實(shi)驗(yan)原理 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)采(cai)用(yong)(yong)(yong)雙抗(kang)(kang)(kang)(kang)體(ti)(ti)一步夾心(xin)法酶(mei)(mei)(mei)(mei)聯免疫(yi)吸(xi)(xi)附(fu)(fu)(fu)試(shi)(shi)(shi)(shi)(shi)驗(yan)（ELISA）。往預先(xian)包被牛(niu)(niu)血(xue)(xue)(xue)(xue)管(guan)內(nei)(nei)(nei)(nei)(nei)皮(pi)(pi)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)粘(zhan)(zhan)附(fu)(fu)(fu)分(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)(zi)(zi)1(VCAM-1/CD106) 捕獲抗(kang)(kang)(kang)(kang)體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)中(zhong)，依(yi)(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)(kang)體(ti)(ti)，經過(guo)(guo)(guo)(guo)(guo)溫育(yu)(yu)并(bing)徹底(di)(di)洗(xi)(xi)滌(di)(di)。用(yong)(yong)(yong)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯(xian)色(se)(se)(se)，TMB在(zai)(zai)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化下(xia)(xia)轉(zhuan)化成(cheng)藍色(se)(se)(se)，并(bing)在(zai)(zai)酸(suan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)用(yong)(yong)(yong)下(xia)(xia)轉(zhuan)化成(cheng)＊終(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)(huang)色(se)(se)(se)。顏色(se)(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)牛(niu)(niu)血(xue)(xue)(xue)(xue)管(guan)內(nei)(nei)(nei)(nei)(nei)皮(pi)(pi)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)粘(zhan)(zhan)附(fu)(fu)(fu)分(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)(zi)(zi)1(VCAM-1/CD106) 呈正相(xiang)(xiang)(xiang)關。用(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)450nm 波(bo)長下(xia)(xia)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定吸(xi)(xi)光(guang)度(du)(du)(du)（OD 值(zhi)(zhi)(zhi)(zhi)），計(ji)算(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)。保(bao)(bao)存(cun)(cun)(cun)條件及(ji)(ji)有(you)(you)效(xiao)(xiao)期(qi)1.本(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)用(yong)(yong)(yong)于體(ti)(ti)外定量(liang)(liang)(liang)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)血(xue)(xue)(xue)(xue)清(qing)(qing)、血(xue)(xue)(xue)(xue)漿、組織(zhi)勻漿及(ji)(ji)相(xiang)(xiang)(xiang)關液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)牛(niu)(niu)血(xue)(xue)(xue)(xue)管(guan)內(nei)(nei)(nei)(nei)(nei)皮(pi)(pi)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)粘(zhan)(zhan)附(fu)(fu)(fu)分(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)(zi)(zi)1(VCAM-1/CD106) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)量(liang)(liang)(liang)；2.試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)保(bao)(bao)存(cun)(cun)(cun)：2-8℃；3.有(you)(you)效(xiao)(xiao)期(qi)：6個月(yue)。牛(niu)(niu)血(xue)(xue)(xue)(xue)管(guan)內(nei)(nei)(nei)(nei)(nei)皮(pi)(pi)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)粘(zhan)(zhan)附(fu)(fu)(fu)分(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)(zi)(zi)1(VCAM-1/CD106)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集及(ji)(ji)保(bao)(bao)存(cun)(cun)(cun)1.血(xue)(xue)(xue)(xue)清(qing)(qing)：全血(xue)(xue)(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)請于室溫放(fang)(fang)置(zhi)(zhi)2小(xiao)時(shi)(shi)(shi)(shi)或(huo)(huo)(huo)(huo)4℃過(guo)(guo)(guo)(guo)(guo)一晚后(hou)(hou)(hou)于1000 x g離(li)(li)心(xin)20分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，取(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)即(ji)(ji)可(ke)(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)放(fang)(fang)于-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)(cun)(cun)，但應(ying)(ying)(ying)避免反(fan)復凍(dong)(dong)融(rong)。 2.血(xue)(xue)(xue)(xue)漿：可(ke)(ke)(ke)用(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)肝素(su)(su)(su)(su)(su)(su)作(zuo)為(wei)(wei)抗(kang)(kang)(kang)(kang)凝(ning)劑(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)采(cai)集后(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)于2 - 8° C 1000 x g離(li)(li)心(xin)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)放(fang)(fang)于-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)(cun)(cun)，但應(ying)(ying)(ying)避免反(fan)復凍(dong)(dong)融(rong)。 3.細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)培(pei)養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)(shang)清(qing)(qing)或(huo)(huo)(huo)(huo)其它生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)：1000 x g離(li)(li)心(xin)20分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，取(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)即(ji)(ji)可(ke)(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)放(fang)(fang)于-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)存(cun)(cun)(cun)，但應(ying)(ying)(ying)避免反(fan)復凍(dong)(dong)融(rong)。 (注：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)溶(rong)血(xue)(xue)(xue)(xue)會影響**檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)果，因此(ci)(ci)溶(rong)血(xue)(xue)(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)不(bu)(bu)(bu)宜進(jin)行此(ci)(ci)項(xiang)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。) 牛(niu)(niu)血(xue)(xue)(xue)(xue)管(guan)內(nei)(nei)(nei)(nei)(nei)皮(pi)(pi)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)粘(zhan)(zhan)附(fu)(fu)(fu)分(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)(zi)(zi)1(VCAM-1/CD106)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he) 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則：首先(xian)通過(guo)(guo)(guo)(guo)(guo)文(wen)獻檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)索(suo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)(fang)式(shi)(shi)(shi)了解待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含(han)量(liang)(liang)(liang)，確定適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)(shu)。只(zhi)有(you)(you)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍內(nei)(nei)(nei)(nei)(nei)，檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)(jie)果才是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)中(zhong)，應(ying)(ying)(ying)做(zuo)(zuo)(zuo)好(hao)詳細(xi)(xi)(xi)(xi)(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)錄。計(ji)算(suan)(suan)濃(nong)(nong)(nong)(nong)度(du)(du)(du)時(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)應(ying)(ying)(ying)再(zai)乘(cheng)以(yi)(yi)(yi)“N”。 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則：2瓶，每(mei)(mei)(mei)瓶臨用(yong)(yong)(yong)前(qian)(qian)(qian)(qian)(qian)以(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)1ml，蓋好(hao)后(hou)(hou)(hou)靜置(zhi)(zhi)10分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)上(shang)(shang)(shang)(shang)，然后(hou)(hou)(hou)反(fan)復顛倒/搓動以(yi)(yi)(yi)助溶(rong)解，其濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)300 pg/ml，做(zuo)(zuo)(zuo)系(xi)列倍(bei)比稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)直(zhi)接(jie)作(zuo)為(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)度(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)前(qian)(qian)(qian)(qian)(qian)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)制(zhi)(zhi)(zhi)。 如(ru)(ru)(ru)配(pei)(pei)制(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)：取(qu)(qu)(qu)0.5ml（不(bu)(bu)(bu)要(yao)少(shao)(shao)于0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)含(han)0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)中(zhong)，混(hun)(hun)勻即(ji)(ji)可(ke)(ke)(ke)，其余濃(nong)(nong)(nong)(nong)度(du)(du)(du)以(yi)(yi)(yi)此(ci)(ci)類推(tui)。 生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則：臨用(yong)(yong)(yong)前(qian)(qian)(qian)(qian)(qian)以(yi)(yi)(yi)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)(qian)根據(ju)預先(xian)計(ji)算(suan)(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)實(shi)驗(yan)所(suo)(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)配(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)100μl），實(shi)際(ji)配(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)10μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例配(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)(hun)勻，在(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)前(qian)(qian)(qian)(qian)(qian)一小(xiao)時(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)制(zhi)(zhi)(zhi)。 辣(la)(la)(la)根過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則：臨用(yong)(yong)(yong)前(qian)(qian)(qian)(qian)(qian)以(yi)(yi)(yi)辣(la)(la)(la)根過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)(qian)根據(ju)預先(xian)計(ji)算(suan)(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)實(shi)驗(yan)所(suo)(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)配(pei)(pei)制(zhi)(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)100μl），實(shi)際(ji)配(pei)(pei)制(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)制(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)10μl辣(la)(la)(la)根過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)(su)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl辣(la)(la)(la)根過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例配(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)(hun)勻，在(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)前(qian)(qian)(qian)(qian)(qian)一小(xiao)時(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)制(zhi)(zhi)(zhi)。 牛(niu)(niu)血(xue)(xue)(xue)(xue)管(guan)內(nei)(nei)(nei)(nei)(nei)皮(pi)(pi)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)粘(zhan)(zhan)附(fu)(fu)(fu)分(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)(zi)(zi)1(VCAM-1/CD106)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)操作(zuo)步驟(zou) 實(shi)驗(yan)開始前(qian)(qian)(qian)(qian)(qian)，請提前(qian)(qian)(qian)(qian)(qian)配(pei)(pei)置(zhi)(zhi)好(hao)所(suo)(suo)有(you)(you)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)或(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)，均需(xu)混(hun)(hun)勻，混(hun)(hun)勻時(shi)(shi)(shi)(shi)盡量(liang)(liang)(liang)避免起泡(pao)。每(mei)(mei)(mei)次(ci)(ci)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)該(gai)做(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)。如(ru)(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)過(guo)(guo)(guo)(guo)(guo)高時(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)進(jin)行稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)使(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)符(fu)合試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范圍。 1.加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)設空白(bai)孔(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)、待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)。空白(bai)孔(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)100μl，注意不(bu)(bu)(bu)要(yao)有(you)(you)氣泡(pao)，加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)于酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)底(di)(di)部(bu)，盡量(liang)(liang)(liang)不(bu)(bu)(bu)觸(chu)及(ji)(ji)孔(kong)壁(bi)，輕(qing)輕(qing)晃動混(hun)(hun)勻，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)(shang)蓋或(huo)(huo)(huo)(huo)覆膜，37℃反(fan)應(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。 為(wei)(wei)保(bao)(bao)證實(shi)驗(yan)結(jie)(jie)果有(you)(you)效(xiao)(xiao)性(xing)(xing)，每(mei)(mei)(mei)次(ci)(ci)實(shi)驗(yan)請使(shi)(shi)用(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)液(ye)(ye)(ye)(ye)(ye)。 2.棄(qi)(qi)去(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，甩(shuai)干(gan)，不(bu)(bu)(bu)用(yong)(yong)(yong)洗(xi)(xi)滌(di)(di)。每(mei)(mei)(mei)孔(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye) 100μl（取(qu)(qu)(qu)1μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例配(pei)(pei)制(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)(hun)勻，在(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)前(qian)(qian)(qian)(qian)(qian)一小(xiao)時(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)制(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。 3.溫育(yu)(yu)60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)(hou)，棄(qi)(qi)去(qu)孔(kong)內(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，甩(shuai)干(gan)，洗(xi)(xi)板(ban)(ban)3次(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)浸(jin)泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)，甩(shuai)干(gan)。4.每(mei)(mei)(mei)孔(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)辣(la)(la)(la)根過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)(su)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)（同生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。5.溫育(yu)(yu)60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)(hou)，棄(qi)(qi)去(qu)孔(kong)內(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，甩(shuai)干(gan)，洗(xi)(xi)板(ban)(ban)5次(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)浸(jin)泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)，甩(shuai)干(gan)。6.依(yi)(yi)序(xu)每(mei)(mei)(mei)孔(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)(ye)90μl，37℃避光(guang)顯(xian)色(se)(se)(se)（30分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)，此(ci)(ci)時(shi)(shi)(shi)(shi)肉眼可(ke)(ke)(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)(qian)(qian)3-4孔(kong)有(you)(you)明顯(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)藍色(se)(se)(se)，后(hou)(hou)(hou)3-4孔(kong)梯度(du)(du)(du)不(bu)(bu)(bu)明顯(xian)，即(ji)(ji)可(ke)(ke)(ke)終(zhong)(zhong)止）。7.依(yi)(yi)序(xu)每(mei)(mei)(mei)孔(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)止溶(rong)液(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)止反(fan)應(ying)(ying)(ying)（此(ci)(ci)時(shi)(shi)(shi)(shi)藍色(se)(se)(se)立轉(zhuan)黃(huang)(huang)色(se)(se)(se)）。終(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)順(shun)序(xu)應(ying)(ying)(ying)盡量(liang)(liang)(liang)與底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)順(shun)序(xu)相(xiang)(xiang)(xiang)同。為(wei)(wei)了保(bao)(bao)證實(shi)驗(yan)結(jie)(jie)果的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)，底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)應(ying)(ying)(ying)時(shi)(shi)(shi)(shi)間到(dao)后(hou)(hou)(hou)應(ying)(ying)(ying)盡快加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)終(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)聯儀在(zai)(zai)450nm波(bo)長依(yi)(yi)序(xu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)各孔(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)密度(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)）。 在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)內(nei)(nei)(nei)(nei)(nei)進(jin)行檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。牛(niu)(niu)血(xue)(xue)(xue)(xue)管(guan)內(nei)(nei)(nei)(nei)(nei)皮(pi)(pi)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)粘(zhan)(zhan)附(fu)(fu)(fu)分(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)(zi)(zi)1(VCAM-1/CD106)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)注意事項(xiang)1.用(yong)(yong)(yong)戶在(zai)(zai)初次(ci)(ci)使(shi)(shi)用(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)時(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)將(jiang)(jiang)(jiang)各種試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)管(guan)離(li)(li)心(xin)數(shu)(shu)(shu)(shu)(shu)分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，以(yi)(yi)(yi)便試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)集中(zhong)到(dao)管(guan)底(di)(di)。2.每(mei)(mei)(mei)次(ci)(ci)實(shi)驗(yan)留一孔(kong)作(zuo)為(wei)(wei)空白(bai)調(diao)(diao)零孔(kong)，該(gai)孔(kong)不(bu)(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)任何試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，只(zhi)是**加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)(ye)及(ji)(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)時(shi)(shi)(shi)(shi)先(xian)用(yong)(yong)(yong)此(ci)(ci)孔(kong)調(diao)(diao)OD值(zhi)(zhi)(zhi)(zhi)至(zhi)零。 3.為(wei)(wei)防止樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)(shi)(shi)驗(yan)時(shi)(shi)(shi)(shi)將(jiang)(jiang)(jiang)反(fan)應(ying)(ying)(ying)板(ban)(ban)放(fang)(fang)于鋪有(you)(you)濕布(bu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)(he)(he)內(nei)(nei)(nei)(nei)(nei)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)(shang)蓋或(huo)(huo)(huo)(huo)覆膜。 4.未使(shi)(shi)用(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)或(huo)(huo)(huo)(huo)者試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，請于2-8℃保(bao)(bao)存(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)、辣(la)(la)(la)根過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)(su)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)請依(yi)(yi)據(ju)所(suo)(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)配(pei)(pei)置(zhi)(zhi)使(shi)(shi)用(yong)(yong)(yong)。請勿重復使(shi)(shi)用(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)(kang)體(ti)(ti)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)、辣(la)(la)(la)根過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)素(su)(su)(su)(su)(su)(su)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)。 5.建議檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)均設雙孔(kong)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定，以(yi)(yi)(yi)保(bao)(bao)證檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)果的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)。 牛(niu)(niu)血(xue)(xue)(xue)(xue)管(guan)內(nei)(nei)(nei)(nei)(nei)皮(pi)(pi)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)粘(zhan)(zhan)附(fu)(fu)(fu)分(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)(zi)(zi)1(VCAM-1/CD106)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)洗(xi)(xi)板(ban)(ban)方(fang)(fang)(fang)法手工(gong)(gong)洗(xi)(xi)板(ban)(ban)方(fang)(fang)(fang)法：吸(xi)(xi)去(qu)（不(bu)(bu)(bu)可(ke)(ke)(ke)觸(chu)及(ji)(ji)板(ban)(ban)壁(bi)）或(huo)(huo)(huo)(huo)甩(shuai)掉酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)內(nei)(nei)(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)；在(zai)(zai)實(shi)驗(yan)臺上(shang)(shang)(shang)(shang)鋪墊幾(ji)層(ceng)吸(xi)(xi)水紙(zhi)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)朝下(xia)(xia)用(yong)(yong)(yong)力拍幾(ji)次(ci)(ci)；將(jiang)(jiang)(jiang)推(tui)薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)(di)緩沖液(ye)(ye)(ye)(ye)(ye)至(zhi)少(shao)(shao)0.3ml注入(ru)(ru)(ru)孔(kong)內(nei)(nei)(nei)(nei)(nei)，浸(jin)泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。根據(ju)需(xu)要(yao)，重復此(ci)(ci)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)數(shu)(shu)(shu)(shu)(shu)次(ci)(ci)。 自(zi)(zi)動洗(xi)(xi)板(ban)(ban)：如(ru)(ru)(ru)果有(you)(you)自(zi)(zi)動洗(xi)(xi)板(ban)(ban)機，應(ying)(ying)(ying)在(zai)(zai)熟(shu)練使(shi)(shi)用(yong)(yong)(yong)后(hou)(hou)(hou)再(zai)用(yong)(yong)(yong)到(dao)正式(shi)(shi)(shi)實(shi)驗(yan)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)中(zhong)。 計(ji)算(suan)(suan) 以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)橫(heng)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)(shu)(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)(zhi)為(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普(pu)通坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)半對數(shu)(shu)(shu)(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)(shang)(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)，根據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)查出(chu)相(xiang)(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)；再(zai)乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)與OD值(zhi)(zhi)(zhi)(zhi)計(ji)算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)回(hui)歸方(fang)(fang)(fang)程(cheng)(cheng)式(shi)(shi)(shi)，將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)代入(ru)(ru)(ru)方(fang)(fang)(fang)程(cheng)(cheng)式(shi)(shi)(shi)，計(ji)算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)，再(zai)乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)(shu)，即(ji)(ji)為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際(ji)濃(nong)(nong)(nong)(nong)度(du)(du)(du)。 注意事項(xiang)1.當混(hun)(hun)合蛋白(bai)溶(rong)液(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)應(ying)(ying)(ying)盡量(liang)(liang)(liang)輕(qing)緩，避免起泡(pao)。2.洗(xi)(xi)滌(di)(di)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)非(fei)常重要(yao)，不(bu)(bu)(bu)充分(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)(di)易(yi)造(zao)成(cheng)假陽性(xing)(xing)。3.一次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)間**控制(zhi)(zhi)(zhi)在(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)，如(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)數(shu)(shu)(shu)(shu)(shu)量(liang)(liang)(liang)多，推(tui)薦使(shi)(shi)用(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)。4.請每(mei)(mei)(mei)次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)(shi)做(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)，做(zuo)(zuo)(zuo)復孔(kong)。5.如(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)待測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含(han)量(liang)(liang)(liang)過(guo)(guo)(guo)(guo)(guo)高，請先(xian)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)再(zai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定，計(ji)算(suan)(suan)時(shi)(shi)(shi)(shi)請乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)(shu)。6.在(zai)(zai)配(pei)(pei)制(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)液(ye)(ye)(ye)(ye)(ye)工(gong)(gong)作(zuo)液(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)，請以(yi)(yi)(yi)相(xiang)(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)配(pei)(pei)制(zhi)(zhi)(zhi)，不(bu)(bu)(bu)能混(hun)(hun)淆。7.底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請避光(guang)保(bao)(bao)存(cun)(cun)(cun)。 8.不(bu)(bu)(bu)要(yao)用(yong)(yong)(yong)其它生(sheng)(sheng)(sheng)產(chan)廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)替(ti)換(huan)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)。 注意事項(xiang)：收(shou)集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)前(qian)(qian)(qian)(qian)(qian)必須清(qing)(qing)楚(chu)要(yao)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)份是否(fou)足夠穩定。對收(shou)集后(hou)(hou)(hou)當天進(jin)行檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)，儲存(cun)(cun)(cun)在(zai)(zai)4 ℃備用(yong)(yong)(yong)，如(ru)(ru)(ru)有(you)(you)特殊原因需(xu)要(yao)周期(qi)收(shou)集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)， 將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)及(ji)(ji)時(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)(fen)裝后(hou)(hou)(hou)放(fang)(fang)在(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)-70 ℃條件下(xia)(xia)保(bao)(bao)存(cun)(cun)(cun)。避免反(fan)復凍(dong)(dong)融(rong)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)(ke)保(bao)(bao)存(cun)(cun)(cun)48 小(xiao)時(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)(ke)保(bao)(bao)存(cun)(cun)(cun)1 個月(yue)。-70度(du)(du)(du)可(ke)(ke)(ke)保(bao)(bao)存(cun)(cun)(cun)6 個月(yue)。部(bu)分(fen)(fen)(fen)(fen)(fen)(fen)激素(su)(su)(su)(su)(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)添加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)抑肽酶(mei)(mei)(mei)(mei)。計(ji)算(suan)(suan)：以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)橫(heng)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)為(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)(shang)(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)，根據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)查出(chu)相(xiang)(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)；再(zai)乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)與OD值(zhi)(zhi)(zhi)(zhi)計(ji)算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)回(hui)歸方(fang)(fang)(fang)程(cheng)(cheng)式(shi)(shi)(shi)，將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)代入(ru)(ru)(ru)方(fang)(fang)(fang)程(cheng)(cheng)式(shi)(shi)(shi)，計(ji)算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)，再(zai)乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)(shu)，即(ji)(ji)為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際(ji)濃(nong)(nong)(nong)(nong)度(du)(du)(du)。 牛(niu)(niu)血(xue)(xue)(xue)(xue)管(guan)內(nei)(nei)(nei)(nei)(nei)皮(pi)(pi)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)粘(zhan)(zhan)附(fu)(fu)(fu)分(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)(zi)(zi)1(VCAM-1/CD106)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)性(xing)(xing)能1.樣(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)線(xian)性(xing)(xing)回(hui)歸與預期(qi)濃(nong)(nong)(nong)(nong)度(du)(du)(du)相(xiang)(xiang)(xiang)關系(xi)數(shu)(shu)(shu)(shu)(shu)R值(zhi)(zhi)(zhi)(zhi)為(wei)(wei)0.92以(yi)(yi)(yi)上(shang)(shang)(shang)(shang)。2.批內(nei)(nei)(nei)(nei)(nei)與批見應(ying)(ying)(ying)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)小(xiao)于9%和(he)15% 牛(niu)(niu)血(xue)(xue)(xue)(xue)管(guan)內(nei)(nei)(nei)(nei)(nei)皮(pi)(pi)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)粘(zhan)(zhan)附(fu)(fu)(fu)分(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)(zi)(zi)1(VCAM-1/CD106)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)
牛葡萄(tao)(tao)(tao)糖(tang)(tang)調(diao)節(jie)(jie)蛋白(bai)78(GRP78) ELISA 試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)中(zhong)文(wen)名稱牛葡萄(tao)(tao)(tao)糖(tang)(tang)調(diao)節(jie)(jie)蛋白(bai)78(GRP78)ELISA 試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)規格96T/48T樣(yang)(yang)(yang)(yang)本(ben)(ben)類(lei)型血(xue)清、血(xue)漿、細胞上(shang)清液(ye)(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)(ye)、體液(ye)(ye)(ye)(ye)(ye)、灌洗(xi)(xi)(xi)液(ye)(ye)(ye)(ye)(ye)等使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)范(fan)圍科研實(shi)(shi)(shi)驗(yan)(yan)，不(bu)(bu)得用(yong)(yong)(yong)(yong)(yong)于臨床診斷反(fan)應(ying)(ying)(ying)時(shi)(shi)(shi)間1-5h試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)保(bao)(bao)(bao)存(cun)(cun)2-8℃有(you)(you)效期(qi)6個(ge)月貨期(qi)3-5天(tian)【操(cao)作(zuo)步驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)與加(jia)(jia)樣(yang)(yang)(yang)(yang)：在酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)上(shang)設標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)，在第(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)分(fen)別(bie)(bie)(bie)加(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，然(ran)后(hou)(hou)(hou)(hou)(hou)在第(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)加(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻；然(ran)后(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)各(ge)取(qu)(qu)(qu)100μl分(fen)別(bie)(bie)(bie)加(jia)(jia)到(dao)(dao)(dao)第(di)(di)(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)第(di)(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)在第(di)(di)(di)(di)(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)分(fen)別(bie)(bie)(bie)加(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻；然(ran)后(hou)(hou)(hou)(hou)(hou)在第(di)(di)(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)第(di)(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)先(xian)各(ge)取(qu)(qu)(qu)50μl棄(qi)掉(diao)(diao)，再(zai)各(ge)取(qu)(qu)(qu)50μl分(fen)別(bie)(bie)(bie)加(jia)(jia)到(dao)(dao)(dao)第(di)(di)(di)(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，再(zai)在第(di)(di)(di)(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)分(fen)別(bie)(bie)(bie)加(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50ul，混(hun)(hun)勻；混(hun)(hun)勻后(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)各(ge)取(qu)(qu)(qu)50μl分(fen)別(bie)(bie)(bie)加(jia)(jia)到(dao)(dao)(dao)第(di)(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，再(zai)在第(di)(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)分(fen)別(bie)(bie)(bie)加(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻后(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)分(fen)別(bie)(bie)(bie)取(qu)(qu)(qu)50μl加(jia)(jia)到(dao)(dao)(dao)第(di)(di)(di)(di)(di)(di)(di)(di)九、第(di)(di)(di)(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，再(zai)在第(di)(di)(di)(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)分(fen)別(bie)(bie)(bie)加(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻后(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)各(ge)取(qu)(qu)(qu)50μl棄(qi)掉(diao)(diao)。（稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)樣(yang)(yang)(yang)(yang)量(liang)都為50μl，濃(nong)(nong)度(du)分(fen)別(bie)(bie)(bie)為3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)樣(yang)(yang)(yang)(yang)：分(fen)別(bie)(bie)(bie)設空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)白(bai)對(dui)照(zhao)孔(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)及(ji)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，其(qi)余各(ge)步操(cao)作(zuo)相(xiang)同）、待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)。在酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)上(shang)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)先(xian)加(jia)(jia)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)40μl，然(ran)后(hou)(hou)(hou)(hou)(hou)再(zai)加(jia)(jia)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)＊終(zhong)(zhong)(zhong)稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)度(du)為5倍(bei)(bei)(bei)(bei)）。加(jia)(jia)樣(yang)(yang)(yang)(yang)將樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)于酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)底(di)部，盡(jin)量(liang)不(bu)(bu)觸及(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)壁，輕輕晃(huang)動混(hun)(hun)勻。3. 溫育：用(yong)(yong)(yong)(yong)(yong)封(feng)(feng)(feng)板(ban)(ban)膜(mo)封(feng)(feng)(feng)板(ban)(ban)后(hou)(hou)(hou)(hou)(hou)置37℃溫育30分(fen)鐘(zhong)。4. 配液(ye)(ye)(ye)(ye)(ye)：將30（48T的(de)(de)20倍(bei)(bei)(bei)(bei)）倍(bei)(bei)(bei)(bei)濃(nong)(nong)縮洗(xi)(xi)(xi)滌液(ye)(ye)(ye)(ye)(ye)用(yong)(yong)(yong)(yong)(yong)蒸餾水30（48T的(de)(de)20倍(bei)(bei)(bei)(bei)）倍(bei)(bei)(bei)(bei)稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)備用(yong)(yong)(yong)(yong)(yong)。5. 洗(xi)(xi)(xi)滌：小(xiao)心揭(jie)掉(diao)(diao)封(feng)(feng)(feng)板(ban)(ban)膜(mo)，棄(qi)去液(ye)(ye)(ye)(ye)(ye)體，甩干(gan)，每孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)滿洗(xi)(xi)(xi)滌液(ye)(ye)(ye)(ye)(ye)，靜置30秒后(hou)(hou)(hou)(hou)(hou)棄(qi)去，如此重復5次(ci)(ci)，拍干(gan)。6. 加(jia)(jia)酶(mei)(mei)(mei)(mei)：每孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)入酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)50μl，空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)除(chu)外。7. 溫育：操(cao)作(zuo)同3。8. 洗(xi)(xi)(xi)滌：操(cao)作(zuo)同5。9. 顯(xian)色(se)：每孔(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)入顯(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)A50μl，再(zai)加(jia)(jia)入顯(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)B50μl，輕輕震(zhen)蕩混(hun)(hun)勻，37℃避光顯(xian)色(se)15分(fen)鐘(zhong). 10. 終(zhong)(zhong)(zhong)止(zhi)：每孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)終(zhong)(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)(zhong)止(zhi)反(fan)應(ying)(ying)(ying)（此時(shi)(shi)(shi)藍色(se)立轉黃色(se)）。11. 測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)：以空(kong)白(bai)空(kong)調(diao)零，450nm波(bo)長依序(xu)測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)吸光度(du)（OD值(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)應(ying)(ying)(ying)在加(jia)(jia)終(zhong)(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)(hou)15分(fen)鐘(zhong)以內(nei)進行。牛葡萄(tao)(tao)(tao)糖(tang)(tang)調(diao)節(jie)(jie)蛋白(bai)78(GRP78)ELISA 試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)【注意事(shi)項(xiang)】1． 試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)從(cong)冷藏(zang)環境(jing)中(zhong)取(qu)(qu)(qu)出(chu)(chu)應(ying)(ying)(ying)在室溫平衡15-30分(fen)鐘(zhong)后(hou)(hou)(hou)(hou)(hou)方可(ke)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)開封(feng)(feng)(feng)后(hou)(hou)(hou)(hou)(hou)如未(wei)用(yong)(yong)(yong)(yong)(yong)完(wan)，板(ban)(ban)條(tiao)應(ying)(ying)(ying)裝入密(mi)(mi)(mi)封(feng)(feng)(feng)袋(dai)(dai)中(zhong)保(bao)(bao)(bao)存(cun)(cun)。2． 濃(nong)(nong)洗(xi)(xi)(xi)滌液(ye)(ye)(ye)(ye)(ye)可(ke)能(neng)會有(you)(you)結晶析(xi)出(chu)(chu)，稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)可(ke)在水浴中(zhong)加(jia)(jia)溫助溶(rong)，洗(xi)(xi)(xi)滌時(shi)(shi)(shi)不(bu)(bu)影響結果(guo)。3． 各(ge)步加(jia)(jia)樣(yang)(yang)(yang)(yang)均(jun)應(ying)(ying)(ying)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)加(jia)(jia)樣(yang)(yang)(yang)(yang)器(qi)，并經常(chang)校對(dui)其(qi)準(zhun)(zhun)(zhun)確性(xing)，以避免試(shi)(shi)驗(yan)(yan)誤差(cha)。一(yi)(yi)次(ci)(ci)加(jia)(jia)樣(yang)(yang)(yang)(yang)時(shi)(shi)(shi)間＊好(hao)控制(zhi)(zhi)在5分(fen)鐘(zhong)內(nei)，如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)數(shu)量(liang)多，推(tui)薦使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)樣(yang)(yang)(yang)(yang)。4． 請(qing)每次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)的(de)(de)同時(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)線，＊好(hao)做(zuo)復孔(kong)(kong)(kong)(kong)(kong)(kong)。如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)中(zhong)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)質(zhi)含(han)量(liang)過高(gao)(gao)（樣(yang)(yang)(yang)(yang)本(ben)(ben)OD值(zhi)(zhi)(zhi)大于標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)OD值(zhi)(zhi)(zhi)），請(qing)先(xian)用(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)一(yi)(yi)定(ding)(ding)(ding)倍(bei)(bei)(bei)(bei)數(shu)（n倍(bei)(bei)(bei)(bei)）后(hou)(hou)(hou)(hou)(hou)再(zai)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，計算(suan)(suan)時(shi)(shi)(shi)請(qing)＊后(hou)(hou)(hou)(hou)(hou)乘(cheng)以總(zong)稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)(bei)數(shu)（×n×5）。5． 封(feng)(feng)(feng)板(ban)(ban)膜(mo)只限(xian)一(yi)(yi)次(ci)(ci)性(xing)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)，以避免交叉污染(ran)。6． 底(di)物(wu)(wu)(wu)(wu)(wu)請(qing)避光保(bao)(bao)(bao)存(cun)(cun)。7． 嚴格按(an)照(zhao)說明(ming)(ming)(ming)書(shu)的(de)(de)操(cao)作(zuo)進行，試(shi)(shi)驗(yan)(yan)結果(guo)判定(ding)(ding)(ding)必(bi)須以酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀(du)數(shu)為準(zhun)(zhun)(zhun).8． 所有(you)(you)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)，洗(xi)(xi)(xi)滌液(ye)(ye)(ye)(ye)(ye)和(he)(he)各(ge)種(zhong)廢(fei)棄(qi)物(wu)(wu)(wu)(wu)(wu)都應(ying)(ying)(ying)按(an)傳(chuan)染(ran)物(wu)(wu)(wu)(wu)(wu)處理。9． 本(ben)(ben)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)不(bu)(bu)同批(pi)號組(zu)(zu)分(fen)不(bu)(bu)得混(hun)(hun)用(yong)(yong)(yong)(yong)(yong)。10. 如與英文(wen)說明(ming)(ming)(ming)書(shu)有(you)(you)異(yi)，以英文(wen)說明(ming)(ming)(ming)書(shu)為準(zhun)(zhun)(zhun)。【計算(suan)(suan)】 以標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)的(de)(de)濃(nong)(nong)度(du)為橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)， 在坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)繪出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)線，根據(ju)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)OD 值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)線查(cha)出(chu)(chu)相(xiang)應(ying)(ying)(ying)的(de)(de)濃(nong)(nong)度(du)；再(zai)乘(cheng)以稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi) 倍(bei)(bei)(bei)(bei)數(shu)；或(huo)用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)的(de)(de)濃(nong)(nong)度(du)與OD值(zhi)(zhi)(zhi)計算(suan)(suan)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)曲(qu)線的(de)(de)直線回歸方程式，將樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)OD值(zhi)(zhi)(zhi) 代入方程式，計算(suan)(suan)出(chu)(chu)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)，再(zai)乘(cheng)以稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi) 倍(bei)(bei)(bei)(bei)數(shu)，即(ji)為樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)實(shi)(shi)(shi)際(ji)濃(nong)(nong)度(du)。 【試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)組(zu)(zu)成(cheng)】試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)組(zu)(zu)成(cheng)48孔(kong)(kong)(kong)(kong)(kong)(kong)配置96孔(kong)(kong)(kong)(kong)(kong)(kong)配置保(bao)(bao)(bao)存(cun)(cun)說明(ming)(ming)(ming)書(shu)1份(fen)1份(fen)密(mi)(mi)(mi)封(feng)(feng)(feng)袋(dai)(dai)1個(ge)1個(ge)封(feng)(feng)(feng)板(ban)(ban)膜(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)2片（96）2-8℃保(bao)(bao)(bao)存(cun)(cun)微孔(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)2-8℃保(bao)(bao)(bao)存(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管(guan)0.3mL*6管(guan)2-8℃保(bao)(bao)(bao)存(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)顯(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)顯(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)終(zhong)(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)注意：使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前請(qing)檢查(cha)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)中(zhong)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和(he)(he)數(shu)量(liang)與表格是(shi)否一(yi)(yi)致。【精密(mi)(mi)(mi)度(du)】精密(mi)(mi)(mi)度(du)用(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)的(de)(de)變異(yi)系數(shu)CV 表示。CV（%） = SD/mean×100批(pi)內(nei)差(cha)：取(qu)(qu)(qu)同批(pi)次(ci)(ci)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)對(dui)低(di)、中(zhong)、高(gao)(gao)值(zhi)(zhi)(zhi)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)本(ben)(ben)進行定(ding)(ding)(ding)量(liang)檢測(ce)(ce)(ce)(ce)(ce)(ce)，每份(fen)樣(yang)(yang)(yang)(yang)本(ben)(ben)連續測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)20 次(ci)(ci)，分(fen)別(bie)(bie)(bie)計算(suan)(suan)不(bu)(bu)同濃(nong)(nong)度(du)樣(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)平均(jun)值(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)。批(pi)內(nei)差(cha)： CV<9%批(pi)間差(cha)：選(xuan)取(qu)(qu)(qu)3 個(ge)不(bu)(bu)同批(pi)次(ci)(ci)的(de)(de)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)分(fen)別(bie)(bie)(bie)對(dui)低(di)、中(zhong)、高(gao)(gao)值(zhi)(zhi)(zhi)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)本(ben)(ben)進行定(ding)(ding)(ding)量(liang)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，每個(ge)樣(yang)(yang)(yang)(yang)本(ben)(ben)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)同一(yi)(yi)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)重復測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)10 次(ci)(ci)，分(fen)別(bie)(bie)(bie)計算(suan)(suan)不(bu)(bu)同濃(nong)(nong)度(du)樣(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)平均(jun)值(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)。批(pi)間差(cha)： CV<11% 【特異(yi)性(xing)】本(ben)(ben)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)識(shi)別(bie)(bie)(bie)天(tian)然(ran)和(he)(he)重組(zu)(zu)微生物(wu)(wu)(wu)(wu)(wu)硝酸鹽還原酶(mei)(mei)(mei)(mei)（Nar），經檢測(ce)(ce)(ce)(ce)(ce)(ce)與其(qi)它相(xiang)似物(wu)(wu)(wu)(wu)(wu)質(zhi)無明(ming)(ming)(ming)顯(xian)交叉反(fan)應(ying)(ying)(ying)。由于受到(dao)(dao)(dao)技(ji)術及(ji)樣(yang)(yang)(yang)(yang)本(ben)(ben)來源的(de)(de)限(xian)制(zhi)(zhi)，不(bu)(bu)可(ke)能(neng)完(wan)成(cheng)所有(you)(you)相(xiang)關或(huo)相(xiang)似物(wu)(wu)(wu)(wu)(wu)質(zhi)交叉反(fan)應(ying)(ying)(ying)檢測(ce)(ce)(ce)(ce)(ce)(ce)，因此本(ben)(ben)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)有(you)(you)可(ke)能(neng)與未(wei)經檢測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)其(qi)它物(wu)(wu)(wu)(wu)(wu)質(zhi)有(you)(you)交叉反(fan)應(ying)(ying)(ying)。 【穩定(ding)(ding)(ding)性(xing)】試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)在有(you)(you)效期(qi)內(nei)務必(bi)按(an)推(tui)薦溫度(du)保(bao)(bao)(bao)存(cun)(cun)，活性(xing)降低(di)率小(xiao)于5%。為減(jian)小(xiao)外部因素對(dui)試(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)破壞(huai)前后(hou)(hou)(hou)(hou)(hou)檢測(ce)(ce)(ce)(ce)(ce)(ce)值(zhi)(zhi)(zhi)的(de)(de)影響，實(shi)(shi)(shi)驗(yan)(yan)室的(de)(de)環境(jing)條(tiao)件需盡(jin)量(liang)保(bao)(bao)(bao)持一(yi)(yi)致，尤其(qi)是(shi)實(shi)(shi)(shi)驗(yan)(yan)室內(nei)溫度(du)、濕度(du)及(ji)溫育條(tiao)件。其(qi)次(ci)(ci)由同一(yi)(yi)實(shi)(shi)(shi)驗(yan)(yan)員來進行操(cao)作(zuo)可(ke)減(jian)少人為誤差(cha)
牛(niu)角(jiao)蛋(dan)白(bai)(bai)1(KRT1) ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)文(wen)(wen)名稱(cheng)牛(niu)角(jiao)蛋(dan)白(bai)(bai)1(KRT1) ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)規格(ge)96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)類型血清、血漿、細胞上清液(ye)(ye)、尿(niao)液(ye)(ye)、體液(ye)(ye)、灌洗(xi)(xi)液(ye)(ye)等使用(yong)范圍(wei)科(ke)研實(shi)驗(yan)，不(bu)得(de)用(yong)于(yu)臨床診斷(duan)反應(ying)時間1-5h試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)2-8℃有(you)效(xiao)(xiao)期6個(ge)(ge)月貨(huo)期3-5天(tian)【操(cao)作(zuo)(zuo)(zuo)步驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)與(yu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)(zai)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)上設(she)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)一、第(di)(di)(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)100μl，然(ran)后(hou)(hou)(hou)(hou)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)一、第(di)(di)(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)50μl，混(hun)勻(yun)；然(ran)后(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)(di)一孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)取100μl分(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)第(di)(di)(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)50μl，混(hun)勻(yun)；然(ran)后(hou)(hou)(hou)(hou)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)第(di)(di)(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)先(xian)各(ge)取50μl棄(qi)掉(diao)，再(zai)(zai)各(ge)取50μl分(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)50ul，混(hun)勻(yun)；混(hun)勻(yun)后(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)取50μl分(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)50μl，混(hun)勻(yun)后(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)(di)七(qi)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)(bie)取50μl加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)(di)(di)九、第(di)(di)(di)(di)(di)(di)(di)(di)(di)十(shi)(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)(di)(di)(di)(di)十(shi)(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)50μl，混(hun)勻(yun)后(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)(di)九第(di)(di)(di)(di)(di)(di)(di)(di)(di)十(shi)(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)取50μl棄(qi)掉(diao)。（稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)后(hou)(hou)(hou)(hou)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)都為(wei)50μl，濃(nong)度(du)(du)(du)(du)分(fen)(fen)別(bie)(bie)(bie)(bie)為(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)別(bie)(bie)(bie)(bie)設(she)空(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)白(bai)(bai)對照(zhao)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)及酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)，其(qi)余(yu)各(ge)步操(cao)作(zuo)(zuo)(zuo)相同(tong)）、待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)(zai)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)上待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)40μl，然(ran)后(hou)(hou)(hou)(hou)再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)待測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)＊終稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)度(du)(du)(du)(du)為(wei)5倍(bei)）。加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部，盡量(liang)(liang)不(bu)觸及孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕輕晃動(dong)混(hun)勻(yun)。3. 溫育：用(yong)封板(ban)(ban)膜(mo)封板(ban)(ban)后(hou)(hou)(hou)(hou)置(zhi)37℃溫育30分(fen)(fen)鐘(zhong)。4. 配液(ye)(ye)：將(jiang)(jiang)30（48T的(de)(de)(de)(de)(de)20倍(bei)）倍(bei)濃(nong)縮洗(xi)(xi)滌(di)(di)(di)(di)液(ye)(ye)用(yong)蒸餾(liu)水30（48T的(de)(de)(de)(de)(de)20倍(bei)）倍(bei)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)后(hou)(hou)(hou)(hou)備用(yong)。5. 洗(xi)(xi)滌(di)(di)(di)(di)：小心揭掉(diao)封板(ban)(ban)膜(mo)，棄(qi)去液(ye)(ye)體，甩干，每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)滿洗(xi)(xi)滌(di)(di)(di)(di)液(ye)(ye)，靜置(zhi)30秒后(hou)(hou)(hou)(hou)棄(qi)去，如(ru)(ru)(ru)(ru)此重復(fu)5次(ci)(ci)，拍(pai)干。6. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)50μl，空(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外(wai)(wai)。7. 溫育：操(cao)作(zuo)(zuo)(zuo)同(tong)3。8. 洗(xi)(xi)滌(di)(di)(di)(di)：操(cao)作(zuo)(zuo)(zuo)同(tong)5。9. 顯(xian)(xian)色(se)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入顯(xian)(xian)色(se)劑(ji)A50μl，再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入顯(xian)(xian)色(se)劑(ji)B50μl，輕輕震蕩混(hun)勻(yun)，37℃避(bi)(bi)光(guang)顯(xian)(xian)色(se)15分(fen)(fen)鐘(zhong). 10. 終止(zhi)(zhi)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終止(zhi)(zhi)液(ye)(ye)50μl，終止(zhi)(zhi)反應(ying)（此時藍色(se)立(li)轉黃(huang)色(se)）。11. 測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)：以(yi)(yi)空(kong)白(bai)(bai)空(kong)調(diao)零，450nm波(bo)長依序測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)吸光(guang)度(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)應(ying)在(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終止(zhi)(zhi)液(ye)(ye)后(hou)(hou)(hou)(hou)15分(fen)(fen)鐘(zhong)以(yi)(yi)內(nei)(nei)進(jin)行(xing)(xing)。牛(niu)角(jiao)蛋(dan)白(bai)(bai)1(KRT1) ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)【注意(yi)(yi)事項(xiang)】1． 試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)從(cong)冷藏(zang)環境中(zhong)(zhong)(zhong)(zhong)(zhong)取出(chu)(chu)(chu)應(ying)在(zai)(zai)(zai)室溫平衡15-30分(fen)(fen)鐘(zhong)后(hou)(hou)(hou)(hou)方可(ke)(ke)使用(yong)，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)開封后(hou)(hou)(hou)(hou)如(ru)(ru)(ru)(ru)未(wei)用(yong)完，板(ban)(ban)條應(ying)裝入密(mi)封袋中(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)。2． 濃(nong)洗(xi)(xi)滌(di)(di)(di)(di)液(ye)(ye)可(ke)(ke)能(neng)(neng)(neng)會(hui)有(you)結晶析出(chu)(chu)(chu)，稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)時可(ke)(ke)在(zai)(zai)(zai)水浴中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)溫助溶(rong)，洗(xi)(xi)滌(di)(di)(di)(di)時不(bu)影響結果。3． 各(ge)步加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)均(jun)應(ying)使用(yong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)器，并經常校對其(qi)準(zhun)(zhun)(zhun)確性，以(yi)(yi)避(bi)(bi)免試(shi)(shi)(shi)(shi)(shi)驗(yan)誤差(cha)。一次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時間＊好(hao)控制在(zai)(zai)(zai)5分(fen)(fen)鐘(zhong)內(nei)(nei)，如(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數量(liang)(liang)多，推(tui)薦使用(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4． 請每(mei)次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)同(tong)時做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲線(xian)(xian)，＊好(hao)做復(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)待測(ce)(ce)(ce)(ce)(ce)物(wu)質(zhi)含量(liang)(liang)過高(gao)(gao)(gao)（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)大于(yu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)(di)(di)(di)一孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)），請先(xian)用(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)一定(ding)(ding)(ding)(ding)(ding)倍(bei)數（n倍(bei)）后(hou)(hou)(hou)(hou)再(zai)(zai)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)，計(ji)算(suan)時請＊后(hou)(hou)(hou)(hou)乘(cheng)以(yi)(yi)總稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)倍(bei)數（×n×5）。5． 封板(ban)(ban)膜(mo)只限一次(ci)(ci)性使用(yong)，以(yi)(yi)避(bi)(bi)免交(jiao)叉污染(ran)。6． 底物(wu)請避(bi)(bi)光(guang)保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)。7． 嚴(yan)格(ge)按照(zhao)說(shuo)明(ming)書的(de)(de)(de)(de)(de)操(cao)作(zuo)(zuo)(zuo)進(jin)行(xing)(xing)，試(shi)(shi)(shi)(shi)(shi)驗(yan)結果判定(ding)(ding)(ding)(ding)(ding)必須以(yi)(yi)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀數為(wei)準(zhun)(zhun)(zhun).8． 所有(you)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)，洗(xi)(xi)滌(di)(di)(di)(di)液(ye)(ye)和(he)(he)各(ge)種廢棄(qi)物(wu)都應(ying)按傳染(ran)物(wu)處理。9． 本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)不(bu)同(tong)批(pi)(pi)號組(zu)分(fen)(fen)不(bu)得(de)混(hun)用(yong)。10. 如(ru)(ru)(ru)(ru)與(yu)英(ying)文(wen)(wen)說(shuo)明(ming)書有(you)異，以(yi)(yi)英(ying)文(wen)(wen)說(shuo)明(ming)書為(wei)準(zhun)(zhun)(zhun)。【計(ji)算(suan)】 以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)的(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)(du)為(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)縱(zong)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上繪出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲線(xian)(xian)，根據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)OD 值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)由(you)(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲線(xian)(xian)查出(chu)(chu)(chu)相應(ying)的(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)(du)；再(zai)(zai)乘(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi) 倍(bei)數；或(huo)用(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)的(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)計(ji)算(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)曲線(xian)(xian)的(de)(de)(de)(de)(de)直線(xian)(xian)回歸方程式(shi)，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi) 代入方程式(shi)，計(ji)算(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)度(du)(du)(du)(du)，再(zai)(zai)乘(cheng)以(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi) 倍(bei)數，即為(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)實(shi)際濃(nong)度(du)(du)(du)(du)。 【試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)組(zu)成】試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)組(zu)成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)說(shuo)明(ming)書1份(fen)1份(fen)密(mi)封袋1個(ge)(ge)1個(ge)(ge)封板(ban)(ban)膜(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條2片（96）2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)顯(xian)(xian)色(se)劑(ji)A液(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)顯(xian)(xian)色(se)劑(ji)B液(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)終止(zhi)(zhi)液(ye)(ye)3ml×1瓶(ping)(ping)(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)注意(yi)(yi)：使用(yong)前(qian)請檢(jian)(jian)(jian)(jian)查試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)劑(ji)的(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和(he)(he)數量(liang)(liang)與(yu)表(biao)格(ge)是否一致。【精(jing)密(mi)度(du)(du)(du)(du)】精(jing)密(mi)度(du)(du)(du)(du)用(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)變異系數CV 表(biao)示(shi)。CV（%） = SD/mean×100批(pi)(pi)內(nei)(nei)差(cha)：取同(tong)批(pi)(pi)次(ci)(ci)試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)對低、中(zhong)(zhong)(zhong)(zhong)(zhong)、高(gao)(gao)(gao)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)進(jin)行(xing)(xing)定(ding)(ding)(ding)(ding)(ding)量(liang)(liang)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，每(mei)份(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)連續測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)20 次(ci)(ci)，分(fen)(fen)別(bie)(bie)(bie)(bie)計(ji)算(suan)不(bu)同(tong)濃(nong)度(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)平均(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)及SD 值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。批(pi)(pi)內(nei)(nei)差(cha)： CV<9%批(pi)(pi)間差(cha)：選取3 個(ge)(ge)不(bu)同(tong)批(pi)(pi)次(ci)(ci)的(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)分(fen)(fen)別(bie)(bie)(bie)(bie)對低、中(zhong)(zhong)(zhong)(zhong)(zhong)、高(gao)(gao)(gao)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)進(jin)行(xing)(xing)定(ding)(ding)(ding)(ding)(ding)量(liang)(liang)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)，每(mei)個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)使用(yong)同(tong)一試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)重復(fu)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)10 次(ci)(ci)，分(fen)(fen)別(bie)(bie)(bie)(bie)計(ji)算(suan)不(bu)同(tong)濃(nong)度(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)平均(jun)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)及SD 值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。批(pi)(pi)間差(cha)： CV<11% 【特(te)異性】本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)識別(bie)(bie)(bie)(bie)天(tian)然(ran)和(he)(he)重組(zu)微生物(wu)硝酸(suan)鹽還原酶(mei)(mei)（Nar），經檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)與(yu)其(qi)它相似物(wu)質(zhi)無明(ming)顯(xian)(xian)交(jiao)叉反應(ying)。由(you)(you)于(yu)受到(dao)技術及樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)來源的(de)(de)(de)(de)(de)限制，不(bu)可(ke)(ke)能(neng)(neng)(neng)完成所有(you)相關或(huo)相似物(wu)質(zhi)交(jiao)叉反應(ying)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，因此本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)有(you)可(ke)(ke)能(neng)(neng)(neng)與(yu)未(wei)經檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)其(qi)它物(wu)質(zhi)有(you)交(jiao)叉反應(ying)。 【穩定(ding)(ding)(ding)(ding)(ding)性】試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)在(zai)(zai)(zai)有(you)效(xiao)(xiao)期內(nei)(nei)務必按推(tui)薦溫度(du)(du)(du)(du)保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)，活(huo)性降低率小于(yu)5%。為(wei)減(jian)小外(wai)(wai)部因素對試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)破(po)壞(huai)前(qian)后(hou)(hou)(hou)(hou)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)值(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)影響，實(shi)驗(yan)室的(de)(de)(de)(de)(de)環境條件(jian)需盡量(liang)(liang)保(bao)(bao)(bao)持(chi)一致，尤其(qi)是實(shi)驗(yan)室內(nei)(nei)溫度(du)(du)(du)(du)、濕度(du)(du)(du)(du)及溫育條件(jian)。其(qi)次(ci)(ci)由(you)(you)同(tong)一實(shi)驗(yan)員來進(jin)行(xing)(xing)操(cao)作(zuo)(zuo)(zuo)可(ke)(ke)減(jian)少人為(wei)誤差(cha)。
牛(niu)(niu)白介素1β前(qian)體(ti)(pro-IL1β) ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)文(wen)名(ming)稱牛(niu)(niu)白介素1β前(qian)體(ti)(pro-IL1β) ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)規格96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)類型血清、血漿(jiang)、細胞上(shang)(shang)清液(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)、體(ti)液(ye)(ye)(ye)(ye)、灌洗液(ye)(ye)(ye)(ye)等使(shi)(shi)用(yong)(yong)范圍科研實(shi)驗(yan)，不(bu)(bu)得用(yong)(yong)于(yu)(yu)臨床診斷反應(ying)(ying)(ying)時(shi)(shi)(shi)(shi)間1-5h試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)2-8℃有(you)效期(qi)6個月貨期(qi)3-5天【操(cao)(cao)(cao)作(zuo)(zuo)步(bu)驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)與(yu)(yu)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被板(ban)(ban)上(shang)(shang)設標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，然(ran)后(hou)(hou)在(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)(yun)；然(ran)后(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各取100μl分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)在(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)三、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)(yun)；然(ran)后(hou)(hou)在(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)先(xian)(xian)各取50μl棄(qi)掉，再(zai)(zai)各取50μl分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)50ul，混(hun)勻(yun)(yun)；混(hun)勻(yun)(yun)后(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)五(wu)(wu)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各取50μl分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)(yun)后(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)八(ba)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)取50μl加(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)九(jiu)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)勻(yun)(yun)后(hou)(hou)從(cong)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)各取50μl棄(qi)掉。（稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)后(hou)(hou)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)(liang)(liang)都(dou)為(wei)50μl，濃度(du)(du)(du)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)為(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)設空(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)白對(dui)照(zhao)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)及(ji)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)，其(qi)余各步(bu)操(cao)(cao)(cao)作(zuo)(zuo)相同(tong)(tong)(tong)）、待測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被板(ban)(ban)上(shang)(shang)待測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)先(xian)(xian)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)40μl，然(ran)后(hou)(hou)再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)待測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)＊終(zhong)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)度(du)(du)(du)為(wei)5倍(bei)(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)于(yu)(yu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)底(di)部，盡(jin)量(liang)(liang)(liang)(liang)不(bu)(bu)觸及(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)(qing)輕(qing)(qing)晃(huang)動(dong)混(hun)勻(yun)(yun)。3. 溫(wen)(wen)(wen)(wen)育(yu)：用(yong)(yong)封(feng)(feng)(feng)(feng)板(ban)(ban)膜封(feng)(feng)(feng)(feng)板(ban)(ban)后(hou)(hou)置(zhi)37℃溫(wen)(wen)(wen)(wen)育(yu)30分(fen)(fen)(fen)(fen)鐘(zhong)。4. 配(pei)液(ye)(ye)(ye)(ye)：將(jiang)30（48T的(de)20倍(bei)(bei)(bei)）倍(bei)(bei)(bei)濃縮洗滌(di)液(ye)(ye)(ye)(ye)用(yong)(yong)蒸餾水(shui)30（48T的(de)20倍(bei)(bei)(bei)）倍(bei)(bei)(bei)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)后(hou)(hou)備(bei)用(yong)(yong)。5. 洗滌(di)：小心揭(jie)掉封(feng)(feng)(feng)(feng)板(ban)(ban)膜，棄(qi)去液(ye)(ye)(ye)(ye)體(ti)，甩干(gan)，每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)滿洗滌(di)液(ye)(ye)(ye)(ye)，靜置(zhi)30秒后(hou)(hou)棄(qi)去，如此(ci)(ci)重(zhong)復5次(ci)(ci)(ci)(ci)(ci)，拍(pai)干(gan)。6. 加(jia)(jia)(jia)(jia)(jia)酶(mei)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)入酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)50μl，空(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外。7. 溫(wen)(wen)(wen)(wen)育(yu)：操(cao)(cao)(cao)作(zuo)(zuo)同(tong)(tong)(tong)3。8. 洗滌(di)：操(cao)(cao)(cao)作(zuo)(zuo)同(tong)(tong)(tong)5。9. 顯(xian)色(se)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)(xian)加(jia)(jia)(jia)(jia)(jia)入顯(xian)色(se)劑(ji)(ji)(ji)A50μl，再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)入顯(xian)色(se)劑(ji)(ji)(ji)B50μl，輕(qing)(qing)輕(qing)(qing)震(zhen)蕩混(hun)勻(yun)(yun)，37℃避(bi)光(guang)顯(xian)色(se)15分(fen)(fen)(fen)(fen)鐘(zhong). 10. 終(zhong)止(zhi)(zhi)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)50μl，終(zhong)止(zhi)(zhi)反應(ying)(ying)(ying)（此(ci)(ci)時(shi)(shi)(shi)(shi)藍(lan)色(se)立轉黃色(se)）。11. 測(ce)(ce)定(ding)(ding)：以(yi)(yi)空(kong)白空(kong)調零，450nm波(bo)長依(yi)序測(ce)(ce)量(liang)(liang)(liang)(liang)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)吸光(guang)度(du)(du)(du)（OD值(zhi)）。 測(ce)(ce)定(ding)(ding)應(ying)(ying)(ying)在(zai)加(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)后(hou)(hou)15分(fen)(fen)(fen)(fen)鐘(zhong)以(yi)(yi)內(nei)進(jin)行(xing)。牛(niu)(niu)白介素1β前(qian)體(ti)(pro-IL1β) ELISA 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)【注意(yi)(yi)事項】1． 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)從(cong)冷藏環境(jing)(jing)中(zhong)(zhong)(zhong)(zhong)取出(chu)應(ying)(ying)(ying)在(zai)室溫(wen)(wen)(wen)(wen)平(ping)衡15-30分(fen)(fen)(fen)(fen)鐘(zhong)后(hou)(hou)方可使(shi)(shi)用(yong)(yong)，酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被板(ban)(ban)開封(feng)(feng)(feng)(feng)后(hou)(hou)如未(wei)用(yong)(yong)完，板(ban)(ban)條(tiao)應(ying)(ying)(ying)裝入密(mi)封(feng)(feng)(feng)(feng)袋中(zhong)(zhong)(zhong)(zhong)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)。2． 濃洗滌(di)液(ye)(ye)(ye)(ye)可能(neng)會有(you)結(jie)晶析(xi)出(chu)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)時(shi)(shi)(shi)(shi)可在(zai)水(shui)浴中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)溫(wen)(wen)(wen)(wen)助(zhu)溶，洗滌(di)時(shi)(shi)(shi)(shi)不(bu)(bu)影響(xiang)結(jie)果。3． 各步(bu)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)均(jun)(jun)應(ying)(ying)(ying)使(shi)(shi)用(yong)(yong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)器，并經常校對(dui)其(qi)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)，以(yi)(yi)避(bi)免試(shi)(shi)(shi)(shi)(shi)驗(yan)誤差(cha)(cha)。一(yi)(yi)(yi)(yi)次(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)間＊好(hao)控(kong)制(zhi)在(zai)5分(fen)(fen)(fen)(fen)鐘(zhong)內(nei)，如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)(shu)量(liang)(liang)(liang)(liang)多，推(tui)薦(jian)使(shi)(shi)用(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4． 請(qing)每(mei)(mei)次(ci)(ci)(ci)(ci)(ci)測(ce)(ce)定(ding)(ding)的(de)同(tong)(tong)(tong)時(shi)(shi)(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線，＊好(hao)做復孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)待測(ce)(ce)物(wu)(wu)(wu)質(zhi)含(han)量(liang)(liang)(liang)(liang)過(guo)高(gao)(gao)（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)OD值(zhi)大于(yu)(yu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)OD值(zhi)），請(qing)先(xian)(xian)用(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)一(yi)(yi)(yi)(yi)定(ding)(ding)倍(bei)(bei)(bei)數(shu)(shu)（n倍(bei)(bei)(bei)）后(hou)(hou)再(zai)(zai)測(ce)(ce)定(ding)(ding)，計(ji)算(suan)(suan)時(shi)(shi)(shi)(shi)請(qing)＊后(hou)(hou)乘以(yi)(yi)總稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)倍(bei)(bei)(bei)數(shu)(shu)（×n×5）。5． 封(feng)(feng)(feng)(feng)板(ban)(ban)膜只限一(yi)(yi)(yi)(yi)次(ci)(ci)(ci)(ci)(ci)性(xing)使(shi)(shi)用(yong)(yong)，以(yi)(yi)避(bi)免交叉(cha)污染(ran)。6． 底(di)物(wu)(wu)(wu)請(qing)避(bi)光(guang)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)。7． 嚴格按(an)(an)照(zhao)說明書的(de)操(cao)(cao)(cao)作(zuo)(zuo)進(jin)行(xing)，試(shi)(shi)(shi)(shi)(shi)驗(yan)結(jie)果判定(ding)(ding)必(bi)須以(yi)(yi)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀數(shu)(shu)為(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun).8． 所(suo)有(you)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)，洗滌(di)液(ye)(ye)(ye)(ye)和各種廢(fei)棄(qi)物(wu)(wu)(wu)都(dou)應(ying)(ying)(ying)按(an)(an)傳染(ran)物(wu)(wu)(wu)處(chu)理。9． 本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)不(bu)(bu)同(tong)(tong)(tong)批(pi)號(hao)組分(fen)(fen)(fen)(fen)不(bu)(bu)得混(hun)用(yong)(yong)。10. 如與(yu)(yu)英文(wen)說明書有(you)異(yi)，以(yi)(yi)英文(wen)說明書為(wei)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)。【計(ji)算(suan)(suan)】 以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)濃度(du)(du)(du)為(wei)橫(heng)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)為(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線，根(gen)據樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)OD 值(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線查出(chu)相應(ying)(ying)(ying)的(de)濃度(du)(du)(du)；再(zai)(zai)乘以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi) 倍(bei)(bei)(bei)數(shu)(shu)；或用(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)濃度(du)(du)(du)與(yu)(yu)OD值(zhi)計(ji)算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)線的(de)直(zhi)線回歸方程(cheng)式，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)OD值(zhi) 代入方程(cheng)式，計(ji)算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃度(du)(du)(du)，再(zai)(zai)乘以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi) 倍(bei)(bei)(bei)數(shu)(shu)，即為(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)實(shi)際濃度(du)(du)(du)。 【試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)組成】試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)組成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)說明書1份1份密(mi)封(feng)(feng)(feng)(feng)袋1個1個封(feng)(feng)(feng)(feng)板(ban)(ban)膜12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)2片（96）2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)顯(xian)色(se)劑(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)顯(xian)色(se)劑(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)終(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)注意(yi)(yi)：使(shi)(shi)用(yong)(yong)前(qian)請(qing)檢查試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)的(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和數(shu)(shu)量(liang)(liang)(liang)(liang)與(yu)(yu)表格是否(fou)一(yi)(yi)(yi)(yi)致(zhi)。【精密(mi)度(du)(du)(du)】精密(mi)度(du)(du)(du)用(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)測(ce)(ce)定(ding)(ding)值(zhi)的(de)變(bian)異(yi)系數(shu)(shu)CV 表示。CV（%） = SD/mean×100批(pi)內(nei)差(cha)(cha)：取同(tong)(tong)(tong)批(pi)次(ci)(ci)(ci)(ci)(ci)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)對(dui)低(di)、中(zhong)(zhong)(zhong)(zhong)、高(gao)(gao)值(zhi)定(ding)(ding)值(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)進(jin)行(xing)定(ding)(ding)量(liang)(liang)(liang)(liang)檢測(ce)(ce)，每(mei)(mei)份樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)連續(xu)測(ce)(ce)定(ding)(ding)20 次(ci)(ci)(ci)(ci)(ci)，分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)計(ji)算(suan)(suan)不(bu)(bu)同(tong)(tong)(tong)濃度(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)平(ping)均(jun)(jun)值(zhi)及(ji)SD 值(zhi)。批(pi)內(nei)差(cha)(cha)： CV<9%批(pi)間差(cha)(cha)：選取3 個不(bu)(bu)同(tong)(tong)(tong)批(pi)次(ci)(ci)(ci)(ci)(ci)的(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)對(dui)低(di)、中(zhong)(zhong)(zhong)(zhong)、高(gao)(gao)值(zhi)定(ding)(ding)值(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)進(jin)行(xing)定(ding)(ding)量(liang)(liang)(liang)(liang)測(ce)(ce)定(ding)(ding)，每(mei)(mei)個樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)使(shi)(shi)用(yong)(yong)同(tong)(tong)(tong)一(yi)(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)重(zhong)復測(ce)(ce)定(ding)(ding)10 次(ci)(ci)(ci)(ci)(ci)，分(fen)(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)(bie)(bie)計(ji)算(suan)(suan)不(bu)(bu)同(tong)(tong)(tong)濃度(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)平(ping)均(jun)(jun)值(zhi)及(ji)SD 值(zhi)。批(pi)間差(cha)(cha)： CV<11% 【特(te)異(yi)性(xing)】本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)識別(bie)(bie)(bie)(bie)(bie)(bie)(bie)天然(ran)和重(zhong)組微(wei)生物(wu)(wu)(wu)硝(xiao)酸鹽還原酶(mei)（Nar），經檢測(ce)(ce)與(yu)(yu)其(qi)它(ta)相似(si)物(wu)(wu)(wu)質(zhi)無明顯(xian)交叉(cha)反應(ying)(ying)(ying)。由于(yu)(yu)受到技(ji)術及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)來源的(de)限制(zhi)，不(bu)(bu)可能(neng)完成所(suo)有(you)相關或相似(si)物(wu)(wu)(wu)質(zhi)交叉(cha)反應(ying)(ying)(ying)檢測(ce)(ce)，因此(ci)(ci)本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)有(you)可能(neng)與(yu)(yu)未(wei)經檢測(ce)(ce)的(de)其(qi)它(ta)物(wu)(wu)(wu)質(zhi)有(you)交叉(cha)反應(ying)(ying)(ying)。 【穩定(ding)(ding)性(xing)】試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)在(zai)有(you)效期(qi)內(nei)務必(bi)按(an)(an)推(tui)薦(jian)溫(wen)(wen)(wen)(wen)度(du)(du)(du)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)，活(huo)性(xing)降低(di)率小于(yu)(yu)5%。為(wei)減小外部因素對(dui)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)盒(he)(he)(he)(he)(he)破壞前(qian)后(hou)(hou)檢測(ce)(ce)值(zhi)的(de)影響(xiang)，實(shi)驗(yan)室的(de)環境(jing)(jing)條(tiao)件(jian)需盡(jin)量(liang)(liang)(liang)(liang)保(bao)(bao)(bao)(bao)持一(yi)(yi)(yi)(yi)致(zhi)，尤(you)其(qi)是實(shi)驗(yan)室內(nei)溫(wen)(wen)(wen)(wen)度(du)(du)(du)、濕度(du)(du)(du)及(ji)溫(wen)(wen)(wen)(wen)育(yu)條(tiao)件(jian)。其(qi)次(ci)(ci)(ci)(ci)(ci)由同(tong)(tong)(tong)一(yi)(yi)(yi)(yi)實(shi)驗(yan)員(yuan)來進(jin)行(xing)操(cao)(cao)(cao)作(zuo)(zuo)可減少人為(wei)誤差(cha)(cha)。
牛細胞間(jian)粘(zhan)附分(fen)(fen)(fen)子3(ICAM-3/CD50) ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)(zhong)文(wen)名稱牛細胞間(jian)粘(zhan)附分(fen)(fen)(fen)子3(ICAM-3/CD50) ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)規(gui)格(ge)96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)類型(xing)血(xue)清、血(xue)漿、細胞上(shang)清液(ye)(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)(ye)、體液(ye)(ye)(ye)(ye)(ye)、灌洗(xi)液(ye)(ye)(ye)(ye)(ye)等使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)范(fan)圍科(ke)研實(shi)(shi)驗(yan)，不(bu)(bu)(bu)得用(yong)(yong)(yong)(yong)(yong)(yong)于臨床(chuang)診斷反(fan)應(ying)(ying)時間(jian)1-5h試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)保(bao)存(cun)(cun)(cun)(cun)(cun)2-8℃有(you)(you)(you)效期6個(ge)月貨期3-5天(tian)【操(cao)(cao)作(zuo)步驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)與(yu)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)(zai)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被板(ban)(ban)(ban)上(shang)設標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)(zai)第(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)100μl，然(ran)(ran)后(hou)(hou)(hou)在(zai)(zai)(zai)第(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)加(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻；然(ran)(ran)后(hou)(hou)(hou)從第(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)各取(qu)100μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)到第(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)和(he)第(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)，再在(zai)(zai)(zai)第(di)(di)(di)(di)三、第(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻；然(ran)(ran)后(hou)(hou)(hou)在(zai)(zai)(zai)第(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)和(he)第(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)先(xian)(xian)各取(qu)50μl棄(qi)(qi)掉(diao)，再各取(qu)50μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)到第(di)(di)(di)(di)五、第(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，再在(zai)(zai)(zai)第(di)(di)(di)(di)五、第(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50ul，混(hun)(hun)勻；混(hun)(hun)勻后(hou)(hou)(hou)從第(di)(di)(di)(di)五、第(di)(di)(di)(di)六(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)各取(qu)50μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)到第(di)(di)(di)(di)七、第(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，再在(zai)(zai)(zai)第(di)(di)(di)(di)七、第(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻后(hou)(hou)(hou)從第(di)(di)(di)(di)七、第(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)取(qu)50μl加(jia)(jia)到第(di)(di)(di)(di)九(jiu)、第(di)(di)(di)(di)十(shi)(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，再在(zai)(zai)(zai)第(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)十(shi)(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)加(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)勻后(hou)(hou)(hou)從第(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)十(shi)(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)各取(qu)50μl棄(qi)(qi)掉(diao)。（稀(xi)(xi)(xi)釋(shi)(shi)后(hou)(hou)(hou)各孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)都為(wei)(wei)50μl，濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)為(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)設空(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)白對(dui)(dui)照(zhao)孔(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)及(ji)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，其(qi)(qi)(qi)余各步操(cao)(cao)作(zuo)相(xiang)(xiang)(xiang)同(tong)(tong)(tong)）、待(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)(zai)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被板(ban)(ban)(ban)上(shang)待(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)先(xian)(xian)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)40μl，然(ran)(ran)后(hou)(hou)(hou)再加(jia)(jia)待(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)＊終(zhong)(zhong)稀(xi)(xi)(xi)釋(shi)(shi)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)5倍(bei)）。加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)加(jia)(jia)于酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)底(di)部(bu)，盡(jin)量(liang)(liang)不(bu)(bu)(bu)觸及(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)輕(qing)晃(huang)動混(hun)(hun)勻。3. 溫(wen)(wen)(wen)育(yu)(yu)：用(yong)(yong)(yong)(yong)(yong)(yong)封板(ban)(ban)(ban)膜(mo)封板(ban)(ban)(ban)后(hou)(hou)(hou)置37℃溫(wen)(wen)(wen)育(yu)(yu)30分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。4. 配(pei)液(ye)(ye)(ye)(ye)(ye)：將30（48T的(de)(de)(de)(de)(de)20倍(bei)）倍(bei)濃(nong)(nong)(nong)縮洗(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)用(yong)(yong)(yong)(yong)(yong)(yong)蒸(zheng)餾水(shui)30（48T的(de)(de)(de)(de)(de)20倍(bei)）倍(bei)稀(xi)(xi)(xi)釋(shi)(shi)后(hou)(hou)(hou)備用(yong)(yong)(yong)(yong)(yong)(yong)。5. 洗(xi)滌(di)：小心揭掉(diao)封板(ban)(ban)(ban)膜(mo)，棄(qi)(qi)去液(ye)(ye)(ye)(ye)(ye)體，甩干，每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)滿洗(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)，靜置30秒后(hou)(hou)(hou)棄(qi)(qi)去，如(ru)此(ci)重(zhong)復5次(ci)(ci)，拍干。6. 加(jia)(jia)酶(mei)(mei)(mei)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)入(ru)(ru)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)50μl，空(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)除外。7. 溫(wen)(wen)(wen)育(yu)(yu)：操(cao)(cao)作(zuo)同(tong)(tong)(tong)3。8. 洗(xi)滌(di)：操(cao)(cao)作(zuo)同(tong)(tong)(tong)5。9. 顯(xian)(xian)色(se)(se)(se)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)先(xian)(xian)加(jia)(jia)入(ru)(ru)顯(xian)(xian)色(se)(se)(se)劑(ji)(ji)(ji)(ji)A50μl，再加(jia)(jia)入(ru)(ru)顯(xian)(xian)色(se)(se)(se)劑(ji)(ji)(ji)(ji)B50μl，輕(qing)輕(qing)震(zhen)蕩(dang)混(hun)(hun)勻，37℃避光顯(xian)(xian)色(se)(se)(se)15分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong). 10. 終(zhong)(zhong)止(zhi)(zhi)(zhi)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)終(zhong)(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)止(zhi)(zhi)(zhi)反(fan)應(ying)(ying)（此(ci)時藍色(se)(se)(se)立轉黃色(se)(se)(se)）。11. 測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)：以(yi)(yi)空(kong)白空(kong)調(diao)零(ling)，450nm波長依序(xu)測(ce)(ce)(ce)量(liang)(liang)各孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)吸光度(du)(du)(du)(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)應(ying)(ying)在(zai)(zai)(zai)加(jia)(jia)終(zhong)(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)內(nei)(nei)(nei)進(jin)行(xing)(xing)(xing)。牛細胞間(jian)粘(zhan)附分(fen)(fen)(fen)子3(ICAM-3/CD50) ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)【注(zhu)意事項(xiang)】1． 試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)從冷藏環境中(zhong)(zhong)(zhong)取(qu)出(chu)(chu)應(ying)(ying)在(zai)(zai)(zai)室溫(wen)(wen)(wen)平(ping)衡15-30分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)方可使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)，酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被板(ban)(ban)(ban)開封后(hou)(hou)(hou)如(ru)未用(yong)(yong)(yong)(yong)(yong)(yong)完，板(ban)(ban)(ban)條(tiao)(tiao)應(ying)(ying)裝入(ru)(ru)密(mi)封袋中(zhong)(zhong)(zhong)保(bao)存(cun)(cun)(cun)(cun)(cun)。2． 濃(nong)(nong)(nong)洗(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)可能會有(you)(you)(you)結(jie)晶析出(chu)(chu)，稀(xi)(xi)(xi)釋(shi)(shi)時可在(zai)(zai)(zai)水(shui)浴(yu)中(zhong)(zhong)(zhong)加(jia)(jia)溫(wen)(wen)(wen)助溶(rong)，洗(xi)滌(di)時不(bu)(bu)(bu)影響結(jie)果(guo)。3． 各步加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)均應(ying)(ying)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)器，并(bing)經(jing)常校對(dui)(dui)其(qi)(qi)(qi)準(zhun)(zhun)確性，以(yi)(yi)避免試(shi)(shi)(shi)(shi)驗(yan)誤差(cha)。一(yi)(yi)次(ci)(ci)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時間(jian)＊好控制在(zai)(zai)(zai)5分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)數量(liang)(liang)多，推薦使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4． 請(qing)每(mei)(mei)次(ci)(ci)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)同(tong)(tong)(tong)時做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲線，＊好做(zuo)復孔(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)中(zhong)(zhong)(zhong)待(dai)測(ce)(ce)(ce)物(wu)(wu)質(zhi)(zhi)(zhi)含量(liang)(liang)過(guo)高（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)OD值(zhi)(zhi)(zhi)(zhi)(zhi)大于標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)），請(qing)先(xian)(xian)用(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)釋(shi)(shi)一(yi)(yi)定(ding)(ding)(ding)(ding)倍(bei)數（n倍(bei)）后(hou)(hou)(hou)再測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)，計(ji)(ji)算(suan)(suan)時請(qing)＊后(hou)(hou)(hou)乘以(yi)(yi)總稀(xi)(xi)(xi)釋(shi)(shi)倍(bei)數（×n×5）。5． 封板(ban)(ban)(ban)膜(mo)只(zhi)限一(yi)(yi)次(ci)(ci)性使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)，以(yi)(yi)避免交(jiao)叉(cha)污染(ran)。6． 底(di)物(wu)(wu)請(qing)避光保(bao)存(cun)(cun)(cun)(cun)(cun)。7． 嚴格(ge)按照(zhao)說(shuo)明書(shu)(shu)的(de)(de)(de)(de)(de)操(cao)(cao)作(zuo)進(jin)行(xing)(xing)(xing)，試(shi)(shi)(shi)(shi)驗(yan)結(jie)果(guo)判定(ding)(ding)(ding)(ding)必(bi)須以(yi)(yi)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀數為(wei)(wei)準(zhun)(zhun).8． 所有(you)(you)(you)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)，洗(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)和(he)各種(zhong)廢棄(qi)(qi)物(wu)(wu)都應(ying)(ying)按傳染(ran)物(wu)(wu)處理。9． 本(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)不(bu)(bu)(bu)同(tong)(tong)(tong)批(pi)號組(zu)分(fen)(fen)(fen)不(bu)(bu)(bu)得混(hun)(hun)用(yong)(yong)(yong)(yong)(yong)(yong)。10. 如(ru)與(yu)英文(wen)說(shuo)明書(shu)(shu)有(you)(you)(you)異(yi)，以(yi)(yi)英文(wen)說(shuo)明書(shu)(shu)為(wei)(wei)準(zhun)(zhun)。【計(ji)(ji)算(suan)(suan)】 以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)為(wei)(wei)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)繪出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲線，根據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)OD 值(zhi)(zhi)(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲線查(cha)出(chu)(chu)相(xiang)(xiang)(xiang)應(ying)(ying)的(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)；再乘以(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi) 倍(bei)數；或(huo)用(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)(zhi)(zhi)計(ji)(ji)算(suan)(suan)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)曲線的(de)(de)(de)(de)(de)直線回歸方程式(shi)，將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi) 代入(ru)(ru)方程式(shi)，計(ji)(ji)算(suan)(suan)出(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)，再乘以(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi) 倍(bei)數，即為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)實(shi)(shi)際濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)。 【試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)組(zu)成(cheng)】試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)組(zu)成(cheng)48孔(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置96孔(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置保(bao)存(cun)(cun)(cun)(cun)(cun)說(shuo)明書(shu)(shu)1份(fen)1份(fen)密(mi)封袋1個(ge)1個(ge)封板(ban)(ban)(ban)膜(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)(tiao)2片（96）2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)(tiao)2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)1.5ml×1瓶(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)顯(xian)(xian)色(se)(se)(se)劑(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)顯(xian)(xian)色(se)(se)(se)劑(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)終(zhong)(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)3ml×1瓶(ping)6ml×1瓶(ping)2-8℃保(bao)存(cun)(cun)(cun)(cun)(cun)注(zhu)意：使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)前請(qing)檢查(cha)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽(qian)和(he)數量(liang)(liang)與(yu)表格(ge)是否一(yi)(yi)致。【精密(mi)度(du)(du)(du)(du)(du)(du)(du)】精密(mi)度(du)(du)(du)(du)(du)(du)(du)用(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)變異(yi)系(xi)數CV 表示。CV（%） = SD/mean×100批(pi)內(nei)(nei)(nei)差(cha)：取(qu)同(tong)(tong)(tong)批(pi)次(ci)(ci)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)對(dui)(dui)低、中(zhong)(zhong)(zhong)、高值(zhi)(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)進(jin)行(xing)(xing)(xing)定(ding)(ding)(ding)(ding)量(liang)(liang)檢測(ce)(ce)(ce)，每(mei)(mei)份(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)連續測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)20 次(ci)(ci)，分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)計(ji)(ji)算(suan)(suan)不(bu)(bu)(bu)同(tong)(tong)(tong)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)(de)(de)(de)平(ping)均值(zhi)(zhi)(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)(zhi)(zhi)。批(pi)內(nei)(nei)(nei)差(cha)： CV<9%批(pi)間(jian)差(cha)：選取(qu)3 個(ge)不(bu)(bu)(bu)同(tong)(tong)(tong)批(pi)次(ci)(ci)的(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)對(dui)(dui)低、中(zhong)(zhong)(zhong)、高值(zhi)(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)進(jin)行(xing)(xing)(xing)定(ding)(ding)(ding)(ding)量(liang)(liang)測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)，每(mei)(mei)個(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)同(tong)(tong)(tong)一(yi)(yi)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)重(zhong)復測(ce)(ce)(ce)定(ding)(ding)(ding)(ding)10 次(ci)(ci)，分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)(bie)計(ji)(ji)算(suan)(suan)不(bu)(bu)(bu)同(tong)(tong)(tong)濃(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)的(de)(de)(de)(de)(de)平(ping)均值(zhi)(zhi)(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)(zhi)(zhi)。批(pi)間(jian)差(cha)： CV<11% 【特(te)異(yi)性】本(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)識別(bie)(bie)(bie)(bie)(bie)天(tian)然(ran)(ran)和(he)重(zhong)組(zu)微(wei)生物(wu)(wu)硝酸鹽還原酶(mei)(mei)(mei)（Nar），經(jing)檢測(ce)(ce)(ce)與(yu)其(qi)(qi)(qi)它(ta)相(xiang)(xiang)(xiang)似(si)物(wu)(wu)質(zhi)(zhi)(zhi)無明顯(xian)(xian)交(jiao)叉(cha)反(fan)應(ying)(ying)。由(you)于受到技(ji)術及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)來源的(de)(de)(de)(de)(de)限制，不(bu)(bu)(bu)可能完成(cheng)所有(you)(you)(you)相(xiang)(xiang)(xiang)關或(huo)相(xiang)(xiang)(xiang)似(si)物(wu)(wu)質(zhi)(zhi)(zhi)交(jiao)叉(cha)反(fan)應(ying)(ying)檢測(ce)(ce)(ce)，因此(ci)本(ben)(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)有(you)(you)(you)可能與(yu)未經(jing)檢測(ce)(ce)(ce)的(de)(de)(de)(de)(de)其(qi)(qi)(qi)它(ta)物(wu)(wu)質(zhi)(zhi)(zhi)有(you)(you)(you)交(jiao)叉(cha)反(fan)應(ying)(ying)。 【穩定(ding)(ding)(ding)(ding)性】試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)在(zai)(zai)(zai)有(you)(you)(you)效期內(nei)(nei)(nei)務必(bi)按推薦溫(wen)(wen)(wen)度(du)(du)(du)(du)(du)(du)(du)保(bao)存(cun)(cun)(cun)(cun)(cun)，活性降(jiang)低率小于5%。為(wei)(wei)減小外部(bu)因素(su)對(dui)(dui)試(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)破(po)壞(huai)前后(hou)(hou)(hou)檢測(ce)(ce)(ce)值(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)影響，實(shi)(shi)驗(yan)室的(de)(de)(de)(de)(de)環境條(tiao)(tiao)件需盡(jin)量(liang)(liang)保(bao)持(chi)一(yi)(yi)致，尤其(qi)(qi)(qi)是實(shi)(shi)驗(yan)室內(nei)(nei)(nei)溫(wen)(wen)(wen)度(du)(du)(du)(du)(du)(du)(du)、濕(shi)度(du)(du)(du)(du)(du)(du)(du)及(ji)溫(wen)(wen)(wen)育(yu)(yu)條(tiao)(tiao)件。其(qi)(qi)(qi)次(ci)(ci)由(you)同(tong)(tong)(tong)一(yi)(yi)實(shi)(shi)驗(yan)員來進(jin)行(xing)(xing)(xing)操(cao)(cao)作(zuo)可減少人為(wei)(wei)誤差(cha)。
牛黃(huang)嘌(piao)(piao)呤(ling)氧(yang)化(hua)酶(mei)(mei)(mei)(mei)(XOD) ELISA 試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)文(wen)名稱牛黃(huang)嘌(piao)(piao)呤(ling)氧(yang)化(hua)酶(mei)(mei)(mei)(mei)(XOD) ELISA 試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)規(gui)格96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)類型血清、血漿、細胞上清液(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)、體液(ye)(ye)(ye)(ye)、灌洗(xi)(xi)(xi)(xi)液(ye)(ye)(ye)(ye)等使(shi)(shi)用(yong)范圍科研(yan)實(shi)(shi)驗(yan)，不(bu)(bu)得(de)用(yong)于(yu)臨床診(zhen)斷反(fan)(fan)應(ying)時(shi)(shi)(shi)(shi)間(jian)1-5h試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)2-8℃有(you)(you)效期(qi)(qi)6個(ge)月貨期(qi)(qi)3-5天(tian)【操(cao)作(zuo)步(bu)驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)釋(shi)(shi)(shi)與(yu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：在酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)(ban)(ban)上設(she)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在第(di)(di)(di)(di)一(yi)、第(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)100μl，然(ran)(ran)(ran)后(hou)(hou)(hou)(hou)(hou)(hou)在第(di)(di)(di)(di)一(yi)、第(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混勻(yun)(yun)；然(ran)(ran)(ran)后(hou)(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)一(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)(ge)取(qu)(qu)100μl分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)第(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)在第(di)(di)(di)(di)三、第(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混勻(yun)(yun)；然(ran)(ran)(ran)后(hou)(hou)(hou)(hou)(hou)(hou)在第(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)第(di)(di)(di)(di)四孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)先各(ge)(ge)(ge)取(qu)(qu)50μl棄掉，再(zai)(zai)各(ge)(ge)(ge)取(qu)(qu)50μl分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在第(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)50ul，混勻(yun)(yun)；混勻(yun)(yun)后(hou)(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)(ge)取(qu)(qu)50μl分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)七、第(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在第(di)(di)(di)(di)七、第(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混勻(yun)(yun)后(hou)(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)七、第(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)分(fen)(fen)別(bie)(bie)(bie)取(qu)(qu)50μl加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到第(di)(di)(di)(di)九(jiu)、第(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)，再(zai)(zai)在第(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)別(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混勻(yun)(yun)后(hou)(hou)(hou)(hou)(hou)(hou)從(cong)第(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)各(ge)(ge)(ge)取(qu)(qu)50μl棄掉。（稀(xi)(xi)釋(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)(hou)各(ge)(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量都為(wei)(wei)(wei)(wei)50μl，濃(nong)度(du)(du)(du)分(fen)(fen)別(bie)(bie)(bie)為(wei)(wei)(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)別(bie)(bie)(bie)設(she)空(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)白對(dui)照孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)及(ji)(ji)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，其(qi)余各(ge)(ge)(ge)步(bu)操(cao)作(zuo)相同(tong)）、待(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)(ban)(ban)上待(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)(zhong)先加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)40μl，然(ran)(ran)(ran)后(hou)(hou)(hou)(hou)(hou)(hou)再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)待(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)＊終(zhong)稀(xi)(xi)釋(shi)(shi)(shi)度(du)(du)(du)為(wei)(wei)(wei)(wei)5倍(bei)(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部，盡量不(bu)(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)輕(qing)晃動混勻(yun)(yun)。3. 溫育(yu)：用(yong)封(feng)板(ban)(ban)(ban)膜(mo)封(feng)板(ban)(ban)(ban)后(hou)(hou)(hou)(hou)(hou)(hou)置(zhi)37℃溫育(yu)30分(fen)(fen)鐘(zhong)(zhong)。4. 配液(ye)(ye)(ye)(ye)：將(jiang)30（48T的(de)(de)(de)(de)(de)(de)(de)(de)20倍(bei)(bei)(bei)）倍(bei)(bei)(bei)濃(nong)縮洗(xi)(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)用(yong)蒸餾水(shui)30（48T的(de)(de)(de)(de)(de)(de)(de)(de)20倍(bei)(bei)(bei)）倍(bei)(bei)(bei)稀(xi)(xi)釋(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)(hou)備用(yong)。5. 洗(xi)(xi)(xi)(xi)滌(di)(di)：小(xiao)(xiao)心揭掉封(feng)板(ban)(ban)(ban)膜(mo)，棄去(qu)液(ye)(ye)(ye)(ye)體，甩干(gan)，每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)，靜(jing)置(zhi)30秒后(hou)(hou)(hou)(hou)(hou)(hou)棄去(qu)，如(ru)此重復(fu)5次(ci)，拍干(gan)。6. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)(mei)(mei)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)50μl，空(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外。7. 溫育(yu)：操(cao)作(zuo)同(tong)3。8. 洗(xi)(xi)(xi)(xi)滌(di)(di)：操(cao)作(zuo)同(tong)5。9. 顯(xian)(xian)色(se)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)顯(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)A50μl，再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)顯(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)B50μl，輕(qing)輕(qing)震蕩混勻(yun)(yun)，37℃避光(guang)顯(xian)(xian)色(se)15分(fen)(fen)鐘(zhong)(zhong). 10. 終(zhong)止(zhi)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)液(ye)(ye)(ye)(ye)50μl，終(zhong)止(zhi)反(fan)(fan)應(ying)（此時(shi)(shi)(shi)(shi)藍(lan)色(se)立(li)轉黃(huang)色(se)）。11. 測(ce)(ce)(ce)定(ding)(ding)：以空(kong)白空(kong)調(diao)零，450nm波(bo)長依序測(ce)(ce)(ce)量各(ge)(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)吸光(guang)度(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)定(ding)(ding)應(ying)在加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)液(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)(hou)(hou)15分(fen)(fen)鐘(zhong)(zhong)以內(nei)進(jin)行(xing)。牛黃(huang)嘌(piao)(piao)呤(ling)氧(yang)化(hua)酶(mei)(mei)(mei)(mei)(XOD) ELISA 試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)【注意事項】1． 試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)從(cong)冷藏環境(jing)中(zhong)(zhong)(zhong)(zhong)(zhong)取(qu)(qu)出(chu)應(ying)在室(shi)溫平衡15-30分(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)(hou)(hou)(hou)(hou)方(fang)可使(shi)(shi)用(yong)，酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包(bao)被(bei)板(ban)(ban)(ban)開封(feng)后(hou)(hou)(hou)(hou)(hou)(hou)如(ru)未用(yong)完(wan)，板(ban)(ban)(ban)條應(ying)裝(zhuang)入(ru)密封(feng)袋中(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。2． 濃(nong)洗(xi)(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)可能(neng)(neng)會有(you)(you)結晶(jing)析出(chu)，稀(xi)(xi)釋(shi)(shi)(shi)時(shi)(shi)(shi)(shi)可在水(shui)浴中(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)溫助溶，洗(xi)(xi)(xi)(xi)滌(di)(di)時(shi)(shi)(shi)(shi)不(bu)(bu)影響結果。3． 各(ge)(ge)(ge)步(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)均(jun)應(ying)使(shi)(shi)用(yong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)器，并經(jing)常校對(dui)其(qi)準(zhun)(zhun)確性，以避免(mian)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)誤(wu)差。一(yi)次(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)間(jian)＊好控制(zhi)在5分(fen)(fen)鐘(zhong)(zhong)內(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)數(shu)量多，推薦(jian)(jian)使(shi)(shi)用(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4． 請(qing)(qing)每(mei)次(ci)測(ce)(ce)(ce)定(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi)(shi)(shi)做(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)線(xian)，＊好做(zuo)(zuo)復(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)待(dai)測(ce)(ce)(ce)物質含(han)量過高（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)OD值(zhi)(zhi)(zhi)(zhi)大于(yu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)一(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)），請(qing)(qing)先用(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)稀(xi)(xi)釋(shi)(shi)(shi)一(yi)定(ding)(ding)倍(bei)(bei)(bei)數(shu)（n倍(bei)(bei)(bei)）后(hou)(hou)(hou)(hou)(hou)(hou)再(zai)(zai)測(ce)(ce)(ce)定(ding)(ding)，計(ji)(ji)算(suan)(suan)時(shi)(shi)(shi)(shi)請(qing)(qing)＊后(hou)(hou)(hou)(hou)(hou)(hou)乘以總(zong)稀(xi)(xi)釋(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)（×n×5）。5． 封(feng)板(ban)(ban)(ban)膜(mo)只(zhi)限一(yi)次(ci)性使(shi)(shi)用(yong)，以避免(mian)交(jiao)叉(cha)(cha)污染(ran)。6． 底物請(qing)(qing)避光(guang)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。7． 嚴格按(an)照說(shuo)明(ming)書的(de)(de)(de)(de)(de)(de)(de)(de)操(cao)作(zuo)進(jin)行(xing)，試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)結果判定(ding)(ding)必須(xu)以酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀數(shu)為(wei)(wei)(wei)(wei)準(zhun)(zhun).8． 所(suo)有(you)(you)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)，洗(xi)(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)(ye)和(he)(he)各(ge)(ge)(ge)種廢棄物都應(ying)按(an)傳(chuan)染(ran)物處理。9． 本(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)不(bu)(bu)同(tong)批(pi)號組(zu)分(fen)(fen)不(bu)(bu)得(de)混用(yong)。10. 如(ru)與(yu)英文(wen)說(shuo)明(ming)書有(you)(you)異(yi)(yi)，以英文(wen)說(shuo)明(ming)書為(wei)(wei)(wei)(wei)準(zhun)(zhun)。【計(ji)(ji)算(suan)(suan)】 以標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)物的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)為(wei)(wei)(wei)(wei)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)， 在坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)線(xian)，根據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)OD 值(zhi)(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)(qu)線(xian)查(cha)(cha)出(chu)相應(ying)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)；再(zai)(zai)乘以稀(xi)(xi)釋(shi)(shi)(shi) 倍(bei)(bei)(bei)數(shu)；或用(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)物的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)(zhi)計(ji)(ji)算(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)曲(qu)(qu)線(xian)的(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)回歸方(fang)程(cheng)式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi) 代入(ru)方(fang)程(cheng)式(shi)，計(ji)(ji)算(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)度(du)(du)(du)，再(zai)(zai)乘以稀(xi)(xi)釋(shi)(shi)(shi) 倍(bei)(bei)(bei)數(shu)，即為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際(ji)濃(nong)度(du)(du)(du)。 【試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)組(zu)成】試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)組(zu)成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)說(shuo)明(ming)書1份(fen)1份(fen)密封(feng)袋1個(ge)1個(ge)封(feng)板(ban)(ban)(ban)膜(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條2片（96）2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)酶(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)釋(shi)(shi)(shi)液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)顯(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)顯(xian)(xian)色(se)劑(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)終(zhong)止(zhi)液(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)注意：使(shi)(shi)用(yong)前(qian)請(qing)(qing)檢(jian)(jian)查(cha)(cha)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和(he)(he)數(shu)量與(yu)表格是否一(yi)致(zhi)。【精密度(du)(du)(du)】精密度(du)(du)(du)用(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)定(ding)(ding)值(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)變異(yi)(yi)系數(shu)CV 表示。CV（%） = SD/mean×100批(pi)內(nei)差：取(qu)(qu)同(tong)批(pi)次(ci)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)對(dui)低、中(zhong)(zhong)(zhong)(zhong)(zhong)、高值(zhi)(zhi)(zhi)(zhi)定(ding)(ding)值(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)進(jin)行(xing)定(ding)(ding)量檢(jian)(jian)測(ce)(ce)(ce)，每(mei)份(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)連續測(ce)(ce)(ce)定(ding)(ding)20 次(ci)，分(fen)(fen)別(bie)(bie)(bie)計(ji)(ji)算(suan)(suan)不(bu)(bu)同(tong)濃(nong)度(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)的(de)(de)(de)(de)(de)(de)(de)(de)平均(jun)值(zhi)(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)(zhi)。批(pi)內(nei)差： CV<9%批(pi)間(jian)差：選取(qu)(qu)3 個(ge)不(bu)(bu)同(tong)批(pi)次(ci)的(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)分(fen)(fen)別(bie)(bie)(bie)對(dui)低、中(zhong)(zhong)(zhong)(zhong)(zhong)、高值(zhi)(zhi)(zhi)(zhi)定(ding)(ding)值(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)進(jin)行(xing)定(ding)(ding)量測(ce)(ce)(ce)定(ding)(ding)，每(mei)個(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)使(shi)(shi)用(yong)同(tong)一(yi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)重復(fu)測(ce)(ce)(ce)定(ding)(ding)10 次(ci)，分(fen)(fen)別(bie)(bie)(bie)計(ji)(ji)算(suan)(suan)不(bu)(bu)同(tong)濃(nong)度(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)的(de)(de)(de)(de)(de)(de)(de)(de)平均(jun)值(zhi)(zhi)(zhi)(zhi)及(ji)(ji)SD 值(zhi)(zhi)(zhi)(zhi)。批(pi)間(jian)差： CV<11% 【特異(yi)(yi)性】本(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)識別(bie)(bie)(bie)天(tian)然(ran)(ran)(ran)和(he)(he)重組(zu)微生物硝酸鹽還原酶(mei)(mei)(mei)(mei)（Nar），經(jing)檢(jian)(jian)測(ce)(ce)(ce)與(yu)其(qi)它相似物質無明(ming)顯(xian)(xian)交(jiao)叉(cha)(cha)反(fan)(fan)應(ying)。由于(yu)受到技術(shu)及(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)來(lai)源(yuan)的(de)(de)(de)(de)(de)(de)(de)(de)限制(zhi)，不(bu)(bu)可能(neng)(neng)完(wan)成所(suo)有(you)(you)相關或相似物質交(jiao)叉(cha)(cha)反(fan)(fan)應(ying)檢(jian)(jian)測(ce)(ce)(ce)，因此本(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)有(you)(you)可能(neng)(neng)與(yu)未經(jing)檢(jian)(jian)測(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)其(qi)它物質有(you)(you)交(jiao)叉(cha)(cha)反(fan)(fan)應(ying)。 【穩定(ding)(ding)性】試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)在有(you)(you)效期(qi)(qi)內(nei)務必按(an)推薦(jian)(jian)溫度(du)(du)(du)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)，活性降低率小(xiao)(xiao)于(yu)5%。為(wei)(wei)(wei)(wei)減小(xiao)(xiao)外部因素(su)對(dui)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)破壞前(qian)后(hou)(hou)(hou)(hou)(hou)(hou)檢(jian)(jian)測(ce)(ce)(ce)值(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)影響，實(shi)(shi)驗(yan)室(shi)的(de)(de)(de)(de)(de)(de)(de)(de)環境(jing)條件(jian)需盡量保(bao)(bao)(bao)(bao)持一(yi)致(zhi)，尤其(qi)是實(shi)(shi)驗(yan)室(shi)內(nei)溫度(du)(du)(du)、濕度(du)(du)(du)及(ji)(ji)溫育(yu)條件(jian)。其(qi)次(ci)由同(tong)一(yi)實(shi)(shi)驗(yan)員來(lai)進(jin)行(xing)操(cao)作(zuo)可減少(shao)人為(wei)(wei)(wei)(wei)誤(wu)差。
僅供(gong)體(ti)(ti)(ti)(ti)外(wai)(wai)研(yan)究使用(yong)(yong)，不(bu)用(yong)(yong)于(yu)(yu)臨(lin)床診斷!牛(niu)雙(shuang)載(zai)(zai)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(AMPH) ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)盒牛(niu)雙(shuang)載(zai)(zai)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(AMPH) ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)盒[樣(yang)(yang)(yang)(yang)(yang)本(ben)處理及(ji)要(yao)(yao)求(qiu)]1、血(xue)(xue)清(qing)：將(jiang)(jiang)(jiang)收集(ji)(ji)于(yu)(yu)血(xue)(xue)清(qing)分(fen)(fen)離(li)(li)管(guan)(guan)的(de)(de)(de)全血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)本(ben)在室(shi)溫(wen)(wen)放置(zhi)(zhi)2小(xiao)時或(huo)4℃過夜，然后(hou)(hou)(hou)1000×g離(li)(li)心(xin)20 分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，取(qu)上(shang)清(qing)即(ji)(ji)可，或(huo)將(jiang)(jiang)(jiang)上(shang)清(qing)置(zhi)(zhi)于(yu)(yu)-20℃或(huo)-80℃保(bao)存(cun)，但應(ying)(ying)避(bi)(bi)免反(fan)復(fu)凍(dong)融(rong)(rong)。2、血(xue)(xue)漿(jiang)：用(yong)(yong)EDTA或(huo)肝素作(zuo)(zuo)為抗(kang)凝劑(ji)(ji)采(cai)集(ji)(ji)標(biao)(biao)(biao)(biao)(biao)本(ben)，并(bing)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)本(ben)在采(cai)集(ji)(ji)后(hou)(hou)(hou)的(de)(de)(de)30分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)于(yu)(yu)2-8℃ 1000×g離(li)(li)心(xin)15分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，取(qu)上(shang)清(qing)即(ji)(ji)可檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)，或(huo)將(jiang)(jiang)(jiang)上(shang)清(qing)置(zhi)(zhi)于(yu)(yu)-20℃或(huo)-80℃保(bao)存(cun)，但應(ying)(ying)避(bi)(bi)免反(fan)復(fu)凍(dong)融(rong)(rong)。3、組織(zhi)(zhi)勻(yun)(yun)漿(jiang)：用(yong)(yong)預(yu)冷的(de)(de)(de)PBS (0.01M, pH=7.4)沖洗(xi)(xi)組織(zhi)(zhi)，去(qu)(qu)除殘留血(xue)(xue)液（勻(yun)(yun)漿(jiang)中(zhong)裂(lie)解(jie)的(de)(de)(de)紅(hong)細胞(bao)(bao)會影響測(ce)(ce)(ce)(ce)量(liang)(liang)結(jie)果），稱重(zhong)后(hou)(hou)(hou)將(jiang)(jiang)(jiang)組織(zhi)(zhi)剪碎。將(jiang)(jiang)(jiang)剪碎的(de)(de)(de)組織(zhi)(zhi)與對(dui)應(ying)(ying)體(ti)(ti)(ti)(ti)積(ji)的(de)(de)(de)PBS（一(yi)般按(an)1:9的(de)(de)(de)重(zhong)量(liang)(liang)體(ti)(ti)(ti)(ti)積(ji)比，比如1g的(de)(de)(de)組織(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)對(dui)應(ying)(ying)9mL的(de)(de)(de)PBS，具體(ti)(ti)(ti)(ti)體(ti)(ti)(ti)(ti)積(ji)可根據實驗(yan)需(xu)要(yao)(yao)適當(dang)調(diao)整，并(bing)做(zuo)好記錄(lu)。推薦在PBS中(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)酶抑(yi)制劑(ji)(ji)）加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)玻璃勻(yun)(yun)漿(jiang)器中(zhong)，于(yu)(yu)冰上(shang)充分(fen)(fen)研(yan)磨(mo)。為了進(jin)(jin)一(yi)步裂(lie)解(jie)組織(zhi)(zhi)細胞(bao)(bao)，可以(yi)對(dui)勻(yun)(yun)漿(jiang)液進(jin)(jin)行(xing)(xing)超聲(sheng)破碎，或(huo)反(fan)復(fu)凍(dong)融(rong)(rong)。后(hou)(hou)(hou)將(jiang)(jiang)(jiang)勻(yun)(yun)漿(jiang)液于(yu)(yu)5000×g離(li)(li)心(xin)5~10分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，取(qu)上(shang)清(qing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)。4、細胞(bao)(bao)培養(yang)物上(shang)清(qing)或(huo)其它生物標(biao)(biao)(biao)(biao)(biao)本(ben)：請1000×g離(li)(li)心(xin)20分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，取(qu)上(shang)清(qing)即(ji)(ji)可檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)，或(huo)將(jiang)(jiang)(jiang)上(shang)清(qing)置(zhi)(zhi)于(yu)(yu)-20℃或(huo)-80℃保(bao)存(cun)，但應(ying)(ying)避(bi)(bi)免反(fan)復(fu)凍(dong)融(rong)(rong)。注：標(biao)(biao)(biao)(biao)(biao)本(ben)溶(rong)血(xue)(xue)會影響后(hou)(hou)(hou)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)結(jie)果，因此(ci)溶(rong)血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)本(ben)不(bu)宜進(jin)(jin)行(xing)(xing)此(ci)項(xiang)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)。牛(niu)雙(shuang)載(zai)(zai)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(AMPH) ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)盒[試(shi)(shi)(shi)(shi)劑(ji)(ji)準(zhun)(zhun)備(bei)]實驗(yan)操作(zuo)(zuo)從冷藏(zang)環境中(zhong)取(qu)出應(ying)(ying)在室(shi)溫(wen)(wen)平衡后(hou)(hou)(hou)方(fang)可使用(yong)(yong)。20×洗(xi)(xi)滌(di)(di)(di)緩沖液的(de)(de)(de)稀(xi)釋(shi)：蒸餾水(shui)按(an)1：20稀(xi)釋(shi)，即(ji)(ji)1份20×洗(xi)(xi)滌(di)(di)(di)緩沖液加(jia)(jia)(jia)(jia)(jia)(jia)19份蒸餾水(shui)。[操作(zuo)(zuo)步驟]1、從室(shi)溫(wen)(wen)平衡20min后(hou)(hou)(hou)的(de)(de)(de)鋁箔(bo)袋(dai)中(zhong)取(qu)出所需(xu)板(ban)條(tiao)，剩余(yu)(yu)板(ban)條(tiao)用(yong)(yong)自封(feng)(feng)袋(dai)密封(feng)(feng)放回(hui)4℃。2、設置(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)樣(yang)(yang)(yang)(yang)(yang)本(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)各(ge)加(jia)(jia)(jia)(jia)(jia)(jia)不(bu)同(tong)(tong)濃(nong)度的(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)50μL；3、樣(yang)(yang)(yang)(yang)(yang)本(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)待(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)本(ben)50μL；空(kong)白(bai)(bai)(bai)(bai)(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)(jia)(jia)(jia)(jia)。4、除空(kong)白(bai)(bai)(bai)(bai)(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)外(wai)(wai)，標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)樣(yang)(yang)(yang)(yang)(yang)本(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)辣根過氧化物酶（HRP）標(biao)(biao)(biao)(biao)(biao)記的(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)抗(kang)體(ti)(ti)(ti)(ti)100μL，用(yong)(yong)封(feng)(feng)板(ban)膜封(feng)(feng)住反(fan)應(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，37℃水(shui)浴(yu)鍋(guo)或(huo)恒溫(wen)(wen)箱溫(wen)(wen)育60min。5、棄(qi)去(qu)(qu)液體(ti)(ti)(ti)(ti)，吸水(shui)紙(zhi)上(shang)拍干(gan)，每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)滿洗(xi)(xi)滌(di)(di)(di)液（350μL），靜置(zhi)(zhi)1min，甩(shuai)去(qu)(qu)洗(xi)(xi)滌(di)(di)(di)液，吸水(shui)紙(zhi)上(shang)拍干(gan)，如此(ci)重(zhong)復(fu)洗(xi)(xi)板(ban)5次（也(ye)可用(yong)(yong)洗(xi)(xi)板(ban)機洗(xi)(xi)板(ban)）。6、每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)底(di)物A、B各(ge)50μL，37℃避(bi)(bi)光(guang)孵(fu)育15min。7、每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)終(zhong)止液50μL，15min內(nei)(nei)，在450nm波(bo)長(chang)處測(ce)(ce)(ce)(ce)定(ding)(ding)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)OD值。 牛(niu)雙(shuang)載(zai)(zai)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(AMPH) ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)盒[操作(zuo)(zuo)步驟]1.標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)的(de)(de)(de)稀(xi)釋(shi)：本(ben)試(shi)(shi)(shi)(shi)劑(ji)(ji)盒提(ti)供(gong)原倍(bei)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)一(yi)支，用(yong)(yong)戶可按(an)照隨貨(huo)說(shuo)明(ming)書中(zhong)圖表在小(xiao)試(shi)(shi)(shi)(shi)管(guan)(guan)中(zhong)進(jin)(jin)行(xing)(xing)稀(xi)釋(shi)。2.加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)別設空(kong)白(bai)(bai)(bai)(bai)(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)白(bai)(bai)(bai)(bai)(bai)(bai)對(dui)照孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)及(ji)酶標(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)，其余(yu)(yu)各(ge)步操作(zuo)(zuo)相同(tong)(tong)）、標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、待(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在酶標(biao)(biao)(biao)(biao)(biao)包被板(ban)上(shang)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)準(zhun)(zhun)確(que)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)50μl，待(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)先加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)稀(xi)釋(shi)液40μl，然后(hou)(hou)(hou)再加(jia)(jia)(jia)(jia)(jia)(jia)待(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)終(zhong)稀(xi)釋(shi)度為5倍(bei)）。加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)于(yu)(yu)酶標(biao)(biao)(biao)(biao)(biao)板(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底(di)部，盡量(liang)(liang)不(bu)觸及(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)輕(qing)晃動混勻(yun)(yun)。3.溫(wen)(wen)育：用(yong)(yong)封(feng)(feng)板(ban)膜封(feng)(feng)板(ban)后(hou)(hou)(hou)置(zhi)(zhi)37℃溫(wen)(wen)育30分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。 4.配液：將(jiang)(jiang)(jiang)30倍(bei)濃(nong)縮洗(xi)(xi)滌(di)(di)(di)液用(yong)(yong)蒸餾水(shui)30倍(bei)稀(xi)釋(shi)后(hou)(hou)(hou)備(bei)用(yong)(yong)5.洗(xi)(xi)滌(di)(di)(di)：小(xiao)心(xin)揭掉封(feng)(feng)板(ban)膜，棄(qi)去(qu)(qu)液體(ti)(ti)(ti)(ti)，甩(shuai)干(gan)，每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)滿洗(xi)(xi)滌(di)(di)(di)液，靜置(zhi)(zhi)30后(hou)(hou)(hou)棄(qi)去(qu)(qu)，如此(ci)重(zhong)復(fu)5次，拍干(gan)。6.加(jia)(jia)(jia)(jia)(jia)(jia)酶：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)酶標(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)劑(ji)(ji)50μl，空(kong)白(bai)(bai)(bai)(bai)(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外(wai)(wai)。 7.溫(wen)(wen)育：操作(zuo)(zuo)同(tong)(tong)3。8.洗(xi)(xi)滌(di)(di)(di)：操作(zuo)(zuo)同(tong)(tong)5。9.顯(xian)色(se)(se)：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)顯(xian)色(se)(se)劑(ji)(ji)A50μl，再加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)顯(xian)色(se)(se)劑(ji)(ji)B50μl，輕(qing)輕(qing)震(zhen)蕩混勻(yun)(yun)，37℃避(bi)(bi)光(guang)顯(xian)色(se)(se)10分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong).10.終(zhong)止：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止液50μl，終(zhong)止反(fan)應(ying)(ying)（此(ci)時藍(lan)色(se)(se)立(li)轉黃色(se)(se)）。11.測(ce)(ce)(ce)(ce)定(ding)(ding)：以(yi)空(kong)白(bai)(bai)(bai)(bai)(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)調(diao)零，450nm波(bo)長(chang)依序測(ce)(ce)(ce)(ce)量(liang)(liang)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)吸光(guang)度（OD值）。測(ce)(ce)(ce)(ce)定(ding)(ding)應(ying)(ying)在加(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止液后(hou)(hou)(hou)15分(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)以(yi)內(nei)(nei)進(jin)(jin)行(xing)(xing)。牛(niu)雙(shuang)載(zai)(zai)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(AMPH) ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)盒 牛(niu)雙(shuang)載(zai)(zai)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(AMPH) ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)盒[售(shou)(shou)(shou)后(hou)(hou)(hou)]試(shi)(shi)(shi)(shi)劑(ji)(ji)盒售(shou)(shou)(shou)后(hou)(hou)(hou)：本(ben)公司出售(shou)(shou)(shou)的(de)(de)(de)試(shi)(shi)(shi)(shi)劑(ji)(ji)盒均(jun)(jun)保(bao)質保(bao)量(liang)(liang)，質量(liang)(liang)問題均(jun)(jun)可免費退(tui)換，另提(ti)供(gong)免費代測(ce)(ce)(ce)(ce)服務。細胞(bao)(bao)售(shou)(shou)(shou)后(hou)(hou)(hou)：1、細胞(bao)(bao)運輸(shu)丟失、瓶身破損(sun)、培養(yang)液嚴(yan)重(zhong)漏液等，重(zhong)發(fa)(fa)；2、細胞(bao)(bao)收到當(dang)天(tian)(tian)以(yi)及(ji)第2,3天(tian)(tian)請拍照，未告知的(de)(de)(de)視為產品(pin)(pin)合格。4-10天(tian)(tian)內(nei)(nei)出現問題，請提(ti)供(gong)細胞(bao)(bao)照片和(he)細胞(bao)(bao)出現問題的(de)(de)(de)照片以(yi)及(ji)細胞(bao)(bao)相關操作(zuo)(zuo)的(de)(de)(de)詳細步驟，并(bing)跟(gen)我公司人員及(ji)時溝通(tong)判定(ding)(ding)是否重(zhong)發(fa)(fa)，具體(ti)(ti)(ti)(ti)可以(yi)參照我官網(wang)或(huo)者(zhe)隨貨(huo)說(shuo)明(ming)書相關售(shou)(shou)(shou)后(hou)(hou)(hou)條(tiao)款。牛(niu)雙(shuang)載(zai)(zai)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(AMPH) ELISA 試(shi)(shi)(shi)(shi)劑(ji)(ji)盒
僅供研(yan)究(jiu)使用(yong)(yong)(yong)(yong) 牛γ氨基丁酸(GABA) ELISA 試(shi)(shi)劑(ji)盒實(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)原(yuan)(yuan)理： 試(shi)(shi)劑(ji)盒采用(yong)(yong)(yong)(yong)雙抗(kang)(kang)體(ti)(ti)(ti)(ti)一(yi)步(bu)夾心(xin)(xin)法酶(mei)聯(lian)免(mian)疫吸附試(shi)(shi)驗(yan)(yan)（ELISA）。往預(yu)先(xian)包被(bei)人(ren)（Human）抗(kang)(kang)AKAP3抗(kang)(kang)體(ti)(ti)(ti)(ti)（Anti-AKAP3 Ab）捕獲(huo)抗(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被(bei)微(wei)孔(kong)(kong)(kong)中(zhong)(zhong)，依次(ci)加(jia)(jia)入(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)測抗(kang)(kang)體(ti)(ti)(ti)(ti)，經過溫(wen)育并徹底(di)(di)洗滌。用(yong)(yong)(yong)(yong)底(di)(di)物(wu)(wu)TMB顯(xian)(xian)色(se)，TMB在過氧化(hua)物(wu)(wu)酶(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催(cui)化(hua)下(xia)轉(zhuan)化(hua)成藍(lan)(lan)色(se)，并在酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)下(xia)轉(zhuan)化(hua)成＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)。顏色(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)樣(yang)(yang)品(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)人(ren)（Human）抗(kang)(kang)AKAP3抗(kang)(kang)體(ti)(ti)(ti)(ti)（Anti-AKAP3Ab）呈正相關。用(yong)(yong)(yong)(yong)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在450nm 波(bo)長下(xia)測定(ding)(ding)吸光度(du)(du)（OD 值(zhi)(zhi)），計(ji)(ji)算(suan)樣(yang)(yang)品(pin)(pin)(pin)濃(nong)度(du)(du)。試(shi)(shi)劑(ji)盒組(zu)成：試(shi)(shi)劑(ji)盒組(zu)成48孔(kong)(kong)(kong)配(pei)(pei)(pei)置96孔(kong)(kong)(kong)配(pei)(pei)(pei)置保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)說明(ming)書1份(fen)1份(fen)密封(feng)(feng)袋(dai)1個1個封(feng)(feng)板(ban)(ban)(ban)膜12孔(kong)(kong)(kong)×4條2片（96）2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)微(wei)孔(kong)(kong)(kong)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)1×4812孔(kong)(kong)(kong)×8條2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)劑(ji)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)樣(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)顯(xian)(xian)色(se)劑(ji)A液(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)顯(xian)(xian)色(se)劑(ji)B液(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)終(zhong)止液(ye)(ye)3ml×1瓶(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)注意：使用(yong)(yong)(yong)(yong)前(qian)(qian)(qian)請(qing)檢(jian)(jian)查(cha)試(shi)(shi)劑(ji)盒中(zhong)(zhong)試(shi)(shi)劑(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽(qian)和(he)數(shu)(shu)(shu)量(liang)(liang)與(yu)表格是(shi)否一(yi)致(zhi)(zhi)。試(shi)(shi)劑(ji)盒操作(zuo)(zuo)(zuo)(zuo)步(bu)驟(zou)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采集及保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)：血清：全血標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)請(qing)于(yu)室溫(wen)放(fang)置2小(xiao)(xiao)時(shi)或(huo)(huo)4℃過一(yi)晚后(hou)(hou)于(yu)1000 x g離(li)心(xin)(xin)20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，取上(shang)(shang)清即(ji)(ji)可(ke)檢(jian)(jian)測，或(huo)(huo)將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)于(yu)-20℃或(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)，但應(ying)避(bi)免(mian)反(fan)復凍融(rong)。血漿：可(ke)用(yong)(yong)(yong)(yong)EDTA或(huo)(huo)肝素(su)(su)作(zuo)(zuo)(zuo)(zuo)為抗(kang)(kang)凝劑(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)采集后(hou)(hou)30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)于(yu)2 - 8° C 1000 x g離(li)心(xin)(xin)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，或(huo)(huo)將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)于(yu)-20℃或(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)，但應(ying)避(bi)免(mian)反(fan)復凍融(rong)。細胞培(pei)養(yang)物(wu)(wu)上(shang)(shang)清或(huo)(huo)其(qi)它生(sheng)(sheng)物(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)：1000 x g離(li)心(xin)(xin)20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，取上(shang)(shang)清即(ji)(ji)可(ke)檢(jian)(jian)測，或(huo)(huo)將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)于(yu)-20℃或(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)，但應(ying)避(bi)免(mian)反(fan)復凍融(rong)。 (注：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)溶(rong)血會影響**檢(jian)(jian)測結(jie)果(guo)(guo)，因此(ci)溶(rong)血標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)不(bu)宜進行此(ci)項檢(jian)(jian)測。)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)原(yuan)(yuan)則(ze)：首先(xian)通過文獻檢(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)式了(le)(le)解(jie)待測樣(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致(zhi)(zhi)含(han)(han)量(liang)(liang)，確(que)定(ding)(ding)適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)。只有稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍內(nei)，檢(jian)(jian)測的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果(guo)(guo)才是(shi)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過程(cheng)中(zhong)(zhong)，應(ying)做好(hao)詳細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)錄。計(ji)(ji)算(suan)濃(nong)度(du)(du)時(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)了(le)(le)“N”倍(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)應(ying)再乘(cheng)以(yi)(yi)(yi)“N”。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)原(yuan)(yuan)則(ze)：2瓶(ping)(ping)(ping)，每(mei)(mei)瓶(ping)(ping)(ping)臨用(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)樣(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)至1ml，蓋好(hao)后(hou)(hou)靜置10分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)上(shang)(shang)，然后(hou)(hou)反(fan)復顛倒(dao)/搓(cuo)動以(yi)(yi)(yi)助溶(rong)解(jie)，其(qi)濃(nong)度(du)(du)為300 pg/ml，做系列倍(bei)比稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)，分(fen)(fen)(fen)(fen)別稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)直接作(zuo)(zuo)(zuo)(zuo)為標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)度(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)前(qian)(qian)(qian)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。 如(ru)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)：取0.5ml（不(bu)要少于(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)加(jia)(jia)入(ru)(ru)含(han)(han)0.5ml樣(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中(zhong)(zhong)，混(hun)勻(yun)即(ji)(ji)可(ke)，其(qi)余濃(nong)度(du)(du)以(yi)(yi)(yi)此(ci)類推。生(sheng)(sheng)物(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)原(yuan)(yuan)則(ze)：臨用(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)生(sheng)(sheng)物(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)據預(yu)先(xian)計(ji)(ji)算(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)次(ci)實(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)孔(kong)(kong)(kong)100μl），實(shi)(shi)(shi)(shi)(shi)際配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)應(ying)多配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)10μl生(sheng)(sheng)物(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)990μl生(sheng)(sheng)物(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)勻(yun)，在使用(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)小(xiao)(xiao)時(shi)內(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。辣(la)根(gen)過氧化(hua)物(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)親和(he)素(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)原(yuan)(yuan)則(ze)：臨用(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)辣(la)根(gen)過氧化(hua)物(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)親和(he)素(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)據預(yu)先(xian)計(ji)(ji)算(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)次(ci)實(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)孔(kong)(kong)(kong)100μl），實(shi)(shi)(shi)(shi)(shi)際配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)應(ying)多配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)10μl辣(la)根(gen)過氧化(hua)物(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)親和(he)素(su)(su)加(jia)(jia)990μl辣(la)根(gen)過氧化(hua)物(wu)(wu)酶(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)親和(he)素(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)勻(yun)，在使用(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)小(xiao)(xiao)時(shi)內(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)實(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)結(jie)果(guo)(guo)計(ji)(ji)算(suan)： 以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)為橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)為縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在半對數(shu)(shu)(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線，根(gen)據樣(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線查(cha)出(chu)相應(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)；再乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)；或(huo)(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)與(yu)OD值(zhi)(zhi)計(ji)(ji)算(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線回歸(gui)方(fang)程(cheng)式，將樣(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)代入(ru)(ru)方(fang)程(cheng)式，計(ji)(ji)算(suan)出(chu)樣(yang)(yang)品(pin)(pin)(pin)濃(nong)度(du)(du)，再乘(cheng)以(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)，即(ji)(ji)為樣(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)(shi)(shi)際濃(nong)度(du)(du)。繪制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線：在Excel工作(zuo)(zuo)(zuo)(zuo)表中(zhong)(zhong)，以(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)濃(nong)度(du)(du)作(zuo)(zuo)(zuo)(zuo)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)，對應(ying)OD值(zhi)(zhi)作(zuo)(zuo)(zuo)(zuo)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)，繪制(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)線性回歸(gui)曲線，按(an)曲線方(fang)程(cheng)計(ji)(ji)算(suan)各樣(yang)(yang)本(ben)(ben)(ben)(ben)濃(nong)度(du)(du)值(zhi)(zhi)。牛γ氨基丁酸(GABA) ELISA 試(shi)(shi)劑(ji)盒 技術小(xiao)(xiao)提(ti)示：1. 當混(hun)合或(huo)(huo)重溶(rong)蛋白溶(rong)液(ye)(ye)時(shi)，盡(jin)量(liang)(liang)避(bi)免(mian)起沫。2. 為了(le)(le)避(bi)免(mian)交叉感染，配(pei)(pei)(pei)置不(bu)同濃(nong)度(du)(du)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)、上(shang)(shang)樣(yang)(yang)、加(jia)(jia)不(bu)同試(shi)(shi)劑(ji)都需要更換槍(qiang)頭(tou)。另外不(bu)同試(shi)(shi)劑(ji)請(qing)分(fen)(fen)(fen)(fen)別使用(yong)(yong)(yong)(yong)不(bu)同的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)移液(ye)(ye)槽。3. 每(mei)(mei)次(ci)孵育時(shi)，請(qing)正確(que)使用(yong)(yong)(yong)(yong)封(feng)(feng)板(ban)(ban)(ban)膠可(ke)保(bao)(bao)(bao)(bao)(bao)證結(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性。4. 混(hun)合后(hou)(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)顯(xian)(xian)色(se)底(di)(di)物(wu)(wu)在上(shang)(shang)板(ban)(ban)(ban)前(qian)(qian)(qian)應(ying)為無色(se)，請(qing)避(bi)光保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)；假如(ru)微(wei)孔(kong)(kong)(kong)板(ban)(ban)(ban)后(hou)(hou)，將由物(wu)(wu)色(se)變成不(bu)同深度(du)(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)藍(lan)(lan)色(se)。5. 終(zhong)止液(ye)(ye)上(shang)(shang)板(ban)(ban)(ban)順序(xu)應(ying)同顯(xian)(xian)色(se)底(di)(di)物(wu)(wu)上(shang)(shang)板(ban)(ban)(ban)順序(xu)一(yi)致(zhi)(zhi)；加(jia)(jia)入(ru)(ru)終(zhong)止液(ye)(ye)后(hou)(hou)，孔(kong)(kong)(kong)內(nei)顏色(se)由藍(lan)(lan)變黃；若(ruo)孔(kong)(kong)(kong)內(nei)有綠色(se)，則(ze)表明(ming)孔(kong)(kong)(kong)內(nei)液(ye)(ye)體(ti)(ti)(ti)(ti)未混(hun)勻(yun)；請(qing)充分(fen)(fen)(fen)(fen)混(hun)合。產品(pin)(pin)(pin)規格：96T/48T（可(ke)拆）產品(pin)(pin)(pin)應(ying)用(yong)(yong)(yong)(yong)：ELISA實(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)貨(huo)期：3-5天有效期：6個月保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)條件(jian)：2-8℃
僅供研(yan)究使(shi)(shi)(shi)用(yong)(yong)(yong)(yong) 牛(niu)X-框結(jie)(jie)(jie)合(he)蛋白(bai)(bai)(bai)(bai)1(XBP1)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)實(shi)驗原(yuan)理： 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)采(cai)用(yong)(yong)(yong)(yong)雙(shuang)抗(kang)體(ti)(ti)(ti)(ti)一(yi)步(bu)夾心(xin)(xin)法酶(mei)(mei)(mei)(mei)(mei)聯免疫吸附試(shi)(shi)(shi)(shi)(shi)驗（ELISA）。往(wang)預先包被牛(niu)X-框結(jie)(jie)(jie)合(he)蛋白(bai)(bai)(bai)(bai)1(XBP1)捕獲(huo)抗(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，依次(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)體(ti)(ti)(ti)(ti)，經(jing)過(guo)(guo)(guo)(guo)溫(wen)育(yu)并徹底洗滌(di)。用(yong)(yong)(yong)(yong)底物(wu)(wu)(wu)(wu)TMB顯色(se)(se)，TMB在(zai)(zai)過(guo)(guo)(guo)(guo)氧(yang)化(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)下(xia)轉(zhuan)化(hua)成(cheng)藍色(se)(se)，并在(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)下(xia)轉(zhuan)化(hua)成(cheng)＊終(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)(se)。顏色(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)深淺(qian)和(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)牛(niu)X-框結(jie)(jie)(jie)合(he)蛋白(bai)(bai)(bai)(bai)1(XBP1)呈(cheng)正相(xiang)關。用(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)450nm 波(bo)長下(xia)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定吸光度(du)(du)(du)(du)(du)(du)（OD 值），計(ji)算樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)。試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)組(zu)成(cheng)：試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)組(zu)成(cheng)48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)(zhi)96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)(zhi)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)說(shuo)明(ming)書1份1份密封袋1個1個封板(ban)(ban)(ban)膜12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)2片（96）2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)1.5ml×1瓶(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)顯色(se)(se)劑(ji)(ji)A液(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)顯色(se)(se)劑(ji)(ji)B液(ye)(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)6 ml×1瓶(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)終(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)3ml×1瓶(ping)6ml×1瓶(ping)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)注意(yi)：使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)請(qing)(qing)(qing)檢(jian)查(cha)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)中(zhong)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和(he)(he)(he)數(shu)(shu)(shu)量(liang)(liang)與表格是否一(yi)致。試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)操作(zuo)(zuo)步(bu)驟(zou)： 實(shi)驗開(kai)始前(qian)(qian)(qian)(qian)，請(qing)(qing)(qing)提前(qian)(qian)(qian)(qian)配(pei)置(zhi)(zhi)好(hao)(hao)所有試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)或(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)，均需混(hun)(hun)(hun)勻(yun)(yun)(yun)，混(hun)(hun)(hun)勻(yun)(yun)(yun)時(shi)(shi)盡(jin)(jin)量(liang)(liang)避(bi)免起泡(pao)(pao)(pao)。每(mei)次(ci)檢(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)(ying)(ying)(ying)該(gai)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)。如(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)過(guo)(guo)(guo)(guo)高時(shi)(shi)，用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)進行稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)(yi)使(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)符合(he)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)范圍。1.加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)別設空白(bai)(bai)(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。空白(bai)(bai)(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)100μl，注意(yi)不(bu)(bu)(bu)要有氣泡(pao)(pao)(pao)，加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)(yu)(yu)(yu)(yu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部，盡(jin)(jin)量(liang)(liang)不(bu)(bu)(bu)觸(chu)及(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)(qing)(qing)(qing)輕(qing)(qing)(qing)(qing)晃動(dong)混(hun)(hun)(hun)勻(yun)(yun)(yun)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)(shang)(shang)蓋(gai)或(huo)(huo)(huo)覆膜，37℃反(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。 為(wei)保(bao)(bao)(bao)(bao)(bao)證實(shi)驗結(jie)(jie)(jie)果(guo)有效(xiao)性(xing)(xing)(xing)，每(mei)次(ci)實(shi)驗請(qing)(qing)(qing)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)。2.棄去(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩干，不(bu)(bu)(bu)用(yong)(yong)(yong)(yong)洗滌(di)。每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)制(zhi)(zhi)，輕(qing)(qing)(qing)(qing)輕(qing)(qing)(qing)(qing)混(hun)(hun)(hun)勻(yun)(yun)(yun)，在(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一(yi)小(xiao)時(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)制(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。3.溫(wen)育(yu)60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)(hou)(hou)，棄去(qu)(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩干，洗板(ban)(ban)(ban)3次(ci)，每(mei)次(ci)浸(jin)泡(pao)(pao)(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，350μl/每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩干。4.每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)辣根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)化(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)（同(tong)(tong)生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)。5.溫(wen)育(yu)60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)后(hou)(hou)(hou)(hou)，棄去(qu)(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩干，洗板(ban)(ban)(ban)5次(ci)，每(mei)次(ci)浸(jin)泡(pao)(pao)(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，350μl/每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩干。6.依序每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)底物(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)光顯色(se)(se)（30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)，此(ci)(ci)時(shi)(shi)肉眼可(ke)見(jian)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)有明(ming)顯的(de)(de)(de)(de)(de)(de)(de)(de)梯(ti)度(du)(du)(du)(du)(du)(du)藍色(se)(se)，后(hou)(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)梯(ti)度(du)(du)(du)(du)(du)(du)不(bu)(bu)(bu)明(ming)顯，即(ji)可(ke)終(zhong)(zhong)止）。7.依序每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)止溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)止反(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)（此(ci)(ci)時(shi)(shi)藍色(se)(se)立(li)轉(zhuan)黃色(se)(se)）。終(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)順序應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)(jin)量(liang)(liang)與底物(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)順序相(xiang)同(tong)(tong)。為(wei)了保(bao)(bao)(bao)(bao)(bao)證實(shi)驗結(jie)(jie)(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)確性(xing)(xing)(xing)，底物(wu)(wu)(wu)(wu)反(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)時(shi)(shi)間到后(hou)(hou)(hou)(hou)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)(jin)快加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)終(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)聯儀在(zai)(zai)450nm波(bo)長依序測(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)光密度(du)(du)(du)(du)(du)(du)（OD值）。在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)止液(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)內(nei)(nei)(nei)(nei)(nei)進行檢(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)的(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集及(ji)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)：血(xue)(xue)清(qing)：全血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)請(qing)(qing)(qing)于(yu)(yu)(yu)(yu)(yu)室溫(wen)放(fang)置(zhi)(zhi)2小(xiao)時(shi)(shi)或(huo)(huo)(huo)4℃過(guo)(guo)(guo)(guo)一(yi)晚后(hou)(hou)(hou)(hou)于(yu)(yu)(yu)(yu)(yu)1000 x g離心(xin)(xin)20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，取上(shang)(shang)(shang)清(qing)即(ji)可(ke)檢(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)放(fang)于(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)免反(fan)(fan)(fan)(fan)(fan)復(fu)(fu)凍融。血(xue)(xue)漿：可(ke)用(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)肝素(su)(su)作(zuo)(zuo)為(wei)抗(kang)凝劑(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)采(cai)集后(hou)(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)于(yu)(yu)(yu)(yu)(yu)2 - 8° C 1000 x g離心(xin)(xin)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，或(huo)(huo)(huo)將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)放(fang)于(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)免反(fan)(fan)(fan)(fan)(fan)復(fu)(fu)凍融。細胞培(pei)養(yang)物(wu)(wu)(wu)(wu)上(shang)(shang)(shang)清(qing)或(huo)(huo)(huo)其(qi)它生(sheng)(sheng)物(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)：1000 x g離心(xin)(xin)20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)，取上(shang)(shang)(shang)清(qing)即(ji)可(ke)檢(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)放(fang)于(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)，但(dan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)免反(fan)(fan)(fan)(fan)(fan)復(fu)(fu)凍融。 (注：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)溶(rong)(rong)(rong)血(xue)(xue)會影響**檢(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)(jie)果(guo)，因此(ci)(ci)溶(rong)(rong)(rong)血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)不(bu)(bu)(bu)宜進行此(ci)(ci)項(xiang)檢(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)。)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)：首先通(tong)過(guo)(guo)(guo)(guo)文獻檢(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)方式了解待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)的(de)(de)(de)(de)(de)(de)(de)(de)大(da)致含(han)量(liang)(liang)，確定適當的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)。只(zhi)有稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)范圍內(nei)(nei)(nei)(nei)(nei)，檢(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)結(jie)(jie)(jie)果(guo)才(cai)是準(zhun)(zhun)(zhun)確的(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)程中(zhong)，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)做好(hao)(hao)詳細的(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)(ji)(ji)錄。計(ji)算濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)時(shi)(shi)，稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)再(zai)(zai)(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)“N”。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)：2瓶(ping)，每(mei)瓶(ping)臨用(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)至1ml，蓋(gai)好(hao)(hao)后(hou)(hou)(hou)(hou)靜置(zhi)(zhi)10分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)上(shang)(shang)(shang)，然后(hou)(hou)(hou)(hou)反(fan)(fan)(fan)(fan)(fan)復(fu)(fu)顛倒/搓(cuo)動(dong)以(yi)(yi)(yi)(yi)(yi)助溶(rong)(rong)(rong)解，其(qi)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)300 pg/ml，做系(xi)列(lie)倍(bei)(bei)比(bi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)別稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)直接(jie)作(zuo)(zuo)為(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)配(pei)制(zhi)(zhi)。 如(ru)(ru)配(pei)制(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)：取0.5ml（不(bu)(bu)(bu)要少(shao)于(yu)(yu)(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)述(shu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)含(han)0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中(zhong)，混(hun)(hun)(hun)勻(yun)(yun)(yun)即(ji)可(ke)，其(qi)余濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)以(yi)(yi)(yi)(yi)(yi)此(ci)(ci)類推。生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)：臨用(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)根(gen)(gen)據(ju)預先計(ji)算好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)每(mei)次(ci)實(shi)驗所需的(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)配(pei)制(zhi)(zhi)（每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際配(pei)制(zhi)(zhi)時(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)多配(pei)制(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)制(zhi)(zhi)，輕(qing)(qing)(qing)(qing)輕(qing)(qing)(qing)(qing)混(hun)(hun)(hun)勻(yun)(yun)(yun)，在(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一(yi)小(xiao)時(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)制(zhi)(zhi)。辣根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)化(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)(ze)：臨用(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)辣根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)化(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)(qian)根(gen)(gen)據(ju)預先計(ji)算好(hao)(hao)的(de)(de)(de)(de)(de)(de)(de)(de)每(mei)次(ci)實(shi)驗所需的(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)(liang)配(pei)制(zhi)(zhi)（每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際配(pei)制(zhi)(zhi)時(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)多配(pei)制(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl辣根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)化(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl辣根(gen)(gen)過(guo)(guo)(guo)(guo)氧(yang)化(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親(qin)(qin)(qin)和(he)(he)(he)素(su)(su)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)制(zhi)(zhi)，輕(qing)(qing)(qing)(qing)輕(qing)(qing)(qing)(qing)混(hun)(hun)(hun)勻(yun)(yun)(yun)，在(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前(qian)(qian)(qian)(qian)一(yi)小(xiao)時(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)制(zhi)(zhi)實(shi)驗結(jie)(jie)(jie)果(guo)計(ji)算： 以(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)橫坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對(dui)數(shu)(shu)(shu)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值為(wei)縱(zong)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通(tong)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)半對(dui)數(shu)(shu)(shu)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)繪(hui)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，根(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)OD值由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)查(cha)出(chu)相(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)；再(zai)(zai)(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)與OD值計(ji)算出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)回(hui)歸方程式，將樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)OD值代入(ru)(ru)方程式，計(ji)算出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)，再(zai)(zai)(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)，即(ji)為(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)。繪(hui)制(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)：在(zai)(zai)Excel工(gong)(gong)作(zuo)(zuo)表中(zhong)，以(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)作(zuo)(zuo)橫坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，對(dui)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)OD值作(zuo)(zuo)縱(zong)坐(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，繪(hui)制(zhi)(zhi)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)(xian)性(xing)(xing)(xing)回(hui)歸曲(qu)線(xian)(xian)(xian)，按曲(qu)線(xian)(xian)(xian)方程計(ji)算各樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)值。注意(yi)事(shi)項(xiang)：1.當混(hun)(hun)(hun)合(he)蛋白(bai)(bai)(bai)(bai)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)(jin)量(liang)(liang)輕(qing)(qing)(qing)(qing)緩(huan)，避(bi)免起泡(pao)(pao)(pao)。2.洗滌(di)過(guo)(guo)(guo)(guo)程非常重要，不(bu)(bu)(bu)充分(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)洗滌(di)易造成(cheng)假陽性(xing)(xing)(xing)。3.一(yi)次(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)間**控制(zhi)(zhi)在(zai)(zai)5分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)數(shu)(shu)(shu)量(liang)(liang)多，建議使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4.請(qing)(qing)(qing)每(mei)次(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定的(de)(de)(de)(de)(de)(de)(de)(de)同(tong)(tong)時(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，做復(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。5.如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)中(zhong)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)質含(han)量(liang)(liang)過(guo)(guo)(guo)(guo)高，請(qing)(qing)(qing)先稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)再(zai)(zai)(zai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定，計(ji)算時(shi)(shi)請(qing)(qing)(qing)乘(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)(shu)。6.在(zai)(zai)配(pei)制(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)，請(qing)(qing)(qing)以(yi)(yi)(yi)(yi)(yi)相(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)配(pei)制(zhi)(zhi)，不(bu)(bu)(bu)能混(hun)(hun)(hun)淆(xiao)。7.底物(wu)(wu)(wu)(wu)請(qing)(qing)(qing)避(bi)光保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)。8.不(bu)(bu)(bu)要用(yong)(yong)(yong)(yong)其(qi)它生(sheng)(sheng)產廠(chang)家的(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)替換試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)。牛(niu)X-框結(jie)(jie)(jie)合(he)蛋白(bai)(bai)(bai)(bai)1(XBP1)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)性(xing)(xing)(xing)能1．準(zhun)(zhun)(zhun)確性(xing)(xing)(xing)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)(xian)性(xing)(xing)(xing)回(hui)歸與預期濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)相(xiang)關系(xi)數(shu)(shu)(shu)R值，大(da)于(yu)(yu)(yu)(yu)(yu)等(deng)于(yu)(yu)(yu)(yu)(yu)0.9900。2．靈敏度(du)(du)(du)(du)(du)(du)：＊低檢(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)(du)小(xiao)于(yu)(yu)(yu)(yu)(yu)10 pg/ml。3．特異(yi)性(xing)(xing)(xing)：不(bu)(bu)(bu)與其(qi)它可(ke)溶(rong)(rong)(rong)性(xing)(xing)(xing)結(jie)(jie)(jie)構類似(si)物(wu)(wu)(wu)(wu)交(jiao)叉反(fan)(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)。4．重復(fu)(fu)性(xing)(xing)(xing)：批(pi)內(nei)(nei)(nei)(nei)(nei)與批(pi)見(jian)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)別小(xiao)于(yu)(yu)(yu)(yu)(yu)9%和(he)(he)(he)15%。
牛(niu)角蛋白(bai)1(KRT1) ELISA 試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)文名稱(cheng)牛(niu)抗(kang)神經(jing)元核抗(kang)體(ti)2型(xing)/抗(kang)Ri抗(kang)體(ti)(ANNA-2/Ri) ELISA 試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)規格(ge)96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)類型(xing)血(xue)清、血(xue)漿(jiang)、細胞(bao)上(shang)清液(ye)(ye)(ye)(ye)(ye)、尿(niao)液(ye)(ye)(ye)(ye)(ye)、體(ti)液(ye)(ye)(ye)(ye)(ye)、灌洗(xi)液(ye)(ye)(ye)(ye)(ye)等使(shi)(shi)(shi)用(yong)(yong)范(fan)圍科研實(shi)驗，不(bu)得(de)用(yong)(yong)于(yu)(yu)臨床診斷(duan)反應(ying)時(shi)間1-5h試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)保(bao)(bao)存(cun)(cun)(cun)(cun)2-8℃有(you)(you)效(xiao)期(qi)6個(ge)月貨期(qi)3-5天【操(cao)作(zuo)步驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)與(yu)(yu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)上(shang)設標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)100μl，然后(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)；然后(hou)(hou)(hou)從(cong)(cong)第(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)(di)(di)二(er)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)各取100μl分(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)(dao)(dao)第(di)(di)(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)(he)第(di)(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)三(san)、第(di)(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)；然后(hou)(hou)(hou)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)三(san)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)(he)第(di)(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)先(xian)各取50μl棄(qi)掉(diao)，再(zai)(zai)(zai)各取50μl分(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)(dao)(dao)第(di)(di)(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)(di)(di)六(liu)(liu)(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，再(zai)(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)(di)(di)六(liu)(liu)(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50ul，混(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)；混(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)后(hou)(hou)(hou)從(cong)(cong)第(di)(di)(di)(di)(di)(di)(di)(di)五、第(di)(di)(di)(di)(di)(di)(di)(di)六(liu)(liu)(liu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)各取50μl分(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)(dao)(dao)第(di)(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，再(zai)(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)后(hou)(hou)(hou)從(cong)(cong)第(di)(di)(di)(di)(di)(di)(di)(di)七、第(di)(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)分(fen)(fen)(fen)(fen)別(bie)(bie)取50μl加(jia)(jia)(jia)(jia)(jia)(jia)(jia)到(dao)(dao)(dao)第(di)(di)(di)(di)(di)(di)(di)(di)九(jiu)、第(di)(di)(di)(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，再(zai)(zai)(zai)在(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別(bie)(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)后(hou)(hou)(hou)從(cong)(cong)第(di)(di)(di)(di)(di)(di)(di)(di)九(jiu)第(di)(di)(di)(di)(di)(di)(di)(di)十(shi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)各取50μl棄(qi)掉(diao)。（稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)(hou)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)都為(wei)(wei)50μl，濃(nong)(nong)度(du)(du)(du)分(fen)(fen)(fen)(fen)別(bie)(bie)為(wei)(wei)3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)別(bie)(bie)設空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)白(bai)對(dui)照孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)及(ji)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，其(qi)(qi)(qi)(qi)余各步操(cao)作(zuo)相(xiang)同）、待(dai)(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)上(shang)待(dai)(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)40μl，然后(hou)(hou)(hou)再(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)待(dai)(dai)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)＊終(zhong)(zhong)(zhong)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)度(du)(du)(du)為(wei)(wei)5倍(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)(yu)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部(bu)，盡量(liang)(liang)不(bu)觸及(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)輕(qing)晃動混(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)。3. 溫(wen)育(yu)(yu)：用(yong)(yong)封(feng)板(ban)(ban)膜封(feng)板(ban)(ban)后(hou)(hou)(hou)置37℃溫(wen)育(yu)(yu)30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。4. 配液(ye)(ye)(ye)(ye)(ye)：將(jiang)30（48T的(de)(de)(de)20倍(bei)(bei)）倍(bei)(bei)濃(nong)(nong)縮洗(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)用(yong)(yong)蒸餾水(shui)30（48T的(de)(de)(de)20倍(bei)(bei)）倍(bei)(bei)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)(hou)備用(yong)(yong)。5. 洗(xi)滌(di)：小(xiao)心(xin)揭掉(diao)封(feng)板(ban)(ban)膜，棄(qi)去(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)，甩干，每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)滿洗(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)，靜置30秒后(hou)(hou)(hou)棄(qi)去(qu)，如此(ci)重(zhong)復5次(ci)，拍(pai)干。6. 加(jia)(jia)(jia)(jia)(jia)(jia)(jia)酶：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)50μl，空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外。7. 溫(wen)育(yu)(yu)：操(cao)作(zuo)同3。8. 洗(xi)滌(di)：操(cao)作(zuo)同5。9. 顯(xian)色(se)(se)：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)顯(xian)色(se)(se)劑(ji)(ji)(ji)(ji)(ji)A50μl，再(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)顯(xian)色(se)(se)劑(ji)(ji)(ji)(ji)(ji)B50μl，輕(qing)輕(qing)震蕩(dang)混(hun)(hun)(hun)勻(yun)(yun)(yun)(yun)，37℃避(bi)光(guang)顯(xian)色(se)(se)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong). 10. 終(zhong)(zhong)(zhong)止(zhi)：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)(zhong)止(zhi)反應(ying)（此(ci)時(shi)藍色(se)(se)立(li)轉黃色(se)(se)）。11. 測(ce)(ce)(ce)定(ding)(ding)(ding)：以空(kong)白(bai)空(kong)調零，450nm波長依序測(ce)(ce)(ce)量(liang)(liang)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)吸光(guang)度(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)定(ding)(ding)(ding)應(ying)在(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以內(nei)(nei)進(jin)行(xing)(xing)(xing)。牛(niu)抗(kang)神經(jing)元核抗(kang)體(ti)2型(xing)/抗(kang)Ri抗(kang)體(ti)(ANNA-2/Ri)ELISA 試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)【注意事項】1． 試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)從(cong)(cong)冷藏環境中(zhong)(zhong)取出應(ying)在(zai)(zai)室溫(wen)平衡(heng)15-30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)方可(ke)使(shi)(shi)(shi)用(yong)(yong)，酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)開封(feng)后(hou)(hou)(hou)如未用(yong)(yong)完(wan)，板(ban)(ban)條應(ying)裝入(ru)(ru)密封(feng)袋中(zhong)(zhong)保(bao)(bao)存(cun)(cun)(cun)(cun)。2． 濃(nong)(nong)洗(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)可(ke)能會有(you)(you)結(jie)(jie)晶析(xi)出，稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)時(shi)可(ke)在(zai)(zai)水(shui)浴中(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)溫(wen)助(zhu)溶，洗(xi)滌(di)時(shi)不(bu)影響結(jie)(jie)果(guo)。3． 各步加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)均(jun)(jun)應(ying)使(shi)(shi)(shi)用(yong)(yong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)器，并經(jing)常(chang)校對(dui)其(qi)(qi)(qi)(qi)準(zhun)(zhun)(zhun)(zhun)確(que)性，以避(bi)免(mian)試(shi)(shi)(shi)(shi)(shi)(shi)驗誤(wu)差(cha)(cha)(cha)。一(yi)(yi)次(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)間＊好控制在(zai)(zai)5分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)，如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)(shu)量(liang)(liang)多，推薦使(shi)(shi)(shi)用(yong)(yong)排(pai)槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4． 請(qing)每(mei)(mei)(mei)次(ci)測(ce)(ce)(ce)定(ding)(ding)(ding)的(de)(de)(de)同時(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，＊好做復孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中(zhong)(zhong)待(dai)(dai)測(ce)(ce)(ce)物(wu)(wu)(wu)質含量(liang)(liang)過高（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)OD值(zhi)(zhi)(zhi)(zhi)大于(yu)(yu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)），請(qing)先(xian)用(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)一(yi)(yi)定(ding)(ding)(ding)倍(bei)(bei)數(shu)(shu)（n倍(bei)(bei)）后(hou)(hou)(hou)再(zai)(zai)(zai)測(ce)(ce)(ce)定(ding)(ding)(ding)，計(ji)(ji)(ji)算(suan)(suan)(suan)時(shi)請(qing)＊后(hou)(hou)(hou)乘(cheng)以總稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)（×n×5）。5． 封(feng)板(ban)(ban)膜只(zhi)限(xian)一(yi)(yi)次(ci)性使(shi)(shi)(shi)用(yong)(yong)，以避(bi)免(mian)交叉(cha)污染。6． 底物(wu)(wu)(wu)請(qing)避(bi)光(guang)保(bao)(bao)存(cun)(cun)(cun)(cun)。7． 嚴格(ge)按(an)照說明(ming)(ming)書(shu)(shu)的(de)(de)(de)操(cao)作(zuo)進(jin)行(xing)(xing)(xing)，試(shi)(shi)(shi)(shi)(shi)(shi)驗結(jie)(jie)果(guo)判定(ding)(ding)(ding)必須以酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀讀數(shu)(shu)為(wei)(wei)準(zhun)(zhun)(zhun)(zhun).8． 所有(you)(you)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)，洗(xi)滌(di)液(ye)(ye)(ye)(ye)(ye)和(he)(he)(he)各種廢(fei)棄(qi)物(wu)(wu)(wu)都應(ying)按(an)傳染物(wu)(wu)(wu)處理。9． 本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)不(bu)同批(pi)號組(zu)(zu)分(fen)(fen)(fen)(fen)不(bu)得(de)混(hun)(hun)(hun)用(yong)(yong)。10. 如與(yu)(yu)英文說明(ming)(ming)書(shu)(shu)有(you)(you)異，以英文說明(ming)(ming)書(shu)(shu)為(wei)(wei)準(zhun)(zhun)(zhun)(zhun)。【計(ji)(ji)(ji)算(suan)(suan)(suan)】 以標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)(de)(de)濃(nong)(nong)度(du)(du)(du)為(wei)(wei)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)為(wei)(wei)縱(zong)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)， 在(zai)(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)繪出標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，根據樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)OD 值(zhi)(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)查(cha)出相(xiang)應(ying)的(de)(de)(de)濃(nong)(nong)度(du)(du)(du)；再(zai)(zai)(zai)乘(cheng)以稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)數(shu)(shu)；或(huo)用(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)(de)(de)濃(nong)(nong)度(du)(du)(du)與(yu)(yu)OD值(zhi)(zhi)(zhi)(zhi)計(ji)(ji)(ji)算(suan)(suan)(suan)出標(biao)(biao)(biao)(biao)(biao)(biao)(biao) 準(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)直線(xian)(xian)(xian)回(hui)歸方程(cheng)式，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi) 代(dai)入(ru)(ru)方程(cheng)式，計(ji)(ji)(ji)算(suan)(suan)(suan)出樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)，再(zai)(zai)(zai)乘(cheng)以稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi) 倍(bei)(bei)數(shu)(shu)，即為(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)實(shi)際濃(nong)(nong)度(du)(du)(du)。 【試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)組(zu)(zu)成】試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)組(zu)(zu)成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置保(bao)(bao)存(cun)(cun)(cun)(cun)說明(ming)(ming)書(shu)(shu)1份1份密封(feng)袋1個(ge)1個(ge)封(feng)板(ban)(ban)膜12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條2片（96）2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)酶標(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)顯(xian)色(se)(se)劑(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)顯(xian)色(se)(se)劑(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)終(zhong)(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)2-8℃保(bao)(bao)存(cun)(cun)(cun)(cun)注意：使(shi)(shi)(shi)用(yong)(yong)前請(qing)檢查(cha)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和(he)(he)(he)數(shu)(shu)量(liang)(liang)與(yu)(yu)表格(ge)是否一(yi)(yi)致。【精(jing)(jing)密度(du)(du)(du)】精(jing)(jing)密度(du)(du)(du)用(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)變異系數(shu)(shu)CV 表示。CV（%） = SD/mean×100批(pi)內(nei)(nei)差(cha)(cha)(cha)：取同批(pi)次(ci)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)對(dui)低、中(zhong)(zhong)、高值(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)進(jin)行(xing)(xing)(xing)定(ding)(ding)(ding)量(liang)(liang)檢測(ce)(ce)(ce)，每(mei)(mei)(mei)份樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)連(lian)續測(ce)(ce)(ce)定(ding)(ding)(ding)20 次(ci)，分(fen)(fen)(fen)(fen)別(bie)(bie)計(ji)(ji)(ji)算(suan)(suan)(suan)不(bu)同濃(nong)(nong)度(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)平均(jun)(jun)值(zhi)(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)(zhi)。批(pi)內(nei)(nei)差(cha)(cha)(cha)： CV<9%批(pi)間差(cha)(cha)(cha)：選取3 個(ge)不(bu)同批(pi)次(ci)的(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)分(fen)(fen)(fen)(fen)別(bie)(bie)對(dui)低、中(zhong)(zhong)、高值(zhi)(zhi)(zhi)(zhi)定(ding)(ding)(ding)值(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)進(jin)行(xing)(xing)(xing)定(ding)(ding)(ding)量(liang)(liang)測(ce)(ce)(ce)定(ding)(ding)(ding)，每(mei)(mei)(mei)個(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)使(shi)(shi)(shi)用(yong)(yong)同一(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)重(zhong)復測(ce)(ce)(ce)定(ding)(ding)(ding)10 次(ci)，分(fen)(fen)(fen)(fen)別(bie)(bie)計(ji)(ji)(ji)算(suan)(suan)(suan)不(bu)同濃(nong)(nong)度(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)平均(jun)(jun)值(zhi)(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)(zhi)。批(pi)間差(cha)(cha)(cha)： CV<11% 【特異性】本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)識別(bie)(bie)天然和(he)(he)(he)重(zhong)組(zu)(zu)微(wei)生物(wu)(wu)(wu)硝酸鹽還原(yuan)酶（Nar），經(jing)檢測(ce)(ce)(ce)與(yu)(yu)其(qi)(qi)(qi)(qi)它相(xiang)似(si)物(wu)(wu)(wu)質無(wu)明(ming)(ming)顯(xian)交叉(cha)反應(ying)。由于(yu)(yu)受到(dao)(dao)(dao)技術及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)來源的(de)(de)(de)限(xian)制，不(bu)可(ke)能完(wan)成所有(you)(you)相(xiang)關或(huo)相(xiang)似(si)物(wu)(wu)(wu)質交叉(cha)反應(ying)檢測(ce)(ce)(ce)，因此(ci)本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)有(you)(you)可(ke)能與(yu)(yu)未經(jing)檢測(ce)(ce)(ce)的(de)(de)(de)其(qi)(qi)(qi)(qi)它物(wu)(wu)(wu)質有(you)(you)交叉(cha)反應(ying)。 【穩(wen)定(ding)(ding)(ding)性】試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)在(zai)(zai)有(you)(you)效(xiao)期(qi)內(nei)(nei)務必按(an)推薦溫(wen)度(du)(du)(du)保(bao)(bao)存(cun)(cun)(cun)(cun)，活性降低率小(xiao)于(yu)(yu)5%。為(wei)(wei)減小(xiao)外部(bu)因素(su)對(dui)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)破壞(huai)前后(hou)(hou)(hou)檢測(ce)(ce)(ce)值(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)影響，實(shi)驗室的(de)(de)(de)環境條件需(xu)盡量(liang)(liang)保(bao)(bao)持一(yi)(yi)致，尤其(qi)(qi)(qi)(qi)是實(shi)驗室內(nei)(nei)溫(wen)度(du)(du)(du)、濕度(du)(du)(du)及(ji)溫(wen)育(yu)(yu)條件。其(qi)(qi)(qi)(qi)次(ci)由同一(yi)(yi)實(shi)驗員來進(jin)行(xing)(xing)(xing)操(cao)作(zuo)可(ke)減少人為(wei)(wei)誤(wu)差(cha)(cha)(cha)。
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