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大(da)(da)鼠(shu)血管內(nei)皮細胞(bao)生(sheng)(sheng)(sheng)長因(yin)子A(VEGF-A)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)文(wen)名稱大(da)(da)鼠(shu)血管內(nei)皮細胞(bao)生(sheng)(sheng)(sheng)長因(yin)子A(VEGF-A)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)規格(ge)96T/48T樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)類型血清、血漿、細胞(bao)上清液(ye)(ye)(ye)(ye)、尿液(ye)(ye)(ye)(ye)、體液(ye)(ye)(ye)(ye)、灌洗(xi)液(ye)(ye)(ye)(ye)等使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)范圍科研(yan)實驗(yan)，不得用(yong)(yong)(yong)(yong)于臨(lin)床診斷(duan)反應(ying)(ying)(ying)時(shi)間(jian)1-5h試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)保(bao)存(cun)2-8℃有(you)(you)(you)(you)效期6個(ge)月貨(huo)(huo)期現貨(huo)(huo)【操(cao)(cao)(cao)作(zuo)(zuo)步(bu)驟】1. 標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)與(yu)(yu)(yu)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：在(zai)(zai)(zai)(zai)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)(ban)(ban)上設(she)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)10孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)(yi)(yi)(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)100μl，然(ran)(ran)后(hou)(hou)(hou)(hou)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)(yi)(yi)(yi)(yi)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻；然(ran)(ran)后(hou)(hou)(hou)(hou)從第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)(yi)(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)二孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)各(ge)(ge)取(qu)100μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，再(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)三、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻；然(ran)(ran)后(hou)(hou)(hou)(hou)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)三孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)四(si)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)先(xian)(xian)各(ge)(ge)取(qu)50μl棄(qi)掉，再(zai)(zai)各(ge)(ge)取(qu)50μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，再(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)50ul，混(hun)(hun)(hun)勻；混(hun)(hun)(hun)勻后(hou)(hou)(hou)(hou)從第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)五(wu)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)六孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)各(ge)(ge)取(qu)50μl分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)七(qi)(qi)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，再(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)七(qi)(qi)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻后(hou)(hou)(hou)(hou)從第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)七(qi)(qi)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)八孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)取(qu)50μl加(jia)(jia)(jia)(jia)(jia)到(dao)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)九(jiu)(jiu)、第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，再(zai)(zai)在(zai)(zai)(zai)(zai)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)九(jiu)(jiu)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)加(jia)(jia)(jia)(jia)(jia)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)50μl，混(hun)(hun)(hun)勻后(hou)(hou)(hou)(hou)從第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)九(jiu)(jiu)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)十孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)各(ge)(ge)取(qu)50μl棄(qi)掉。（稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)后(hou)(hou)(hou)(hou)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)都為50μl，濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)為3 μg/L，2μg/L ，1.0μg/L，0.5μg/L，0.25μg/L）。2. 加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)：分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)設(she)空(kong)(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)（空(kong)(kong)白對照孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)及(ji)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)，其(qi)余各(ge)(ge)步(bu)操(cao)(cao)(cao)作(zuo)(zuo)相(xiang)同(tong)）、待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。在(zai)(zai)(zai)(zai)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)(ban)(ban)上待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)先(xian)(xian)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)40μl，然(ran)(ran)后(hou)(hou)(hou)(hou)再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)10μl（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)＊終(zhong)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)度(du)(du)(du)(du)為5倍(bei)(bei)(bei)）。加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)加(jia)(jia)(jia)(jia)(jia)于酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)底部(bu)，盡(jin)量(liang)(liang)不觸及(ji)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)壁，輕(qing)輕(qing)晃動混(hun)(hun)(hun)勻。3. 溫(wen)育(yu)：用(yong)(yong)(yong)(yong)封(feng)(feng)板(ban)(ban)(ban)(ban)膜(mo)(mo)封(feng)(feng)板(ban)(ban)(ban)(ban)后(hou)(hou)(hou)(hou)置37℃溫(wen)育(yu)30分(fen)(fen)(fen)鐘(zhong)。4. 配液(ye)(ye)(ye)(ye)：將(jiang)30（48T的(de)(de)(de)(de)(de)20倍(bei)(bei)(bei)）倍(bei)(bei)(bei)濃(nong)(nong)(nong)(nong)縮洗(xi)滌液(ye)(ye)(ye)(ye)用(yong)(yong)(yong)(yong)蒸餾水(shui)(shui)30（48T的(de)(de)(de)(de)(de)20倍(bei)(bei)(bei)）倍(bei)(bei)(bei)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)后(hou)(hou)(hou)(hou)備用(yong)(yong)(yong)(yong)。5. 洗(xi)滌：小(xiao)心(xin)揭掉封(feng)(feng)板(ban)(ban)(ban)(ban)膜(mo)(mo)，棄(qi)去(qu)(qu)液(ye)(ye)(ye)(ye)體，甩干，每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)滿洗(xi)滌液(ye)(ye)(ye)(ye)，靜置30秒后(hou)(hou)(hou)(hou)棄(qi)去(qu)(qu)，如(ru)(ru)此重(zhong)(zhong)復(fu)5次(ci)(ci)，拍干。6. 加(jia)(jia)(jia)(jia)(jia)酶(mei)(mei)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)50μl，空(kong)(kong)白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)除外。7. 溫(wen)育(yu)：操(cao)(cao)(cao)作(zuo)(zuo)同(tong)3。8. 洗(xi)滌：操(cao)(cao)(cao)作(zuo)(zuo)同(tong)5。9. 顯(xian)(xian)(xian)色(se)(se)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)(xian)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)顯(xian)(xian)(xian)色(se)(se)劑(ji)(ji)(ji)(ji)(ji)A50μl，再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)顯(xian)(xian)(xian)色(se)(se)劑(ji)(ji)(ji)(ji)(ji)B50μl，輕(qing)輕(qing)震蕩混(hun)(hun)(hun)勻，37℃避(bi)(bi)光(guang)顯(xian)(xian)(xian)色(se)(se)15分(fen)(fen)(fen)鐘(zhong). 10. 終(zhong)止：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)(ye)50μl，終(zhong)止反應(ying)(ying)(ying)（此時(shi)藍(lan)色(se)(se)立轉黃色(se)(se)）。11. 測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)：以(yi)空(kong)(kong)白空(kong)(kong)調(diao)零，450nm波長依(yi)序測(ce)(ce)(ce)(ce)(ce)量(liang)(liang)各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)吸光(guang)度(du)(du)(du)(du)（OD值(zhi)(zhi)(zhi)）。 測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)應(ying)(ying)(ying)在(zai)(zai)(zai)(zai)加(jia)(jia)(jia)(jia)(jia)終(zhong)止液(ye)(ye)(ye)(ye)后(hou)(hou)(hou)(hou)15分(fen)(fen)(fen)鐘(zhong)以(yi)內(nei)進(jin)行(xing)。【注意(yi)事項】1． 試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)從冷(leng)藏環境(jing)中(zhong)取(qu)出(chu)(chu)應(ying)(ying)(ying)在(zai)(zai)(zai)(zai)室溫(wen)平衡15-30分(fen)(fen)(fen)鐘(zhong)后(hou)(hou)(hou)(hou)方可(ke)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)，酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)包被板(ban)(ban)(ban)(ban)開封(feng)(feng)后(hou)(hou)(hou)(hou)如(ru)(ru)未(wei)用(yong)(yong)(yong)(yong)完，板(ban)(ban)(ban)(ban)條應(ying)(ying)(ying)裝入(ru)(ru)密(mi)(mi)封(feng)(feng)袋中(zhong)保(bao)存(cun)。2． 濃(nong)(nong)(nong)(nong)洗(xi)滌液(ye)(ye)(ye)(ye)可(ke)能(neng)會有(you)(you)(you)(you)結晶(jing)析出(chu)(chu)，稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)時(shi)可(ke)在(zai)(zai)(zai)(zai)水(shui)(shui)浴(yu)中(zhong)加(jia)(jia)(jia)(jia)(jia)溫(wen)助溶，洗(xi)滌時(shi)不影響(xiang)結果。3． 各(ge)(ge)步(bu)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)均(jun)應(ying)(ying)(ying)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)器，并(bing)經(jing)常校對其(qi)準(zhun)(zhun)(zhun)確(que)性(xing)，以(yi)避(bi)(bi)免試(shi)(shi)(shi)驗(yan)誤(wu)差。一(yi)(yi)(yi)(yi)(yi)(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)間(jian)＊好控制在(zai)(zai)(zai)(zai)5分(fen)(fen)(fen)鐘(zhong)內(nei)，如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)數(shu)(shu)(shu)量(liang)(liang)多，推(tui)薦使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)排(pai)槍加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。4． 請(qing)每(mei)次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)的(de)(de)(de)(de)(de)同(tong)時(shi)做標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲線，＊好做復(fu)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)。如(ru)(ru)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)中(zhong)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)物(wu)(wu)(wu)(wu)質(zhi)(zhi)含量(liang)(liang)過高(gao)（樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)OD值(zhi)(zhi)(zhi)大(da)(da)于標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)第(di)(di)(di)(di)(di)(di)(di)(di)(di)(di)一(yi)(yi)(yi)(yi)(yi)(yi)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)），請(qing)先(xian)(xian)用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)一(yi)(yi)(yi)(yi)(yi)(yi)定(ding)(ding)倍(bei)(bei)(bei)數(shu)(shu)(shu)（n倍(bei)(bei)(bei)）后(hou)(hou)(hou)(hou)再(zai)(zai)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)，計算(suan)時(shi)請(qing)＊后(hou)(hou)(hou)(hou)乘(cheng)以(yi)總(zong)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)倍(bei)(bei)(bei)數(shu)(shu)(shu)（×n×5）。5． 封(feng)(feng)板(ban)(ban)(ban)(ban)膜(mo)(mo)只限一(yi)(yi)(yi)(yi)(yi)(yi)次(ci)(ci)性(xing)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)，以(yi)避(bi)(bi)免交叉污染(ran)。6． 底物(wu)(wu)(wu)(wu)請(qing)避(bi)(bi)光(guang)保(bao)存(cun)。7． 嚴格(ge)按照說明(ming)(ming)書的(de)(de)(de)(de)(de)操(cao)(cao)(cao)作(zuo)(zuo)進(jin)行(xing)，試(shi)(shi)(shi)驗(yan)結果判定(ding)(ding)必須以(yi)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)儀(yi)讀數(shu)(shu)(shu)為準(zhun)(zhun)(zhun).8． 所有(you)(you)(you)(you)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)，洗(xi)滌液(ye)(ye)(ye)(ye)和各(ge)(ge)種廢棄(qi)物(wu)(wu)(wu)(wu)都應(ying)(ying)(ying)按傳染(ran)物(wu)(wu)(wu)(wu)處理。9． 本(ben)(ben)(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)不同(tong)批號組分(fen)(fen)(fen)不得混(hun)(hun)(hun)用(yong)(yong)(yong)(yong)。10. 如(ru)(ru)與(yu)(yu)(yu)英文(wen)說明(ming)(ming)書有(you)(you)(you)(you)異，以(yi)英文(wen)說明(ming)(ming)書為準(zhun)(zhun)(zhun)。【計算(suan)】 以(yi)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)為橫(heng)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，OD值(zhi)(zhi)(zhi)為縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)，    在(zai)(zai)(zai)(zai)坐(zuo)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)紙上繪出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲線，根據(ju)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)OD      值(zhi)(zhi)(zhi)由(you)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲線查(cha)出(chu)(chu)相(xiang)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)；再(zai)(zai)乘(cheng)以(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)      倍(bei)(bei)(bei)數(shu)(shu)(shu)；或(huo)用(yong)(yong)(yong)(yong)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)與(yu)(yu)(yu)OD值(zhi)(zhi)(zhi)計算(suan)出(chu)(chu)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)      準(zhun)(zhun)(zhun)曲線的(de)(de)(de)(de)(de)直(zhi)線回歸方程式(shi)，將(jiang)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)OD值(zhi)(zhi)(zhi)      代入(ru)(ru)方程式(shi)，計算(suan)出(chu)(chu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)，再(zai)(zai)乘(cheng)以(yi)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)      倍(bei)(bei)(bei)數(shu)(shu)(shu)，即為樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)實際濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)。                   【試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)組成】試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)組成48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置保(bao)存(cun)說明(ming)(ming)書1份(fen)1份(fen)密(mi)(mi)封(feng)(feng)袋1個(ge)1個(ge)封(feng)(feng)板(ban)(ban)(ban)(ban)膜(mo)(mo)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條2片（96）2-8℃保(bao)存(cun)微(wei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)1×4812孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條2-8℃保(bao)存(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管2-8℃保(bao)存(cun)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)1.5ml×1瓶(ping)(ping)(ping)(ping)1.5ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)稀(xi)(xi)(xi)(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)顯(xian)(xian)(xian)色(se)(se)劑(ji)(ji)(ji)(ji)(ji)A液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)顯(xian)(xian)(xian)色(se)(se)劑(ji)(ji)(ji)(ji)(ji)B液(ye)(ye)(ye)(ye)3 ml×1瓶(ping)(ping)(ping)(ping)6 ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)終(zhong)止液(ye)(ye)(ye)(ye)3ml×1瓶(ping)(ping)(ping)(ping)6ml×1瓶(ping)(ping)(ping)(ping)2-8℃保(bao)存(cun)注意(yi)：使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)前(qian)請(qing)檢(jian)查(cha)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)中(zhong)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)(biao)(biao)(biao)簽和數(shu)(shu)(shu)量(liang)(liang)與(yu)(yu)(yu)表(biao)格(ge)是否一(yi)(yi)(yi)(yi)(yi)(yi)致。【精密(mi)(mi)度(du)(du)(du)(du)】精密(mi)(mi)度(du)(du)(du)(du)用(yong)(yong)(yong)(yong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)值(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)變(bian)異系數(shu)(shu)(shu)CV 表(biao)示。CV（%） = SD/mean×100批內(nei)差：取(qu)同(tong)批次(ci)(ci)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)對低、中(zhong)、高(gao)值(zhi)(zhi)(zhi)定(ding)(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)進(jin)行(xing)定(ding)(ding)量(liang)(liang)檢(jian)測(ce)(ce)(ce)(ce)(ce)，每(mei)份(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)連(lian)續測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)20 次(ci)(ci)，分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)計算(suan)不同(tong)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)平均(jun)值(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)。批內(nei)差： CV<9%批間(jian)差：選取(qu)3 個(ge)不同(tong)批次(ci)(ci)的(de)(de)(de)(de)(de)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)對低、中(zhong)、高(gao)值(zhi)(zhi)(zhi)定(ding)(ding)值(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)進(jin)行(xing)定(ding)(ding)量(liang)(liang)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)，每(mei)個(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)同(tong)一(yi)(yi)(yi)(yi)(yi)(yi)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)重(zhong)(zhong)復(fu)測(ce)(ce)(ce)(ce)(ce)定(ding)(ding)10 次(ci)(ci)，分(fen)(fen)(fen)別(bie)(bie)(bie)(bie)計算(suan)不同(tong)濃(nong)(nong)(nong)(nong)度(du)(du)(du)(du)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)的(de)(de)(de)(de)(de)平均(jun)值(zhi)(zhi)(zhi)及(ji)SD 值(zhi)(zhi)(zhi)。批間(jian)差： CV<11% 【特異性(xing)】本(ben)(ben)(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)識(shi)別(bie)(bie)(bie)(bie)天然(ran)(ran)和重(zhong)(zhong)組微(wei)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)硝酸鹽還原酶(mei)(mei)（Nar），經(jing)檢(jian)測(ce)(ce)(ce)(ce)(ce)與(yu)(yu)(yu)其(qi)它相(xiang)似物(wu)(wu)(wu)(wu)質(zhi)(zhi)無明(ming)(ming)顯(xian)(xian)(xian)交叉反應(ying)(ying)(ying)。由(you)于受到(dao)技術及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)來(lai)源的(de)(de)(de)(de)(de)限制，不可(ke)能(neng)完成所有(you)(you)(you)(you)相(xiang)關或(huo)相(xiang)似物(wu)(wu)(wu)(wu)質(zhi)(zhi)交叉反應(ying)(ying)(ying)檢(jian)測(ce)(ce)(ce)(ce)(ce)，因(yin)此本(ben)(ben)(ben)試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)有(you)(you)(you)(you)可(ke)能(neng)與(yu)(yu)(yu)未(wei)經(jing)檢(jian)測(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)其(qi)它物(wu)(wu)(wu)(wu)質(zhi)(zhi)有(you)(you)(you)(you)交叉反應(ying)(ying)(ying)。 【穩定(ding)(ding)性(xing)】試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)在(zai)(zai)(zai)(zai)有(you)(you)(you)(you)效期內(nei)務必按推(tui)薦溫(wen)度(du)(du)(du)(du)保(bao)存(cun)，活性(xing)降低率小(xiao)于5%。為減小(xiao)外部(bu)因(yin)素對試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)盒(he)(he)破壞前(qian)后(hou)(hou)(hou)(hou)檢(jian)測(ce)(ce)(ce)(ce)(ce)值(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)影響(xiang)，實驗(yan)室的(de)(de)(de)(de)(de)環境(jing)條件需盡(jin)量(liang)(liang)保(bao)持一(yi)(yi)(yi)(yi)(yi)(yi)致，尤其(qi)是實驗(yan)室內(nei)溫(wen)度(du)(du)(du)(du)、濕(shi)度(du)(du)(du)(du)及(ji)溫(wen)育(yu)條件。其(qi)次(ci)(ci)由(you)同(tong)一(yi)(yi)(yi)(yi)(yi)(yi)實驗(yan)員來(lai)進(jin)行(xing)操(cao)(cao)(cao)作(zuo)(zuo)可(ke)減少人為誤(wu)差。

大鼠(shu)(shu)(shu)(shu)睪酮(tong)(tong)(tong)(T)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he) 大鼠(shu)(shu)(shu)(shu)睪酮(tong)(tong)(tong)(T)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)檢(jian)(jian)(jian)測(ce)(ce)原(yuan)理試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)采(cai)用(yong)雙抗體(ti)(ti)一(yi)步夾心(xin)法(fa)酶聯免(mian)疫(yi)吸(xi)附試(shi)(shi)(shi)(shi)驗(yan)（ELISA）。往預先包被(bei)活性(xing)氧(yang)簇（ROS）抗體(ti)(ti)的(de)(de)(de)(de)包被(bei)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，依次(ci)(ci)加(jia)入(ru)標(biao)(biao)(biao)(biao)本(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)抗體(ti)(ti)，經(jing)過(guo)溫(wen)(wen)(wen)育(yu)(yu)并徹底洗(xi)(xi)滌(di)。用(yong)底物(wu)TMB顯色(se)，TMB在(zai)(zai)過(guo)氧(yang)化(hua)物(wu)酶的(de)(de)(de)(de)催(cui)化(hua)下(xia)(xia)轉(zhuan)化(hua)成(cheng)藍色(se)，并在(zai)(zai)酸(suan)的(de)(de)(de)(de)作(zuo)(zuo)用(yong)下(xia)(xia)轉(zhuan)化(hua)成(cheng)＊終的(de)(de)(de)(de)黃色(se)。顏色(se)的(de)(de)(de)(de)深淺和(he)樣(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)活性(xing)氧(yang)簇（ROS）呈正相關。用(yong)酶標(biao)(biao)(biao)(biao)儀(yi)在(zai)(zai)450nm 波(bo)長下(xia)(xia)測(ce)(ce)定吸(xi)光度(du)（OD 值），計算(suan)樣(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)活性(xing)。大鼠(shu)(shu)(shu)(shu)睪酮(tong)(tong)(tong)(T)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)樣(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)收集(ji)、處(chu)理及保(bao)存方法(fa)1.  血(xue)清(qing)：使用(yong)不含熱原(yuan)和(he)內毒(du)素(su)的(de)(de)(de)(de)試(shi)(shi)(shi)(shi)管(guan)，操(cao)(cao)作(zuo)(zuo)過(guo)程(cheng)中(zhong)(zhong)避(bi)(bi)(bi)免(mian)任何(he)細胞刺激，收集(ji)血(xue)液(ye)(ye)(ye)(ye)后(hou)，3000轉(zhuan)離(li)心(xin)10分(fen)(fen)(fen)(fen)(fen)(fen)鐘將(jiang)血(xue)清(qing)和(he)紅細胞迅速小心(xin)地(di)分(fen)(fen)(fen)(fen)(fen)(fen)離(li)。2.  血(xue)漿：EDTA、檸檬酸(suan)鹽(yan)或(huo)肝素(su)抗凝。3000轉(zhuan)離(li)心(xin)30分(fen)(fen)(fen)(fen)(fen)(fen)鐘取上(shang)清(qing)。3.  細胞上(shang)清(qing)液(ye)(ye)(ye)(ye)：3000轉(zhuan)離(li)心(xin)10分(fen)(fen)(fen)(fen)(fen)(fen)鐘去除(chu)顆粒和(he)聚合物(wu)。4.  組織(zhi)勻漿：將(jiang)組織(zhi)加(jia)入(ru)適量生理鹽(yan)水(shui)搗(dao)碎。3000轉(zhuan)離(li)心(xin)10分(fen)(fen)(fen)(fen)(fen)(fen)鐘取上(shang)清(qing)。5.  保(bao)存：如果(guo)樣(yang)(yang)本(ben)(ben)(ben)收集(ji)后(hou)不及時(shi)(shi)檢(jian)(jian)(jian)測(ce)(ce)，請按一(yi)次(ci)(ci)用(yong)量分(fen)(fen)(fen)(fen)(fen)(fen)裝，凍存于(yu)(yu)-20℃，避(bi)(bi)(bi)免(mian)反(fan)復凍融，在(zai)(zai)室溫(wen)(wen)(wen)下(xia)(xia)解凍并確保(bao)樣(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)均(jun)勻地(di)充分(fen)(fen)(fen)(fen)(fen)(fen)解凍。大鼠(shu)(shu)(shu)(shu)睪酮(tong)(tong)(tong)(T)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)自(zi)備物(wu)品(pin)(pin)(pin)(pin)(pin)(pin)1.酶標(biao)(biao)(biao)(biao)儀(yi)（450nm）2.高精度(du)加(jia)樣(yang)(yang)器及槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3.37℃恒溫(wen)(wen)(wen)箱操(cao)(cao)作(zuo)(zuo)注(zhu)意(yi)事項1.試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)保(bao)存在(zai)(zai)2-8℃，使用(yong)前(qian)室溫(wen)(wen)(wen)平(ping)衡20分(fen)(fen)(fen)(fen)(fen)(fen)鐘。從(cong)冰(bing)箱取出的(de)(de)(de)(de)濃(nong)(nong)(nong)縮洗(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)會有結晶，這(zhe)屬(shu)于(yu)(yu)正常現象，水(shui)浴加(jia)熱使結晶完全溶解后(hou)再使用(yong)。2.實(shi)(shi)(shi)驗(yan)中(zhong)(zhong)不用(yong)的(de)(de)(de)(de)板(ban)(ban)(ban)(ban)條(tiao)應立即放回(hui)自(zi)封袋(dai)(dai)中(zhong)(zhong)，密(mi)封（低(di)溫(wen)(wen)(wen)干(gan)燥）保(bao)存。3.濃(nong)(nong)(nong)度(du)為(wei)0的(de)(de)(de)(de)S0號標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)即可(ke)視為(wei)陰性(xing)對照或(huo)者(zhe)空白；按照說(shuo)(shuo)明(ming)(ming)書(shu)(shu)操(cao)(cao)作(zuo)(zuo)時(shi)(shi)樣(yang)(yang)本(ben)(ben)(ben)已經(jing)稀(xi)(xi)(xi)釋(shi)(shi)5倍，＊終結果(guo)乘以5才是樣(yang)(yang)本(ben)(ben)(ben)實(shi)(shi)(shi)際(ji)濃(nong)(nong)(nong)度(du)。4.嚴(yan)格按照說(shuo)(shuo)明(ming)(ming)書(shu)(shu)中(zhong)(zhong)標(biao)(biao)(biao)(biao)明(ming)(ming)的(de)(de)(de)(de)時(shi)(shi)間、加(jia)液(ye)(ye)(ye)(ye)量及順序進(jin)行溫(wen)(wen)(wen)育(yu)(yu)操(cao)(cao)作(zuo)(zuo)。5.所有液(ye)(ye)(ye)(ye)體(ti)(ti)組分(fen)(fen)(fen)(fen)(fen)(fen)使用(yong)前(qian)充分(fen)(fen)(fen)(fen)(fen)(fen)搖勻。試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)組成(cheng)名稱96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配置(zhi)備注(zhu)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶標(biao)(biao)(biao)(biao)板(ban)(ban)(ban)(ban)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)無(wu)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管(guan)0.3mL*6管(guan)無(wu)樣(yang)(yang)本(ben)(ben)(ben)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)6mL3mL無(wu)檢(jian)(jian)(jian)測(ce)(ce)抗體(ti)(ti)-HRP10mL5mL無(wu)20×洗(xi)(xi)滌(di)緩(huan)沖液(ye)(ye)(ye)(ye)25mL15mL按說(shuo)(shuo)明(ming)(ming)書(shu)(shu)進(jin)行稀(xi)(xi)(xi)釋(shi)(shi)底物(wu)A6mL3mL無(wu)底物(wu)B6mL3mL無(wu)終止(zhi)(zhi)液(ye)(ye)(ye)(ye)6mL3mL無(wu)封板(ban)(ban)(ban)(ban)膜2張2張無(wu)說(shuo)(shuo)明(ming)(ming)書(shu)(shu)1份(fen)(fen)1份(fen)(fen)無(wu)自(zi)封袋(dai)(dai)1個1個無(wu)注(zhu)：標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)（S0-S5）濃(nong)(nong)(nong)度(du)依次(ci)(ci)為(wei)：0、30、60、120、240、480 U/ml試(shi)(shi)(shi)(shi)劑(ji)(ji)的(de)(de)(de)(de)準(zhun)(zhun)備 20×洗(xi)(xi)滌(di)緩(huan)沖液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)：蒸(zheng)餾水(shui)按1：20稀(xi)(xi)(xi)釋(shi)(shi)，即1份(fen)(fen)的(de)(de)(de)(de)20×洗(xi)(xi)滌(di)緩(huan)沖液(ye)(ye)(ye)(ye)加(jia)19份(fen)(fen)的(de)(de)(de)(de)蒸(zheng)餾水(shui)。洗(xi)(xi)板(ban)(ban)(ban)(ban)方法(fa)1.手(shou)工洗(xi)(xi)板(ban)(ban)(ban)(ban)：甩(shuai)(shuai)盡孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內液(ye)(ye)(ye)(ye)體(ti)(ti)，每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)滿洗(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)，靜置(zhi)1min后(hou)甩(shuai)(shuai)盡孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內液(ye)(ye)(ye)(ye)體(ti)(ti)，在(zai)(zai)吸(xi)水(shui)紙上(shang)拍干(gan)，如此洗(xi)(xi)板(ban)(ban)(ban)(ban)5次(ci)(ci)。2.自(zi)動洗(xi)(xi)板(ban)(ban)(ban)(ban)機(ji)：每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)注(zhu)入(ru)洗(xi)(xi)液(ye)(ye)(ye)(ye)350μL，浸泡1min，洗(xi)(xi)板(ban)(ban)(ban)(ban)5次(ci)(ci)。操(cao)(cao)作(zuo)(zuo)步驟1.從(cong)室溫(wen)(wen)(wen)平(ping)衡20min后(hou)的(de)(de)(de)(de)鋁(lv)箔袋(dai)(dai)中(zhong)(zhong)取出所需板(ban)(ban)(ban)(ban)條(tiao)，剩余板(ban)(ban)(ban)(ban)條(tiao)用(yong)自(zi)封袋(dai)(dai)密(mi)封放回(hui)4℃。2.設置(zhi)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)樣(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)各(ge)加(jia)不同濃(nong)(nong)(nong)度(du)的(de)(de)(de)(de)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)50μL；3.樣(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先加(jia)待測(ce)(ce)樣(yang)(yang)本(ben)(ben)(ben)10μL，再加(jia)樣(yang)(yang)本(ben)(ben)(ben)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)(ye)(ye)40μL；空白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不加(jia)。4.除(chu)空白孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)外，標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)樣(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)入(ru)辣根過(guo)氧(yang)化(hua)物(wu)酶（HRP）標(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)抗體(ti)(ti)100μL，用(yong)封板(ban)(ban)(ban)(ban)膜封住反(fan)應孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，37℃水(shui)浴鍋或(huo)恒溫(wen)(wen)(wen)箱溫(wen)(wen)(wen)育(yu)(yu)60min。5.棄去液(ye)(ye)(ye)(ye)體(ti)(ti)，吸(xi)水(shui)紙上(shang)拍干(gan)，每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)滿洗(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)，靜置(zhi)1min，甩(shuai)(shuai)去洗(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)，吸(xi)水(shui)紙上(shang)拍干(gan)，如此重復洗(xi)(xi)板(ban)(ban)(ban)(ban)5次(ci)(ci)（也可(ke)用(yong)洗(xi)(xi)板(ban)(ban)(ban)(ban)機(ji)洗(xi)(xi)板(ban)(ban)(ban)(ban)）。6.每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)入(ru)底物(wu)A、B各(ge)50μL，37℃避(bi)(bi)(bi)光孵育(yu)(yu)15min。7.每孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)入(ru)終止(zhi)(zhi)液(ye)(ye)(ye)(ye)50μL，15min內，在(zai)(zai)450nm波(bo)長處(chu)測(ce)(ce)定各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)OD值。結果(guo)判斷 繪(hui)制標(biao)(biao)(biao)(biao)準(zhun)(zhun)曲線(xian)：在(zai)(zai)Excel工作(zuo)(zuo)表中(zhong)(zhong)，以標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)作(zuo)(zuo)橫(heng)坐標(biao)(biao)(biao)(biao)，對應OD值作(zuo)(zuo)縱坐標(biao)(biao)(biao)(biao)，繪(hui)制出標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)線(xian)性(xing)回(hui)歸(gui)曲線(xian)，按曲線(xian)方程(cheng)計算(suan)各(ge)樣(yang)(yang)本(ben)(ben)(ben)濃(nong)(nong)(nong)度(du)值。大鼠(shu)(shu)(shu)(shu)睪酮(tong)(tong)(tong)(T)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)性(xing)能1.準(zhun)(zhun)確性(xing)：標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)線(xian)性(xing)回(hui)歸(gui)與預期(qi)濃(nong)(nong)(nong)度(du)相關系數(shu)R值，大于(yu)(yu)等于(yu)(yu)0.9900。2.靈敏度(du)：＊低(di)檢(jian)(jian)(jian)測(ce)(ce)濃(nong)(nong)(nong)度(du)小于(yu)(yu)1.0 U/ml。3.特異(yi)性(xing)：不與其它(ta)可(ke)溶性(xing)結構類(lei)似(si)物(wu)交叉反(fan)應。4.重復性(xing)：板(ban)(ban)(ban)(ban)內、板(ban)(ban)(ban)(ban)間變異(yi)系數(shu)均(jun)小于(yu)(yu)15%。5.貯藏：2-8℃，避(bi)(bi)(bi)光防(fang)潮保(bao)存。6.有效期(qi)：6個月免(mian)責聲明(ming)(ming)1.試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)僅供研究使用(yong)，不得用(yong)于(yu)(yu)臨床(chuang)實(shi)(shi)(shi)驗(yan)或(huo)人體(ti)(ti)實(shi)(shi)(shi)驗(yan)，否(fou)則所產生的(de)(de)(de)(de)一(yi)切后(hou)果(guo)，由(you)實(shi)(shi)(shi)驗(yan)者(zhe)承擔，本(ben)(ben)(ben)公司概不負責。2.嚴(yan)格按照說(shuo)(shuo)明(ming)(ming)書(shu)(shu)操(cao)(cao)作(zuo)(zuo)，實(shi)(shi)(shi)驗(yan)者(zhe)違反(fan)說(shuo)(shuo)明(ming)(ming)書(shu)(shu)操(cao)(cao)作(zuo)(zuo)，后(hou)果(guo)由(you)實(shi)(shi)(shi)驗(yan)者(zhe)承擔。 大鼠(shu)(shu)(shu)(shu)睪酮(tong)(tong)(tong)(T)ELISA試(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he) 

大鼠(shu)(shu)雙氫睪(gao)(gao)酮(tong)(tong)(tong)(DHT)ELISA試(shi)(shi)(shi)劑(ji)盒(he) 大鼠(shu)(shu)雙氫睪(gao)(gao)酮(tong)(tong)(tong)(DHT)ELISA試(shi)(shi)(shi)劑(ji)盒(he)檢(jian)測(ce)原(yuan)理(li)(li)試(shi)(shi)(shi)劑(ji)盒(he)采用(yong)雙抗(kang)(kang)體(ti)(ti)(ti)(ti)一步(bu)夾心(xin)(xin)法(fa)(fa)酶(mei)(mei)聯(lian)免(mian)疫吸(xi)附試(shi)(shi)(shi)驗(yan)(yan)（ELISA）。往預先包(bao)被活性(xing)(xing)氧簇（ROS）抗(kang)(kang)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)包(bao)被微(wei)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)，依(yi)次加(jia)(jia)(jia)(jia)入標(biao)(biao)(biao)(biao)(biao)本(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)(de)檢(jian)測(ce)抗(kang)(kang)體(ti)(ti)(ti)(ti)，經過(guo)溫(wen)育(yu)并(bing)(bing)徹(che)底(di)洗(xi)(xi)(xi)滌(di)(di)。用(yong)底(di)物(wu)(wu)TMB顯色(se)(se)，TMB在(zai)(zai)過(guo)氧化(hua)物(wu)(wu)酶(mei)(mei)的(de)(de)(de)(de)(de)催化(hua)下(xia)轉(zhuan)化(hua)成藍色(se)(se)，并(bing)(bing)在(zai)(zai)酸的(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)用(yong)下(xia)轉(zhuan)化(hua)成＊終的(de)(de)(de)(de)(de)黃色(se)(se)。顏色(se)(se)的(de)(de)(de)(de)(de)深淺和(he)(he)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)(de)活性(xing)(xing)氧簇（ROS）呈正(zheng)相關(guan)。用(yong)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)450nm 波(bo)長(chang)下(xia)測(ce)定吸(xi)光度(du)(du)（OD 值(zhi)），計算樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)活性(xing)(xing)。大鼠(shu)(shu)雙氫睪(gao)(gao)酮(tong)(tong)(tong)(DHT)ELISA試(shi)(shi)(shi)劑(ji)盒(he)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)收集、處理(li)(li)及(ji)保(bao)(bao)存(cun)方(fang)(fang)法(fa)(fa)1.  血(xue)(xue)清：使(shi)用(yong)不(bu)(bu)含熱(re)(re)原(yuan)和(he)(he)內(nei)(nei)毒素的(de)(de)(de)(de)(de)試(shi)(shi)(shi)管，操(cao)(cao)作(zuo)(zuo)(zuo)過(guo)程中(zhong)(zhong)避免(mian)任何細(xi)胞刺激，收集血(xue)(xue)液(ye)(ye)(ye)后(hou)(hou)，3000轉(zhuan)離心(xin)(xin)10分(fen)(fen)(fen)(fen)鐘(zhong)將血(xue)(xue)清和(he)(he)紅細(xi)胞迅速小(xiao)心(xin)(xin)地分(fen)(fen)(fen)(fen)離。2.  血(xue)(xue)漿：EDTA、檸(ning)檬酸鹽或(huo)肝素抗(kang)(kang)凝。3000轉(zhuan)離心(xin)(xin)30分(fen)(fen)(fen)(fen)鐘(zhong)取(qu)上(shang)清。3.  細(xi)胞上(shang)清液(ye)(ye)(ye)：3000轉(zhuan)離心(xin)(xin)10分(fen)(fen)(fen)(fen)鐘(zhong)去(qu)除顆粒和(he)(he)聚(ju)合(he)物(wu)(wu)。4.  組(zu)織(zhi)勻(yun)漿：將組(zu)織(zhi)加(jia)(jia)(jia)(jia)入適量生(sheng)理(li)(li)鹽水(shui)(shui)搗碎。3000轉(zhuan)離心(xin)(xin)10分(fen)(fen)(fen)(fen)鐘(zhong)取(qu)上(shang)清。5.  保(bao)(bao)存(cun)：如(ru)果樣(yang)(yang)(yang)本(ben)(ben)(ben)收集后(hou)(hou)不(bu)(bu)及(ji)時檢(jian)測(ce)，請按一次用(yong)量分(fen)(fen)(fen)(fen)裝，凍存(cun)于(yu)(yu)-20℃，避免(mian)反(fan)復(fu)(fu)凍融，在(zai)(zai)室(shi)溫(wen)下(xia)解凍并(bing)(bing)確保(bao)(bao)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)均勻(yun)地充分(fen)(fen)(fen)(fen)解凍。大鼠(shu)(shu)雙氫睪(gao)(gao)酮(tong)(tong)(tong)(DHT)ELISA試(shi)(shi)(shi)劑(ji)盒(he)自(zi)備物(wu)(wu)品(pin)(pin)(pin)(pin)1.酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)儀（450nm）2.高精度(du)(du)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)器及(ji)槍(qiang)頭(tou)：0.5-10uL、2-20uL、20-200uL、200-1000uL3.37℃恒(heng)溫(wen)箱(xiang)操(cao)(cao)作(zuo)(zuo)(zuo)注意事(shi)項1.試(shi)(shi)(shi)劑(ji)盒(he)保(bao)(bao)存(cun)在(zai)(zai)2-8℃，使(shi)用(yong)前室(shi)溫(wen)平衡(heng)20分(fen)(fen)(fen)(fen)鐘(zhong)。從(cong)冰箱(xiang)取(qu)出的(de)(de)(de)(de)(de)濃(nong)(nong)(nong)縮洗(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)會有(you)結(jie)(jie)晶(jing)，這屬于(yu)(yu)正(zheng)常現(xian)象，水(shui)(shui)浴(yu)加(jia)(jia)(jia)(jia)熱(re)(re)使(shi)結(jie)(jie)晶(jing)完全(quan)溶解后(hou)(hou)再(zai)使(shi)用(yong)。2.實驗(yan)(yan)中(zhong)(zhong)不(bu)(bu)用(yong)的(de)(de)(de)(de)(de)板(ban)(ban)條(tiao)(tiao)應立即放回(hui)(hui)自(zi)封(feng)(feng)(feng)袋中(zhong)(zhong)，密(mi)封(feng)(feng)(feng)（低溫(wen)干(gan)(gan)燥）保(bao)(bao)存(cun)。3.濃(nong)(nong)(nong)度(du)(du)為(wei)0的(de)(de)(de)(de)(de)S0號標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)即可(ke)視(shi)為(wei)陰(yin)性(xing)(xing)對(dui)照(zhao)(zhao)或(huo)者(zhe)空(kong)(kong)白；按照(zhao)(zhao)說(shuo)(shuo)明(ming)書(shu)(shu)(shu)(shu)操(cao)(cao)作(zuo)(zuo)(zuo)時樣(yang)(yang)(yang)本(ben)(ben)(ben)已經稀(xi)釋(shi)(shi)5倍(bei)，＊終結(jie)(jie)果乘以5才是樣(yang)(yang)(yang)本(ben)(ben)(ben)實際濃(nong)(nong)(nong)度(du)(du)。4.嚴格按照(zhao)(zhao)說(shuo)(shuo)明(ming)書(shu)(shu)(shu)(shu)中(zhong)(zhong)標(biao)(biao)(biao)(biao)(biao)明(ming)的(de)(de)(de)(de)(de)時間(jian)、加(jia)(jia)(jia)(jia)液(ye)(ye)(ye)量及(ji)順序(xu)進行溫(wen)育(yu)操(cao)(cao)作(zuo)(zuo)(zuo)。5.所(suo)有(you)液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)組(zu)分(fen)(fen)(fen)(fen)使(shi)用(yong)前充分(fen)(fen)(fen)(fen)搖(yao)勻(yun)。試(shi)(shi)(shi)劑(ji)盒(he)組(zu)成名稱96孔(kong)(kong)(kong)(kong)配置(zhi)(zhi)48孔(kong)(kong)(kong)(kong)配置(zhi)(zhi)備注微(wei)孔(kong)(kong)(kong)(kong)酶(mei)(mei)標(biao)(biao)(biao)(biao)(biao)板(ban)(ban)12孔(kong)(kong)(kong)(kong)×8條(tiao)(tiao)12孔(kong)(kong)(kong)(kong)×4條(tiao)(tiao)無(wu)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管無(wu)樣(yang)(yang)(yang)本(ben)(ben)(ben)稀(xi)釋(shi)(shi)液(ye)(ye)(ye)6mL3mL無(wu)檢(jian)測(ce)抗(kang)(kang)體(ti)(ti)(ti)(ti)-HRP10mL5mL無(wu)20×洗(xi)(xi)(xi)滌(di)(di)緩沖液(ye)(ye)(ye)25mL15mL按說(shuo)(shuo)明(ming)書(shu)(shu)(shu)(shu)進行稀(xi)釋(shi)(shi)底(di)物(wu)(wu)A6mL3mL無(wu)底(di)物(wu)(wu)B6mL3mL無(wu)終止液(ye)(ye)(ye)6mL3mL無(wu)封(feng)(feng)(feng)板(ban)(ban)膜2張2張無(wu)說(shuo)(shuo)明(ming)書(shu)(shu)(shu)(shu)1份1份無(wu)自(zi)封(feng)(feng)(feng)袋1個(ge)1個(ge)無(wu)注：標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)（S0-S5）濃(nong)(nong)(nong)度(du)(du)依(yi)次為(wei)：0、30、60、120、240、480 U/ml試(shi)(shi)(shi)劑(ji)的(de)(de)(de)(de)(de)準(zhun)(zhun)備 20×洗(xi)(xi)(xi)滌(di)(di)緩沖液(ye)(ye)(ye)的(de)(de)(de)(de)(de)稀(xi)釋(shi)(shi)：蒸(zheng)餾(liu)水(shui)(shui)按1：20稀(xi)釋(shi)(shi)，即1份的(de)(de)(de)(de)(de)20×洗(xi)(xi)(xi)滌(di)(di)緩沖液(ye)(ye)(ye)加(jia)(jia)(jia)(jia)19份的(de)(de)(de)(de)(de)蒸(zheng)餾(liu)水(shui)(shui)。洗(xi)(xi)(xi)板(ban)(ban)方(fang)(fang)法(fa)(fa)1.手(shou)工(gong)洗(xi)(xi)(xi)板(ban)(ban)：甩盡孔(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，每(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿(man)(man)洗(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)，靜置(zhi)(zhi)1min后(hou)(hou)甩盡孔(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，在(zai)(zai)吸(xi)水(shui)(shui)紙上(shang)拍干(gan)(gan)，如(ru)此洗(xi)(xi)(xi)板(ban)(ban)5次。2.自(zi)動洗(xi)(xi)(xi)板(ban)(ban)機(ji)(ji)：每(mei)孔(kong)(kong)(kong)(kong)注入洗(xi)(xi)(xi)液(ye)(ye)(ye)350μL，浸泡1min，洗(xi)(xi)(xi)板(ban)(ban)5次。操(cao)(cao)作(zuo)(zuo)(zuo)步(bu)驟(zou)1.從(cong)室(shi)溫(wen)平衡(heng)20min后(hou)(hou)的(de)(de)(de)(de)(de)鋁箔袋中(zhong)(zhong)取(qu)出所(suo)需板(ban)(ban)條(tiao)(tiao)，剩余板(ban)(ban)條(tiao)(tiao)用(yong)自(zi)封(feng)(feng)(feng)袋密(mi)封(feng)(feng)(feng)放回(hui)(hui)4℃。2.設置(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)和(he)(he)樣(yang)(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)，標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)各加(jia)(jia)(jia)(jia)不(bu)(bu)同濃(nong)(nong)(nong)度(du)(du)的(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)50μL；3.樣(yang)(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)待測(ce)樣(yang)(yang)(yang)本(ben)(ben)(ben)10μL，再(zai)加(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)本(ben)(ben)(ben)稀(xi)釋(shi)(shi)液(ye)(ye)(ye)40μL；空(kong)(kong)白孔(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)。4.除空(kong)(kong)白孔(kong)(kong)(kong)(kong)外，標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)和(he)(he)樣(yang)(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)中(zhong)(zhong)每(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入辣根過(guo)氧化(hua)物(wu)(wu)酶(mei)(mei)（HRP）標(biao)(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)(de)檢(jian)測(ce)抗(kang)(kang)體(ti)(ti)(ti)(ti)100μL，用(yong)封(feng)(feng)(feng)板(ban)(ban)膜封(feng)(feng)(feng)住(zhu)反(fan)應孔(kong)(kong)(kong)(kong)，37℃水(shui)(shui)浴(yu)鍋或(huo)恒(heng)溫(wen)箱(xiang)溫(wen)育(yu)60min。5.棄去(qu)液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)，吸(xi)水(shui)(shui)紙上(shang)拍干(gan)(gan)，每(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿(man)(man)洗(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)，靜置(zhi)(zhi)1min，甩去(qu)洗(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)，吸(xi)水(shui)(shui)紙上(shang)拍干(gan)(gan)，如(ru)此重復(fu)(fu)洗(xi)(xi)(xi)板(ban)(ban)5次（也可(ke)用(yong)洗(xi)(xi)(xi)板(ban)(ban)機(ji)(ji)洗(xi)(xi)(xi)板(ban)(ban)）。6.每(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入底(di)物(wu)(wu)A、B各50μL，37℃避光孵育(yu)15min。7.每(mei)孔(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入終止液(ye)(ye)(ye)50μL，15min內(nei)(nei)，在(zai)(zai)450nm波(bo)長(chang)處測(ce)定各孔(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)OD值(zhi)。結(jie)(jie)果判斷 繪制(zhi)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)線(xian)：在(zai)(zai)Excel工(gong)作(zuo)(zuo)(zuo)表中(zhong)(zhong)，以標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)作(zuo)(zuo)(zuo)橫坐標(biao)(biao)(biao)(biao)(biao)，對(dui)應OD值(zhi)作(zuo)(zuo)(zuo)縱坐標(biao)(biao)(biao)(biao)(biao)，繪制(zhi)出標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)線(xian)性(xing)(xing)回(hui)(hui)歸(gui)曲(qu)線(xian)，按曲(qu)線(xian)方(fang)(fang)程計算各樣(yang)(yang)(yang)本(ben)(ben)(ben)濃(nong)(nong)(nong)度(du)(du)值(zhi)。大鼠(shu)(shu)雙氫睪(gao)(gao)酮(tong)(tong)(tong)(DHT)ELISA試(shi)(shi)(shi)劑(ji)盒(he)試(shi)(shi)(shi)劑(ji)盒(he)性(xing)(xing)能1.準(zhun)(zhun)確性(xing)(xing)：標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)線(xian)性(xing)(xing)回(hui)(hui)歸(gui)與預期(qi)濃(nong)(nong)(nong)度(du)(du)相關(guan)系數R值(zhi)，大于(yu)(yu)等(deng)于(yu)(yu)0.9900。2.靈敏度(du)(du)：＊低檢(jian)測(ce)濃(nong)(nong)(nong)度(du)(du)小(xiao)于(yu)(yu)1.0 U/ml。3.特異性(xing)(xing)：不(bu)(bu)與其它可(ke)溶性(xing)(xing)結(jie)(jie)構(gou)類似(si)物(wu)(wu)交叉(cha)反(fan)應。4.重復(fu)(fu)性(xing)(xing)：板(ban)(ban)內(nei)(nei)、板(ban)(ban)間(jian)變異系數均小(xiao)于(yu)(yu)15%。5.貯藏：2-8℃，避光防潮(chao)保(bao)(bao)存(cun)。6.有(you)效期(qi)：6個(ge)月免(mian)責(ze)聲明(ming)1.試(shi)(shi)(shi)劑(ji)盒(he)僅供研(yan)究(jiu)使(shi)用(yong)，不(bu)(bu)得(de)用(yong)于(yu)(yu)臨床實驗(yan)(yan)或(huo)人(ren)體(ti)(ti)(ti)(ti)實驗(yan)(yan)，否(fou)則所(suo)產生(sheng)的(de)(de)(de)(de)(de)一切后(hou)(hou)果，由(you)實驗(yan)(yan)者(zhe)承擔(dan)，本(ben)(ben)(ben)公司概不(bu)(bu)負責(ze)。2.嚴格按照(zhao)(zhao)說(shuo)(shuo)明(ming)書(shu)(shu)(shu)(shu)操(cao)(cao)作(zuo)(zuo)(zuo)，實驗(yan)(yan)者(zhe)違反(fan)說(shuo)(shuo)明(ming)書(shu)(shu)(shu)(shu)操(cao)(cao)作(zuo)(zuo)(zuo)，后(hou)(hou)果由(you)實驗(yan)(yan)者(zhe)承擔(dan)。 大鼠(shu)(shu)雙氫睪(gao)(gao)酮(tong)(tong)(tong)(DHT)ELISA試(shi)(shi)(shi)劑(ji)盒(he) 

大(da)(da)鼠(shu)(shu)雄(xiong)酮(tong)(tong)(tong)(ASD)ELISA試(shi)(shi)劑(ji)(ji)盒(he)(he) 大(da)(da)鼠(shu)(shu)雄(xiong)酮(tong)(tong)(tong)(ASD)ELISA試(shi)(shi)劑(ji)(ji)盒(he)(he)檢測(ce)原理試(shi)(shi)劑(ji)(ji)盒(he)(he)采(cai)用(yong)(yong)(yong)(yong)雙抗體(ti)一步(bu)夾心(xin)(xin)法(fa)酶(mei)(mei)聯(lian)免(mian)疫(yi)吸(xi)附試(shi)(shi)驗（ELISA）。往(wang)預(yu)(yu)先(xian)包被(bei)活性(xing)(xing)(xing)(xing)氧(yang)(yang)(yang)簇（ROS）抗體(ti)的(de)(de)(de)(de)(de)包被(bei)微孔(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，依次加(jia)(jia)入(ru)標(biao)(biao)本(ben)(ben)(ben)、標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)記(ji)的(de)(de)(de)(de)(de)檢測(ce)抗體(ti)，經過溫(wen)(wen)(wen)(wen)育并徹底洗(xi)(xi)(xi)(xi)滌(di)(di)。用(yong)(yong)(yong)(yong)底物(wu)TMB顯色(se)，TMB在(zai)過氧(yang)(yang)(yang)化(hua)物(wu)酶(mei)(mei)的(de)(de)(de)(de)(de)催化(hua)下轉化(hua)成藍(lan)色(se)，并在(zai)酸的(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)下轉化(hua)成＊終的(de)(de)(de)(de)(de)黃色(se)。顏(yan)色(se)的(de)(de)(de)(de)(de)深淺和(he)(he)(he)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)活性(xing)(xing)(xing)(xing)氧(yang)(yang)(yang)簇（ROS）呈(cheng)正相(xiang)(xiang)關。用(yong)(yong)(yong)(yong)酶(mei)(mei)標(biao)(biao)儀在(zai)450nm 波長下測(ce)定吸(xi)光度(du)(du)(du)(du)（OD 值(zhi)(zhi)），計算樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)活性(xing)(xing)(xing)(xing)。大(da)(da)鼠(shu)(shu)雄(xiong)酮(tong)(tong)(tong)(ASD)ELISA試(shi)(shi)劑(ji)(ji)盒(he)(he)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)收集、處理及保(bao)(bao)存(cun)方法(fa)1.  血清(qing)(qing)：使用(yong)(yong)(yong)(yong)不(bu)(bu)含熱原和(he)(he)(he)內(nei)(nei)毒素的(de)(de)(de)(de)(de)試(shi)(shi)管，操(cao)(cao)作(zuo)(zuo)(zuo)(zuo)過程中(zhong)(zhong)(zhong)避(bi)(bi)免(mian)任何細(xi)胞(bao)刺激，收集血液(ye)(ye)(ye)后(hou)，3000轉離心(xin)(xin)10分鐘(zhong)將血清(qing)(qing)和(he)(he)(he)紅細(xi)胞(bao)迅速(su)小心(xin)(xin)地分離。2.  血漿：EDTA、檸檬酸鹽或肝素抗凝。3000轉離心(xin)(xin)30分鐘(zhong)取(qu)(qu)上(shang)(shang)清(qing)(qing)。3.  細(xi)胞(bao)上(shang)(shang)清(qing)(qing)液(ye)(ye)(ye)：3000轉離心(xin)(xin)10分鐘(zhong)去除(chu)顆粒和(he)(he)(he)聚合物(wu)。4.  組(zu)(zu)織勻(yun)(yun)(yun)漿：將組(zu)(zu)織加(jia)(jia)入(ru)適量(liang)生(sheng)理鹽水(shui)搗碎。3000轉離心(xin)(xin)10分鐘(zhong)取(qu)(qu)上(shang)(shang)清(qing)(qing)。5.  保(bao)(bao)存(cun)：如果(guo)(guo)樣(yang)(yang)(yang)本(ben)(ben)(ben)收集后(hou)不(bu)(bu)及時檢測(ce)，請(qing)按(an)一次用(yong)(yong)(yong)(yong)量(liang)分裝，凍存(cun)于(yu)(yu)-20℃，避(bi)(bi)免(mian)反(fan)復(fu)凍融，在(zai)室(shi)溫(wen)(wen)(wen)(wen)下解凍并確保(bao)(bao)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)均勻(yun)(yun)(yun)地充分解凍。大(da)(da)鼠(shu)(shu)雄(xiong)酮(tong)(tong)(tong)(ASD)ELISA試(shi)(shi)劑(ji)(ji)盒(he)(he)自(zi)(zi)備物(wu)品(pin)(pin)(pin)(pin)(pin)1.酶(mei)(mei)標(biao)(biao)儀（450nm）2.高精度(du)(du)(du)(du)加(jia)(jia)樣(yang)(yang)(yang)器及槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3.37℃恒溫(wen)(wen)(wen)(wen)箱(xiang)操(cao)(cao)作(zuo)(zuo)(zuo)(zuo)注意事項1.試(shi)(shi)劑(ji)(ji)盒(he)(he)保(bao)(bao)存(cun)在(zai)2-8℃，使用(yong)(yong)(yong)(yong)前室(shi)溫(wen)(wen)(wen)(wen)平衡20分鐘(zhong)。從冰箱(xiang)取(qu)(qu)出(chu)(chu)的(de)(de)(de)(de)(de)濃(nong)(nong)(nong)縮(suo)洗(xi)(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)會有結(jie)(jie)(jie)晶，這屬于(yu)(yu)正常現象，水(shui)浴加(jia)(jia)熱使結(jie)(jie)(jie)晶完全溶(rong)解后(hou)再(zai)使用(yong)(yong)(yong)(yong)。2.實(shi)驗中(zhong)(zhong)(zhong)不(bu)(bu)用(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)板(ban)(ban)(ban)條應(ying)(ying)(ying)立即放回(hui)(hui)自(zi)(zi)封袋中(zhong)(zhong)(zhong)，密(mi)(mi)封（低溫(wen)(wen)(wen)(wen)干(gan)燥）保(bao)(bao)存(cun)。3.濃(nong)(nong)(nong)度(du)(du)(du)(du)為(wei)0的(de)(de)(de)(de)(de)S0號(hao)標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)即可(ke)視為(wei)陰性(xing)(xing)(xing)(xing)對照或者(zhe)空(kong)(kong)白；按(an)照說(shuo)(shuo)(shuo)明(ming)(ming)書操(cao)(cao)作(zuo)(zuo)(zuo)(zuo)時樣(yang)(yang)(yang)本(ben)(ben)(ben)已經稀(xi)釋(shi)5倍，＊終結(jie)(jie)(jie)果(guo)(guo)乘(cheng)以5才是樣(yang)(yang)(yang)本(ben)(ben)(ben)實(shi)際濃(nong)(nong)(nong)度(du)(du)(du)(du)。4.嚴格按(an)照說(shuo)(shuo)(shuo)明(ming)(ming)書中(zhong)(zhong)(zhong)標(biao)(biao)明(ming)(ming)的(de)(de)(de)(de)(de)時間、加(jia)(jia)液(ye)(ye)(ye)量(liang)及順序進行溫(wen)(wen)(wen)(wen)育操(cao)(cao)作(zuo)(zuo)(zuo)(zuo)。5.所有液(ye)(ye)(ye)體(ti)組(zu)(zu)分使用(yong)(yong)(yong)(yong)前充分搖勻(yun)(yun)(yun)。試(shi)(shi)劑(ji)(ji)盒(he)(he)組(zu)(zu)成名稱96孔(kong)(kong)(kong)(kong)(kong)配置(zhi)(zhi)(zhi)48孔(kong)(kong)(kong)(kong)(kong)配置(zhi)(zhi)(zhi)備注微孔(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)標(biao)(biao)板(ban)(ban)(ban)12孔(kong)(kong)(kong)(kong)(kong)×8條12孔(kong)(kong)(kong)(kong)(kong)×4條無(wu)(wu)(wu)標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管無(wu)(wu)(wu)樣(yang)(yang)(yang)本(ben)(ben)(ben)稀(xi)釋(shi)液(ye)(ye)(ye)6mL3mL無(wu)(wu)(wu)檢測(ce)抗體(ti)-HRP10mL5mL無(wu)(wu)(wu)20×洗(xi)(xi)(xi)(xi)滌(di)(di)緩沖液(ye)(ye)(ye)25mL15mL按(an)說(shuo)(shuo)(shuo)明(ming)(ming)書進行稀(xi)釋(shi)底物(wu)A6mL3mL無(wu)(wu)(wu)底物(wu)B6mL3mL無(wu)(wu)(wu)終止(zhi)液(ye)(ye)(ye)6mL3mL無(wu)(wu)(wu)封板(ban)(ban)(ban)膜2張2張無(wu)(wu)(wu)說(shuo)(shuo)(shuo)明(ming)(ming)書1份1份無(wu)(wu)(wu)自(zi)(zi)封袋1個1個無(wu)(wu)(wu)注：標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)（S0-S5）濃(nong)(nong)(nong)度(du)(du)(du)(du)依次為(wei)：0、30、60、120、240、480 U/ml試(shi)(shi)劑(ji)(ji)的(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)備 20×洗(xi)(xi)(xi)(xi)滌(di)(di)緩沖液(ye)(ye)(ye)的(de)(de)(de)(de)(de)稀(xi)釋(shi)：蒸(zheng)餾(liu)(liu)水(shui)按(an)1：20稀(xi)釋(shi)，即1份的(de)(de)(de)(de)(de)20×洗(xi)(xi)(xi)(xi)滌(di)(di)緩沖液(ye)(ye)(ye)加(jia)(jia)19份的(de)(de)(de)(de)(de)蒸(zheng)餾(liu)(liu)水(shui)。洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)方法(fa)1.手工洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)：甩盡(jin)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)體(ti)，每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)滿(man)洗(xi)(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)，靜置(zhi)(zhi)(zhi)1min后(hou)甩盡(jin)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)體(ti)，在(zai)吸(xi)水(shui)紙(zhi)上(shang)(shang)拍干(gan)，如此洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)5次。2.自(zi)(zi)動洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)機：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)注入(ru)洗(xi)(xi)(xi)(xi)液(ye)(ye)(ye)350μL，浸(jin)泡1min，洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)5次。操(cao)(cao)作(zuo)(zuo)(zuo)(zuo)步(bu)驟1.從室(shi)溫(wen)(wen)(wen)(wen)平衡20min后(hou)的(de)(de)(de)(de)(de)鋁箔袋中(zhong)(zhong)(zhong)取(qu)(qu)出(chu)(chu)所需板(ban)(ban)(ban)條，剩(sheng)余板(ban)(ban)(ban)條用(yong)(yong)(yong)(yong)自(zi)(zi)封袋密(mi)(mi)封放回(hui)(hui)4℃。2.設置(zhi)(zhi)(zhi)標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)和(he)(he)(he)樣(yang)(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)，標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)各(ge)(ge)加(jia)(jia)不(bu)(bu)同濃(nong)(nong)(nong)度(du)(du)(du)(du)的(de)(de)(de)(de)(de)標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)50μL；3.樣(yang)(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)待測(ce)樣(yang)(yang)(yang)本(ben)(ben)(ben)10μL，再(zai)加(jia)(jia)樣(yang)(yang)(yang)本(ben)(ben)(ben)稀(xi)釋(shi)液(ye)(ye)(ye)40μL；空(kong)(kong)白孔(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)。4.除(chu)空(kong)(kong)白孔(kong)(kong)(kong)(kong)(kong)外(wai)，標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)和(he)(he)(he)樣(yang)(yang)(yang)本(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)入(ru)辣(la)根(gen)過氧(yang)(yang)(yang)化(hua)物(wu)酶(mei)(mei)（HRP）標(biao)(biao)記(ji)的(de)(de)(de)(de)(de)檢測(ce)抗體(ti)100μL，用(yong)(yong)(yong)(yong)封板(ban)(ban)(ban)膜封住反(fan)應(ying)(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)，37℃水(shui)浴鍋(guo)或恒溫(wen)(wen)(wen)(wen)箱(xiang)溫(wen)(wen)(wen)(wen)育60min。5.棄去液(ye)(ye)(ye)體(ti)，吸(xi)水(shui)紙(zhi)上(shang)(shang)拍干(gan)，每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)滿(man)洗(xi)(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)，靜置(zhi)(zhi)(zhi)1min，甩去洗(xi)(xi)(xi)(xi)滌(di)(di)液(ye)(ye)(ye)，吸(xi)水(shui)紙(zhi)上(shang)(shang)拍干(gan)，如此重復(fu)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)5次（也可(ke)用(yong)(yong)(yong)(yong)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)機洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)）。6.每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)入(ru)底物(wu)A、B各(ge)(ge)50μL，37℃避(bi)(bi)光孵育15min。7.每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)入(ru)終止(zhi)液(ye)(ye)(ye)50μL，15min內(nei)(nei)，在(zai)450nm波長處測(ce)定各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)OD值(zhi)(zhi)。結(jie)(jie)(jie)果(guo)(guo)判斷 繪制標(biao)(biao)準(zhun)(zhun)(zhun)曲線：在(zai)Excel工作(zuo)(zuo)(zuo)(zuo)表中(zhong)(zhong)(zhong)，以標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)(du)(du)作(zuo)(zuo)(zuo)(zuo)橫坐(zuo)標(biao)(biao)，對應(ying)(ying)(ying)OD值(zhi)(zhi)作(zuo)(zuo)(zuo)(zuo)縱(zong)坐(zuo)標(biao)(biao)，繪制出(chu)(chu)標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)線性(xing)(xing)(xing)(xing)回(hui)(hui)歸曲線，按(an)曲線方程計算各(ge)(ge)樣(yang)(yang)(yang)本(ben)(ben)(ben)濃(nong)(nong)(nong)度(du)(du)(du)(du)值(zhi)(zhi)。大(da)(da)鼠(shu)(shu)雄(xiong)酮(tong)(tong)(tong)(ASD)ELISA試(shi)(shi)劑(ji)(ji)盒(he)(he)試(shi)(shi)劑(ji)(ji)盒(he)(he)性(xing)(xing)(xing)(xing)能1.準(zhun)(zhun)(zhun)確性(xing)(xing)(xing)(xing)：標(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)線性(xing)(xing)(xing)(xing)回(hui)(hui)歸與預(yu)(yu)期濃(nong)(nong)(nong)度(du)(du)(du)(du)相(xiang)(xiang)關系數(shu)R值(zhi)(zhi)，大(da)(da)于(yu)(yu)等于(yu)(yu)0.9900。2.靈敏(min)度(du)(du)(du)(du)：＊低檢測(ce)濃(nong)(nong)(nong)度(du)(du)(du)(du)小于(yu)(yu)1.0 U/ml。3.特異(yi)性(xing)(xing)(xing)(xing)：不(bu)(bu)與其它(ta)可(ke)溶(rong)性(xing)(xing)(xing)(xing)結(jie)(jie)(jie)構類似物(wu)交叉反(fan)應(ying)(ying)(ying)。4.重復(fu)性(xing)(xing)(xing)(xing)：板(ban)(ban)(ban)內(nei)(nei)、板(ban)(ban)(ban)間變異(yi)系數(shu)均小于(yu)(yu)15%。5.貯藏(zang)：2-8℃，避(bi)(bi)光防潮保(bao)(bao)存(cun)。6.有效期：6個月(yue)免(mian)責聲明(ming)(ming)1.試(shi)(shi)劑(ji)(ji)盒(he)(he)僅供研究使用(yong)(yong)(yong)(yong)，不(bu)(bu)得用(yong)(yong)(yong)(yong)于(yu)(yu)臨床實(shi)驗或人體(ti)實(shi)驗，否(fou)則所產生(sheng)的(de)(de)(de)(de)(de)一切(qie)后(hou)果(guo)(guo)，由實(shi)驗者(zhe)承擔，本(ben)(ben)(ben)公司(si)概不(bu)(bu)負責。2.嚴格按(an)照說(shuo)(shuo)(shuo)明(ming)(ming)書操(cao)(cao)作(zuo)(zuo)(zuo)(zuo)，實(shi)驗者(zhe)違反(fan)說(shuo)(shuo)(shuo)明(ming)(ming)書操(cao)(cao)作(zuo)(zuo)(zuo)(zuo)，后(hou)果(guo)(guo)由實(shi)驗者(zhe)承擔。 大(da)(da)鼠(shu)(shu)雄(xiong)酮(tong)(tong)(tong)(ASD)ELISA試(shi)(shi)劑(ji)(ji)盒(he)(he) 

大(da)(da)鼠組(zu)(zu)氨(an)(an)(an)酸(suan)(Histamin)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he) 大(da)(da)鼠組(zu)(zu)氨(an)(an)(an)酸(suan)(Histamin)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)檢(jian)測原(yuan)理(li)試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)采用(yong)(yong)(yong)雙抗(kang)體(ti)一步(bu)夾心(xin)(xin)法(fa)酶(mei)(mei)聯免疫吸(xi)附試(shi)(shi)(shi)驗(yan)(yan)（ELISA）。往(wang)預先包被活性(xing)(xing)(xing)氧(yang)簇（ROS）抗(kang)體(ti)的(de)(de)(de)(de)包被微孔(kong)(kong)(kong)(kong)(kong)中(zhong)，依次加(jia)(jia)(jia)(jia)入標(biao)本(ben)(ben)、標(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)、HRP標(biao)記的(de)(de)(de)(de)檢(jian)測抗(kang)體(ti)，經過(guo)溫(wen)(wen)育并(bing)(bing)徹底洗(xi)(xi)(xi)(xi)滌。用(yong)(yong)(yong)底物(wu)(wu)TMB顯(xian)色，TMB在過(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)酶(mei)(mei)的(de)(de)(de)(de)催化(hua)(hua)(hua)下轉(zhuan)(zhuan)(zhuan)化(hua)(hua)(hua)成(cheng)藍(lan)色，并(bing)(bing)在酸(suan)的(de)(de)(de)(de)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)下轉(zhuan)(zhuan)(zhuan)化(hua)(hua)(hua)成(cheng)＊終(zhong)(zhong)的(de)(de)(de)(de)黃(huang)色。顏色的(de)(de)(de)(de)深淺(qian)和(he)(he)(he)樣(yang)(yang)品(pin)(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)活性(xing)(xing)(xing)氧(yang)簇（ROS）呈(cheng)正(zheng)相關。用(yong)(yong)(yong)酶(mei)(mei)標(biao)儀在450nm 波長(chang)下測定吸(xi)光(guang)度(du)（OD 值），計(ji)算(suan)(suan)樣(yang)(yang)品(pin)(pin)(pin)(pin)活性(xing)(xing)(xing)。大(da)(da)鼠組(zu)(zu)氨(an)(an)(an)酸(suan)(Histamin)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)樣(yang)(yang)品(pin)(pin)(pin)(pin)收(shou)(shou)(shou)集、處理(li)及保存方(fang)法(fa)1.  血(xue)(xue)清：使(shi)(shi)用(yong)(yong)(yong)不(bu)含熱(re)原(yuan)和(he)(he)(he)內(nei)毒素的(de)(de)(de)(de)試(shi)(shi)(shi)管，操(cao)(cao)作(zuo)(zuo)(zuo)過(guo)程中(zhong)避(bi)免任何細胞(bao)刺(ci)激，收(shou)(shou)(shou)集血(xue)(xue)液(ye)(ye)(ye)后，3000轉(zhuan)(zhuan)(zhuan)離(li)心(xin)(xin)10分(fen)(fen)(fen)(fen)鐘將血(xue)(xue)清和(he)(he)(he)紅細胞(bao)迅速小(xiao)心(xin)(xin)地分(fen)(fen)(fen)(fen)離(li)。2.  血(xue)(xue)漿(jiang)：EDTA、檸檬(meng)酸(suan)鹽或肝(gan)素抗(kang)凝。3000轉(zhuan)(zhuan)(zhuan)離(li)心(xin)(xin)30分(fen)(fen)(fen)(fen)鐘取上(shang)(shang)清。3.  細胞(bao)上(shang)(shang)清液(ye)(ye)(ye)：3000轉(zhuan)(zhuan)(zhuan)離(li)心(xin)(xin)10分(fen)(fen)(fen)(fen)鐘去除顆粒和(he)(he)(he)聚合物(wu)(wu)。4.  組(zu)(zu)織(zhi)勻漿(jiang)：將組(zu)(zu)織(zhi)加(jia)(jia)(jia)(jia)入適量生理(li)鹽水(shui)搗(dao)碎(sui)。3000轉(zhuan)(zhuan)(zhuan)離(li)心(xin)(xin)10分(fen)(fen)(fen)(fen)鐘取上(shang)(shang)清。5.  保存：如(ru)(ru)果(guo)樣(yang)(yang)本(ben)(ben)收(shou)(shou)(shou)集后不(bu)及時(shi)(shi)檢(jian)測，請按一次用(yong)(yong)(yong)量分(fen)(fen)(fen)(fen)裝(zhuang)，凍(dong)(dong)存于(yu)-20℃，避(bi)免反復(fu)凍(dong)(dong)融，在室溫(wen)(wen)下解凍(dong)(dong)并(bing)(bing)確保樣(yang)(yang)品(pin)(pin)(pin)(pin)均(jun)勻地充分(fen)(fen)(fen)(fen)解凍(dong)(dong)。大(da)(da)鼠組(zu)(zu)氨(an)(an)(an)酸(suan)(Histamin)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)自備(bei)物(wu)(wu)品(pin)(pin)(pin)(pin)1.酶(mei)(mei)標(biao)儀（450nm）2.高精度(du)加(jia)(jia)(jia)(jia)樣(yang)(yang)器及槍(qiang)頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3.37℃恒(heng)溫(wen)(wen)箱(xiang)操(cao)(cao)作(zuo)(zuo)(zuo)注(zhu)(zhu)意事項(xiang)1.試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)保存在2-8℃，使(shi)(shi)用(yong)(yong)(yong)前室溫(wen)(wen)平(ping)衡20分(fen)(fen)(fen)(fen)鐘。從(cong)冰箱(xiang)取出的(de)(de)(de)(de)濃(nong)(nong)縮洗(xi)(xi)(xi)(xi)滌液(ye)(ye)(ye)會有(you)(you)結(jie)晶，這屬于(yu)正(zheng)常現象，水(shui)浴(yu)加(jia)(jia)(jia)(jia)熱(re)使(shi)(shi)結(jie)晶完全溶解后再(zai)使(shi)(shi)用(yong)(yong)(yong)。2.實(shi)(shi)驗(yan)(yan)中(zhong)不(bu)用(yong)(yong)(yong)的(de)(de)(de)(de)板(ban)(ban)(ban)(ban)(ban)條(tiao)(tiao)應(ying)立即(ji)(ji)放(fang)回(hui)自封(feng)袋(dai)中(zhong)，密封(feng)（低溫(wen)(wen)干(gan)燥(zao)）保存。3.濃(nong)(nong)度(du)為(wei)(wei)0的(de)(de)(de)(de)S0號標(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)即(ji)(ji)可(ke)視為(wei)(wei)陰性(xing)(xing)(xing)對照(zhao)或者空白；按照(zhao)說(shuo)(shuo)(shuo)明(ming)(ming)書(shu)操(cao)(cao)作(zuo)(zuo)(zuo)時(shi)(shi)樣(yang)(yang)本(ben)(ben)已經稀(xi)釋(shi)5倍，＊終(zhong)(zhong)結(jie)果(guo)乘(cheng)以5才是樣(yang)(yang)本(ben)(ben)實(shi)(shi)際(ji)濃(nong)(nong)度(du)。4.嚴格按照(zhao)說(shuo)(shuo)(shuo)明(ming)(ming)書(shu)中(zhong)標(biao)明(ming)(ming)的(de)(de)(de)(de)時(shi)(shi)間(jian)、加(jia)(jia)(jia)(jia)液(ye)(ye)(ye)量及順(shun)序進行(xing)溫(wen)(wen)育操(cao)(cao)作(zuo)(zuo)(zuo)。5.所有(you)(you)液(ye)(ye)(ye)體(ti)組(zu)(zu)分(fen)(fen)(fen)(fen)使(shi)(shi)用(yong)(yong)(yong)前充分(fen)(fen)(fen)(fen)搖(yao)勻。試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)組(zu)(zu)成(cheng)名稱96孔(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)(zhi)48孔(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)(zhi)備(bei)注(zhu)(zhu)微孔(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)標(biao)板(ban)(ban)(ban)(ban)(ban)12孔(kong)(kong)(kong)(kong)(kong)×8條(tiao)(tiao)12孔(kong)(kong)(kong)(kong)(kong)×4條(tiao)(tiao)無標(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管無樣(yang)(yang)本(ben)(ben)稀(xi)釋(shi)液(ye)(ye)(ye)6mL3mL無檢(jian)測抗(kang)體(ti)-HRP10mL5mL無20×洗(xi)(xi)(xi)(xi)滌緩(huan)沖液(ye)(ye)(ye)25mL15mL按說(shuo)(shuo)(shuo)明(ming)(ming)書(shu)進行(xing)稀(xi)釋(shi)底物(wu)(wu)A6mL3mL無底物(wu)(wu)B6mL3mL無終(zhong)(zhong)止液(ye)(ye)(ye)6mL3mL無封(feng)板(ban)(ban)(ban)(ban)(ban)膜(mo)2張(zhang)2張(zhang)無說(shuo)(shuo)(shuo)明(ming)(ming)書(shu)1份(fen)1份(fen)無自封(feng)袋(dai)1個(ge)1個(ge)無注(zhu)(zhu)：標(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)（S0-S5）濃(nong)(nong)度(du)依次為(wei)(wei)：0、30、60、120、240、480 U/ml試(shi)(shi)(shi)劑(ji)(ji)的(de)(de)(de)(de)準(zhun)(zhun)(zhun)備(bei) 20×洗(xi)(xi)(xi)(xi)滌緩(huan)沖液(ye)(ye)(ye)的(de)(de)(de)(de)稀(xi)釋(shi)：蒸餾水(shui)按1：20稀(xi)釋(shi)，即(ji)(ji)1份(fen)的(de)(de)(de)(de)20×洗(xi)(xi)(xi)(xi)滌緩(huan)沖液(ye)(ye)(ye)加(jia)(jia)(jia)(jia)19份(fen)的(de)(de)(de)(de)蒸餾水(shui)。洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)方(fang)法(fa)1.手工洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)：甩(shuai)盡孔(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)體(ti)，每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)(xi)滌液(ye)(ye)(ye)，靜置(zhi)(zhi)1min后甩(shuai)盡孔(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)體(ti)，在吸(xi)水(shui)紙(zhi)上(shang)(shang)拍(pai)干(gan)，如(ru)(ru)此洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)5次。2.自動洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)機(ji)：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)注(zhu)(zhu)入洗(xi)(xi)(xi)(xi)液(ye)(ye)(ye)350μL，浸泡1min，洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)5次。操(cao)(cao)作(zuo)(zuo)(zuo)步(bu)驟1.從(cong)室溫(wen)(wen)平(ping)衡20min后的(de)(de)(de)(de)鋁(lv)箔袋(dai)中(zhong)取出所需板(ban)(ban)(ban)(ban)(ban)條(tiao)(tiao)，剩余板(ban)(ban)(ban)(ban)(ban)條(tiao)(tiao)用(yong)(yong)(yong)自封(feng)袋(dai)密封(feng)放(fang)回(hui)4℃。2.設置(zhi)(zhi)標(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)和(he)(he)(he)樣(yang)(yang)本(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)，標(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)各(ge)加(jia)(jia)(jia)(jia)不(bu)同濃(nong)(nong)度(du)的(de)(de)(de)(de)標(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)50μL；3.樣(yang)(yang)本(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)待測樣(yang)(yang)本(ben)(ben)10μL，再(zai)加(jia)(jia)(jia)(jia)樣(yang)(yang)本(ben)(ben)稀(xi)釋(shi)液(ye)(ye)(ye)40μL；空白孔(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)(jia)(jia)(jia)。4.除空白孔(kong)(kong)(kong)(kong)(kong)外，標(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)和(he)(he)(he)樣(yang)(yang)本(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)中(zhong)每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入辣根(gen)過(guo)氧(yang)化(hua)(hua)(hua)物(wu)(wu)酶(mei)(mei)（HRP）標(biao)記的(de)(de)(de)(de)檢(jian)測抗(kang)體(ti)100μL，用(yong)(yong)(yong)封(feng)板(ban)(ban)(ban)(ban)(ban)膜(mo)封(feng)住反應(ying)孔(kong)(kong)(kong)(kong)(kong)，37℃水(shui)浴(yu)鍋或恒(heng)溫(wen)(wen)箱(xiang)溫(wen)(wen)育60min。5.棄去液(ye)(ye)(ye)體(ti)，吸(xi)水(shui)紙(zhi)上(shang)(shang)拍(pai)干(gan)，每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)(xi)滌液(ye)(ye)(ye)，靜置(zhi)(zhi)1min，甩(shuai)去洗(xi)(xi)(xi)(xi)滌液(ye)(ye)(ye)，吸(xi)水(shui)紙(zhi)上(shang)(shang)拍(pai)干(gan)，如(ru)(ru)此重(zhong)復(fu)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)5次（也可(ke)用(yong)(yong)(yong)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)機(ji)洗(xi)(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)）。6.每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入底物(wu)(wu)A、B各(ge)50μL，37℃避(bi)光(guang)孵育15min。7.每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入終(zhong)(zhong)止液(ye)(ye)(ye)50μL，15min內(nei)，在450nm波長(chang)處測定各(ge)孔(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)OD值。結(jie)果(guo)判斷(duan) 繪制(zhi)標(biao)準(zhun)(zhun)(zhun)曲線(xian)：在Excel工作(zuo)(zuo)(zuo)表中(zhong)，以標(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)作(zuo)(zuo)(zuo)橫坐標(biao)，對應(ying)OD值作(zuo)(zuo)(zuo)縱坐標(biao)，繪制(zhi)出標(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)線(xian)性(xing)(xing)(xing)回(hui)歸曲線(xian)，按曲線(xian)方(fang)程計(ji)算(suan)(suan)各(ge)樣(yang)(yang)本(ben)(ben)濃(nong)(nong)度(du)值。大(da)(da)鼠組(zu)(zu)氨(an)(an)(an)酸(suan)(Histamin)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)性(xing)(xing)(xing)能(neng)1.準(zhun)(zhun)(zhun)確性(xing)(xing)(xing)：標(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)線(xian)性(xing)(xing)(xing)回(hui)歸與預期濃(nong)(nong)度(du)相關系(xi)數R值，大(da)(da)于(yu)等于(yu)0.9900。2.靈(ling)敏度(du)：＊低檢(jian)測濃(nong)(nong)度(du)小(xiao)于(yu)1.0 U/ml。3.特異性(xing)(xing)(xing)：不(bu)與其(qi)它(ta)可(ke)溶性(xing)(xing)(xing)結(jie)構類似物(wu)(wu)交叉反應(ying)。4.重(zhong)復(fu)性(xing)(xing)(xing)：板(ban)(ban)(ban)(ban)(ban)內(nei)、板(ban)(ban)(ban)(ban)(ban)間(jian)變(bian)異系(xi)數均(jun)小(xiao)于(yu)15%。5.貯藏：2-8℃，避(bi)光(guang)防(fang)潮(chao)保存。6.有(you)(you)效期：6個(ge)月免責聲明(ming)(ming)1.試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)僅(jin)供(gong)研究使(shi)(shi)用(yong)(yong)(yong)，不(bu)得(de)用(yong)(yong)(yong)于(yu)臨床實(shi)(shi)驗(yan)(yan)或人(ren)體(ti)實(shi)(shi)驗(yan)(yan)，否則(ze)所產生的(de)(de)(de)(de)一切后果(guo)，由實(shi)(shi)驗(yan)(yan)者承(cheng)擔，本(ben)(ben)公(gong)司概不(bu)負責。2.嚴格按照(zhao)說(shuo)(shuo)(shuo)明(ming)(ming)書(shu)操(cao)(cao)作(zuo)(zuo)(zuo)，實(shi)(shi)驗(yan)(yan)者違反說(shuo)(shuo)(shuo)明(ming)(ming)書(shu)操(cao)(cao)作(zuo)(zuo)(zuo)，后果(guo)由實(shi)(shi)驗(yan)(yan)者承(cheng)擔。 大(da)(da)鼠組(zu)(zu)氨(an)(an)(an)酸(suan)(Histamin)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he) 

大(da)(da)鼠（Rat）活(huo)性(xing)(xing)(xing)氧簇(cu)（ROS）ELISA檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)試(shi)劑(ji)盒大(da)(da)鼠（Rat）活(huo)性(xing)(xing)(xing)氧簇(cu)（ROS）ELISA檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)試(shi)劑(ji)盒檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)原理(li)試(shi)劑(ji)盒采(cai)用(yong)(yong)雙(shuang)抗(kang)體(ti)(ti)一(yi)步夾心(xin)法(fa)酶聯免(mian)疫吸(xi)附試(shi)驗(yan)(yan)（ELISA）。往預先包(bao)被活(huo)性(xing)(xing)(xing)氧簇(cu)（ROS）抗(kang)體(ti)(ti)的(de)(de)(de)(de)包(bao)被微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中，依次(ci)(ci)加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)標(biao)(biao)(biao)本(ben)(ben)、標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)、HRP標(biao)(biao)(biao)記(ji)的(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)抗(kang)體(ti)(ti)，經過溫育并(bing)(bing)徹(che)底洗(xi)(xi)(xi)滌(di)。用(yong)(yong)底物(wu)TMB顯(xian)色(se)(se)(se)，TMB在(zai)(zai)(zai)過氧化(hua)物(wu)酶的(de)(de)(de)(de)催化(hua)下(xia)轉(zhuan)化(hua)成(cheng)藍色(se)(se)(se)，并(bing)(bing)在(zai)(zai)(zai)酸(suan)的(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)下(xia)轉(zhuan)化(hua)成(cheng)＊終(zhong)(zhong)的(de)(de)(de)(de)黃色(se)(se)(se)。顏色(se)(se)(se)的(de)(de)(de)(de)深淺和樣(yang)品(pin)(pin)(pin)中的(de)(de)(de)(de)活(huo)性(xing)(xing)(xing)氧簇(cu)（ROS）呈正(zheng)相關(guan)。用(yong)(yong)酶標(biao)(biao)(biao)儀(yi)在(zai)(zai)(zai)450nm 波(bo)長(chang)下(xia)測(ce)(ce)(ce)(ce)(ce)定(ding)吸(xi)光(guang)度(du)(du)（OD 值(zhi)(zhi)），計(ji)算樣(yang)品(pin)(pin)(pin)活(huo)性(xing)(xing)(xing)。大(da)(da)鼠（Rat）活(huo)性(xing)(xing)(xing)氧簇(cu)（ROS）ELISA檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)試(shi)劑(ji)盒樣(yang)品(pin)(pin)(pin)收集、處理(li)及(ji)(ji)(ji)保(bao)(bao)存(cun)方(fang)法(fa)1.  血(xue)(xue)清(qing)：使(shi)(shi)(shi)用(yong)(yong)不(bu)(bu)(bu)含熱(re)原和內(nei)(nei)毒素(su)的(de)(de)(de)(de)試(shi)管，操(cao)作(zuo)(zuo)過程中避(bi)免(mian)任何細(xi)胞(bao)(bao)刺激(ji)，收集血(xue)(xue)液(ye)后，3000轉(zhuan)離心(xin)10分鐘(zhong)將(jiang)血(xue)(xue)清(qing)和紅細(xi)胞(bao)(bao)迅速小(xiao)(xiao)心(xin)地(di)分離。2.  血(xue)(xue)漿：EDTA、檸檬酸(suan)鹽或(huo)(huo)肝(gan)素(su)抗(kang)凝。3000轉(zhuan)離心(xin)30分鐘(zhong)取上(shang)清(qing)。3.  細(xi)胞(bao)(bao)上(shang)清(qing)液(ye)：3000轉(zhuan)離心(xin)10分鐘(zhong)去(qu)(qu)除(chu)(chu)顆(ke)粒(li)和聚合物(wu)。4.  組(zu)織(zhi)勻(yun)漿：將(jiang)組(zu)織(zhi)加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)適量(liang)(liang)生理(li)鹽水(shui)(shui)搗(dao)碎(sui)。3000轉(zhuan)離心(xin)10分鐘(zhong)取上(shang)清(qing)。5.  保(bao)(bao)存(cun)：如果(guo)樣(yang)本(ben)(ben)收集后不(bu)(bu)(bu)及(ji)(ji)(ji)時檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)，請按(an)一(yi)次(ci)(ci)用(yong)(yong)量(liang)(liang)分裝，凍存(cun)于-20℃，避(bi)免(mian)反(fan)復(fu)凍融，在(zai)(zai)(zai)室溫下(xia)解凍并(bing)(bing)確保(bao)(bao)樣(yang)品(pin)(pin)(pin)均(jun)勻(yun)地(di)充(chong)(chong)分解凍。大(da)(da)鼠（Rat）活(huo)性(xing)(xing)(xing)氧簇(cu)（ROS）ELISA檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)試(shi)劑(ji)盒自(zi)備物(wu)品(pin)(pin)(pin)1.酶標(biao)(biao)(biao)儀(yi)（450nm）2.高精(jing)度(du)(du)加(jia)(jia)(jia)(jia)樣(yang)器及(ji)(ji)(ji)槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3.37℃恒(heng)溫箱(xiang)操(cao)作(zuo)(zuo)注(zhu)(zhu)意(yi)事項(xiang)1.試(shi)劑(ji)盒保(bao)(bao)存(cun)在(zai)(zai)(zai)2-8℃，使(shi)(shi)(shi)用(yong)(yong)前(qian)(qian)室溫平(ping)衡20分鐘(zhong)。從冰(bing)箱(xiang)取出的(de)(de)(de)(de)濃(nong)(nong)(nong)縮(suo)洗(xi)(xi)(xi)滌(di)液(ye)會(hui)有結晶(jing)，這(zhe)屬于正(zheng)常現象，水(shui)(shui)浴加(jia)(jia)(jia)(jia)熱(re)使(shi)(shi)(shi)結晶(jing)完(wan)全溶(rong)解后再(zai)使(shi)(shi)(shi)用(yong)(yong)。2.實(shi)(shi)(shi)驗(yan)(yan)中不(bu)(bu)(bu)用(yong)(yong)的(de)(de)(de)(de)板(ban)(ban)條(tiao)(tiao)應立即(ji)放回(hui)自(zi)封(feng)(feng)(feng)袋中，密(mi)封(feng)(feng)(feng)（低溫干(gan)燥）保(bao)(bao)存(cun)。3.濃(nong)(nong)(nong)度(du)(du)為(wei)0的(de)(de)(de)(de)S0號(hao)標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)即(ji)可視為(wei)陰性(xing)(xing)(xing)對照(zhao)或(huo)(huo)者(zhe)(zhe)空(kong)白(bai)；按(an)照(zhao)說(shuo)(shuo)明(ming)書(shu)操(cao)作(zuo)(zuo)時樣(yang)本(ben)(ben)已(yi)經稀(xi)(xi)(xi)釋(shi)5倍，＊終(zhong)(zhong)結果(guo)乘以(yi)5才是(shi)樣(yang)本(ben)(ben)實(shi)(shi)(shi)際濃(nong)(nong)(nong)度(du)(du)。4.嚴(yan)格(ge)按(an)照(zhao)說(shuo)(shuo)明(ming)書(shu)中標(biao)(biao)(biao)明(ming)的(de)(de)(de)(de)時間(jian)、加(jia)(jia)(jia)(jia)液(ye)量(liang)(liang)及(ji)(ji)(ji)順(shun)序進行(xing)溫育操(cao)作(zuo)(zuo)。5.所(suo)有液(ye)體(ti)(ti)組(zu)分使(shi)(shi)(shi)用(yong)(yong)前(qian)(qian)充(chong)(chong)分搖勻(yun)。試(shi)劑(ji)盒組(zu)成(cheng)名稱96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置備注(zhu)(zhu)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶標(biao)(biao)(biao)板(ban)(ban)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)(tiao)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)(tiao)無標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)0.3mL*6管0.3mL*6管無樣(yang)本(ben)(ben)稀(xi)(xi)(xi)釋(shi)液(ye)6mL3mL無檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)抗(kang)體(ti)(ti)-HRP10mL5mL無20×洗(xi)(xi)(xi)滌(di)緩沖液(ye)25mL15mL按(an)說(shuo)(shuo)明(ming)書(shu)進行(xing)稀(xi)(xi)(xi)釋(shi)底物(wu)A6mL3mL無底物(wu)B6mL3mL無終(zhong)(zhong)止(zhi)液(ye)6mL3mL無封(feng)(feng)(feng)板(ban)(ban)膜(mo)(mo)2張2張無說(shuo)(shuo)明(ming)書(shu)1份1份無自(zi)封(feng)(feng)(feng)袋1個1個無注(zhu)(zhu)：標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)（S0-S5）濃(nong)(nong)(nong)度(du)(du)依次(ci)(ci)為(wei)：0、30、60、120、240、480 U/ml試(shi)劑(ji)的(de)(de)(de)(de)準(zhun)(zhun)備 20×洗(xi)(xi)(xi)滌(di)緩沖液(ye)的(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)：蒸餾水(shui)(shui)按(an)1：20稀(xi)(xi)(xi)釋(shi)，即(ji)1份的(de)(de)(de)(de)20×洗(xi)(xi)(xi)滌(di)緩沖液(ye)加(jia)(jia)(jia)(jia)19份的(de)(de)(de)(de)蒸餾水(shui)(shui)。洗(xi)(xi)(xi)板(ban)(ban)方(fang)法(fa)1.手工洗(xi)(xi)(xi)板(ban)(ban)：甩盡孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)體(ti)(ti)，每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)滌(di)液(ye)，靜置1min后甩盡孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)體(ti)(ti)，在(zai)(zai)(zai)吸(xi)水(shui)(shui)紙(zhi)上(shang)拍干(gan)，如此洗(xi)(xi)(xi)板(ban)(ban)5次(ci)(ci)。2.自(zi)動洗(xi)(xi)(xi)板(ban)(ban)機(ji)：每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)注(zhu)(zhu)入(ru)(ru)(ru)洗(xi)(xi)(xi)液(ye)350μL，浸泡1min，洗(xi)(xi)(xi)板(ban)(ban)5次(ci)(ci)。操(cao)作(zuo)(zuo)步驟(zou)1.從室溫平(ping)衡20min后的(de)(de)(de)(de)鋁箔袋中取出所(suo)需板(ban)(ban)條(tiao)(tiao)，剩余板(ban)(ban)條(tiao)(tiao)用(yong)(yong)自(zi)封(feng)(feng)(feng)袋密(mi)封(feng)(feng)(feng)放回(hui)4℃。2.設置標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和樣(yang)本(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)各(ge)加(jia)(jia)(jia)(jia)不(bu)(bu)(bu)同濃(nong)(nong)(nong)度(du)(du)的(de)(de)(de)(de)標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)50μL；3.樣(yang)本(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)待(dai)測(ce)(ce)(ce)(ce)(ce)樣(yang)本(ben)(ben)10μL，再(zai)加(jia)(jia)(jia)(jia)樣(yang)本(ben)(ben)稀(xi)(xi)(xi)釋(shi)液(ye)40μL；空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)加(jia)(jia)(jia)(jia)。4.除(chu)(chu)空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)外，標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和樣(yang)本(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)辣根過氧化(hua)物(wu)酶（HRP）標(biao)(biao)(biao)記(ji)的(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)抗(kang)體(ti)(ti)100μL，用(yong)(yong)封(feng)(feng)(feng)板(ban)(ban)膜(mo)(mo)封(feng)(feng)(feng)住反(fan)應孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，37℃水(shui)(shui)浴鍋或(huo)(huo)恒(heng)溫箱(xiang)溫育60min。5.棄去(qu)(qu)液(ye)體(ti)(ti)，吸(xi)水(shui)(shui)紙(zhi)上(shang)拍干(gan)，每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)滌(di)液(ye)，靜置1min，甩去(qu)(qu)洗(xi)(xi)(xi)滌(di)液(ye)，吸(xi)水(shui)(shui)紙(zhi)上(shang)拍干(gan)，如此重復(fu)洗(xi)(xi)(xi)板(ban)(ban)5次(ci)(ci)（也可用(yong)(yong)洗(xi)(xi)(xi)板(ban)(ban)機(ji)洗(xi)(xi)(xi)板(ban)(ban)）。6.每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)底物(wu)A、B各(ge)50μL，37℃避(bi)光(guang)孵育15min。7.每(mei)(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)入(ru)(ru)(ru)終(zhong)(zhong)止(zhi)液(ye)50μL，15min內(nei)(nei)，在(zai)(zai)(zai)450nm波(bo)長(chang)處測(ce)(ce)(ce)(ce)(ce)定(ding)各(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)OD值(zhi)(zhi)。結果(guo)判斷 繪制標(biao)(biao)(biao)準(zhun)(zhun)曲(qu)線(xian)：在(zai)(zai)(zai)Excel工作(zuo)(zuo)表中，以(yi)標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)作(zuo)(zuo)橫坐標(biao)(biao)(biao)，對應OD值(zhi)(zhi)作(zuo)(zuo)縱坐標(biao)(biao)(biao)，繪制出標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)線(xian)性(xing)(xing)(xing)回(hui)歸曲(qu)線(xian)，按(an)曲(qu)線(xian)方(fang)程計(ji)算各(ge)樣(yang)本(ben)(ben)濃(nong)(nong)(nong)度(du)(du)值(zhi)(zhi)。大(da)(da)鼠（Rat）活(huo)性(xing)(xing)(xing)氧簇(cu)（ROS）ELISA檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)試(shi)劑(ji)盒試(shi)劑(ji)盒性(xing)(xing)(xing)能1.準(zhun)(zhun)確性(xing)(xing)(xing)：標(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)線(xian)性(xing)(xing)(xing)回(hui)歸與預期(qi)濃(nong)(nong)(nong)度(du)(du)相關(guan)系(xi)數(shu)R值(zhi)(zhi)，大(da)(da)于等于0.9900。2.靈敏度(du)(du)：＊低檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)濃(nong)(nong)(nong)度(du)(du)小(xiao)(xiao)于1.0 U/ml。3.特異性(xing)(xing)(xing)：不(bu)(bu)(bu)與其它可溶(rong)性(xing)(xing)(xing)結構類似(si)物(wu)交叉反(fan)應。4.重復(fu)性(xing)(xing)(xing)：板(ban)(ban)內(nei)(nei)、板(ban)(ban)間(jian)變(bian)異系(xi)數(shu)均(jun)小(xiao)(xiao)于15%。5.貯藏(zang)：2-8℃，避(bi)光(guang)防潮保(bao)(bao)存(cun)。6.有效期(qi)：6個月免(mian)責(ze)聲明(ming)1.試(shi)劑(ji)盒僅供研究使(shi)(shi)(shi)用(yong)(yong)，不(bu)(bu)(bu)得用(yong)(yong)于臨床實(shi)(shi)(shi)驗(yan)(yan)或(huo)(huo)人體(ti)(ti)實(shi)(shi)(shi)驗(yan)(yan)，否則所(suo)產生的(de)(de)(de)(de)一(yi)切后果(guo)，由實(shi)(shi)(shi)驗(yan)(yan)者(zhe)(zhe)承(cheng)擔，本(ben)(ben)公司概不(bu)(bu)(bu)負責(ze)。2.嚴(yan)格(ge)按(an)照(zhao)說(shuo)(shuo)明(ming)書(shu)操(cao)作(zuo)(zuo)，實(shi)(shi)(shi)驗(yan)(yan)者(zhe)(zhe)違反(fan)說(shuo)(shuo)明(ming)書(shu)操(cao)作(zuo)(zuo)，后果(guo)由實(shi)(shi)(shi)驗(yan)(yan)者(zhe)(zhe)承(cheng)擔。    大(da)(da)鼠（Rat）活(huo)性(xing)(xing)(xing)氧簇(cu)（ROS）ELISA檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)試(shi)劑(ji)盒           

大(da)(da)鼠(shu)Adropin蛋(dan)白(bai)(bai)(AD)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he) 大(da)(da)鼠(shu)Adropin蛋(dan)白(bai)(bai)(AD)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he) 檢(jian)測(ce)(ce)原理(li)試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)采(cai)用(yong)(yong)雙抗體(ti)一(yi)步(bu)夾(jia)心(xin)(xin)法(fa)酶(mei)聯免疫吸附試(shi)(shi)(shi)驗(yan)(yan)（ELISA）。往預(yu)先(xian)包被(bei)活(huo)性(xing)(xing)(xing)氧(yang)(yang)簇（ROS）抗體(ti)的(de)(de)(de)(de)(de)(de)包被(bei)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)，依(yi)次(ci)(ci)加(jia)(jia)入(ru)標(biao)(biao)(biao)(biao)本(ben)(ben)(ben)(ben)、標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)、HRP標(biao)(biao)(biao)(biao)記(ji)的(de)(de)(de)(de)(de)(de)檢(jian)測(ce)(ce)抗體(ti)，經過(guo)溫育(yu)并徹底(di)洗(xi)(xi)(xi)滌(di)。用(yong)(yong)底(di)物(wu)(wu)(wu)(wu)(wu)TMB顯色(se)，TMB在(zai)(zai)(zai)過(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)的(de)(de)(de)(de)(de)(de)催(cui)化(hua)(hua)下(xia)(xia)轉(zhuan)化(hua)(hua)成(cheng)藍色(se)，并在(zai)(zai)(zai)酸的(de)(de)(de)(de)(de)(de)作用(yong)(yong)下(xia)(xia)轉(zhuan)化(hua)(hua)成(cheng)＊終(zhong)的(de)(de)(de)(de)(de)(de)黃(huang)色(se)。顏色(se)的(de)(de)(de)(de)(de)(de)深淺和(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)品(pin)(pin)中(zhong)的(de)(de)(de)(de)(de)(de)活(huo)性(xing)(xing)(xing)氧(yang)(yang)簇（ROS）呈正相關。用(yong)(yong)酶(mei)標(biao)(biao)(biao)(biao)儀在(zai)(zai)(zai)450nm 波(bo)(bo)長(chang)下(xia)(xia)測(ce)(ce)定吸光度(du)(du)(du)（OD 值(zhi)），計算(suan)樣(yang)(yang)(yang)(yang)品(pin)(pin)活(huo)性(xing)(xing)(xing)。大(da)(da)鼠(shu)Adropin蛋(dan)白(bai)(bai)(AD)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he) 樣(yang)(yang)(yang)(yang)品(pin)(pin)收集、處(chu)理(li)及(ji)保(bao)(bao)(bao)存(cun)(cun)方(fang)法(fa)1.  血(xue)清(qing)：使(shi)用(yong)(yong)不(bu)(bu)(bu)含熱原和(he)(he)(he)(he)內(nei)毒素的(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)管，操(cao)作過(guo)程中(zhong)避免任(ren)何(he)細(xi)胞(bao)刺激(ji)，收集血(xue)液(ye)(ye)(ye)(ye)后，3000轉(zhuan)離(li)(li)心(xin)(xin)10分(fen)鐘將(jiang)血(xue)清(qing)和(he)(he)(he)(he)紅細(xi)胞(bao)迅速小(xiao)心(xin)(xin)地分(fen)離(li)(li)。2.  血(xue)漿：EDTA、檸檬酸鹽或(huo)肝素抗凝(ning)。3000轉(zhuan)離(li)(li)心(xin)(xin)30分(fen)鐘取(qu)上(shang)清(qing)。3.  細(xi)胞(bao)上(shang)清(qing)液(ye)(ye)(ye)(ye)：3000轉(zhuan)離(li)(li)心(xin)(xin)10分(fen)鐘去除(chu)顆粒(li)和(he)(he)(he)(he)聚合物(wu)(wu)(wu)(wu)(wu)。4.  組織勻漿：將(jiang)組織加(jia)(jia)入(ru)適量生(sheng)理(li)鹽水搗碎。3000轉(zhuan)離(li)(li)心(xin)(xin)10分(fen)鐘取(qu)上(shang)清(qing)。5.  保(bao)(bao)(bao)存(cun)(cun)：如果(guo)(guo)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)收集后不(bu)(bu)(bu)及(ji)時(shi)檢(jian)測(ce)(ce)，請按(an)(an)(an)(an)一(yi)次(ci)(ci)用(yong)(yong)量分(fen)裝，凍(dong)存(cun)(cun)于(yu)-20℃，避免反(fan)復凍(dong)融，在(zai)(zai)(zai)室(shi)溫下(xia)(xia)解凍(dong)并確(que)(que)保(bao)(bao)(bao)樣(yang)(yang)(yang)(yang)品(pin)(pin)均勻地充(chong)分(fen)解凍(dong)。大(da)(da)鼠(shu)Adropin蛋(dan)白(bai)(bai)(AD)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he) 自(zi)備物(wu)(wu)(wu)(wu)(wu)品(pin)(pin)1.酶(mei)標(biao)(biao)(biao)(biao)儀（450nm）2.高精(jing)度(du)(du)(du)加(jia)(jia)樣(yang)(yang)(yang)(yang)器及(ji)槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3.37℃恒(heng)(heng)溫箱(xiang)操(cao)作注意事項1.試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)保(bao)(bao)(bao)存(cun)(cun)在(zai)(zai)(zai)2-8℃，使(shi)用(yong)(yong)前室(shi)溫平(ping)衡20分(fen)鐘。從(cong)冰箱(xiang)取(qu)出的(de)(de)(de)(de)(de)(de)濃縮洗(xi)(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)會有(you)結(jie)晶，這屬于(yu)正常(chang)現(xian)象，水浴加(jia)(jia)熱使(shi)結(jie)晶完(wan)全溶(rong)解后再使(shi)用(yong)(yong)。2.實(shi)驗(yan)(yan)中(zhong)不(bu)(bu)(bu)用(yong)(yong)的(de)(de)(de)(de)(de)(de)板(ban)(ban)條應(ying)立(li)即放回自(zi)封袋中(zhong)，密封（低溫干燥）保(bao)(bao)(bao)存(cun)(cun)。3.濃度(du)(du)(du)為0的(de)(de)(de)(de)(de)(de)S0號標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)即可視為陰性(xing)(xing)(xing)對照或(huo)者(zhe)空(kong)(kong)(kong)白(bai)(bai)；按(an)(an)(an)(an)照說(shuo)明(ming)(ming)書(shu)(shu)操(cao)作時(shi)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)已經稀釋(shi)5倍，＊終(zhong)結(jie)果(guo)(guo)乘以(yi)5才是(shi)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)實(shi)際濃度(du)(du)(du)。4.嚴格(ge)按(an)(an)(an)(an)照說(shuo)明(ming)(ming)書(shu)(shu)中(zhong)標(biao)(biao)(biao)(biao)明(ming)(ming)的(de)(de)(de)(de)(de)(de)時(shi)間(jian)、加(jia)(jia)液(ye)(ye)(ye)(ye)量及(ji)順(shun)序進行溫育(yu)操(cao)作。5.所有(you)液(ye)(ye)(ye)(ye)體(ti)組分(fen)使(shi)用(yong)(yong)前充(chong)分(fen)搖勻。試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)組成(cheng)名(ming)稱96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)(zhi)48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)(zhi)備注微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)標(biao)(biao)(biao)(biao)板(ban)(ban)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條無(wu)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)0.3mL*6管0.3mL*6管無(wu)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)稀釋(shi)液(ye)(ye)(ye)(ye)6mL3mL無(wu)檢(jian)測(ce)(ce)抗體(ti)-HRP10mL5mL無(wu)20×洗(xi)(xi)(xi)滌(di)緩(huan)沖液(ye)(ye)(ye)(ye)25mL15mL按(an)(an)(an)(an)說(shuo)明(ming)(ming)書(shu)(shu)進行稀釋(shi)底(di)物(wu)(wu)(wu)(wu)(wu)A6mL3mL無(wu)底(di)物(wu)(wu)(wu)(wu)(wu)B6mL3mL無(wu)終(zhong)止液(ye)(ye)(ye)(ye)6mL3mL無(wu)封板(ban)(ban)膜2張2張無(wu)說(shuo)明(ming)(ming)書(shu)(shu)1份1份無(wu)自(zi)封袋1個1個無(wu)注：標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)（S0-S5）濃度(du)(du)(du)依(yi)次(ci)(ci)為：0、30、60、120、240、480 U/ml試(shi)(shi)(shi)劑(ji)(ji)的(de)(de)(de)(de)(de)(de)準(zhun)(zhun)(zhun)備 20×洗(xi)(xi)(xi)滌(di)緩(huan)沖液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)稀釋(shi)：蒸(zheng)餾水按(an)(an)(an)(an)1：20稀釋(shi)，即1份的(de)(de)(de)(de)(de)(de)20×洗(xi)(xi)(xi)滌(di)緩(huan)沖液(ye)(ye)(ye)(ye)加(jia)(jia)19份的(de)(de)(de)(de)(de)(de)蒸(zheng)餾水。洗(xi)(xi)(xi)板(ban)(ban)方(fang)法(fa)1.手工(gong)洗(xi)(xi)(xi)板(ban)(ban)：甩(shuai)(shuai)盡孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)體(ti)，每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)滿(man)洗(xi)(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)，靜(jing)置(zhi)(zhi)1min后甩(shuai)(shuai)盡孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)(ye)(ye)(ye)體(ti)，在(zai)(zai)(zai)吸水紙(zhi)上(shang)拍(pai)干，如此(ci)洗(xi)(xi)(xi)板(ban)(ban)5次(ci)(ci)。2.自(zi)動洗(xi)(xi)(xi)板(ban)(ban)機(ji)：每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)注入(ru)洗(xi)(xi)(xi)液(ye)(ye)(ye)(ye)350μL，浸泡1min，洗(xi)(xi)(xi)板(ban)(ban)5次(ci)(ci)。操(cao)作步(bu)驟1.從(cong)室(shi)溫平(ping)衡20min后的(de)(de)(de)(de)(de)(de)鋁箔袋中(zhong)取(qu)出所需板(ban)(ban)條，剩余板(ban)(ban)條用(yong)(yong)自(zi)封袋密封放回4℃。2.設置(zhi)(zhi)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)各(ge)(ge)加(jia)(jia)不(bu)(bu)(bu)同濃度(du)(du)(du)的(de)(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)50μL；3.樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)待測(ce)(ce)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)10μL，再加(jia)(jia)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)稀釋(shi)液(ye)(ye)(ye)(ye)40μL；空(kong)(kong)(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)(bu)加(jia)(jia)。4.除(chu)空(kong)(kong)(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)外，標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)入(ru)辣(la)根(gen)過(guo)氧(yang)(yang)化(hua)(hua)物(wu)(wu)(wu)(wu)(wu)酶(mei)（HRP）標(biao)(biao)(biao)(biao)記(ji)的(de)(de)(de)(de)(de)(de)檢(jian)測(ce)(ce)抗體(ti)100μL，用(yong)(yong)封板(ban)(ban)膜封住反(fan)應(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)，37℃水浴鍋(guo)或(huo)恒(heng)(heng)溫箱(xiang)溫育(yu)60min。5.棄去液(ye)(ye)(ye)(ye)體(ti)，吸水紙(zhi)上(shang)拍(pai)干，每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)滿(man)洗(xi)(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)，靜(jing)置(zhi)(zhi)1min，甩(shuai)(shuai)去洗(xi)(xi)(xi)滌(di)液(ye)(ye)(ye)(ye)，吸水紙(zhi)上(shang)拍(pai)干，如此(ci)重復洗(xi)(xi)(xi)板(ban)(ban)5次(ci)(ci)（也可用(yong)(yong)洗(xi)(xi)(xi)板(ban)(ban)機(ji)洗(xi)(xi)(xi)板(ban)(ban)）。6.每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)入(ru)底(di)物(wu)(wu)(wu)(wu)(wu)A、B各(ge)(ge)50μL，37℃避光孵育(yu)15min。7.每(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)入(ru)終(zhong)止液(ye)(ye)(ye)(ye)50μL，15min內(nei)，在(zai)(zai)(zai)450nm波(bo)(bo)長(chang)處(chu)測(ce)(ce)定各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)(de)OD值(zhi)。結(jie)果(guo)(guo)判斷 繪制(zhi)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)(qu)線(xian)：在(zai)(zai)(zai)Excel工(gong)作表中(zhong)，以(yi)標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)濃度(du)(du)(du)作橫坐標(biao)(biao)(biao)(biao)，對應(ying)OD值(zhi)作縱坐標(biao)(biao)(biao)(biao)，繪制(zhi)出標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)線(xian)性(xing)(xing)(xing)回歸(gui)曲(qu)(qu)線(xian)，按(an)(an)(an)(an)曲(qu)(qu)線(xian)方(fang)程計算(suan)各(ge)(ge)樣(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)濃度(du)(du)(du)值(zhi)。大(da)(da)鼠(shu)Adropin蛋(dan)白(bai)(bai)(AD)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he) 試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)性(xing)(xing)(xing)能(neng)1.準(zhun)(zhun)(zhun)確(que)(que)性(xing)(xing)(xing)：標(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)線(xian)性(xing)(xing)(xing)回歸(gui)與(yu)預(yu)期濃度(du)(du)(du)相關系數(shu)R值(zhi)，大(da)(da)于(yu)等于(yu)0.9900。2.靈敏度(du)(du)(du)：＊低檢(jian)測(ce)(ce)濃度(du)(du)(du)小(xiao)于(yu)1.0 U/ml。3.特異(yi)(yi)性(xing)(xing)(xing)：不(bu)(bu)(bu)與(yu)其它(ta)可溶(rong)性(xing)(xing)(xing)結(jie)構類(lei)似物(wu)(wu)(wu)(wu)(wu)交叉反(fan)應(ying)。4.重復性(xing)(xing)(xing)：板(ban)(ban)內(nei)、板(ban)(ban)間(jian)變異(yi)(yi)系數(shu)均小(xiao)于(yu)15%。5.貯藏(zang)：2-8℃，避光防潮(chao)保(bao)(bao)(bao)存(cun)(cun)。6.有(you)效期：6個月(yue)免責聲明(ming)(ming)1.試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he)僅供研究使(shi)用(yong)(yong)，不(bu)(bu)(bu)得用(yong)(yong)于(yu)臨床實(shi)驗(yan)(yan)或(huo)人體(ti)實(shi)驗(yan)(yan)，否則所產生(sheng)的(de)(de)(de)(de)(de)(de)一(yi)切后果(guo)(guo)，由實(shi)驗(yan)(yan)者(zhe)承擔，本(ben)(ben)(ben)(ben)公司概不(bu)(bu)(bu)負(fu)責。2.嚴格(ge)按(an)(an)(an)(an)照說(shuo)明(ming)(ming)書(shu)(shu)操(cao)作，實(shi)驗(yan)(yan)者(zhe)違反(fan)說(shuo)明(ming)(ming)書(shu)(shu)操(cao)作，后果(guo)(guo)由實(shi)驗(yan)(yan)者(zhe)承擔。  大(da)(da)鼠(shu)Adropin蛋(dan)白(bai)(bai)(AD)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)(he)(he) 

大(da)(da)鼠(shu)蛋(dan)(dan)(dan)白酪(lao)(lao)氨(an)酸激(ji)(ji)酶(mei)(mei)(mei)2(PTK2)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he) 大(da)(da)鼠(shu)蛋(dan)(dan)(dan)白酪(lao)(lao)氨(an)酸激(ji)(ji)酶(mei)(mei)(mei)2(PTK2)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he) 檢測(ce)(ce)(ce)(ce)原(yuan)理試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)采用(yong)(yong)(yong)雙抗(kang)體(ti)(ti)(ti)一步(bu)夾心(xin)法(fa)酶(mei)(mei)(mei)聯(lian)免疫吸(xi)(xi)附(fu)試(shi)(shi)(shi)驗(yan)（ELISA）。往預(yu)先包(bao)(bao)被(bei)活(huo)性(xing)氧簇（ROS）抗(kang)體(ti)(ti)(ti)的(de)(de)(de)(de)包(bao)(bao)被(bei)微(wei)孔(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)，依次(ci)(ci)(ci)加(jia)入(ru)標(biao)(biao)(biao)(biao)(biao)本(ben)(ben)、標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)檢測(ce)(ce)(ce)(ce)抗(kang)體(ti)(ti)(ti)，經過(guo)溫(wen)(wen)(wen)育(yu)并徹(che)底洗(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)。用(yong)(yong)(yong)底物(wu)(wu)TMB顯(xian)色(se)(se)，TMB在(zai)(zai)過(guo)氧化(hua)(hua)物(wu)(wu)酶(mei)(mei)(mei)的(de)(de)(de)(de)催化(hua)(hua)下(xia)轉(zhuan)化(hua)(hua)成藍色(se)(se)，并在(zai)(zai)酸的(de)(de)(de)(de)作(zuo)用(yong)(yong)(yong)下(xia)轉(zhuan)化(hua)(hua)成＊終的(de)(de)(de)(de)黃色(se)(se)。顏色(se)(se)的(de)(de)(de)(de)深淺和(he)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)的(de)(de)(de)(de)活(huo)性(xing)氧簇（ROS）呈正相關。用(yong)(yong)(yong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)儀在(zai)(zai)450nm 波長(chang)(chang)下(xia)測(ce)(ce)(ce)(ce)定吸(xi)(xi)光(guang)度(du)（OD 值(zhi)），計(ji)算樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)活(huo)性(xing)。大(da)(da)鼠(shu)蛋(dan)(dan)(dan)白酪(lao)(lao)氨(an)酸激(ji)(ji)酶(mei)(mei)(mei)2(PTK2)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he) 樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)收集(ji)、處(chu)理及保(bao)存(cun)(cun)(cun)方(fang)法(fa)1.  血(xue)清(qing)：使用(yong)(yong)(yong)不(bu)含熱(re)原(yuan)和(he)內毒素(su)的(de)(de)(de)(de)試(shi)(shi)(shi)管(guan)，操(cao)(cao)作(zuo)過(guo)程中(zhong)(zhong)避(bi)(bi)免任何細胞刺激(ji)(ji)，收集(ji)血(xue)液(ye)(ye)后，3000轉(zhuan)離(li)心(xin)10分(fen)鐘(zhong)將血(xue)清(qing)和(he)紅細胞迅速小(xiao)心(xin)地分(fen)離(li)。2.  血(xue)漿(jiang)：EDTA、檸檬酸鹽或(huo)肝素(su)抗(kang)凝。3000轉(zhuan)離(li)心(xin)30分(fen)鐘(zhong)取(qu)上(shang)(shang)清(qing)。3.  細胞上(shang)(shang)清(qing)液(ye)(ye)：3000轉(zhuan)離(li)心(xin)10分(fen)鐘(zhong)去除顆粒和(he)聚(ju)合(he)物(wu)(wu)。4.  組織(zhi)勻漿(jiang)：將組織(zhi)加(jia)入(ru)適量生(sheng)理鹽水(shui)(shui)搗(dao)碎。3000轉(zhuan)離(li)心(xin)10分(fen)鐘(zhong)取(qu)上(shang)(shang)清(qing)。5.  保(bao)存(cun)(cun)(cun)：如果(guo)(guo)樣(yang)(yang)(yang)本(ben)(ben)收集(ji)后不(bu)及時(shi)檢測(ce)(ce)(ce)(ce)，請按一次(ci)(ci)(ci)用(yong)(yong)(yong)量分(fen)裝，凍存(cun)(cun)(cun)于(yu)(yu)-20℃，避(bi)(bi)免反復(fu)凍融，在(zai)(zai)室(shi)溫(wen)(wen)(wen)下(xia)解(jie)凍并確保(bao)樣(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)均勻地充分(fen)解(jie)凍。大(da)(da)鼠(shu)蛋(dan)(dan)(dan)白酪(lao)(lao)氨(an)酸激(ji)(ji)酶(mei)(mei)(mei)2(PTK2)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he) 自備(bei)物(wu)(wu)品(pin)(pin)(pin)(pin)(pin)1.酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)儀（450nm）2.高精度(du)加(jia)樣(yang)(yang)(yang)器(qi)及槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3.37℃恒(heng)溫(wen)(wen)(wen)箱操(cao)(cao)作(zuo)注意事項1.試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)保(bao)存(cun)(cun)(cun)在(zai)(zai)2-8℃，使用(yong)(yong)(yong)前(qian)室(shi)溫(wen)(wen)(wen)平(ping)衡20分(fen)鐘(zhong)。從冰箱取(qu)出(chu)的(de)(de)(de)(de)濃(nong)縮洗(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)會有(you)結晶，這屬(shu)于(yu)(yu)正常現象，水(shui)(shui)浴(yu)加(jia)熱(re)使結晶完全溶解(jie)后再(zai)使用(yong)(yong)(yong)。2.實(shi)(shi)驗(yan)中(zhong)(zhong)不(bu)用(yong)(yong)(yong)的(de)(de)(de)(de)板(ban)(ban)(ban)條(tiao)應(ying)(ying)立即(ji)放(fang)回自封(feng)袋(dai)(dai)中(zhong)(zhong)，密(mi)(mi)封(feng)（低(di)溫(wen)(wen)(wen)干燥）保(bao)存(cun)(cun)(cun)。3.濃(nong)度(du)為0的(de)(de)(de)(de)S0號標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)即(ji)可(ke)視為陰性(xing)對照或(huo)者(zhe)(zhe)空(kong)白；按照說(shuo)明(ming)(ming)書操(cao)(cao)作(zuo)時(shi)樣(yang)(yang)(yang)本(ben)(ben)已(yi)經稀(xi)(xi)(xi)釋(shi)(shi)5倍，＊終結果(guo)(guo)乘以5才是樣(yang)(yang)(yang)本(ben)(ben)實(shi)(shi)際(ji)濃(nong)度(du)。4.嚴格(ge)按照說(shuo)明(ming)(ming)書中(zhong)(zhong)標(biao)(biao)(biao)(biao)(biao)明(ming)(ming)的(de)(de)(de)(de)時(shi)間、加(jia)液(ye)(ye)量及順序(xu)進行溫(wen)(wen)(wen)育(yu)操(cao)(cao)作(zuo)。5.所(suo)有(you)液(ye)(ye)體(ti)(ti)(ti)組分(fen)使用(yong)(yong)(yong)前(qian)充分(fen)搖勻。試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)組成名稱96孔(kong)(kong)(kong)(kong)(kong)配(pei)(pei)置(zhi)(zhi)48孔(kong)(kong)(kong)(kong)(kong)配(pei)(pei)置(zhi)(zhi)備(bei)注微(wei)孔(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)(mei)標(biao)(biao)(biao)(biao)(biao)板(ban)(ban)(ban)12孔(kong)(kong)(kong)(kong)(kong)×8條(tiao)12孔(kong)(kong)(kong)(kong)(kong)×4條(tiao)無(wu)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)0.3mL*6管(guan)0.3mL*6管(guan)無(wu)樣(yang)(yang)(yang)本(ben)(ben)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)6mL3mL無(wu)檢測(ce)(ce)(ce)(ce)抗(kang)體(ti)(ti)(ti)-HRP10mL5mL無(wu)20×洗(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)緩沖液(ye)(ye)25mL15mL按說(shuo)明(ming)(ming)書進行稀(xi)(xi)(xi)釋(shi)(shi)底物(wu)(wu)A6mL3mL無(wu)底物(wu)(wu)B6mL3mL無(wu)終止液(ye)(ye)6mL3mL無(wu)封(feng)板(ban)(ban)(ban)膜2張2張無(wu)說(shuo)明(ming)(ming)書1份(fen)(fen)1份(fen)(fen)無(wu)自封(feng)袋(dai)(dai)1個1個無(wu)注：標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)（S0-S5）濃(nong)度(du)依次(ci)(ci)(ci)為：0、30、60、120、240、480 U/ml試(shi)(shi)(shi)劑(ji)(ji)的(de)(de)(de)(de)準(zhun)(zhun)(zhun)備(bei) 20×洗(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)緩沖液(ye)(ye)的(de)(de)(de)(de)稀(xi)(xi)(xi)釋(shi)(shi)：蒸(zheng)餾水(shui)(shui)按1：20稀(xi)(xi)(xi)釋(shi)(shi)，即(ji)1份(fen)(fen)的(de)(de)(de)(de)20×洗(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)緩沖液(ye)(ye)加(jia)19份(fen)(fen)的(de)(de)(de)(de)蒸(zheng)餾水(shui)(shui)。洗(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)方(fang)法(fa)1.手工洗(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)：甩盡孔(kong)(kong)(kong)(kong)(kong)內液(ye)(ye)體(ti)(ti)(ti)，每(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)滿洗(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)，靜置(zhi)(zhi)1min后甩盡孔(kong)(kong)(kong)(kong)(kong)內液(ye)(ye)體(ti)(ti)(ti)，在(zai)(zai)吸(xi)(xi)水(shui)(shui)紙上(shang)(shang)拍(pai)干，如此(ci)洗(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)5次(ci)(ci)(ci)。2.自動洗(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)機(ji)：每(mei)孔(kong)(kong)(kong)(kong)(kong)注入(ru)洗(xi)(xi)(xi)(xi)(xi)液(ye)(ye)350μL，浸泡1min，洗(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)5次(ci)(ci)(ci)。操(cao)(cao)作(zuo)步(bu)驟1.從室(shi)溫(wen)(wen)(wen)平(ping)衡20min后的(de)(de)(de)(de)鋁箔袋(dai)(dai)中(zhong)(zhong)取(qu)出(chu)所(suo)需(xu)板(ban)(ban)(ban)條(tiao)，剩余板(ban)(ban)(ban)條(tiao)用(yong)(yong)(yong)自封(feng)袋(dai)(dai)密(mi)(mi)封(feng)放(fang)回4℃。2.設置(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)和(he)樣(yang)(yang)(yang)本(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)，標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)各(ge)(ge)加(jia)不(bu)同濃(nong)度(du)的(de)(de)(de)(de)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)50μL；3.樣(yang)(yang)(yang)本(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)先加(jia)待測(ce)(ce)(ce)(ce)樣(yang)(yang)(yang)本(ben)(ben)10μL，再(zai)加(jia)樣(yang)(yang)(yang)本(ben)(ben)稀(xi)(xi)(xi)釋(shi)(shi)液(ye)(ye)40μL；空(kong)白孔(kong)(kong)(kong)(kong)(kong)不(bu)加(jia)。4.除空(kong)白孔(kong)(kong)(kong)(kong)(kong)外(wai)，標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)和(he)樣(yang)(yang)(yang)本(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)每(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)入(ru)辣根過(guo)氧化(hua)(hua)物(wu)(wu)酶(mei)(mei)(mei)（HRP）標(biao)(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)檢測(ce)(ce)(ce)(ce)抗(kang)體(ti)(ti)(ti)100μL，用(yong)(yong)(yong)封(feng)板(ban)(ban)(ban)膜封(feng)住反應(ying)(ying)孔(kong)(kong)(kong)(kong)(kong)，37℃水(shui)(shui)浴(yu)鍋或(huo)恒(heng)溫(wen)(wen)(wen)箱溫(wen)(wen)(wen)育(yu)60min。5.棄(qi)去液(ye)(ye)體(ti)(ti)(ti)，吸(xi)(xi)水(shui)(shui)紙上(shang)(shang)拍(pai)干，每(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)滿洗(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)，靜置(zhi)(zhi)1min，甩去洗(xi)(xi)(xi)(xi)(xi)滌(di)(di)(di)液(ye)(ye)，吸(xi)(xi)水(shui)(shui)紙上(shang)(shang)拍(pai)干，如此(ci)重復(fu)洗(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)5次(ci)(ci)(ci)（也(ye)可(ke)用(yong)(yong)(yong)洗(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)機(ji)洗(xi)(xi)(xi)(xi)(xi)板(ban)(ban)(ban)）。6.每(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)入(ru)底物(wu)(wu)A、B各(ge)(ge)50μL，37℃避(bi)(bi)光(guang)孵育(yu)15min。7.每(mei)孔(kong)(kong)(kong)(kong)(kong)加(jia)入(ru)終止液(ye)(ye)50μL，15min內，在(zai)(zai)450nm波長(chang)(chang)處(chu)測(ce)(ce)(ce)(ce)定各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)OD值(zhi)。結果(guo)(guo)判斷(duan) 繪(hui)制(zhi)(zhi)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)曲(qu)線：在(zai)(zai)Excel工作(zuo)表中(zhong)(zhong)，以標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)濃(nong)度(du)作(zuo)橫坐(zuo)標(biao)(biao)(biao)(biao)(biao)，對應(ying)(ying)OD值(zhi)作(zuo)縱坐(zuo)標(biao)(biao)(biao)(biao)(biao)，繪(hui)制(zhi)(zhi)出(chu)標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)線性(xing)回歸曲(qu)線，按曲(qu)線方(fang)程計(ji)算各(ge)(ge)樣(yang)(yang)(yang)本(ben)(ben)濃(nong)度(du)值(zhi)。大(da)(da)鼠(shu)蛋(dan)(dan)(dan)白酪(lao)(lao)氨(an)酸激(ji)(ji)酶(mei)(mei)(mei)2(PTK2)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he) 試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)性(xing)能1.準(zhun)(zhun)(zhun)確性(xing)：標(biao)(biao)(biao)(biao)(biao)準(zhun)(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)線性(xing)回歸與預(yu)期(qi)濃(nong)度(du)相關系數(shu)R值(zhi)，大(da)(da)于(yu)(yu)等于(yu)(yu)0.9900。2.靈敏(min)度(du)：＊低(di)檢測(ce)(ce)(ce)(ce)濃(nong)度(du)小(xiao)于(yu)(yu)1.0 U/ml。3.特異性(xing)：不(bu)與其(qi)它可(ke)溶性(xing)結構類似物(wu)(wu)交叉反應(ying)(ying)。4.重復(fu)性(xing)：板(ban)(ban)(ban)內、板(ban)(ban)(ban)間變異系數(shu)均小(xiao)于(yu)(yu)15%。5.貯藏：2-8℃，避(bi)(bi)光(guang)防(fang)潮(chao)保(bao)存(cun)(cun)(cun)。6.有(you)效期(qi)：6個月免責聲明(ming)(ming)1.試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he)僅供(gong)研究使用(yong)(yong)(yong)，不(bu)得用(yong)(yong)(yong)于(yu)(yu)臨床實(shi)(shi)驗(yan)或(huo)人體(ti)(ti)(ti)實(shi)(shi)驗(yan)，否則所(suo)產生(sheng)的(de)(de)(de)(de)一切后果(guo)(guo)，由(you)實(shi)(shi)驗(yan)者(zhe)(zhe)承擔，本(ben)(ben)公司概不(bu)負責。2.嚴格(ge)按照說(shuo)明(ming)(ming)書操(cao)(cao)作(zuo)，實(shi)(shi)驗(yan)者(zhe)(zhe)違反說(shuo)明(ming)(ming)書操(cao)(cao)作(zuo)，后果(guo)(guo)由(you)實(shi)(shi)驗(yan)者(zhe)(zhe)承擔。 大(da)(da)鼠(shu)蛋(dan)(dan)(dan)白酪(lao)(lao)氨(an)酸激(ji)(ji)酶(mei)(mei)(mei)2(PTK2)ELISA試(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)(he) 

大(da)鼠(shu)單(dan)胺氧(yang)(yang)(yang)(yang)化(hua)酶(mei)(mei)A(MAOA)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he) 大(da)鼠(shu)單(dan)胺氧(yang)(yang)(yang)(yang)化(hua)酶(mei)(mei)A(MAOA)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he) 檢(jian)(jian)測(ce)(ce)原(yuan)理(li)試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)采用(yong)(yong)(yong)(yong)雙抗(kang)(kang)體(ti)(ti)一步(bu)夾(jia)心(xin)法酶(mei)(mei)聯免疫(yi)吸(xi)(xi)附試(shi)(shi)(shi)(shi)(shi)驗（ELISA）。往預先包(bao)被(bei)活性(xing)(xing)(xing)氧(yang)(yang)(yang)(yang)簇(cu)（ROS）抗(kang)(kang)體(ti)(ti)的(de)(de)(de)(de)包(bao)被(bei)微(wei)孔(kong)(kong)(kong)(kong)(kong)中(zhong)，依(yi)次(ci)(ci)加(jia)(jia)(jia)(jia)(jia)入(ru)標(biao)本、標(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)、HRP標(biao)記(ji)的(de)(de)(de)(de)檢(jian)(jian)測(ce)(ce)抗(kang)(kang)體(ti)(ti)，經過溫(wen)(wen)(wen)(wen)育(yu)并(bing)徹底洗(xi)(xi)(xi)滌。用(yong)(yong)(yong)(yong)底物(wu)(wu)(wu)TMB顯色，TMB在過氧(yang)(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)酶(mei)(mei)的(de)(de)(de)(de)催化(hua)下轉化(hua)成(cheng)藍色，并(bing)在酸的(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)下轉化(hua)成(cheng)＊終的(de)(de)(de)(de)黃色。顏色的(de)(de)(de)(de)深淺和(he)(he)(he)樣(yang)品(pin)(pin)(pin)中(zhong)的(de)(de)(de)(de)活性(xing)(xing)(xing)氧(yang)(yang)(yang)(yang)簇(cu)（ROS）呈正相(xiang)關。用(yong)(yong)(yong)(yong)酶(mei)(mei)標(biao)儀(yi)在450nm 波長下測(ce)(ce)定吸(xi)(xi)光(guang)(guang)度(du)(du)（OD 值），計算樣(yang)品(pin)(pin)(pin)活性(xing)(xing)(xing)。大(da)鼠(shu)單(dan)胺氧(yang)(yang)(yang)(yang)化(hua)酶(mei)(mei)A(MAOA)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he) 樣(yang)品(pin)(pin)(pin)收(shou)集、處理(li)及(ji)保(bao)存方(fang)法1.  血清：使(shi)用(yong)(yong)(yong)(yong)不(bu)(bu)含熱原(yuan)和(he)(he)(he)內(nei)(nei)毒素的(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)管(guan)，操(cao)作(zuo)(zuo)(zuo)(zuo)過程(cheng)中(zhong)避(bi)免任何細胞刺激(ji)，收(shou)集血液(ye)(ye)(ye)(ye)(ye)后(hou)，3000轉離(li)(li)(li)(li)心(xin)10分(fen)(fen)鐘將血清和(he)(he)(he)紅細胞迅速小心(xin)地(di)分(fen)(fen)離(li)(li)(li)(li)。2.  血漿：EDTA、檸檬酸鹽(yan)或(huo)(huo)肝(gan)素抗(kang)(kang)凝。3000轉離(li)(li)(li)(li)心(xin)30分(fen)(fen)鐘取(qu)上(shang)(shang)清。3.  細胞上(shang)(shang)清液(ye)(ye)(ye)(ye)(ye)：3000轉離(li)(li)(li)(li)心(xin)10分(fen)(fen)鐘去除顆粒(li)和(he)(he)(he)聚合物(wu)(wu)(wu)。4.  組織(zhi)(zhi)勻漿：將組織(zhi)(zhi)加(jia)(jia)(jia)(jia)(jia)入(ru)適量(liang)(liang)生理(li)鹽(yan)水(shui)(shui)(shui)搗碎。3000轉離(li)(li)(li)(li)心(xin)10分(fen)(fen)鐘取(qu)上(shang)(shang)清。5.  保(bao)存：如(ru)果樣(yang)本收(shou)集后(hou)不(bu)(bu)及(ji)時檢(jian)(jian)測(ce)(ce)，請(qing)按(an)一次(ci)(ci)用(yong)(yong)(yong)(yong)量(liang)(liang)分(fen)(fen)裝，凍(dong)存于-20℃，避(bi)免反(fan)復(fu)凍(dong)融(rong)，在室溫(wen)(wen)(wen)(wen)下解凍(dong)并(bing)確保(bao)樣(yang)品(pin)(pin)(pin)均勻地(di)充分(fen)(fen)解凍(dong)。大(da)鼠(shu)單(dan)胺氧(yang)(yang)(yang)(yang)化(hua)酶(mei)(mei)A(MAOA)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he) 自備(bei)(bei)物(wu)(wu)(wu)品(pin)(pin)(pin)1.酶(mei)(mei)標(biao)儀(yi)（450nm）2.高精度(du)(du)加(jia)(jia)(jia)(jia)(jia)樣(yang)器及(ji)槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3.37℃恒溫(wen)(wen)(wen)(wen)箱操(cao)作(zuo)(zuo)(zuo)(zuo)注意事項(xiang)1.試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)保(bao)存在2-8℃，使(shi)用(yong)(yong)(yong)(yong)前(qian)室溫(wen)(wen)(wen)(wen)平衡20分(fen)(fen)鐘。從(cong)冰箱取(qu)出(chu)(chu)的(de)(de)(de)(de)濃(nong)(nong)(nong)縮洗(xi)(xi)(xi)滌液(ye)(ye)(ye)(ye)(ye)會有(you)(you)結晶(jing)，這屬于正常(chang)現象，水(shui)(shui)(shui)浴(yu)加(jia)(jia)(jia)(jia)(jia)熱使(shi)結晶(jing)完全溶解后(hou)再(zai)使(shi)用(yong)(yong)(yong)(yong)。2.實(shi)(shi)驗中(zhong)不(bu)(bu)用(yong)(yong)(yong)(yong)的(de)(de)(de)(de)板(ban)(ban)(ban)(ban)(ban)條(tiao)應(ying)立(li)即(ji)放(fang)回自封袋中(zhong)，密(mi)封（低(di)溫(wen)(wen)(wen)(wen)干燥）保(bao)存。3.濃(nong)(nong)(nong)度(du)(du)為0的(de)(de)(de)(de)S0號標(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)即(ji)可(ke)視為陰(yin)性(xing)(xing)(xing)對(dui)(dui)照(zhao)或(huo)(huo)者空(kong)白(bai)；按(an)照(zhao)說(shuo)(shuo)明(ming)(ming)書(shu)(shu)操(cao)作(zuo)(zuo)(zuo)(zuo)時樣(yang)本已經稀(xi)(xi)釋5倍，＊終結果乘以5才是樣(yang)本實(shi)(shi)際濃(nong)(nong)(nong)度(du)(du)。4.嚴格(ge)按(an)照(zhao)說(shuo)(shuo)明(ming)(ming)書(shu)(shu)中(zhong)標(biao)明(ming)(ming)的(de)(de)(de)(de)時間、加(jia)(jia)(jia)(jia)(jia)液(ye)(ye)(ye)(ye)(ye)量(liang)(liang)及(ji)順序進(jin)行溫(wen)(wen)(wen)(wen)育(yu)操(cao)作(zuo)(zuo)(zuo)(zuo)。5.所(suo)有(you)(you)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)組分(fen)(fen)使(shi)用(yong)(yong)(yong)(yong)前(qian)充分(fen)(fen)搖(yao)勻。試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)組成(cheng)名(ming)稱96孔(kong)(kong)(kong)(kong)(kong)配置48孔(kong)(kong)(kong)(kong)(kong)配置備(bei)(bei)注微(wei)孔(kong)(kong)(kong)(kong)(kong)酶(mei)(mei)標(biao)板(ban)(ban)(ban)(ban)(ban)12孔(kong)(kong)(kong)(kong)(kong)×8條(tiao)12孔(kong)(kong)(kong)(kong)(kong)×4條(tiao)無(wu)(wu)(wu)(wu)標(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)0.3mL*6管(guan)0.3mL*6管(guan)無(wu)(wu)(wu)(wu)樣(yang)本稀(xi)(xi)釋液(ye)(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)(wu)(wu)檢(jian)(jian)測(ce)(ce)抗(kang)(kang)體(ti)(ti)-HRP10mL5mL無(wu)(wu)(wu)(wu)20×洗(xi)(xi)(xi)滌緩沖(chong)液(ye)(ye)(ye)(ye)(ye)25mL15mL按(an)說(shuo)(shuo)明(ming)(ming)書(shu)(shu)進(jin)行稀(xi)(xi)釋底物(wu)(wu)(wu)A6mL3mL無(wu)(wu)(wu)(wu)底物(wu)(wu)(wu)B6mL3mL無(wu)(wu)(wu)(wu)終止(zhi)液(ye)(ye)(ye)(ye)(ye)6mL3mL無(wu)(wu)(wu)(wu)封板(ban)(ban)(ban)(ban)(ban)膜(mo)2張2張無(wu)(wu)(wu)(wu)說(shuo)(shuo)明(ming)(ming)書(shu)(shu)1份1份無(wu)(wu)(wu)(wu)自封袋1個(ge)1個(ge)無(wu)(wu)(wu)(wu)注：標(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)（S0-S5）濃(nong)(nong)(nong)度(du)(du)依(yi)次(ci)(ci)為：0、30、60、120、240、480 U/ml試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)的(de)(de)(de)(de)準(zhun)(zhun)(zhun)(zhun)備(bei)(bei) 20×洗(xi)(xi)(xi)滌緩沖(chong)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)稀(xi)(xi)釋：蒸(zheng)餾水(shui)(shui)(shui)按(an)1：20稀(xi)(xi)釋，即(ji)1份的(de)(de)(de)(de)20×洗(xi)(xi)(xi)滌緩沖(chong)液(ye)(ye)(ye)(ye)(ye)加(jia)(jia)(jia)(jia)(jia)19份的(de)(de)(de)(de)蒸(zheng)餾水(shui)(shui)(shui)。洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)方(fang)法1.手工(gong)(gong)洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)：甩盡(jin)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，每孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)滌液(ye)(ye)(ye)(ye)(ye)，靜置1min后(hou)甩盡(jin)孔(kong)(kong)(kong)(kong)(kong)內(nei)(nei)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，在吸(xi)(xi)水(shui)(shui)(shui)紙上(shang)(shang)拍(pai)干，如(ru)此洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)5次(ci)(ci)。2.自動洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)機(ji)(ji)：每孔(kong)(kong)(kong)(kong)(kong)注入(ru)洗(xi)(xi)(xi)液(ye)(ye)(ye)(ye)(ye)350μL，浸泡1min，洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)5次(ci)(ci)。操(cao)作(zuo)(zuo)(zuo)(zuo)步(bu)驟1.從(cong)室溫(wen)(wen)(wen)(wen)平衡20min后(hou)的(de)(de)(de)(de)鋁箔(bo)袋中(zhong)取(qu)出(chu)(chu)所(suo)需板(ban)(ban)(ban)(ban)(ban)條(tiao)，剩余板(ban)(ban)(ban)(ban)(ban)條(tiao)用(yong)(yong)(yong)(yong)自封袋密(mi)封放(fang)回4℃。2.設置標(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)和(he)(he)(he)樣(yang)本孔(kong)(kong)(kong)(kong)(kong)，標(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)各加(jia)(jia)(jia)(jia)(jia)不(bu)(bu)同濃(nong)(nong)(nong)度(du)(du)的(de)(de)(de)(de)標(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)50μL；3.樣(yang)本孔(kong)(kong)(kong)(kong)(kong)先加(jia)(jia)(jia)(jia)(jia)待測(ce)(ce)樣(yang)本10μL，再(zai)加(jia)(jia)(jia)(jia)(jia)樣(yang)本稀(xi)(xi)釋液(ye)(ye)(ye)(ye)(ye)40μL；空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)(jia)(jia)。4.除空(kong)白(bai)孔(kong)(kong)(kong)(kong)(kong)外(wai)，標(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)和(he)(he)(he)樣(yang)本孔(kong)(kong)(kong)(kong)(kong)中(zhong)每孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)入(ru)辣(la)根過氧(yang)(yang)(yang)(yang)化(hua)物(wu)(wu)(wu)酶(mei)(mei)（HRP）標(biao)記(ji)的(de)(de)(de)(de)檢(jian)(jian)測(ce)(ce)抗(kang)(kang)體(ti)(ti)100μL，用(yong)(yong)(yong)(yong)封板(ban)(ban)(ban)(ban)(ban)膜(mo)封住反(fan)應(ying)孔(kong)(kong)(kong)(kong)(kong)，37℃水(shui)(shui)(shui)浴(yu)鍋或(huo)(huo)恒溫(wen)(wen)(wen)(wen)箱溫(wen)(wen)(wen)(wen)育(yu)60min。5.棄去液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，吸(xi)(xi)水(shui)(shui)(shui)紙上(shang)(shang)拍(pai)干，每孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)滿洗(xi)(xi)(xi)滌液(ye)(ye)(ye)(ye)(ye)，靜置1min，甩去洗(xi)(xi)(xi)滌液(ye)(ye)(ye)(ye)(ye)，吸(xi)(xi)水(shui)(shui)(shui)紙上(shang)(shang)拍(pai)干，如(ru)此重復(fu)洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)5次(ci)(ci)（也可(ke)用(yong)(yong)(yong)(yong)洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)機(ji)(ji)洗(xi)(xi)(xi)板(ban)(ban)(ban)(ban)(ban)）。6.每孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)入(ru)底物(wu)(wu)(wu)A、B各50μL，37℃避(bi)光(guang)(guang)孵育(yu)15min。7.每孔(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)(jia)(jia)入(ru)終止(zhi)液(ye)(ye)(ye)(ye)(ye)50μL，15min內(nei)(nei)，在450nm波長處測(ce)(ce)定各孔(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)OD值。結果判斷 繪(hui)制標(biao)準(zhun)(zhun)(zhun)(zhun)曲(qu)線(xian)：在Excel工(gong)(gong)作(zuo)(zuo)(zuo)(zuo)表(biao)中(zhong)，以標(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)濃(nong)(nong)(nong)度(du)(du)作(zuo)(zuo)(zuo)(zuo)橫坐標(biao)，對(dui)(dui)應(ying)OD值作(zuo)(zuo)(zuo)(zuo)縱坐標(biao)，繪(hui)制出(chu)(chu)標(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)線(xian)性(xing)(xing)(xing)回歸曲(qu)線(xian)，按(an)曲(qu)線(xian)方(fang)程(cheng)計算各樣(yang)本濃(nong)(nong)(nong)度(du)(du)值。大(da)鼠(shu)單(dan)胺氧(yang)(yang)(yang)(yang)化(hua)酶(mei)(mei)A(MAOA)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he) 試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)性(xing)(xing)(xing)能1.準(zhun)(zhun)(zhun)(zhun)確性(xing)(xing)(xing)：標(biao)準(zhun)(zhun)(zhun)(zhun)品(pin)(pin)(pin)線(xian)性(xing)(xing)(xing)回歸與預期濃(nong)(nong)(nong)度(du)(du)相(xiang)關系數(shu)R值，大(da)于等于0.9900。2.靈敏度(du)(du)：＊低(di)檢(jian)(jian)測(ce)(ce)濃(nong)(nong)(nong)度(du)(du)小于1.0 U/ml。3.特異性(xing)(xing)(xing)：不(bu)(bu)與其它可(ke)溶性(xing)(xing)(xing)結構類似物(wu)(wu)(wu)交叉反(fan)應(ying)。4.重復(fu)性(xing)(xing)(xing)：板(ban)(ban)(ban)(ban)(ban)內(nei)(nei)、板(ban)(ban)(ban)(ban)(ban)間變異系數(shu)均小于15%。5.貯藏：2-8℃，避(bi)光(guang)(guang)防潮(chao)保(bao)存。6.有(you)(you)效期：6個(ge)月免責(ze)聲明(ming)(ming)1.試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he)僅供研(yan)究(jiu)使(shi)用(yong)(yong)(yong)(yong)，不(bu)(bu)得用(yong)(yong)(yong)(yong)于臨床實(shi)(shi)驗或(huo)(huo)人體(ti)(ti)實(shi)(shi)驗，否則所(suo)產生的(de)(de)(de)(de)一切后(hou)果，由(you)實(shi)(shi)驗者承擔，本公司概不(bu)(bu)負責(ze)。2.嚴格(ge)按(an)照(zhao)說(shuo)(shuo)明(ming)(ming)書(shu)(shu)操(cao)作(zuo)(zuo)(zuo)(zuo)，實(shi)(shi)驗者違反(fan)說(shuo)(shuo)明(ming)(ming)書(shu)(shu)操(cao)作(zuo)(zuo)(zuo)(zuo)，后(hou)果由(you)實(shi)(shi)驗者承擔。 大(da)鼠(shu)單(dan)胺氧(yang)(yang)(yang)(yang)化(hua)酶(mei)(mei)A(MAOA)ELISA試(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)盒(he)(he) 

大(da)鼠神(shen)(shen)經(jing)(jing)絲(si)蛋(dan)(dan)白(bai)(bai)3(NEF3)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he) 大(da)鼠神(shen)(shen)經(jing)(jing)絲(si)蛋(dan)(dan)白(bai)(bai)3(NEF3)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he) 檢(jian)(jian)測(ce)原(yuan)理試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)采(cai)用(yong)(yong)雙抗(kang)體(ti)一(yi)步(bu)夾心法酶(mei)聯免(mian)疫(yi)吸(xi)附試(shi)(shi)(shi)驗（ELISA）。往預(yu)先(xian)包(bao)被(bei)活(huo)性氧(yang)簇（ROS）抗(kang)體(ti)的(de)(de)(de)(de)(de)包(bao)被(bei)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)，依次(ci)加(jia)(jia)(jia)入標(biao)(biao)(biao)(biao)本(ben)(ben)、標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)、HRP標(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)(de)檢(jian)(jian)測(ce)抗(kang)體(ti)，經(jing)(jing)過(guo)溫(wen)(wen)(wen)育并(bing)徹底洗(xi)(xi)(xi)(xi)滌(di)。用(yong)(yong)底物(wu)TMB顯色(se)，TMB在過(guo)氧(yang)化(hua)(hua)物(wu)酶(mei)的(de)(de)(de)(de)(de)催(cui)化(hua)(hua)下轉(zhuan)(zhuan)化(hua)(hua)成藍色(se)，并(bing)在酸的(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)下轉(zhuan)(zhuan)化(hua)(hua)成＊終(zhong)的(de)(de)(de)(de)(de)黃色(se)。顏(yan)色(se)的(de)(de)(de)(de)(de)深淺(qian)和(he)樣(yang)品(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)活(huo)性氧(yang)簇（ROS）呈正相(xiang)(xiang)關。用(yong)(yong)酶(mei)標(biao)(biao)(biao)(biao)儀在450nm 波(bo)長下測(ce)定吸(xi)光(guang)度(du)(du)(du)(du)（OD 值(zhi)(zhi)），計算(suan)樣(yang)品(pin)(pin)(pin)(pin)(pin)(pin)活(huo)性。大(da)鼠神(shen)(shen)經(jing)(jing)絲(si)蛋(dan)(dan)白(bai)(bai)3(NEF3)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he) 樣(yang)品(pin)(pin)(pin)(pin)(pin)(pin)收集(ji)、處(chu)理及保存(cun)(cun)(cun)方法1.  血(xue)(xue)清(qing)：使(shi)(shi)用(yong)(yong)不(bu)(bu)含熱原(yuan)和(he)內(nei)毒(du)素的(de)(de)(de)(de)(de)試(shi)(shi)(shi)管，操(cao)(cao)作(zuo)(zuo)過(guo)程(cheng)中(zhong)(zhong)(zhong)避免(mian)任何細(xi)胞刺激，收集(ji)血(xue)(xue)液(ye)后(hou)(hou)，3000轉(zhuan)(zhuan)離(li)心10分(fen)(fen)鐘將血(xue)(xue)清(qing)和(he)紅細(xi)胞迅(xun)速小(xiao)心地(di)分(fen)(fen)離(li)。2.  血(xue)(xue)漿：EDTA、檸檬(meng)酸鹽或(huo)肝(gan)素抗(kang)凝。3000轉(zhuan)(zhuan)離(li)心30分(fen)(fen)鐘取上(shang)(shang)(shang)清(qing)。3.  細(xi)胞上(shang)(shang)(shang)清(qing)液(ye)：3000轉(zhuan)(zhuan)離(li)心10分(fen)(fen)鐘去(qu)(qu)除顆粒和(he)聚合物(wu)。4.  組(zu)織勻(yun)漿：將組(zu)織加(jia)(jia)(jia)入適量(liang)(liang)生理鹽水(shui)(shui)搗(dao)碎。3000轉(zhuan)(zhuan)離(li)心10分(fen)(fen)鐘取上(shang)(shang)(shang)清(qing)。5.  保存(cun)(cun)(cun)：如(ru)(ru)果樣(yang)本(ben)(ben)收集(ji)后(hou)(hou)不(bu)(bu)及時(shi)(shi)檢(jian)(jian)測(ce)，請按(an)(an)一(yi)次(ci)用(yong)(yong)量(liang)(liang)分(fen)(fen)裝，凍(dong)存(cun)(cun)(cun)于(yu)-20℃，避免(mian)反(fan)復(fu)凍(dong)融(rong)，在室(shi)溫(wen)(wen)(wen)下解凍(dong)并(bing)確保樣(yang)品(pin)(pin)(pin)(pin)(pin)(pin)均勻(yun)地(di)充分(fen)(fen)解凍(dong)。大(da)鼠神(shen)(shen)經(jing)(jing)絲(si)蛋(dan)(dan)白(bai)(bai)3(NEF3)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he) 自(zi)(zi)備物(wu)品(pin)(pin)(pin)(pin)(pin)(pin)1.酶(mei)標(biao)(biao)(biao)(biao)儀（450nm）2.高(gao)精度(du)(du)(du)(du)加(jia)(jia)(jia)樣(yang)器及槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3.37℃恒溫(wen)(wen)(wen)箱(xiang)操(cao)(cao)作(zuo)(zuo)注(zhu)(zhu)意事項1.試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)保存(cun)(cun)(cun)在2-8℃，使(shi)(shi)用(yong)(yong)前(qian)室(shi)溫(wen)(wen)(wen)平(ping)衡20分(fen)(fen)鐘。從(cong)冰箱(xiang)取出(chu)的(de)(de)(de)(de)(de)濃(nong)(nong)縮(suo)洗(xi)(xi)(xi)(xi)滌(di)液(ye)會有(you)結(jie)(jie)晶(jing)，這屬(shu)于(yu)正常現象，水(shui)(shui)浴加(jia)(jia)(jia)熱使(shi)(shi)結(jie)(jie)晶(jing)完全溶解后(hou)(hou)再使(shi)(shi)用(yong)(yong)。2.實(shi)(shi)驗中(zhong)(zhong)(zhong)不(bu)(bu)用(yong)(yong)的(de)(de)(de)(de)(de)板(ban)(ban)條(tiao)應(ying)立即(ji)放(fang)回自(zi)(zi)封袋(dai)中(zhong)(zhong)(zhong)，密封（低(di)溫(wen)(wen)(wen)干燥）保存(cun)(cun)(cun)。3.濃(nong)(nong)度(du)(du)(du)(du)為(wei)0的(de)(de)(de)(de)(de)S0號標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)即(ji)可視為(wei)陰性對(dui)照(zhao)(zhao)或(huo)者空(kong)白(bai)(bai)；按(an)(an)照(zhao)(zhao)說明(ming)書(shu)(shu)操(cao)(cao)作(zuo)(zuo)時(shi)(shi)樣(yang)本(ben)(ben)已經(jing)(jing)稀釋5倍(bei)，＊終(zhong)結(jie)(jie)果乘(cheng)以(yi)5才是樣(yang)本(ben)(ben)實(shi)(shi)際濃(nong)(nong)度(du)(du)(du)(du)。4.嚴(yan)格按(an)(an)照(zhao)(zhao)說明(ming)書(shu)(shu)中(zhong)(zhong)(zhong)標(biao)(biao)(biao)(biao)明(ming)的(de)(de)(de)(de)(de)時(shi)(shi)間、加(jia)(jia)(jia)液(ye)量(liang)(liang)及順序進行溫(wen)(wen)(wen)育操(cao)(cao)作(zuo)(zuo)。5.所有(you)液(ye)體(ti)組(zu)分(fen)(fen)使(shi)(shi)用(yong)(yong)前(qian)充分(fen)(fen)搖勻(yun)。試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)組(zu)成名(ming)稱96孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)(zhi)48孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)配(pei)置(zhi)(zhi)備注(zhu)(zhu)微孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)酶(mei)標(biao)(biao)(biao)(biao)板(ban)(ban)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×8條(tiao)12孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)×4條(tiao)無(wu)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)0.3mL*6管0.3mL*6管無(wu)樣(yang)本(ben)(ben)稀釋液(ye)6mL3mL無(wu)檢(jian)(jian)測(ce)抗(kang)體(ti)-HRP10mL5mL無(wu)20×洗(xi)(xi)(xi)(xi)滌(di)緩(huan)(huan)沖液(ye)25mL15mL按(an)(an)說明(ming)書(shu)(shu)進行稀釋底物(wu)A6mL3mL無(wu)底物(wu)B6mL3mL無(wu)終(zhong)止液(ye)6mL3mL無(wu)封板(ban)(ban)膜2張2張無(wu)說明(ming)書(shu)(shu)1份1份無(wu)自(zi)(zi)封袋(dai)1個(ge)1個(ge)無(wu)注(zhu)(zhu)：標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)（S0-S5）濃(nong)(nong)度(du)(du)(du)(du)依次(ci)為(wei)：0、30、60、120、240、480 U/ml試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)準(zhun)(zhun)備 20×洗(xi)(xi)(xi)(xi)滌(di)緩(huan)(huan)沖液(ye)的(de)(de)(de)(de)(de)稀釋：蒸餾水(shui)(shui)按(an)(an)1：20稀釋，即(ji)1份的(de)(de)(de)(de)(de)20×洗(xi)(xi)(xi)(xi)滌(di)緩(huan)(huan)沖液(ye)加(jia)(jia)(jia)19份的(de)(de)(de)(de)(de)蒸餾水(shui)(shui)。洗(xi)(xi)(xi)(xi)板(ban)(ban)方法1.手工(gong)洗(xi)(xi)(xi)(xi)板(ban)(ban)：甩盡(jin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)體(ti)，每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)滿洗(xi)(xi)(xi)(xi)滌(di)液(ye)，靜(jing)置(zhi)(zhi)1min后(hou)(hou)甩盡(jin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)內(nei)液(ye)體(ti)，在吸(xi)水(shui)(shui)紙上(shang)(shang)(shang)拍(pai)干，如(ru)(ru)此洗(xi)(xi)(xi)(xi)板(ban)(ban)5次(ci)。2.自(zi)(zi)動洗(xi)(xi)(xi)(xi)板(ban)(ban)機：每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)注(zhu)(zhu)入洗(xi)(xi)(xi)(xi)液(ye)350μL，浸泡1min，洗(xi)(xi)(xi)(xi)板(ban)(ban)5次(ci)。操(cao)(cao)作(zuo)(zuo)步(bu)驟1.從(cong)室(shi)溫(wen)(wen)(wen)平(ping)衡20min后(hou)(hou)的(de)(de)(de)(de)(de)鋁箔袋(dai)中(zhong)(zhong)(zhong)取出(chu)所需板(ban)(ban)條(tiao)，剩余板(ban)(ban)條(tiao)用(yong)(yong)自(zi)(zi)封袋(dai)密封放(fang)回4℃。2.設置(zhi)(zhi)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)樣(yang)本(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)各(ge)(ge)加(jia)(jia)(jia)不(bu)(bu)同濃(nong)(nong)度(du)(du)(du)(du)的(de)(de)(de)(de)(de)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)50μL；3.樣(yang)本(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)先(xian)加(jia)(jia)(jia)待測(ce)樣(yang)本(ben)(ben)10μL，再加(jia)(jia)(jia)樣(yang)本(ben)(ben)稀釋液(ye)40μL；空(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)不(bu)(bu)加(jia)(jia)(jia)。4.除空(kong)白(bai)(bai)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)外，標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)和(he)樣(yang)本(ben)(ben)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)中(zhong)(zhong)(zhong)每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)入辣(la)根(gen)過(guo)氧(yang)化(hua)(hua)物(wu)酶(mei)（HRP）標(biao)(biao)(biao)(biao)記的(de)(de)(de)(de)(de)檢(jian)(jian)測(ce)抗(kang)體(ti)100μL，用(yong)(yong)封板(ban)(ban)膜封住反(fan)應(ying)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)，37℃水(shui)(shui)浴鍋(guo)或(huo)恒溫(wen)(wen)(wen)箱(xiang)溫(wen)(wen)(wen)育60min。5.棄去(qu)(qu)液(ye)體(ti)，吸(xi)水(shui)(shui)紙上(shang)(shang)(shang)拍(pai)干，每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)滿洗(xi)(xi)(xi)(xi)滌(di)液(ye)，靜(jing)置(zhi)(zhi)1min，甩去(qu)(qu)洗(xi)(xi)(xi)(xi)滌(di)液(ye)，吸(xi)水(shui)(shui)紙上(shang)(shang)(shang)拍(pai)干，如(ru)(ru)此重(zhong)復(fu)洗(xi)(xi)(xi)(xi)板(ban)(ban)5次(ci)（也可用(yong)(yong)洗(xi)(xi)(xi)(xi)板(ban)(ban)機洗(xi)(xi)(xi)(xi)板(ban)(ban)）。6.每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)入底物(wu)A、B各(ge)(ge)50μL，37℃避光(guang)孵育15min。7.每(mei)(mei)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)加(jia)(jia)(jia)入終(zhong)止液(ye)50μL，15min內(nei)，在450nm波(bo)長處(chu)測(ce)定各(ge)(ge)孔(kong)(kong)(kong)(kong)(kong)(kong)(kong)的(de)(de)(de)(de)(de)OD值(zhi)(zhi)。結(jie)(jie)果判(pan)斷 繪制(zhi)標(biao)(biao)(biao)(biao)準(zhun)(zhun)曲(qu)線(xian)：在Excel工(gong)作(zuo)(zuo)表(biao)中(zhong)(zhong)(zhong)，以(yi)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)濃(nong)(nong)度(du)(du)(du)(du)作(zuo)(zuo)橫(heng)坐(zuo)標(biao)(biao)(biao)(biao)，對(dui)應(ying)OD值(zhi)(zhi)作(zuo)(zuo)縱坐(zuo)標(biao)(biao)(biao)(biao)，繪制(zhi)出(chu)標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)線(xian)性回歸曲(qu)線(xian)，按(an)(an)曲(qu)線(xian)方程(cheng)計算(suan)各(ge)(ge)樣(yang)本(ben)(ben)濃(nong)(nong)度(du)(du)(du)(du)值(zhi)(zhi)。大(da)鼠神(shen)(shen)經(jing)(jing)絲(si)蛋(dan)(dan)白(bai)(bai)3(NEF3)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he) 試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)性能1.準(zhun)(zhun)確性：標(biao)(biao)(biao)(biao)準(zhun)(zhun)品(pin)(pin)(pin)(pin)(pin)(pin)線(xian)性回歸與(yu)預(yu)期(qi)濃(nong)(nong)度(du)(du)(du)(du)相(xiang)(xiang)關系(xi)數R值(zhi)(zhi)，大(da)于(yu)等于(yu)0.9900。2.靈敏度(du)(du)(du)(du)：＊低(di)檢(jian)(jian)測(ce)濃(nong)(nong)度(du)(du)(du)(du)小(xiao)于(yu)1.0 U/ml。3.特異性：不(bu)(bu)與(yu)其它可溶性結(jie)(jie)構(gou)類似物(wu)交叉反(fan)應(ying)。4.重(zhong)復(fu)性：板(ban)(ban)內(nei)、板(ban)(ban)間變異系(xi)數均小(xiao)于(yu)15%。5.貯(zhu)藏：2-8℃，避光(guang)防潮保存(cun)(cun)(cun)。6.有(you)效期(qi)：6個(ge)月免(mian)責(ze)聲(sheng)明(ming)1.試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)僅(jin)供研究使(shi)(shi)用(yong)(yong)，不(bu)(bu)得用(yong)(yong)于(yu)臨床實(shi)(shi)驗或(huo)人體(ti)實(shi)(shi)驗，否則(ze)所產(chan)生的(de)(de)(de)(de)(de)一(yi)切后(hou)(hou)果，由實(shi)(shi)驗者承擔，本(ben)(ben)公司(si)概不(bu)(bu)負(fu)責(ze)。2.嚴(yan)格按(an)(an)照(zhao)(zhao)說明(ming)書(shu)(shu)操(cao)(cao)作(zuo)(zuo)，實(shi)(shi)驗者違反(fan)說明(ming)書(shu)(shu)操(cao)(cao)作(zuo)(zuo)，后(hou)(hou)果由實(shi)(shi)驗者承擔。 大(da)鼠神(shen)(shen)經(jing)(jing)絲(si)蛋(dan)(dan)白(bai)(bai)3(NEF3)ELISA試(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)盒(he)