)(yao)適當(dang)調整，并(bing)(bing)(bing)做(zuo)(zuo)好(hao)(hao)(hao)記錄。推薦(jian)在(zai)(zai)(zai)(zai)PBS中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)酶(mei)(mei)(mei)(mei)(mei)抑制(zhi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)）加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)玻(bo)璃勻(yun)(yun)漿(jiang)(jiang)器(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)，于(yu)(yu)(yu)(yu)(yu)(yu)(yu)冰上(shang)(shang)(shang)(shang)(shang)充分(fen)(fen)(fen)(fen)研(yan)磨。為(wei)(wei)(wei)(wei)(wei)(wei)了(le)進一(yi)(yi)(yi)步(bu)(bu)(bu)裂解(jie)(jie)組(zu)織(zhi)(zhi)(zhi)細(xi)胞(bao)(bao)(bao)，可(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)對(dui)(dui)(dui)(dui)(dui)勻(yun)(yun)漿(jiang)(jiang)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)進行(xing)超(chao)聲(sheng)破碎，或(huo)(huo)(huo)(huo)反(fan)(fan)(fan)(fan)(fan)(fan)復(fu)(fu)(fu)凍(dong)融(rong)。＊后(hou)(hou)(hou)(hou)(hou)將(jiang)(jiang)(jiang)(jiang)(jiang)勻(yun)(yun)漿(jiang)(jiang)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)5000×g離(li)心(xin)(xin)(xin)(xin)5~10分(fen)(fen)(fen)(fen)鐘(zhong)，取(qu)(qu)上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。4.  細(xi)胞(bao)(bao)(bao)培(pei)養物(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)或(huo)(huo)(huo)(huo)其(qi)(qi)(qi)(qi)它(ta)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)：請(qing)1000×g離(li)心(xin)(xin)(xin)(xin)20分(fen)(fen)(fen)(fen)鐘(zhong)，取(qu)(qu)上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)即(ji)可(ke)(ke)(ke)(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)(jiang)上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)置(zhi)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)，但應(ying)(ying)(ying)(ying)(ying)(ying)避(bi)免(mian)(mian)(mian)反(fan)(fan)(fan)(fan)(fan)(fan)復(fu)(fu)(fu)凍(dong)融(rong)。注(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)溶(rong)(rong)(rong)血(xue)(xue)(xue)會(hui)(hui)(hui)影(ying)響＊后(hou)(hou)(hou)(hou)(hou)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)，因(yin)(yin)此(ci)(ci)(ci)溶(rong)(rong)(rong)血(xue)(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)不(bu)(bu)宜進行(xing)此(ci)(ci)(ci)項(xiang)(xiang)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。 [需(xu)要(yao)(yao)而未(wei)(wei)提供(gong)(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)和(he)(he)器(qi)(qi)(qi)材(cai)]1.酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)（450nm）2.高(gao)(gao)(gao)精度(du)(du)(du)(du)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)器(qi)(qi)(qi)及(ji)(ji)(ji)(ji)(ji)槍頭(tou)：0.5-10uL、2-20uL、20-200uL、200-1000uL3.37℃恒溫(wen)(wen)(wen)箱4.蒸餾(liu)水(shui)或(huo)(huo)(huo)(huo)去(qu)(qu)離(li)子水(shui) ELISA檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)承(cheng)諾公(gong)司(si)(si)長期(qi)(qi)(qi)與(yu)各大高(gao)(gao)(gao)校(xiao)、醫院及(ji)(ji)(ji)(ji)(ji)權威科(ke)(ke)研(yan)單位保(bao)(bao)持著(zhu)良(liang)好(hao)(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)合(he)(he)(he)(he)作(zuo)關(guan)(guan)系(xi)，公(gong)司(si)(si)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)及(ji)(ji)(ji)(ji)(ji)服(fu)(fu)(fu)務(wu)(wu)(wu)態度(du)(du)(du)(du)得(de)(de)到(dao)了(le)他們的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)致認(ren)可(ke)(ke)(ke)(ke)(ke)。篤(du)瑪生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)著(zhu)客戶(hu)至(zhi)(zhi)上(shang)(shang)(shang)(shang)(shang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)(yuan)則(ze)為(wei)(wei)(wei)(wei)(wei)(wei)客戶(hu)提供(gong)(gong)優(you)質(zhi)(zhi)(zhi)(zhi)產品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)，公(gong)司(si)(si)承(cheng)諾產品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)有(you)(you)(you)(you)任(ren)(ren)(ren)何(he)質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)問(wen)題(ti)免(mian)(mian)(mian)費包(bao)退(tui)換(huan)（非人(ren)為(wei)(wei)(wei)(wei)(wei)(wei)因(yin)(yin)素(su)(su)(su)造(zao)成(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)）。公(gong)司(si)(si)提供(gong)(gong)免(mian)(mian)(mian)費代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)服(fu)(fu)(fu)務(wu)(wu)(wu)，并(bing)(bing)(bing)且承(cheng)諾收到(dao)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)七個(ge)工(gong)(gong)作(zuo)日(ri)出結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)，確(que)保(bao)(bao)數據(ju)(ju)(ju)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)(xing)(xing)(xing)(xing)(xing) 小(xiao)(xiao)鼠纖連(lian)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)1(FN-1)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)原(yuan)(yuan)理：本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)是固相(xiang)夾(jia)心(xin)(xin)(xin)(xin)法(fa)酶(mei)(mei)(mei)(mei)(mei)聯免(mian)(mian)(mian)疫(yi)吸(xi)(xi)附(fu)(fu)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)（ELISA）.已(yi)(yi)知(zhi)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)濃(nong)(nong)度(du)(du)(du)(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、未(wei)(wei)知(zhi)濃(nong)(nong)度(du)(du)(du)(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)內(nei)(nei)(nei)(nei)(nei)(nei)進行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。先將(jiang)(jiang)(jiang)(jiang)(jiang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)和(he)(he)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)同時(shi)溫(wen)(wen)(wen)育。洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)后(hou)(hou)(hou)(hou)(hou)，加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)親和(he)(he)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記過的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)HRP。再經過溫(wen)(wen)(wen)育和(he)(he)洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)，去(qu)(qu)除(chu)未(wei)(wei)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)合(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)合(he)(he)(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)，然后(hou)(hou)(hou)(hou)(hou)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)底物(wu)(wu)(wu)(wu)(wu)(wu)A、B，和(he)(he)酶(mei)(mei)(mei)(mei)(mei)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)合(he)(he)(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)同時(shi)作(zuo)用(yong)(yong)(yong)(yong)。產生(sheng)(sheng)顏色(se)(se)(se)(se)(se)。顏色(se)(se)(se)(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深(shen)(shen)淺(qian)(qian)(qian)和(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)(zhong)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)呈比(bi)例關(guan)(guan)系(xi)。完整的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)包(bao)含(han)以(yi)(yi)(yi)(yi)下各組(zu)分(fen)(fen)(fen)(fen)：（1）已(yi)(yi)包(bao)被(bei)(bei)(bei)抗(kang)(kang)(kang)(kang)(kang)原(yuan)(yuan)或(huo)(huo)(huo)(huo)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)固相(xiang)載體(ti)(ti)(ti)(ti)(ti)(ti)（免(mian)(mian)(mian)疫(yi)吸(xi)(xi)附(fu)(fu)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)）；（2）酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)抗(kang)(kang)(kang)(kang)(kang)原(yuan)(yuan)或(huo)(huo)(huo)(huo)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)（結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)合(he)(he)(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)）；（3）酶(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底物(wu)(wu)(wu)(wu)(wu)(wu)；（4）陰(yin)(yin)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)和(he)(he)陽(yang)(yang)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)（定(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)），參考(kao)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)和(he)(he)控制(zhi)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)（定(ding)(ding)(ding)(ding)量(liang)(liang)(liang)(liang)(liang)(liang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)）；（5）結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)合(he)(he)(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)及(ji)(ji)(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)；（6）洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，在(zai)(zai)(zai)(zai)板(ban)(ban)(ban)式(shi)(shi)ELISA中(zhong)(zhong)(zhong)(zhong)，常用(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)為(wei)(wei)(wei)(wei)(wei)(wei)含(han)0.05%吐溫(wen)(wen)(wen)20磷酸(suan)(suan)緩(huan)(huan)沖鹽水(shui)；（7）酶(mei)(mei)(mei)(mei)(mei)反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，常用(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)HRP反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)為(wei)(wei)(wei)(wei)(wei)(wei)硫(liu)酸(suan)(suan)，其(qi)(qi)(qi)(qi)濃(nong)(nong)度(du)(du)(du)(du)按(an)(an)(an)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)量(liang)(liang)(liang)(liang)(liang)(liang)及(ji)(ji)(ji)(ji)(ji)比(bi)色(se)(se)(se)(se)(se)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)*終(zhong)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)而異(yi)，在(zai)(zai)(zai)(zai)板(ban)(ban)(ban)式(shi)(shi)ELISA中(zhong)(zhong)(zhong)(zhong)一(yi)(yi)(yi)般采用(yong)(yong)(yong)(yong)2mol/L。 ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)數據(ju)(ju)(ju)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計算(suan)處理方(fang)法(fa)1、擬和(he)(he)曲(qu)線(xian)(xian)(xian)：輸入(ru)(ru)第(di)(di)(di)一(yi)(yi)(yi)行(xing)： 濃(nong)(nong)度(du)(du)(du)(du)值， 如(ru)(ru)(ru)0 10 50 100 400輸入(ru)(ru)第(di)(di)(di)二(er)行(xing)：該濃(nong)(nong)度(du)(du)(du)(du)下的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)調整后(hou)(hou)(hou)(hou)(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)od值，如(ru)(ru)(ru)0 0.586 1.397 1.997 3.42選(xuan)(xuan)擇(ze)這(zhe)些輸入(ru)(ru)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數據(ju)(ju)(ju)，用(yong)(yong)(yong)(yong)插(cha)入(ru)(ru)里的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)圖(tu)(tu)(tu)表(biao)(biao)(biao)按(an)(an)(an)鈕，進入(ru)(ru)圖(tu)(tu)(tu)表(biao)(biao)(biao)向導(dao)，在(zai)(zai)(zai)(zai)“標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)類型”中(zhong)(zhong)(zhong)(zhong)選(xuan)(xuan)擇(ze)“xy散(san)點(dian)(dian)圖(tu)(tu)(tu)”;在(zai)(zai)(zai)(zai)“子圖(tu)(tu)(tu)表(biao)(biao)(biao)類型”中(zhong)(zhong)(zhong)(zhong)選(xuan)(xuan)擇(ze)“折線(xian)(xian)(xian)散(san)點(dian)(dian)圖(tu)(tu)(tu)”，按(an)(an)(an)“下一(yi)(yi)(yi)步(bu)(bu)(bu)”;選(xuan)(xuan)擇(ze)“系(xi)列產生(sheng)(sheng)在(zai)(zai)(zai)(zai)行(xing)”，按(an)(an)(an)“下一(yi)(yi)(yi)步(bu)(bu)(bu)”;數據(ju)(ju)(ju)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)志，可(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)填寫：如(ru)(ru)(ru)數據(ju)(ju)(ju)y軸(zhou)，OD值;數據(ju)(ju)(ju)x軸(zhou)，濃(nong)(nong)度(du)(du)(du)(du);按(an)(an)(an)下一(yi)(yi)(yi)步(bu)(bu)(bu)，點(dian)(dian)擊(ji)(ji)完成(cheng)(cheng)。可(ke)(ke)(ke)(ke)(ke)得(de)(de)曲(qu)線(xian)(xian)(xian)圖(tu)(tu)(tu)。單擊(ji)(ji)曲(qu)線(xian)(xian)(xian)，按(an)(an)(an)右鍵(jian)，選(xuan)(xuan)擇(ze)“添加(jia)(jia)(jia)(jia)(jia)(jia)(jia)趨勢(shi)線(xian)(xian)(xian)”，在(zai)(zai)(zai)(zai)類型中(zhong)(zhong)(zhong)(zhong)，選(xuan)(xuan)擇(ze)多(duo)(duo)(duo)項(xiang)(xiang)式(shi)(shi);在(zai)(zai)(zai)(zai)選(xuan)(xuan)項(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)，選(xuan)(xuan)擇(ze)顯(xian)(xian)示(shi)(shi)公(gong)式(shi)(shi)，選(xuan)(xuan)擇(ze)顯(xian)(xian)示(shi)(shi)R平(ping)方(fang)值。得(de)(de)到(dao)公(gong)式(shi)(shi)和(he)(he)R平(ping)方(fang)值。也(ye)可(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)用(yong)(yong)(yong)(yong)上(shang)(shang)(shang)(shang)(shang)面說的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)法(fa)： 雙擊(ji)(ji)圖(tu)(tu)(tu)表(biao)(biao)(biao)，把(ba)它(ta)輸入(ru)(ru)到(dao)圖(tu)(tu)(tu)表(biao)(biao)(biao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數據(ju)(ju)(ju)中(zhong)(zhong)(zhong)(zhong)，就(jiu)可(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)擬和(he)(he)曲(qu)線(xian)(xian)(xian)。 2、計算(suan)濃(nong)(nong)度(du)(du)(du)(du)：第(di)(di)(di)一(yi)(yi)(yi)次(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)為(wei)(wei)(wei)(wei)(wei)(wei)：y = -4E-05x2 + 0.026xR2 = 0.9745為(wei)(wei)(wei)(wei)(wei)(wei)例，已(yi)(yi)知(zhi)OD值，計算(suan)濃(nong)(nong)度(du)(du)(du)(du)。由(you)(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)y = -4E-05x2 + 0.026x，可(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)得(de)(de)到(dao)：4E-05x2 -0.026x +y=0ax2 +bx +y=0特(te)別說明(ming):#.一(yi)(yi)(yi)周內(nei)(nei)(nei)(nei)(nei)(nei)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)可(ke)(ke)(ke)(ke)(ke)存(cun)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)4℃，需(xu)長時(shi)間(jian)(jian)存(cun)放或(huo)(huo)(huo)(huo)多(duo)(duo)(duo)次(ci)(ci)(ci)(ci)(ci)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)建(jian)議存(cun)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)-20℃.化學(xue)發(fa)光免(mian)(mian)(mian)疫(yi)分(fen)(fen)(fen)(fen)析(xi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)1.在(zai)(zai)(zai)(zai)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)各100μL，37°C孵(fu)(fu)(fu)育90分(fen)(fen)(fen)(fen)鐘(zhong)2.倒去(qu)(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)，拍(pai)干(gan)，加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)100μL生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)化抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，37°C孵(fu)(fu)(fu)育 60分(fen)(fen)(fen)(fen)鐘(zhong)3.洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)3次(ci)(ci)(ci)(ci)(ci)4.加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru) 100μL酶(mei)(mei)(mei)(mei)(mei)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)合(he)(he)(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，37°C孵(fu)(fu)(fu)育 30分(fen)(fen)(fen)(fen)鐘(zhong)5.洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)5次(ci)(ci)(ci)(ci)(ci)6.加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)100μL 發(fa)光底物(wu)(wu)(wu)(wu)(wu)(wu)混(hun)合(he)(he)(he)(he)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，37°C孵(fu)(fu)(fu)育5分(fen)(fen)(fen)(fen)鐘(zhong)左右7.立即(ji)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)化學(xue)發(fa)光值8.結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)計算(suan) ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)優(you)勢(shi)：1、抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)----高(gao)(gao)(gao)效(xiao)、靈敏(min)、特(te)異(yi)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)好(hao)(hao)(hao)2、規(gui)范包(bao)被(bei)(bei)(bei)操(cao)(cao)作(zuo)----吸(xi)(xi)附(fu)(fu)均(jun)勻(yun)(yun)、吸(xi)(xi)附(fu)(fu)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)好(hao)(hao)(hao)、空(kong)(kong)(kong)白(bai)(bai)(bai)(bai)值低(di)、孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底透(tou)明(ming)度(du)(du)(du)(du)高(gao)(gao)(gao)3、優(you)化方(fang)案----重(zhong)復(fu)(fu)(fu)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)高(gao)(gao)(gao)，可(ke)(ke)(ke)(ke)(ke)靠性(xing)(xing)(xing)(xing)(xing)(xing)(xing)強(qiang)4、高(gao)(gao)(gao)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)技(ji)術(shu)服(fu)(fu)(fu)務(wu)(wu)(wu)要(yao)(yao)求(qiu)：靈敏(min)度(du)(du)(du)(du)高(gao)(gao)(gao)，特(te)異(yi)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)強(qiang) 5、適用(yong)(yong)(yong)(yong)范圍廣：血(xue)(xue)(xue)漿(jiang)(jiang)、血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)、組(zu)織(zhi)(zhi)(zhi)勻(yun)(yun)漿(jiang)(jiang)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、細(xi)胞(bao)(bao)(bao)培(pei)養上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、尿(niao)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)等(deng)多(duo)(duo)(duo)種類型的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben) ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)操(cao)(cao)作(zuo)步(bu)(bu)(bu)驟(zou)：1.使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前，將(jiang)(jiang)(jiang)(jiang)(jiang)所(suo)(suo)有(you)(you)(you)(you)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)充分(fen)(fen)(fen)(fen)混(hun)勻(yun)(yun)。不(bu)(bu)要(yao)(yao)使(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)產生(sheng)(sheng)大量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)泡(pao)沫(mo)，以(yi)(yi)(yi)(yi)免(mian)(mian)(mian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)時(shi)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)大量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)氣泡(pao)，產生(sheng)(sheng)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)上(shang)(shang)(shang)(shang)(shang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)誤差。2.根據(ju)(ju)(ju)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)數量(liang)(liang)(liang)(liang)(liang)(liang)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)(shang)(shang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數量(liang)(liang)(liang)(liang)(liang)(liang)決定(ding)(ding)(ding)(ding)所(suo)(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)板(ban)(ban)(ban)條數。每(mei)個(ge)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)和(he)(he)空(kong)(kong)(kong)白(bai)(bai)(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)建(jian)議做(zuo)(zuo)復(fu)(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。每(mei)個(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)根據(ju)(ju)(ju)自己的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數量(liang)(liang)(liang)(liang)(liang)(liang)來定(ding)(ding)(ding)(ding)，能(neng)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)復(fu)(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)盡量(liang)(liang)(liang)(liang)(liang)(liang)做(zuo)(zuo)復(fu)(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)1：1稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)50ul于(yu)(yu)(yu)(yu)(yu)(yu)(yu)反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)(nei)。3.加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)好(hao)(hao)(hao)后(hou)(hou)(hou)(hou)(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)50ul于(yu)(yu)(yu)(yu)(yu)(yu)(yu)反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)50ul于(yu)(yu)(yu)(yu)(yu)(yu)(yu)反(fan)(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)(nei)。立即(ji)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)50ul的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)*標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)。蓋上(shang)(shang)(shang)(shang)(shang)膜板(ban)(ban)(ban)，輕(qing)(qing)輕(qing)(qing)振蕩(dang)(dang)混(hun)勻(yun)(yun)，37℃溫(wen)(wen)(wen)育1小(xiao)(xiao)時(shi)。4.甩(shuai)(shuai)去(qu)(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)，每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，振蕩(dang)(dang)30秒(miao)，甩(shuai)(shuai)去(qu)(qu)洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，用(yong)(yong)(yong)(yong)吸(xi)(xi)水(shui)紙(zhi)拍(pai)干(gan)。重(zhong)復(fu)(fu)(fu)此(ci)(ci)(ci)操(cao)(cao)作(zuo)3次(ci)(ci)(ci)(ci)(ci)。如(ru)(ru)(ru)果(guo)(guo)(guo)用(yong)(yong)(yong)(yong)洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)機洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)，洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)次(ci)(ci)(ci)(ci)(ci)數增加(jia)(jia)(jia)(jia)(jia)(jia)(jia)一(yi)(yi)(yi)次(ci)(ci)(ci)(ci)(ci)。5.每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)80ul的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)親和(he)(he)鏈酶(mei)(mei)(mei)(mei)(mei)素(su)(su)(su)-HRP，輕(qing)(qing)輕(qing)(qing)振蕩(dang)(dang)混(hun)勻(yun)(yun)，37℃溫(wen)(wen)(wen)育30分(fen)(fen)(fen)(fen)鐘(zhong)。6.甩(shuai)(shuai)去(qu)(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)，每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，振蕩(dang)(dang)30秒(miao)，甩(shuai)(shuai)去(qu)(qu)洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，用(yong)(yong)(yong)(yong)吸(xi)(xi)水(shui)紙(zhi)拍(pai)干(gan)。重(zhong)復(fu)(fu)(fu)此(ci)(ci)(ci)操(cao)(cao)作(zuo)3次(ci)(ci)(ci)(ci)(ci)。如(ru)(ru)(ru)果(guo)(guo)(guo)用(yong)(yong)(yong)(yong)洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)機洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)，洗(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)次(ci)(ci)(ci)(ci)(ci)數增加(jia)(jia)(jia)(jia)(jia)(jia)(jia)一(yi)(yi)(yi)次(ci)(ci)(ci)(ci)(ci)。7.每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)底物(wu)(wu)(wu)(wu)(wu)(wu)A、B各50ul，輕(qing)(qing)輕(qing)(qing)振蕩(dang)(dang)混(hun)勻(yun)(yun)，37℃溫(wen)(wen)(wen)育10分(fen)(fen)(fen)(fen)鐘(zhong)。避(bi)免(mian)(mian)(mian)光照(zhao)(zhao)。8.取(qu)(qu)出酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)，迅(xun)速加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)50ul終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)(hou)應(ying)(ying)(ying)(ying)(ying)(ying)立即(ji)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)。9.在(zai)(zai)(zai)(zai)450nm波長處測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值。 服(fu)(fu)(fu)務(wu)(wu)(wu)承(cheng)諾：一(yi)(yi)(yi)、產品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)質(zhi)(zhi)(zhi)(zhi)保(bao)(bao)障（打造(zao)高(gao)(gao)(gao)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)質(zhi)(zhi)(zhi)(zhi)產品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)，若(ruo)產品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)存(cun)在(zai)(zai)(zai)(zai)任(ren)(ren)(ren)何(he)問(wen)題(ti)，公(gong)司(si)(si)一(yi)(yi)(yi)律包(bao)退(tui)包(bao)換(huan)，質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)有(you)(you)(you)(you)保(bao)(bao)障，解(jie)(jie)除(chu)了(le)客戶(hu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)后(hou)(hou)(hou)(hou)(hou)顧之憂(you)）二(er)、提供(gong)(gong)免(mian)(mian)(mian)費代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)服(fu)(fu)(fu)務(wu)(wu)(wu)（我司(si)(si)擁有(you)(you)(you)(you)專(zhuan)業(ye)酶(mei)(mei)(mei)(mei)(mei)免(mian)(mian)(mian)技(ji)術(shu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)工(gong)(gong)作(zuo)人(ren)員(yuan)為(wei)(wei)(wei)(wei)(wei)(wei)客戶(hu)提供(gong)(gong)來樣(yang)(yang)(yang)(yang)(yang)(yang)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)服(fu)(fu)(fu)務(wu)(wu)(wu),限(xian)(xian)度(du)(du)(du)(du)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)(you)(you)效(xiao)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)）三、提供(gong)(gong)全程技(ji)術(shu)指(zhi)導(dao)（專(zhuan)業(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)科(ke)(ke)順技(ji)術(shu)人(ren)員(yuan)進行(xing)指(zhi)導(dao)）四、全天在(zai)(zai)(zai)(zai)線(xian)(xian)(xian)服(fu)(fu)(fu)務(wu)(wu)(wu)（如(ru)(ru)(ru)果(guo)(guo)(guo)您在(zai)(zai)(zai)(zai)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)ELISA試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)產品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)有(you)(you)(you)(you)任(ren)(ren)(ren)何(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)疑(yi)問(wen)，或(huo)(huo)(huo)(huo)者(zhe)(zhe)對(dui)(dui)(dui)(dui)(dui)我們有(you)(you)(you)(you)任(ren)(ren)(ren)何(he)意見建(jian)議，您都(dou)可(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)通(tong)過來電(dian)或(huo)(huo)(huo)(huo)咨詢。） [說明(ming)]1.由(you)(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)現有(you)(you)(you)(you)條件及(ji)(ji)(ji)(ji)(ji)科(ke)(ke)學(xue)技(ji)術(shu)水(shui)平(ping)尚不(bu)(bu)能(neng)對(dui)(dui)(dui)(dui)(dui)所(suo)(suo)有(you)(you)(you)(you)供(gong)(gong)貨商提供(gong)(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)所(suo)(suo)有(you)(you)(you)(you)原(yuan)(yuan)料進行(xing)全面的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)鑒定(ding)(ding)(ding)(ding)與(yu)分(fen)(fen)(fen)(fen)析(xi)，本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)產品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)可(ke)(ke)(ke)(ke)(ke)能(neng)存(cun)在(zai)(zai)(zai)(zai)一(yi)(yi)(yi)定(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)技(ji)術(shu)風(feng)險。2.＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)與(yu)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)(you)(you)效(xiao)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)、實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)者(zhe)(zhe)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)相(xiang)關(guan)(guan)操(cao)(cao)作(zuo)以(yi)(yi)(yi)(yi)及(ji)(ji)(ji)(ji)(ji)當(dang)時(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)環境密(mi)切相(xiang)關(guan)(guan)，請(qing)務(wu)(wu)(wu)必(bi)準(zhun)(zhun)(zhun)(zhun)(zhun)備(bei)充足的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)備(bei)份(fen)。3.不(bu)(bu)同批次(ci)(ci)(ci)(ci)(ci)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同一(yi)(yi)(yi)產品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)可(ke)(ke)(ke)(ke)(ke)能(neng)會(hui)(hui)(hui)有(you)(you)(you)(you)少許差別，如(ru)(ru)(ru)：檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian)(xian)、靈敏(min)度(du)(du)(du)(du)以(yi)(yi)(yi)(yi)及(ji)(ji)(ji)(ji)(ji)顯(xian)(xian)色(se)(se)(se)(se)(se)時(shi)間(jian)(jian)等(deng)，請(qing)依據(ju)(ju)(ju)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)內(nei)(nei)(nei)(nei)(nei)(nei)說明(ming)書(shu)進行(xing)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)操(cao)(cao)作(zuo)，網站(zhan)電(dian)子版說明(ming)書(shu)僅作(zuo)參考(kao)。4.只(zhi)有(you)(you)(you)(you)全部使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)配(pei)套試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)才能(neng)保(bao)(bao)證(zheng)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)效(xiao)果(guo)(guo)(guo)，不(bu)(bu)能(neng)混(hun)用(yong)(yong)(yong)(yong)其(qi)(qi)(qi)(qi)他制(zhi)造(zao)商的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)。只(zhi)有(you)(you)(you)(you)嚴格(ge)(ge)遵守(shou)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)說明(ming)才會(hui)(hui)(hui)得(de)(de)到(dao)＊佳(jia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)。5.本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)公(gong)司(si)(si)只(zhi)對(dui)(dui)(dui)(dui)(dui)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)身負責，不(bu)(bu)對(dui)(dui)(dui)(dui)(dui)因(yin)(yin)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)該試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)所(suo)(suo)造(zao)成(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)消耗負責，請(qing)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)者(zhe)(zhe)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前充分(fen)(fen)(fen)(fen)考(kao)慮到(dao)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)可(ke)(ke)(ke)(ke)(ke)能(neng)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)量(liang)(liang)(liang)(liang)(liang)(liang)，預(yu)(yu)留(liu)充足的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)。6.使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)化學(xue)裂解(jie)(jie)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)制(zhi)備(bei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)織(zhi)(zhi)(zhi)勻(yun)(yun)漿(jiang)(jiang)或(huo)(huo)(huo)(huo)細(xi)胞(bao)(bao)(bao)提取(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)可(ke)(ke)(ke)(ke)(ke)能(neng)會(hui)(hui)(hui)由(you)(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)某(mou)些化學(xue)物(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)引(yin)入(ru)(ru)導(dao)致ELISA實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)偏差。7.若(ruo)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)為(wei)(wei)(wei)(wei)(wei)(wei)細(xi)胞(bao)(bao)(bao)培(pei)養上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)，因(yin)(yin)該類樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)干(gan)擾因(yin)(yin)素(su)(su)(su)較多(duo)(duo)(duo)，如(ru)(ru)(ru)：細(xi)胞(bao)(bao)(bao)狀態、細(xi)胞(bao)(bao)(bao)數量(liang)(liang)(liang)(liang)(liang)(liang)、采樣(yang)(yang)(yang)(yang)(yang)(yang)時(shi)間(jian)(jian)等(deng)，所(suo)(suo)以(yi)(yi)(yi)(yi)可(ke)(ke)(ke)(ke)(ke)能(neng)存(cun)在(zai)(zai)(zai)(zai)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)不(bu)(bu)出的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情況。8.某(mou)些天然蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)或(huo)(huo)(huo)(huo)重(zhong)組(zu)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)，包(bao)括原(yuan)(yuan)核及(ji)(ji)(ji)(ji)(ji)真核重(zhong)組(zu)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)，可(ke)(ke)(ke)(ke)(ke)能(neng)因(yin)(yin)為(wei)(wei)(wei)(wei)(wei)(wei)與(yu)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)產品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)所(suo)(suo)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)及(ji)(ji)(ji)(ji)(ji)捕獲抗(kang)(kang)(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)不(bu)(bu)匹配(pei)，而不(bu)(bu)被(bei)(bei)(bei)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)出。 ELISA檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)承(cheng)諾公(gong)司(si)(si)長期(qi)(qi)(qi)與(yu)各大高(gao)(gao)(gao)校(xiao)、醫院及(ji)(ji)(ji)(ji)(ji)權威科(ke)(ke)研(yan)單位保(bao)(bao)持著(zhu)良(liang)好(hao)(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)合(he)(he)(he)(he)作(zuo)關(guan)(guan)系(xi)，公(gong)司(si)(si)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)及(ji)(ji)(ji)(ji)(ji)服(fu)(fu)(fu)務(wu)(wu)(wu)態度(du)(du)(du)(du)得(de)(de)到(dao)了(le)他們的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)致認(ren)可(ke)(ke)(ke)(ke)(ke)。篤(du)瑪生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)著(zhu)客戶(hu)至(zhi)(zhi)上(shang)(shang)(shang)(shang)(shang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)(yuan)則(ze)為(wei)(wei)(wei)(wei)(wei)(wei)客戶(hu)提供(gong)(gong)優(you)質(zhi)(zhi)(zhi)(zhi)產品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)，公(gong)司(si)(si)承(cheng)諾產品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)有(you)(you)(you)(you)任(ren)(ren)(ren)何(he)質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)問(wen)題(ti)免(mian)(mian)(mian)費包(bao)退(tui)換(huan)（非人(ren)為(wei)(wei)(wei)(wei)(wei)(wei)因(yin)(yin)素(su)(su)(su)造(zao)成(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)）。公(gong)司(si)(si)提供(gong)(gong)免(mian)(mian)(mian)費代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)服(fu)(fu)(fu)務(wu)(wu)(wu)，并(bing)(bing)(bing)且承(cheng)諾收到(dao)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)七個(ge)工(gong)(gong)作(zuo)日(ri)出結(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)(jie)果(guo)(guo)(guo)，確(que)保(bao)(bao)數據(ju)(ju)(ju)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)。   

 小(xiao)(xiao)鼠（Mouse）免疫(yi)球(qiu)蛋(dan)白(bai) G（IgG）ELISA 檢測(ce)(ce)(ce)(ce)試(shi)劑(ji)(ji)(ji)(ji)盒 檢測(ce)(ce)(ce)(ce)原(yuan)理(li)試(shi)劑(ji)(ji)(ji)(ji)盒采(cai)用(yong)(yong)雙抗(kang)體(ti)一步夾心法酶(mei)(mei)聯免疫(yi)吸(xi)附試(shi)驗（ELISA）。往 預先包被免疫(yi)球(qiu)蛋(dan)白(bai)M（IgM）抗(kang)體(ti)的(de)(de)(de)包被微(wei)孔(kong)(kong)(kong)中(zhong)(zhong)，依次(ci)(ci)(ci)加(jia)(jia)(jia)入(ru)標(biao)(biao)(biao)本(ben)(ben)(ben)、 標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)、HRP標(biao)(biao)(biao)記(ji)的(de)(de)(de)檢測(ce)(ce)(ce)(ce)抗(kang)體(ti)，經(jing)過溫(wen)(wen)(wen)育并(bing)徹(che)底洗(xi)(xi)(xi)(xi)滌(di)(di)(di)。用(yong)(yong)底物(wu)TMB顯 色，TMB在(zai)過氧化物(wu)酶(mei)(mei)的(de)(de)(de)催化下(xia)(xia)轉化成藍色，并(bing)在(zai)酸的(de)(de)(de)作(zuo)(zuo)(zuo)用(yong)(yong)下(xia)(xia)轉化成 ＊終(zhong)(zhong)的(de)(de)(de)黃色。顏色的(de)(de)(de)深(shen)淺和(he)(he)(he)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)免疫(yi)球(qiu)蛋(dan)白(bai)M（IgM）呈正相關(guan)。 用(yong)(yong)酶(mei)(mei)標(biao)(biao)(biao)儀在(zai)450nm 波長(chang)下(xia)(xia)測(ce)(ce)(ce)(ce)定吸(xi)光度(du)（OD 值），計算樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)濃(nong)度(du)。樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)收(shou)集(ji)、處理(li)及(ji)保(bao)存(cun)方法1. 血清(qing)：使(shi)(shi)(shi)(shi)用(yong)(yong)不(bu)(bu)(bu)含熱原(yuan)和(he)(he)(he)內(nei)毒(du)素(su)的(de)(de)(de)試(shi)管(guan)，操(cao)作(zuo)(zuo)(zuo)過程(cheng)中(zhong)(zhong)避(bi)(bi)免任(ren)何細(xi) 胞(bao)刺激，收(shou)集(ji)血液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)，3000 轉離心 10 分(fen)(fen)(fen)鐘(zhong)將血清(qing)和(he)(he)(he)紅細(xi)胞(bao)迅速小(xiao)(xiao)心 地(di)分(fen)(fen)(fen)離。 2. 血漿：EDTA、檸檬酸鹽或肝素(su)抗(kang)凝。3000 轉離心 30 分(fen)(fen)(fen)鐘(zhong)取(qu)上清(qing)。3. 細(xi)胞(bao)上清(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)：3000 轉離心 10 分(fen)(fen)(fen)鐘(zhong)去(qu)(qu)除(chu)顆粒和(he)(he)(he)聚合物(wu)。 4. 組織勻(yun)(yun)漿：將組織加(jia)(jia)(jia)入(ru)適量生(sheng)理(li)鹽水(shui)(shui)搗碎。3000 轉離心 10 分(fen)(fen)(fen)鐘(zhong) 取(qu)上清(qing)。5. 保(bao)存(cun)：如果(guo)(guo)(guo)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)收(shou)集(ji)后(hou)(hou)不(bu)(bu)(bu)及(ji)時檢測(ce)(ce)(ce)(ce)，請按(an)(an)一次(ci)(ci)(ci)用(yong)(yong)量分(fen)(fen)(fen)裝(zhuang)，凍(dong)存(cun) 于(yu)(yu)-20℃，避(bi)(bi)免反(fan)復凍(dong)融，在(zai)室溫(wen)(wen)(wen)下(xia)(xia)解凍(dong)并(bing)確保(bao)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)均勻(yun)(yun)地(di)充分(fen)(fen)(fen)解凍(dong)。自(zi)備物(wu)品(pin)(pin)(pin) 1. 酶(mei)(mei)標(biao)(biao)(biao)儀（450nm） 2. 高精度(du)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)器及(ji)槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL 3. 37℃恒溫(wen)(wen)(wen)箱(xiang)(xiang) 操(cao)作(zuo)(zuo)(zuo)注意事項(xiang) 1. 試(shi)劑(ji)(ji)(ji)(ji)盒保(bao)存(cun)在(zai) 2-8℃，使(shi)(shi)(shi)(shi)用(yong)(yong)前室溫(wen)(wen)(wen)平衡 20 分(fen)(fen)(fen)鐘(zhong)。從(cong)冰箱(xiang)(xiang)取(qu)出(chu)(chu)(chu)的(de)(de)(de) 濃(nong)縮洗(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)會有(you)結(jie)晶(jing)，這(zhe)屬于(yu)(yu)正常(chang)現(xian)象，水(shui)(shui)浴(yu)(yu)加(jia)(jia)(jia)熱使(shi)(shi)(shi)(shi)結(jie)晶(jing)完全(quan)溶(rong)解 后(hou)(hou)再(zai)使(shi)(shi)(shi)(shi)用(yong)(yong)。2. 實(shi)(shi)驗中(zhong)(zhong)不(bu)(bu)(bu)用(yong)(yong)的(de)(de)(de)板(ban)(ban)(ban)條(tiao)應立(li)即放(fang)回自(zi)封袋中(zhong)(zhong)，密封（低溫(wen)(wen)(wen)干(gan)燥）保(bao) 存(cun)。3. 濃(nong)度(du)為(wei)(wei) 0 的(de)(de)(de) S0 號(hao)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)即可視(shi)為(wei)(wei)陰性(xing)(xing)(xing)對照或者空(kong)白(bai)；按(an)(an)照說(shuo)明(ming)(ming) 書操(cao)作(zuo)(zuo)(zuo)時樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)已經(jing)稀(xi)(xi)釋(shi)(shi) 5 倍，＊終(zhong)(zhong)結(jie)果(guo)(guo)(guo)乘以(yi) 5 才是樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)實(shi)(shi)際濃(nong)度(du)。4. 嚴(yan)(yan)格按(an)(an)照說(shuo)明(ming)(ming)書中(zhong)(zhong)標(biao)(biao)(biao)明(ming)(ming)的(de)(de)(de)時間、加(jia)(jia)(jia)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)量及(ji)順(shun)序進行(xing)溫(wen)(wen)(wen)育操(cao)作(zuo)(zuo)(zuo)。5. 所(suo)有(you)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)組分(fen)(fen)(fen)使(shi)(shi)(shi)(shi)用(yong)(yong)前充分(fen)(fen)(fen)搖勻(yun)(yun)。 試(shi)劑(ji)(ji)(ji)(ji)盒組成 名(ming)稱96孔(kong)(kong)(kong)配置(zhi)(zhi)48孔(kong)(kong)(kong)配置(zhi)(zhi)備注微(wei)孔(kong)(kong)(kong)酶(mei)(mei)標(biao)(biao)(biao)板(ban)(ban)(ban) 12 孔(kong)(kong)(kong)×8 條(tiao)12 孔(kong)(kong)(kong)×4 條(tiao)無(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)0.3ml*6管(guan)0.3ml*6管(guan)無(wu)(wu)(wu)(wu)(wu)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)稀(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)6ml3ml無(wu)(wu)(wu)(wu)(wu)檢測(ce)(ce)(ce)(ce)抗(kang)體(ti)-HRP10ml5ml無(wu)(wu)(wu)(wu)(wu)20×洗(xi)(xi)(xi)(xi)滌(di)(di)(di)緩(huan)(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)25mL15ml按(an)(an)說(shuo)明(ming)(ming)書進行(xing)稀(xi)(xi)釋(shi)(shi)底物(wu)A6ml3ml無(wu)(wu)(wu)(wu)(wu)底物(wu)B6ml 3ml無(wu)(wu)(wu)(wu)(wu)終(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)6ml3ml無(wu)(wu)(wu)(wu)(wu)封板(ban)(ban)(ban)膜(mo)2張2張無(wu)(wu)(wu)(wu)(wu)說(shuo)明(ming)(ming)書1份1份無(wu)(wu)(wu)(wu)(wu)自(zi)封袋1個(ge)1個(ge)無(wu)(wu)(wu)(wu)(wu)注：標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)（S0-S5）濃(nong)度(du)依次(ci)(ci)(ci)為(wei)(wei)：0、1.25、2.5、5、10、20 g/L 試(shi)劑(ji)(ji)(ji)(ji)的(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)備 20×洗(xi)(xi)(xi)(xi)滌(di)(di)(di)緩(huan)(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)稀(xi)(xi)釋(shi)(shi)：蒸餾(liu)水(shui)(shui)按(an)(an) 1：20 稀(xi)(xi)釋(shi)(shi)，即 1 份的(de)(de)(de) 20×洗(xi)(xi)(xi)(xi)滌(di)(di)(di) 緩(huan)(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)加(jia)(jia)(jia) 19 份的(de)(de)(de)蒸餾(liu)水(shui)(shui)。 洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)方法 1. 手工(gong)(gong)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)：甩(shuai)盡孔(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，每(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)滿洗(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，靜(jing)置(zhi)(zhi) 1min 后(hou)(hou)甩(shuai)盡 孔(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，在(zai)吸(xi)水(shui)(shui)紙上拍干(gan)，如此(ci)(ci)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban) 5 次(ci)(ci)(ci)。 2. 自(zi)動洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)機：每(mei)孔(kong)(kong)(kong)注入(ru)洗(xi)(xi)(xi)(xi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 350μL，浸泡 1min，洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban) 5 次(ci)(ci)(ci)。 操(cao)作(zuo)(zuo)(zuo)步驟 1. 從(cong)室溫(wen)(wen)(wen)平衡 20min 后(hou)(hou)的(de)(de)(de)鋁箔袋中(zhong)(zhong)取(qu)出(chu)(chu)(chu)所(suo)需(xu)板(ban)(ban)(ban)條(tiao)，剩余板(ban)(ban)(ban)條(tiao)用(yong)(yong)自(zi) 封袋密封放(fang)回 4℃。 2. 設置(zhi)(zhi)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)和(he)(he)(he)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)，標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)各加(jia)(jia)(jia)不(bu)(bu)(bu)同濃(nong)度(du)的(de)(de)(de)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin) 50μ L； 3. 樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)先加(jia)(jia)(jia)待(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben) 10μL，再(zai)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)稀(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 40μL；空(kong)白(bai)孔(kong)(kong)(kong)不(bu)(bu)(bu) 加(jia)(jia)(jia)。 4. 除(chu)空(kong)白(bai)孔(kong)(kong)(kong)外，標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)和(he)(he)(he)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)中(zhong)(zhong)每(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)入(ru)辣根過氧化物(wu)酶(mei)(mei) （HRP）標(biao)(biao)(biao)記(ji)的(de)(de)(de)檢測(ce)(ce)(ce)(ce)抗(kang)體(ti) 100μL，用(yong)(yong)封板(ban)(ban)(ban)膜(mo)封住反(fan)應孔(kong)(kong)(kong)，37℃水(shui)(shui)浴(yu)(yu)鍋 或恒溫(wen)(wen)(wen)箱(xiang)(xiang)溫(wen)(wen)(wen)育 60min。 5. 棄去(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)，吸(xi)水(shui)(shui)紙上拍干(gan)，每(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)滿洗(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，靜(jing)置(zhi)(zhi) 1min，甩(shuai)去(qu)(qu) 洗(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)，吸(xi)水(shui)(shui)紙上拍干(gan)，如此(ci)(ci)重復洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban) 5 次(ci)(ci)(ci)（也可用(yong)(yong)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)機洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)）。 6. 每(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)入(ru)底物(wu) A、B 各 50μL，37℃避(bi)(bi)光孵育 15min。 7. 每(mei)孔(kong)(kong)(kong)加(jia)(jia)(jia)入(ru)終(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 50μL，15min 內(nei)，在(zai) 450nm 波長(chang)處測(ce)(ce)(ce)(ce)定各孔(kong)(kong)(kong)的(de)(de)(de) OD 值。 結(jie)果(guo)(guo)(guo)判斷(duan)(duan) 繪制(zhi)(zhi)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲線(xian)：在(zai) Excel 工(gong)(gong)作(zuo)(zuo)(zuo)表中(zhong)(zhong)，以(yi)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)濃(nong)度(du)作(zuo)(zuo)(zuo)橫(heng)坐(zuo)標(biao)(biao)(biao)，對應 OD 值作(zuo)(zuo)(zuo)縱坐(zuo)標(biao)(biao)(biao)，繪制(zhi)(zhi)出(chu)(chu)(chu)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)線(xian)性(xing)(xing)(xing)回歸(gui)曲線(xian)，按(an)(an)曲線(xian)方程(cheng)計算各樣(yang)(yang)(yang)(yang) 本(ben)(ben)(ben)濃(nong)度(du)值。 試(shi)劑(ji)(ji)(ji)(ji)盒性(xing)(xing)(xing)能 1. 準(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)(xing)：標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)線(xian)性(xing)(xing)(xing)回歸(gui)與預期濃(nong)度(du)相關(guan)系數(shu)(shu) R 值，大于(yu)(yu)等于(yu)(yu) 0.9900。 2. 靈(ling)敏度(du)：＊低檢測(ce)(ce)(ce)(ce)濃(nong)度(du)小(xiao)(xiao)于(yu)(yu) 0.1 g/L。 3. 特(te)異性(xing)(xing)(xing)：不(bu)(bu)(bu)與其(qi)它可溶(rong)性(xing)(xing)(xing)結(jie)構類(lei)似(si)物(wu)交叉(cha)反(fan)應。 4. 重復性(xing)(xing)(xing)：板(ban)(ban)(ban)內(nei)、板(ban)(ban)(ban)間變(bian)異系數(shu)(shu)均小(xiao)(xiao)于(yu)(yu) 15%。5. 貯藏：2-8℃，避(bi)(bi)光防潮保(bao)存(cun)。6. 有(you)效期：6 個(ge)月(yue) 免責聲(sheng)明(ming)(ming) 1. 試(shi)劑(ji)(ji)(ji)(ji)盒僅(jin)供研究(jiu)使(shi)(shi)(shi)(shi)用(yong)(yong)，不(bu)(bu)(bu)得(de)用(yong)(yong)于(yu)(yu)臨床實(shi)(shi)驗或人(ren)體(ti)實(shi)(shi)驗，否(fou)則所(suo) 產生(sheng)的(de)(de)(de)一切后(hou)(hou)果(guo)(guo)(guo)，由(you)實(shi)(shi)驗者承(cheng)擔(dan)，本(ben)(ben)(ben)公司(si)概(gai)不(bu)(bu)(bu)負責。 2. 嚴(yan)(yan)格按(an)(an)照說(shuo)明(ming)(ming)書操(cao)作(zuo)(zuo)(zuo)，實(shi)(shi)驗者違(wei)反(fan)說(shuo)明(ming)(ming)書操(cao)作(zuo)(zuo)(zuo)，后(hou)(hou)果(guo)(guo)(guo)由(you)實(shi)(shi)驗者承(cheng)擔(dan)。本(ben)(ben)(ben)試(shi)劑(ji)(ji)(ji)(ji)盒只能用(yong)(yong)于(yu)(yu)科學研究(jiu)，不(bu)(bu)(bu)得(de)用(yong)(yong)于(yu)(yu)醫學診斷(duan)(duan)

 小(xiao)鼠（Mouse）抗(kang)(kang)(kang)雙鏈 DNA 抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)/天(tian)然(ran) DNA 抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)（dsDNA） ELISA 檢測(ce)試(shi)(shi)劑(ji)(ji)(ji)盒(he) 檢測(ce)原(yuan)理試(shi)(shi)劑(ji)(ji)(ji)盒(he)采用(yong)(yong)(yong)雙抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)一(yi)(yi)步夾心(xin)法(fa)酶(mei)聯免(mian)疫(yi)吸附試(shi)(shi)驗(yan)（ELISA）。往 預(yu)先包(bao)被免(mian)疫(yi)球(qiu)蛋白M（IgM）抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)包(bao)被微孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，依次(ci)加(jia)(jia)(jia)(jia)入(ru)標(biao)(biao)(biao)(biao)本(ben)(ben)(ben)、 標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)、HRP標(biao)(biao)(biao)(biao)記的(de)(de)(de)檢測(ce)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)，經過(guo)溫(wen)(wen)(wen)育并(bing)(bing)徹底(di)洗(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)。用(yong)(yong)(yong)底(di)物(wu)(wu)TMB顯 色，TMB在(zai)(zai)過(guo)氧(yang)化物(wu)(wu)酶(mei)的(de)(de)(de)催化下(xia)(xia)轉化成藍色，并(bing)(bing)在(zai)(zai)酸(suan)的(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)下(xia)(xia)轉化成 ＊終(zhong)的(de)(de)(de)黃色。顏色的(de)(de)(de)深淺(qian)和(he)(he)(he)樣(yang)(yang)品(pin)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)免(mian)疫(yi)球(qiu)蛋白M（IgM）呈(cheng)正(zheng)相關。 用(yong)(yong)(yong)酶(mei)標(biao)(biao)(biao)(biao)儀在(zai)(zai)450nm 波長(chang)下(xia)(xia)測(ce)定吸光(guang)(guang)度(du)(du)(du)（OD 值），計算樣(yang)(yang)品(pin)濃度(du)(du)(du)。樣(yang)(yang)品(pin)收(shou)(shou)集、處理及保(bao)存(cun)方法(fa)1. 血(xue)(xue)清：使用(yong)(yong)(yong)不(bu)含(han)熱原(yuan)和(he)(he)(he)內(nei)毒素的(de)(de)(de)試(shi)(shi)管(guan)(guan)，操(cao)作(zuo)(zuo)(zuo)(zuo)過(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)避(bi)(bi)免(mian)任何細(xi) 胞刺(ci)激，收(shou)(shou)集血(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)后(hou)，3000 轉離心(xin) 10 分(fen)(fen)(fen)鐘將血(xue)(xue)清和(he)(he)(he)紅(hong)細(xi)胞迅(xun)速(su)小(xiao)心(xin) 地分(fen)(fen)(fen)離。 2. 血(xue)(xue)漿：EDTA、檸(ning)檬酸(suan)鹽或(huo)肝素抗(kang)(kang)(kang)凝。3000 轉離心(xin) 30 分(fen)(fen)(fen)鐘取上(shang)清。3. 細(xi)胞上(shang)清液(ye)(ye)(ye)(ye)(ye)(ye)：3000 轉離心(xin) 10 分(fen)(fen)(fen)鐘去除(chu)顆(ke)粒和(he)(he)(he)聚合物(wu)(wu)。 4. 組(zu)織(zhi)勻漿：將組(zu)織(zhi)加(jia)(jia)(jia)(jia)入(ru)適量生理鹽水(shui)搗碎。3000 轉離心(xin) 10 分(fen)(fen)(fen)鐘 取上(shang)清。5. 保(bao)存(cun)：如(ru)(ru)(ru)果樣(yang)(yang)本(ben)(ben)(ben)收(shou)(shou)集后(hou)不(bu)及時(shi)檢測(ce)，請(qing)按(an)一(yi)(yi)次(ci)用(yong)(yong)(yong)量分(fen)(fen)(fen)裝，凍(dong)(dong)存(cun) 于(yu)(yu)(yu)(yu)-20℃，避(bi)(bi)免(mian)反復凍(dong)(dong)融，在(zai)(zai)室溫(wen)(wen)(wen)下(xia)(xia)解(jie)(jie)凍(dong)(dong)并(bing)(bing)確保(bao)樣(yang)(yang)品(pin)均(jun)勻地充分(fen)(fen)(fen)解(jie)(jie)凍(dong)(dong)。自備物(wu)(wu)品(pin) 1. 酶(mei)標(biao)(biao)(biao)(biao)儀（450nm） 2. 高(gao)精(jing)度(du)(du)(du)加(jia)(jia)(jia)(jia)樣(yang)(yang)器及槍(qiang)頭：0.5-10uL、2-20uL、20-200uL、200-1000uL 3. 37℃恒(heng)(heng)溫(wen)(wen)(wen)箱(xiang)(xiang) 操(cao)作(zuo)(zuo)(zuo)(zuo)注意事項(xiang) 1. 試(shi)(shi)劑(ji)(ji)(ji)盒(he)保(bao)存(cun)在(zai)(zai) 2-8℃，使用(yong)(yong)(yong)前室溫(wen)(wen)(wen)平(ping)衡 20 分(fen)(fen)(fen)鐘。從(cong)冰箱(xiang)(xiang)取出(chu)的(de)(de)(de) 濃縮洗(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)會有結(jie)晶(jing)，這(zhe)屬于(yu)(yu)(yu)(yu)正(zheng)常(chang)現(xian)象，水(shui)浴加(jia)(jia)(jia)(jia)熱使結(jie)晶(jing)完全溶(rong)解(jie)(jie) 后(hou)再使用(yong)(yong)(yong)。2. 實(shi)驗(yan)中(zhong)(zhong)(zhong)(zhong)(zhong)不(bu)用(yong)(yong)(yong)的(de)(de)(de)板(ban)(ban)(ban)(ban)條應立(li)即放回(hui)自封(feng)(feng)(feng)袋(dai)中(zhong)(zhong)(zhong)(zhong)(zhong)，密封(feng)(feng)(feng)（低(di)溫(wen)(wen)(wen)干燥）保(bao) 存(cun)。3. 濃度(du)(du)(du)為 0 的(de)(de)(de) S0 號標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)即可視為陰性(xing)(xing)(xing)對照(zhao)(zhao)或(huo)者(zhe)空(kong)白；按(an)照(zhao)(zhao)說(shuo)(shuo)明(ming) 書(shu)操(cao)作(zuo)(zuo)(zuo)(zuo)時(shi)樣(yang)(yang)本(ben)(ben)(ben)已經稀(xi)(xi)釋(shi) 5 倍，＊終(zhong)結(jie)果乘以(yi) 5 才(cai)是樣(yang)(yang)本(ben)(ben)(ben)實(shi)際(ji)濃度(du)(du)(du)。4. 嚴格按(an)照(zhao)(zhao)說(shuo)(shuo)明(ming)書(shu)中(zhong)(zhong)(zhong)(zhong)(zhong)標(biao)(biao)(biao)(biao)明(ming)的(de)(de)(de)時(shi)間(jian)、加(jia)(jia)(jia)(jia)液(ye)(ye)(ye)(ye)(ye)(ye)量及順序進行(xing)溫(wen)(wen)(wen)育操(cao)作(zuo)(zuo)(zuo)(zuo)。5. 所有液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)組(zu)分(fen)(fen)(fen)使用(yong)(yong)(yong)前充分(fen)(fen)(fen)搖勻。 試(shi)(shi)劑(ji)(ji)(ji)盒(he)組(zu)成 名稱96孔(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)(zhi)48孔(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)(zhi)備注微孔(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)標(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban) 12 孔(kong)(kong)(kong)(kong)(kong)(kong)×8 條12 孔(kong)(kong)(kong)(kong)(kong)(kong)×4 條無(wu)(wu)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)0.3ml*6管(guan)(guan)0.3ml*6管(guan)(guan)無(wu)(wu)樣(yang)(yang)本(ben)(ben)(ben)稀(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)(ye)(ye)6ml3ml無(wu)(wu)檢測(ce)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)-HRP10ml5ml無(wu)(wu)20×洗(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)緩(huan)沖液(ye)(ye)(ye)(ye)(ye)(ye)25mL15ml按(an)說(shuo)(shuo)明(ming)書(shu)進行(xing)稀(xi)(xi)釋(shi)底(di)物(wu)(wu)A6ml3ml無(wu)(wu)底(di)物(wu)(wu)B6ml 3ml無(wu)(wu)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)6ml3ml無(wu)(wu)封(feng)(feng)(feng)板(ban)(ban)(ban)(ban)膜2張(zhang)2張(zhang)無(wu)(wu)說(shuo)(shuo)明(ming)書(shu)1份1份無(wu)(wu)自封(feng)(feng)(feng)袋(dai)1個1個無(wu)(wu)注：標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)（S0-S5）濃度(du)(du)(du)依次(ci)為：0、1.25、2.5、5、10、20 g/L 試(shi)(shi)劑(ji)(ji)(ji)的(de)(de)(de)準(zhun)(zhun)(zhun)備 20×洗(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)緩(huan)沖液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)稀(xi)(xi)釋(shi)：蒸餾(liu)(liu)水(shui)按(an) 1：20 稀(xi)(xi)釋(shi)，即 1 份的(de)(de)(de) 20×洗(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di) 緩(huan)沖液(ye)(ye)(ye)(ye)(ye)(ye)加(jia)(jia)(jia)(jia) 19 份的(de)(de)(de)蒸餾(liu)(liu)水(shui)。 洗(xi)(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)方法(fa) 1. 手工洗(xi)(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)：甩盡孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿(man)洗(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)，靜(jing)置(zhi)(zhi) 1min 后(hou)甩盡 孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，在(zai)(zai)吸水(shui)紙(zhi)上(shang)拍(pai)干，如(ru)(ru)(ru)此(ci)洗(xi)(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban) 5 次(ci)。 2. 自動洗(xi)(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)機(ji)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)注入(ru)洗(xi)(xi)(xi)(xi)(xi)(xi)液(ye)(ye)(ye)(ye)(ye)(ye) 350μL，浸泡 1min，洗(xi)(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban) 5 次(ci)。 操(cao)作(zuo)(zuo)(zuo)(zuo)步驟 1. 從(cong)室溫(wen)(wen)(wen)平(ping)衡 20min 后(hou)的(de)(de)(de)鋁箔袋(dai)中(zhong)(zhong)(zhong)(zhong)(zhong)取出(chu)所需(xu)板(ban)(ban)(ban)(ban)條，剩余板(ban)(ban)(ban)(ban)條用(yong)(yong)(yong)自 封(feng)(feng)(feng)袋(dai)密封(feng)(feng)(feng)放回(hui) 4℃。 2. 設置(zhi)(zhi)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)(he)樣(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)，標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)各(ge)加(jia)(jia)(jia)(jia)不(bu)同濃度(du)(du)(du)的(de)(de)(de)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin) 50μ L； 3. 樣(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)待測(ce)樣(yang)(yang)本(ben)(ben)(ben) 10μL，再加(jia)(jia)(jia)(jia)樣(yang)(yang)本(ben)(ben)(ben)稀(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)(ye)(ye) 40μL；空(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)不(bu) 加(jia)(jia)(jia)(jia)。 4. 除(chu)空(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)外，標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)(he)樣(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)辣根過(guo)氧(yang)化物(wu)(wu)酶(mei) （HRP）標(biao)(biao)(biao)(biao)記的(de)(de)(de)檢測(ce)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti) 100μL，用(yong)(yong)(yong)封(feng)(feng)(feng)板(ban)(ban)(ban)(ban)膜封(feng)(feng)(feng)住(zhu)反應孔(kong)(kong)(kong)(kong)(kong)(kong)，37℃水(shui)浴鍋(guo) 或(huo)恒(heng)(heng)溫(wen)(wen)(wen)箱(xiang)(xiang)溫(wen)(wen)(wen)育 60min。 5. 棄(qi)去液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，吸水(shui)紙(zhi)上(shang)拍(pai)干，每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿(man)洗(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)，靜(jing)置(zhi)(zhi) 1min，甩去 洗(xi)(xi)(xi)(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)(ye)(ye)，吸水(shui)紙(zhi)上(shang)拍(pai)干，如(ru)(ru)(ru)此(ci)重(zhong)復洗(xi)(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban) 5 次(ci)（也(ye)可用(yong)(yong)(yong)洗(xi)(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)機(ji)洗(xi)(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)）。 6. 每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)底(di)物(wu)(wu) A、B 各(ge) 50μL，37℃避(bi)(bi)光(guang)(guang)孵育 15min。 7. 每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)終(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye) 50μL，15min 內(nei)，在(zai)(zai) 450nm 波長(chang)處測(ce)定各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de) OD 值。 結(jie)果判斷(duan) 繪(hui)制標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲線：在(zai)(zai) Excel 工作(zuo)(zuo)(zuo)(zuo)表中(zhong)(zhong)(zhong)(zhong)(zhong)，以(yi)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)濃度(du)(du)(du)作(zuo)(zuo)(zuo)(zuo)橫坐標(biao)(biao)(biao)(biao)，對應 OD 值作(zuo)(zuo)(zuo)(zuo)縱坐標(biao)(biao)(biao)(biao)，繪(hui)制出(chu)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)線性(xing)(xing)(xing)回(hui)歸曲線，按(an)曲線方程計算各(ge)樣(yang)(yang) 本(ben)(ben)(ben)濃度(du)(du)(du)值。 試(shi)(shi)劑(ji)(ji)(ji)盒(he)性(xing)(xing)(xing)能 1. 準(zhun)(zhun)(zhun)確性(xing)(xing)(xing)：標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)線性(xing)(xing)(xing)回(hui)歸與(yu)預(yu)期(qi)濃度(du)(du)(du)相關系(xi)數(shu) R 值，大于(yu)(yu)(yu)(yu)等(deng)于(yu)(yu)(yu)(yu) 0.9900。 2. 靈敏度(du)(du)(du)：＊低(di)檢測(ce)濃度(du)(du)(du)小(xiao)于(yu)(yu)(yu)(yu) 0.1 g/L。 3. 特異性(xing)(xing)(xing)：不(bu)與(yu)其它可溶(rong)性(xing)(xing)(xing)結(jie)構類似物(wu)(wu)交叉反應。 4. 重(zhong)復性(xing)(xing)(xing)：板(ban)(ban)(ban)(ban)內(nei)、板(ban)(ban)(ban)(ban)間(jian)變異系(xi)數(shu)均(jun)小(xiao)于(yu)(yu)(yu)(yu) 15%。5. 貯藏：2-8℃，避(bi)(bi)光(guang)(guang)防潮保(bao)存(cun)。6. 有效期(qi)：6 個月 免(mian)責(ze)(ze)聲明(ming) 1. 試(shi)(shi)劑(ji)(ji)(ji)盒(he)僅(jin)供研究使用(yong)(yong)(yong)，不(bu)得(de)用(yong)(yong)(yong)于(yu)(yu)(yu)(yu)臨床(chuang)實(shi)驗(yan)或(huo)人體(ti)(ti)(ti)(ti)實(shi)驗(yan)，否則所 產生的(de)(de)(de)一(yi)(yi)切后(hou)果，由實(shi)驗(yan)者(zhe)承(cheng)擔(dan)，本(ben)(ben)(ben)公(gong)司概(gai)不(bu)負責(ze)(ze)。 2. 嚴格按(an)照(zhao)(zhao)說(shuo)(shuo)明(ming)書(shu)操(cao)作(zuo)(zuo)(zuo)(zuo)，實(shi)驗(yan)者(zhe)違反說(shuo)(shuo)明(ming)書(shu)操(cao)作(zuo)(zuo)(zuo)(zuo)，后(hou)果由實(shi)驗(yan)者(zhe)承(cheng)擔(dan)。本(ben)(ben)(ben)試(shi)(shi)劑(ji)(ji)(ji)盒(he)只能用(yong)(yong)(yong)于(yu)(yu)(yu)(yu)科學研究，不(bu)得(de)用(yong)(yong)(yong)于(yu)(yu)(yu)(yu)醫(yi)學診斷(duan)