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                                 猴(hou)可溶性(xing)細胞間粘附(fu)分(fen)(fen)子1(sICAM-1)酶(mei)聯免(mian)(mian)疫試(shi)劑(ji)(ji)(ji)盒實(shi)(shi)驗原(yuan)(yuan)理    ELISA運用(yong)(yong)了免(mian)(mian)疫學原(yuan)(yuan)理和(he)(he)化學反應(ying)顯色原(yuan)(yuan)理，需(xu)要將(jiang)抗原(yuan)(yuan)或抗體(ti)(ti)預(yu)包被在(zai)(zai)(zai)(zai)(zai)固(gu)相(xiang)載體(ti)(ti)表面，使后(hou)來形成(cheng)的(de)(de)(de)(de)抗原(yuan)(yuan)-抗體(ti)(ti)-酶(mei) (熒光(guang)基團) -底(di)物復(fu)(fu)合(he)物黏附(fu)在(zai)(zai)(zai)(zai)(zai)載體(ti)(ti)上，通過檢(jian)(jian)測OD值(zhi)或發(fa)光(guang)值(zhi)，來檢(jian)(jian)測靶蛋(dan)白的(de)(de)(de)(de)含量。 實(shi)(shi)驗所(suo)需(xu)自(zi)(zi)備(bei)物品1. 酶(mei)標(biao)(biao)(biao)(biao)(biao)儀（450nm）2. 加(jia)(jia)樣(yang)(yang)(yang)(yang)器及槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒溫(wen)(wen)(wen)(wen)箱(xiang) 試(shi)劑(ji)(ji)(ji)盒組成(cheng)名稱96 孔(kong)(kong)(kong)配置(zhi)(zhi)48 孔(kong)(kong)(kong)配置(zhi)(zhi)備(bei)注微孔(kong)(kong)(kong)酶(mei)標(biao)(biao)(biao)(biao)(biao)板12 孔(kong)(kong)(kong)×8 條(tiao)12 孔(kong)(kong)(kong)×4 條(tiao)無(wu)(wu)標(biao)(biao)(biao)(biao)(biao)準(zhun)品0.5mL0.5mL按說(shuo)(shuo)明書進(jin)(jin)行(xing)(xing)稀(xi)(xi)釋(shi)標(biao)(biao)(biao)(biao)(biao)準(zhun)品稀(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)6mL3mL按說(shuo)(shuo)明書進(jin)(jin)行(xing)(xing)稀(xi)(xi)釋(shi)樣(yang)(yang)(yang)(yang)本(ben)稀(xi)(xi)釋(shi)液(ye)(ye)(ye)(ye)6mL3mL按說(shuo)(shuo)明書進(jin)(jin)行(xing)(xing)稀(xi)(xi)釋(shi)檢(jian)(jian)測抗體(ti)(ti)-HRP6mL3mL無(wu)(wu)20×洗(xi)滌(di)(di)(di)緩沖(chong)液(ye)(ye)(ye)(ye)20mL20mL按說(shuo)(shuo)明書進(jin)(jin)行(xing)(xing)稀(xi)(xi)釋(shi)底(di)物 A6mL3mL無(wu)(wu)底(di)物 B6mL3mL無(wu)(wu)終(zhong)止液(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)封(feng)板膜2 張(zhang)2 張(zhang)無(wu)(wu)說(shuo)(shuo)明書1 份(fen)1 份(fen)無(wu)(wu)自(zi)(zi)封(feng)袋1 個(ge)(ge)(ge)1 個(ge)(ge)(ge)無(wu)(wu) 操(cao)(cao)作步驟(zou)1. 準(zhun)備(bei)工作：將(jiang)試(shi)劑(ji)(ji)(ji)盒從冰箱(xiang)中(zhong)取出，室溫(wen)(wen)(wen)(wen)放置(zhi)(zhi)30分(fen)(fen)鐘，使試(shi)劑(ji)(ji)(ji)盒恢復(fu)(fu)至室溫(wen)(wen)(wen)(wen)。同時準(zhun)備(bei)好實(shi)(shi)驗所(suo)需(xu)的(de)(de)(de)(de)材料和(he)(he)器具。2. 加(jia)(jia)樣(yang)(yang)(yang)(yang)：在(zai)(zai)(zai)(zai)(zai)酶(mei)標(biao)(biao)(biao)(biao)(biao)板的(de)(de)(de)(de)弟1個(ge)(ge)(ge)孔(kong)(kong)(kong)中(zhong)加(jia)(jia)入(ru)(ru)標(biao)(biao)(biao)(biao)(biao)準(zhun)品50μL，然(ran)后(hou)在(zai)(zai)(zai)(zai)(zai)后(hou)續的(de)(de)(de)(de)孔(kong)(kong)(kong)中(zhong)依次加(jia)(jia)入(ru)(ru)待測樣(yang)(yang)(yang)(yang)本(ben)50μL。3. 溫(wen)(wen)(wen)(wen)育(yu)：將(jiang)酶(mei)標(biao)(biao)(biao)(biao)(biao)板密封(feng)，在(zai)(zai)(zai)(zai)(zai)37℃下(xia)溫(wen)(wen)(wen)(wen)育(yu)30分(fen)(fen)鐘。4. 洗(xi)滌(di)(di)(di)：用(yong)(yong)洗(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)清洗(xi)酶(mei)標(biao)(biao)(biao)(biao)(biao)板，共洗(xi)滌(di)(di)(di)5次。每(mei)(mei)次洗(xi)滌(di)(di)(di)后(hou)，用(yong)(yong)吸(xi)水紙(zhi)(zhi)輕(qing)輕(qing)吸(xi)干(gan)孔(kong)(kong)(kong)內液(ye)(ye)(ye)(ye)體(ti)(ti)。5. 加(jia)(jia)酶(mei)：在(zai)(zai)(zai)(zai)(zai)每(mei)(mei)個(ge)(ge)(ge)孔(kong)(kong)(kong)中(zhong)加(jia)(jia)入(ru)(ru)酶(mei)標(biao)(biao)(biao)(biao)(biao)物100μL。6. 溫(wen)(wen)(wen)(wen)育(yu)：再(zai)次將(jiang)酶(mei)標(biao)(biao)(biao)(biao)(biao)板密封(feng)，在(zai)(zai)(zai)(zai)(zai)37℃下(xia)溫(wen)(wen)(wen)(wen)育(yu)30分(fen)(fen)鐘。7. 洗(xi)滌(di)(di)(di)：用(yong)(yong)洗(xi)滌(di)(di)(di)液(ye)(ye)(ye)(ye)清洗(xi)酶(mei)標(biao)(biao)(biao)(biao)(biao)板，共洗(xi)滌(di)(di)(di)5次。每(mei)(mei)次洗(xi)滌(di)(di)(di)后(hou)，用(yong)(yong)吸(xi)水紙(zhi)(zhi)輕(qing)輕(qing)吸(xi)干(gan)孔(kong)(kong)(kong)內液(ye)(ye)(ye)(ye)體(ti)(ti)。8. 加(jia)(jia)底(di)物：在(zai)(zai)(zai)(zai)(zai)每(mei)(mei)個(ge)(ge)(ge)孔(kong)(kong)(kong)中(zhong)加(jia)(jia)入(ru)(ru)底(di)物A液(ye)(ye)(ye)(ye)50μL，再(zai)加(jia)(jia)入(ru)(ru)底(di)物B液(ye)(ye)(ye)(ye)50μL。9. 終(zhong)止反應(ying)：在(zai)(zai)(zai)(zai)(zai)每(mei)(mei)個(ge)(ge)(ge)孔(kong)(kong)(kong)中(zhong)加(jia)(jia)入(ru)(ru)終(zhong)止液(ye)(ye)(ye)(ye)50μL。10. 檢(jian)(jian)測：用(yong)(yong)酶(mei)標(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)(zai)(zai)(zai)450nm波長下(xia)讀取各(ge)孔(kong)(kong)(kong)的(de)(de)(de)(de)吸(xi)光(guang)度（OD值(zhi)）。 結果(guo)分(fen)(fen)析根據標(biao)(biao)(biao)(biao)(biao)準(zhun)品和(he)(he)樣(yang)(yang)(yang)(yang)本(ben)的(de)(de)(de)(de)OD值(zhi)，繪制標(biao)(biao)(biao)(biao)(biao)準(zhun)曲線，從而計算出樣(yang)(yang)(yang)(yang)本(ben)中(zhong)角蛋(dan)白1（KRT1）的(de)(de)(de)(de)濃度。具體(ti)(ti)分(fen)(fen)析方法可參考(kao)試(shi)劑(ji)(ji)(ji)盒說(shuo)(shuo)明書或相(xiang)關文獻。注意(yi)事項1. 在(zai)(zai)(zai)(zai)(zai)操(cao)(cao)作過程中(zhong)，應(ying)保(bao)(bao)持酶(mei)標(biao)(biao)(biao)(biao)(biao)板的(de)(de)(de)(de)干(gan)燥，避(bi)免(mian)(mian)水分(fen)(fen)進(jin)(jin)入(ru)(ru)孔(kong)(kong)(kong)內。2. 加(jia)(jia)樣(yang)(yang)(yang)(yang)時需(xu)確保(bao)(bao)加(jia)(jia)樣(yang)(yang)(yang)(yang)準(zhun)確、無(wu)(wu)氣(qi)泡產生。3. 溫(wen)(wen)(wen)(wen)育(yu)和(he)(he)洗(xi)滌(di)(di)(di)過程中(zhong)需(xu)嚴格控制時間和(he)(he)溫(wen)(wen)(wen)(wen)度，確保(bao)(bao)實(shi)(shi)驗結果(guo)的(de)(de)(de)(de)準(zhun)確性(xing)。4. 在(zai)(zai)(zai)(zai)(zai)使用(yong)(yong)酶(mei)標(biao)(biao)(biao)(biao)(biao)儀讀取OD值(zhi)時，需(xu)確保(bao)(bao)波長設(she)置(zhi)(zhi)正確，避(bi)免(mian)(mian)誤(wu)差產生。5. 在(zai)(zai)(zai)(zai)(zai)整個(ge)(ge)(ge)實(shi)(shi)驗過程中(zhong)，需(xu)保(bao)(bao)持操(cao)(cao)作環境的(de)(de)(de)(de)清潔(jie)和(he)(he)整潔(jie)，避(bi)免(mian)(mian)交(jiao)叉(cha)污(wu)染(ran)

                                 猴白介素8(IL-8)酶(mei)(mei)(mei)聯免(mian)疫試(shi)(shi)劑(ji)盒實(shi)(shi)(shi)驗(yan)原(yuan)(yuan)理(li)    ELISA運用(yong)(yong)了免(mian)疫學原(yuan)(yuan)理(li)和(he)化學反(fan)應(ying)顯(xian)色原(yuan)(yuan)理(li)，需(xu)(xu)要將(jiang)(jiang)抗(kang)原(yuan)(yuan)或(huo)抗(kang)體(ti)(ti)預包被(bei)在(zai)(zai)(zai)(zai)(zai)固相(xiang)載(zai)體(ti)(ti)表面，使后(hou)來(lai)形成的(de)(de)(de)(de)抗(kang)原(yuan)(yuan)-抗(kang)體(ti)(ti)-酶(mei)(mei)(mei) (熒光(guang)基團) -底(di)(di)(di)(di)物(wu)(wu)復(fu)合(he)物(wu)(wu)黏(nian)附在(zai)(zai)(zai)(zai)(zai)載(zai)體(ti)(ti)上，通過(guo)檢(jian)(jian)測(ce)OD值或(huo)發光(guang)值，來(lai)檢(jian)(jian)測(ce)靶蛋白的(de)(de)(de)(de)含(han)量。 實(shi)(shi)(shi)驗(yan)所需(xu)(xu)自備(bei)物(wu)(wu)品1. 酶(mei)(mei)(mei)標(biao)(biao)(biao)儀(yi)（450nm）2. 加(jia)(jia)樣器(qi)及槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒溫(wen)箱 試(shi)(shi)劑(ji)盒組成名稱96 孔(kong)(kong)(kong)(kong)配(pei)置(zhi)48 孔(kong)(kong)(kong)(kong)配(pei)置(zhi)備(bei)注(zhu)微(wei)孔(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)標(biao)(biao)(biao)板(ban)12 孔(kong)(kong)(kong)(kong)×8 條12 孔(kong)(kong)(kong)(kong)×4 條無標(biao)(biao)(biao)準(zhun)品0.5mL0.5mL按說(shuo)(shuo)明(ming)(ming)書進行稀(xi)釋(shi)標(biao)(biao)(biao)準(zhun)品稀(xi)釋(shi)液(ye)(ye)(ye)6mL3mL按說(shuo)(shuo)明(ming)(ming)書進行稀(xi)釋(shi)樣本稀(xi)釋(shi)液(ye)(ye)(ye)6mL3mL按說(shuo)(shuo)明(ming)(ming)書進行稀(xi)釋(shi)檢(jian)(jian)測(ce)抗(kang)體(ti)(ti)-HRP6mL3mL無20×洗(xi)滌(di)緩沖液(ye)(ye)(ye)20mL20mL按說(shuo)(shuo)明(ming)(ming)書進行稀(xi)釋(shi)底(di)(di)(di)(di)物(wu)(wu) A6mL3mL無底(di)(di)(di)(di)物(wu)(wu) B6mL3mL無終止(zhi)液(ye)(ye)(ye)6mL3mL無封(feng)板(ban)膜2 張2 張無說(shuo)(shuo)明(ming)(ming)書1 份1 份無自封(feng)袋1 個(ge)1 個(ge)無 操作步驟1. 準(zhun)備(bei)工作：將(jiang)(jiang)試(shi)(shi)劑(ji)盒從(cong)冰箱中(zhong)(zhong)(zhong)(zhong)取出，室溫(wen)放置(zhi)30分(fen)鐘，使試(shi)(shi)劑(ji)盒恢復(fu)至室溫(wen)。同時(shi)(shi)準(zhun)備(bei)好實(shi)(shi)(shi)驗(yan)所需(xu)(xu)的(de)(de)(de)(de)材料和(he)器(qi)具。2. 加(jia)(jia)樣：在(zai)(zai)(zai)(zai)(zai)酶(mei)(mei)(mei)標(biao)(biao)(biao)板(ban)的(de)(de)(de)(de)弟(di)1個(ge)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)入(ru)(ru)標(biao)(biao)(biao)準(zhun)品50μL，然后(hou)在(zai)(zai)(zai)(zai)(zai)后(hou)續(xu)的(de)(de)(de)(de)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)依次(ci)(ci)(ci)加(jia)(jia)入(ru)(ru)待(dai)測(ce)樣本50μL。3. 溫(wen)育：將(jiang)(jiang)酶(mei)(mei)(mei)標(biao)(biao)(biao)板(ban)密封(feng)，在(zai)(zai)(zai)(zai)(zai)37℃下(xia)溫(wen)育30分(fen)鐘。4. 洗(xi)滌(di)：用(yong)(yong)洗(xi)滌(di)液(ye)(ye)(ye)清洗(xi)酶(mei)(mei)(mei)標(biao)(biao)(biao)板(ban)，共(gong)洗(xi)滌(di)5次(ci)(ci)(ci)。每次(ci)(ci)(ci)洗(xi)滌(di)后(hou)，用(yong)(yong)吸(xi)水(shui)紙輕輕吸(xi)干孔(kong)(kong)(kong)(kong)內液(ye)(ye)(ye)體(ti)(ti)。5. 加(jia)(jia)酶(mei)(mei)(mei)：在(zai)(zai)(zai)(zai)(zai)每個(ge)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)入(ru)(ru)酶(mei)(mei)(mei)標(biao)(biao)(biao)物(wu)(wu)100μL。6. 溫(wen)育：再次(ci)(ci)(ci)將(jiang)(jiang)酶(mei)(mei)(mei)標(biao)(biao)(biao)板(ban)密封(feng)，在(zai)(zai)(zai)(zai)(zai)37℃下(xia)溫(wen)育30分(fen)鐘。7. 洗(xi)滌(di)：用(yong)(yong)洗(xi)滌(di)液(ye)(ye)(ye)清洗(xi)酶(mei)(mei)(mei)標(biao)(biao)(biao)板(ban)，共(gong)洗(xi)滌(di)5次(ci)(ci)(ci)。每次(ci)(ci)(ci)洗(xi)滌(di)后(hou)，用(yong)(yong)吸(xi)水(shui)紙輕輕吸(xi)干孔(kong)(kong)(kong)(kong)內液(ye)(ye)(ye)體(ti)(ti)。8. 加(jia)(jia)底(di)(di)(di)(di)物(wu)(wu)：在(zai)(zai)(zai)(zai)(zai)每個(ge)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)入(ru)(ru)底(di)(di)(di)(di)物(wu)(wu)A液(ye)(ye)(ye)50μL，再加(jia)(jia)入(ru)(ru)底(di)(di)(di)(di)物(wu)(wu)B液(ye)(ye)(ye)50μL。9. 終止(zhi)反(fan)應(ying)：在(zai)(zai)(zai)(zai)(zai)每個(ge)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)入(ru)(ru)終止(zhi)液(ye)(ye)(ye)50μL。10. 檢(jian)(jian)測(ce)：用(yong)(yong)酶(mei)(mei)(mei)標(biao)(biao)(biao)儀(yi)在(zai)(zai)(zai)(zai)(zai)450nm波長(chang)下(xia)讀取各孔(kong)(kong)(kong)(kong)的(de)(de)(de)(de)吸(xi)光(guang)度(du)（OD值）。 結(jie)(jie)果分(fen)析根(gen)據標(biao)(biao)(biao)準(zhun)品和(he)樣本的(de)(de)(de)(de)OD值，繪制標(biao)(biao)(biao)準(zhun)曲線(xian)，從(cong)而計算出樣本中(zhong)(zhong)(zhong)(zhong)角蛋白1（KRT1）的(de)(de)(de)(de)濃度(du)。具體(ti)(ti)分(fen)析方(fang)法可參考試(shi)(shi)劑(ji)盒說(shuo)(shuo)明(ming)(ming)書或(huo)相(xiang)關文(wen)獻。注(zhu)意事項1. 在(zai)(zai)(zai)(zai)(zai)操作過(guo)程中(zhong)(zhong)(zhong)(zhong)，應(ying)保(bao)持酶(mei)(mei)(mei)標(biao)(biao)(biao)板(ban)的(de)(de)(de)(de)干燥，避免(mian)水(shui)分(fen)進入(ru)(ru)孔(kong)(kong)(kong)(kong)內。2. 加(jia)(jia)樣時(shi)(shi)需(xu)(xu)確(que)(que)(que)保(bao)加(jia)(jia)樣準(zhun)確(que)(que)(que)、無氣泡產生。3. 溫(wen)育和(he)洗(xi)滌(di)過(guo)程中(zhong)(zhong)(zhong)(zhong)需(xu)(xu)嚴格控制時(shi)(shi)間和(he)溫(wen)度(du)，確(que)(que)(que)保(bao)實(shi)(shi)(shi)驗(yan)結(jie)(jie)果的(de)(de)(de)(de)準(zhun)確(que)(que)(que)性。4. 在(zai)(zai)(zai)(zai)(zai)使用(yong)(yong)酶(mei)(mei)(mei)標(biao)(biao)(biao)儀(yi)讀取OD值時(shi)(shi)，需(xu)(xu)確(que)(que)(que)保(bao)波長(chang)設置(zhi)正確(que)(que)(que)，避免(mian)誤差產生。5. 在(zai)(zai)(zai)(zai)(zai)整個(ge)實(shi)(shi)(shi)驗(yan)過(guo)程中(zhong)(zhong)(zhong)(zhong)，需(xu)(xu)保(bao)持操作環境的(de)(de)(de)(de)清潔(jie)和(he)整潔(jie)，避免(mian)交叉污染。

                                 猴白(bai)(bai)介素6(IL-6)酶(mei)(mei)(mei)聯免(mian)疫(yi)試劑(ji)盒 實(shi)驗原理    ELISA運用了(le)免(mian)疫(yi)學(xue)原理和(he)化學(xue)反應顯色(se)原理，需(xu)要將(jiang)(jiang)抗(kang)(kang)原或抗(kang)(kang)體(ti)(ti)(ti)預包被在(zai)(zai)固相(xiang)載(zai)體(ti)(ti)(ti)表面，使(shi)后(hou)來形成的(de)(de)抗(kang)(kang)原-抗(kang)(kang)體(ti)(ti)(ti)-酶(mei)(mei)(mei) (熒光基團) -底物(wu)復合物(wu)黏附在(zai)(zai)載(zai)體(ti)(ti)(ti)上(shang)，通過檢測(ce)OD值(zhi)(zhi)(zhi)或發光值(zhi)(zhi)(zhi)，來檢測(ce)靶蛋白(bai)(bai)的(de)(de)含量。 實(shi)驗所需(xu)自備物(wu)品(pin)(pin)1. 酶(mei)(mei)(mei)標儀（450nm）2. 加(jia)(jia)(jia)(jia)(jia)樣(yang)器及槍頭(tou)：0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒溫(wen)箱(xiang)(xiang) 試劑(ji)盒組(zu)成名稱96 孔(kong)(kong)(kong)配(pei)置(zhi)(zhi)48 孔(kong)(kong)(kong)配(pei)置(zhi)(zhi)備注微孔(kong)(kong)(kong)酶(mei)(mei)(mei)標板(ban)12 孔(kong)(kong)(kong)×8 條12 孔(kong)(kong)(kong)×4 條無(wu)(wu)標準(zhun)(zhun)品(pin)(pin)0.5mL0.5mL按(an)說明(ming)書(shu)進(jin)(jin)行(xing)(xing)(xing)稀釋(shi)標準(zhun)(zhun)品(pin)(pin)稀釋(shi)液(ye)(ye)(ye)6mL3mL按(an)說明(ming)書(shu)進(jin)(jin)行(xing)(xing)(xing)稀釋(shi)樣(yang)本稀釋(shi)液(ye)(ye)(ye)6mL3mL按(an)說明(ming)書(shu)進(jin)(jin)行(xing)(xing)(xing)稀釋(shi)檢測(ce)抗(kang)(kang)體(ti)(ti)(ti)-HRP6mL3mL無(wu)(wu)20×洗(xi)滌(di)(di)緩(huan)沖(chong)液(ye)(ye)(ye)20mL20mL按(an)說明(ming)書(shu)進(jin)(jin)行(xing)(xing)(xing)稀釋(shi)底物(wu) A6mL3mL無(wu)(wu)底物(wu) B6mL3mL無(wu)(wu)終止液(ye)(ye)(ye)6mL3mL無(wu)(wu)封板(ban)膜2 張(zhang)2 張(zhang)無(wu)(wu)說明(ming)書(shu)1 份(fen)(fen)1 份(fen)(fen)無(wu)(wu)自封袋1 個(ge)(ge)(ge)1 個(ge)(ge)(ge)無(wu)(wu) 操(cao)作(zuo)步驟1. 準(zhun)(zhun)備工(gong)作(zuo)：將(jiang)(jiang)試劑(ji)盒從冰箱(xiang)(xiang)中(zhong)(zhong)(zhong)取(qu)(qu)出，室溫(wen)放(fang)置(zhi)(zhi)30分鐘(zhong)，使(shi)試劑(ji)盒恢復至室溫(wen)。同時準(zhun)(zhun)備好實(shi)驗所需(xu)的(de)(de)材料和(he)器具。2. 加(jia)(jia)(jia)(jia)(jia)樣(yang)：在(zai)(zai)酶(mei)(mei)(mei)標板(ban)的(de)(de)弟1個(ge)(ge)(ge)孔(kong)(kong)(kong)中(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)入標準(zhun)(zhun)品(pin)(pin)50μL，然后(hou)在(zai)(zai)后(hou)續(xu)的(de)(de)孔(kong)(kong)(kong)中(zhong)(zhong)(zhong)依次(ci)加(jia)(jia)(jia)(jia)(jia)入待測(ce)樣(yang)本50μL。3. 溫(wen)育(yu)：將(jiang)(jiang)酶(mei)(mei)(mei)標板(ban)密(mi)封，在(zai)(zai)37℃下溫(wen)育(yu)30分鐘(zhong)。4. 洗(xi)滌(di)(di)：用洗(xi)滌(di)(di)液(ye)(ye)(ye)清(qing)(qing)洗(xi)酶(mei)(mei)(mei)標板(ban)，共洗(xi)滌(di)(di)5次(ci)。每(mei)次(ci)洗(xi)滌(di)(di)后(hou)，用吸(xi)(xi)水(shui)紙輕(qing)輕(qing)吸(xi)(xi)干(gan)孔(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)體(ti)(ti)(ti)。5. 加(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)(mei)：在(zai)(zai)每(mei)個(ge)(ge)(ge)孔(kong)(kong)(kong)中(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)入酶(mei)(mei)(mei)標物(wu)100μL。6. 溫(wen)育(yu)：再次(ci)將(jiang)(jiang)酶(mei)(mei)(mei)標板(ban)密(mi)封，在(zai)(zai)37℃下溫(wen)育(yu)30分鐘(zhong)。7. 洗(xi)滌(di)(di)：用洗(xi)滌(di)(di)液(ye)(ye)(ye)清(qing)(qing)洗(xi)酶(mei)(mei)(mei)標板(ban)，共洗(xi)滌(di)(di)5次(ci)。每(mei)次(ci)洗(xi)滌(di)(di)后(hou)，用吸(xi)(xi)水(shui)紙輕(qing)輕(qing)吸(xi)(xi)干(gan)孔(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)體(ti)(ti)(ti)。8. 加(jia)(jia)(jia)(jia)(jia)底物(wu)：在(zai)(zai)每(mei)個(ge)(ge)(ge)孔(kong)(kong)(kong)中(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)入底物(wu)A液(ye)(ye)(ye)50μL，再加(jia)(jia)(jia)(jia)(jia)入底物(wu)B液(ye)(ye)(ye)50μL。9. 終止反應：在(zai)(zai)每(mei)個(ge)(ge)(ge)孔(kong)(kong)(kong)中(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)入終止液(ye)(ye)(ye)50μL。10. 檢測(ce)：用酶(mei)(mei)(mei)標儀在(zai)(zai)450nm波長(chang)下讀(du)取(qu)(qu)各孔(kong)(kong)(kong)的(de)(de)吸(xi)(xi)光度（OD值(zhi)(zhi)(zhi)）。 結果分析(xi)根據標準(zhun)(zhun)品(pin)(pin)和(he)樣(yang)本的(de)(de)OD值(zhi)(zhi)(zhi)，繪(hui)制標準(zhun)(zhun)曲線，從而(er)計算出樣(yang)本中(zhong)(zhong)(zhong)角蛋白(bai)(bai)1（KRT1）的(de)(de)濃度。具體(ti)(ti)(ti)分析(xi)方法可參(can)考試劑(ji)盒說明(ming)書(shu)或相(xiang)關文(wen)獻。注意事(shi)項1. 在(zai)(zai)操(cao)作(zuo)過程(cheng)中(zhong)(zhong)(zhong)，應保(bao)(bao)持酶(mei)(mei)(mei)標板(ban)的(de)(de)干(gan)燥，避免(mian)水(shui)分進(jin)(jin)入孔(kong)(kong)(kong)內(nei)。2. 加(jia)(jia)(jia)(jia)(jia)樣(yang)時需(xu)確保(bao)(bao)加(jia)(jia)(jia)(jia)(jia)樣(yang)準(zhun)(zhun)確、無(wu)(wu)氣(qi)泡產生(sheng)。3. 溫(wen)育(yu)和(he)洗(xi)滌(di)(di)過程(cheng)中(zhong)(zhong)(zhong)需(xu)嚴格控(kong)制時間和(he)溫(wen)度，確保(bao)(bao)實(shi)驗結果的(de)(de)準(zhun)(zhun)確性。4. 在(zai)(zai)使(shi)用酶(mei)(mei)(mei)標儀讀(du)取(qu)(qu)OD值(zhi)(zhi)(zhi)時，需(xu)確保(bao)(bao)波長(chang)設(she)置(zhi)(zhi)正確，避免(mian)誤差產生(sheng)。5. 在(zai)(zai)整(zheng)個(ge)(ge)(ge)實(shi)驗過程(cheng)中(zhong)(zhong)(zhong)，需(xu)保(bao)(bao)持操(cao)作(zuo)環境的(de)(de)清(qing)(qing)潔(jie)和(he)整(zheng)潔(jie)，避免(mian)交叉(cha)污染(ran)。

實(shi)(shi)(shi)(shi)驗(yan)(yan)原理   試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙(shuang)抗(kang)(kang)(kang)體(ti)(ti)一步夾心法(fa)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)(yi)吸(xi)附試(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)（ELISA）。往預(yu)先(xian)包(bao)被白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)10(IL-10)捕獲抗(kang)(kang)(kang)體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包(bao)被微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，依(yi)(yi)次(ci)(ci)(ci)(ci)加(jia)(jia)(jia)入標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)測(ce)(ce)(ce)抗(kang)(kang)(kang)體(ti)(ti)，經過(guo)(guo)(guo)溫(wen)育并徹底洗(xi)滌。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)底物(wu)(wu)(wu)(wu)TMB顯(xian)(xian)色(se)(se)，TMB在(zai)(zai)(zai)(zai)(zai)(zai)(zai)過(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催(cui)化(hua)(hua)(hua)(hua)下(xia)(xia)轉(zhuan)化(hua)(hua)(hua)(hua)成(cheng)(cheng)藍(lan)(lan)色(se)(se)，并在(zai)(zai)(zai)(zai)(zai)(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)下(xia)(xia)轉(zhuan)化(hua)(hua)(hua)(hua)成(cheng)(cheng)＊終(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)(se)。顏色(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺(qian)和(he)(he)(he)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)10(IL-10)呈正相關。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)(zai)(zai)(zai)(zai)(zai)450nm 波長下(xia)(xia)測(ce)(ce)(ce)定(ding)(ding)吸(xi)光度(du)(du)(du)(du)(du)（OD 值(zhi)(zhi)(zhi)(zhi)(zhi)），計(ji)(ji)(ji)(ji)(ji)(ji)算樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)。保(bao)(bao)(bao)存(cun)(cun)(cun)條件(jian)及(ji)(ji)(ji)有(you)(you)(you)效(xiao)期(qi)1.本(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)體(ti)(ti)外定(ding)(ding)量(liang)檢(jian)(jian)測(ce)(ce)(ce)血(xue)(xue)清(qing)(qing)(qing)、血(xue)(xue)漿(jiang)、組(zu)織勻(yun)(yun)(yun)漿(jiang)及(ji)(ji)(ji)相關液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)10(IL-10)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)；2.試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)保(bao)(bao)(bao)存(cun)(cun)(cun)：2-8℃；3.有(you)(you)(you)效(xiao)期(qi)：6個月(yue)。猴(hou)白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)10(IL-10)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)(yi)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集及(ji)(ji)(ji)保(bao)(bao)(bao)存(cun)(cun)(cun)1.血(xue)(xue)清(qing)(qing)(qing)：全血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)請(qing)(qing)于(yu)(yu)(yu)(yu)室(shi)溫(wen)放(fang)置(zhi)(zhi)2小時(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)(huo)4℃過(guo)(guo)(guo)一晚(wan)后(hou)(hou)(hou)(hou)于(yu)(yu)(yu)(yu)1000 x g離(li)心20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，取上(shang)(shang)清(qing)(qing)(qing)即(ji)可檢(jian)(jian)測(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)存(cun)(cun)(cun)，但(dan)(dan)應(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)反(fan)復(fu)(fu)(fu)(fu)凍(dong)融(rong)。 2.血(xue)(xue)漿(jiang)：可用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)肝(gan)素(su)(su)(su)(su)(su)作(zuo)(zuo)(zuo)為(wei)(wei)抗(kang)(kang)(kang)凝劑(ji)(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)采(cai)集后(hou)(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)于(yu)(yu)(yu)(yu)2 - 8° C 1000 x g離(li)心15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)存(cun)(cun)(cun)，但(dan)(dan)應(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)反(fan)復(fu)(fu)(fu)(fu)凍(dong)融(rong)。 3.細胞培養物(wu)(wu)(wu)(wu)上(shang)(shang)清(qing)(qing)(qing)或(huo)(huo)(huo)(huo)其(qi)它生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)：1000 x g離(li)心20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，取上(shang)(shang)清(qing)(qing)(qing)即(ji)可檢(jian)(jian)測(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)存(cun)(cun)(cun)，但(dan)(dan)應(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)反(fan)復(fu)(fu)(fu)(fu)凍(dong)融(rong)。 (注(zhu)(zhu)(zhu)(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)溶(rong)(rong)(rong)(rong)血(xue)(xue)會影響**檢(jian)(jian)測(ce)(ce)(ce)結(jie)(jie)(jie)果(guo)(guo)(guo)(guo)，因此(ci)溶(rong)(rong)(rong)(rong)血(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)不宜(yi)進行(xing)此(ci)項檢(jian)(jian)測(ce)(ce)(ce)。) 猴(hou)白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)10(IL-10)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)(yi)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)   標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原則：首先(xian)通過(guo)(guo)(guo)文獻檢(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)式(shi)了解(jie)(jie)待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含量(liang)，確定(ding)(ding)適當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)。只有(you)(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍內(nei)(nei)，檢(jian)(jian)測(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)(jie)(jie)果(guo)(guo)(guo)(guo)才是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)，應(ying)(ying)(ying)(ying)做好(hao)詳(xiang)細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)(ji)(ji)錄。計(ji)(ji)(ji)(ji)(ji)(ji)算濃(nong)(nong)度(du)(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)應(ying)(ying)(ying)(ying)再(zai)乘以(yi)(yi)“N”。    標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原則：2瓶，每(mei)(mei)(mei)(mei)(mei)瓶臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前以(yi)(yi)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)至1ml，蓋好(hao)后(hou)(hou)(hou)(hou)靜(jing)置(zhi)(zhi)10分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)上(shang)(shang)，然(ran)后(hou)(hou)(hou)(hou)反(fan)復(fu)(fu)(fu)(fu)顛倒/搓動(dong)以(yi)(yi)助(zhu)溶(rong)(rong)(rong)(rong)解(jie)(jie)，其(qi)濃(nong)(nong)度(du)(du)(du)(du)(du)為(wei)(wei)300 pg/ml，做系(xi)列倍(bei)比稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)別稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)直接(jie)作(zuo)(zuo)(zuo)為(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)度(du)(du)(du)(du)(du)0 pg/ml，臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。 如(ru)(ru)(ru)(ru)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)：取0.5ml（不要(yao)(yao)(yao)(yao)少(shao)于(yu)(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)述(shu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)入含0.5ml樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)中(zhong)(zhong)，混(hun)勻(yun)(yun)(yun)即(ji)可，其(qi)余濃(nong)(nong)度(du)(du)(du)(du)(du)以(yi)(yi)此(ci)類推。    生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原則：臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前以(yi)(yi)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)前根(gen)(gen)據(ju)預(yu)先(xian)計(ji)(ji)(ji)(ji)(ji)(ji)算好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)(shi)(shi)驗(yan)(yan)所(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)(shi)際(ji)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)多配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)(ru)10μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)加(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕輕混(hun)勻(yun)(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)(zai)(zai)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前一小時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。    辣根(gen)(gen)過(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親和(he)(he)(he)素(su)(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原則：臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前以(yi)(yi)辣根(gen)(gen)過(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親和(he)(he)(he)素(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)前根(gen)(gen)據(ju)預(yu)先(xian)計(ji)(ji)(ji)(ji)(ji)(ji)算好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)(shi)(shi)驗(yan)(yan)所(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)(shi)際(ji)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)多配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)(ru)(ru)10μl辣根(gen)(gen)過(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親和(he)(he)(he)素(su)(su)(su)(su)(su)加(jia)(jia)(jia)990μl辣根(gen)(gen)過(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親和(he)(he)(he)素(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕輕混(hun)勻(yun)(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)(zai)(zai)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前一小時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。 猴(hou)白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)10(IL-10)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)(yi)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)操作(zuo)(zuo)(zuo)步驟   實(shi)(shi)(shi)(shi)驗(yan)(yan)開始前，請(qing)(qing)提前配(pei)(pei)置(zhi)(zhi)好(hao)所(suo)有(you)(you)(you)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)或(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)，均需(xu)混(hun)勻(yun)(yun)(yun)，混(hun)勻(yun)(yun)(yun)時(shi)(shi)(shi)(shi)(shi)盡(jin)量(liang)避(bi)(bi)免(mian)(mian)起泡。每(mei)(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)檢(jian)(jian)測(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)該(gai)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)。如(ru)(ru)(ru)(ru)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)過(guo)(guo)(guo)高(gao)時(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)進行(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)使樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)符合(he)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)測(ce)(ce)(ce)范圍。 1.加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)別設空白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。空白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別加(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)待測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)(zhu)(zhu)(zhu)意不要(yao)(yao)(yao)(yao)有(you)(you)(you)氣泡，加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)于(yu)(yu)(yu)(yu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部(bu)，盡(jin)量(liang)不觸及(ji)(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕輕晃動(dong)混(hun)勻(yun)(yun)(yun)，酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)(jia)上(shang)(shang)蓋或(huo)(huo)(huo)(huo)覆膜(mo)，37℃反(fan)應(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。 為(wei)(wei)保(bao)(bao)(bao)證(zheng)(zheng)實(shi)(shi)(shi)(shi)驗(yan)(yan)結(jie)(jie)(jie)果(guo)(guo)(guo)(guo)有(you)(you)(you)效(xiao)性，每(mei)(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)(shi)(shi)驗(yan)(yan)請(qing)(qing)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)。 2.棄去(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，甩干(gan)，不用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)滌。每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)工作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)加(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕輕混(hun)勻(yun)(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)(zai)(zai)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前一小時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。 3.溫(wen)育60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)，棄去(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，甩干(gan)，洗(xi)板(ban)(ban)(ban)3次(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)浸(jin)泡1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩干(gan)。4.每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)辣根(gen)(gen)過(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親和(he)(he)(he)素(su)(su)(su)(su)(su)工作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)（同(tong)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)工作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。5.溫(wen)育60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)，棄去(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，甩干(gan)，洗(xi)板(ban)(ban)(ban)5次(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)浸(jin)泡1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩干(gan)。6.依(yi)(yi)序(xu)每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)底物(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)(bi)光顯(xian)(xian)色(se)(se)（30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)，此(ci)時(shi)(shi)(shi)(shi)(shi)肉(rou)眼可見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)有(you)(you)(you)明顯(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)(du)(du)藍(lan)(lan)色(se)(se)，后(hou)(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)梯度(du)(du)(du)(du)(du)不明顯(xian)(xian)，即(ji)可終(zhong)(zhong)(zhong)止(zhi)(zhi)）。7.依(yi)(yi)序(xu)每(mei)(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)終(zhong)(zhong)(zhong)止(zhi)(zhi)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)(zhong)止(zhi)(zhi)反(fan)應(ying)(ying)(ying)(ying)（此(ci)時(shi)(shi)(shi)(shi)(shi)藍(lan)(lan)色(se)(se)立轉(zhuan)黃色(se)(se)）。終(zhong)(zhong)(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)入順(shun)序(xu)應(ying)(ying)(ying)(ying)盡(jin)量(liang)與(yu)底物(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)入順(shun)序(xu)相同(tong)。為(wei)(wei)了保(bao)(bao)(bao)證(zheng)(zheng)實(shi)(shi)(shi)(shi)驗(yan)(yan)結(jie)(jie)(jie)果(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性，底物(wu)(wu)(wu)(wu)反(fan)應(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)間到后(hou)(hou)(hou)(hou)應(ying)(ying)(ying)(ying)盡(jin)快加(jia)(jia)(jia)入終(zhong)(zhong)(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)儀在(zai)(zai)(zai)(zai)(zai)(zai)(zai)450nm波長依(yi)(yi)序(xu)測(ce)(ce)(ce)量(liang)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光密(mi)度(du)(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)(zhi)）。 在(zai)(zai)(zai)(zai)(zai)(zai)(zai)加(jia)(jia)(jia)終(zhong)(zhong)(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)內(nei)(nei)進行(xing)檢(jian)(jian)測(ce)(ce)(ce)。猴(hou)白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)10(IL-10)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)(yi)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)注(zhu)(zhu)(zhu)(zhu)意事(shi)(shi)項1.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)初次(ci)(ci)(ci)(ci)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)時(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)將(jiang)(jiang)(jiang)各(ge)種試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)管(guan)離(li)心數(shu)(shu)(shu)(shu)分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，以(yi)(yi)便試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)集中(zhong)(zhong)到管(guan)底。2.每(mei)(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)(shi)(shi)驗(yan)(yan)留一孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)(zuo)為(wei)(wei)空白(bai)(bai)調零孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，該(gai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不加(jia)(jia)(jia)任何試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，只是**加(jia)(jia)(jia)底物(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)及(ji)(ji)(ji)2N H2SO4。測(ce)(ce)(ce)量(liang)時(shi)(shi)(shi)(shi)(shi)先(xian)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)此(ci)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)調OD值(zhi)(zhi)(zhi)(zhi)(zhi)至零。 3.為(wei)(wei)防(fang)止(zhi)(zhi)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)時(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)(jiang)反(fan)應(ying)(ying)(ying)(ying)板(ban)(ban)(ban)放(fang)于(yu)(yu)(yu)(yu)鋪有(you)(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密(mi)閉盒(he)內(nei)(nei)，酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)(jia)上(shang)(shang)蓋或(huo)(huo)(huo)(huo)覆膜(mo)。 4.未(wei)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)或(huo)(huo)(huo)(huo)者(zhe)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)，請(qing)(qing)于(yu)(yu)(yu)(yu)2-8℃保(bao)(bao)(bao)存(cun)(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)工作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)、辣根(gen)(gen)過(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親和(he)(he)(he)素(su)(su)(su)(su)(su)工作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)請(qing)(qing)依(yi)(yi)據(ju)所(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)配(pei)(pei)置(zhi)(zhi)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)勿(wu)重復(fu)(fu)(fu)(fu)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)工作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)、辣根(gen)(gen)過(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親和(he)(he)(he)素(su)(su)(su)(su)(su)工作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)。 5.建議檢(jian)(jian)測(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)均設雙(shuang)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)定(ding)(ding)，以(yi)(yi)保(bao)(bao)(bao)證(zheng)(zheng)檢(jian)(jian)測(ce)(ce)(ce)結(jie)(jie)(jie)果(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性。 猴(hou)白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)10(IL-10)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)(yi)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)洗(xi)板(ban)(ban)(ban)方(fang)法(fa)手工洗(xi)板(ban)(ban)(ban)方(fang)法(fa)：吸(xi)去(qu)（不可觸及(ji)(ji)(ji)板(ban)(ban)(ban)壁）或(huo)(huo)(huo)(huo)甩掉(diao)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)內(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)；在(zai)(zai)(zai)(zai)(zai)(zai)(zai)實(shi)(shi)(shi)(shi)驗(yan)(yan)臺上(shang)(shang)鋪墊幾層(ceng)吸(xi)水紙(zhi)(zhi)，酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)朝(chao)下(xia)(xia)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)力拍幾次(ci)(ci)(ci)(ci)；將(jiang)(jiang)(jiang)推薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌緩沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)至少(shao)0.3ml注(zhu)(zhu)(zhu)(zhu)入孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)，浸(jin)泡1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。根(gen)(gen)據(ju)需(xu)要(yao)(yao)(yao)(yao)，重復(fu)(fu)(fu)(fu)此(ci)過(guo)(guo)(guo)程(cheng)(cheng)數(shu)(shu)(shu)(shu)次(ci)(ci)(ci)(ci)。 自(zi)動(dong)洗(xi)板(ban)(ban)(ban)：如(ru)(ru)(ru)(ru)果(guo)(guo)(guo)(guo)有(you)(you)(you)自(zi)動(dong)洗(xi)板(ban)(ban)(ban)機(ji)，應(ying)(ying)(ying)(ying)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)熟練使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)(hou)再(zai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)到正式(shi)實(shi)(shi)(shi)(shi)驗(yan)(yan)過(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)。 計(ji)(ji)(ji)(ji)(ji)(ji)算   以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)為(wei)(wei)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對(dui)數(shu)(shu)(shu)(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)(wei)縱(zong)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普(pu)通坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)(zai)(zai)(zai)(zai)半(ban)對(dui)數(shu)(shu)(shu)(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)(zhi)上(shang)(shang)繪(hui)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，根(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)查出(chu)(chu)(chu)相應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)；再(zai)乘以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)(zhi)(zhi)計(ji)(ji)(ji)(ji)(ji)(ji)算出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)回(hui)歸方(fang)程(cheng)(cheng)式(shi)，將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)代入方(fang)程(cheng)(cheng)式(shi)，計(ji)(ji)(ji)(ji)(ji)(ji)算出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)，再(zai)乘以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)，即(ji)為(wei)(wei)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)(shi)際(ji)濃(nong)(nong)度(du)(du)(du)(du)(du)。 注(zhu)(zhu)(zhu)(zhu)意事(shi)(shi)項1.當(dang)混(hun)合(he)蛋(dan)白(bai)(bai)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)盡(jin)量(liang)輕緩，避(bi)(bi)免(mian)(mian)起泡。2.洗(xi)滌過(guo)(guo)(guo)程(cheng)(cheng)非(fei)常重要(yao)(yao)(yao)(yao)，不充分(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌易(yi)造成(cheng)(cheng)假陽性。3.一次(ci)(ci)(ci)(ci)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)間**控(kong)制(zhi)(zhi)(zhi)(zhi)(zhi)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)，如(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)數(shu)(shu)(shu)(shu)量(liang)多，推薦使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)。4.請(qing)(qing)每(mei)(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)定(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi)(shi)(shi)(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，做復(fu)(fu)(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。5.如(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)待測(ce)(ce)(ce)物(wu)(wu)(wu)(wu)質含量(liang)過(guo)(guo)(guo)高(gao)，請(qing)(qing)先(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)再(zai)測(ce)(ce)(ce)定(ding)(ding)，計(ji)(ji)(ji)(ji)(ji)(ji)算時(shi)(shi)(shi)(shi)(shi)請(qing)(qing)乘以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)。6.在(zai)(zai)(zai)(zai)(zai)(zai)(zai)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)測(ce)(ce)(ce)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)工作(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)，請(qing)(qing)以(yi)(yi)相應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，不能混(hun)淆(xiao)。7.底物(wu)(wu)(wu)(wu)請(qing)(qing)避(bi)(bi)光保(bao)(bao)(bao)存(cun)(cun)(cun)。 8.不要(yao)(yao)(yao)(yao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)其(qi)它生(sheng)(sheng)(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)替換試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)。 注(zhu)(zhu)(zhu)(zhu)意事(shi)(shi)項：收集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)前必須清(qing)(qing)(qing)楚要(yao)(yao)(yao)(yao)檢(jian)(jian)測(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)份是否(fou)足夠穩定(ding)(ding)。對(dui)收集后(hou)(hou)(hou)(hou)當(dang)天進行(xing)檢(jian)(jian)測(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)，儲存(cun)(cun)(cun)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)(ru)(ru)有(you)(you)(you)特殊原因需(xu)要(yao)(yao)(yao)(yao)周期(qi)收集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)， 將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)及(ji)(ji)(ji)時(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)裝后(hou)(hou)(hou)(hou)放(fang)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)-70 ℃條件(jian)下(xia)(xia)保(bao)(bao)(bao)存(cun)(cun)(cun)。避(bi)(bi)免(mian)(mian)反(fan)復(fu)(fu)(fu)(fu)凍(dong)融(rong)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)2 -8 ℃可保(bao)(bao)(bao)存(cun)(cun)(cun)48 小時(shi)(shi)(shi)(shi)(shi)，-20 ℃可保(bao)(bao)(bao)存(cun)(cun)(cun)1 個月(yue)。-70度(du)(du)(du)(du)(du)可保(bao)(bao)(bao)存(cun)(cun)(cun)6 個月(yue)。部(bu)分(fen)(fen)(fen)(fen)激素(su)(su)(su)(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)(ben)需(xu)添加(jia)(jia)(jia)抑肽(tai)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)。計(ji)(ji)(ji)(ji)(ji)(ji)算：以(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)為(wei)(wei)橫坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)(wei)縱(zong)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)(zai)(zai)(zai)(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)(zhi)上(shang)(shang)繪(hui)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，根(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)查出(chu)(chu)(chu)相應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)；再(zai)乘以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)(zhi)(zhi)計(ji)(ji)(ji)(ji)(ji)(ji)算出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)回(hui)歸方(fang)程(cheng)(cheng)式(shi)，將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)代入方(fang)程(cheng)(cheng)式(shi)，計(ji)(ji)(ji)(ji)(ji)(ji)算出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)，再(zai)乘以(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)(shu)(shu)，即(ji)為(wei)(wei)樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)(shi)際(ji)濃(nong)(nong)度(du)(du)(du)(du)(du)。 猴(hou)白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)10(IL-10)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)(yi)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)性能1.樣(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)線(xian)(xian)(xian)性回(hui)歸與(yu)預(yu)期(qi)濃(nong)(nong)度(du)(du)(du)(du)(du)相關系(xi)數(shu)(shu)(shu)(shu)R值(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)(wei)0.92以(yi)(yi)上(shang)(shang)。2.批(pi)(pi)內(nei)(nei)與(yu)批(pi)(pi)見應(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)別小于(yu)(yu)(yu)(yu)9%和(he)(he)(he)15% 猴(hou)白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)10(IL-10)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)(yi)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)盒(he)

實(shi)(shi)驗(yan)原(yuan)理   試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)采用(yong)(yong)(yong)(yong)(yong)(yong)雙(shuang)(shuang)抗(kang)(kang)體(ti)(ti)(ti)一(yi)(yi)步夾心法(fa)酶(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)免(mian)(mian)(mian)疫(yi)(yi)吸附試(shi)(shi)(shi)驗(yan)（ELISA）。往預(yu)(yu)(yu)先(xian)(xian)包(bao)被白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)2(IL-2)捕獲抗(kang)(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包(bao)被微孔(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，依次加(jia)(jia)入(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)抗(kang)(kang)體(ti)(ti)(ti)，經(jing)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)溫育并徹底(di)(di)洗(xi)滌。用(yong)(yong)(yong)(yong)(yong)(yong)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯(xian)色(se)(se)(se)(se)，TMB在(zai)(zai)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)(hua)下(xia)(xia)(xia)(xia)轉(zhuan)化(hua)(hua)(hua)成藍(lan)色(se)(se)(se)(se)，并在(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)下(xia)(xia)(xia)(xia)轉(zhuan)化(hua)(hua)(hua)成＊終的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)色(se)(se)(se)(se)。顏色(se)(se)(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)2(IL-2)呈正相(xiang)(xiang)(xiang)關。用(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)450nm 波(bo)長(chang)下(xia)(xia)(xia)(xia)測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)吸光度(du)(du)(du)（OD 值(zhi)），計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)。保(bao)(bao)(bao)(bao)(bao)存(cun)條(tiao)件及(ji)有效(xiao)期1.本(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)體(ti)(ti)(ti)外(wai)定(ding)(ding)(ding)量(liang)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)血(xue)清(qing)、血(xue)漿、組織勻(yun)(yun)漿及(ji)相(xiang)(xiang)(xiang)關液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)本(ben)中(zhong)(zhong)白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)2(IL-2)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)；2.試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)保(bao)(bao)(bao)(bao)(bao)存(cun)：2-8℃；3.有效(xiao)期：6個月。猴白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)2(IL-2)酶(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)免(mian)(mian)(mian)疫(yi)(yi)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采集(ji)(ji)及(ji)保(bao)(bao)(bao)(bao)(bao)存(cun)1.血(xue)清(qing)：全血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)請(qing)于(yu)(yu)(yu)(yu)(yu)室溫放(fang)(fang)(fang)置2小時(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)(huo)(huo)4℃過(guo)(guo)(guo)(guo)(guo)(guo)(guo)一(yi)(yi)晚后(hou)于(yu)(yu)(yu)(yu)(yu)1000 x g離(li)心20分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，取上清(qing)即(ji)(ji)可(ke)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)放(fang)(fang)(fang)于(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)存(cun)，但(dan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)(bi)免(mian)(mian)(mian)反(fan)(fan)復凍融(rong)(rong)。 2.血(xue)漿：可(ke)用(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)(huo)肝素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)作(zuo)(zuo)為(wei)(wei)(wei)抗(kang)(kang)凝劑(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)采集(ji)(ji)后(hou)30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)于(yu)(yu)(yu)(yu)(yu)2 - 8° C 1000 x g離(li)心15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)放(fang)(fang)(fang)于(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)存(cun)，但(dan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)(bi)免(mian)(mian)(mian)反(fan)(fan)復凍融(rong)(rong)。 3.細胞培養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)上清(qing)或(huo)(huo)(huo)(huo)(huo)其(qi)它(ta)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)：1000 x g離(li)心20分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，取上清(qing)即(ji)(ji)可(ke)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)放(fang)(fang)(fang)于(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)存(cun)，但(dan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)(bi)免(mian)(mian)(mian)反(fan)(fan)復凍融(rong)(rong)。 (注(zhu)(zhu)(zhu)(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)溶(rong)(rong)血(xue)會影響**檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)結(jie)果(guo)(guo)，因此(ci)(ci)(ci)溶(rong)(rong)血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)不宜進(jin)行此(ci)(ci)(ci)項(xiang)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)。) 猴白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)2(IL-2)酶(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)免(mian)(mian)(mian)疫(yi)(yi)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)   標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則：首先(xian)(xian)通過(guo)(guo)(guo)(guo)(guo)(guo)(guo)文獻檢(jian)(jian)(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)式了(le)解待(dai)(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)本(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含量(liang)，確(que)定(ding)(ding)(ding)適當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)。只(zhi)有稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍內(nei)(nei)，檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果(guo)(guo)才是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)(cheng)中(zhong)(zhong)，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)做(zuo)(zuo)(zuo)好詳細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)錄。計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)濃(nong)(nong)(nong)(nong)度(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)了(le)“N”倍(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)再(zai)(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)“N”。    標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則：2瓶，每(mei)(mei)(mei)(mei)瓶臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)至1ml，蓋(gai)好后(hou)靜置10分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)上，然后(hou)反(fan)(fan)復顛倒/搓(cuo)動以(yi)(yi)(yi)(yi)(yi)(yi)(yi)助溶(rong)(rong)解，其(qi)濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)300 pg/ml，做(zuo)(zuo)(zuo)系列(lie)倍(bei)比(bi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)，分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)直(zhi)接作(zuo)(zuo)為(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)度(du)(du)(du)0 pg/ml，臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)配(pei)制(zhi)(zhi)(zhi)(zhi)。 如(ru)(ru)配(pei)制(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取0.5ml（不要(yao)(yao)(yao)少(shao)于(yu)(yu)(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上述(shu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)入(ru)(ru)含0.5ml樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中(zhong)(zhong)，混勻(yun)(yun)即(ji)(ji)可(ke)，其(qi)余濃(nong)(nong)(nong)(nong)度(du)(du)(du)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)此(ci)(ci)(ci)類推。    生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則：臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)根(gen)(gen)據(ju)預(yu)(yu)(yu)先(xian)(xian)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次實(shi)(shi)驗(yan)所需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)配(pei)制(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)際配(pei)制(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)多(duo)配(pei)制(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)加(jia)(jia)990μl生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)制(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)(qing)輕(qing)(qing)(qing)(qing)混勻(yun)(yun)，在(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)(yi)小時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)制(zhi)(zhi)(zhi)(zhi)。    辣根(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)和(he)(he)(he)(he)(he)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則：臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)辣根(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)和(he)(he)(he)(he)(he)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)根(gen)(gen)據(ju)預(yu)(yu)(yu)先(xian)(xian)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次實(shi)(shi)驗(yan)所需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)配(pei)制(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)際配(pei)制(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)多(duo)配(pei)制(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)(ru)10μl辣根(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)和(he)(he)(he)(he)(he)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)加(jia)(jia)990μl辣根(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)和(he)(he)(he)(he)(he)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)制(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)(qing)輕(qing)(qing)(qing)(qing)混勻(yun)(yun)，在(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)(yi)小時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)制(zhi)(zhi)(zhi)(zhi)。 猴白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)2(IL-2)酶(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)免(mian)(mian)(mian)疫(yi)(yi)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)操作(zuo)(zuo)步驟   實(shi)(shi)驗(yan)開始前(qian)(qian)，請(qing)提(ti)前(qian)(qian)配(pei)置好所有試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)或(huo)(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)，均(jun)需(xu)(xu)混勻(yun)(yun)，混勻(yun)(yun)時(shi)(shi)(shi)(shi)(shi)(shi)盡(jin)量(liang)避(bi)(bi)(bi)免(mian)(mian)(mian)起泡(pao)(pao)。每(mei)(mei)(mei)(mei)次檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)(ying)(ying)(ying)該做(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)。如(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)高(gao)時(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)進(jin)行稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)(yi)(yi)(yi)使(shi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)符(fu)合(he)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)范圍。 1.加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別設(she)空白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)、待(dai)(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)。空白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別加(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)(huo)待(dai)(dai)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)(zhu)(zhu)(zhu)意(yi)不要(yao)(yao)(yao)有氣泡(pao)(pao)，加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)于(yu)(yu)(yu)(yu)(yu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)底(di)(di)部(bu)，盡(jin)量(liang)不觸(chu)及(ji)孔(kong)(kong)(kong)(kong)(kong)壁，輕(qing)(qing)(qing)(qing)輕(qing)(qing)(qing)(qing)晃動混勻(yun)(yun)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)上蓋(gai)或(huo)(huo)(huo)(huo)(huo)覆膜，37℃反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)保(bao)(bao)(bao)(bao)(bao)證實(shi)(shi)驗(yan)結(jie)果(guo)(guo)有效(xiao)性(xing)(xing)，每(mei)(mei)(mei)(mei)次實(shi)(shi)驗(yan)請(qing)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 2.棄去液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干，不用(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)滌。每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)加(jia)(jia)99μl生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例配(pei)制(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)(qing)輕(qing)(qing)(qing)(qing)混勻(yun)(yun)，在(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)(yi)小時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)配(pei)制(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。 3.溫育60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)，棄去孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干，洗(xi)板(ban)(ban)(ban)3次，每(mei)(mei)(mei)(mei)次浸泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)干。4.每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)辣根(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)和(he)(he)(he)(he)(he)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。5.溫育60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)，棄去孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)干，洗(xi)板(ban)(ban)(ban)5次，每(mei)(mei)(mei)(mei)次浸泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)干。6.依序每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)(bi)(bi)光顯(xian)色(se)(se)(se)(se)（30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)，此(ci)(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)肉眼可(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)有明顯(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯(ti)度(du)(du)(du)藍(lan)色(se)(se)(se)(se)，后(hou)3-4孔(kong)(kong)(kong)(kong)(kong)梯(ti)度(du)(du)(du)不明顯(xian)，即(ji)(ji)可(ke)終止(zhi)）。7.依序每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)終止(zhi)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終止(zhi)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)（此(ci)(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)藍(lan)色(se)(se)(se)(se)立轉(zhuan)黃(huang)色(se)(se)(se)(se)）。終止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)入(ru)(ru)順序應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)量(liang)與(yu)(yu)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)入(ru)(ru)順序相(xiang)(xiang)(xiang)同。為(wei)(wei)(wei)了(le)保(bao)(bao)(bao)(bao)(bao)證實(shi)(shi)驗(yan)結(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)，底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)間(jian)到(dao)(dao)后(hou)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)快加(jia)(jia)入(ru)(ru)終止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)儀在(zai)(zai)450nm波(bo)長(chang)依序測(ce)(ce)(ce)(ce)量(liang)各孔(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光密度(du)(du)(du)（OD值(zhi)）。 在(zai)(zai)加(jia)(jia)終止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)內(nei)(nei)進(jin)行檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)。猴白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)2(IL-2)酶(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)免(mian)(mian)(mian)疫(yi)(yi)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)注(zhu)(zhu)(zhu)(zhu)意(yi)事項(xiang)1.用(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)初次使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)時(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)將(jiang)(jiang)(jiang)(jiang)各種試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)管離(li)心數(shu)(shu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)集(ji)(ji)中(zhong)(zhong)到(dao)(dao)管底(di)(di)。2.每(mei)(mei)(mei)(mei)次實(shi)(shi)驗(yan)留(liu)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)為(wei)(wei)(wei)空白(bai)(bai)調(diao)零孔(kong)(kong)(kong)(kong)(kong)，該孔(kong)(kong)(kong)(kong)(kong)不加(jia)(jia)任(ren)何試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，只(zhi)是**加(jia)(jia)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)及(ji)2N H2SO4。測(ce)(ce)(ce)(ce)量(liang)時(shi)(shi)(shi)(shi)(shi)(shi)先(xian)(xian)用(yong)(yong)(yong)(yong)(yong)(yong)此(ci)(ci)(ci)孔(kong)(kong)(kong)(kong)(kong)調(diao)OD值(zhi)至零。 3.為(wei)(wei)(wei)防止(zhi)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發(fa)，試(shi)(shi)(shi)驗(yan)時(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)(jiang)(jiang)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)板(ban)(ban)(ban)放(fang)(fang)(fang)于(yu)(yu)(yu)(yu)(yu)鋪(pu)有濕布(bu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)(he)(he)內(nei)(nei)，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)上蓋(gai)或(huo)(huo)(huo)(huo)(huo)覆膜。 4.未(wei)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)或(huo)(huo)(huo)(huo)(huo)者(zhe)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)，請(qing)于(yu)(yu)(yu)(yu)(yu)2-8℃保(bao)(bao)(bao)(bao)(bao)存(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣根(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)和(he)(he)(he)(he)(he)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)請(qing)依據(ju)所需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)配(pei)置使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)勿(wu)重復使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)已(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)(huo)、辣根(gen)(gen)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親(qin)和(he)(he)(he)(he)(he)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5.建議檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)均(jun)設(she)雙(shuang)(shuang)孔(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，以(yi)(yi)(yi)(yi)(yi)(yi)(yi)保(bao)(bao)(bao)(bao)(bao)證檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)結(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)。 猴白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)2(IL-2)酶(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)免(mian)(mian)(mian)疫(yi)(yi)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)洗(xi)板(ban)(ban)(ban)方(fang)(fang)法(fa)手工(gong)(gong)洗(xi)板(ban)(ban)(ban)方(fang)(fang)法(fa)：吸去（不可(ke)觸(chu)及(ji)板(ban)(ban)(ban)壁）或(huo)(huo)(huo)(huo)(huo)甩(shuai)掉酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)內(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)；在(zai)(zai)實(shi)(shi)驗(yan)臺上鋪(pu)墊幾層吸水紙，酶(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)朝下(xia)(xia)(xia)(xia)用(yong)(yong)(yong)(yong)(yong)(yong)力(li)拍幾次；將(jiang)(jiang)(jiang)(jiang)推薦(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌緩沖液(ye)(ye)(ye)(ye)(ye)(ye)(ye)至少(shao)0.3ml注(zhu)(zhu)(zhu)(zhu)入(ru)(ru)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)，浸泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。根(gen)(gen)據(ju)需(xu)(xu)要(yao)(yao)(yao)，重復此(ci)(ci)(ci)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)(cheng)數(shu)(shu)次。 自(zi)動洗(xi)板(ban)(ban)(ban)：如(ru)(ru)果(guo)(guo)有自(zi)動洗(xi)板(ban)(ban)(ban)機，應(ying)(ying)(ying)(ying)(ying)(ying)(ying)在(zai)(zai)熟(shu)練使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)后(hou)再(zai)(zai)用(yong)(yong)(yong)(yong)(yong)(yong)到(dao)(dao)正式實(shi)(shi)驗(yan)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)(cheng)中(zhong)(zhong)。 計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)   以(yi)(yi)(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)橫(heng)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對(dui)數(shu)(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)為(wei)(wei)(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)半對(dui)數(shu)(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上繪(hui)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，根(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)查出(chu)(chu)相(xiang)(xiang)(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)；再(zai)(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)；或(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)與(yu)(yu)OD值(zhi)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)(xian)(xian)回(hui)歸方(fang)(fang)程(cheng)(cheng)(cheng)(cheng)(cheng)式，將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代(dai)入(ru)(ru)方(fang)(fang)程(cheng)(cheng)(cheng)(cheng)(cheng)式，計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)出(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)，再(zai)(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)，即(ji)(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際濃(nong)(nong)(nong)(nong)度(du)(du)(du)。 注(zhu)(zhu)(zhu)(zhu)意(yi)事項(xiang)1.當(dang)混合(he)蛋白(bai)(bai)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)量(liang)輕(qing)(qing)(qing)(qing)緩，避(bi)(bi)(bi)免(mian)(mian)(mian)起泡(pao)(pao)。2.洗(xi)滌過(guo)(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)(cheng)非常重要(yao)(yao)(yao)，不充分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌易(yi)造(zao)成假陽性(xing)(xing)。3.一(yi)(yi)次加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)間(jian)**控(kong)制(zhi)(zhi)(zhi)(zhi)在(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)數(shu)(shu)量(liang)多(duo)，推薦(jian)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)排(pai)槍(qiang)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)。4.請(qing)每(mei)(mei)(mei)(mei)次測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)(shi)(shi)(shi)做(zuo)(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，做(zuo)(zuo)(zuo)復孔(kong)(kong)(kong)(kong)(kong)。5.如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)中(zhong)(zhong)待(dai)(dai)測(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)過(guo)(guo)(guo)(guo)(guo)(guo)(guo)高(gao)，請(qing)先(xian)(xian)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)再(zai)(zai)測(ce)(ce)(ce)(ce)定(ding)(ding)(ding)，計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)時(shi)(shi)(shi)(shi)(shi)(shi)請(qing)乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)。6.在(zai)(zai)配(pei)制(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)溶(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)相(xiang)(xiang)(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)制(zhi)(zhi)(zhi)(zhi)，不能混淆。7.底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)避(bi)(bi)(bi)光保(bao)(bao)(bao)(bao)(bao)存(cun)。 8.不要(yao)(yao)(yao)用(yong)(yong)(yong)(yong)(yong)(yong)其(qi)它(ta)生(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)替換試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)。 注(zhu)(zhu)(zhu)(zhu)意(yi)事項(xiang)：收(shou)集(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)前(qian)(qian)必(bi)須清(qing)楚要(yao)(yao)(yao)檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成份是否足夠穩(wen)定(ding)(ding)(ding)。對(dui)收(shou)集(ji)(ji)后(hou)當(dang)天進(jin)行檢(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)，儲存(cun)在(zai)(zai)4 ℃備(bei)用(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)有特殊原(yuan)因需(xu)(xu)要(yao)(yao)(yao)周期收(shou)集(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)， 將(jiang)(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)及(ji)時(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝后(hou)放(fang)(fang)(fang)在(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)(huo)-70 ℃條(tiao)件下(xia)(xia)(xia)(xia)保(bao)(bao)(bao)(bao)(bao)存(cun)。避(bi)(bi)(bi)免(mian)(mian)(mian)反(fan)(fan)復凍融(rong)(rong)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)2 -8 ℃可(ke)保(bao)(bao)(bao)(bao)(bao)存(cun)48 小時(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)保(bao)(bao)(bao)(bao)(bao)存(cun)1 個月。-70度(du)(du)(du)可(ke)保(bao)(bao)(bao)(bao)(bao)存(cun)6 個月。部(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)激素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)需(xu)(xu)添加(jia)(jia)抑肽酶(mei)(mei)(mei)(mei)(mei)。計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)：以(yi)(yi)(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)橫(heng)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)為(wei)(wei)(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上繪(hui)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，根(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)查出(chu)(chu)相(xiang)(xiang)(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)；再(zai)(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)；或(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)與(yu)(yu)OD值(zhi)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)線(xian)(xian)(xian)回(hui)歸方(fang)(fang)程(cheng)(cheng)(cheng)(cheng)(cheng)式，將(jiang)(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)代(dai)入(ru)(ru)方(fang)(fang)程(cheng)(cheng)(cheng)(cheng)(cheng)式，計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)出(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)，再(zai)(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)數(shu)(shu)，即(ji)(ji)為(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)際濃(nong)(nong)(nong)(nong)度(du)(du)(du)。 猴白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)2(IL-2)酶(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)免(mian)(mian)(mian)疫(yi)(yi)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)性(xing)(xing)能1.樣(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)(xian)性(xing)(xing)回(hui)歸與(yu)(yu)預(yu)(yu)(yu)期濃(nong)(nong)(nong)(nong)度(du)(du)(du)相(xiang)(xiang)(xiang)關系數(shu)(shu)R值(zhi)為(wei)(wei)(wei)0.92以(yi)(yi)(yi)(yi)(yi)(yi)(yi)上。2.批內(nei)(nei)與(yu)(yu)批見應(ying)(ying)(ying)(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別小于(yu)(yu)(yu)(yu)(yu)9%和(he)(he)(he)(he)(he)15% 猴白(bai)(bai)介(jie)素(su)(su)(su)(su)(su)(su)(su)(su)(su)(su)2(IL-2)酶(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)免(mian)(mian)(mian)疫(yi)(yi)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)(he)(he)

實驗(yan)原(yuan)理   試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)采(cai)用(yong)(yong)(yong)(yong)雙抗(kang)(kang)體(ti)(ti)(ti)(ti)一(yi)(yi)(yi)步(bu)夾心法(fa)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)(mian)疫(yi)吸附試(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)（ELISA）。往(wang)預(yu)(yu)先(xian)(xian)(xian)(xian)包被白(bai)(bai)介(jie)(jie)素(su)(su)(su)12(IL-12)捕獲抗(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，依次(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)體(ti)(ti)(ti)(ti)，經過(guo)(guo)溫(wen)育(yu)并徹底(di)洗(xi)滌(di)。用(yong)(yong)(yong)(yong)底(di)物(wu)(wu)(wu)(wu)TMB顯色(se)，TMB在(zai)(zai)(zai)過(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)(hua)下(xia)轉(zhuan)(zhuan)化(hua)(hua)(hua)成(cheng)藍色(se)，并在(zai)(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)下(xia)轉(zhuan)(zhuan)化(hua)(hua)(hua)成(cheng)＊終(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)色(se)。顏色(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)(he)(he)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)白(bai)(bai)介(jie)(jie)素(su)(su)(su)12(IL-12)呈(cheng)正相(xiang)(xiang)(xiang)(xiang)關(guan)。用(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)(zai)450nm 波(bo)長下(xia)測(ce)(ce)(ce)(ce)(ce)定(ding)吸光(guang)度(du)(du)(du)(du)(du)（OD 值(zhi)(zhi)），計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)。保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)條件(jian)及(ji)(ji)有(you)(you)(you)效(xiao)期1.本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)體(ti)(ti)(ti)(ti)外定(ding)量(liang)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)血(xue)清、血(xue)漿(jiang)、組織勻(yun)(yun)漿(jiang)及(ji)(ji)相(xiang)(xiang)(xiang)(xiang)關(guan)液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)中(zhong)(zhong)白(bai)(bai)介(jie)(jie)素(su)(su)(su)12(IL-12)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)(han)量(liang)；2.試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)：2-8℃；3.有(you)(you)(you)效(xiao)期：6個月。猴(hou)白(bai)(bai)介(jie)(jie)素(su)(su)(su)12(IL-12)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)(mian)疫(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集(ji)及(ji)(ji)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)1.血(xue)清：全血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)請(qing)(qing)(qing)(qing)于(yu)(yu)(yu)(yu)(yu)室溫(wen)放置(zhi)2小(xiao)(xiao)時(shi)(shi)(shi)或(huo)(huo)(huo)4℃過(guo)(guo)一(yi)(yi)(yi)晚后(hou)(hou)(hou)(hou)于(yu)(yu)(yu)(yu)(yu)1000 x g離(li)心20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，取(qu)上(shang)清即(ji)可(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放于(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)，但應(ying)(ying)(ying)避免(mian)(mian)(mian)(mian)(mian)反(fan)(fan)(fan)(fan)復(fu)(fu)(fu)凍(dong)融。 2.血(xue)漿(jiang)：可(ke)(ke)用(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)肝素(su)(su)(su)作(zuo)(zuo)(zuo)(zuo)為(wei)抗(kang)(kang)凝劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)采(cai)集(ji)后(hou)(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)于(yu)(yu)(yu)(yu)(yu)2 - 8° C 1000 x g離(li)心15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，或(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放于(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)，但應(ying)(ying)(ying)避免(mian)(mian)(mian)(mian)(mian)反(fan)(fan)(fan)(fan)復(fu)(fu)(fu)凍(dong)融。 3.細(xi)胞培養物(wu)(wu)(wu)(wu)上(shang)清或(huo)(huo)(huo)其(qi)(qi)它生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)：1000 x g離(li)心20分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，取(qu)上(shang)清即(ji)可(ke)(ke)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放于(yu)(yu)(yu)(yu)(yu)-20℃或(huo)(huo)(huo)-80℃保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)，但應(ying)(ying)(ying)避免(mian)(mian)(mian)(mian)(mian)反(fan)(fan)(fan)(fan)復(fu)(fu)(fu)凍(dong)融。 (注(zhu)(zhu)(zhu)(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)溶(rong)(rong)(rong)血(xue)會影響**檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)結果，因此(ci)溶(rong)(rong)(rong)血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)不(bu)(bu)(bu)宜進(jin)行(xing)此(ci)項(xiang)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)。) 猴(hou)白(bai)(bai)介(jie)(jie)素(su)(su)(su)12(IL-12)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)(mian)疫(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)   標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)原(yuan)則：首先(xian)(xian)(xian)(xian)通過(guo)(guo)文獻檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)索(suo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)式(shi)了(le)解待(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含(han)(han)量(liang)，確(que)定(ding)適當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)。只有(you)(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍(wei)內(nei)，檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結果才是(shi)(shi)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)(zhong)，應(ying)(ying)(ying)做(zuo)好詳細(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)(ji)(ji)錄。計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)時(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)了(le)“N”倍(bei)(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)應(ying)(ying)(ying)再乘以(yi)(yi)(yi)(yi)“N”。    標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)原(yuan)則：2瓶，每(mei)(mei)(mei)(mei)瓶臨用(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)至1ml，蓋好后(hou)(hou)(hou)(hou)靜置(zhi)10分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)上(shang)，然后(hou)(hou)(hou)(hou)反(fan)(fan)(fan)(fan)復(fu)(fu)(fu)顛倒(dao)/搓動(dong)以(yi)(yi)(yi)(yi)助溶(rong)(rong)(rong)解，其(qi)(qi)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)為(wei)300 pg/ml，做(zuo)系列倍(bei)(bei)(bei)比(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)別(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)直接作(zuo)(zuo)(zuo)(zuo)為(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)前(qian)(qian)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。 如(ru)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取(qu)0.5ml（不(bu)(bu)(bu)要(yao)少(shao)于(yu)(yu)(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)含(han)(han)0.5ml樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)(guan)中(zhong)(zhong)，混(hun)(hun)勻(yun)(yun)即(ji)可(ke)(ke)，其(qi)(qi)余濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)以(yi)(yi)(yi)(yi)此(ci)類(lei)推(tui)(tui)。    生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)原(yuan)則：臨用(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)前(qian)(qian)根(gen)(gen)(gen)(gen)據預(yu)(yu)先(xian)(xian)(xian)(xian)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實驗(yan)所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)100μl），實際(ji)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)10μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)(hun)勻(yun)(yun)，在(zai)(zai)(zai)使用(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)(yi)(yi)小(xiao)(xiao)時(shi)(shi)(shi)內(nei)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。    辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親和(he)(he)(he)(he)素(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)原(yuan)則：臨用(yong)(yong)(yong)(yong)前(qian)(qian)以(yi)(yi)(yi)(yi)辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親和(he)(he)(he)(he)素(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)前(qian)(qian)根(gen)(gen)(gen)(gen)據預(yu)(yu)先(xian)(xian)(xian)(xian)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實驗(yan)所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)量(liang)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)100μl），實際(ji)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)應(ying)(ying)(ying)多配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)10μl辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親和(he)(he)(he)(he)素(su)(su)(su)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親和(he)(he)(he)(he)素(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)(hun)勻(yun)(yun)，在(zai)(zai)(zai)使用(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)(yi)(yi)小(xiao)(xiao)時(shi)(shi)(shi)內(nei)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。 猴(hou)白(bai)(bai)介(jie)(jie)素(su)(su)(su)12(IL-12)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)(mian)疫(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)操作(zuo)(zuo)(zuo)(zuo)步(bu)驟   實驗(yan)開始前(qian)(qian)，請(qing)(qing)(qing)(qing)提(ti)前(qian)(qian)配(pei)(pei)置(zhi)好所有(you)(you)(you)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)或(huo)(huo)(huo)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)時(shi)(shi)(shi)，均需(xu)混(hun)(hun)勻(yun)(yun)，混(hun)(hun)勻(yun)(yun)時(shi)(shi)(shi)盡(jin)量(liang)避免(mian)(mian)(mian)(mian)(mian)起泡(pao)。每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)該做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線。如(ru)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)過(guo)(guo)高(gao)時(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)進(jin)行(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)使樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)范圍(wei)。 1.加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)別(bie)設空(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)(kong)、待(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)。空(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)別(bie)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)待(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)(zhu)(zhu)(zhu)意(yi)不(bu)(bu)(bu)要(yao)有(you)(you)(you)氣(qi)泡(pao)，加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)將(jiang)(jiang)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)(yu)(yu)(yu)(yu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)底(di)部，盡(jin)量(liang)不(bu)(bu)(bu)觸(chu)及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)壁(bi)(bi)，輕(qing)輕(qing)晃動(dong)混(hun)(hun)勻(yun)(yun)，酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)蓋或(huo)(huo)(huo)覆膜，37℃反(fan)(fan)(fan)(fan)應(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 為(wei)保(bao)(bao)(bao)(bao)(bao)(bao)證(zheng)實驗(yan)結果有(you)(you)(you)效(xiao)性(xing)(xing)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實驗(yan)請(qing)(qing)(qing)(qing)使用(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)(rong)液(ye)(ye)(ye)。 2.棄(qi)(qi)去液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)干，不(bu)(bu)(bu)用(yong)(yong)(yong)(yong)洗(xi)滌(di)。每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye) 100μl（取(qu)1μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)輕(qing)混(hun)(hun)勻(yun)(yun)，在(zai)(zai)(zai)使用(yong)(yong)(yong)(yong)前(qian)(qian)一(yi)(yi)(yi)小(xiao)(xiao)時(shi)(shi)(shi)內(nei)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 3.溫(wen)育(yu)60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)，棄(qi)(qi)去孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)干，洗(xi)板(ban)(ban)3次(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)干。4.每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親和(he)(he)(he)(he)素(su)(su)(su)工(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)（同生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。5.溫(wen)育(yu)60分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)，棄(qi)(qi)去孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)干，洗(xi)板(ban)(ban)5次(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)干。6.依序(xu)每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)物(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)液(ye)(ye)(ye)90μl，37℃避光(guang)顯色(se)（30分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)，此(ci)時(shi)(shi)(shi)肉眼可(ke)(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)有(you)(you)(you)明顯的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)(du)(du)藍色(se)，后(hou)(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)梯度(du)(du)(du)(du)(du)不(bu)(bu)(bu)明顯，即(ji)可(ke)(ke)終(zhong)(zhong)(zhong)止(zhi)(zhi)(zhi)）。7.依序(xu)每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)(zhong)止(zhi)(zhi)(zhi)溶(rong)(rong)(rong)液(ye)(ye)(ye)50μl，終(zhong)(zhong)(zhong)止(zhi)(zhi)(zhi)反(fan)(fan)(fan)(fan)應(ying)(ying)(ying)（此(ci)時(shi)(shi)(shi)藍色(se)立轉(zhuan)(zhuan)黃(huang)色(se)）。終(zhong)(zhong)(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)順序(xu)應(ying)(ying)(ying)盡(jin)量(liang)與(yu)(yu)底(di)物(wu)(wu)(wu)(wu)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)順序(xu)相(xiang)(xiang)(xiang)(xiang)同。為(wei)了(le)保(bao)(bao)(bao)(bao)(bao)(bao)證(zheng)實驗(yan)結果的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)，底(di)物(wu)(wu)(wu)(wu)反(fan)(fan)(fan)(fan)應(ying)(ying)(ying)時(shi)(shi)(shi)間(jian)到(dao)(dao)后(hou)(hou)(hou)(hou)應(ying)(ying)(ying)盡(jin)快加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)終(zhong)(zhong)(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)儀在(zai)(zai)(zai)450nm波(bo)長依序(xu)測(ce)(ce)(ce)(ce)(ce)量(liang)各孔(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)密度(du)(du)(du)(du)(du)（OD值(zhi)(zhi)）。 在(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)(zhong)(zhong)止(zhi)(zhi)(zhi)液(ye)(ye)(ye)后(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)內(nei)進(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)。猴(hou)白(bai)(bai)介(jie)(jie)素(su)(su)(su)12(IL-12)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)(mian)疫(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)注(zhu)(zhu)(zhu)(zhu)意(yi)事項(xiang)1.用(yong)(yong)(yong)(yong)戶(hu)在(zai)(zai)(zai)初次(ci)(ci)(ci)(ci)使用(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)時(shi)(shi)(shi)，應(ying)(ying)(ying)將(jiang)(jiang)各種(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)管(guan)(guan)離(li)心數(shu)分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)集(ji)中(zhong)(zhong)到(dao)(dao)管(guan)(guan)底(di)。2.每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實驗(yan)留一(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)(zuo)(zuo)為(wei)空(kong)白(bai)(bai)調(diao)零孔(kong)(kong)(kong)(kong)(kong)(kong)，該孔(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)任何試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)，只是(shi)(shi)**加(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)物(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)液(ye)(ye)(ye)及(ji)(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)量(liang)時(shi)(shi)(shi)先(xian)(xian)(xian)(xian)用(yong)(yong)(yong)(yong)此(ci)孔(kong)(kong)(kong)(kong)(kong)(kong)調(diao)OD值(zhi)(zhi)至零。 3.為(wei)防止(zhi)(zhi)(zhi)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發(fa)，試(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)時(shi)(shi)(shi)將(jiang)(jiang)反(fan)(fan)(fan)(fan)應(ying)(ying)(ying)板(ban)(ban)放于(yu)(yu)(yu)(yu)(yu)鋪有(you)(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)(he)內(nei)，酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)蓋或(huo)(huo)(huo)覆膜。 4.未使用(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)或(huo)(huo)(huo)者試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)，請(qing)(qing)(qing)(qing)于(yu)(yu)(yu)(yu)(yu)2-8℃保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)、辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親和(he)(he)(he)(he)素(su)(su)(su)工(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)請(qing)(qing)(qing)(qing)依據所需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)配(pei)(pei)置(zhi)使用(yong)(yong)(yong)(yong)。請(qing)(qing)(qing)(qing)勿重復(fu)(fu)(fu)使用(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)過(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)抗(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)或(huo)(huo)(huo)、辣(la)根(gen)(gen)(gen)(gen)過(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)(ji)(ji)親和(he)(he)(he)(he)素(su)(su)(su)工(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)。 5.建議檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)均設雙孔(kong)(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)定(ding)，以(yi)(yi)(yi)(yi)保(bao)(bao)(bao)(bao)(bao)(bao)證(zheng)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)結果的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)(xing)。 猴(hou)白(bai)(bai)介(jie)(jie)素(su)(su)(su)12(IL-12)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)(mian)疫(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)洗(xi)板(ban)(ban)方(fang)(fang)法(fa)手(shou)工(gong)洗(xi)板(ban)(ban)方(fang)(fang)法(fa)：吸去（不(bu)(bu)(bu)可(ke)(ke)觸(chu)及(ji)(ji)板(ban)(ban)壁(bi)(bi)）或(huo)(huo)(huo)甩(shuai)掉酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)內(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)；在(zai)(zai)(zai)實驗(yan)臺上(shang)鋪墊(dian)幾層吸水紙(zhi)，酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)朝下(xia)用(yong)(yong)(yong)(yong)力拍幾次(ci)(ci)(ci)(ci)；將(jiang)(jiang)推(tui)(tui)薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌(di)緩(huan)沖液(ye)(ye)(ye)至少(shao)0.3ml注(zhu)(zhu)(zhu)(zhu)入(ru)(ru)(ru)孔(kong)(kong)(kong)(kong)(kong)(kong)內(nei)，浸泡(pao)1-2分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。根(gen)(gen)(gen)(gen)據需(xu)要(yao)，重復(fu)(fu)(fu)此(ci)過(guo)(guo)程(cheng)(cheng)(cheng)(cheng)數(shu)次(ci)(ci)(ci)(ci)。 自動(dong)洗(xi)板(ban)(ban)：如(ru)果有(you)(you)(you)自動(dong)洗(xi)板(ban)(ban)機，應(ying)(ying)(ying)在(zai)(zai)(zai)熟(shu)練使用(yong)(yong)(yong)(yong)后(hou)(hou)(hou)(hou)再用(yong)(yong)(yong)(yong)到(dao)(dao)正式(shi)實驗(yan)過(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)(zhong)。 計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)   以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)為(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)為(wei)縱(zong)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)半(ban)對數(shu)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)繪出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線，根(gen)(gen)(gen)(gen)據樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線查出(chu)(chu)(chu)相(xiang)(xiang)(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)；再乘以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)與(yu)(yu)OD值(zhi)(zhi)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線回(hui)歸方(fang)(fang)程(cheng)(cheng)(cheng)(cheng)式(shi)，將(jiang)(jiang)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)代(dai)入(ru)(ru)(ru)方(fang)(fang)程(cheng)(cheng)(cheng)(cheng)式(shi)，計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)，再乘以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)，即(ji)為(wei)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實際(ji)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)。 注(zhu)(zhu)(zhu)(zhu)意(yi)事項(xiang)1.當(dang)混(hun)(hun)合蛋白(bai)(bai)溶(rong)(rong)(rong)液(ye)(ye)(ye)時(shi)(shi)(shi)應(ying)(ying)(ying)盡(jin)量(liang)輕(qing)緩(huan)，避免(mian)(mian)(mian)(mian)(mian)起泡(pao)。2.洗(xi)滌(di)過(guo)(guo)程(cheng)(cheng)(cheng)(cheng)非常(chang)重要(yao)，不(bu)(bu)(bu)充分(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)滌(di)易造成(cheng)假(jia)陽性(xing)(xing)。3.一(yi)(yi)(yi)次(ci)(ci)(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)時(shi)(shi)(shi)間(jian)**控制(zhi)(zhi)(zhi)(zhi)(zhi)在(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)量(liang)多，推(tui)(tui)薦使用(yong)(yong)(yong)(yong)排槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)。4.請(qing)(qing)(qing)(qing)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線，做(zuo)復(fu)(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)。5.如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中(zhong)(zhong)待(dai)測(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)質含(han)(han)量(liang)過(guo)(guo)高(gao)，請(qing)(qing)(qing)(qing)先(xian)(xian)(xian)(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)再測(ce)(ce)(ce)(ce)(ce)定(ding)，計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)時(shi)(shi)(shi)請(qing)(qing)(qing)(qing)乘以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)。6.在(zai)(zai)(zai)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)(rong)液(ye)(ye)(ye)工(gong)作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)時(shi)(shi)(shi)，請(qing)(qing)(qing)(qing)以(yi)(yi)(yi)(yi)相(xiang)(xiang)(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)液(ye)(ye)(ye)配(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，不(bu)(bu)(bu)能混(hun)(hun)淆。7.底(di)物(wu)(wu)(wu)(wu)請(qing)(qing)(qing)(qing)避光(guang)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)。 8.不(bu)(bu)(bu)要(yao)用(yong)(yong)(yong)(yong)其(qi)(qi)它生(sheng)(sheng)(sheng)產(chan)廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)替(ti)換試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)。 注(zhu)(zhu)(zhu)(zhu)意(yi)事項(xiang)：收集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)前(qian)(qian)必(bi)須清楚要(yao)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)份(fen)是(shi)(shi)否足夠穩定(ding)。對收集(ji)后(hou)(hou)(hou)(hou)當(dang)天進(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)，儲存(cun)在(zai)(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)，如(ru)有(you)(you)(you)特殊原(yuan)因需(xu)要(yao)周期收集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)， 將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)及(ji)(ji)時(shi)(shi)(shi)分(fen)(fen)(fen)(fen)裝后(hou)(hou)(hou)(hou)放在(zai)(zai)(zai)-20 ℃或(huo)(huo)(huo)-70 ℃條件(jian)下(xia)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)。避免(mian)(mian)(mian)(mian)(mian)反(fan)(fan)(fan)(fan)復(fu)(fu)(fu)凍(dong)融。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)48 小(xiao)(xiao)時(shi)(shi)(shi)，-20 ℃可(ke)(ke)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)1 個月。-70度(du)(du)(du)(du)(du)可(ke)(ke)保(bao)(bao)(bao)(bao)(bao)(bao)存(cun)6 個月。部分(fen)(fen)(fen)(fen)激素(su)(su)(su)類(lei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)需(xu)添(tian)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)抑肽(tai)酶(mei)(mei)(mei)(mei)(mei)(mei)。計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)：以(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)為(wei)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)為(wei)縱(zong)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上(shang)繪出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線，根(gen)(gen)(gen)(gen)據樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線查出(chu)(chu)(chu)相(xiang)(xiang)(xiang)(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)；再乘以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)；或(huo)(huo)(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)與(yu)(yu)OD值(zhi)(zhi)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線回(hui)歸方(fang)(fang)程(cheng)(cheng)(cheng)(cheng)式(shi)，將(jiang)(jiang)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)代(dai)入(ru)(ru)(ru)方(fang)(fang)程(cheng)(cheng)(cheng)(cheng)式(shi)，計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)，再乘以(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)倍(bei)(bei)(bei)數(shu)，即(ji)為(wei)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實際(ji)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)。 猴(hou)白(bai)(bai)介(jie)(jie)素(su)(su)(su)12(IL-12)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)(mian)疫(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)性(xing)(xing)能1.樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線性(xing)(xing)回(hui)歸與(yu)(yu)預(yu)(yu)期濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)(du)相(xiang)(xiang)(xiang)(xiang)關(guan)系數(shu)R值(zhi)(zhi)為(wei)0.92以(yi)(yi)(yi)(yi)上(shang)。2.批內(nei)與(yu)(yu)批見應(ying)(ying)(ying)分(fen)(fen)(fen)(fen)別(bie)小(xiao)(xiao)于(yu)(yu)(yu)(yu)(yu)9%和(he)(he)(he)(he)15% 猴(hou)白(bai)(bai)介(jie)(jie)素(su)(su)(su)12(IL-12)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)(mian)疫(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)

實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)原(yuan)理   試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)采(cai)用(yong)(yong)(yong)(yong)(yong)雙(shuang)抗(kang)(kang)(kang)體(ti)(ti)(ti)一(yi)步夾心(xin)法(fa)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)疫(yi)(yi)(yi)吸(xi)附試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)（ELISA）。往預(yu)先(xian)(xian)包(bao)被(bei)腫(zhong)瘤(liu)(liu)(liu)壞(huai)(huai)死因(yin)(yin)(yin)(yin)(yin)子(zi)(zi)(zi)α(TNF-α)捕獲抗(kang)(kang)(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包(bao)被(bei)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，依(yi)次(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)入(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)體(ti)(ti)(ti)，經過(guo)(guo)(guo)(guo)(guo)(guo)溫(wen)育并徹(che)底(di)(di)(di)洗(xi)(xi)滌(di)。用(yong)(yong)(yong)(yong)(yong)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯(xian)(xian)(xian)色(se)(se)，TMB在(zai)(zai)過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)(hua)下(xia)轉化(hua)(hua)(hua)成藍(lan)(lan)色(se)(se)，并在(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)下(xia)轉化(hua)(hua)(hua)成＊終的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)(se)。顏色(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)腫(zhong)瘤(liu)(liu)(liu)壞(huai)(huai)死因(yin)(yin)(yin)(yin)(yin)子(zi)(zi)(zi)α(TNF-α)呈正相關。用(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)在(zai)(zai)450nm 波(bo)長(chang)下(xia)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)吸(xi)光度(du)(du)(du)（OD 值(zhi)(zhi)(zhi)），計(ji)(ji)(ji)(ji)算(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)。保(bao)(bao)存條件(jian)及(ji)(ji)有(you)(you)(you)效期1.本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)體(ti)(ti)(ti)外定(ding)量(liang)(liang)(liang)(liang)(liang)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)血(xue)清、血(xue)漿(jiang)、組織勻(yun)漿(jiang)及(ji)(ji)相關液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)中(zhong)腫(zhong)瘤(liu)(liu)(liu)壞(huai)(huai)死因(yin)(yin)(yin)(yin)(yin)子(zi)(zi)(zi)α(TNF-α)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)(liang)(liang)(liang)；2.試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)保(bao)(bao)存：2-8℃；3.有(you)(you)(you)效期：6個(ge)月(yue)。猴腫(zhong)瘤(liu)(liu)(liu)壞(huai)(huai)死因(yin)(yin)(yin)(yin)(yin)子(zi)(zi)(zi)α(TNF-α)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)疫(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集(ji)(ji)(ji)及(ji)(ji)保(bao)(bao)存1.血(xue)清：全(quan)血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)請(qing)(qing)(qing)(qing)于(yu)(yu)(yu)室溫(wen)放(fang)置(zhi)(zhi)(zhi)2小(xiao)(xiao)時(shi)(shi)(shi)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)4℃過(guo)(guo)(guo)(guo)(guo)(guo)一(yi)晚后(hou)(hou)(hou)(hou)于(yu)(yu)(yu)1000 x g離心(xin)20分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，取(qu)上(shang)(shang)(shang)清即(ji)可(ke)(ke)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)存，但應(ying)(ying)(ying)(ying)(ying)避(bi)免(mian)(mian)(mian)(mian)反(fan)(fan)復(fu)(fu)凍(dong)融。 2.血(xue)漿(jiang)：可(ke)(ke)用(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)肝素(su)(su)作(zuo)(zuo)(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)抗(kang)(kang)(kang)凝劑(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)采(cai)集(ji)(ji)(ji)后(hou)(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)于(yu)(yu)(yu)2 - 8° C 1000 x g離心(xin)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)存，但應(ying)(ying)(ying)(ying)(ying)避(bi)免(mian)(mian)(mian)(mian)反(fan)(fan)復(fu)(fu)凍(dong)融。 3.細胞培養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)(shang)(shang)清或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)其它生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)：1000 x g離心(xin)20分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，取(qu)上(shang)(shang)(shang)清即(ji)可(ke)(ke)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)存，但應(ying)(ying)(ying)(ying)(ying)避(bi)免(mian)(mian)(mian)(mian)反(fan)(fan)復(fu)(fu)凍(dong)融。 (注(zhu)(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)溶(rong)血(xue)會影響**檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)(guo)，因(yin)(yin)(yin)(yin)(yin)此(ci)(ci)(ci)溶(rong)血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)不(bu)(bu)(bu)宜進(jin)行此(ci)(ci)(ci)項(xiang)(xiang)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)。) 猴腫(zhong)瘤(liu)(liu)(liu)壞(huai)(huai)死因(yin)(yin)(yin)(yin)(yin)子(zi)(zi)(zi)α(TNF-α)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)疫(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)   標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：首先(xian)(xian)通過(guo)(guo)(guo)(guo)(guo)(guo)文獻(xian)檢(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)(fang)式(shi)了解待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大致含量(liang)(liang)(liang)(liang)(liang)，確定(ding)適(shi)當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)。只(zhi)有(you)(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范圍(wei)內(nei)(nei)，檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果(guo)(guo)才是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)，應(ying)(ying)(ying)(ying)(ying)做(zuo)好(hao)詳細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)錄(lu)。計(ji)(ji)(ji)(ji)算(suan)濃(nong)(nong)(nong)度(du)(du)(du)時(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)應(ying)(ying)(ying)(ying)(ying)再(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)“N”。    標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：2瓶，每(mei)(mei)(mei)瓶臨用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)至1ml，蓋好(hao)后(hou)(hou)(hou)(hou)靜置(zhi)(zhi)(zhi)10分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)(yi)上(shang)(shang)(shang)，然(ran)后(hou)(hou)(hou)(hou)反(fan)(fan)復(fu)(fu)顛倒/搓動(dong)(dong)以(yi)(yi)(yi)(yi)(yi)(yi)助溶(rong)解，其濃(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)(wei)300 pg/ml，做(zuo)系列倍(bei)(bei)比稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)直接(jie)作(zuo)(zuo)(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)度(du)(du)(du)0 pg/ml，臨用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)。 如(ru)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)：取(qu)0.5ml（不(bu)(bu)(bu)要(yao)(yao)少于(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)(shang)(shang)述(shu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)入(ru)(ru)(ru)含0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中(zhong)，混(hun)(hun)(hun)(hun)(hun)勻(yun)即(ji)可(ke)(ke)，其余(yu)(yu)濃(nong)(nong)(nong)度(du)(du)(du)以(yi)(yi)(yi)(yi)(yi)(yi)此(ci)(ci)(ci)類(lei)推(tui)。    生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：臨用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根據(ju)預(yu)先(xian)(xian)計(ji)(ji)(ji)(ji)算(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)所(suo)需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)(shi)際配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)時(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)多(duo)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)0.1-0.2ml。如(ru)10μl生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)加(jia)(jia)(jia)990μl生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混(hun)(hun)(hun)(hun)(hun)勻(yun)，在(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)小(xiao)(xiao)時(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)。    辣(la)(la)根過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)原(yuan)則(ze)：臨用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)辣(la)(la)根過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根據(ju)預(yu)先(xian)(xian)計(ji)(ji)(ji)(ji)算(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)所(suo)需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)(shi)際配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)時(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)多(duo)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)0.1-0.2ml。如(ru)10μl辣(la)(la)根過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)加(jia)(jia)(jia)990μl辣(la)(la)根過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混(hun)(hun)(hun)(hun)(hun)勻(yun)，在(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)小(xiao)(xiao)時(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)。 猴腫(zhong)瘤(liu)(liu)(liu)壞(huai)(huai)死因(yin)(yin)(yin)(yin)(yin)子(zi)(zi)(zi)α(TNF-α)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)疫(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)操作(zuo)(zuo)(zuo)(zuo)(zuo)步驟   實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)開始前(qian)(qian)(qian)，請(qing)(qing)(qing)(qing)提前(qian)(qian)(qian)配(pei)(pei)(pei)(pei)(pei)置(zhi)(zhi)(zhi)好(hao)所(suo)有(you)(you)(you)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)，均需(xu)(xu)混(hun)(hun)(hun)(hun)(hun)勻(yun)，混(hun)(hun)(hun)(hun)(hun)勻(yun)時(shi)(shi)(shi)盡量(liang)(liang)(liang)(liang)(liang)避(bi)免(mian)(mian)(mian)(mian)起泡(pao)。每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)都應(ying)(ying)(ying)(ying)(ying)該(gai)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)。如(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)過(guo)(guo)(guo)(guo)(guo)(guo)高時(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)進(jin)行稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)(yi)(yi)使(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)符合試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)范圍(wei)。 1.加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別設空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)、待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。空白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余(yu)(yu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別加(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)(zhu)意(yi)(yi)不(bu)(bu)(bu)要(yao)(yao)有(you)(you)(you)氣泡(pao)，加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)于(yu)(yu)(yu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)底(di)(di)(di)部，盡量(liang)(liang)(liang)(liang)(liang)不(bu)(bu)(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)(qing)輕(qing)(qing)晃動(dong)(dong)混(hun)(hun)(hun)(hun)(hun)勻(yun)，酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)(jia)上(shang)(shang)(shang)蓋或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)覆膜，37℃反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)(wei)保(bao)(bao)證(zheng)實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)結(jie)果(guo)(guo)有(you)(you)(you)效性(xing)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)請(qing)(qing)(qing)(qing)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 2.棄去(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)(shuai)(shuai)干(gan)(gan)，不(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)滌(di)。每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取(qu)1μl生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)加(jia)(jia)(jia)99μl生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混(hun)(hun)(hun)(hun)(hun)勻(yun)，在(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)小(xiao)(xiao)時(shi)(shi)(shi)內(nei)(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。 3.溫(wen)育60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)，棄去(qu)(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)(shuai)(shuai)干(gan)(gan)，洗(xi)(xi)板(ban)(ban)(ban)3次(ci)(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)(shuai)干(gan)(gan)。4.每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)辣(la)(la)根過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同(tong)(tong)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。5.溫(wen)育60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)，棄去(qu)(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)，甩(shuai)(shuai)(shuai)干(gan)(gan)，洗(xi)(xi)板(ban)(ban)(ban)5次(ci)(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)浸泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)(shuai)干(gan)(gan)。6.依(yi)序(xu)每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)光顯(xian)(xian)(xian)色(se)(se)（30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)，此(ci)(ci)(ci)時(shi)(shi)(shi)肉(rou)眼可(ke)(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)有(you)(you)(you)明(ming)(ming)顯(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)藍(lan)(lan)色(se)(se)，后(hou)(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)梯度(du)(du)(du)不(bu)(bu)(bu)明(ming)(ming)顯(xian)(xian)(xian)，即(ji)可(ke)(ke)終止(zhi)(zhi)(zhi)）。7.依(yi)序(xu)每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)終止(zhi)(zhi)(zhi)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終止(zhi)(zhi)(zhi)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)（此(ci)(ci)(ci)時(shi)(shi)(shi)藍(lan)(lan)色(se)(se)立轉黃色(se)(se)）。終止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)入(ru)(ru)(ru)順序(xu)應(ying)(ying)(ying)(ying)(ying)盡量(liang)(liang)(liang)(liang)(liang)與底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)入(ru)(ru)(ru)順序(xu)相同(tong)(tong)。為(wei)(wei)(wei)(wei)了保(bao)(bao)證(zheng)實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)結(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)，底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)時(shi)(shi)(shi)間(jian)到后(hou)(hou)(hou)(hou)應(ying)(ying)(ying)(ying)(ying)盡快加(jia)(jia)(jia)入(ru)(ru)(ru)終止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)儀(yi)在(zai)(zai)450nm波(bo)長(chang)依(yi)序(xu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)(liang)(liang)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光密度(du)(du)(du)（OD值(zhi)(zhi)(zhi)）。 在(zai)(zai)加(jia)(jia)(jia)終止(zhi)(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)(yi)內(nei)(nei)進(jin)行檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)。猴腫(zhong)瘤(liu)(liu)(liu)壞(huai)(huai)死因(yin)(yin)(yin)(yin)(yin)子(zi)(zi)(zi)α(TNF-α)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)疫(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)注(zhu)(zhu)意(yi)(yi)事項(xiang)(xiang)1.用(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)初次(ci)(ci)(ci)(ci)(ci)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)時(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)(ying)將(jiang)各種試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)管離心(xin)數(shu)(shu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)集(ji)(ji)(ji)中(zhong)到管底(di)(di)(di)。2.每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)留一(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)空白(bai)調(diao)零孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，該(gai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)加(jia)(jia)(jia)任(ren)何試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)，只(zhi)是**加(jia)(jia)(jia)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)及(ji)(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)(liang)(liang)時(shi)(shi)(shi)先(xian)(xian)用(yong)(yong)(yong)(yong)(yong)此(ci)(ci)(ci)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)調(diao)OD值(zhi)(zhi)(zhi)至零。 3.為(wei)(wei)(wei)(wei)防(fang)止(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)蒸(zheng)發，試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)時(shi)(shi)(shi)將(jiang)反(fan)(fan)應(ying)(ying)(ying)(ying)(ying)板(ban)(ban)(ban)放(fang)于(yu)(yu)(yu)鋪有(you)(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)(he)內(nei)(nei)，酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)(jia)上(shang)(shang)(shang)蓋或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)覆膜。 4.未使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)者試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)，請(qing)(qing)(qing)(qing)于(yu)(yu)(yu)2-8℃保(bao)(bao)存。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)(la)根過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)請(qing)(qing)(qing)(qing)依(yi)據(ju)所(suo)需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)(pei)置(zhi)(zhi)(zhi)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。請(qing)(qing)(qing)(qing)勿重復(fu)(fu)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)、辣(la)(la)根過(guo)(guo)(guo)(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)(he)素(su)(su)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5.建議檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)均設雙(shuang)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)，以(yi)(yi)(yi)(yi)(yi)(yi)保(bao)(bao)證(zheng)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)。 猴腫(zhong)瘤(liu)(liu)(liu)壞(huai)(huai)死因(yin)(yin)(yin)(yin)(yin)子(zi)(zi)(zi)α(TNF-α)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)疫(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)洗(xi)(xi)板(ban)(ban)(ban)方(fang)(fang)(fang)法(fa)手(shou)工(gong)(gong)(gong)洗(xi)(xi)板(ban)(ban)(ban)方(fang)(fang)(fang)法(fa)：吸(xi)去(qu)(qu)（不(bu)(bu)(bu)可(ke)(ke)觸及(ji)(ji)板(ban)(ban)(ban)壁）或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)甩(shuai)(shuai)(shuai)掉酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)內(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)；在(zai)(zai)實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)臺(tai)上(shang)(shang)(shang)鋪墊幾層吸(xi)水紙，酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)朝下(xia)用(yong)(yong)(yong)(yong)(yong)力拍幾次(ci)(ci)(ci)(ci)(ci)；將(jiang)推(tui)薦(jian)(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)緩(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)至少0.3ml注(zhu)(zhu)入(ru)(ru)(ru)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)，浸泡(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。根據(ju)需(xu)(xu)要(yao)(yao)，重復(fu)(fu)此(ci)(ci)(ci)過(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)數(shu)(shu)次(ci)(ci)(ci)(ci)(ci)。 自動(dong)(dong)洗(xi)(xi)板(ban)(ban)(ban)：如(ru)果(guo)(guo)有(you)(you)(you)自動(dong)(dong)洗(xi)(xi)板(ban)(ban)(ban)機，應(ying)(ying)(ying)(ying)(ying)在(zai)(zai)熟練(lian)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)(hou)再(zai)用(yong)(yong)(yong)(yong)(yong)到正式(shi)實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)過(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)。 計(ji)(ji)(ji)(ji)算(suan)   以(yi)(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)半對數(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)繪出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，根據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)查出(chu)(chu)相應(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)；再(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)；或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)與OD值(zhi)(zhi)(zhi)計(ji)(ji)(ji)(ji)算(suan)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)回(hui)(hui)歸(gui)方(fang)(fang)(fang)程(cheng)(cheng)(cheng)(cheng)式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)(ru)(ru)方(fang)(fang)(fang)程(cheng)(cheng)(cheng)(cheng)式(shi)，計(ji)(ji)(ji)(ji)算(suan)出(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)，再(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)，即(ji)為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)(shi)際濃(nong)(nong)(nong)度(du)(du)(du)。 注(zhu)(zhu)意(yi)(yi)事項(xiang)(xiang)1.當(dang)混(hun)(hun)(hun)(hun)(hun)合蛋(dan)白(bai)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)盡量(liang)(liang)(liang)(liang)(liang)輕(qing)(qing)緩(huan)，避(bi)免(mian)(mian)(mian)(mian)起泡(pao)。2.洗(xi)(xi)滌(di)過(guo)(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)非(fei)常重要(yao)(yao)，不(bu)(bu)(bu)充(chong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)易造成假陽性(xing)。3.一(yi)次(ci)(ci)(ci)(ci)(ci)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)間(jian)**控制(zhi)(zhi)在(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)(shu)量(liang)(liang)(liang)(liang)(liang)多(duo)，推(tui)薦(jian)(jian)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)排槍(qiang)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4.請(qing)(qing)(qing)(qing)每(mei)(mei)(mei)次(ci)(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)(tong)時(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，做(zuo)復(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。5.如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中(zhong)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)(liang)(liang)(liang)(liang)過(guo)(guo)(guo)(guo)(guo)(guo)高，請(qing)(qing)(qing)(qing)先(xian)(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)再(zai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)，計(ji)(ji)(ji)(ji)算(suan)時(shi)(shi)(shi)請(qing)(qing)(qing)(qing)乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)。6.在(zai)(zai)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)工(gong)(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)，請(qing)(qing)(qing)(qing)以(yi)(yi)(yi)(yi)(yi)(yi)相應(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)，不(bu)(bu)(bu)能(neng)(neng)混(hun)(hun)(hun)(hun)(hun)淆。7.底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)(qing)(qing)(qing)避(bi)光保(bao)(bao)存。 8.不(bu)(bu)(bu)要(yao)(yao)用(yong)(yong)(yong)(yong)(yong)其它生(sheng)(sheng)(sheng)(sheng)(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)替換(huan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)。 注(zhu)(zhu)意(yi)(yi)事項(xiang)(xiang)：收集(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)前(qian)(qian)(qian)必須(xu)清楚要(yao)(yao)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成份是否足夠穩定(ding)。對收集(ji)(ji)(ji)后(hou)(hou)(hou)(hou)當(dang)天進(jin)行檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)，儲存在(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)，如(ru)有(you)(you)(you)特殊原(yuan)因(yin)(yin)(yin)(yin)(yin)需(xu)(xu)要(yao)(yao)周期收集(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)， 將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)及(ji)(ji)時(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)后(hou)(hou)(hou)(hou)放(fang)在(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)-70 ℃條件(jian)下(xia)保(bao)(bao)存。避(bi)免(mian)(mian)(mian)(mian)反(fan)(fan)復(fu)(fu)凍(dong)融。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)保(bao)(bao)存48 小(xiao)(xiao)時(shi)(shi)(shi)，-20 ℃可(ke)(ke)保(bao)(bao)存1 個(ge)月(yue)。-70度(du)(du)(du)可(ke)(ke)保(bao)(bao)存6 個(ge)月(yue)。部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)激素(su)(su)類(lei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)需(xu)(xu)添加(jia)(jia)(jia)抑肽酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)。計(ji)(ji)(ji)(ji)算(suan)：以(yi)(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)(shang)(shang)繪出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)，根據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)查出(chu)(chu)相應(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)；再(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)；或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)度(du)(du)(du)與OD值(zhi)(zhi)(zhi)計(ji)(ji)(ji)(ji)算(suan)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)(xian)回(hui)(hui)歸(gui)方(fang)(fang)(fang)程(cheng)(cheng)(cheng)(cheng)式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)(ru)(ru)方(fang)(fang)(fang)程(cheng)(cheng)(cheng)(cheng)式(shi)，計(ji)(ji)(ji)(ji)算(suan)出(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)，再(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)，即(ji)為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)(shi)際濃(nong)(nong)(nong)度(du)(du)(du)。 猴腫(zhong)瘤(liu)(liu)(liu)壞(huai)(huai)死因(yin)(yin)(yin)(yin)(yin)子(zi)(zi)(zi)α(TNF-α)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)疫(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)性(xing)能(neng)(neng)1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)線(xian)(xian)(xian)性(xing)回(hui)(hui)歸(gui)與預(yu)期濃(nong)(nong)(nong)度(du)(du)(du)相關系數(shu)(shu)R值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)0.92以(yi)(yi)(yi)(yi)(yi)(yi)上(shang)(shang)(shang)。2.批內(nei)(nei)與批見應(ying)(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別小(xiao)(xiao)于(yu)(yu)(yu)9%和(he)(he)(he)15% 猴腫(zhong)瘤(liu)(liu)(liu)壞(huai)(huai)死因(yin)(yin)(yin)(yin)(yin)子(zi)(zi)(zi)α(TNF-α)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)(mian)疫(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)

實(shi)(shi)(shi)驗(yan)(yan)原理   試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)(he)采用(yong)(yong)(yong)(yong)(yong)雙抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)一步(bu)夾心(xin)(xin)(xin)法酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)疫吸附試(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)（ELISA）。往預(yu)(yu)先(xian)包被可(ke)(ke)(ke)(ke)(ke)(ke)(ke)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)性(xing)(xing)腫(zhong)(zhong)瘤(liu)壞(huai)(huai)死(si)因(yin)(yin)(yin)子(zi)(zi)-1(sTNF-1)捕獲抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，依次(ci)(ci)(ci)(ci)加(jia)入(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)，經過(guo)(guo)(guo)(guo)(guo)溫(wen)育并徹底(di)洗(xi)(xi)(xi)(xi)滌。用(yong)(yong)(yong)(yong)(yong)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯(xian)色(se)(se)(se)(se)，TMB在(zai)(zai)(zai)(zai)(zai)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)下轉(zhuan)化(hua)(hua)成藍色(se)(se)(se)(se)，并在(zai)(zai)(zai)(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)下轉(zhuan)化(hua)(hua)成＊終(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃(huang)色(se)(se)(se)(se)。顏色(se)(se)(se)(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺(qian)和(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)性(xing)(xing)腫(zhong)(zhong)瘤(liu)壞(huai)(huai)死(si)因(yin)(yin)(yin)子(zi)(zi)-1(sTNF-1)呈正(zheng)相(xiang)(xiang)關(guan)(guan)。用(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)在(zai)(zai)(zai)(zai)(zai)450nm 波長下測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)吸光(guang)度(du)(du)（OD 值(zhi)(zhi)(zhi)），計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)。保存(cun)條(tiao)件及(ji)(ji)有(you)(you)(you)效(xiao)(xiao)期(qi)1.本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)體(ti)(ti)(ti)(ti)外定(ding)量(liang)(liang)(liang)(liang)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)血(xue)(xue)(xue)清(qing)、血(xue)(xue)(xue)漿、組織勻(yun)(yun)漿及(ji)(ji)相(xiang)(xiang)關(guan)(guan)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)中(zhong)(zhong)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)性(xing)(xing)腫(zhong)(zhong)瘤(liu)壞(huai)(huai)死(si)因(yin)(yin)(yin)子(zi)(zi)-1(sTNF-1)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)(liang)(liang)；2.試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)(he)保存(cun)：2-8℃；3.有(you)(you)(you)效(xiao)(xiao)期(qi)：6個(ge)月(yue)。猴(hou)(hou)(hou)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)性(xing)(xing)腫(zhong)(zhong)瘤(liu)壞(huai)(huai)死(si)因(yin)(yin)(yin)子(zi)(zi)-1(sTNF-1)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)疫試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采集及(ji)(ji)保存(cun)1.血(xue)(xue)(xue)清(qing)：全血(xue)(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)請(qing)于(yu)(yu)(yu)室溫(wen)放(fang)(fang)置2小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)(huo)4℃過(guo)(guo)(guo)(guo)(guo)一晚后(hou)(hou)(hou)(hou)于(yu)(yu)(yu)1000 x g離(li)心(xin)(xin)(xin)20分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，取(qu)(qu)上清(qing)即(ji)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)(fang)于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保存(cun)，但應(ying)(ying)(ying)(ying)避(bi)免(mian)(mian)(mian)反(fan)(fan)(fan)(fan)復凍(dong)融(rong)。 2.血(xue)(xue)(xue)漿：可(ke)(ke)(ke)(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)肝素(su)(su)(su)作(zuo)(zuo)為(wei)(wei)(wei)(wei)抗(kang)(kang)(kang)凝劑(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)采集后(hou)(hou)(hou)(hou)30分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)于(yu)(yu)(yu)2 - 8° C 1000 x g離(li)心(xin)(xin)(xin)15分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)(fang)于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保存(cun)，但應(ying)(ying)(ying)(ying)避(bi)免(mian)(mian)(mian)反(fan)(fan)(fan)(fan)復凍(dong)融(rong)。 3.細胞培養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)上清(qing)或(huo)(huo)(huo)(huo)其它(ta)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)：1000 x g離(li)心(xin)(xin)(xin)20分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，取(qu)(qu)上清(qing)即(ji)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)(fang)于(yu)(yu)(yu)-20℃或(huo)(huo)(huo)(huo)-80℃保存(cun)，但應(ying)(ying)(ying)(ying)避(bi)免(mian)(mian)(mian)反(fan)(fan)(fan)(fan)復凍(dong)融(rong)。 (注(zhu)(zhu)(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)血(xue)(xue)(xue)會影響**檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)結果(guo)(guo)，因(yin)(yin)(yin)此(ci)(ci)(ci)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)血(xue)(xue)(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)不(bu)(bu)(bu)宜進(jin)行(xing)(xing)此(ci)(ci)(ci)項(xiang)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)。) 猴(hou)(hou)(hou)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)性(xing)(xing)腫(zhong)(zhong)瘤(liu)壞(huai)(huai)死(si)因(yin)(yin)(yin)子(zi)(zi)-1(sTNF-1)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)疫試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)(he)   標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則：首先(xian)通(tong)(tong)過(guo)(guo)(guo)(guo)(guo)文獻檢(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)式(shi)了解(jie)待測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大(da)致(zhi)含量(liang)(liang)(liang)(liang)，確定(ding)適當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)。只有(you)(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)至標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范(fan)圍內(nei)(nei)(nei)(nei)，檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結果(guo)(guo)才是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)(guo)程中(zhong)(zhong)，應(ying)(ying)(ying)(ying)做(zuo)好(hao)詳細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)錄。計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)(suan)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)了“N”倍，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)應(ying)(ying)(ying)(ying)再(zai)(zai)乘(cheng)(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)“N”。    標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則：2瓶，每(mei)(mei)(mei)(mei)瓶臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)至1ml，蓋好(hao)后(hou)(hou)(hou)(hou)靜置10分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)上，然(ran)后(hou)(hou)(hou)(hou)反(fan)(fan)(fan)(fan)復顛(dian)倒/搓動以(yi)(yi)(yi)(yi)(yi)助溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)解(jie)，其濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)為(wei)(wei)(wei)(wei)300 pg/ml，做(zuo)系列(lie)倍比(bi)(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)，分(fen)(fen)(fen)別(bie)(bie)(bie)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)直接作(zuo)(zuo)為(wei)(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)0 pg/ml，臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)15分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)。 如(ru)(ru)(ru)(ru)配(pei)(pei)(pei)(pei)制(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取(qu)(qu)0.5ml（不(bu)(bu)(bu)要(yao)(yao)少于(yu)(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上述(shu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)入(ru)(ru)含0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管(guan)(guan)(guan)中(zhong)(zhong)，混(hun)勻(yun)(yun)即(ji)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)，其余濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)以(yi)(yi)(yi)(yi)(yi)此(ci)(ci)(ci)類推。    生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則：臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)(gen)(gen)(gen)據(ju)(ju)預(yu)(yu)先(xian)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)所需(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)際配(pei)(pei)(pei)(pei)制(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)多配(pei)(pei)(pei)(pei)制(zhi)0.1-0.2ml。如(ru)(ru)(ru)(ru)10μl生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)990μl生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)例配(pei)(pei)(pei)(pei)制(zhi)，輕輕混(hun)勻(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)。    辣根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)(he)素(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)原則：臨(lin)(lin)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)辣根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)(he)素(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)(gen)(gen)(gen)據(ju)(ju)預(yu)(yu)先(xian)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)所需(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)制(zhi)（每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)際配(pei)(pei)(pei)(pei)制(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)多配(pei)(pei)(pei)(pei)制(zhi)0.1-0.2ml。如(ru)(ru)(ru)(ru)10μl辣根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)(he)素(su)(su)(su)加(jia)990μl辣根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)(he)素(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)例配(pei)(pei)(pei)(pei)制(zhi)，輕輕混(hun)勻(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)。 猴(hou)(hou)(hou)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)性(xing)(xing)腫(zhong)(zhong)瘤(liu)壞(huai)(huai)死(si)因(yin)(yin)(yin)子(zi)(zi)-1(sTNF-1)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)疫試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)(he)操作(zuo)(zuo)步(bu)驟   實(shi)(shi)(shi)驗(yan)(yan)開始前(qian)(qian)(qian)，請(qing)提前(qian)(qian)(qian)配(pei)(pei)(pei)(pei)置好(hao)所有(you)(you)(you)試(shi)(shi)(shi)(shi)(shi)劑(ji)，試(shi)(shi)(shi)(shi)(shi)劑(ji)或(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)，均需(xu)(xu)(xu)混(hun)勻(yun)(yun)，混(hun)勻(yun)(yun)時(shi)(shi)(shi)(shi)(shi)(shi)盡(jin)量(liang)(liang)(liang)(liang)避(bi)免(mian)(mian)(mian)起泡(pao)。每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)都(dou)應(ying)(ying)(ying)(ying)該(gai)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)(xian)。如(ru)(ru)(ru)(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)過(guo)(guo)(guo)(guo)(guo)高(gao)時(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)進(jin)行(xing)(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)(yi)使(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合(he)試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)范(fan)圍。 1.加(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)別(bie)(bie)(bie)設空(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)、待測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。空(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)(bie)(bie)加(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)待測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)(zhu)(zhu)意(yi)不(bu)(bu)(bu)要(yao)(yao)有(you)(you)(you)氣泡(pao)，加(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)于(yu)(yu)(yu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)底(di)部(bu)，盡(jin)量(liang)(liang)(liang)(liang)不(bu)(bu)(bu)觸(chu)及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕輕晃動混(hun)勻(yun)(yun)，酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板加(jia)上蓋或(huo)(huo)(huo)(huo)覆(fu)膜，37℃反(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)(wei)保證(zheng)實(shi)(shi)(shi)驗(yan)(yan)結果(guo)(guo)有(you)(you)(you)效(xiao)(xiao)性(xing)(xing)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)請(qing)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 2.棄(qi)去(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干，不(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)(xi)(xi)滌。每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取(qu)(qu)1μl生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)加(jia)99μl生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)例配(pei)(pei)(pei)(pei)制(zhi)，輕輕混(hun)勻(yun)(yun)，在(zai)(zai)(zai)(zai)(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)制(zhi)），37℃,60分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。 3.溫(wen)育60分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)，棄(qi)去(qu)(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干，洗(xi)(xi)(xi)(xi)板3次(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)浸(jin)(jin)泡(pao)1-2分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)干。4.每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)辣根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)(he)素(su)(su)(su)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。5.溫(wen)育60分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)，棄(qi)去(qu)(qu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干，洗(xi)(xi)(xi)(xi)板5次(ci)(ci)(ci)(ci)，每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)浸(jin)(jin)泡(pao)1-2分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)干。6.依序(xu)(xu)每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避(bi)光(guang)顯(xian)色(se)(se)(se)(se)（30分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)，此(ci)(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)肉眼可(ke)(ke)(ke)(ke)(ke)(ke)(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)有(you)(you)(you)明(ming)(ming)顯(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)藍色(se)(se)(se)(se)，后(hou)(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)梯度(du)(du)不(bu)(bu)(bu)明(ming)(ming)顯(xian)，即(ji)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)終(zhong)(zhong)(zhong)(zhong)止(zhi)）。7.依序(xu)(xu)每(mei)(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)終(zhong)(zhong)(zhong)(zhong)止(zhi)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)(zhong)(zhong)(zhong)止(zhi)反(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)（此(ci)(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)藍色(se)(se)(se)(se)立轉(zhuan)黃(huang)色(se)(se)(se)(se)）。終(zhong)(zhong)(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)入(ru)(ru)順(shun)序(xu)(xu)應(ying)(ying)(ying)(ying)盡(jin)量(liang)(liang)(liang)(liang)與(yu)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)入(ru)(ru)順(shun)序(xu)(xu)相(xiang)(xiang)同。為(wei)(wei)(wei)(wei)了保證(zheng)實(shi)(shi)(shi)驗(yan)(yan)結果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)，底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)間到(dao)后(hou)(hou)(hou)(hou)應(ying)(ying)(ying)(ying)盡(jin)快加(jia)入(ru)(ru)終(zhong)(zhong)(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)儀(yi)在(zai)(zai)(zai)(zai)(zai)450nm波長依序(xu)(xu)測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)(liang)各孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)密度(du)(du)（OD值(zhi)(zhi)(zhi)）。 在(zai)(zai)(zai)(zai)(zai)加(jia)終(zhong)(zhong)(zhong)(zhong)止(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)內(nei)(nei)(nei)(nei)進(jin)行(xing)(xing)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)。猴(hou)(hou)(hou)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)性(xing)(xing)腫(zhong)(zhong)瘤(liu)壞(huai)(huai)死(si)因(yin)(yin)(yin)子(zi)(zi)-1(sTNF-1)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)疫試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)(he)注(zhu)(zhu)(zhu)意(yi)事項(xiang)1.用(yong)(yong)(yong)(yong)(yong)戶(hu)在(zai)(zai)(zai)(zai)(zai)初次(ci)(ci)(ci)(ci)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)(he)時(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)將(jiang)各種(zhong)試(shi)(shi)(shi)(shi)(shi)劑(ji)管(guan)(guan)(guan)離(li)心(xin)(xin)(xin)數(shu)(shu)(shu)分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)(shi)(shi)劑(ji)集中(zhong)(zhong)到(dao)管(guan)(guan)(guan)底(di)。2.每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)實(shi)(shi)(shi)驗(yan)(yan)留一孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)為(wei)(wei)(wei)(wei)空(kong)白(bai)(bai)調(diao)零孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，該(gai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)加(jia)任何(he)試(shi)(shi)(shi)(shi)(shi)劑(ji)，只是**加(jia)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)及(ji)(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)(liang)時(shi)(shi)(shi)(shi)(shi)(shi)先(xian)用(yong)(yong)(yong)(yong)(yong)此(ci)(ci)(ci)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)調(diao)OD值(zhi)(zhi)(zhi)至零。 3.為(wei)(wei)(wei)(wei)防止(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸發，試(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)時(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)反(fan)(fan)(fan)(fan)應(ying)(ying)(ying)(ying)板放(fang)(fang)于(yu)(yu)(yu)鋪有(you)(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密閉盒(he)(he)(he)(he)內(nei)(nei)(nei)(nei)，酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板加(jia)上蓋或(huo)(huo)(huo)(huo)覆(fu)膜。 4.未使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板或(huo)(huo)(huo)(huo)者試(shi)(shi)(shi)(shi)(shi)劑(ji)，請(qing)于(yu)(yu)(yu)2-8℃保存(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)(he)素(su)(su)(su)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)請(qing)依據(ju)(ju)所需(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)置使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。請(qing)勿重復使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)、辣根(gen)(gen)(gen)(gen)過(guo)(guo)(guo)(guo)(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)親和(he)(he)(he)素(su)(su)(su)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5.建(jian)議檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)均設雙孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)，以(yi)(yi)(yi)(yi)(yi)保證(zheng)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)結果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)。 猴(hou)(hou)(hou)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)性(xing)(xing)腫(zhong)(zhong)瘤(liu)壞(huai)(huai)死(si)因(yin)(yin)(yin)子(zi)(zi)-1(sTNF-1)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)疫試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)(he)洗(xi)(xi)(xi)(xi)板方(fang)(fang)法手工(gong)洗(xi)(xi)(xi)(xi)板方(fang)(fang)法：吸去(qu)(qu)（不(bu)(bu)(bu)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)觸(chu)及(ji)(ji)板壁）或(huo)(huo)(huo)(huo)甩(shuai)(shuai)掉酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板內(nei)(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)；在(zai)(zai)(zai)(zai)(zai)實(shi)(shi)(shi)驗(yan)(yan)臺上鋪墊幾層吸水紙(zhi)，酶(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板朝下用(yong)(yong)(yong)(yong)(yong)力拍幾次(ci)(ci)(ci)(ci)；將(jiang)推薦的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)(xi)(xi)滌緩(huan)沖液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)至少0.3ml注(zhu)(zhu)(zhu)入(ru)(ru)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)，浸(jin)(jin)泡(pao)1-2分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)。根(gen)(gen)(gen)(gen)據(ju)(ju)需(xu)(xu)(xu)要(yao)(yao)，重復此(ci)(ci)(ci)過(guo)(guo)(guo)(guo)(guo)程數(shu)(shu)(shu)次(ci)(ci)(ci)(ci)。 自(zi)動洗(xi)(xi)(xi)(xi)板：如(ru)(ru)(ru)(ru)果(guo)(guo)有(you)(you)(you)自(zi)動洗(xi)(xi)(xi)(xi)板機，應(ying)(ying)(ying)(ying)在(zai)(zai)(zai)(zai)(zai)熟練使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)(hou)再(zai)(zai)用(yong)(yong)(yong)(yong)(yong)到(dao)正(zheng)式(shi)實(shi)(shi)(shi)驗(yan)(yan)過(guo)(guo)(guo)(guo)(guo)程中(zhong)(zhong)。 計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)(suan)   以(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對(dui)數(shu)(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通(tong)(tong)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)(zai)(zai)半對(dui)數(shu)(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)(xian)，根(gen)(gen)(gen)(gen)據(ju)(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)(xian)查(cha)出(chu)相(xiang)(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)；再(zai)(zai)乘(cheng)(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)回(hui)歸方(fang)(fang)程式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)(ru)方(fang)(fang)程式(shi)，計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)，再(zai)(zai)乘(cheng)(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)，即(ji)為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)。 注(zhu)(zhu)(zhu)意(yi)事項(xiang)1.當(dang)混(hun)合(he)蛋白(bai)(bai)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)盡(jin)量(liang)(liang)(liang)(liang)輕緩(huan)，避(bi)免(mian)(mian)(mian)起泡(pao)。2.洗(xi)(xi)(xi)(xi)滌過(guo)(guo)(guo)(guo)(guo)程非常(chang)重要(yao)(yao)，不(bu)(bu)(bu)充分(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)(xi)(xi)滌易造成假陽(yang)性(xing)(xing)。3.一次(ci)(ci)(ci)(ci)加(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)間**控制(zhi)在(zai)(zai)(zai)(zai)(zai)5分(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)，如(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)多，推薦使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)排(pai)槍加(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4.請(qing)每(mei)(mei)(mei)(mei)次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)(xian)，做(zuo)復孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)。5.如(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中(zhong)(zhong)待測(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)(liang)(liang)(liang)過(guo)(guo)(guo)(guo)(guo)高(gao)，請(qing)先(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)再(zai)(zai)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)，計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)(suan)時(shi)(shi)(shi)(shi)(shi)(shi)請(qing)乘(cheng)(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)。6.在(zai)(zai)(zai)(zai)(zai)配(pei)(pei)(pei)(pei)制(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)工(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)以(yi)(yi)(yi)(yi)(yi)相(xiang)(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)制(zhi)，不(bu)(bu)(bu)能混(hun)淆。7.底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)避(bi)光(guang)保存(cun)。 8.不(bu)(bu)(bu)要(yao)(yao)用(yong)(yong)(yong)(yong)(yong)其它(ta)生(sheng)(sheng)(sheng)(sheng)產廠家的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)替換試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)(he)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)劑(ji)。 注(zhu)(zhu)(zhu)意(yi)事項(xiang)：收(shou)(shou)集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)前(qian)(qian)(qian)必須清(qing)楚要(yao)(yao)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成份是否足(zu)夠穩定(ding)。對(dui)收(shou)(shou)集后(hou)(hou)(hou)(hou)當(dang)天進(jin)行(xing)(xing)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)，儲存(cun)在(zai)(zai)(zai)(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)(ru)(ru)有(you)(you)(you)特殊原因(yin)(yin)(yin)需(xu)(xu)(xu)要(yao)(yao)周期(qi)收(shou)(shou)集標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)， 將(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)及(ji)(ji)時(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)裝后(hou)(hou)(hou)(hou)放(fang)(fang)在(zai)(zai)(zai)(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)-70 ℃條(tiao)件下保存(cun)。避(bi)免(mian)(mian)(mian)反(fan)(fan)(fan)(fan)復凍(dong)融(rong)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)(ke)(ke)(ke)(ke)(ke)保存(cun)48 小(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)(ke)(ke)(ke)(ke)(ke)保存(cun)1 個(ge)月(yue)。-70度(du)(du)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)保存(cun)6 個(ge)月(yue)。部(bu)分(fen)(fen)(fen)激(ji)素(su)(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)需(xu)(xu)(xu)添(tian)加(jia)抑肽(tai)酶(mei)(mei)(mei)(mei)(mei)(mei)。計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)(suan)：以(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)為(wei)(wei)(wei)(wei)橫坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)(zai)(zai)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)上繪出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)(xian)，根(gen)(gen)(gen)(gen)據(ju)(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)(xian)查(cha)出(chu)相(xiang)(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)；再(zai)(zai)乘(cheng)(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)；或(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)(suan)出(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直線(xian)(xian)回(hui)歸方(fang)(fang)程式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)(ru)方(fang)(fang)程式(shi)，計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)(suan)出(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)，再(zai)(zai)乘(cheng)(cheng)(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍數(shu)(shu)(shu)，即(ji)為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)際濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)。 猴(hou)(hou)(hou)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)性(xing)(xing)腫(zhong)(zhong)瘤(liu)壞(huai)(huai)死(si)因(yin)(yin)(yin)子(zi)(zi)-1(sTNF-1)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)疫試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)(he)試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)(he)性(xing)(xing)能1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)性(xing)(xing)回(hui)歸與(yu)預(yu)(yu)期(qi)濃(nong)(nong)(nong)(nong)(nong)(nong)(nong)(nong)度(du)(du)相(xiang)(xiang)關(guan)(guan)系數(shu)(shu)(shu)R值(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)0.92以(yi)(yi)(yi)(yi)(yi)上。2.批(pi)內(nei)(nei)(nei)(nei)與(yu)批(pi)見應(ying)(ying)(ying)(ying)分(fen)(fen)(fen)別(bie)(bie)(bie)小(xiao)于(yu)(yu)(yu)9%和(he)(he)(he)15% 猴(hou)(hou)(hou)可(ke)(ke)(ke)(ke)(ke)(ke)(ke)溶(rong)(rong)(rong)(rong)(rong)(rong)(rong)性(xing)(xing)腫(zhong)(zhong)瘤(liu)壞(huai)(huai)死(si)因(yin)(yin)(yin)子(zi)(zi)-1(sTNF-1)酶(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)免(mian)(mian)(mian)疫試(shi)(shi)(shi)(shi)(shi)劑(ji)盒(he)(he)(he)(he)

實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)原(yuan)(yuan)理   試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)雙(shuang)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)一(yi)(yi)步(bu)夾心(xin)(xin)法(fa)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)(yi)(yi)吸附試(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)（ELISA）。往預先(xian)(xian)(xian)包被γ干(gan)(gan)(gan)擾(rao)(rao)(rao)(rao)素(su)(su)(su)(IFN-γ)捕(bu)獲抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)包被微孔(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，依次(ci)(ci)(ci)加(jia)(jia)入(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)的(de)(de)(de)(de)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)，經過(guo)(guo)(guo)溫育并(bing)徹底(di)洗(xi)(xi)滌。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)TMB顯色(se)(se)，TMB在(zai)(zai)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)的(de)(de)(de)(de)催化(hua)下轉化(hua)成(cheng)藍(lan)色(se)(se)，并(bing)在(zai)(zai)酸的(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)下轉化(hua)成(cheng)＊終(zhong)的(de)(de)(de)(de)黃色(se)(se)。顏(yan)色(se)(se)的(de)(de)(de)(de)深淺(qian)和(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)γ干(gan)(gan)(gan)擾(rao)(rao)(rao)(rao)素(su)(su)(su)(IFN-γ)呈正相(xiang)關。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)在(zai)(zai)450nm 波長(chang)下測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)吸光(guang)度(du)(du)(du)(du)(du)（OD 值(zhi)(zhi)(zhi)(zhi)(zhi)），計(ji)(ji)算(suan)(suan)(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)。保(bao)存(cun)條(tiao)件及(ji)(ji)有(you)效(xiao)(xiao)期(qi)1.本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)體(ti)(ti)(ti)(ti)(ti)(ti)外定(ding)(ding)量(liang)(liang)(liang)(liang)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)血(xue)清(qing)、血(xue)漿(jiang)、組織勻(yun)(yun)漿(jiang)及(ji)(ji)相(xiang)關液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)中(zhong)(zhong)γ干(gan)(gan)(gan)擾(rao)(rao)(rao)(rao)素(su)(su)(su)(IFN-γ)的(de)(de)(de)(de)含量(liang)(liang)(liang)(liang)；2.試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)保(bao)存(cun)：2-8℃；3.有(you)效(xiao)(xiao)期(qi)：6個月(yue)。猴(hou)(hou)γ干(gan)(gan)(gan)擾(rao)(rao)(rao)(rao)素(su)(su)(su)(IFN-γ)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)采(cai)集(ji)及(ji)(ji)保(bao)存(cun)1.血(xue)清(qing)：全血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)請(qing)于(yu)(yu)室溫放置(zhi)2小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)(huo)(huo)(huo)4℃過(guo)(guo)(guo)一(yi)(yi)晚后(hou)(hou)(hou)(hou)(hou)于(yu)(yu)1000 x g離心(xin)(xin)20分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，取(qu)(qu)(qu)上(shang)清(qing)即(ji)可檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放于(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)存(cun)，但(dan)(dan)應(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)反(fan)(fan)(fan)復(fu)凍(dong)融(rong)。 2.血(xue)漿(jiang)：可用(yong)(yong)(yong)(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)(huo)(huo)肝素(su)(su)(su)作(zuo)(zuo)(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)抗(kang)(kang)(kang)凝(ning)劑(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)采(cai)集(ji)后(hou)(hou)(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)于(yu)(yu)2 - 8° C 1000 x g離心(xin)(xin)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，或(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放于(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)存(cun)，但(dan)(dan)應(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)反(fan)(fan)(fan)復(fu)凍(dong)融(rong)。 3.細胞培(pei)養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)上(shang)清(qing)或(huo)(huo)(huo)(huo)(huo)(huo)其它生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)：1000 x g離心(xin)(xin)20分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，取(qu)(qu)(qu)上(shang)清(qing)即(ji)可檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放于(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)存(cun)，但(dan)(dan)應(ying)(ying)(ying)(ying)(ying)(ying)避(bi)(bi)免(mian)(mian)反(fan)(fan)(fan)復(fu)凍(dong)融(rong)。 (注(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)溶(rong)(rong)血(xue)會影響(xiang)**檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)果，因(yin)此(ci)(ci)(ci)溶(rong)(rong)血(xue)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)不(bu)(bu)宜進行(xing)(xing)此(ci)(ci)(ci)項(xiang)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)。) 猴(hou)(hou)γ干(gan)(gan)(gan)擾(rao)(rao)(rao)(rao)素(su)(su)(su)(IFN-γ)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)   標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)則：首先(xian)(xian)(xian)通過(guo)(guo)(guo)文獻(xian)檢(jian)(jian)索(suo)的(de)(de)(de)(de)方(fang)式了解待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)大致含量(liang)(liang)(liang)(liang)，確(que)定(ding)(ding)適當的(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)。只(zhi)有(you)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)的(de)(de)(de)(de)范圍內(nei)(nei)(nei)(nei)，檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)結(jie)(jie)果才是準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)的(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)過(guo)(guo)(guo)程中(zhong)(zhong)，應(ying)(ying)(ying)(ying)(ying)(ying)做(zuo)好(hao)詳細的(de)(de)(de)(de)記(ji)(ji)(ji)(ji)錄(lu)。計(ji)(ji)算(suan)(suan)(suan)濃(nong)(nong)度(du)(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)應(ying)(ying)(ying)(ying)(ying)(ying)再乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)“N”。    標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)的(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)則：2瓶(ping)，每(mei)(mei)(mei)瓶(ping)臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)1ml，蓋好(hao)后(hou)(hou)(hou)(hou)(hou)靜置(zhi)10分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)上(shang)，然后(hou)(hou)(hou)(hou)(hou)反(fan)(fan)(fan)復(fu)顛倒/搓動(dong)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)助溶(rong)(rong)解，其濃(nong)(nong)度(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)300 pg/ml，做(zuo)系列倍(bei)(bei)比(bi)(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)(fen)(fen)別稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)直接作(zuo)(zuo)(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)度(du)(du)(du)(du)(du)0 pg/ml，臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。 如配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)：取(qu)(qu)(qu)0.5ml（不(bu)(bu)要(yao)少于(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)上(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)加(jia)(jia)入(ru)含0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)Eppendorf管中(zhong)(zhong)，混(hun)(hun)(hun)(hun)勻(yun)(yun)即(ji)可，其余濃(nong)(nong)度(du)(du)(du)(du)(du)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)此(ci)(ci)(ci)類推。    生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)則：臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)據(ju)預先(xian)(xian)(xian)計(ji)(ji)算(suan)(suan)(suan)好(hao)的(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)(ci)實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)所需(xu)(xu)(xu)的(de)(de)(de)(de)總(zong)量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)(shi)際(ji)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)多配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如10μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)加(jia)(jia)990μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)比(bi)(bi)例(li)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混(hun)(hun)(hun)(hun)勻(yun)(yun)，在(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。    辣(la)根(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親和(he)(he)素(su)(su)(su)的(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)(yuan)則：臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)辣(la)根(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親和(he)(he)素(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)前(qian)(qian)(qian)根(gen)據(ju)預先(xian)(xian)(xian)計(ji)(ji)算(suan)(suan)(suan)好(hao)的(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)(ci)實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)所需(xu)(xu)(xu)的(de)(de)(de)(de)總(zong)量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)(shi)(shi)(shi)際(ji)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)多配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如10μl辣(la)根(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親和(he)(he)素(su)(su)(su)加(jia)(jia)990μl辣(la)根(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親和(he)(he)素(su)(su)(su)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)比(bi)(bi)例(li)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混(hun)(hun)(hun)(hun)勻(yun)(yun)，在(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)。 猴(hou)(hou)γ干(gan)(gan)(gan)擾(rao)(rao)(rao)(rao)素(su)(su)(su)(IFN-γ)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)操(cao)作(zuo)(zuo)(zuo)(zuo)(zuo)步(bu)驟(zou)   實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)開始前(qian)(qian)(qian)，請(qing)提(ti)前(qian)(qian)(qian)配(pei)(pei)(pei)(pei)(pei)置(zhi)好(hao)所有(you)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)或(huo)(huo)(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，均(jun)需(xu)(xu)(xu)混(hun)(hun)(hun)(hun)勻(yun)(yun)，混(hun)(hun)(hun)(hun)勻(yun)(yun)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盡(jin)(jin)量(liang)(liang)(liang)(liang)避(bi)(bi)免(mian)(mian)起(qi)泡(pao)(pao)。每(mei)(mei)(mei)次(ci)(ci)(ci)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)都(dou)應(ying)(ying)(ying)(ying)(ying)(ying)該做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)。如樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)過(guo)(guo)(guo)高(gao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)進行(xing)(xing)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)(yi)(yi)(yi)使(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)符合試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)的(de)(de)(de)(de)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)范圍。 1.加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)別設空白孔(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)、待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)。空白孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)100μl，余孔(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)別加(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)或(huo)(huo)(huo)(huo)(huo)(huo)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)100μl，注(zhu)意不(bu)(bu)要(yao)有(you)氣(qi)泡(pao)(pao)，加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)加(jia)(jia)于(yu)(yu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)底(di)部，盡(jin)(jin)量(liang)(liang)(liang)(liang)不(bu)(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)壁，輕(qing)(qing)輕(qing)(qing)晃動(dong)混(hun)(hun)(hun)(hun)勻(yun)(yun)，酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)上(shang)蓋或(huo)(huo)(huo)(huo)(huo)(huo)覆膜，37℃反(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 為(wei)(wei)(wei)(wei)保(bao)證(zheng)實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)結(jie)(jie)果有(you)效(xiao)(xiao)性(xing)，每(mei)(mei)(mei)次(ci)(ci)(ci)實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)請(qing)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)溶(rong)(rong)液(ye)(ye)(ye)(ye)。 2.棄(qi)去(qu)液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干(gan)(gan)(gan)，不(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)洗(xi)(xi)滌。每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye) 100μl（取(qu)(qu)(qu)1μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)加(jia)(jia)99μl生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)比(bi)(bi)例(li)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)輕(qing)(qing)混(hun)(hun)(hun)(hun)勻(yun)(yun)，在(zai)(zai)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)(qian)一(yi)(yi)小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。 3.溫育60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)(hou)，棄(qi)去(qu)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干(gan)(gan)(gan)，洗(xi)(xi)板(ban)(ban)(ban)3次(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)浸泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)干(gan)(gan)(gan)。4.每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)辣(la)根(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親和(he)(he)素(su)(su)(su)工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)（同生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。5.溫育60分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)(hou)，棄(qi)去(qu)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)，甩(shuai)(shuai)干(gan)(gan)(gan)，洗(xi)(xi)板(ban)(ban)(ban)5次(ci)(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)(ci)浸泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)干(gan)(gan)(gan)。6.依序(xu)每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)90μl，37℃避(bi)(bi)光(guang)顯色(se)(se)（30分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)，此(ci)(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)肉眼可見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)的(de)(de)(de)(de)前(qian)(qian)(qian)3-4孔(kong)(kong)(kong)(kong)(kong)有(you)明顯的(de)(de)(de)(de)梯(ti)度(du)(du)(du)(du)(du)藍(lan)色(se)(se)，后(hou)(hou)(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)梯(ti)度(du)(du)(du)(du)(du)不(bu)(bu)明顯，即(ji)可終(zhong)止）。7.依序(xu)每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)終(zhong)止溶(rong)(rong)液(ye)(ye)(ye)(ye)50μl，終(zhong)止反(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)（此(ci)(ci)(ci)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)藍(lan)色(se)(se)立轉黃色(se)(se)）。終(zhong)止液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)加(jia)(jia)入(ru)順序(xu)應(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)(jin)量(liang)(liang)(liang)(liang)與(yu)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)加(jia)(jia)入(ru)順序(xu)相(xiang)同。為(wei)(wei)(wei)(wei)了保(bao)證(zheng)實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)結(jie)(jie)果的(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)，底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)反(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)間到后(hou)(hou)(hou)(hou)(hou)應(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)(jin)快加(jia)(jia)入(ru)終(zhong)止液(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)儀(yi)在(zai)(zai)450nm波長(chang)依序(xu)測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)(liang)各孔(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)光(guang)密(mi)度(du)(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)(zhi)(zhi)）。 在(zai)(zai)加(jia)(jia)終(zhong)止液(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)內(nei)(nei)(nei)(nei)進行(xing)(xing)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)。猴(hou)(hou)γ干(gan)(gan)(gan)擾(rao)(rao)(rao)(rao)素(su)(su)(su)(IFN-γ)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)注(zhu)意事項(xiang)1.用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶在(zai)(zai)初次(ci)(ci)(ci)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)(ying)(ying)將(jiang)(jiang)各種試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)管離心(xin)(xin)數(shu)(shu)分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)集(ji)中(zhong)(zhong)到管底(di)。2.每(mei)(mei)(mei)次(ci)(ci)(ci)實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)留(liu)一(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)作(zuo)(zuo)(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)空白調零(ling)孔(kong)(kong)(kong)(kong)(kong)，該孔(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)任何試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)，只(zhi)是**加(jia)(jia)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)溶(rong)(rong)液(ye)(ye)(ye)(ye)及(ji)(ji)2N H2SO4。測(ce)(ce)(ce)(ce)(ce)(ce)量(liang)(liang)(liang)(liang)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)先(xian)(xian)(xian)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)此(ci)(ci)(ci)孔(kong)(kong)(kong)(kong)(kong)調OD值(zhi)(zhi)(zhi)(zhi)(zhi)至(zhi)零(ling)。 3.為(wei)(wei)(wei)(wei)防止樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)蒸(zheng)發，試(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)反(fan)(fan)(fan)應(ying)(ying)(ying)(ying)(ying)(ying)板(ban)(ban)(ban)放于(yu)(yu)鋪有(you)濕布的(de)(de)(de)(de)密(mi)閉盒(he)(he)(he)(he)(he)內(nei)(nei)(nei)(nei)，酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)加(jia)(jia)上(shang)蓋或(huo)(huo)(huo)(huo)(huo)(huo)覆膜。 4.未使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)或(huo)(huo)(huo)(huo)(huo)(huo)者試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)，請(qing)于(yu)(yu)2-8℃保(bao)存(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)、辣(la)根(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親和(he)(he)素(su)(su)(su)工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)請(qing)依據(ju)所需(xu)(xu)(xu)的(de)(de)(de)(de)量(liang)(liang)(liang)(liang)配(pei)(pei)(pei)(pei)(pei)置(zhi)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。請(qing)勿重(zhong)復(fu)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)過(guo)(guo)(guo)的(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)、生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)素(su)(su)(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)(ti)工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)(huo)(huo)、辣(la)根(gen)過(guo)(guo)(guo)氧(yang)(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親和(he)(he)素(su)(su)(su)工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)。 5.建議(yi)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)均(jun)設雙(shuang)孔(kong)(kong)(kong)(kong)(kong)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)，以(yi)(yi)(yi)(yi)(yi)(yi)(yi)保(bao)證(zheng)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)果的(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)確(que)性(xing)。 猴(hou)(hou)γ干(gan)(gan)(gan)擾(rao)(rao)(rao)(rao)素(su)(su)(su)(IFN-γ)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)洗(xi)(xi)板(ban)(ban)(ban)方(fang)法(fa)手工(gong)(gong)洗(xi)(xi)板(ban)(ban)(ban)方(fang)法(fa)：吸去(qu)（不(bu)(bu)可觸及(ji)(ji)板(ban)(ban)(ban)壁）或(huo)(huo)(huo)(huo)(huo)(huo)甩(shuai)(shuai)掉酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)內(nei)(nei)(nei)(nei)的(de)(de)(de)(de)液(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)；在(zai)(zai)實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)臺上(shang)鋪墊幾(ji)層吸水紙，酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)朝下用(yong)(yong)(yong)(yong)(yong)(yong)(yong)力拍(pai)幾(ji)次(ci)(ci)(ci)；將(jiang)(jiang)推薦的(de)(de)(de)(de)洗(xi)(xi)滌緩沖液(ye)(ye)(ye)(ye)至(zhi)少0.3ml注(zhu)入(ru)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)，浸泡(pao)(pao)1-2分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)。根(gen)據(ju)需(xu)(xu)(xu)要(yao)，重(zhong)復(fu)此(ci)(ci)(ci)過(guo)(guo)(guo)程數(shu)(shu)次(ci)(ci)(ci)。 自動(dong)洗(xi)(xi)板(ban)(ban)(ban)：如果有(you)自動(dong)洗(xi)(xi)板(ban)(ban)(ban)機，應(ying)(ying)(ying)(ying)(ying)(ying)在(zai)(zai)熟(shu)練(lian)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)(hou)(hou)再用(yong)(yong)(yong)(yong)(yong)(yong)(yong)到正式實(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)過(guo)(guo)(guo)程中(zhong)(zhong)。 計(ji)(ji)算(suan)(suan)(suan)   以(yi)(yi)(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)橫(heng)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普通坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)半對數(shu)(shu)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)繪出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)，根(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)查(cha)出(chu)(chu)(chu)相(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)；再乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)；或(huo)(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)(zhi)(zhi)計(ji)(ji)算(suan)(suan)(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)的(de)(de)(de)(de)直線(xian)(xian)(xian)(xian)回歸(gui)方(fang)程式，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)代(dai)(dai)入(ru)方(fang)程式，計(ji)(ji)算(suan)(suan)(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)，再乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)，即(ji)為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)實(shi)(shi)(shi)(shi)際(ji)濃(nong)(nong)度(du)(du)(du)(du)(du)。 注(zhu)意事項(xiang)1.當混(hun)(hun)(hun)(hun)合蛋白溶(rong)(rong)液(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)(ying)盡(jin)(jin)量(liang)(liang)(liang)(liang)輕(qing)(qing)緩，避(bi)(bi)免(mian)(mian)起(qi)泡(pao)(pao)。2.洗(xi)(xi)滌過(guo)(guo)(guo)程非(fei)常重(zhong)要(yao)，不(bu)(bu)充(chong)分(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)洗(xi)(xi)滌易造成(cheng)假陽性(xing)。3.一(yi)(yi)次(ci)(ci)(ci)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)間**控制(zhi)(zhi)(zhi)(zhi)(zhi)在(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)，如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)(shu)量(liang)(liang)(liang)(liang)多，推薦使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)排槍加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4.請(qing)每(mei)(mei)(mei)次(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)的(de)(de)(de)(de)同時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)做(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)，做(zuo)復(fu)孔(kong)(kong)(kong)(kong)(kong)。5.如標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中(zhong)(zhong)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質含量(liang)(liang)(liang)(liang)過(guo)(guo)(guo)高(gao)，請(qing)先(xian)(xian)(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)再測(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)，計(ji)(ji)算(suan)(suan)(suan)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)請(qing)乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)。6.在(zai)(zai)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)、檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)溶(rong)(rong)液(ye)(ye)(ye)(ye)工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，請(qing)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)相(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)(zhi)，不(bu)(bu)能混(hun)(hun)(hun)(hun)淆。7.底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)請(qing)避(bi)(bi)光(guang)保(bao)存(cun)。 8.不(bu)(bu)要(yao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)其它生(sheng)(sheng)(sheng)產廠(chang)家的(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)替換試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)中(zhong)(zhong)的(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)。 注(zhu)意事項(xiang)：收(shou)(shou)集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)前(qian)(qian)(qian)必須清(qing)楚要(yao)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)成(cheng)份是否足夠穩定(ding)(ding)。對收(shou)(shou)集(ji)后(hou)(hou)(hou)(hou)(hou)當天進行(xing)(xing)檢(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)，儲存(cun)在(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如有(you)特殊原(yuan)(yuan)因(yin)需(xu)(xu)(xu)要(yao)周期(qi)收(shou)(shou)集(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)， 將(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)及(ji)(ji)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)后(hou)(hou)(hou)(hou)(hou)放在(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)(huo)(huo)-70 ℃條(tiao)件下保(bao)存(cun)。避(bi)(bi)免(mian)(mian)反(fan)(fan)(fan)復(fu)凍(dong)融(rong)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)2 -8 ℃可保(bao)存(cun)48 小(xiao)(xiao)時(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)，-20 ℃可保(bao)存(cun)1 個月(yue)。-70度(du)(du)(du)(du)(du)可保(bao)存(cun)6 個月(yue)。部分(fen)(fen)(fen)(fen)(fen)(fen)激素(su)(su)(su)類標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)需(xu)(xu)(xu)添加(jia)(jia)抑肽酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)。計(ji)(ji)算(suan)(suan)(suan)：以(yi)(yi)(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)為(wei)(wei)(wei)(wei)橫(heng)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)縱坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)坐(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上(shang)繪出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)，根(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)查(cha)出(chu)(chu)(chu)相(xiang)應(ying)(ying)(ying)(ying)(ying)(ying)的(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)；再乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)；或(huo)(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)(zhi)(zhi)計(ji)(ji)算(suan)(suan)(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)(xian)(xian)的(de)(de)(de)(de)直線(xian)(xian)(xian)(xian)回歸(gui)方(fang)程式，將(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)(zhi)(zhi)代(dai)(dai)入(ru)方(fang)程式，計(ji)(ji)算(suan)(suan)(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)，再乘(cheng)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)，即(ji)為(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)實(shi)(shi)(shi)(shi)際(ji)濃(nong)(nong)度(du)(du)(du)(du)(du)。 猴(hou)(hou)γ干(gan)(gan)(gan)擾(rao)(rao)(rao)(rao)素(su)(su)(su)(IFN-γ)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)性(xing)能1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)線(xian)(xian)(xian)(xian)性(xing)回歸(gui)與(yu)預期(qi)濃(nong)(nong)度(du)(du)(du)(du)(du)相(xiang)關系數(shu)(shu)R值(zhi)(zhi)(zhi)(zhi)(zhi)為(wei)(wei)(wei)(wei)0.92以(yi)(yi)(yi)(yi)(yi)(yi)(yi)上(shang)。2.批內(nei)(nei)(nei)(nei)與(yu)批見應(ying)(ying)(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)(fen)(fen)別小(xiao)(xiao)于(yu)(yu)9%和(he)(he)15% 猴(hou)(hou)γ干(gan)(gan)(gan)擾(rao)(rao)(rao)(rao)素(su)(su)(su)(IFN-γ)酶(mei)(mei)(mei)(mei)(mei)(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)(yi)(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)

實(shi)驗(yan)(yan)(yan)原(yuan)理   試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)采(cai)用(yong)(yong)(yong)(yong)雙(shuang)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)一(yi)(yi)(yi)步夾心(xin)法(fa)酶(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)吸附試(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)（ELISA）。往預(yu)先(xian)包被(bei)載(zai)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)C3(ApoC3)捕獲抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)包被(bei)微(wei)孔(kong)(kong)(kong)(kong)(kong)中(zhong)，依(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)，經過溫育(yu)并徹底(di)(di)(di)洗(xi)(xi)滌(di)(di)。用(yong)(yong)(yong)(yong)底(di)(di)(di)物(wu)(wu)(wu)TMB顯(xian)色(se)，TMB在(zai)(zai)(zai)過氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)催化(hua)(hua)(hua)(hua)下(xia)(xia)轉(zhuan)化(hua)(hua)(hua)(hua)成(cheng)(cheng)藍(lan)色(se)，并在(zai)(zai)(zai)酸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作用(yong)(yong)(yong)(yong)下(xia)(xia)轉(zhuan)化(hua)(hua)(hua)(hua)成(cheng)(cheng)＊終(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)黃色(se)。顏(yan)色(se)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)深淺和(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)載(zai)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)C3(ApoC3)呈正相(xiang)關(guan)。用(yong)(yong)(yong)(yong)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)在(zai)(zai)(zai)450nm 波長(chang)下(xia)(xia)測(ce)(ce)定(ding)(ding)吸光(guang)度(du)(du)(du)(du)(du)(du)（OD 值(zhi)(zhi)(zhi)），計(ji)(ji)算(suan)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)。保(bao)(bao)(bao)存(cun)(cun)條件(jian)及(ji)(ji)有(you)(you)效期(qi)1.本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)用(yong)(yong)(yong)(yong)于(yu)(yu)體(ti)(ti)(ti)(ti)(ti)外定(ding)(ding)量(liang)(liang)(liang)檢(jian)(jian)(jian)測(ce)(ce)血清(qing)、血漿、組織(zhi)勻(yun)(yun)(yun)漿及(ji)(ji)相(xiang)關(guan)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)中(zhong)載(zai)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)C3(ApoC3)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含量(liang)(liang)(liang)；2.試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)保(bao)(bao)(bao)存(cun)(cun)：2-8℃；3.有(you)(you)效期(qi)：6個月。猴(hou)(hou)載(zai)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)C3(ApoC3)酶(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)采(cai)集(ji)(ji)(ji)及(ji)(ji)保(bao)(bao)(bao)存(cun)(cun)1.血清(qing)：全血標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)請于(yu)(yu)室溫放(fang)置2小(xiao)時(shi)(shi)(shi)(shi)(shi)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)4℃過一(yi)(yi)(yi)晚(wan)后(hou)(hou)(hou)(hou)(hou)于(yu)(yu)1000 x g離心(xin)20分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，取上(shang)清(qing)即(ji)(ji)(ji)可(ke)(ke)(ke)檢(jian)(jian)(jian)測(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)存(cun)(cun)，但應(ying)(ying)(ying)(ying)避免(mian)(mian)反(fan)(fan)復(fu)(fu)(fu)凍融(rong)(rong)。 2.血漿：可(ke)(ke)(ke)用(yong)(yong)(yong)(yong)EDTA或(huo)(huo)(huo)(huo)(huo)(huo)(huo)肝(gan)素(su)作為(wei)抗(kang)(kang)(kang)凝劑(ji)(ji)(ji)(ji)(ji)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)采(cai)集(ji)(ji)(ji)后(hou)(hou)(hou)(hou)(hou)30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)于(yu)(yu)2 - 8° C 1000 x g離心(xin)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，或(huo)(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)存(cun)(cun)，但應(ying)(ying)(ying)(ying)避免(mian)(mian)反(fan)(fan)復(fu)(fu)(fu)凍融(rong)(rong)。 3.細胞(bao)培養(yang)物(wu)(wu)(wu)上(shang)清(qing)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)其(qi)它(ta)(ta)生(sheng)物(wu)(wu)(wu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)：1000 x g離心(xin)20分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，取上(shang)清(qing)即(ji)(ji)(ji)可(ke)(ke)(ke)檢(jian)(jian)(jian)測(ce)(ce)，或(huo)(huo)(huo)(huo)(huo)(huo)(huo)將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)放(fang)于(yu)(yu)-20℃或(huo)(huo)(huo)(huo)(huo)(huo)(huo)-80℃保(bao)(bao)(bao)存(cun)(cun)，但應(ying)(ying)(ying)(ying)避免(mian)(mian)反(fan)(fan)復(fu)(fu)(fu)凍融(rong)(rong)。 (注(zhu)(zhu)：標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)溶血會影響**檢(jian)(jian)(jian)測(ce)(ce)結(jie)果(guo)，因此(ci)溶血標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)不(bu)(bu)(bu)宜進行此(ci)項檢(jian)(jian)(jian)測(ce)(ce)。) 猴(hou)(hou)載(zai)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)C3(ApoC3)酶(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)   標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則：首先(xian)通過文獻檢(jian)(jian)(jian)索的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)式(shi)了解待(dai)測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大(da)致含量(liang)(liang)(liang)，確(que)(que)定(ding)(ding)適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)。只(zhi)有(you)(you)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范(fan)圍內(nei)(nei)(nei)(nei)(nei)，檢(jian)(jian)(jian)測(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)結(jie)果(guo)才是準(zhun)(zhun)(zhun)(zhun)確(que)(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過程(cheng)(cheng)中(zhong)，應(ying)(ying)(ying)(ying)做(zuo)(zuo)好(hao)詳細的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)記(ji)(ji)(ji)(ji)錄。計(ji)(ji)算(suan)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)時(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)了“N”倍(bei)(bei)，標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)應(ying)(ying)(ying)(ying)再(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)“N”。    標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則：2瓶，每(mei)(mei)(mei)瓶臨(lin)用(yong)(yong)(yong)(yong)前以(yi)(yi)(yi)(yi)(yi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)至(zhi)1ml，蓋好(hao)后(hou)(hou)(hou)(hou)(hou)靜(jing)置10分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)上(shang)，然(ran)后(hou)(hou)(hou)(hou)(hou)反(fan)(fan)復(fu)(fu)(fu)顛(dian)倒(dao)/搓(cuo)動(dong)以(yi)(yi)(yi)(yi)(yi)助溶解，其(qi)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)300 pg/ml，做(zuo)(zuo)系列倍(bei)(bei)比(bi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)，分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)300 pg/ml，150 pg/ml，75 pg/ml，37.5 pg/ml，18.5 pg/ml，9 pg/ml，4.5 pg/ml，樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)直(zhi)(zhi)接作為(wei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)0 pg/ml，臨(lin)用(yong)(yong)(yong)(yong)前15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)。 如(ru)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)150 pg/ml標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)：取0.5ml（不(bu)(bu)(bu)要少(shao)于(yu)(yu)0.5ml）300 pg/ml的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)上(shang)述標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)含0.5ml樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Eppendorf管中(zhong)，混(hun)(hun)勻(yun)(yun)(yun)即(ji)(ji)(ji)可(ke)(ke)(ke)，其(qi)余(yu)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)以(yi)(yi)(yi)(yi)(yi)此(ci)類(lei)推(tui)。    生(sheng)物(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則：臨(lin)用(yong)(yong)(yong)(yong)前以(yi)(yi)(yi)(yi)(yi)生(sheng)物(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)前根(gen)(gen)(gen)據(ju)預(yu)先(xian)計(ji)(ji)算(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)實(shi)驗(yan)(yan)(yan)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)(zong)量(liang)(liang)(liang)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)多(duo)(duo)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)10μl生(sheng)物(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl生(sheng)物(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)(qing)輕(qing)(qing)(qing)(qing)混(hun)(hun)勻(yun)(yun)(yun)，在(zai)(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)前一(yi)(yi)(yi)小(xiao)時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)。    辣(la)(la)(la)根(gen)(gen)(gen)過氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)原(yuan)則：臨(lin)用(yong)(yong)(yong)(yong)前以(yi)(yi)(yi)(yi)(yi)辣(la)(la)(la)根(gen)(gen)(gen)過氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)，稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)前根(gen)(gen)(gen)據(ju)預(yu)先(xian)計(ji)(ji)算(suan)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)每(mei)(mei)(mei)次(ci)(ci)實(shi)驗(yan)(yan)(yan)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)總(zong)(zong)量(liang)(liang)(liang)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)（每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)100μl），實(shi)際配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)多(duo)(duo)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)0.1-0.2ml。如(ru)10μl辣(la)(la)(la)根(gen)(gen)(gen)過氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)990μl辣(la)(la)(la)根(gen)(gen)(gen)過氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)(qing)輕(qing)(qing)(qing)(qing)混(hun)(hun)勻(yun)(yun)(yun)，在(zai)(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)前一(yi)(yi)(yi)小(xiao)時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)。 猴(hou)(hou)載(zai)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)C3(ApoC3)酶(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)操作步驟   實(shi)驗(yan)(yan)(yan)開始前，請提前配(pei)(pei)(pei)置好(hao)所有(you)(you)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)時(shi)(shi)(shi)(shi)(shi)，均需混(hun)(hun)勻(yun)(yun)(yun)，混(hun)(hun)勻(yun)(yun)(yun)時(shi)(shi)(shi)(shi)(shi)盡(jin)量(liang)(liang)(liang)避免(mian)(mian)起泡。每(mei)(mei)(mei)次(ci)(ci)檢(jian)(jian)(jian)測(ce)(ce)都(dou)應(ying)(ying)(ying)(ying)該(gai)做(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)。如(ru)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)過高(gao)時(shi)(shi)(shi)(shi)(shi)，用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)進行稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)，以(yi)(yi)(yi)(yi)(yi)使(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)符合試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)范(fan)圍。 1.加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別設(she)空白(bai)孔(kong)(kong)(kong)(kong)(kong)、標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)孔(kong)(kong)(kong)(kong)(kong)、待(dai)測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)。空白(bai)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)100μl，余(yu)孔(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)待(dai)測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)100μl，注(zhu)(zhu)意(yi)不(bu)(bu)(bu)要有(you)(you)氣泡，加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)于(yu)(yu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)底(di)(di)(di)部，盡(jin)量(liang)(liang)(liang)不(bu)(bu)(bu)觸及(ji)(ji)孔(kong)(kong)(kong)(kong)(kong)壁(bi)，輕(qing)(qing)(qing)(qing)輕(qing)(qing)(qing)(qing)晃動(dong)混(hun)(hun)勻(yun)(yun)(yun)，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)蓋或(huo)(huo)(huo)(huo)(huo)(huo)(huo)覆(fu)膜，37℃反(fan)(fan)應(ying)(ying)(ying)(ying)120分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。 為(wei)保(bao)(bao)(bao)證實(shi)驗(yan)(yan)(yan)結(jie)果(guo)有(you)(you)效性(xing)，每(mei)(mei)(mei)次(ci)(ci)實(shi)驗(yan)(yan)(yan)請使(shi)(shi)用(yong)(yong)(yong)(yong)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)溶液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 2.棄去(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩(shuai)(shuai)(shuai)干(gan)(gan)，不(bu)(bu)(bu)用(yong)(yong)(yong)(yong)洗(xi)(xi)滌(di)(di)。每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)生(sheng)物(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)作液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye) 100μl（取1μl生(sheng)物(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)99μl生(sheng)物(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)，輕(qing)(qing)(qing)(qing)輕(qing)(qing)(qing)(qing)混(hun)(hun)勻(yun)(yun)(yun)，在(zai)(zai)(zai)使(shi)(shi)用(yong)(yong)(yong)(yong)前一(yi)(yi)(yi)小(xiao)時(shi)(shi)(shi)(shi)(shi)內(nei)(nei)(nei)(nei)(nei)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)），37℃,60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。 3.溫育(yu)60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)(hou)，棄去(qu)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩(shuai)(shuai)(shuai)干(gan)(gan)，洗(xi)(xi)板(ban)(ban)3次(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)浸(jin)泡1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)(shuai)干(gan)(gan)。4.每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)辣(la)(la)(la)根(gen)(gen)(gen)過氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)工(gong)作液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)（同生(sheng)物(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)作液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)） 100μl，37℃，60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。5.溫育(yu)60分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)后(hou)(hou)(hou)(hou)(hou)，棄去(qu)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)，甩(shuai)(shuai)(shuai)干(gan)(gan)，洗(xi)(xi)板(ban)(ban)5次(ci)(ci)，每(mei)(mei)(mei)次(ci)(ci)浸(jin)泡1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，350μl/每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)，甩(shuai)(shuai)(shuai)干(gan)(gan)。6.依(yi)序(xu)每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)(di)(di)物(wu)(wu)(wu)溶液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)90μl，37℃避光(guang)顯(xian)色(se)（30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)，此(ci)時(shi)(shi)(shi)(shi)(shi)肉(rou)眼(yan)可(ke)(ke)(ke)見標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前3-4孔(kong)(kong)(kong)(kong)(kong)有(you)(you)明(ming)顯(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)梯度(du)(du)(du)(du)(du)(du)藍(lan)色(se)，后(hou)(hou)(hou)(hou)(hou)3-4孔(kong)(kong)(kong)(kong)(kong)梯度(du)(du)(du)(du)(du)(du)不(bu)(bu)(bu)明(ming)顯(xian)，即(ji)(ji)(ji)可(ke)(ke)(ke)終(zhong)止(zhi)(zhi)）。7.依(yi)序(xu)每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)(zhi)溶液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)50μl，終(zhong)止(zhi)(zhi)反(fan)(fan)應(ying)(ying)(ying)(ying)（此(ci)時(shi)(shi)(shi)(shi)(shi)藍(lan)色(se)立轉(zhuan)黃色(se)）。終(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)順序(xu)應(ying)(ying)(ying)(ying)盡(jin)量(liang)(liang)(liang)與(yu)底(di)(di)(di)物(wu)(wu)(wu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)順序(xu)相(xiang)同。為(wei)了保(bao)(bao)(bao)證實(shi)驗(yan)(yan)(yan)結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)確(que)(que)性(xing)，底(di)(di)(di)物(wu)(wu)(wu)反(fan)(fan)應(ying)(ying)(ying)(ying)時(shi)(shi)(shi)(shi)(shi)間到(dao)后(hou)(hou)(hou)(hou)(hou)應(ying)(ying)(ying)(ying)盡(jin)快加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)終(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。8.用(yong)(yong)(yong)(yong)酶(mei)(mei)聯(lian)(lian)(lian)儀(yi)在(zai)(zai)(zai)450nm波長(chang)依(yi)序(xu)測(ce)(ce)量(liang)(liang)(liang)各孔(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)密(mi)度(du)(du)(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)）。 在(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)終(zhong)止(zhi)(zhi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)以(yi)(yi)(yi)(yi)(yi)內(nei)(nei)(nei)(nei)(nei)進行檢(jian)(jian)(jian)測(ce)(ce)。猴(hou)(hou)載(zai)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)C3(ApoC3)酶(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)注(zhu)(zhu)意(yi)事項1.用(yong)(yong)(yong)(yong)戶在(zai)(zai)(zai)初次(ci)(ci)使(shi)(shi)用(yong)(yong)(yong)(yong)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)時(shi)(shi)(shi)(shi)(shi)，應(ying)(ying)(ying)(ying)將(jiang)(jiang)(jiang)各種試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)管離心(xin)數(shu)(shu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)，以(yi)(yi)(yi)(yi)(yi)便試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)集(ji)(ji)(ji)中(zhong)到(dao)管底(di)(di)(di)。2.每(mei)(mei)(mei)次(ci)(ci)實(shi)驗(yan)(yan)(yan)留一(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)作為(wei)空白(bai)調(diao)零(ling)孔(kong)(kong)(kong)(kong)(kong)，該(gai)孔(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)任(ren)何(he)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，只(zhi)是**加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)底(di)(di)(di)物(wu)(wu)(wu)溶液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)及(ji)(ji)2N H2SO4。測(ce)(ce)量(liang)(liang)(liang)時(shi)(shi)(shi)(shi)(shi)先(xian)用(yong)(yong)(yong)(yong)此(ci)孔(kong)(kong)(kong)(kong)(kong)調(diao)OD值(zhi)(zhi)(zhi)至(zhi)零(ling)。 3.為(wei)防(fang)止(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)蒸(zheng)發，試(shi)(shi)(shi)(shi)(shi)(shi)驗(yan)(yan)(yan)時(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)(jiang)反(fan)(fan)應(ying)(ying)(ying)(ying)板(ban)(ban)放(fang)于(yu)(yu)鋪(pu)有(you)(you)濕布的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)密(mi)閉盒(he)(he)(he)(he)內(nei)(nei)(nei)(nei)(nei)，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)上(shang)蓋或(huo)(huo)(huo)(huo)(huo)(huo)(huo)覆(fu)膜。 4.未使(shi)(shi)用(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)者(zhe)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，請于(yu)(yu)2-8℃保(bao)(bao)(bao)存(cun)(cun)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)物(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)作液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)、辣(la)(la)(la)根(gen)(gen)(gen)過氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)工(gong)作液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)請依(yi)據(ju)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)配(pei)(pei)(pei)置使(shi)(shi)用(yong)(yong)(yong)(yong)。請勿重(zhong)復(fu)(fu)(fu)使(shi)(shi)用(yong)(yong)(yong)(yong)已稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)過的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、生(sheng)物(wu)(wu)(wu)素(su)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)抗(kang)(kang)(kang)體(ti)(ti)(ti)(ti)(ti)工(gong)作液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)、辣(la)(la)(la)根(gen)(gen)(gen)過氧(yang)(yang)化(hua)(hua)(hua)(hua)物(wu)(wu)(wu)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)記(ji)(ji)(ji)(ji)親(qin)和(he)(he)素(su)工(gong)作液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。 5.建(jian)議檢(jian)(jian)(jian)測(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)時(shi)(shi)(shi)(shi)(shi)均設(she)雙(shuang)孔(kong)(kong)(kong)(kong)(kong)測(ce)(ce)定(ding)(ding)，以(yi)(yi)(yi)(yi)(yi)保(bao)(bao)(bao)證檢(jian)(jian)(jian)測(ce)(ce)結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)確(que)(que)性(xing)。 猴(hou)(hou)載(zai)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)C3(ApoC3)酶(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)洗(xi)(xi)板(ban)(ban)方(fang)法(fa)手工(gong)洗(xi)(xi)板(ban)(ban)方(fang)法(fa)：吸去(qu)（不(bu)(bu)(bu)可(ke)(ke)(ke)觸及(ji)(ji)板(ban)(ban)壁(bi)）或(huo)(huo)(huo)(huo)(huo)(huo)(huo)甩(shuai)(shuai)(shuai)掉(diao)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)內(nei)(nei)(nei)(nei)(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)；在(zai)(zai)(zai)實(shi)驗(yan)(yan)(yan)臺上(shang)鋪(pu)墊幾(ji)層吸水紙(zhi)(zhi)(zhi)，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)朝下(xia)(xia)用(yong)(yong)(yong)(yong)力拍幾(ji)次(ci)(ci)；將(jiang)(jiang)(jiang)推(tui)薦(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)(di)緩(huan)沖液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)至(zhi)少(shao)0.3ml注(zhu)(zhu)入(ru)(ru)(ru)(ru)(ru)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)(nei)(nei)(nei)，浸(jin)泡1-2分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)。根(gen)(gen)(gen)據(ju)需要，重(zhong)復(fu)(fu)(fu)此(ci)過程(cheng)(cheng)數(shu)(shu)次(ci)(ci)。 自動(dong)洗(xi)(xi)板(ban)(ban)：如(ru)果(guo)有(you)(you)自動(dong)洗(xi)(xi)板(ban)(ban)機，應(ying)(ying)(ying)(ying)在(zai)(zai)(zai)熟練使(shi)(shi)用(yong)(yong)(yong)(yong)后(hou)(hou)(hou)(hou)(hou)再(zai)用(yong)(yong)(yong)(yong)到(dao)正式(shi)實(shi)驗(yan)(yan)(yan)過程(cheng)(cheng)中(zhong)。 計(ji)(ji)算(suan)   以(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)橫(heng)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（對數(shu)(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），OD值(zhi)(zhi)(zhi)為(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)（普(pu)通坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)），在(zai)(zai)(zai)半(ban)對數(shu)(shu)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)(zhi)(zhi)上(shang)繪(hui)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)，根(gen)(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)查出(chu)(chu)(chu)相(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)；再(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)；或(huo)(huo)(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)(ji)算(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)(zhi)線(xian)(xian)回歸方(fang)程(cheng)(cheng)式(shi)，將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)(ru)(ru)(ru)(ru)方(fang)程(cheng)(cheng)式(shi)，計(ji)(ji)算(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)，再(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)，即(ji)(ji)(ji)為(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際濃(nong)(nong)度(du)(du)(du)(du)(du)(du)。 注(zhu)(zhu)意(yi)事項1.當混(hun)(hun)合蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)溶液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)盡(jin)量(liang)(liang)(liang)輕(qing)(qing)(qing)(qing)緩(huan)，避免(mian)(mian)起泡。2.洗(xi)(xi)滌(di)(di)過程(cheng)(cheng)非常重(zhong)要，不(bu)(bu)(bu)充分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)洗(xi)(xi)滌(di)(di)易造成(cheng)(cheng)假陽性(xing)。3.一(yi)(yi)(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)間**控制(zhi)(zhi)(zhi)(zhi)在(zai)(zai)(zai)5分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘(zhong)(zhong)(zhong)(zhong)內(nei)(nei)(nei)(nei)(nei)，如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)數(shu)(shu)量(liang)(liang)(liang)多(duo)(duo)，推(tui)薦(jian)使(shi)(shi)用(yong)(yong)(yong)(yong)排(pai)槍加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4.請每(mei)(mei)(mei)次(ci)(ci)測(ce)(ce)定(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi)(shi)(shi)(shi)做(zuo)(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)，做(zuo)(zuo)復(fu)(fu)(fu)孔(kong)(kong)(kong)(kong)(kong)。5.如(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)中(zhong)待(dai)測(ce)(ce)物(wu)(wu)(wu)質含量(liang)(liang)(liang)過高(gao)，請先(xian)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)后(hou)(hou)(hou)(hou)(hou)再(zai)測(ce)(ce)定(ding)(ding)，計(ji)(ji)算(suan)時(shi)(shi)(shi)(shi)(shi)請乘(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)。6.在(zai)(zai)(zai)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)、檢(jian)(jian)(jian)測(ce)(ce)溶液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)工(gong)作液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)時(shi)(shi)(shi)(shi)(shi)，請以(yi)(yi)(yi)(yi)(yi)相(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)制(zhi)(zhi)(zhi)(zhi)，不(bu)(bu)(bu)能(neng)(neng)混(hun)(hun)淆。7.底(di)(di)(di)物(wu)(wu)(wu)請避光(guang)保(bao)(bao)(bao)存(cun)(cun)。 8.不(bu)(bu)(bu)要用(yong)(yong)(yong)(yong)其(qi)它(ta)(ta)生(sheng)產廠家(jia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)替(ti)換試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)中(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)。 注(zhu)(zhu)意(yi)事項：收(shou)集(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)前必(bi)須清(qing)楚(chu)要檢(jian)(jian)(jian)測(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)份是否足夠穩定(ding)(ding)。對收(shou)集(ji)(ji)(ji)后(hou)(hou)(hou)(hou)(hou)當天進行檢(jian)(jian)(jian)測(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)，儲存(cun)(cun)在(zai)(zai)(zai)4 ℃備用(yong)(yong)(yong)(yong)，如(ru)有(you)(you)特殊原(yuan)因需要周期(qi)收(shou)集(ji)(ji)(ji)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)， 將(jiang)(jiang)(jiang)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)及(ji)(ji)時(shi)(shi)(shi)(shi)(shi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)后(hou)(hou)(hou)(hou)(hou)放(fang)在(zai)(zai)(zai)-20 ℃或(huo)(huo)(huo)(huo)(huo)(huo)(huo)-70 ℃條件(jian)下(xia)(xia)保(bao)(bao)(bao)存(cun)(cun)。避免(mian)(mian)反(fan)(fan)復(fu)(fu)(fu)凍融(rong)(rong)。標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)2 -8 ℃可(ke)(ke)(ke)保(bao)(bao)(bao)存(cun)(cun)48 小(xiao)時(shi)(shi)(shi)(shi)(shi)，-20 ℃可(ke)(ke)(ke)保(bao)(bao)(bao)存(cun)(cun)1 個月。-70度(du)(du)(du)(du)(du)(du)可(ke)(ke)(ke)保(bao)(bao)(bao)存(cun)(cun)6 個月。部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)激素(su)類(lei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)需添加(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)(jia)抑肽酶(mei)(mei)。計(ji)(ji)算(suan)：以(yi)(yi)(yi)(yi)(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)為(wei)橫(heng)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為(wei)縱坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，在(zai)(zai)(zai)坐標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙(zhi)(zhi)(zhi)上(shang)繪(hui)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)，根(gen)(gen)(gen)據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)由標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)查出(chu)(chu)(chu)相(xiang)應(ying)(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)；再(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)；或(huo)(huo)(huo)(huo)(huo)(huo)(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)與(yu)OD值(zhi)(zhi)(zhi)計(ji)(ji)算(suan)出(chu)(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)(qu)(qu)線(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)直(zhi)(zhi)線(xian)(xian)回歸方(fang)程(cheng)(cheng)式(shi)，將(jiang)(jiang)(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)代入(ru)(ru)(ru)(ru)(ru)方(fang)程(cheng)(cheng)式(shi)，計(ji)(ji)算(suan)出(chu)(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)，再(zai)乘(cheng)以(yi)(yi)(yi)(yi)(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)(shi)(shi)(shi)(shi)(shi)倍(bei)(bei)數(shu)(shu)，即(ji)(ji)(ji)為(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)際濃(nong)(nong)度(du)(du)(du)(du)(du)(du)。 猴(hou)(hou)載(zai)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)C3(ApoC3)酶(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)性(xing)能(neng)(neng)1.樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)(pin)線(xian)(xian)性(xing)回歸與(yu)預(yu)期(qi)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)相(xiang)關(guan)系數(shu)(shu)R值(zhi)(zhi)(zhi)為(wei)0.92以(yi)(yi)(yi)(yi)(yi)上(shang)。2.批內(nei)(nei)(nei)(nei)(nei)與(yu)批見應(ying)(ying)(ying)(ying)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別小(xiao)于(yu)(yu)9%和(he)(he)15% 猴(hou)(hou)載(zai)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)C3(ApoC3)酶(mei)(mei)聯(lian)(lian)(lian)免(mian)(mian)疫(yi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)