驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)（去(qu)(qu)紅細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、無(wu)(wu)(wu)菌(jun)(jun)過(guo)(guo)(guo)(guo)濾(lv)）使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)方法根據具(ju)體(ti)(ti)實驗來(lai)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)。商品簡介 驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)通過(guo)(guo)(guo)(guo)無(wu)(wu)(wu)菌(jun)(jun)采(cai)集(ji)驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)，凝(ning)固(gu)離(li)心后獲得驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。注意(yi)事項(xiang)1、注意(yi)無(wu)(wu)(wu)菌(jun)(jun)操作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)，避免反復(fu)(fu)(fu)(fu)凍融(rong)。2、反復(fu)(fu)(fu)(fu)凍融(rong)可(ke)(ke)(ke)能產生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)沉淀(dian)，可(ke)(ke)(ke)以通過(guo)(guo)(guo)(guo)無(wu)(wu)(wu)菌(jun)(jun)條(tiao)件(jian)下，3500 rpm離(li)心10 min除(chu)(chu)去(qu)(qu)沉淀(dian)。 驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)成(cheng)(cheng)(cheng)(cheng)(cheng)年(nian)健康驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)為(wei)原(yuan)(yuan)(yuan)(yuan)料(liao)加工(gong)而(er)(er)(er)成(cheng)(cheng)(cheng)(cheng)(cheng)，經(jing)無(wu)(wu)(wu)菌(jun)(jun)采(cai)集(ji)、分(fen)(fen)(fen)(fen)(fen)(fen)離(li)、微孔過(guo)(guo)(guo)(guo)濾(lv)分(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)而(er)(er)(er)成(cheng)(cheng)(cheng)(cheng)(cheng)。無(wu)(wu)(wu)支原(yuan)(yuan)(yuan)(yuan)體(ti)(ti)、無(wu)(wu)(wu)噬菌(jun)(jun)體(ti)(ti)，內毒(du)素(su)(su)(su)(su)含(han)(han)(han)(han)(han)量(liang)(liang)(liang)低(di)(di)于(yu)50EU/ml。 驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)由(you)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)去(qu)(qu)除(chu)(chu)纖(xian)(xian)(xian)維(wei)(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)(yuan)而(er)(er)(er)形成(cheng)(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)種(zhong)(zhong)很(hen)復(fu)(fu)(fu)(fu)雜的(de)(de)(de)(de)(de)(de)(de)(de)混(hun)合(he)(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)，其(qi)組(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)雖(sui)大部(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)已知，但還(huan)(huan)有(you)(you)(you)(you)(you)(you)(you)一(yi)(yi)(yi)(yi)部(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)尚(shang)不(bu)(bu)(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，且(qie)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)及(ji)(ji)含(han)(han)(han)(han)(han)量(liang)(liang)(liang)常(chang)隨供(gong)(gong)(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)別、年(nian)齡(ling)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)條(tiao)件(jian)和(he)營(ying)(ying)養(yang)(yang)(yang)條(tiao)件(jian)不(bu)(bu)(bu)(bu)(bu)(bu)同而(er)(er)(er)異。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)中(zhong)(zhong)(zhong)(zhong)不(bu)(bu)(bu)(bu)(bu)(bu)含(han)(han)(han)(han)(han)纖(xian)(xian)(xian)維(wei)(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)(yuan)的(de)(de)(de)(de)(de)(de)(de)(de)膠狀(zhuang)液(ye)(ye)體(ti)(ti),具(ju)有(you)(you)(you)(you)(you)(you)(you)維(wei)(wei)(wei)(wei)(wei)(wei)持(chi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)正(zheng)(zheng)常(chang)粘(zhan)度(du)、酸(suan)(suan)堿度(du)、滲透(tou)(tou)壓(ya)等(deng)(deng)(deng)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。它主(zhu)要(yao)(yao)由(you)水(shui)和(he)各(ge)種(zhong)(zhong)化(hua)(hua)(hua)學(xue)(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)組(zu)成(cheng)(cheng)(cheng)(cheng)(cheng),這(zhe)(zhe)些(xie)化(hua)(hua)(hua)學(xue)(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)包(bao)括白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、α1、α2、β、γ-球(qiu)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai),甘(gan)油(you)三酯,總(zong)(zong)膽(dan)固(gu)醇,谷丙轉(zhuan)氨(an)酶(mei)等(deng)(deng)(deng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)含(han)(han)(han)(han)(han)有(you)(you)(you)(you)(you)(you)(you)各(ge)種(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、多(duo)(duo)肽(tai)(tai)(tai)(tai)(tai)、脂肪、碳水(shui)化(hua)(hua)(hua)合(he)(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)(yin)子(zi)(zi)(zi)(zi)、激(ji)(ji)(ji)素(su)(su)(su)(su)、無(wu)(wu)(wu)機(ji)(ji)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)等(deng)(deng)(deng)，這(zhe)(zhe)些(xie)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)對(dui)(dui)(dui)(dui)促(cu)(cu)進(jin)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)或(huo)(huo)抑制(zhi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)活性(xing)(xing)是(shi)(shi)(shi)達到(dao)(dao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)平(ping)衡(heng)的(de)(de)(de)(de)(de)(de)(de)(de)。概述 驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)指(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)凝(ning)固(gu)后，在(zai)(zai)(zai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)中(zhong)(zhong)(zhong)(zhong)除(chu)(chu)去(qu)(qu)纖(xian)(xian)(xian)維(wei)(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)分(fen)(fen)(fen)(fen)(fen)(fen)離(li)出(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)淡黃色透(tou)(tou)明液(ye)(ye)體(ti)(ti)或(huo)(huo)指(zhi)纖(xian)(xian)(xian)維(wei)(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)已被除(chu)(chu)去(qu)(qu)的(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)，用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)科研(yan)(yan)、診斷試劑生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產等(deng)(deng)(deng)。驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)提(ti)(ti)(ti)供(gong)(gong)(gong)基(ji)(ji)(ji)本(ben)(ben)營(ying)(ying)養(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)、提(ti)(ti)(ti)供(gong)(gong)(gong)激(ji)(ji)(ji)素(su)(su)(su)(su)和(he)各(ge)種(zhong)(zhong)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)(yin)子(zi)(zi)(zi)(zi)、提(ti)(ti)(ti)供(gong)(gong)(gong)結(jie)(jie)(jie)合(he)(he)(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、提(ti)(ti)(ti)供(gong)(gong)(gong)促(cu)(cu)接觸。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)應(ying)保(bao)存在(zai)(zai)(zai)-5℃至-20℃，若(ruo)存放(fang)于(yu)4C時(shi)勿(wu)超(chao)過(guo)(guo)(guo)(guo)一(yi)(yi)(yi)(yi)個月，若(ruo)一(yi)(yi)(yi)(yi)次無(wu)(wu)(wu)法用(yong)(yong)(yong)(yong)(yong)(yong)完一(yi)(yi)(yi)(yi)瓶(ping)，將無(wu)(wu)(wu)菌(jun)(jun)分(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)至恰當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)滅菌(jun)(jun)容器內，再放(fang)回(hui)冷凍。背景 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，指(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)凝(ning)固(gu)后，在(zai)(zai)(zai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)中(zhong)(zhong)(zhong)(zhong)除(chu)(chu)去(qu)(qu)纖(xian)(xian)(xian)維(wei)(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)分(fen)(fen)(fen)(fen)(fen)(fen)離(li)出(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)淡黃色透(tou)(tou)明液(ye)(ye)體(ti)(ti)或(huo)(huo)指(zhi)纖(xian)(xian)(xian)維(wei)(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)已被除(chu)(chu)去(qu)(qu)的(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)。其(qi)主(zhu)要(yao)(yao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)提(ti)(ti)(ti)供(gong)(gong)(gong)基(ji)(ji)(ji)本(ben)(ben)營(ying)(ying)養(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)、提(ti)(ti)(ti)供(gong)(gong)(gong)激(ji)(ji)(ji)素(su)(su)(su)(su)和(he)各(ge)種(zhong)(zhong)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)(yin)子(zi)(zi)(zi)(zi)、提(ti)(ti)(ti)供(gong)(gong)(gong)結(jie)(jie)(jie)合(he)(he)(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、提(ti)(ti)(ti)供(gong)(gong)(gong)促(cu)(cu)接觸和(he)伸展因(yin)(yin)(yin)(yin)子(zi)(zi)(zi)(zi)使(shi)(shi)(shi)(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼壁免受(shou)機(ji)(ji)械損傷、對(dui)(dui)(dui)(dui)培養(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)起(qi)(qi)到(dao)(dao)某些(xie)保(bao)護(hu)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)性(xing)(xing)質(zhi)(zhi)(zhi)驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)由(you)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)去(qu)(qu)除(chu)(chu)纖(xian)(xian)(xian)維(wei)(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)(yuan)而(er)(er)(er)形成(cheng)(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)種(zhong)(zhong)很(hen)復(fu)(fu)(fu)(fu)雜的(de)(de)(de)(de)(de)(de)(de)(de)混(hun)合(he)(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)，其(qi)組(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)雖(sui)大部(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)已知，但還(huan)(huan)有(you)(you)(you)(you)(you)(you)(you)一(yi)(yi)(yi)(yi)部(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)尚(shang)不(bu)(bu)(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，且(qie)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)及(ji)(ji)含(han)(han)(han)(han)(han)量(liang)(liang)(liang)常(chang)隨供(gong)(gong)(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)別、年(nian)齡(ling)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)條(tiao)件(jian)和(he)營(ying)(ying)養(yang)(yang)(yang)條(tiao)件(jian)不(bu)(bu)(bu)(bu)(bu)(bu)同而(er)(er)(er)異。作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)中(zhong)(zhong)(zhong)(zhong)不(bu)(bu)(bu)(bu)(bu)(bu)含(han)(han)(han)(han)(han)纖(xian)(xian)(xian)維(wei)(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)(yuan)的(de)(de)(de)(de)(de)(de)(de)(de)膠狀(zhuang)液(ye)(ye)體(ti)(ti),具(ju)有(you)(you)(you)(you)(you)(you)(you)維(wei)(wei)(wei)(wei)(wei)(wei)持(chi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)正(zheng)(zheng)常(chang)粘(zhan)度(du)、酸(suan)(suan)堿度(du)、滲透(tou)(tou)壓(ya)等(deng)(deng)(deng)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。它主(zhu)要(yao)(yao)由(you)水(shui)和(he)各(ge)種(zhong)(zhong)化(hua)(hua)(hua)學(xue)(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)組(zu)成(cheng)(cheng)(cheng)(cheng)(cheng),這(zhe)(zhe)些(xie)化(hua)(hua)(hua)學(xue)(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)包(bao)括白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、α1、Chemicalbookα2、β、γ-球(qiu)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai),甘(gan)油(you)三酯,總(zong)(zong)膽(dan)固(gu)醇,谷丙轉(zhuan)氨(an)酶(mei)等(deng)(deng)(deng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)含(han)(han)(han)(han)(han)有(you)(you)(you)(you)(you)(you)(you)各(ge)種(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、多(duo)(duo)肽(tai)(tai)(tai)(tai)(tai)、脂肪、碳水(shui)化(hua)(hua)(hua)合(he)(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)(yin)子(zi)(zi)(zi)(zi)、激(ji)(ji)(ji)素(su)(su)(su)(su)、無(wu)(wu)(wu)機(ji)(ji)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)等(deng)(deng)(deng)，這(zhe)(zhe)些(xie)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)對(dui)(dui)(dui)(dui)促(cu)(cu)進(jin)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)或(huo)(huo)抑制(zhi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)活性(xing)(xing)是(shi)(shi)(shi)達到(dao)(dao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)平(ping)衡(heng)的(de)(de)(de)(de)(de)(de)(de)(de)。儲存條(tiao)件(jian) 需要(yao)(yao)長(chang)(chang)(chang)期(qi)保(bao)存的(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必須(xu)(xu)儲存于(yu)-20℃-70℃低(di)(di)溫冰箱中(zhong)(zhong)(zhong)(zhong).4℃冰箱中(zhong)(zhong)(zhong)(zhong)保(bao)存時(shi)間(jian)切勿(wu)超(chao)過(guo)(guo)(guo)(guo)1個月.由(you)于(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結(jie)(jie)(jie)冰時(shi)體(ti)(ti)積(ji)會(hui)增(zeng)(zeng)加約10%,因(yin)(yin)(yin)(yin)此(ci),血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)凍入低(di)(di)溫冰箱前,必須(xu)(xu)預留一(yi)(yi)(yi)(yi)定(ding)體(ti)(ti)積(ji)空間(jian),否則易(yi)發生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)污染(ran)或(huo)(huo)玻璃瓶(ping)凍裂.應(ying)用(yong)(yong)(yong)(yong)(yong)(yong) 驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)培養(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)用(yong)(yong)(yong)(yong)(yong)(yong)量(liang)(liang)(liang)很(hen)大的(de)(de)(de)(de)(de)(de)(de)(de)天(tian)然培養(yang)(yang)(yang)基(ji)(ji)(ji)，含(han)(han)(han)(han)(han)有(you)(you)(you)(you)(you)(you)(you)豐富(fu)的(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)必須(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)養(yang)(yang)(yang)成(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)，常(chang)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)體(ti)(ti)外(wai)(wai)培養(yang)(yang)(yang)，具(ju)有(you)(you)(you)(you)(you)(you)(you)極為(wei)重(zhong)要(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)功能。提(ti)(ti)(ti)供(gong)(gong)(gong)對(dui)(dui)(dui)(dui)維(wei)(wei)(wei)(wei)(wei)(wei)持(chi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)指(zhi)數(shu)(shu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)的(de)(de)(de)(de)(de)(de)(de)(de)激(ji)(ji)(ji)素(su)(su)(su)(su)，基(ji)(ji)(ji)礎培養(yang)(yang)(yang)基(ji)(ji)(ji)中(zhong)(zhong)(zhong)(zhong)沒有(you)(you)(you)(you)(you)(you)(you)或(huo)(huo)量(liang)(liang)(liang)很(hen)少的(de)(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)養(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)，以及(ji)(ji)主(zhu)要(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)低(di)(di)分(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)(zi)(zi)營(ying)(ying)養(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)。提(ti)(ti)(ti)供(gong)(gong)(gong)結(jie)(jie)(jie)合(he)(he)(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)，能識(shi)別維(wei)(wei)(wei)(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素(su)(su)(su)(su)、脂類(lei)、金屬(shu)和(he)其(qi)他(ta)激(ji)(ji)(ji)素(su)(su)(su)(su)等(deng)(deng)(deng)，能結(jie)(jie)(jie)合(he)(he)(he)或(huo)(huo)調變(bian)它們所(suo)結(jie)(jie)(jie)合(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)活力。有(you)(you)(you)(you)(you)(you)(you)些(xie)情況下結(jie)(jie)(jie)合(he)(he)(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)能與(yu)有(you)(you)(you)(you)(you)(you)(you)毒(du)金屬(shu)和(he)熱原(yuan)(yuan)(yuan)(yuan)質(zhi)(zhi)(zhi)結(jie)(jie)(jie)合(he)(he)(he)，起(qi)(qi)到(dao)(dao)解毒(du)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。是(shi)(shi)(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼壁、鋪展在(zai)(zai)(zai)塑(su)料(liao)培養(yang)(yang)(yang)基(ji)(ji)(ji)質(zhi)(zhi)(zhi)上所(suo)需因(yin)(yin)(yin)(yin)子(zi)(zi)(zi)(zi)來(lai)源。起(qi)(qi)酸(suan)(suan)堿度(du)緩沖液(ye)(ye)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。提(ti)(ti)(ti)供(gong)(gong)(gong)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)抑制(zhi)劑，使(shi)(shi)(shi)(shi)在(zai)(zai)(zai)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)傳代(dai)時(shi)使(shi)(shi)(shi)(shi)剩余胰蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)失活，保(bao)護(hu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)不(bu)(bu)(bu)(bu)(bu)(bu)受(shou)傷害(hai)。驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)（去(qu)(qu)紅細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、無(wu)(wu)(wu)菌(jun)(jun)過(guo)(guo)(guo)(guo)濾(lv)）主(zhu)要(yao)(yao)成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)由(you)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)去(qu)(qu)除(chu)(chu)纖(xian)(xian)(xian)維(wei)(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)(yuan)而(er)(er)(er)形成(cheng)(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)種(zhong)(zhong)很(hen)復(fu)(fu)(fu)(fu)雜的(de)(de)(de)(de)(de)(de)(de)(de)混(hun)合(he)(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)，其(qi)組(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)雖(sui)大部(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)已知，但還(huan)(huan)有(you)(you)(you)(you)(you)(you)(you)一(yi)(yi)(yi)(yi)部(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)尚(shang)不(bu)(bu)(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，且(qie)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)及(ji)(ji)含(han)(han)(han)(han)(han)量(liang)(liang)(liang)常(chang)隨供(gong)(gong)(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)別、年(nian)齡(ling)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)條(tiao)件(jian)和(he)營(ying)(ying)養(yang)(yang)(yang)條(tiao)件(jian)不(bu)(bu)(bu)(bu)(bu)(bu)同而(er)(er)(er)異。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)中(zhong)(zhong)(zhong)(zhong)不(bu)(bu)(bu)(bu)(bu)(bu)含(han)(han)(han)(han)(han)纖(xian)(xian)(xian)維(wei)(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)(yuan)的(de)(de)(de)(de)(de)(de)(de)(de)膠狀(zhuang)液(ye)(ye)體(ti)(ti),具(ju)有(you)(you)(you)(you)(you)(you)(you)維(wei)(wei)(wei)(wei)(wei)(wei)持(chi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)正(zheng)(zheng)常(chang)粘(zhan)度(du)、酸(suan)(suan)堿度(du)、滲透(tou)(tou)壓(ya)等(deng)(deng)(deng)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。它主(zhu)要(yao)(yao)由(you)水(shui)和(he)各(ge)種(zhong)(zhong)化(hua)(hua)(hua)學(xue)(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)組(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)，這(zhe)(zhe)些(xie)化(hua)(hua)(hua)學(xue)(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)包(bao)括白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)，α1、α2、β、γ-球(qiu)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)，甘(gan)油(you)三酯，總(zong)(zong)膽(dan)固(gu)醇，谷丙轉(zhuan)氨(an)酶(mei)等(deng)(deng)(deng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)含(han)(han)(han)(han)(han)有(you)(you)(you)(you)(you)(you)(you)各(ge)種(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、多(duo)(duo)肽(tai)(tai)(tai)(tai)(tai)、脂肪、碳水(shui)化(hua)(hua)(hua)合(he)(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)(yin)子(zi)(zi)(zi)(zi)、激(ji)(ji)(ji)素(su)(su)(su)(su)、無(wu)(wu)(wu)機(ji)(ji)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)等(deng)(deng)(deng)，這(zhe)(zhe)些(xie)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)對(dui)(dui)(dui)(dui)促(cu)(cu)進(jin)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)或(huo)(huo)抑制(zhi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)活性(xing)(xing)是(shi)(shi)(shi)達到(dao)(dao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)平(ping)衡(heng)的(de)(de)(de)(de)(de)(de)(de)(de)。對(dui)(dui)(dui)(dui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)和(he)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)研(yan)(yan)究(jiu)雖(sui)有(you)(you)(you)(you)(you)(you)(you)很(hen)大進(jin)展，但仍存在(zai)(zai)(zai)一(yi)(yi)(yi)(yi)些(xie)問(wen)題(ti)。主(zhu)要(yao)(yao)是(shi)(shi)(shi)：第(di)一(yi)(yi)(yi)(yi)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)可(ke)(ke)(ke)能有(you)(you)(you)(you)(you)(you)(you)幾百種(zhong)(zhong)之(zhi)多(duo)(duo)，對(dui)(dui)(dui)(dui)其(qi)準確的(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)、含(han)(han)(han)(han)(han)量(liang)(liang)(liang)及(ji)(ji)其(qi)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)機(ji)(ji)制(zhi)仍不(bu)(bu)(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，尤(you)其(qi)是(shi)(shi)(shi)對(dui)(dui)(dui)(dui)其(qi)中(zhong)(zhong)(zhong)(zhong)一(yi)(yi)(yi)(yi)些(xie)多(duo)(duo)肽(tai)(tai)(tai)(tai)(tai)類(lei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)(yin)子(zi)(zi)(zi)(zi)、激(ji)(ji)(ji)素(su)(su)(su)(su)和(he)脂類(lei)等(deng)(deng)(deng)尚(shang)未充分(fen)(fen)(fen)(fen)(fen)(fen)認識(shi)，這(zhe)(zhe)給研(yan)(yan)究(jiu)工(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)帶(dai)來(lai)許多(duo)(duo)困(kun)難；第(di)二：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都是(shi)(shi)(shi)批量(liang)(liang)(liang)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產，各(ge)批量(liang)(liang)(liang)之(zhi)間(jian)差(cha)異很(hen)大，而(er)(er)(er)且(qie)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)保(bao)存期(qi)至多(duo)(duo)一(yi)(yi)(yi)(yi)年(nian)，因(yin)(yin)(yin)(yin)此(ci)，要(yao)(yao)保(bao)證(zheng)每(mei)批血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)相(xiang)(xiang)似性(xing)(xing)極為(wei)困(kun)難，從而(er)(er)(er)使(shi)(shi)(shi)(shi)實驗的(de)(de)(de)(de)(de)(de)(de)(de)標準化(hua)(hua)(hua)和(he)連續性(xing)(xing)受(shou)到(dao)(dao)限制(zhi)；第(di)三：不(bu)(bu)(bu)(bu)(bu)(bu)能排除(chu)(chu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)含(han)(han)(han)(han)(han)有(you)(you)(you)(you)(you)(you)(you)易(yi)變(bian)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)，這(zhe)(zhe)被認為(wei)是(shi)(shi)(shi)“瓶(ping)中(zhong)(zhong)(zhong)(zhong)惡化(hua)(hua)(hua)”的(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)(yuan)(yuan)(yuan)因(yin)(yin)(yin)(yin)之(zhi)一(yi)(yi)(yi)(yi)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)主(zhu)要(yao)(yao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)●提(ti)(ti)(ti)供(gong)(gong)(gong)基(ji)(ji)(ji)本(ben)(ben)營(ying)(ying)養(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)：氨(an)基(ji)(ji)(ji)酸(suan)(suan)、維(wei)(wei)(wei)(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素(su)(su)(su)(su)、無(wu)(wu)(wu)機(ji)(ji)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、脂類(lei)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)、核(he)酸(suan)(suan)衍生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)等(deng)(deng)(deng)，是(shi)(shi)(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)必須(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)。●提(ti)(ti)(ti)供(gong)(gong)(gong)激(ji)(ji)(ji)素(su)(su)(su)(su)和(he)各(ge)種(zhong)(zhong)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)(yin)子(zi)(zi)(zi)(zi)：胰島素(su)(su)(su)(su)、腎上腺皮質(zhi)(zhi)(zhi)激(ji)(ji)(ji)素(su)(su)(su)(su)（氫(qing)化(hua)(hua)(hua)可(ke)(ke)(ke)的(de)(de)(de)(de)(de)(de)(de)(de)松、地(di)塞米松）、類(lei)固(gu)醇激(ji)(ji)(ji)素(su)(su)(su)(su)（雌二醇、睪酮(tong)、孕(yun)酮(tong)）等(deng)(deng)(deng)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)(yin)子(zi)(zi)(zi)(zi)如成(cheng)(cheng)(cheng)(cheng)(cheng)纖(xian)(xian)(xian)維(wei)(wei)(wei)(wei)(wei)(wei)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)(yin)子(zi)(zi)(zi)(zi)、表(biao)皮生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)(yin)子(zi)(zi)(zi)(zi)、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)板生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)(yin)子(zi)(zi)(zi)(zi)等(deng)(deng)(deng)。●提(ti)(ti)(ti)供(gong)(gong)(gong)結(jie)(jie)(jie)合(he)(he)(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)：結(jie)(jie)(jie)合(he)(he)(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)攜帶(dai)重(zhong)要(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)低(di)(di)分(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)(zi)(zi)量(liang)(liang)(liang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)，如白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜帶(dai)維(wei)(wei)(wei)(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素(su)(su)(su)(su)、脂肪、以及(ji)(ji)激(ji)(ji)(ji)素(su)(su)(su)(su)等(deng)(deng)(deng)，轉(zhuan)鐵(tie)(tie)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜帶(dai)鐵(tie)(tie)。結(jie)(jie)(jie)合(he)(he)(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)在(zai)(zai)(zai)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)代(dai)謝過(guo)(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)起(qi)(qi)重(zhong)要(yao)(yao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。●提(ti)(ti)(ti)供(gong)(gong)(gong)促(cu)(cu)接觸和(he)伸展因(yin)(yin)(yin)(yin)子(zi)(zi)(zi)(zi)使(shi)(shi)(shi)(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼壁免受(shou)機(ji)(ji)械損傷。●對(dui)(dui)(dui)(dui)培養(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)起(qi)(qi)到(dao)(dao)某些(xie)保(bao)護(hu)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)：有(you)(you)(you)(you)(you)(you)(you)一(yi)(yi)(yi)(yi)些(xie)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)，如內皮細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、骨(gu)(gu)髓(sui)(sui)樣(yang)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)可(ke)(ke)(ke)以釋放(fang)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)含(han)(han)(han)(han)(han)有(you)(you)(you)(you)(you)(you)(you)抗蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)應(ying)用(yong)(yong)(yong)(yong)(yong)(yong)驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)研(yan)(yan)究(jiu)驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)的(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)取及(ji)(ji)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)活性(xing)(xing)研(yan)(yan)究(jiu)： 在(zai)(zai)(zai)源于(yu)驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)的(de)(de)(de)(de)(de)(de)(de)(de)肽(tai)(tai)(tai)(tai)(tai)HP-6對(dui)(dui)(dui)(dui)造血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)系統(tong)的(de)(de)(de)(de)(de)(de)(de)(de)體(ti)(ti)內外(wai)(wai)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)研(yan)(yan)究(jiu)中(zhong)(zhong)(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)離(li)培養(yang)(yang)(yang)人(ren)骨(gu)(gu)髓(sui)(sui)有(you)(you)(you)(you)(you)(you)(you)核(he)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(human bone marrow nucleated cells,hBMNCs)及(ji)(ji)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)骨(gu)(gu)髓(sui)(sui)基(ji)(ji)(ji)質(zhi)(zhi)(zhi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(murinebone marrow stromal cells,mBMSCs),通過(guo)(guo)(guo)(guo)與(yu)不(bu)(bu)(bu)(bu)(bu)(bu)同濃度(du)肽(tai)(tai)(tai)(tai)(tai)HP-6(0.000 15,0.001 5,0.015,0.15,1.5μmol.L-1)共培養(yang)(yang)(yang)一(yi)(yi)(yi)(yi)定(ding)時(shi)間(jian)考察(cha)肽(tai)(tai)(tai)(tai)(tai)HP-6對(dui)(dui)(dui)(dui)骨(gu)(gu)髓(sui)(sui)造血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)相(xiang)(xiang)關(guan)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)促(cu)(cu)增(zeng)(zeng)殖(zhi)能力,并(bing)通過(guo)(guo)(guo)(guo)電鏡(jing)觀察(cha)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)后的(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)形態(tai);建立失血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)性(xing)(xing)貧(pin)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)與(yu)環(huan)磷酰(xian)胺致(zhi)貧(pin)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)2種(zhong)(zhong)貧(pin)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模型,隨機(ji)(ji)分(fen)(fen)(fen)(fen)(fen)(fen)組(zu)并(bing)灌胃不(bu)(bu)(bu)(bu)(bu)(bu)同劑量(liang)(liang)(liang)(1,0.1,0.01 mg.kg-1)肽(tai)(tai)(tai)(tai)(tai)HP-6,同時(shi)設立相(xiang)(xiang)應(ying)的(de)(de)(de)(de)(de)(de)(de)(de)對(dui)(dui)(dui)(dui)照(zhao)組(zu)灌胃等(deng)(deng)(deng)體(ti)(ti)積(ji)PBS,7 d后檢測貧(pin)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)外(wai)(wai)周(zhou)(zhou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)象(xiang),對(dui)(dui)(dui)(dui)環(huan)磷酰(xian)胺致(zhi)貧(pin)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)加測骨(gu)(gu)髓(sui)(sui)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bone marrow cells,BMCs)數(shu)(shu)。結(jie)(jie)(jie)果(guo): 肽(tai)(tai)(tai)(tai)(tai)HP-6可(ke)(ke)(ke)刺(ci)激(ji)(ji)(ji)人(ren)骨(gu)(gu)髓(sui)(sui)有(you)(you)(you)(you)(you)(you)(you)核(he)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)和(he)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)骨(gu)(gu)髓(sui)(sui)基(ji)(ji)(ji)質(zhi)(zhi)(zhi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)增(zeng)(zeng)殖(zhi)且(qie)具(ju)有(you)(you)(you)(you)(you)(you)(you)濃度(du)相(xiang)(xiang)關(guan)性(xing)(xing),0.15μmol.L-1肽(tai)(tai)(tai)(tai)(tai)HP-6使(shi)(shi)(shi)(shi)2種(zhong)(zhong)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)均達到(dao)(dao)增(zeng)(zeng)殖(zhi)率(lv)(lv),分(fen)(fen)(fen)(fen)(fen)(fen)別為(wei)152.11%,63.52%,之(zhi)后呈降低(di)(di)趨勢;電鏡(jing)結(jie)(jie)(jie)果(guo)顯示與(yu)肽(tai)(tai)(tai)(tai)(tai)HP-6作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)后的(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)微觀形態(tai)結(jie)(jie)(jie)構正(zheng)(zheng)常(chang);灌胃1 mg.kg-1肽(tai)(tai)(tai)(tai)(tai)HP-6對(dui)(dui)(dui)(dui)提(ti)(ti)(ti)高外(wai)(wai)周(zhou)(zhou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)板數(shu)(shu)量(liang)(liang)(liang)及(ji)(ji)保(bao)護(hu)環(huan)磷酰(xian)胺損傷的(de)(de)(de)(de)(de)(de)(de)(de)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)骨(gu)(gu)髓(sui)(sui)具(ju)有(you)(you)(you)(you)(you)(you)(you)一(yi)(yi)(yi)(yi)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)功效;灌胃0.1 mg.kg-1肽(tai)(tai)(tai)(tai)(tai)HP-6對(dui)(dui)(dui)(dui)提(ti)(ti)(ti)高外(wai)(wai)周(zhou)(zhou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)及(ji)(ji)紅細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)數(shu)(shu)量(liang)(liang)(liang)有(you)(you)(you)(you)(you)(you)(you)較好的(de)(de)(de)(de)(de)(de)(de)(de)效果(guo),還(huan)(huan)可(ke)(ke)(ke)對(dui)(dui)(dui)(dui)化(hua)(hua)(hua)療(liao)藥物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)后小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)的(de)(de)(de)(de)(de)(de)(de)(de)外(wai)(wai)周(zhou)(zhou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)起(qi)(qi)到(dao)(dao)一(yi)(yi)(yi)(yi)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)保(bao)護(hu)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong);灌胃0.01 mg.kg-1肽(tai)(tai)(tai)(tai)(tai)HP-6對(dui)(dui)(dui)(dui)提(ti)(ti)(ti)高外(wai)(wai)周(zhou)(zhou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)板數(shu)(shu)量(liang)(liang)(liang)具(ju)有(you)(you)(you)(you)(you)(you)(you)＊＊的(de)(de)(de)(de)(de)(de)(de)(de)效果(guo),相(xiang)(xiang)對(dui)(dui)(dui)(dui)增(zeng)(zeng)殖(zhi)率(lv)(lv)可(ke)(ke)(ke)達77.65%。結(jie)(jie)(jie)論:肽(tai)(tai)(tai)(tai)(tai)HP-6可(ke)(ke)(ke)刺(ci)激(ji)(ji)(ji)造血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)系統(tong)相(xiang)(xiang)關(guan)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)增(zeng)(zeng)殖(zhi),對(dui)(dui)(dui)(dui)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)造血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)功能與(yu)抵抗化(hua)(hua)(hua)療(liao)藥物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)損傷有(you)(you)(you)(you)(you)(you)(you)一(yi)(yi)(yi)(yi)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)增(zeng)(zeng)強作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。
豬(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿（去紅(hong)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)、無(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)過(guo)(guo)(guo)濾(lv)(lv)）規格:500ml/瓶(ping)保(bao)(bao)質(zhi)(zhi)(zhi)(zhi)(zhi)期: 五年(nian)(nian)如(ru)(ru)(ru)何(he)解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)才(cai)不(bu)會(hui)使(shi)(shi)產(chan)(chan)(chan)品質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)受(shou)損(sun)? 將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)冷凍(dong)(dong)(dong)(dong)(dong)(dong)箱(xiang)(xiang)(xiang)(xiang)取出(chu)(chu)(chu)(chu)后,先置于(yu)(yu)2-8℃冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)使(shi)(shi)之溶(rong)(rong)解(jie)(jie)(jie),然(ran)后在(zai)(zai)(zai)室溫(wen)(wen)(wen)(wen)下(xia)(xia)使(shi)(shi)之全(quan)溶(rong)(rong)。但(dan)必(bi)(bi)(bi)(bi)(bi)(bi)須(xu)(xu)(xu)(xu)注(zhu)(zhu)意(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi),溶(rong)(rong)解(jie)(jie)(jie)過(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)必(bi)(bi)(bi)(bi)(bi)(bi)須(xu)(xu)(xu)(xu)規則地(di)搖(yao)晃(huang)(huang)均(jun)(jun)勻(yun)。1.實驗(yan)(yan)(yan)(yan)(yan)時(shi)(shi)(shi)根據試(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情(qing)況(kuang)和(he)(he)性(xing)(xing)(xing)(xing)質(zhi)(zhi)(zhi)(zhi)(zhi)進(jin)行(xing)必(bi)(bi)(bi)(bi)(bi)(bi)要(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)防(fang)(fang)(fang)護(hu)。根據試(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)可(ke)(ke)(ke)能發(fa)(fa)生(sheng)(sheng)(sheng)(sheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)危險(xian)(xian)事(shi)故佩(pei)戴必(bi)(bi)(bi)(bi)(bi)(bi)要(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)防(fang)(fang)(fang)護(hu)工具,例(li)如(ru)(ru)(ru)穿好試(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)服(fu),戴橡膠手(shou)套,防(fang)(fang)(fang)護(hu)面具,防(fang)(fang)(fang)毒面具等(deng)(deng)(deng)。實驗(yan)(yan)(yan)(yan)(yan)前(qian),要(yao)(yao)(yao)(yao)(yao)注(zhu)(zhu)意(yi)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)理(li)(li)試(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)場周圍的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)安全(quan)隱患。檢(jian)查試(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)裝(zhuang)置、藥品和(he)(he)相(xiang)關物(wu)(wu)(wu)品是(shi)(shi)(shi)否有(you)(you)(you)(you)(you)(you)不(bu)符合(he)(he)(he)要(yao)(yao)(yao)(yao)(yao)求的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情(qing)況(kuang)等(deng)(deng)(deng)。 2.遵循化(hua)(hua)(hua)學(xue)藥品的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)(xing)(xing)質(zhi)(zhi)(zhi)(zhi)(zhi)和(he)(he)化(hua)(hua)(hua)學(xue)反(fan)(fan)(fan)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)規律,不(bu)盲目蠻干(gan)和(he)(he)主觀臆測(ce)化(hua)(hua)(hua)學(xue)反(fan)(fan)(fan)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)程(cheng)(cheng)。應(ying)(ying)根據化(hua)(hua)(hua)學(xue)反(fan)(fan)(fan)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)(xing)(xing)質(zhi)(zhi)(zhi)(zhi)(zhi)和(he)(he)過(guo)(guo)(guo)程(cheng)(cheng)選(xuan)擇匹(pi)配的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)反(fan)(fan)(fan)應(ying)(ying)裝(zhuang)置,不(bu)可(ke)(ke)(ke)圖省事(shi)省去必(bi)(bi)(bi)(bi)(bi)(bi)要(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)安全(quan)措施。 實驗(yan)(yan)(yan)(yan)(yan)事(shi)故雖不(bu)可(ke)(ke)(ke)預(yu)測(ce),但(dan)其(qi)(qi)危險(xian)(xian)性(xing)(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大(da)小(xiao)是(shi)(shi)(shi)可(ke)(ke)(ke)以估(gu)計到的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。保(bao)(bao)存(cun)(cun)方法(fa):(1)長(chang)期保(bao)(bao)存(cun)(cun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必(bi)(bi)(bi)(bi)(bi)(bi)須(xu)(xu)(xu)(xu)儲存(cun)(cun)于(yu)(yu)-20℃ - 70℃ 低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。4℃冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)存(cun)(cun)時(shi)(shi)(shi)間(jian)切(qie)(qie)(qie)勿(wu)(wu)超(chao)過(guo)(guo)(guo)1個月(yue)。由(you)于(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結(jie)(jie)冰(bing)(bing)時(shi)(shi)(shi)體(ti)(ti)積(ji)(ji)會(hui)增(zeng)加(jia)(jia)約10%,因(yin)(yin)(yin)此(ci),血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)凍(dong)(dong)(dong)(dong)(dong)(dong)入(ru)(ru)(ru)低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)前(qian),必(bi)(bi)(bi)(bi)(bi)(bi)須(xu)(xu)(xu)(xu)預(yu)留(liu)一(yi)(yi)定(ding)體(ti)(ti)積(ji)(ji)空(kong)(kong)間(jian),否則易(yi)發(fa)(fa)生(sheng)(sheng)(sheng)(sheng)污染或(huo)(huo)(huo)玻(bo)璃瓶(ping)凍(dong)(dong)(dong)(dong)(dong)(dong)裂(lie)(lie)。 (2)一(yi)(yi)般 提供(gong)(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)無(wu)(wu)菌(jun)(jun)(jun)(jun)(jun),無(wu)(wu)需(xu)(xu)再(zai)(zai)過(guo)(guo)(guo)濾(lv)(lv)除(chu)(chu)菌(jun)(jun)(jun)(jun)(jun)。如(ru)(ru)(ru)發(fa)(fa)現(xian)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)有(you)(you)(you)(you)(you)(you)懸(xuan)(xuan)浮(fu)(fu)物(wu)(wu)(wu),則可(ke)(ke)(ke)將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)加(jia)(jia)入(ru)(ru)(ru)培(pei)(pei)養(yang)液(ye)(ye)內(nei)一(yi)(yi)起(qi)過(guo)(guo)(guo)濾(lv)(lv),切(qie)(qie)(qie)勿(wu)(wu)直(zhi)(zhi)接(jie)過(guo)(guo)(guo)濾(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。(3)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong): 瓶(ping)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)需(xu)(xu)采(cai)用(yong)(yong)(yong)(yong)(yong)逐(zhu)(zhu)步解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)法(fa):-20℃ 至(zhi) -70℃ 低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)放(fang)(fang)入(ru)(ru)(ru)4℃冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)溶(rong)(rong)解(jie)(jie)(jie)1天(tian)。然(ran)后移入(ru)(ru)(ru)室溫(wen)(wen)(wen)(wen),待(dai)全(quan)部(bu)溶(rong)(rong)解(jie)(jie)(jie)后再(zai)(zai)分(fen)(fen)(fen)(fen)(fen)裝(zhuang)。在(zai)(zai)(zai)溶(rong)(rong)解(jie)(jie)(jie)過(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)需(xu)(xu)不(bu)斷(duan)輕(qing)輕(qing)搖(yao)晃(huang)(huang)均(jun)(jun)勻(yun)(小(xiao)心勿(wu)(wu)造(zao)成(cheng)(cheng)氣泡),使(shi)(shi)溫(wen)(wen)(wen)(wen)度(du)(du)與(yu)成(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)均(jun)(jun)一(yi)(yi),減(jian)少沉(chen)淀(dian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)(fa)生(sheng)(sheng)(sheng)(sheng)。切(qie)(qie)(qie)勿(wu)(wu)直(zhi)(zhi)接(jie)將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)-20℃進(jin)入(ru)(ru)(ru)37℃解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong),這(zhe)(zhe)樣(yang)因(yin)(yin)(yin)溫(wen)(wen)(wen)(wen)度(du)(du)改變(bian)太(tai)大(da),容易(yi)造(zao)成(cheng)(cheng)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)凝(ning)集而(er)(er)出(chu)(chu)(chu)(chu)現(xian)沉(chen)淀(dian)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)留(liu)應(ying)(ying)當(dang)留(liu)意(yi)以下(xia)(xia)幾點:1需(xu)(xu)求長(chang)時(shi)(shi)(shi)間(jian)保(bao)(bao)存(cun)(cun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)有(you)(you)(you)(you)(you)(you)必(bi)(bi)(bi)(bi)(bi)(bi)要(yao)(yao)(yao)(yao)(yao)儲存(cun)(cun)于(yu)(yu)-20℃ - 70℃ 低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong).4℃冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)存(cun)(cun)時(shi)(shi)(shi)間(jian)切(qie)(qie)(qie)勿(wu)(wu)超(chao)越(yue)1個月(yue).因(yin)(yin)(yin)為(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結(jie)(jie)冰(bing)(bing)時(shi)(shi)(shi)體(ti)(ti)積(ji)(ji)會(hui)添(tian)加(jia)(jia)約10%,因(yin)(yin)(yin)此(ci),血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)凍(dong)(dong)(dong)(dong)(dong)(dong)入(ru)(ru)(ru)低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)前(qian),有(you)(you)(you)(you)(you)(you)必(bi)(bi)(bi)(bi)(bi)(bi)要(yao)(yao)(yao)(yao)(yao)預(yu)留(liu)必(bi)(bi)(bi)(bi)(bi)(bi)定(ding)體(ti)(ti)積(ji)(ji)空(kong)(kong)間(jian),否則易(yi)發(fa)(fa)生(sheng)(sheng)(sheng)(sheng)污染或(huo)(huo)(huo)玻(bo)璃瓶(ping)凍(dong)(dong)(dong)(dong)(dong)(dong)裂(lie)(lie).2.熱(re)(re)(re)滅(mie)活(huo)(huo)是(shi)(shi)(shi)指(zhi)(zhi)56℃, 30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)加(jia)(jia)熱(re)(re)(re)已(yi)(yi)完(wan)全(quan)凍(dong)(dong)(dong)(dong)(dong)(dong)住(zhu)(zhu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).加(jia)(jia)熱(re)(re)(re)過(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)須(xu)(xu)(xu)(xu)規矩搖(yao)晃(huang)(huang)均(jun)(jun)勻(yun).此(ci)熱(re)(re)(re)處理(li)(li)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)目的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)使(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補體(ti)(ti)成(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(complement)滅(mie)活(huo)(huo).除(chu)(chu)非(fei)(fei)有(you)(you)(you)(you)(you)(you)必(bi)(bi)(bi)(bi)(bi)(bi)要(yao)(yao)(yao)(yao)(yao),一(yi)(yi)般不(bu)建議(yi)作(zuo)(zuo)(zuo)(zuo)(zuo)此(ci)熱(re)(re)(re)處理(li)(li),因(yin)(yin)(yin)為(wei)熱(re)(re)(re)處理(li)(li)會(hui)構(gou)成(cheng)(cheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)堆積(ji)(ji)物(wu)(wu)(wu)明(ming)顯(xian)增(zeng)多,而(er)(er)且還會(hui)影(ying)(ying)響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang).補體(ti)(ti)參與(yu)反(fan)(fan)(fan)響(xiang)有(you)(you)(you)(you)(you)(you):細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)毒作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong), 滑(hua)潤肌細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)縮短(duan), 肥(fei)大(da)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)和(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)板開(kai)釋組(zu)胺, 增(zeng)強吞噬(shi)作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong), 推(tui)(tui)動和(he)(he)發(fa)(fa)生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)學(xue)趨化(hua)(hua)(hua)和(he)(he)活(huo)(huo)化(hua)(hua)(hua).3.瓶(ping)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)凍(dong)(dong)(dong)(dong)(dong)(dong)住(zhu)(zhu)需(xu)(xu)選(xuan)用(yong)(yong)(yong)(yong)(yong)逐(zhu)(zhu)步凍(dong)(dong)(dong)(dong)(dong)(dong)住(zhu)(zhu)法(fa):-20℃ 至(zhi) -70℃ 低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)放(fang)(fang)入(ru)(ru)(ru)4℃冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)溶(rong)(rong)解(jie)(jie)(jie)1天(tian).然(ran)后移入(ru)(ru)(ru)室溫(wen)(wen)(wen)(wen),待(dai)全(quan)部(bu)溶(rong)(rong)解(jie)(jie)(jie)后再(zai)(zai)分(fen)(fen)(fen)(fen)(fen)裝(zhuang).在(zai)(zai)(zai)溶(rong)(rong)解(jie)(jie)(jie)過(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)需(xu)(xu)不(bu)斷(duan)悄(qiao)悄(qiao)搖(yao)晃(huang)(huang)均(jun)(jun)勻(yun)(當(dang)心勿(wu)(wu)構(gou)成(cheng)(cheng)氣泡),使(shi)(shi)溫(wen)(wen)(wen)(wen)度(du)(du)與(yu)成(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)均(jun)(jun)一(yi)(yi),削減(jian)堆積(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)(fa)生(sheng)(sheng)(sheng)(sheng).切(qie)(qie)(qie)勿(wu)(wu)直(zhi)(zhi)接(jie)將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)-20℃進(jin)入(ru)(ru)(ru)37℃凍(dong)(dong)(dong)(dong)(dong)(dong)住(zhu)(zhu),這(zhe)(zhe)么(me)因(yin)(yin)(yin)溫(wen)(wen)(wen)(wen)度(du)(du)改動太(tai)大(da),簡略(lve)構(gou)成(cheng)(cheng)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)凝(ning)集而(er)(er)出(chu)(chu)(chu)(chu)現(xian)堆積(ji)(ji).4.一(yi)(yi)般 供(gong)(gong)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)無(wu)(wu)菌(jun)(jun)(jun)(jun)(jun),無(wu)(wu)需(xu)(xu)再(zai)(zai)過(guo)(guo)(guo)濾(lv)(lv)除(chu)(chu)菌(jun)(jun)(jun)(jun)(jun).如(ru)(ru)(ru)發(fa)(fa)現(xian)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)有(you)(you)(you)(you)(you)(you)懸(xuan)(xuan)浮(fu)(fu)物(wu)(wu)(wu),則可(ke)(ke)(ke)將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)參加(jia)(jia)培(pei)(pei)養(yang)液(ye)(ye)內(nei)一(yi)(yi)同過(guo)(guo)(guo)濾(lv)(lv),切(qie)(qie)(qie)勿(wu)(wu)直(zhi)(zhi)接(jie)過(guo)(guo)(guo)濾(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).5.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)堆積(ji)(ji)物(wu)(wu)(wu) 絮(xu)狀(zhuang)物(wu)(wu)(wu):主要(yao)(yao)(yao)(yao)(yao)是(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)變(bian)性(xing)(xing)(xing)(xing)及凍(dong)(dong)(dong)(dong)(dong)(dong)住(zhu)(zhu)后血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)纖(xian)(xian)(xian)維(wei)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)構(gou)成(cheng)(cheng),這(zhe)(zhe)些絮(xu)狀(zhuang)物(wu)(wu)(wu)不(bu)會(hui)影(ying)(ying)響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本身(shen)(shen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang).可(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)離心3000rpm, 5分(fen)(fen)(fen)(fen)(fen)鐘(zhong)去掉,也(ye)(ye)可(ke)(ke)(ke)不(bu)用(yong)(yong)(yong)(yong)(yong)處理(li)(li). 顯(xian)微(wei)鏡(jing)下(xia)(xia)"小(xiao)黑(hei)點":通(tong)過(guo)(guo)(guo)熱(re)(re)(re)處理(li)(li)過(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),堆積(ji)(ji)物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)構(gou)成(cheng)(cheng)會(hui)明(ming)顯(xian)增(zeng)多.有(you)(you)(you)(you)(you)(you)些堆積(ji)(ji)物(wu)(wu)(wu)在(zai)(zai)(zai)顯(xian)微(wei)鏡(jing)下(xia)(xia)查詢(xun)象"小(xiao)黑(hei)點",常(chang)(chang)(chang)誤認為(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)受(shou)污染.一(yi)(yi)般情(qing)況(kuang)下(xia)(xia),此(ci)小(xiao)黑(hei)點不(bu)會(hui)影(ying)(ying)響(xiang)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長(chang),但(dan)假(jia)(jia)如(ru)(ru)(ru)懷疑血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang),則應(ying)(ying)當(dang)即停止使(shi)(shi)用(yong)(yong)(yong)(yong)(yong),替換另一(yi)(yi)批號(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).6.切(qie)(qie)(qie)勿(wu)(wu)將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)37℃放(fang)(fang)置太(tai)久,否則血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)會(hui)變(bian)得(de)渾濁,一(yi)(yi)同血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)(you)(you)(you)(you)效成(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)會(hui)破會(hui)而(er)(er)影(ying)(ying)響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)背景豬(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)系采(cai)用(yong)(yong)(yong)(yong)(yong)成(cheng)(cheng)年(nian)(nian)健康(kang)豬(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)為(wei)原(yuan)料加(jia)(jia)工而(er)(er)成(cheng)(cheng)，經(jing)(jing)無(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)采(cai)集、分(fen)(fen)(fen)(fen)(fen)離、微(wei)孔過(guo)(guo)(guo)濾(lv)(lv)分(fen)(fen)(fen)(fen)(fen)裝(zhuang)而(er)(er)成(cheng)(cheng)。無(wu)(wu)支(zhi)原(yuan)體(ti)(ti)、無(wu)(wu)噬(shi)菌(jun)(jun)(jun)(jun)(jun)體(ti)(ti)，內(nei)毒素(su)(su)含量(liang)(liang)(liang)(liang)低(di)(di)于(yu)(yu)50EU/ml。豬(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（無(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)過(guo)(guo)(guo)濾(lv)(lv)）在(zai)(zai)(zai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)培(pei)(pei)養(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)：1.提供(gong)(gong)基本營養(yang)物(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)：氨基酸、維(wei)生(sheng)(sheng)(sheng)(sheng)素(su)(su)、無(wu)(wu)機物(wu)(wu)(wu)、脂類(lei)物(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)、核(he)酸衍生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)等(deng)(deng)(deng)，是(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長(chang)必(bi)(bi)(bi)(bi)(bi)(bi)須(xu)(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)。2.提供(gong)(gong)激(ji)(ji)素(su)(su)和(he)(he)各種生(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)：胰(yi)島素(su)(su)、腎上(shang)腺皮(pi)質(zhi)(zhi)(zhi)(zhi)(zhi)激(ji)(ji)素(su)(su)（地(di)塞(sai)米松）、類(lei)固醇(chun)激(ji)(ji)素(su)(su)（雌二醇(chun)、睪酮、孕酮）等(deng)(deng)(deng)。生(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)如(ru)(ru)(ru)成(cheng)(cheng)纖(xian)(xian)(xian)維(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)、表皮(pi)生(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)板生(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)等(deng)(deng)(deng)。3.提供(gong)(gong)結(jie)(jie)合(he)(he)(he)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)：結(jie)(jie)合(he)(he)(he)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)攜(xie)帶重要(yao)(yao)(yao)(yao)(yao)地(di)低(di)(di)分(fen)(fen)(fen)(fen)(fen)子(zi)量(liang)(liang)(liang)(liang)物(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)，如(ru)(ru)(ru)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜(xie)帶維(wei)生(sheng)(sheng)(sheng)(sheng)素(su)(su)、脂肪、以及激(ji)(ji)素(su)(su)等(deng)(deng)(deng)，轉鐵蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜(xie)帶鐵。結(jie)(jie)合(he)(he)(he)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)在(zai)(zai)(zai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)代(dai)(dai)謝過(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)起(qi)重要(yao)(yao)(yao)(yao)(yao)作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。提供(gong)(gong)促接(jie)觸和(he)(he)伸(shen)展因(yin)(yin)(yin)子(zi)使(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)貼(tie)壁免受(shou)機械損(sun)傷。4.對培(pei)(pei)養(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)起(qi)到某些保(bao)(bao)護(hu)作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)：有(you)(you)(you)(you)(you)(you)一(yi)(yi)些細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)，如(ru)(ru)(ru)內(nei)皮(pi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)、骨(gu)髓(sui)樣(yang)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)可(ke)(ke)(ke)以釋放(fang)(fang)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)含有(you)(you)(you)(you)(you)(you)抗蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)成(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)，起(qi)到中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。這(zhe)(zhe)種作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)偶然(ran)發(fa)(fa)現(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，現(xian)在(zai)(zai)(zai)則有(you)(you)(you)(you)(you)(you)目的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)來終(zhong)止胰(yi)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消化(hua)(hua)(hua)作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。因(yin)(yin)(yin)為(wei)胰(yi)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)已(yi)(yi)經(jing)(jing)被廣泛用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)貼(tie)壁細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消化(hua)(hua)(hua)傳(chuan)(chuan)代(dai)(dai)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)形(xing)成(cheng)(cheng)了血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)粘(zhan)度(du)(du)，可(ke)(ke)(ke)以保(bao)(bao)護(hu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)免受(shou)機械損(sun)傷，特(te)別是(shi)(shi)(shi)在(zai)(zai)(zai)懸(xuan)(xuan)浮(fu)(fu)培(pei)(pei)養(yang)攪拌時(shi)(shi)(shi)，粘(zhan)度(du)(du)起(qi)到重要(yao)(yao)(yao)(yao)(yao)作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)還含有(you)(you)(you)(you)(you)(you)一(yi)(yi)些微(wei)量(liang)(liang)(liang)(liang)元素(su)(su)和(he)(he)離子(zi)，他(ta)們在(zai)(zai)(zai)代(dai)(dai)謝解(jie)(jie)(jie)毒中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)起(qi)重要(yao)(yao)(yao)(yao)(yao)作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)(ru)SeO3,硒等(deng)(deng)(deng)。豬(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿（去紅(hong)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)、無(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)過(guo)(guo)(guo)濾(lv)(lv)）使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)注(zhu)(zhu)意(yi)事(shi)項1.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)采(cai)用(yong)(yong)(yong)(yong)(yong)逐(zhu)(zhu)步解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)法(fa)（-20℃→2—8℃→25℃或(huo)(huo)(huo)37℃），解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)過(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)必(bi)(bi)(bi)(bi)(bi)(bi)須(xu)(xu)(xu)(xu)隨時(shi)(shi)(shi)輕(qing)搖(yao)，使(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)受(shou)熱(re)(re)(re)溫(wen)(wen)(wen)(wen)度(du)(du)均(jun)(jun)勻(yun)。2. 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)凝(ning)絮(xu)物(wu)(wu)(wu)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)后會(hui)出(chu)(chu)(chu)(chu)現(xian)少量(liang)(liang)(liang)(liang)片狀(zhuang)或(huo)(huo)(huo)絮(xu)狀(zhuang)物(wu)(wu)(wu)析(xi)出(chu)(chu)(chu)(chu)，這(zhe)(zhe)主要(yao)(yao)(yao)(yao)(yao)是(shi)(shi)(shi)由(you)于(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)內(nei)不(bu)穩定(ding)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)變(bian)性(xing)(xing)(xing)(xing)、纖(xian)(xian)(xian)維(wei)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)析(xi)出(chu)(chu)(chu)(chu)形(xing)成(cheng)(cheng)沉(chen)淀(dian)物(wu)(wu)(wu)，實驗(yan)(yan)(yan)(yan)(yan)證明(ming)沉(chen)淀(dian)物(wu)(wu)(wu)不(bu)會(hui)影(ying)(ying)響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本身(shen)(shen)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)。;3. 熱(re)(re)(re)滅(mie)活(huo)(huo)本產(chan)(chan)(chan)品除(chu)(chu)注(zhu)(zhu)明(ming)外(wai)均(jun)(jun)未滅(mie)活(huo)(huo)。如(ru)(ru)(ru)果(guo)必(bi)(bi)(bi)(bi)(bi)(bi)須(xu)(xu)(xu)(xu)滅(mie)活(huo)(huo)，請嚴格按照56℃30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)（水浴）處理(li)(li)已(yi)(yi)解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。4. 常(chang)(chang)(chang)溫(wen)(wen)(wen)(wen)狀(zhuang)態下(xia)(xia)短(duan)期融(rong)化(hua)(hua)(hua)并(bing)不(bu)會(hui)影(ying)(ying)響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)。5.產(chan)(chan)(chan)品有(you)(you)(you)(you)(you)(you)效期，檢(jian)定(ding)合(he)(he)(he)格之日(ri)起(qi)有(you)(you)(you)(you)(you)(you)效期5年(nian)(nian)。解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)：將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)冷凍(dong)(dong)(dong)(dong)(dong)(dong)箱(xiang)(xiang)(xiang)(xiang)取出(chu)(chu)(chu)(chu)后，先置于(yu)(yu)2-8℃冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)使(shi)(shi)之溶(rong)(rong)解(jie)(jie)(jie)，然(ran)后在(zai)(zai)(zai)室溫(wen)(wen)(wen)(wen)下(xia)(xia)使(shi)(shi)之全(quan)溶(rong)(rong)。但(dan)必(bi)(bi)(bi)(bi)(bi)(bi)須(xu)(xu)(xu)(xu)注(zhu)(zhu)意(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)，溶(rong)(rong)解(jie)(jie)(jie)過(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)必(bi)(bi)(bi)(bi)(bi)(bi)須(xu)(xu)(xu)(xu)規則地(di)搖(yao)晃(huang)(huang)均(jun)(jun)勻(yun)。二、保(bao)(bao)存(cun)(cun)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)應(ying)(ying)保(bao)(bao)存(cun)(cun)在(zai)(zai)(zai)-5℃至(zhi)-2O℃若(ruo)存(cun)(cun)放(fang)(fang)于(yu)(yu)4℃時(shi)(shi)(shi)，請勿(wu)(wu)超(chao)過(guo)(guo)(guo)一(yi)(yi)個月(yue)。若(ruo)一(yi)(yi)次無(wu)(wu)法(fa)用(yong)(yong)(yong)(yong)(yong)完(wan)一(yi)(yi)瓶(ping)，建議(yi)將(jiang)(jiang)(jiang)無(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)分(fen)(fen)(fen)(fen)(fen)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)至(zhi)恰當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)滅(mie)菌(jun)(jun)(jun)(jun)(jun)容器內(nei)，再(zai)(zai)放(fang)(fang)回冷凍(dong)(dong)(dong)(dong)(dong)(dong)。豬(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（無(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)過(guo)(guo)(guo)濾(lv)(lv)）注(zhu)(zhu)意(yi)事(shi)項：（1）有(you)(you)(you)(you)(you)(you)少量(liang)(liang)(liang)(liang)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)結(jie)(jie)塊析(xi)出(chu)(chu)(chu)(chu)不(bu)影(ying)(ying)響(xiang)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。未融(rong)化(hua)(hua)(hua)或(huo)(huo)(huo)融(rong)化(hua)(hua)(hua)未經(jing)(jing)搖(yao)勻(yun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)禁止直(zhi)(zhi)接(jie)放(fang)(fang)入(ru)(ru)(ru)高溫(wen)(wen)(wen)(wen)水浴內(nei)加(jia)(jia)溫(wen)(wen)(wen)(wen)。減(jian)少血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)重復凍(dong)(dong)(dong)(dong)(dong)(dong)融(rong)。若(ruo)您一(yi)(yi)次無(wu)(wu)法(fa)用(yong)(yong)(yong)(yong)(yong)完(wan)一(yi)(yi)瓶(ping)，建議(yi)您無(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)分(fen)(fen)(fen)(fen)(fen)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)至(zhi)恰當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)滅(mie)菌(jun)(jun)(jun)(jun)(jun)容器內(nei)，再(zai)(zai)放(fang)(fang)回冷凍(dong)(dong)(dong)(dong)(dong)(dong)。（2）長(chang)期保(bao)(bao)存(cun)(cun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必(bi)(bi)(bi)(bi)(bi)(bi)須(xu)(xu)(xu)(xu)儲存(cun)(cun)于(yu)(yu)-20℃-70℃低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。4℃冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)存(cun)(cun)時(shi)(shi)(shi)間(jian)切(qie)(qie)(qie)勿(wu)(wu)超(chao)過(guo)(guo)(guo)1個月(yue)。由(you)于(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結(jie)(jie)冰(bing)(bing)時(shi)(shi)(shi)體(ti)(ti)積(ji)(ji)會(hui)增(zeng)加(jia)(jia)約10%,因(yin)(yin)(yin)此(ci)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)凍(dong)(dong)(dong)(dong)(dong)(dong)入(ru)(ru)(ru)低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)前(qian)，必(bi)(bi)(bi)(bi)(bi)(bi)須(xu)(xu)(xu)(xu)預(yu)留(liu)一(yi)(yi)定(ding)體(ti)(ti)積(ji)(ji)空(kong)(kong)間(jian)，否則易(yi)發(fa)(fa)生(sheng)(sheng)(sheng)(sheng)污染或(huo)(huo)(huo)玻(bo)璃瓶(ping)凍(dong)(dong)(dong)(dong)(dong)(dong)裂(lie)(lie)。（3）一(yi)(yi)般廠(chang)商提供(gong)(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)無(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)，無(wu)(wu)需(xu)(xu)再(zai)(zai)過(guo)(guo)(guo)濾(lv)(lv)除(chu)(chu)菌(jun)(jun)(jun)(jun)(jun)。如(ru)(ru)(ru)發(fa)(fa)現(xian)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)有(you)(you)(you)(you)(you)(you)懸(xuan)(xuan)浮(fu)(fu)物(wu)(wu)(wu)，則可(ke)(ke)(ke)將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)加(jia)(jia)入(ru)(ru)(ru)培(pei)(pei)養(yang)液(ye)(ye)內(nei)一(yi)(yi)起(qi)過(guo)(guo)(guo)濾(lv)(lv)，切(qie)(qie)(qie)勿(wu)(wu)直(zhi)(zhi)接(jie)過(guo)(guo)(guo)濾(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。介紹 疫(yi)苗(miao)(miao)在(zai)(zai)(zai)預(yu)防(fang)(fang)(fang)豬(zhu)(zhu)(zhu)(zhu)常(chang)(chang)(chang)見傳(chuan)(chuan)染病(bing)方面起(qi)著非(fei)(fei)常(chang)(chang)(chang)重要(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。 它包括豬(zhu)(zhu)(zhu)(zhu)瘟(wen)疫(yi)苗(miao)(miao)、豬(zhu)(zhu)(zhu)(zhu)肺炎(yan)(yan)支(zhi)原(yuan)體(ti)(ti)疫(yi)苗(miao)(miao)以及正處在(zai)(zai)(zai)研發(fa)(fa)熱(re)(re)(re)點中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)非(fei)(fei)洲豬(zhu)(zhu)(zhu)(zhu)瘟(wen)等(deng)(deng)(deng)疫(yi)苗(miao)(miao)。 在(zai)(zai)(zai)豬(zhu)(zhu)(zhu)(zhu)病(bing)毒疫(yi)苗(miao)(miao)和(he)(he)肺炎(yan)(yan)支(zhi)原(yuan)體(ti)(ti)疫(yi)苗(miao)(miao)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)常(chang)(chang)(chang)見的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)用(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)養(yang)液(ye)(ye)成(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)。 尤其(qi)(qi)是(shi)(shi)(shi)豬(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)豬(zhu)(zhu)(zhu)(zhu)肺炎(yan)(yan)支(zhi)原(yuan)體(ti)(ti)疫(yi)苗(miao)(miao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)。在(zai)(zai)(zai)對細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)和(he)(he)活(huo)(huo)疫(yi)苗(miao)(miao)進(jin)行(xing)支(zhi)原(yuan)體(ti)(ti)檢(jian)驗(yan)(yan)(yan)(yan)(yan)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，《獸(shou)藥典(dian)》也(ye)(ye)明(ming)確推(tui)(tui)薦了豬(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)作(zuo)(zuo)(zuo)(zuo)(zuo)為(wei)支(zhi)原(yuan)體(ti)(ti)檢(jian)驗(yan)(yan)(yan)(yan)(yan)用(yong)(yong)(yong)(yong)(yong)液(ye)(ye)體(ti)(ti)培(pei)(pei)養(yang)基的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)成(cheng)(cheng)成(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)。并(bing)且，《獸(shou)藥典(dian)》指(zhi)(zhi)出(chu)(chu)(chu)(chu)在(zai)(zai)(zai)【豬(zhu)(zhu)(zhu)(zhu)支(zhi)原(yuan)體(ti)(ti)肺炎(yan)(yan)微(wei)量(liang)(liang)(liang)(liang)間(jian)接(jie)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)凝(ning)試(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)】和(he)(he)【豬(zhu)(zhu)(zhu)(zhu)支(zhi)氣管敗血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)波氏(shi)桿(gan)菌(jun)(jun)(jun)(jun)(jun)凝(ning)集試(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)】中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)需(xu)(xu)要(yao)(yao)(yao)(yao)(yao)陰(yin)性(xing)(xing)(xing)(xing)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，即正常(chang)(chang)(chang)豬(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。 在(zai)(zai)(zai)豬(zhu)(zhu)(zhu)(zhu)瘟(wen)疫(yi)苗(miao)(miao)等(deng)(deng)(deng)其(qi)(qi)他(ta)豬(zhu)(zhu)(zhu)(zhu)病(bing)毒疫(yi)苗(miao)(miao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)豬(zhu)(zhu)(zhu)(zhu)腎細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)（PK-15）或(huo)(huo)(huo)豬(zhu)(zhu)(zhu)(zhu)睪丸細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)（ST）進(jin)行(xing)病(bing)毒的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)繁殖。其(qi)(qi)培(pei)(pei)養(yang)液(ye)(ye)除(chu)(chu)添(tian)加(jia)(jia)MEM外(wai)，常(chang)(chang)(chang)添(tian)加(jia)(jia)新生(sheng)(sheng)(sheng)(sheng)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。但(dan)從(cong)品系相(xiang)同的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)角度(du)(du)來看，豬(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)更為(wei)合(he)(he)(he)適。 實際上(shang)早在(zai)(zai)(zai)1989年(nian)(nian)，國外(wai)就建議(yi)用(yong)(yong)(yong)(yong)(yong)豬(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)取代(dai)(dai)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)培(pei)(pei)養(yang)制(zhi)造(zao)非(fei)(fei)洲豬(zhu)(zhu)(zhu)(zhu)瘟(wen)病(bing)毒的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)（見《動物(wu)(wu)(wu)檢(jian)疫(yi)》 1991年(nian)(nian)01期《豬(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)抗體(ti)(ti)引起(qi)非(fei)(fei)洲豬(zhu)(zhu)(zhu)(zhu)瘟(wen)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)學(xue)診斷(duan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)假(jia)(jia)陽性(xing)(xing)(xing)(xing)反(fan)(fan)(fan)應(ying)(ying)》），原(yuan)因(yin)(yin)(yin)是(shi)(shi)(shi)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)會(hui)干(gan)擾(rao)非(fei)(fei)洲豬(zhu)(zhu)(zhu)(zhu)瘟(wen)抗體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)測(ce)，造(zao)成(cheng)(cheng)假(jia)(jia)陽性(xing)(xing)(xing)(xing)反(fan)(fan)(fan)應(ying)(ying)。
豚(tun)(tun)鼠(shu)(shu)(shu)(shu)(shu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)（去紅細(xi)(xi)胞(bao)(bao)、無(wu)菌過(guo)(guo)濾(lv)）血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)注(zhu)(zhu)意事項：防(fang)止(zhi)(zhi)發病免(mian)疫血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)多(duo)(duo)(duo)用(yong)(yong)(yong)(yong)(yong)馬、牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)制備，馬、牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)對(dui)(dui)其(qi)它動(dong)(dong)(dong)物(wu)(wu)(wu)來(lai)說，也(ye)具(ju)有(you)(you)(you)抗原性(xing)(xing)，有(you)(you)(you)引(yin)起(qi)(qi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)可(ke)(ke)能(neng)(neng)，因(yin)(yin)(yin)此(ci)(ci)，使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)免(mian)疫血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)要(yao)(yao)(yao)(yao)注(zhu)(zhu)意防(fang)止(zhi)(zhi)引(yin)起(qi)(qi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病，預(yu)防(fang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)(zhu)要(yao)(yao)(yao)(yao)措施是(shi)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)提純的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)制品，不(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)合(he)(he)格的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)品；另外，不(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)有(you)(you)(you)急功近利的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)想法(fa)，要(yao)(yao)(yao)(yao)按(an)照(zhao)要(yao)(yao)(yao)(yao)求劑(ji)量(liang)(liang)(liang)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)，一(yi)(yi)(yi)次(ci)(ci)用(yong)(yong)(yong)(yong)(yong)量(liang)(liang)(liang)不(bu)(bu)(bu)(bu)(bu)可(ke)(ke)過(guo)(guo)大(da)(da)。大(da)(da)鼠(shu)(shu)(shu)(shu)(shu)小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)取(qu)(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)操作(zuo)(zuo)(zuo)(zuo)(zuo)步驟：1.剪尾(wei)(wei)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue) 小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)每(mei)次(ci)(ci)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)0.1ml，大(da)(da)鼠(shu)(shu)(shu)(shu)(shu)每(mei)次(ci)(ci)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)0.3-0.5ml左(zuo)(zuo)手(shou)(shou)(shou)拇(mu)指和(he)(he)(he)(he)(he)食(shi)指從(cong)背部(bu)(bu)捉(zhuo)住鼠(shu)(shu)(shu)(shu)(shu)頸(jing)部(bu)(bu)皮(pi)膚(fu)(fu)(fu)，將(jiang)鼠(shu)(shu)(shu)(shu)(shu)頭朝下(xia)(xia)(xia)，鼠(shu)(shu)(shu)(shu)(shu)保定(ding)后(hou)(hou)(hou)(hou)(hou)將(jiang)其(qi)尾(wei)(wei)巴置于50℃熱水(shui)(shui)(shui)中(zhong)(zhong)(zhong)(zhong)浸泡數分(fen)(fen)鐘，使(shi)(shi)(shi)尾(wei)(wei)部(bu)(bu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管(guan)(guan)充(chong)(chong)(chong)盈。擦干(gan)尾(wei)(wei)部(bu)(bu)，再(zai)用(yong)(yong)(yong)(yong)(yong)剪刀或刀片剪去尾(wei)(wei)尖(jian)(jian)1-2mm，用(yong)(yong)(yong)(yong)(yong)試管(guan)(guan)接(jie)(jie)流(liu)出(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)，同(tong)時自尾(wei)(wei)根部(bu)(bu)向(xiang)尾(wei)(wei)尖(jian)(jian)按(an)-摩。取(qu)(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后(hou)(hou)(hou)(hou)(hou)用(yong)(yong)(yong)(yong)(yong)棉球(qiu)壓(ya)(ya)(ya)榨(zha)止(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)并(bing)用(yong)(yong)(yong)(yong)(yong)6%液(ye)(ye)(ye)體(ti)(ti)火棉膠涂(tu)在傷口處(chu)(chu)(chu)止(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。 2.去除眼(yan)(yan)球(qiu)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)0.5-1ml左(zuo)(zuo)手(shou)(shou)(shou)捉(zhuo)住小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)頸(jing)部(bu)(bu)皮(pi)膚(fu)(fu)(fu)，輕(qing)壓(ya)(ya)(ya)在實(shi)(shi)驗(yan)(yan)臺上，取(qu)(qu)(qu)側(ce)臥位(wei)，左(zuo)(zuo)手(shou)(shou)(shou)食(shi)指盡(jin)量(liang)(liang)(liang)將(jiang)小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)眼(yan)(yan)周(zhou)皮(pi)膚(fu)(fu)(fu)往頸(jing)后(hou)(hou)(hou)(hou)(hou)壓(ya)(ya)(ya)，使(shi)(shi)(shi)眼(yan)(yan)球(qiu)杰出(chu)(chu)。用(yong)(yong)(yong)(yong)(yong)眼(yan)(yan)科彎鑷迅速夾去眼(yan)(yan)球(qiu)，將(jiang)鼠(shu)(shu)(shu)(shu)(shu)倒立，用(yong)(yong)(yong)(yong)(yong)器(qi)皿接(jie)(jie)住流(liu)出(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)。采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)完(wan)畢立即(ji)(ji)用(yong)(yong)(yong)(yong)(yong)紗布壓(ya)(ya)(ya)榨(zha)止(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。大(da)(da)鼠(shu)(shu)(shu)(shu)(shu)少用(yong)(yong)(yong)(yong)(yong)。 3.心(xin)臟采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)0.5-0.6ml，大(da)(da)鼠(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)1-1.5ml鼠(shu)(shu)(shu)(shu)(shu)仰臥位(wei)固定(ding)，剪去胸前區(qu)被毛(mao)(mao)(mao)，皮(pi)膚(fu)(fu)(fu)消毒后(hou)(hou)(hou)(hou)(hou)，用(yong)(yong)(yong)(yong)(yong)左(zuo)(zuo)手(shou)(shou)(shou)食(shi)指在左(zuo)(zuo)側(ce)第(di)3-4肋(lei)間(jian)觸(chu)摸到(dao)心(xin)搏(bo)處(chu)(chu)(chu)，右(you)手(shou)(shou)(shou)持(chi)(chi)帶(dai)有(you)(you)(you)4-5號針頭的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)注(zhu)(zhu)射器(qi)，選擇心(xin)搏(bo)zui強處(chu)(chu)(chu)穿刺(ci)(ci)(ci)，當刺(ci)(ci)(ci)中(zhong)(zhong)(zhong)(zhong)心(xin)臟時，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)會(hui)主(zhu)(zhu)(zhu)動(dong)(dong)(dong)進(jin)入(ru)(ru)注(zhu)(zhu)射器(qi)。 4.斷(duan)(duan)頭采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.8-1.2ml，大(da)(da)鼠(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)5-10ml左(zuo)(zuo)手(shou)(shou)(shou)拇(mu)指和(he)(he)(he)(he)(he)食(shi)指從(cong)背部(bu)(bu)捉(zhuo)住鼠(shu)(shu)(shu)(shu)(shu)頸(jing)部(bu)(bu)皮(pi)膚(fu)(fu)(fu)，將(jiang)鼠(shu)(shu)(shu)(shu)(shu)頭朝下(xia)(xia)(xia)，右(you)手(shou)(shou)(shou)用(yong)(yong)(yong)(yong)(yong)剪刀剪斷(duan)(duan)鼠(shu)(shu)(shu)(shu)(shu)頸(jing)部(bu)(bu)約1/2-4/5，讓(rang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)流(liu)入(ru)(ru)試管(guan)(guan)。 5.眼(yan)(yan)眶靜(jing)(jing)脈(mo)(mo)(mo)(mo)叢采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)為(wei)(wei)(wei)(wei)0.2-0.4ml，大(da)(da)鼠(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)為(wei)(wei)(wei)(wei)0.4-0.8ml取(qu)(qu)(qu)內徑為(wei)(wei)(wei)(wei)1.0-1.5mm的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)玻璃毛(mao)(mao)(mao)細(xi)(xi)管(guan)(guan)，臨用(yong)(yong)(yong)(yong)(yong)前折斷(duan)(duan)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)1～1.5cm長(chang)(chang)(chang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)毛(mao)(mao)(mao)細(xi)(xi)管(guan)(guan)段(duan)，浸入(ru)(ru)1％肝(gan)素(su)溶液(ye)(ye)(ye)中(zhong)(zhong)(zhong)(zhong)，干(gan)燥后(hou)(hou)(hou)(hou)(hou)用(yong)(yong)(yong)(yong)(yong)。取(qu)(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)時左(zuo)(zuo)手(shou)(shou)(shou)捉(zhuo)住鼠(shu)(shu)(shu)(shu)(shu)兩耳之(zhi)(zhi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)頸(jing)背部(bu)(bu)皮(pi)膚(fu)(fu)(fu)以(yi)(yi)固定(ding)頭部(bu)(bu)，悄(qiao)悄(qiao)向(xiang)下(xia)(xia)(xia)壓(ya)(ya)(ya)榨(zha)頸(jing)部(bu)(bu)兩邊，引(yin)起(qi)(qi)頭部(bu)(bu)靜(jing)(jing)脈(mo)(mo)(mo)(mo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)回流(liu)困難(nan)使(shi)(shi)(shi)眼(yan)(yan)眶靜(jing)(jing)脈(mo)(mo)(mo)(mo)叢充(chong)(chong)(chong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)，右(you)手(shou)(shou)(shou)持(chi)(chi)毛(mao)(mao)(mao)細(xi)(xi)管(guan)(guan)由內眥部(bu)(bu)刺(ci)(ci)(ci)進(jin)結(jie)膜，再(zai)悄(qiao)悄(qiao)向(xiang)眼(yan)(yan)底(di)部(bu)(bu)方向(xiang)推(tui)進(jin)，悄(qiao)悄(qiao)旋(xuan)轉毛(mao)(mao)(mao)細(xi)(xi)管(guan)(guan)以(yi)(yi)劃破靜(jing)(jing)脈(mo)(mo)(mo)(mo)叢，讓(rang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)順毛(mao)(mao)(mao)細(xi)(xi)管(guan)(guan)流(liu)出(chu)(chu)，接(jie)(jie)收入(ru)(ru)事前準備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)容器(qi)中(zhong)(zhong)(zhong)(zhong)。采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后(hou)(hou)(hou)(hou)(hou)紗布輕(qing)壓(ya)(ya)(ya)眼(yan)(yan)部(bu)(bu)止(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)、大(da)(da)鼠(shu)(shu)(shu)(shu)(shu)、豚(tun)(tun)鼠(shu)(shu)(shu)(shu)(shu)及家兔均(jun)可(ke)(ke)采(cai)(cai)(cai)(cai)納(na)此(ci)(ci)法(fa)取(qu)(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。刺(ci)(ci)(ci)入(ru)(ru)深度(du)小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)為(wei)(wei)(wei)(wei)2-3mm，可(ke)(ke)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.2-0.3ml；大(da)(da)鼠(shu)(shu)(shu)(shu)(shu)為(wei)(wei)(wei)(wei)4-5mm，可(ke)(ke)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.4-0.8ml。豚(tun)(tun)鼠(shu)(shu)(shu)(shu)(shu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)（去紅細(xi)(xi)胞(bao)(bao)、無(wu)菌過(guo)(guo)濾(lv)）Tips：1. 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保存(cun)(cun)(cun)：在-15℃以(yi)(yi)下(xia)(xia)(xia)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)(you)效期為(wei)(wei)(wei)(wei)5年(nian)，4℃可(ke)(ke)保存(cun)(cun)(cun)數周(zhou)，不(bu)(bu)(bu)(bu)(bu)宜(yi)室溫(wen)保存(cun)(cun)(cun)，不(bu)(bu)(bu)(bu)(bu)宜(yi)反復(fu)凍(dong)(dong)融。配制成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)完(wan)全培養(yang)(yang)基后(hou)(hou)(hou)(hou)(hou)應(ying)在四周(zhou)內用(yong)(yong)(yong)(yong)(yong)完(wan)，一(yi)(yi)(yi)瓶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)如無(wu)法(fa)一(yi)(yi)(yi)次(ci)(ci)用(yong)(yong)(yong)(yong)(yong)完(wan)應(ying)無(wu)菌分(fen)(fen)裝成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)恰當數量(liang)(liang)(liang)并(bing)冷凍(dong)(dong)保存(cun)(cun)(cun)。2、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)絮(xu)狀(zhuang)(zhuang)(zhuang)(zhuang)沉(chen)淀(dian)物(wu)(wu)(wu)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)多(duo)(duo)(duo)種(zhong)蛋(dan)(dan)白(bai)(bai)質(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混(hun)合(he)(he)物(wu)(wu)(wu)，在凍(dong)(dong)融過(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)會(hui)出(chu)(chu)現(xian)絮(xu)狀(zhuang)(zhuang)(zhuang)(zhuang)沉(chen)淀(dian)物(wu)(wu)(wu)，主(zhu)(zhu)(zhu)要(yao)(yao)(yao)(yao)原因(yin)(yin)(yin)是(shi)由于血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)脂蛋(dan)(dan)白(bai)(bai)和(he)(he)(he)(he)(he)纖維蛋(dan)(dan)白(bai)(bai)變性(xing)(xing)所造(zao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)。但并(bing)不(bu)(bu)(bu)(bu)(bu)影(ying)(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本身的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)量(liang)(liang)(liang)和(he)(he)(he)(he)(he)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。如需(xu)(xu)去除可(ke)(ke)先(xian)400g離心(xin)取(qu)(qu)(qu)上清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)配制成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)完(wan)全培養(yang)(yang)基再(zai)過(guo)(guo)濾(lv)即(ji)(ji)可(ke)(ke)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。3、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)凍(dong)(dong)：合(he)(he)理(li)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)凍(dong)(dong)方式可(ke)(ke)以(yi)(yi)更好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保護(hu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)量(liang)(liang)(liang)并(bing)可(ke)(ke)以(yi)(yi)在＊大(da)(da)程(cheng)度(du)上減少絮(xu)狀(zhuang)(zhuang)(zhuang)(zhuang)沉(chen)淀(dian)物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)低(di)溫(wen)冰箱(xiang)中(zhong)(zhong)(zhong)(zhong)取(qu)(qu)(qu)出(chu)(chu)后(hou)(hou)(hou)(hou)(hou)，經(jing)4℃冰箱(xiang)、室溫(wen)、37℃水(shui)(shui)(shui)浴(yu)逐(zhu)(zhu)步規則搖晃均(jun)勻(yun)融解(jie)，直(zhi)至(zhi)(zhi)完(wan)全融解(jie)后(hou)(hou)(hou)(hou)(hou)再(zai)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。4、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱滅(mie)活：將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)嚴(yan)格56℃水(shui)(shui)(shui)浴(yu)均(jun)勻(yun)加溫(wen)30分(fen)(fen)鐘可(ke)(ke)以(yi)(yi)滅(mie)活血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補體(ti)(ti)系統。是(shi)不(bu)(bu)(bu)(bu)(bu)是(shi)所有(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)途的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都需(xu)(xu)要(yao)(yao)(yao)(yao)熱滅(mie)活呢？對(dui)(dui)大(da)(da)多(duo)(duo)(duo)數細(xi)(xi)胞(bao)(bao)來(lai)說是(shi)不(bu)(bu)(bu)(bu)(bu)需(xu)(xu)要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。激活的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補體(ti)(ti)能(neng)(neng)參與溶解(jie)細(xi)(xi)胞(bao)(bao)，刺(ci)(ci)(ci)激光滑(hua)肌(ji)收縮(suo)，細(xi)(xi)胞(bao)(bao)和(he)(he)(he)(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)(xiao)板釋放(fang)組(zu)(zu)胺(an)，激活淋巴細(xi)(xi)胞(bao)(bao)和(he)(he)(he)(he)(he)巨噬細(xi)(xi)胞(bao)(bao)。因(yin)(yin)(yin)此(ci)(ci)在免(mian)疫學(xue)(xue)研(yan)究，培養(yang)(yang)ES細(xi)(xi)胞(bao)(bao)，昆蟲(chong)細(xi)(xi)胞(bao)(bao)和(he)(he)(he)(he)(he)平(ping)滑(hua)肌(ji)肉細(xi)(xi)胞(bao)(bao)時，建議使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)熱滅(mie)活血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，在正(zheng)常情況下(xia)(xia)(xia)加熱處(chu)(chu)(chu)理(li)對(dui)(dui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)促(cu)細(xi)(xi)胞(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)效應(ying)無(wu)明(ming)顯促(cu)進(jin)作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)，甚(shen)至(zhi)(zhi)會(hui)因(yin)(yin)(yin)為(wei)(wei)(wei)(wei)加熱處(chu)(chu)(chu)理(li)影(ying)(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)量(liang)(liang)(liang)。5、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)“小(xiao)(xiao)(xiao)黑點”現(xian)象：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在經(jing)過(guo)(guo)一(yi)(yi)(yi)段(duan)時間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低(di)溫(wen)凍(dong)(dong)存(cun)(cun)(cun)再(zai)融解(jie)后(hou)(hou)(hou)(hou)(hou)，在正(zheng)常情況下(xia)(xia)(xia)會(hui)出(chu)(chu)現(xian)絮(xu)狀(zhuang)(zhuang)(zhuang)(zhuang)蛋(dan)(dan)白(bai)(bai)沉(chen)淀(dian)。但往往有(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)戶反應(ying)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)“小(xiao)(xiao)(xiao)黑點”現(xian)象，認(ren)為(wei)(wei)(wei)(wei)是(shi)微生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)污染(ran)。實(shi)(shi)驗(yan)(yan)發現(xian)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在37℃環境放(fang)置時間(jian)過(guo)(guo)長(chang)(chang)(chang)或經(jing)過(guo)(guo)加熱滅(mie)活處(chu)(chu)(chu)理(li)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)沉(chen)淀(dian)物(wu)(wu)(wu)往往會(hui)呈增多(duo)(duo)(duo)趨勢，這(zhe)些沉(chen)淀(dian)物(wu)(wu)(wu)在倒置顯微鏡下(xia)(xia)(xia)觀察，像是(shi)“小(xiao)(xiao)(xiao)黑點”，讓(rang)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)者誤以(yi)(yi)為(wei)(wei)(wei)(wei)是(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)遭受微生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)污染(ran)并(bing)在分(fen)(fen)裂增殖。因(yin)(yin)(yin)此(ci)(ci)如無(wu)必要(yao)(yao)(yao)(yao)，完(wan)全可(ke)(ke)以(yi)(yi)不(bu)(bu)(bu)(bu)(bu)進(jin)行熱滅(mie)活處(chu)(chu)(chu)理(li)并(bing)保證(zheng)(zheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低(di)溫(wen)保存(cun)(cun)(cun)。實(shi)(shi)驗(yan)(yan)證(zheng)(zheng)明(ming)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時注(zhu)(zhu)意以(yi)(yi)下(xia)(xia)(xia)幾點事項不(bu)(bu)(bu)(bu)(bu)但可(ke)(ke)以(yi)(yi)保證(zheng)(zheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)量(liang)(liang)(liang)并(bing)能(neng)(neng)給使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)者帶(dai)來(lai)方便：◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)在-15℃以(yi)(yi)下(xia)(xia)(xia)低(di)溫(wen)保存(cun)(cun)(cun)。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)(yi)(yi)瓶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)(yi)(yi)次(ci)(ci)無(wu)法(fa)用(yong)(yong)(yong)(yong)(yong)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)分(fen)(fen)裝凍(dong)(dong)存(cun)(cun)(cun)，分(fen)(fen)次(ci)(ci)用(yong)(yong)(yong)(yong)(yong)完(wan)。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)按(an)-15℃以(yi)(yi)下(xia)(xia)(xia)至(zhi)(zhi)4℃至(zhi)(zhi)室溫(wen)至(zhi)(zhi)37℃逐(zhu)(zhu)步均(jun)勻(yun)解(jie)凍(dong)(dong)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。◎若(ruo)非必要(yao)(yao)(yao)(yao)，無(wu)須(xu)(xu)進(jin)行熱滅(mie)活。若(ruo)必須(xu)(xu)做血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)熱滅(mie)活的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，須(xu)(xu)嚴(yan)格控制在56℃水(shui)(shui)(shui)浴(yu)30分(fen)(fen)鐘均(jun)勻(yun)加熱滅(mie)活。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)定(ding)義血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)指血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)除去纖維蛋(dan)(dan)白(bai)(bai)原后(hou)(hou)(hou)(hou)(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠狀(zhuang)(zhuang)(zhuang)(zhuang)液(ye)(ye)(ye)體(ti)(ti)。每(mei)100ml人(ren)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)含(han)(han)有(you)(you)(you)蛋(dan)(dan)白(bai)(bai)質(zhi)(zhi)6-8g，其(qi)中(zhong)(zhong)(zhong)(zhong)主(zhu)(zhu)(zhu)要(yao)(yao)(yao)(yao)是(shi)白(bai)(bai)蛋(dan)(dan)白(bai)(bai)和(he)(he)(he)(he)(he)球(qiu)蛋(dan)(dan)白(bai)(bai)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)不(bu)(bu)(bu)(bu)(bu)會(hui)凝(ning)固。有(you)(you)(you)免(mian)疫，維持(chi)(chi)酸(suan)堿(jian)平(ping)衡和(he)(he)(he)(he)(he)滲透壓(ya)(ya)(ya)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)蛋(dan)(dan)白(bai)(bai)質(zhi)(zhi)亦可(ke)(ke)儲(chu)備供(gong)給機(ji)體(ti)(ti)蛋(dan)(dan)白(bai)(bai)質(zhi)(zhi)不(bu)(bu)(bu)(bu)(bu)足(zu)時的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)用(yong)(yong)(yong)(yong)(yong)。人(ren)和(he)(he)(he)(he)(he)動(dong)(dong)(dong)物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)常用(yong)(yong)(yong)(yong)(yong)以(yi)(yi)進(jin)行各種(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)學(xue)(xue)試驗(yan)(yan)，幫助診斷(duan)(duan)疾(ji)病。含(han)(han)抗體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)作(zuo)(zuo)(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)預(yu)防(fang)或治(zhi)療疾(ji)病之(zhi)(zhi)用(yong)(yong)(yong)(yong)(yong) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)(zhu)要(yao)(yao)(yao)(yao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)由血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)去除纖維蛋(dan)(dan)白(bai)(bai)原而形成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)種(zhong)很復(fu)雜的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混(hun)合(he)(he)物(wu)(wu)(wu)，其(qi)組(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)份雖(sui)大(da)(da)部(bu)(bu)分(fen)(fen)已知，但還有(you)(you)(you)一(yi)(yi)(yi)部(bu)(bu)分(fen)(fen)尚不(bu)(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚(chu)，且(qie)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)及含(han)(han)量(liang)(liang)(liang)常隨供(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)(dong)(dong)物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)別、年(nian)齡、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)條件和(he)(he)(he)(he)(he)營養(yang)(yang)條件不(bu)(bu)(bu)(bu)(bu)同(tong)而異(yi)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)中(zhong)(zhong)(zhong)(zhong)不(bu)(bu)(bu)(bu)(bu)含(han)(han)纖維蛋(dan)(dan)白(bai)(bai)原的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠狀(zhuang)(zhuang)(zhuang)(zhuang)液(ye)(ye)(ye)體(ti)(ti),具(ju)有(you)(you)(you)維持(chi)(chi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正(zheng)常粘度(du)、酸(suan)堿(jian)度(du)、滲透壓(ya)(ya)(ya)等作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。它主(zhu)(zhu)(zhu)要(yao)(yao)(yao)(yao)由水(shui)(shui)(shui)和(he)(he)(he)(he)(he)各種(zhong)化學(xue)(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)組(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)，這(zhe)些化學(xue)(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)包括白(bai)(bai)蛋(dan)(dan)白(bai)(bai)，α1、α2、β、γ-球(qiu)蛋(dan)(dan)白(bai)(bai)，甘(gan)油三酯(zhi)，總膽(dan)固醇，谷丙轉氨酶等。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)含(han)(han)有(you)(you)(you)各種(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)蛋(dan)(dan)白(bai)(bai)、多(duo)(duo)(duo)肽、脂肪、碳水(shui)(shui)(shui)化合(he)(he)物(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)子、激素(su)、無(wu)機(ji)物(wu)(wu)(wu)等，這(zhe)些物(wu)(wu)(wu)質(zhi)(zhi)對(dui)(dui)促(cu)進(jin)細(xi)(xi)胞(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)或抑制生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)活性(xing)(xing)是(shi)達到(dao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)平(ping)衡的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。對(dui)(dui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)和(he)(he)(he)(he)(he)作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)研(yan)究雖(sui)有(you)(you)(you)很大(da)(da)進(jin)展(zhan)，但仍(reng)存(cun)(cun)(cun)在一(yi)(yi)(yi)些問題(ti)。主(zhu)(zhu)(zhu)要(yao)(yao)(yao)(yao)是(shi)：第(di)一(yi)(yi)(yi)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)份可(ke)(ke)能(neng)(neng)有(you)(you)(you)幾百(bai)種(zhong)之(zhi)(zhi)多(duo)(duo)(duo)，對(dui)(dui)其(qi)準確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)份、含(han)(han)量(liang)(liang)(liang)及其(qi)作(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)機(ji)制仍(reng)不(bu)(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚(chu)，尤其(qi)是(shi)對(dui)(dui)其(qi)中(zhong)(zhong)(zhong)(zhong)一(yi)(yi)(yi)些多(duo)(duo)(duo)肽類(lei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)子、激素(su)和(he)(he)(he)(he)(he)脂類(lei)等尚未充(chong)(chong)(chong)分(fen)(fen)認(ren)識，這(zhe)給研(yan)究工(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)帶(dai)來(lai)許多(duo)(duo)(duo)困難(nan)；第(di)二：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都是(shi)批量(liang)(liang)(liang)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)，各批量(liang)(liang)(liang)之(zhi)(zhi)間(jian)差(cha)異(yi)很大(da)(da)，而且(qie)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)保存(cun)(cun)(cun)期至(zhi)(zhi)多(duo)(duo)(duo)一(yi)(yi)(yi)年(nian)，因(yin)(yin)(yin)此(ci)(ci)，要(yao)(yao)(yao)(yao)保證(zheng)(zheng)每(mei)批血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)相似性(xing)(xing)極(ji)為(wei)(wei)(wei)(wei)困難(nan)，從(cong)而使(shi)(shi)(shi)實(shi)(shi)驗(yan)(yan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標準化和(he)(he)(he)(he)(he)連續性(xing)(xing)受到(dao)限制；第(di)三：不(bu)(bu)(bu)(bu)(bu)能(neng)(neng)排(pai)除血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)含(han)(han)有(you)(you)(you)易變物(wu)(wu)(wu)質(zhi)(zhi)，這(zhe)被認(ren)為(wei)(wei)(wei)(wei)是(shi)“瓶中(zhong)(zhong)(zhong)(zhong)惡化”的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原因(yin)(yin)(yin)之(zhi)(zhi)一(yi)(yi)(yi)
小(xiao)鼠(shu)(shu)(shu)(shu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)（去(qu)紅細(xi)(xi)(xi)(xi)胞(bao)(bao)、無(wu)(wu)(wu)菌(jun)過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)）血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)使(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)注意事(shi)(shi)項(xiang)：防(fang)(fang)止發病(bing)(bing)免(mian)疫血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)多(duo)(duo)(duo)(duo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)馬、牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)制(zhi)備(bei)，馬、牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)對(dui)其(qi)它動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)來(lai)說(shuo)，也具(ju)有(you)(you)(you)(you)(you)抗(kang)原(yuan)(yuan)性(xing)(xing)(xing)，有(you)(you)(you)(you)(you)引起血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病(bing)(bing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)可(ke)(ke)(ke)(ke)(ke)(ke)能(neng)(neng)，因(yin)(yin)此，使(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)免(mian)疫血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)要(yao)(yao)(yao)注意防(fang)(fang)止引起血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病(bing)(bing)，預防(fang)(fang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要(yao)(yao)(yao)措施是(shi)(shi)(shi)(shi)使(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)提純(chun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)品，不(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)合(he)格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)品；另外，不(bu)(bu)要(yao)(yao)(yao)有(you)(you)(you)(you)(you)急功近(jin)利的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)想法，要(yao)(yao)(yao)按(an)(an)照要(yao)(yao)(yao)求劑(ji)量(liang)(liang)(liang)(liang)(liang)使(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，一(yi)次(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)量(liang)(liang)(liang)(liang)(liang)不(bu)(bu)可(ke)(ke)(ke)(ke)(ke)(ke)過(guo)(guo)(guo)(guo)(guo)大(da)(da)(da)。大(da)(da)(da)鼠(shu)(shu)(shu)(shu)小(xiao)鼠(shu)(shu)(shu)(shu)取(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)操作(zuo)步(bu)(bu)驟：1.剪尾采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue) 小(xiao)鼠(shu)(shu)(shu)(shu)每次(ci)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)0.1ml，大(da)(da)(da)鼠(shu)(shu)(shu)(shu)每次(ci)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)0.3-0.5ml左(zuo)(zuo)手(shou)拇(mu)指(zhi)(zhi)和(he)(he)(he)(he)(he)食指(zhi)(zhi)從背(bei)部(bu)(bu)捉(zhuo)住鼠(shu)(shu)(shu)(shu)頸部(bu)(bu)皮膚，將(jiang)鼠(shu)(shu)(shu)(shu)頭(tou)朝下(xia)，鼠(shu)(shu)(shu)(shu)保(bao)定(ding)后(hou)(hou)將(jiang)其(qi)尾巴置(zhi)于50℃熱(re)(re)水(shui)中(zhong)(zhong)(zhong)浸泡數分(fen)(fen)(fen)(fen)(fen)鐘，使(shi)(shi)(shi)(shi)(shi)尾部(bu)(bu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管(guan)(guan)充盈。擦干尾部(bu)(bu)，再用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)剪刀(dao)或(huo)(huo)刀(dao)片剪去(qu)尾尖(jian)1-2mm，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)(shi)管(guan)(guan)接流(liu)出的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)，同時(shi)自尾根部(bu)(bu)向(xiang)尾尖(jian)按(an)(an)-摩(mo)。取(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后(hou)(hou)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)棉(mian)球(qiu)(qiu)壓(ya)榨(zha)(zha)止血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)并(bing)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)6%液(ye)(ye)(ye)體(ti)(ti)火棉(mian)膠涂在(zai)傷(shang)口處(chu)(chu)(chu)止血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。 2.去(qu)除眼(yan)球(qiu)(qiu)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)鼠(shu)(shu)(shu)(shu)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)0.5-1ml左(zuo)(zuo)手(shou)捉(zhuo)住小(xiao)鼠(shu)(shu)(shu)(shu)頸部(bu)(bu)皮膚，輕壓(ya)在(zai)實(shi)驗(yan)臺上(shang)，取(qu)(qu)側臥位，左(zuo)(zuo)手(shou)食指(zhi)(zhi)盡量(liang)(liang)(liang)(liang)(liang)將(jiang)小(xiao)鼠(shu)(shu)(shu)(shu)眼(yan)周(zhou)皮膚往頸后(hou)(hou)壓(ya)，使(shi)(shi)(shi)(shi)(shi)眼(yan)球(qiu)(qiu)杰出。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)眼(yan)科彎鑷迅速夾去(qu)眼(yan)球(qiu)(qiu)，將(jiang)鼠(shu)(shu)(shu)(shu)倒(dao)立(li)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)器(qi)皿接住流(liu)出的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)。采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)完(wan)(wan)畢立(li)即用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)紗布壓(ya)榨(zha)(zha)止血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。大(da)(da)(da)鼠(shu)(shu)(shu)(shu)少用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。 3.心(xin)臟采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)鼠(shu)(shu)(shu)(shu)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)0.5-0.6ml，大(da)(da)(da)鼠(shu)(shu)(shu)(shu)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)1-1.5ml鼠(shu)(shu)(shu)(shu)仰臥位固(gu)定(ding)，剪去(qu)胸(xiong)前(qian)區被毛(mao)，皮膚消毒后(hou)(hou)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)左(zuo)(zuo)手(shou)食指(zhi)(zhi)在(zai)左(zuo)(zuo)側第3-4肋間(jian)(jian)觸摸到心(xin)搏處(chu)(chu)(chu)，右手(shou)持(chi)帶(dai)有(you)(you)(you)(you)(you)4-5號針頭(tou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)注射(she)器(qi)，選擇心(xin)搏zui強處(chu)(chu)(chu)穿刺(ci)(ci)，當刺(ci)(ci)中(zhong)(zhong)(zhong)心(xin)臟時(shi)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)會(hui)主(zhu)動(dong)(dong)進(jin)(jin)(jin)(jin)入(ru)(ru)注射(she)器(qi)。 4.斷(duan)(duan)頭(tou)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)鼠(shu)(shu)(shu)(shu)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.8-1.2ml，大(da)(da)(da)鼠(shu)(shu)(shu)(shu)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)5-10ml左(zuo)(zuo)手(shou)拇(mu)指(zhi)(zhi)和(he)(he)(he)(he)(he)食指(zhi)(zhi)從背(bei)部(bu)(bu)捉(zhuo)住鼠(shu)(shu)(shu)(shu)頸部(bu)(bu)皮膚，將(jiang)鼠(shu)(shu)(shu)(shu)頭(tou)朝下(xia)，右手(shou)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)剪刀(dao)剪斷(duan)(duan)鼠(shu)(shu)(shu)(shu)頸部(bu)(bu)約1/2-4/5，讓(rang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)流(liu)入(ru)(ru)試(shi)(shi)管(guan)(guan)。 5.眼(yan)眶靜(jing)(jing)脈(mo)叢(cong)(cong)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)鼠(shu)(shu)(shu)(shu)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)為(wei)0.2-0.4ml，大(da)(da)(da)鼠(shu)(shu)(shu)(shu)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)為(wei)0.4-0.8ml取(qu)(qu)內徑為(wei)1.0-1.5mm的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)玻璃毛(mao)細(xi)(xi)(xi)(xi)管(guan)(guan)，臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)折斷(duan)(duan)成(cheng)(cheng)(cheng)(cheng)(cheng)1～1.5cm長的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)毛(mao)細(xi)(xi)(xi)(xi)管(guan)(guan)段(duan)，浸入(ru)(ru)1％肝素(su)溶(rong)液(ye)(ye)(ye)中(zhong)(zhong)(zhong)，干燥后(hou)(hou)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。取(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)時(shi)左(zuo)(zuo)手(shou)捉(zhuo)住鼠(shu)(shu)(shu)(shu)兩(liang)耳之(zhi)間(jian)(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)頸背(bei)部(bu)(bu)皮膚以(yi)固(gu)定(ding)頭(tou)部(bu)(bu)，悄(qiao)(qiao)(qiao)悄(qiao)(qiao)(qiao)向(xiang)下(xia)壓(ya)榨(zha)(zha)頸部(bu)(bu)兩(liang)邊，引起頭(tou)部(bu)(bu)靜(jing)(jing)脈(mo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)回流(liu)困(kun)難使(shi)(shi)(shi)(shi)(shi)眼(yan)眶靜(jing)(jing)脈(mo)叢(cong)(cong)充血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)，右手(shou)持(chi)毛(mao)細(xi)(xi)(xi)(xi)管(guan)(guan)由內眥部(bu)(bu)刺(ci)(ci)進(jin)(jin)(jin)(jin)結膜，再悄(qiao)(qiao)(qiao)悄(qiao)(qiao)(qiao)向(xiang)眼(yan)底部(bu)(bu)方向(xiang)推進(jin)(jin)(jin)(jin)，悄(qiao)(qiao)(qiao)悄(qiao)(qiao)(qiao)旋轉(zhuan)毛(mao)細(xi)(xi)(xi)(xi)管(guan)(guan)以(yi)劃(hua)破靜(jing)(jing)脈(mo)叢(cong)(cong)，讓(rang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)順毛(mao)細(xi)(xi)(xi)(xi)管(guan)(guan)流(liu)出，接收入(ru)(ru)事(shi)(shi)前(qian)準備(bei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)容器(qi)中(zhong)(zhong)(zhong)。采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后(hou)(hou)紗布輕壓(ya)眼(yan)部(bu)(bu)止血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。小(xiao)鼠(shu)(shu)(shu)(shu)、大(da)(da)(da)鼠(shu)(shu)(shu)(shu)、豚鼠(shu)(shu)(shu)(shu)及家(jia)兔(tu)均可(ke)(ke)(ke)(ke)(ke)(ke)采(cai)(cai)(cai)納此法取(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。刺(ci)(ci)入(ru)(ru)深度(du)(du)小(xiao)鼠(shu)(shu)(shu)(shu)為(wei)2-3mm，可(ke)(ke)(ke)(ke)(ke)(ke)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.2-0.3ml；大(da)(da)(da)鼠(shu)(shu)(shu)(shu)為(wei)4-5mm，可(ke)(ke)(ke)(ke)(ke)(ke)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.4-0.8ml。Tips：1. 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)存：在(zai)-15℃以(yi)下(xia)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)(you)(you)(you)效(xiao)期為(wei)5年，4℃可(ke)(ke)(ke)(ke)(ke)(ke)保(bao)存數周(zhou)，不(bu)(bu)宜(yi)室(shi)溫(wen)保(bao)存，不(bu)(bu)宜(yi)反復凍(dong)融(rong)。配制(zhi)成(cheng)(cheng)(cheng)(cheng)(cheng)完(wan)(wan)全(quan)培養基后(hou)(hou)應(ying)在(zai)四周(zhou)內用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)，一(yi)瓶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)如(ru)(ru)無(wu)(wu)(wu)法一(yi)次(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)應(ying)無(wu)(wu)(wu)菌(jun)分(fen)(fen)(fen)(fen)(fen)裝成(cheng)(cheng)(cheng)(cheng)(cheng)恰當數量(liang)(liang)(liang)(liang)(liang)并(bing)冷凍(dong)保(bao)存。2、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)絮(xu)狀沉淀(dian)(dian)物(wu)(wu)(wu)(wu)(wu)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)多(duo)(duo)(duo)(duo)種(zhong)(zhong)(zhong)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)物(wu)(wu)(wu)(wu)(wu)，在(zai)凍(dong)融(rong)過(guo)(guo)(guo)(guo)(guo)程中(zhong)(zhong)(zhong)會(hui)出現絮(xu)狀沉淀(dian)(dian)物(wu)(wu)(wu)(wu)(wu)，主(zhu)要(yao)(yao)(yao)原(yuan)(yuan)因(yin)(yin)是(shi)(shi)(shi)(shi)由于血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)脂(zhi)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)和(he)(he)(he)(he)(he)纖維(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)變(bian)性(xing)(xing)(xing)所(suo)造成(cheng)(cheng)(cheng)(cheng)(cheng)。但(dan)并(bing)不(bu)(bu)影響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本(ben)身的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)使(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。如(ru)(ru)需去(qu)除可(ke)(ke)(ke)(ke)(ke)(ke)先400g離心(xin)取(qu)(qu)上(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)配制(zhi)成(cheng)(cheng)(cheng)(cheng)(cheng)完(wan)(wan)全(quan)培養基再過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)即可(ke)(ke)(ke)(ke)(ke)(ke)使(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。3、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)(jie)(jie)凍(dong)：合(he)理(li)(li)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)(jie)(jie)凍(dong)方式(shi)可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)更好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)護血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)并(bing)可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)在(zai)＊大(da)(da)(da)程度(du)(du)上(shang)減少絮(xu)狀沉淀(dian)(dian)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)生。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從低溫(wen)冰(bing)箱中(zhong)(zhong)(zhong)取(qu)(qu)出后(hou)(hou)，經(jing)4℃冰(bing)箱、室(shi)溫(wen)、37℃水(shui)浴(yu)逐步(bu)(bu)規則搖晃均勻(yun)(yun)(yun)(yun)融(rong)解(jie)(jie)(jie)，直至完(wan)(wan)全(quan)融(rong)解(jie)(jie)(jie)后(hou)(hou)再使(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。4、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱(re)(re)滅(mie)(mie)活(huo)(huo)：將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)嚴格(ge)56℃水(shui)浴(yu)均勻(yun)(yun)(yun)(yun)加(jia)(jia)溫(wen)30分(fen)(fen)(fen)(fen)(fen)鐘可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)滅(mie)(mie)活(huo)(huo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補(bu)體(ti)(ti)系統。是(shi)(shi)(shi)(shi)不(bu)(bu)是(shi)(shi)(shi)(shi)所(suo)有(you)(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)途的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都需要(yao)(yao)(yao)熱(re)(re)滅(mie)(mie)活(huo)(huo)呢？對(dui)大(da)(da)(da)多(duo)(duo)(duo)(duo)數細(xi)(xi)(xi)(xi)胞(bao)(bao)來(lai)說(shuo)是(shi)(shi)(shi)(shi)不(bu)(bu)需要(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。激(ji)(ji)活(huo)(huo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補(bu)體(ti)(ti)能(neng)(neng)參(can)與溶(rong)解(jie)(jie)(jie)細(xi)(xi)(xi)(xi)胞(bao)(bao)，刺(ci)(ci)激(ji)(ji)光滑肌收縮(suo)，細(xi)(xi)(xi)(xi)胞(bao)(bao)和(he)(he)(he)(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)板釋(shi)放組(zu)胺，激(ji)(ji)活(huo)(huo)淋巴細(xi)(xi)(xi)(xi)胞(bao)(bao)和(he)(he)(he)(he)(he)巨噬細(xi)(xi)(xi)(xi)胞(bao)(bao)。因(yin)(yin)此在(zai)免(mian)疫學(xue)研(yan)究(jiu)，培養ES細(xi)(xi)(xi)(xi)胞(bao)(bao)，昆(kun)蟲細(xi)(xi)(xi)(xi)胞(bao)(bao)和(he)(he)(he)(he)(he)平(ping)滑肌肉細(xi)(xi)(xi)(xi)胞(bao)(bao)時(shi)，建議使(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)熱(re)(re)滅(mie)(mie)活(huo)(huo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，在(zai)正(zheng)常(chang)(chang)情況下(xia)加(jia)(jia)熱(re)(re)處(chu)(chu)(chu)理(li)(li)對(dui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)促(cu)細(xi)(xi)(xi)(xi)胞(bao)(bao)生長效(xiao)應(ying)無(wu)(wu)(wu)明(ming)顯(xian)促(cu)進(jin)(jin)(jin)(jin)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，甚至會(hui)因(yin)(yin)為(wei)加(jia)(jia)熱(re)(re)處(chu)(chu)(chu)理(li)(li)影響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)。5、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)“小(xiao)黑點”現象：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)經(jing)過(guo)(guo)(guo)(guo)(guo)一(yi)段(duan)時(shi)間(jian)(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低溫(wen)凍(dong)存再融(rong)解(jie)(jie)(jie)后(hou)(hou)，在(zai)正(zheng)常(chang)(chang)情況下(xia)會(hui)出現絮(xu)狀蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)沉淀(dian)(dian)。但(dan)往往有(you)(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶反應(ying)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)有(you)(you)(you)(you)(you)“小(xiao)黑點”現象，認為(wei)是(shi)(shi)(shi)(shi)微生物(wu)(wu)(wu)(wu)(wu)污染。實(shi)驗(yan)發現血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)37℃環境放置(zhi)時(shi)間(jian)(jian)過(guo)(guo)(guo)(guo)(guo)長或(huo)(huo)經(jing)過(guo)(guo)(guo)(guo)(guo)加(jia)(jia)熱(re)(re)滅(mie)(mie)活(huo)(huo)處(chu)(chu)(chu)理(li)(li)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)沉淀(dian)(dian)物(wu)(wu)(wu)(wu)(wu)往往會(hui)呈(cheng)增多(duo)(duo)(duo)(duo)趨勢，這(zhe)(zhe)些沉淀(dian)(dian)物(wu)(wu)(wu)(wu)(wu)在(zai)倒(dao)置(zhi)顯(xian)微鏡(jing)下(xia)觀(guan)察，像是(shi)(shi)(shi)(shi)“小(xiao)黑點”，讓(rang)使(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)者誤以(yi)為(wei)是(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)遭受微生物(wu)(wu)(wu)(wu)(wu)污染并(bing)在(zai)分(fen)(fen)(fen)(fen)(fen)裂增殖。因(yin)(yin)此如(ru)(ru)無(wu)(wu)(wu)必(bi)(bi)要(yao)(yao)(yao)，完(wan)(wan)全(quan)可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)不(bu)(bu)進(jin)(jin)(jin)(jin)行熱(re)(re)滅(mie)(mie)活(huo)(huo)處(chu)(chu)(chu)理(li)(li)并(bing)保(bao)證(zheng)(zheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低溫(wen)保(bao)存。小(xiao)鼠(shu)(shu)(shu)(shu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)（去(qu)紅細(xi)(xi)(xi)(xi)胞(bao)(bao)、無(wu)(wu)(wu)菌(jun)過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)）實(shi)驗(yan)證(zheng)(zheng)明(ming)使(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)注意以(yi)下(xia)幾(ji)點事(shi)(shi)項(xiang)不(bu)(bu)但(dan)可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)保(bao)證(zheng)(zheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)并(bing)能(neng)(neng)給(gei)使(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)者帶(dai)來(lai)方便(bian)：◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)(ke)(ke)(ke)(ke)在(zai)-15℃以(yi)下(xia)低溫(wen)保(bao)存。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)瓶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)次(ci)無(wu)(wu)(wu)法用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)分(fen)(fen)(fen)(fen)(fen)裝凍(dong)存，分(fen)(fen)(fen)(fen)(fen)次(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)按(an)(an)-15℃以(yi)下(xia)至4℃至室(shi)溫(wen)至37℃逐步(bu)(bu)均勻(yun)(yun)(yun)(yun)解(jie)(jie)(jie)凍(dong)使(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。◎若非必(bi)(bi)要(yao)(yao)(yao)，無(wu)(wu)(wu)須(xu)進(jin)(jin)(jin)(jin)行熱(re)(re)滅(mie)(mie)活(huo)(huo)。若必(bi)(bi)須(xu)做血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)熱(re)(re)滅(mie)(mie)活(huo)(huo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，須(xu)嚴格(ge)控制(zhi)在(zai)56℃水(shui)浴(yu)30分(fen)(fen)(fen)(fen)(fen)鐘均勻(yun)(yun)(yun)(yun)加(jia)(jia)熱(re)(re)滅(mie)(mie)活(huo)(huo)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)定(ding)義血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)指(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)除去(qu)纖維(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)原(yuan)(yuan)后(hou)(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠狀液(ye)(ye)(ye)體(ti)(ti)。每100ml人血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)含有(you)(you)(you)(you)(you)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)(zhi)6-8g，其(qi)中(zhong)(zhong)(zhong)主(zhu)要(yao)(yao)(yao)是(shi)(shi)(shi)(shi)白(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)和(he)(he)(he)(he)(he)球(qiu)(qiu)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)不(bu)(bu)會(hui)凝固(gu)。有(you)(you)(you)(you)(you)免(mian)疫，維(wei)持(chi)酸堿平(ping)衡和(he)(he)(he)(he)(he)滲(shen)透壓(ya)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)(zhi)亦可(ke)(ke)(ke)(ke)(ke)(ke)儲備(bei)供給(gei)機(ji)體(ti)(ti)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)(zhi)不(bu)(bu)足(zu)時(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。人和(he)(he)(he)(he)(he)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)常(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)以(yi)進(jin)(jin)(jin)(jin)行各(ge)種(zhong)(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)學(xue)試(shi)(shi)驗(yan)，幫助(zhu)診斷(duan)(duan)疾病(bing)(bing)。含抗(kang)體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)(ke)(ke)(ke)(ke)作(zuo)為(wei)預防(fang)(fang)或(huo)(huo)治療疾病(bing)(bing)之(zhi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要(yao)(yao)(yao)成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)由血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)去(qu)除纖維(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)原(yuan)(yuan)而(er)(er)形成(cheng)(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)種(zhong)(zhong)(zhong)很復雜的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)物(wu)(wu)(wu)(wu)(wu)，其(qi)組(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)雖(sui)大(da)(da)(da)部(bu)(bu)分(fen)(fen)(fen)(fen)(fen)已知，但(dan)還有(you)(you)(you)(you)(you)一(yi)部(bu)(bu)分(fen)(fen)(fen)(fen)(fen)尚(shang)不(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)及含量(liang)(liang)(liang)(liang)(liang)常(chang)(chang)隨(sui)供血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)(xing)別、年齡(ling)、生理(li)(li)條件(jian)和(he)(he)(he)(he)(he)營養條件(jian)不(bu)(bu)同而(er)(er)異(yi)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)中(zhong)(zhong)(zhong)不(bu)(bu)含纖維(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)原(yuan)(yuan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠狀液(ye)(ye)(ye)體(ti)(ti),具(ju)有(you)(you)(you)(you)(you)維(wei)持(chi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正(zheng)常(chang)(chang)粘度(du)(du)、酸堿度(du)(du)、滲(shen)透壓(ya)等(deng)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。它主(zhu)要(yao)(yao)(yao)由水(shui)和(he)(he)(he)(he)(he)各(ge)種(zhong)(zhong)(zhong)化學(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)組(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)，這(zhe)(zhe)些化學(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)包括白(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)，α1、α2、β、γ-球(qiu)(qiu)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)，甘(gan)油三酯，總膽固(gu)醇，谷丙轉(zhuan)氨酶(mei)等(deng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)含有(you)(you)(you)(you)(you)各(ge)種(zhong)(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)、多(duo)(duo)(duo)(duo)肽(tai)、脂(zhi)肪、碳水(shui)化合(he)物(wu)(wu)(wu)(wu)(wu)、生長因(yin)(yin)子(zi)、激(ji)(ji)素(su)、無(wu)(wu)(wu)機(ji)物(wu)(wu)(wu)(wu)(wu)等(deng)，這(zhe)(zhe)些物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)對(dui)促(cu)進(jin)(jin)(jin)(jin)細(xi)(xi)(xi)(xi)胞(bao)(bao)生長或(huo)(huo)抑制(zhi)生長活(huo)(huo)性(xing)(xing)(xing)是(shi)(shi)(shi)(shi)達到生理(li)(li)平(ping)衡的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。對(dui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)和(he)(he)(he)(he)(he)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)研(yan)究(jiu)雖(sui)有(you)(you)(you)(you)(you)很大(da)(da)(da)進(jin)(jin)(jin)(jin)展，但(dan)仍(reng)存在(zai)一(yi)些問題。主(zhu)要(yao)(yao)(yao)是(shi)(shi)(shi)(shi)：第一(yi)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)可(ke)(ke)(ke)(ke)(ke)(ke)能(neng)(neng)有(you)(you)(you)(you)(you)幾(ji)百種(zhong)(zhong)(zhong)之(zhi)多(duo)(duo)(duo)(duo)，對(dui)其(qi)準確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)、含量(liang)(liang)(liang)(liang)(liang)及其(qi)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)機(ji)制(zhi)仍(reng)不(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，尤其(qi)是(shi)(shi)(shi)(shi)對(dui)其(qi)中(zhong)(zhong)(zhong)一(yi)些多(duo)(duo)(duo)(duo)肽(tai)類(lei)生長因(yin)(yin)子(zi)、激(ji)(ji)素(su)和(he)(he)(he)(he)(he)脂(zhi)類(lei)等(deng)尚(shang)未充分(fen)(fen)(fen)(fen)(fen)認識，這(zhe)(zhe)給(gei)研(yan)究(jiu)工作(zuo)帶(dai)來(lai)許多(duo)(duo)(duo)(duo)困(kun)難；第二：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都是(shi)(shi)(shi)(shi)批(pi)量(liang)(liang)(liang)(liang)(liang)生產(chan)，各(ge)批(pi)量(liang)(liang)(liang)(liang)(liang)之(zhi)間(jian)(jian)差異(yi)很大(da)(da)(da)，而(er)(er)且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)保(bao)存期至多(duo)(duo)(duo)(duo)一(yi)年，因(yin)(yin)此，要(yao)(yao)(yao)保(bao)證(zheng)(zheng)每批(pi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)相似性(xing)(xing)(xing)極為(wei)困(kun)難，從而(er)(er)使(shi)(shi)(shi)(shi)(shi)實(shi)驗(yan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標準化和(he)(he)(he)(he)(he)連續性(xing)(xing)(xing)受到限制(zhi)；第三：不(bu)(bu)能(neng)(neng)排除血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)含有(you)(you)(you)(you)(you)易變(bian)物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)，這(zhe)(zhe)被認為(wei)是(shi)(shi)(shi)(shi)“瓶中(zhong)(zhong)(zhong)惡(e)化”的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)(yuan)因(yin)(yin)之(zhi)一(yi)
大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)（去(qu)紅(hong)細(xi)(xi)胞(bao)、無(wu)(wu)菌過(guo)濾）血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)注(zhu)意事項(xiang)：防止(zhi)(zhi)發病(bing)免(mian)疫(yi)(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)多(duo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)馬、牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液制備，馬、牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)對(dui)其(qi)它動物(wu)(wu)(wu)來(lai)說(shuo)，也(ye)具(ju)(ju)有(you)(you)(you)(you)(you)抗(kang)原性(xing)，有(you)(you)(you)(you)(you)引起(qi)(qi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病(bing)的(de)(de)(de)(de)(de)(de)(de)(de)可(ke)(ke)(ke)(ke)能(neng)，因(yin)此(ci)，使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)免(mian)疫(yi)(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)要(yao)(yao)(yao)(yao)注(zhu)意防止(zhi)(zhi)引起(qi)(qi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病(bing)，預(yu)防的(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)要(yao)(yao)(yao)(yao)措(cuo)施是(shi)(shi)(shi)(shi)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)提純的(de)(de)(de)(de)(de)(de)(de)(de)制品，不(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)合格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)產(chan)品；另外，不(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)有(you)(you)(you)(you)(you)急功近利(li)的(de)(de)(de)(de)(de)(de)(de)(de)想法(fa)，要(yao)(yao)(yao)(yao)按照(zhao)要(yao)(yao)(yao)(yao)求劑量(liang)(liang)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，一(yi)次(ci)(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)量(liang)(liang)不(bu)(bu)(bu)(bu)可(ke)(ke)(ke)(ke)過(guo)大(da)。大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)取(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)操作(zuo)(zuo)(zuo)步(bu)驟：1.剪(jian)(jian)尾(wei)(wei)(wei)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue) 小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)每(mei)(mei)次(ci)(ci)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)0.1ml，大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)每(mei)(mei)次(ci)(ci)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)0.3-0.5ml左手(shou)(shou)(shou)(shou)(shou)拇指(zhi)(zhi)和(he)(he)(he)食(shi)指(zhi)(zhi)從背(bei)(bei)(bei)部(bu)(bu)(bu)(bu)(bu)(bu)捉(zhuo)住(zhu)(zhu)(zhu)鼠(shu)(shu)(shu)(shu)(shu)(shu)頸(jing)(jing)部(bu)(bu)(bu)(bu)(bu)(bu)皮(pi)膚(fu)(fu)，將(jiang)鼠(shu)(shu)(shu)(shu)(shu)(shu)頭(tou)朝下(xia)(xia)(xia)，鼠(shu)(shu)(shu)(shu)(shu)(shu)保(bao)定后將(jiang)其(qi)尾(wei)(wei)(wei)巴(ba)置于50℃熱(re)(re)水(shui)(shui)(shui)中(zhong)(zhong)(zhong)浸泡(pao)數(shu)分(fen)(fen)(fen)鐘，使(shi)(shi)(shi)尾(wei)(wei)(wei)部(bu)(bu)(bu)(bu)(bu)(bu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管(guan)(guan)(guan)充(chong)盈。擦干(gan)尾(wei)(wei)(wei)部(bu)(bu)(bu)(bu)(bu)(bu)，再用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)剪(jian)(jian)刀或(huo)刀片(pian)剪(jian)(jian)去(qu)尾(wei)(wei)(wei)尖1-2mm，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)管(guan)(guan)(guan)接流出(chu)的(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液，同(tong)時(shi)自尾(wei)(wei)(wei)根(gen)部(bu)(bu)(bu)(bu)(bu)(bu)向(xiang)尾(wei)(wei)(wei)尖按-摩。取(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)棉球(qiu)壓(ya)榨止(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)并用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)6%液體(ti)(ti)火棉膠(jiao)(jiao)涂在(zai)(zai)(zai)(zai)傷(shang)口處(chu)(chu)止(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。 2.去(qu)除(chu)(chu)(chu)(chu)眼(yan)(yan)(yan)(yan)球(qiu)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)0.5-1ml左手(shou)(shou)(shou)(shou)(shou)捉(zhuo)住(zhu)(zhu)(zhu)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)頸(jing)(jing)部(bu)(bu)(bu)(bu)(bu)(bu)皮(pi)膚(fu)(fu)，輕(qing)壓(ya)在(zai)(zai)(zai)(zai)實驗(yan)臺上，取(qu)(qu)側臥(wo)(wo)位(wei)(wei)，左手(shou)(shou)(shou)(shou)(shou)食(shi)指(zhi)(zhi)盡量(liang)(liang)將(jiang)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)眼(yan)(yan)(yan)(yan)周(zhou)皮(pi)膚(fu)(fu)往(wang)頸(jing)(jing)后壓(ya)，使(shi)(shi)(shi)眼(yan)(yan)(yan)(yan)球(qiu)杰出(chu)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)眼(yan)(yan)(yan)(yan)科彎鑷迅速夾去(qu)眼(yan)(yan)(yan)(yan)球(qiu)，將(jiang)鼠(shu)(shu)(shu)(shu)(shu)(shu)倒(dao)立，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)器(qi)皿接住(zhu)(zhu)(zhu)流出(chu)的(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液。采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)完(wan)(wan)(wan)(wan)(wan)畢立即(ji)(ji)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)紗(sha)布壓(ya)榨止(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)少用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。 3.心(xin)臟采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)0.5-0.6ml，大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)1-1.5ml鼠(shu)(shu)(shu)(shu)(shu)(shu)仰(yang)臥(wo)(wo)位(wei)(wei)固(gu)定，剪(jian)(jian)去(qu)胸(xiong)前區被毛(mao)，皮(pi)膚(fu)(fu)消毒后，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)左手(shou)(shou)(shou)(shou)(shou)食(shi)指(zhi)(zhi)在(zai)(zai)(zai)(zai)左側第3-4肋間(jian)觸摸到心(xin)搏(bo)處(chu)(chu)，右(you)手(shou)(shou)(shou)(shou)(shou)持(chi)帶(dai)有(you)(you)(you)(you)(you)4-5號針頭(tou)的(de)(de)(de)(de)(de)(de)(de)(de)注(zhu)射器(qi)，選擇心(xin)搏(bo)zui強處(chu)(chu)穿刺(ci)，當(dang)刺(ci)中(zhong)(zhong)(zhong)心(xin)臟時(shi)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液會(hui)主(zhu)(zhu)動進(jin)(jin)入(ru)注(zhu)射器(qi)。 4.斷頭(tou)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.8-1.2ml，大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)5-10ml左手(shou)(shou)(shou)(shou)(shou)拇指(zhi)(zhi)和(he)(he)(he)食(shi)指(zhi)(zhi)從背(bei)(bei)(bei)部(bu)(bu)(bu)(bu)(bu)(bu)捉(zhuo)住(zhu)(zhu)(zhu)鼠(shu)(shu)(shu)(shu)(shu)(shu)頸(jing)(jing)部(bu)(bu)(bu)(bu)(bu)(bu)皮(pi)膚(fu)(fu)，將(jiang)鼠(shu)(shu)(shu)(shu)(shu)(shu)頭(tou)朝下(xia)(xia)(xia)，右(you)手(shou)(shou)(shou)(shou)(shou)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)剪(jian)(jian)刀剪(jian)(jian)斷鼠(shu)(shu)(shu)(shu)(shu)(shu)頸(jing)(jing)部(bu)(bu)(bu)(bu)(bu)(bu)約1/2-4/5，讓血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液流入(ru)試(shi)管(guan)(guan)(guan)。 5.眼(yan)(yan)(yan)(yan)眶靜(jing)脈(mo)(mo)叢采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)為(wei)(wei)(wei)(wei)0.2-0.4ml，大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)為(wei)(wei)(wei)(wei)0.4-0.8ml取(qu)(qu)內徑為(wei)(wei)(wei)(wei)1.0-1.5mm的(de)(de)(de)(de)(de)(de)(de)(de)玻(bo)璃毛(mao)細(xi)(xi)管(guan)(guan)(guan)，臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前折斷成(cheng)(cheng)(cheng)(cheng)1～1.5cm長(chang)的(de)(de)(de)(de)(de)(de)(de)(de)毛(mao)細(xi)(xi)管(guan)(guan)(guan)段，浸入(ru)1％肝素溶(rong)(rong)液中(zhong)(zhong)(zhong)，干(gan)燥后用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。取(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)時(shi)左手(shou)(shou)(shou)(shou)(shou)捉(zhuo)住(zhu)(zhu)(zhu)鼠(shu)(shu)(shu)(shu)(shu)(shu)兩(liang)耳之(zhi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)頸(jing)(jing)背(bei)(bei)(bei)部(bu)(bu)(bu)(bu)(bu)(bu)皮(pi)膚(fu)(fu)以(yi)(yi)固(gu)定頭(tou)部(bu)(bu)(bu)(bu)(bu)(bu)，悄悄向(xiang)下(xia)(xia)(xia)壓(ya)榨頸(jing)(jing)部(bu)(bu)(bu)(bu)(bu)(bu)兩(liang)邊(bian)，引起(qi)(qi)頭(tou)部(bu)(bu)(bu)(bu)(bu)(bu)靜(jing)脈(mo)(mo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液回(hui)流困(kun)難(nan)使(shi)(shi)(shi)眼(yan)(yan)(yan)(yan)眶靜(jing)脈(mo)(mo)叢充(chong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)，右(you)手(shou)(shou)(shou)(shou)(shou)持(chi)毛(mao)細(xi)(xi)管(guan)(guan)(guan)由內眥部(bu)(bu)(bu)(bu)(bu)(bu)刺(ci)進(jin)(jin)結膜，再悄悄向(xiang)眼(yan)(yan)(yan)(yan)底(di)部(bu)(bu)(bu)(bu)(bu)(bu)方向(xiang)推進(jin)(jin)，悄悄旋轉毛(mao)細(xi)(xi)管(guan)(guan)(guan)以(yi)(yi)劃破靜(jing)脈(mo)(mo)叢，讓血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液順毛(mao)細(xi)(xi)管(guan)(guan)(guan)流出(chu)，接收入(ru)事前準備的(de)(de)(de)(de)(de)(de)(de)(de)容(rong)器(qi)中(zhong)(zhong)(zhong)。采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后紗(sha)布輕(qing)壓(ya)眼(yan)(yan)(yan)(yan)部(bu)(bu)(bu)(bu)(bu)(bu)止(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)、大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)、豚(tun)鼠(shu)(shu)(shu)(shu)(shu)(shu)及家兔均(jun)(jun)可(ke)(ke)(ke)(ke)采(cai)(cai)(cai)納此(ci)法(fa)取(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。刺(ci)入(ru)深(shen)度(du)(du)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)為(wei)(wei)(wei)(wei)2-3mm，可(ke)(ke)(ke)(ke)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.2-0.3ml；大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)為(wei)(wei)(wei)(wei)4-5mm，可(ke)(ke)(ke)(ke)采(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.4-0.8ml。大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)（去(qu)紅(hong)細(xi)(xi)胞(bao)、無(wu)(wu)菌過(guo)濾）Tips：1. 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)保(bao)存(cun)(cun)(cun)(cun)：在(zai)(zai)(zai)(zai)-15℃以(yi)(yi)下(xia)(xia)(xia)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)(you)(you)(you)效(xiao)期為(wei)(wei)(wei)(wei)5年，4℃可(ke)(ke)(ke)(ke)保(bao)存(cun)(cun)(cun)(cun)數(shu)周(zhou)，不(bu)(bu)(bu)(bu)宜(yi)室(shi)(shi)溫(wen)保(bao)存(cun)(cun)(cun)(cun)，不(bu)(bu)(bu)(bu)宜(yi)反復(fu)凍(dong)(dong)(dong)(dong)融(rong)。配制成(cheng)(cheng)(cheng)(cheng)完(wan)(wan)(wan)(wan)(wan)全(quan)培養(yang)(yang)基后應(ying)在(zai)(zai)(zai)(zai)四周(zhou)內用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)(wan)(wan)(wan)，一(yi)瓶(ping)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)如無(wu)(wu)法(fa)一(yi)次(ci)(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)(wan)(wan)(wan)應(ying)無(wu)(wu)菌分(fen)(fen)(fen)裝成(cheng)(cheng)(cheng)(cheng)恰當(dang)數(shu)量(liang)(liang)并冷凍(dong)(dong)(dong)(dong)保(bao)存(cun)(cun)(cun)(cun)。2、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)絮(xu)狀(zhuang)沉淀(dian)(dian)物(wu)(wu)(wu)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)多(duo)種蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)質(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)混(hun)合物(wu)(wu)(wu)，在(zai)(zai)(zai)(zai)凍(dong)(dong)(dong)(dong)融(rong)過(guo)程(cheng)中(zhong)(zhong)(zhong)會(hui)出(chu)現絮(xu)狀(zhuang)沉淀(dian)(dian)物(wu)(wu)(wu)，主(zhu)(zhu)要(yao)(yao)(yao)(yao)原因(yin)是(shi)(shi)(shi)(shi)由于血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)脂蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)和(he)(he)(he)纖(xian)(xian)維蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)變(bian)性(xing)所造(zao)成(cheng)(cheng)(cheng)(cheng)。但并不(bu)(bu)(bu)(bu)影(ying)(ying)響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本身(shen)的(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)量(liang)(liang)和(he)(he)(he)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。如需(xu)去(qu)除(chu)(chu)(chu)(chu)可(ke)(ke)(ke)(ke)先400g離心(xin)取(qu)(qu)上清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液配制成(cheng)(cheng)(cheng)(cheng)完(wan)(wan)(wan)(wan)(wan)全(quan)培養(yang)(yang)基再過(guo)濾即(ji)(ji)可(ke)(ke)(ke)(ke)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。3、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)解凍(dong)(dong)(dong)(dong)：合理(li)(li)的(de)(de)(de)(de)(de)(de)(de)(de)解凍(dong)(dong)(dong)(dong)方式(shi)可(ke)(ke)(ke)(ke)以(yi)(yi)更好的(de)(de)(de)(de)(de)(de)(de)(de)保(bao)護(hu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)量(liang)(liang)并可(ke)(ke)(ke)(ke)以(yi)(yi)在(zai)(zai)(zai)(zai)＊大(da)程(cheng)度(du)(du)上減少絮(xu)狀(zhuang)沉淀(dian)(dian)物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)產(chan)生(sheng)(sheng)(sheng)(sheng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從低溫(wen)冰箱中(zhong)(zhong)(zhong)取(qu)(qu)出(chu)后，經(jing)4℃冰箱、室(shi)(shi)溫(wen)、37℃水(shui)(shui)(shui)浴逐步(bu)規則搖晃均(jun)(jun)勻融(rong)解，直至(zhi)完(wan)(wan)(wan)(wan)(wan)全(quan)融(rong)解后再使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。4、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)熱(re)(re)滅(mie)(mie)活(huo)：將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)嚴格(ge)56℃水(shui)(shui)(shui)浴均(jun)(jun)勻加溫(wen)30分(fen)(fen)(fen)鐘可(ke)(ke)(ke)(ke)以(yi)(yi)滅(mie)(mie)活(huo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)補體(ti)(ti)系統。是(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)是(shi)(shi)(shi)(shi)所有(you)(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)途的(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都需(xu)要(yao)(yao)(yao)(yao)熱(re)(re)滅(mie)(mie)活(huo)呢？對(dui)大(da)多(duo)數(shu)細(xi)(xi)胞(bao)來(lai)說(shuo)是(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)需(xu)要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)。激(ji)活(huo)的(de)(de)(de)(de)(de)(de)(de)(de)補體(ti)(ti)能(neng)參與溶(rong)(rong)解細(xi)(xi)胞(bao)，刺(ci)激(ji)光(guang)滑肌收縮，細(xi)(xi)胞(bao)和(he)(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)板釋放(fang)組(zu)胺，激(ji)活(huo)淋巴(ba)細(xi)(xi)胞(bao)和(he)(he)(he)巨噬細(xi)(xi)胞(bao)。因(yin)此(ci)在(zai)(zai)(zai)(zai)免(mian)疫(yi)(yi)學(xue)(xue)研究，培養(yang)(yang)ES細(xi)(xi)胞(bao)，昆蟲細(xi)(xi)胞(bao)和(he)(he)(he)平(ping)滑肌肉細(xi)(xi)胞(bao)時(shi)，建議(yi)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)熱(re)(re)滅(mie)(mie)活(huo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，在(zai)(zai)(zai)(zai)正常(chang)情(qing)況(kuang)下(xia)(xia)(xia)加熱(re)(re)處(chu)(chu)理(li)(li)對(dui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)促(cu)細(xi)(xi)胞(bao)生(sheng)(sheng)(sheng)(sheng)長(chang)效(xiao)應(ying)無(wu)(wu)明(ming)顯促(cu)進(jin)(jin)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，甚(shen)至(zhi)會(hui)因(yin)為(wei)(wei)(wei)(wei)加熱(re)(re)處(chu)(chu)理(li)(li)影(ying)(ying)響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)量(liang)(liang)。5、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)“小(xiao)(xiao)黑點(dian)”現象：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)經(jing)過(guo)一(yi)段時(shi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)低溫(wen)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)(cun)再融(rong)解后，在(zai)(zai)(zai)(zai)正常(chang)情(qing)況(kuang)下(xia)(xia)(xia)會(hui)出(chu)現絮(xu)狀(zhuang)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)沉淀(dian)(dian)。但往(wang)往(wang)有(you)(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶反應(ying)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)有(you)(you)(you)(you)(you)“小(xiao)(xiao)黑點(dian)”現象，認(ren)(ren)為(wei)(wei)(wei)(wei)是(shi)(shi)(shi)(shi)微生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)污(wu)染。實驗(yan)發現血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)37℃環境放(fang)置時(shi)間(jian)過(guo)長(chang)或(huo)經(jing)過(guo)加熱(re)(re)滅(mie)(mie)活(huo)處(chu)(chu)理(li)(li)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)沉淀(dian)(dian)物(wu)(wu)(wu)往(wang)往(wang)會(hui)呈增(zeng)多(duo)趨勢，這(zhe)些沉淀(dian)(dian)物(wu)(wu)(wu)在(zai)(zai)(zai)(zai)倒(dao)置顯微鏡下(xia)(xia)(xia)觀(guan)察，像是(shi)(shi)(shi)(shi)“小(xiao)(xiao)黑點(dian)”，讓使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)者誤以(yi)(yi)為(wei)(wei)(wei)(wei)是(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)遭受微生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)污(wu)染并在(zai)(zai)(zai)(zai)分(fen)(fen)(fen)裂增(zeng)殖。因(yin)此(ci)如無(wu)(wu)必要(yao)(yao)(yao)(yao)，完(wan)(wan)(wan)(wan)(wan)全(quan)可(ke)(ke)(ke)(ke)以(yi)(yi)不(bu)(bu)(bu)(bu)進(jin)(jin)行熱(re)(re)滅(mie)(mie)活(huo)處(chu)(chu)理(li)(li)并保(bao)證血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)低溫(wen)保(bao)存(cun)(cun)(cun)(cun)。實驗(yan)證明(ming)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)注(zhu)意以(yi)(yi)下(xia)(xia)(xia)幾點(dian)事項(xiang)不(bu)(bu)(bu)(bu)但可(ke)(ke)(ke)(ke)以(yi)(yi)保(bao)證血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)量(liang)(liang)并能(neng)給使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)者帶(dai)來(lai)方便：◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)(ke)(ke)在(zai)(zai)(zai)(zai)-15℃以(yi)(yi)下(xia)(xia)(xia)低溫(wen)保(bao)存(cun)(cun)(cun)(cun)。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)瓶(ping)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)次(ci)(ci)無(wu)(wu)法(fa)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)(wan)(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)應(ying)分(fen)(fen)(fen)裝凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)(cun)，分(fen)(fen)(fen)次(ci)(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)(wan)(wan)(wan)。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)按-15℃以(yi)(yi)下(xia)(xia)(xia)至(zhi)4℃至(zhi)室(shi)(shi)溫(wen)至(zhi)37℃逐步(bu)均(jun)(jun)勻解凍(dong)(dong)(dong)(dong)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。◎若非必要(yao)(yao)(yao)(yao)，無(wu)(wu)須進(jin)(jin)行熱(re)(re)滅(mie)(mie)活(huo)。若必須做血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)熱(re)(re)滅(mie)(mie)活(huo)的(de)(de)(de)(de)(de)(de)(de)(de)，須嚴格(ge)控制在(zai)(zai)(zai)(zai)56℃水(shui)(shui)(shui)浴30分(fen)(fen)(fen)鐘均(jun)(jun)勻加熱(re)(re)滅(mie)(mie)活(huo)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)定義血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)指(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)除(chu)(chu)(chu)(chu)去(qu)纖(xian)(xian)維蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)原后的(de)(de)(de)(de)(de)(de)(de)(de)膠(jiao)(jiao)狀(zhuang)液體(ti)(ti)。每(mei)(mei)100ml人血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)含(han)(han)有(you)(you)(you)(you)(you)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)質(zhi)6-8g，其(qi)中(zhong)(zhong)(zhong)主(zhu)(zhu)要(yao)(yao)(yao)(yao)是(shi)(shi)(shi)(shi)白(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)和(he)(he)(he)球(qiu)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)不(bu)(bu)(bu)(bu)會(hui)凝(ning)固(gu)。有(you)(you)(you)(you)(you)免(mian)疫(yi)(yi)，維持(chi)酸堿平(ping)衡(heng)和(he)(he)(he)滲透(tou)壓(ya)的(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)質(zhi)亦可(ke)(ke)(ke)(ke)儲備供給機體(ti)(ti)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)質(zhi)不(bu)(bu)(bu)(bu)足時(shi)的(de)(de)(de)(de)(de)(de)(de)(de)應(ying)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。人和(he)(he)(he)動物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)常(chang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)以(yi)(yi)進(jin)(jin)行各(ge)(ge)種血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)學(xue)(xue)試(shi)驗(yan)，幫助診斷疾病(bing)。含(han)(han)抗(kang)體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)(ke)(ke)作(zuo)(zuo)(zuo)為(wei)(wei)(wei)(wei)預(yu)防或(huo)治療疾病(bing)之(zhi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)要(yao)(yao)(yao)(yao)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)由血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)去(qu)除(chu)(chu)(chu)(chu)纖(xian)(xian)維蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)原而(er)形成(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)一(yi)種很(hen)(hen)復(fu)雜的(de)(de)(de)(de)(de)(de)(de)(de)混(hun)合物(wu)(wu)(wu)，其(qi)組(zu)成(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)份雖大(da)部(bu)(bu)(bu)(bu)(bu)(bu)分(fen)(fen)(fen)已(yi)知，但還(huan)有(you)(you)(you)(you)(you)一(yi)部(bu)(bu)(bu)(bu)(bu)(bu)分(fen)(fen)(fen)尚不(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚(chu)，且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組(zu)成(cheng)(cheng)(cheng)(cheng)及含(han)(han)量(liang)(liang)常(chang)隨(sui)供血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)性(xing)別、年齡、生(sheng)(sheng)(sheng)(sheng)理(li)(li)條件和(he)(he)(he)營(ying)養(yang)(yang)條件不(bu)(bu)(bu)(bu)同(tong)而(er)異。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)中(zhong)(zhong)(zhong)不(bu)(bu)(bu)(bu)含(han)(han)纖(xian)(xian)維蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)原的(de)(de)(de)(de)(de)(de)(de)(de)膠(jiao)(jiao)狀(zhuang)液體(ti)(ti),具(ju)(ju)有(you)(you)(you)(you)(you)維持(chi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液的(de)(de)(de)(de)(de)(de)(de)(de)正常(chang)粘度(du)(du)、酸堿度(du)(du)、滲透(tou)壓(ya)等作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。它主(zhu)(zhu)要(yao)(yao)(yao)(yao)由水(shui)(shui)(shui)和(he)(he)(he)各(ge)(ge)種化(hua)學(xue)(xue)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)組(zu)成(cheng)(cheng)(cheng)(cheng)，這(zhe)些化(hua)學(xue)(xue)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)包括白(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)，α1、α2、β、γ-球(qiu)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)，甘油三酯，總膽固(gu)醇，谷丙轉氨酶等。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)含(han)(han)有(you)(you)(you)(you)(you)各(ge)(ge)種血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)、多(duo)肽、脂肪、碳水(shui)(shui)(shui)化(hua)合物(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)子、激(ji)素、無(wu)(wu)機物(wu)(wu)(wu)等，這(zhe)些物(wu)(wu)(wu)質(zhi)對(dui)促(cu)進(jin)(jin)細(xi)(xi)胞(bao)生(sheng)(sheng)(sheng)(sheng)長(chang)或(huo)抑制生(sheng)(sheng)(sheng)(sheng)長(chang)活(huo)性(xing)是(shi)(shi)(shi)(shi)達到生(sheng)(sheng)(sheng)(sheng)理(li)(li)平(ping)衡(heng)的(de)(de)(de)(de)(de)(de)(de)(de)。對(dui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)和(he)(he)(he)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)研究雖有(you)(you)(you)(you)(you)很(hen)(hen)大(da)進(jin)(jin)展，但仍存(cun)(cun)(cun)(cun)在(zai)(zai)(zai)(zai)一(yi)些問題。主(zhu)(zhu)要(yao)(yao)(yao)(yao)是(shi)(shi)(shi)(shi)：第一(yi)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)份可(ke)(ke)(ke)(ke)能(neng)有(you)(you)(you)(you)(you)幾百種之(zhi)多(duo)，對(dui)其(qi)準確的(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)份、含(han)(han)量(liang)(liang)及其(qi)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)機制仍不(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚(chu)，尤其(qi)是(shi)(shi)(shi)(shi)對(dui)其(qi)中(zhong)(zhong)(zhong)一(yi)些多(duo)肽類生(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)子、激(ji)素和(he)(he)(he)脂類等尚未充(chong)分(fen)(fen)(fen)認(ren)(ren)識，這(zhe)給研究工作(zuo)(zuo)(zuo)帶(dai)來(lai)許多(duo)困(kun)難(nan)；第二(er)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都是(shi)(shi)(shi)(shi)批量(liang)(liang)生(sheng)(sheng)(sheng)(sheng)產(chan)，各(ge)(ge)批量(liang)(liang)之(zhi)間(jian)差(cha)異很(hen)(hen)大(da)，而(er)且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)保(bao)存(cun)(cun)(cun)(cun)期至(zhi)多(duo)一(yi)年，因(yin)此(ci)，要(yao)(yao)(yao)(yao)保(bao)證每(mei)(mei)批血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)相似性(xing)極為(wei)(wei)(wei)(wei)困(kun)難(nan)，從而(er)使(shi)(shi)(shi)實驗(yan)的(de)(de)(de)(de)(de)(de)(de)(de)標準化(hua)和(he)(he)(he)連續性(xing)受到限制；第三：不(bu)(bu)(bu)(bu)能(neng)排(pai)除(chu)(chu)(chu)(chu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)含(han)(han)有(you)(you)(you)(you)(you)易變(bian)物(wu)(wu)(wu)質(zhi)，這(zhe)被認(ren)(ren)為(wei)(wei)(wei)(wei)是(shi)(shi)(shi)(shi)“瓶(ping)中(zhong)(zhong)(zhong)惡化(hua)”的(de)(de)(de)(de)(de)(de)(de)(de)原因(yin)之(zhi)一(yi)
雞(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)（去(qu)紅細(xi)(xi)胞(bao)(bao)(bao)(bao)、無(wu)(wu)菌(jun)過(guo)濾(lv)）適用(yong)(yong)(yong)(yong)(yong)范(fan)圍【產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)名(ming)稱】 雞(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（無(wu)(wu)菌(jun)過(guo)濾(lv)）【產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)規格】100ml/200ml/500ml/500ml*20瓶【產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)價(jia)格】130元(yuan)-4500元(yuan)【適用(yong)(yong)(yong)(yong)(yong)范(fan)圍]適應于(yu)(yu)(yu)(yu)(yu)細(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)、免疫(yi)(yi)檢測等(deng)(deng)(deng)。【儲存(cun)條件及(ji)有(you)(you)(you)效期]1、密封(feng)保存(cun)于(yu)(yu)(yu)(yu)(yu)-10℃~-30℃2、有(you)(you)(you)效期五年[注(zhu)意(yi)事(shi)項]1、本產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)僅供(gong)科研(yan)使(shi)用(yong)(yong)(yong)(yong)(yong)。2、產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)使(shi)用(yong)(yong)(yong)(yong)(yong)過(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)，注(zhu)意(yi)防止(zhi)污染，在室溫內(nei)放置(zhi)過(guo)久(jiu)會影(ying)響使(shi)用(yong)(yong)(yong)(yong)(yong)效果，使(shi)用(yong)(yong)(yong)(yong)(yong)后請盡快放入(ru)(ru)-10℃~-33、請勿(wu)將使(shi)用(yong)(yong)(yong)(yong)(yong)后剩余的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試劑(ji)再倒入(ru)(ru)原(yuan)(yuan)包裝(zhuang)瓶內(nei)。【生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)日期]詳見(jian)標(biao)簽，請在產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)標(biao)簽標(biao)注(zhu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)(you)效期內(nei)使(shi)用(yong)(yong)(yong)(yong)(yong)。產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)介紹 雞(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)細(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)天(tian)然(ran)(ran)培(pei)養(yang)(yang)(yang)基,含有(you)(you)(you)豐富的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長(chang)必(bi)(bi)須的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)營(ying)養(yang)(yang)(yang)成(cheng)(cheng)(cheng)份(fen),常用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)動(dong)物(wu)細(xi)(xi)胞(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)體(ti)(ti)(ti)(ti)外(wai)培(pei)養(yang)(yang)(yang),具(ju)(ju)(ju)有(you)(you)(you)極(ji)為重(zhong)要的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)功能。1.提(ti)供(gong)對(dui)維持細(xi)(xi)胞(bao)(bao)(bao)(bao)指數生(sheng)(sheng)(sheng)(sheng)長(chang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)激素,基礎培(pei)養(yang)(yang)(yang)基中(zhong)(zhong)(zhong)(zhong)沒有(you)(you)(you)或(huo)(huo)量(liang)很(hen)少(shao)(shao)(shao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)營(ying)養(yang)(yang)(yang)物(wu),以及(ji)主(zhu)要的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低(di)分(fen)(fen)(fen)子(zi)營(ying)養(yang)(yang)(yang)物(wu)。2. 提(ti)供(gong)結(jie)(jie)合蛋(dan)(dan)白(bai)(bai),能識(shi)別維生(sheng)(sheng)(sheng)(sheng)素、脂類(lei)、金屬(shu)和其(qi)他激素等(deng)(deng)(deng),能結(jie)(jie)合或(huo)(huo)調變(bian)它(ta)(ta)們所結(jie)(jie)合的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)質(zhi)(zhi)(zhi)活力。3.有(you)(you)(you)些情況(kuang)下(xia)結(jie)(jie)合蛋(dan)(dan)白(bai)(bai)質(zhi)(zhi)(zhi)能與有(you)(you)(you)毒金屬(shu)和熱(re)原(yuan)(yuan)質(zhi)(zhi)(zhi)結(jie)(jie)合,起到作(zuo)用(yong)(yong)(yong)(yong)(yong)。4.是(shi)(shi)(shi)(shi)細(xi)(xi)胞(bao)(bao)(bao)(bao)貼壁、鋪展在塑料培(pei)養(yang)(yang)(yang)基質(zhi)(zhi)(zhi)上所需因(yin)(yin)(yin)子(zi)來源。5.起酸堿度緩沖(chong)液作(zuo)用(yong)(yong)(yong)(yong)(yong)。 6.提(ti)供(gong)蛋(dan)(dan)白(bai)(bai)酶(mei)抑制(zhi)劑(ji),使(shi)在細(xi)(xi)胞(bao)(bao)(bao)(bao)傳代(dai)時(shi)使(shi)剩余胰蛋(dan)(dan)白(bai)(bai)酶(mei)失活,保護細(xi)(xi)胞(bao)(bao)(bao)(bao)不(bu)(bu)(bu)受傷害。 雞(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)分(fen)(fen)(fen)和作(zuo)用(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)研(yan)究雖有(you)(you)(you)很(hen)大進(jin)(jin)展,但仍(reng)存(cun)在一(yi)(yi)(yi)些問題(ti)。主(zhu)要是(shi)(shi)(shi)(shi):第(di)一(yi)(yi)(yi):血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)份(fen)可能有(you)(you)(you)幾百種(zhong)之多(duo),目前對(dui)其(qi)準(zhun)(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)份(fen)、含量(liang)及(ji)其(qi)作(zuo)用(yong)(yong)(yong)(yong)(yong)機制(zhi)仍(reng)不(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚,尤其(qi)是(shi)(shi)(shi)(shi)對(dui)其(qi)中(zhong)(zhong)(zhong)(zhong)一(yi)(yi)(yi)些多(duo)肽類(lei)生(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)、激素和脂類(lei)等(deng)(deng)(deng)尚(shang)未充分(fen)(fen)(fen)認識(shi),這給(gei)研(yan)究工作(zuo)帶來許(xu)多(duo)困難;第(di)二:血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都是(shi)(shi)(shi)(shi)批(pi)(pi)(pi)量(liang)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan),各批(pi)(pi)(pi)量(liang)之間(jian)差異很(hen)大,而且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)保存(cun)期至多(duo)一(yi)(yi)(yi)年,因(yin)(yin)(yin)此,要保證每批(pi)(pi)(pi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)相似性極(ji)為困難,從而使(shi)實驗的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)準(zhun)(zhun)化和連續(xu)性受到限制(zhi);第(di)三:不(bu)(bu)(bu)能排除血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)含有(you)(you)(you)易(yi)變(bian)物(wu)質(zhi)(zhi)(zhi),這被認為是(shi)(shi)(shi)(shi)“瓶中(zhong)(zhong)(zhong)(zhong)”的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)(yuan)因(yin)(yin)(yin)之一(yi)(yi)(yi)。 雞(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)瓶裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)凍需采用(yong)(yong)(yong)(yong)(yong)逐步解(jie)(jie)(jie)凍法(fa)(fa):-20℃ 至 -70℃ 低(di)溫冰(bing)箱中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)放入(ru)(ru)4℃冰(bing)箱中(zhong)(zhong)(zhong)(zhong)溶(rong)解(jie)(jie)(jie)1天(tian).然(ran)(ran)后移入(ru)(ru)室溫,待全(quan)部溶(rong)解(jie)(jie)(jie)后再分(fen)(fen)(fen)裝(zhuang).在溶(rong)解(jie)(jie)(jie)過(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)需不(bu)(bu)(bu)斷輕(qing)輕(qing)搖晃均(jun)勻(小心勿(wu)造(zao)成(cheng)(cheng)(cheng)氣泡(pao)),使(shi)溫度與成(cheng)(cheng)(cheng)分(fen)(fen)(fen)均(jun)一(yi)(yi)(yi),減(jian)(jian)少(shao)(shao)(shao)沉(chen)淀(dian)(dian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)生(sheng)(sheng)(sheng)(sheng).切勿(wu)直(zhi)(zhi)接(jie)將血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從-20℃進(jin)(jin)入(ru)(ru)37℃解(jie)(jie)(jie)凍,這樣因(yin)(yin)(yin)溫度改(gai)變(bian)太(tai)大,容(rong)易(yi)造(zao)成(cheng)(cheng)(cheng)蛋(dan)(dan)白(bai)(bai)質(zhi)(zhi)(zhi)凝集(ji)而出現沉(chen)淀(dian)(dian).雞(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)（去(qu)紅細(xi)(xi)胞(bao)(bao)(bao)(bao)、無(wu)(wu)菌(jun)過(guo)濾(lv)）雞(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)其(qi)它(ta)(ta)產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)特點:雞(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)嬌貴細(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)、細(xi)(xi)胞(bao)(bao)(bao)(bao)株保存(cun)特級(ji)新生(sheng)(sheng)(sheng)(sheng)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)雜(za)交、骨(gu)質(zhi)(zhi)(zhi)、原(yuan)(yuan)代(dai)、傳代(dai)細(xi)(xi)胞(bao)(bao)(bao)(bao)等(deng)(deng)(deng)細(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)及(ji)疫(yi)(yi)苗(miao)、診(zhen)(zhen)(zhen)斷試劑(ji)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)等(deng)(deng)(deng)馬血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科研(yan)、診(zhen)(zhen)(zhen)斷試劑(ji)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)等(deng)(deng)(deng)山羊血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科研(yan)、診(zhen)(zhen)(zhen)斷試劑(ji)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)等(deng)(deng)(deng)兔(tu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科研(yan)等(deng)(deng)(deng)驢血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科研(yan)、診(zhen)(zhen)(zhen)斷試劑(ji)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)等(deng)(deng)(deng)豬血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科研(yan)、診(zhen)(zhen)(zhen)斷試劑(ji)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)等(deng)(deng)(deng)雞(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科研(yan)、診(zhen)(zhen)(zhen)斷試劑(ji)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)等(deng)(deng)(deng)無(wu)(wu)菌(jun)脫(tuo)纖維綿羊血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科研(yan)、診(zhen)(zhen)(zhen)斷試劑(ji)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)等(deng)(deng)(deng)無(wu)(wu)菌(jun)脫(tuo)纖維兔(tu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科研(yan)、診(zhen)(zhen)(zhen)斷試劑(ji)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)等(deng)(deng)(deng)羊血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科研(yan)等(deng)(deng)(deng)兔(tu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科研(yan)等(deng)(deng)(deng)產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)應用(yong)(yong)(yong)(yong)(yong)： 用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)雜(za)交瘤細(xi)(xi)胞(bao)(bao)(bao)(bao)、骨(gu)質(zhi)(zhi)(zhi)瘤細(xi)(xi)胞(bao)(bao)(bao)(bao)、原(yuan)(yuan)代(dai)、傳代(dai)細(xi)(xi)胞(bao)(bao)(bao)(bao)等(deng)(deng)(deng)細(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)及(ji)疫(yi)(yi)苗(miao)、診(zhen)(zhen)(zhen)斷試劑(ji)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)；雞(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)IgG的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)純化及(ji)其(qi)單(dan)(dan)抗制(zhi)備(bei)與鑒定(ding)： IgG是(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)主(zhu)要的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)免疫(yi)(yi)球蛋(dan)(dan)白(bai)(bai)(Ig),占血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)Ig總量(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)75%~80%,它(ta)(ta)是(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液中(zhong)(zhong)(zhong)(zhong)主(zhu)要的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)抗體(ti)(ti)(ti)(ti)成(cheng)(cheng)(cheng)分(fen)(fen)(fen),血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)IgG水平的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測定(ding),已(yi)是(shi)(shi)(shi)(shi)疫(yi)(yi)苗(miao)免疫(yi)(yi)效果評定(ding)和體(ti)(ti)(ti)(ti)液免疫(yi)(yi)研(yan)究的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)常用(yong)(yong)(yong)(yong)(yong)指標(biao)。與常規免疫(yi)(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)相比,單(dan)(dan)克隆(long)抗體(ti)(ti)(ti)(ti)具(ju)(ju)(ju)有(you)(you)(you)與抗原(yuan)(yuan)結(jie)(jie)合的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)高特異性,是(shi)(shi)(shi)(shi)免疫(yi)(yi)學和血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)學研(yan)究的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)重(zhong)要工具(ju)(ju)(ju),在畜(chu)牧(mu)獸醫的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)疾(ji)病診(zhen)(zhen)(zhen)斷與治(zhi)療方面(mian)已(yi)經被廣泛應用(yong)(yong)(yong)(yong)(yong)。在養(yang)(yang)(yang)禽業中(zhong)(zhong)(zhong)(zhong),運用(yong)(yong)(yong)(yong)(yong)這些單(dan)(dan)克隆(long)抗體(ti)(ti)(ti)(ti)來檢測疫(yi)(yi)病特異性的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)抗體(ti)(ti)(ti)(ti),對(dui)全(quan)面(mian)、系(xi)統地(di)了(le)解(jie)(jie)(jie)禽的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)機體(ti)(ti)(ti)(ti)對(dui)病原(yuan)(yuan)體(ti)(ti)(ti)(ti)感(gan)染的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)免疫(yi)(yi)應答反應并采取相應的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)防制(zhi)措(cuo)施有(you)(you)(you)重(zhong)要意(yi)義。雞(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)背景(jing) 雞(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)細(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)用(yong)(yong)(yong)(yong)(yong)量(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)天(tian)然(ran)(ran)培(pei)養(yang)(yang)(yang)基，含有(you)(you)(you)豐富的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長(chang)必(bi)(bi)須的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)營(ying)養(yang)(yang)(yang)成(cheng)(cheng)(cheng)份(fen)，常用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)動(dong)物(wu)細(xi)(xi)胞(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)體(ti)(ti)(ti)(ti)外(wai)培(pei)養(yang)(yang)(yang)，具(ju)(ju)(ju)有(you)(you)(you)極(ji)為重(zhong)要的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)功能。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必(bi)(bi)須貯(zhu)存(cun)于(yu)(yu)(yu)(yu)(yu)–20～-70 oC，若(ruo)存(cun)放于(yu)(yu)(yu)(yu)(yu)4度，不(bu)(bu)(bu)能超(chao)過(guo)一(yi)(yi)(yi)個(ge)月。如果一(yi)(yi)(yi)次無(wu)(wu)法(fa)(fa)用(yong)(yong)(yong)(yong)(yong)完一(yi)(yi)(yi)瓶，可將40～45 ml分(fen)(fen)(fen)裝(zhuang)于(yu)(yu)(yu)(yu)(yu)無(wu)(wu)菌(jun)50 ml離心管中(zhong)(zhong)(zhong)(zhong)，由(you)(you)于(yu)(yu)(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結(jie)(jie)凍時(shi)體(ti)(ti)(ti)(ti)積(ji)會增加(jia)約10%，必(bi)(bi)須預留10%膨脹體(ti)(ti)(ti)(ti)積(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)空間(jian)，否則(ze)易(yi)發(fa)生(sheng)(sheng)(sheng)(sheng)污染或(huo)(huo)容(rong)器凍裂。 瓶裝(zhuang)(500ml)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)凍步驟(逐步解(jie)(jie)(jie)凍法(fa)(fa)):從-20 oC或(huo)(huo)–70 oC取出放至4 oC冰(bing)箱溶(rong)解(jie)(jie)(jie)一(yi)(yi)(yi)天(tian)，再置(zhi)于(yu)(yu)(yu)(yu)(yu)室溫下(xia)全(quan)溶(rong)后分(fen)(fen)(fen)裝(zhuang)，一(yi)(yi)(yi)般50 ml無(wu)(wu)菌(jun)離心管可分(fen)(fen)(fen)裝(zhuang)40～45 ml。在溶(rong)解(jie)(jie)(jie)過(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)須規則(ze)搖晃均(jun)勻(小心勿(wu)造(zao)成(cheng)(cheng)(cheng)氣泡(pao))，使(shi)溫度與成(cheng)(cheng)(cheng)分(fen)(fen)(fen)均(jun)一(yi)(yi)(yi)，減(jian)(jian)少(shao)(shao)(shao)沈淀(dian)(dian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)生(sheng)(sheng)(sheng)(sheng)。切勿(wu)直(zhi)(zhi)接(jie)由(you)(you)–20 oC直(zhi)(zhi)接(jie)至37 oC解(jie)(jie)(jie)凍，因(yin)(yin)(yin)溫度改(gai)變(bian)太(tai)大，容(rong)易(yi)造(zao)成(cheng)(cheng)(cheng)蛋(dan)(dan)白(bai)(bai)質(zhi)(zhi)(zhi)凝結(jie)(jie)而發(fa)生(sheng)(sheng)(sheng)(sheng)沈淀(dian)(dian)。勿(wu)將血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)置(zhi)于(yu)(yu)(yu)(yu)(yu)37 oC太(tai)久(jiu)，若(ruo)在37 oC放置(zhi)太(tai)久(jiu)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)會變(bian)得混濁，同時(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)許(xu)多(duo)不(bu)(bu)(bu)穩定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)份(fen)也會因(yin)(yin)(yin)此受到破壞，從而影(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)品(pin)(pin)(pin)(pin)質(zhi)(zhi)(zhi)。
馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)（去紅細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、無(wu)(wu)菌(jun)(jun)過(guo)濾）產(chan)品介紹 采(cai)用(yong)(yong)健康動(dong)物(wu)(wu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)為(wei)原(yuan)料，經(jing)(jing)(jing)無(wu)(wu)菌(jun)(jun)采(cai)集，分(fen)(fen)離(li)(li)，微(wei)(wei)孔過(guo)濾后(hou)而成(cheng)(cheng)(cheng)(cheng)，性(xing)狀(zhuang)為(wei)橙清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)，無(wu)(wu)噬菌(jun)(jun)體(ti)(ti)(ti)(ti)(ti)(ti)，無(wu)(wu)溶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)，無(wu)(wu)異物(wu)(wu)，無(wu)(wu)細(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)，真菌(jun)(jun)支(zhi)(zhi)原(yuan)體(ti)(ti)(ti)(ti)(ti)(ti)毒(du)形(xing)體(ti)(ti)(ti)(ti)(ti)(ti)衣原(yuan)體(ti)(ti)(ti)(ti)(ti)(ti)等，適(shi)應(ying)于(yu)(yu)(yu)(yu)試驗室或生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化科(ke)研相(xiang)(xiang)關試驗，滿足不(bu)(bu)同(tong)科(ke)研實(shi)驗的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)多(duo)種(zhong)需求注意事項(xiang)1、注意避免反(fan)復凍融。反(fan)復凍融可(ke)能(neng)(neng)產(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)沉淀，可(ke)以(yi)(yi)(yi)通(tong)過(guo)無(wu)(wu)菌(jun)(jun)條件下，3500 rp m離(li)(li)心10 min除(chu)去沉淀。2、注意無(wu)(wu)菌(jun)(jun)操作(zuo)(zuo)。標簽(qian)：馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)說(shuo)明(ming)(ming)(ming)書 ；馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)規格產(chan)品用(yong)(yong)途1、可(ke)用(yong)(yong)于(yu)(yu)(yu)(yu)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)或誘(you)導樹突細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)，且細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長，增(zeng)殖(zhi)狀(zhuang)況良好 有(you)(you)研究人員分(fen)(fen)別(bie)(bie)(bie)用(yong)(yong)胎(tai)(tai)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)和(he)(he)(he)(he)馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)馬(ma)(ma)(ma)單(dan)核(he)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)來產(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)權(quan)突狀(zhuang)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)（eqMoDC），結(jie)果發(fa)(fa)(fa)現，馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)eqMoDC與(yu)FBS生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)eqMoDC沒有(you)(you)區別(bie)(bie)(bie)。然而，與(yu)馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)補充培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)基(ji)(ji)(ji)一(yi)(yi)起(qi)孵(fu)育(yu)(yu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)e qMo DC在(zai)(zai)(zai)(zai)從單(dan)核(he)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)分(fen)(fen)化過(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)表現出更具特(te)(te)(te)(te)征性(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樹突狀(zhuang)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)形(xing)態。在(zai)(zai)(zai)(zai)用(yong)(yong)馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)eqMoDC中(zhong)(zhong)(zhong)(zhong)(zhong)也(ye)(ye)觀察到(dao)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)活力的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)顯著增(zeng)加(jia)(jia)。此外(wai)，發(fa)(fa)(fa)現在(zai)(zai)(zai)(zai)馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)存(cun)(cun)在(zai)(zai)(zai)(zai)下產(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)eqMoDC在(zai)(zai)(zai)(zai)功能(neng)(neng)性(xing)T淋巴(ba)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)啟動(dong)能(neng)(neng)力方(fang)面優(you)越，并(bing)且引(yin)發(fa)(fa)(fa)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)非特(te)(te)(te)(te)異性(xing)增(zeng)殖(zhi)顯著減(jian)(jian)少。2、可(ke)用(yong)(yong)于(yu)(yu)(yu)(yu)原(yuan)代培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)神經(jing)(jing)(jing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao) 有(you)(you)人用(yong)(yong)含(han)10%馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)DMEM，分(fen)(fen)離(li)(li)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)BALB/c小(xiao)鼠原(yuan)代腦神經(jing)(jing)(jing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)。發(fa)(fa)(fa)現和(he)(he)(he)(he)添(tian)加(jia)(jia)2%神經(jing)(jing)(jing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長劑(ji)B27的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Neurobasal培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)基(ji)(ji)(ji)相(xiang)(xiang)比(bi)，含(han)10%馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)DMEM培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)基(ji)(ji)(ji)更節省成(cheng)(cheng)(cheng)(cheng)本，方(fang)便廉價。3、特(te)(te)(te)(te)定(ding)脂(zhi)(zhi)(zhi)肪(fang)酸產(chan)物(wu)(wu)濃度(du)(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),對造血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)有(you)(you)支(zhi)(zhi)持(chi)作(zuo)(zuo)用(yong)(yong) 研究表明(ming)(ming)(ming)，磷脂(zhi)(zhi)(zhi)酶(mei)A2特(te)(te)(te)(te)定(ding)脂(zhi)(zhi)(zhi)肪(fang)酸產(chan)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃度(du)(du)馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，支(zhi)(zhi)持(chi)多(duo)能(neng)(neng)小(xiao)鼠造血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)祖細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)FDCP-Mix細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)自我更新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)能(neng)(neng)力。4、可(ke)增(zeng)強豬單(dan)性(xing)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)殖(zhi)胚(pei)(pei)(pei)(pei)胎(tai)(tai)植入前(qian)，胚(pei)(pei)(pei)(pei)層的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)(fa)(fa)育(yu)(yu)。 有(you)(you)研究通(tong)過(guo)對比(bi)不(bu)(bu)同(tong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)和(he)(he)(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)樣物(wu)(wu)質(zhi)(zhi)，對豬單(dan)性(xing)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)殖(zhi)胚(pei)(pei)(pei)(pei)胎(tai)(tai)植入前(qian)發(fa)(fa)(fa)育(yu)(yu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)影響，尋找適(shi)合其(qi)發(fa)(fa)(fa)育(yu)(yu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)基(ji)(ji)(ji)。對單(dan)性(xing)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)殖(zhi)胚(pei)(pei)(pei)(pei)胎(tai)(tai)進行(xing)豬濾泡液(ye)(ye)(ye)(ye)(ye)（PFF)、胎(tai)(tai)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(（FBS)、馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（HS）或豬血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)（PSA)處理，培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)2天后(hou)。通(tong)過(guo)囊(nang)胚(pei)(pei)(pei)(pei)形(xing)成(cheng)(cheng)(cheng)(cheng)和(he)(he)(he)(he)孵(fu)化，發(fa)(fa)(fa)現馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)增(zeng)強胚(pei)(pei)(pei)(pei)層發(fa)(fa)(fa)育(yu)(yu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)＊有(you)(you)效蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)來源。接下來，使(shi)用(yong)(yong)三種(zhong)不(bu)(bu)同(tong)濃度(du)(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)HS (10%，20%和(he)(he)(he)(he)30%）來確定(ding)改善(shan)豬單(dan)性(xing)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)殖(zhi)胚(pei)(pei)(pei)(pei)胎(tai)(tai)發(fa)(fa)(fa)育(yu)(yu)所(suo)需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)適(shi)宜HS濃度(du)(du)。所(suo)有(you)(you)HS濃度(du)(du)均增(zeng)加(jia)(jia)了(le)囊(nang)胚(pei)(pei)(pei)(pei)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)數(shu)量(liang)，降低了(le)囊(nang)胚(pei)(pei)(pei)(pei)凋亡細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)率，其(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)20%效果更顯著。5、馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)添(tian)加(jia)(jia)到(dao)微(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)基(ji)(ji)(ji)中(zhong)(zhong)(zhong)(zhong)(zhong)，可(ke)用(yong)(yong)于(yu)(yu)(yu)(yu)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)微(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)，如(ru)(ru)∶(1）馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)添(tian)加(jia)(jia)到(dao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)基(ji)(ji)(ji)中(zhong)(zhong)(zhong)(zhong)(zhong)用(yong)(yong)于(yu)(yu)(yu)(yu)支(zhi)(zhi)原(yuan)體(ti)(ti)(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)。在(zai)(zai)(zai)(zai)《獸藥典》中(zhong)(zhong)(zhong)(zhong)(zhong)，含(han)馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)支(zhi)(zhi)原(yuan)體(ti)(ti)(ti)(ti)(ti)(ti)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)基(ji)(ji)(ji)被(bei)用(yong)(yong)于(yu)(yu)(yu)(yu)獸用(yong)(yong)疫苗的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)支(zhi)(zhi)原(yuan)體(ti)(ti)(ti)(ti)(ti)(ti)檢查。用(yong)(yong)KM2培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)基(ji)(ji)(ji)(含(han)馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing))還可(ke)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)豬肺(fei)炎支(zhi)(zhi)原(yuan)體(ti)(ti)(ti)(ti)(ti)(ti)。(2）馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)加(jia)(jia)入到(dao)固體(ti)(ti)(ti)(ti)(ti)(ti)和(he)(he)(he)(he)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)基(ji)(ji)(ji)中(zhong)(zhong)(zhong)(zhong)(zhong)，可(ke)用(yong)(yong)于(yu)(yu)(yu)(yu)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)幽門螺桿(gan)菌(jun)(jun)。并(bing)且，和(he)(he)(he)(he)羊(yang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)和(he)(he)(he)(he)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)比(bi)較，添(tian)加(jia)(jia)馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)基(ji)(ji)(ji)更易于(yu)(yu)(yu)(yu)分(fen)(fen)裝和(he)(he)(he)(he)保(bao)存(cun)(cun)。馬(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)（去紅細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、無(wu)(wu)菌(jun)(jun)過(guo)濾）檢驗報告：檢測項(xiang)目(mu)檢測結(jie)果外(wai)觀淡(dan)黃(huang)色液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)PH7.26無(wu)(wu)菌(jun)(jun)檢驗陰性(xing)總蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)68.6g/L白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)35.1g/L支(zhi)(zhi)原(yuan)體(ti)(ti)(ti)(ti)(ti)(ti)陰性(xing)內毒(du)素(su)(su)8UE/mL血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)紅蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)0.04g/L血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)(yao)作(zuo)(zuo)用(yong)(yong)●提供基(ji)(ji)(ji)本營養(yang)物(wu)(wu)質(zhi)(zhi)：氨基(ji)(ji)(ji)酸、維生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素(su)(su)、無(wu)(wu)機(ji)(ji)物(wu)(wu)、脂(zhi)(zhi)(zhi)類物(wu)(wu)質(zhi)(zhi)、核(he)酸衍生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)等，是(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長必須(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)質(zhi)(zhi)。●提供激素(su)(su)和(he)(he)(he)(he)各種(zhong)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長因(yin)(yin)子(zi)(zi)：胰(yi)島(dao)素(su)(su)、腎(shen)上腺皮質(zhi)(zhi)激素(su)(su)（氫化可(ke)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)松、地塞米(mi)松）、類固醇激素(su)(su)（雌二(er)(er)醇、睪酮、孕酮）等。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長因(yin)(yin)子(zi)(zi)如(ru)(ru)成(cheng)(cheng)(cheng)(cheng)纖維細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長因(yin)(yin)子(zi)(zi)、表皮生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長因(yin)(yin)子(zi)(zi)、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)板(ban)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長因(yin)(yin)子(zi)(zi)等。●提供結(jie)合蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)：結(jie)合蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)作(zuo)(zuo)用(yong)(yong)是(shi)(shi)攜(xie)帶(dai)(dai)重要(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低分(fen)(fen)子(zi)(zi)量(liang)物(wu)(wu)質(zhi)(zhi)，如(ru)(ru)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜(xie)帶(dai)(dai)維生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素(su)(su)、脂(zhi)(zhi)(zhi)肪(fang)、以(yi)(yi)(yi)及激素(su)(su)等，轉(zhuan)鐵蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜(xie)帶(dai)(dai)鐵。結(jie)合蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)在(zai)(zai)(zai)(zai)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)代謝過(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)起(qi)重要(yao)(yao)作(zuo)(zuo)用(yong)(yong)。●提供促接觸(chu)和(he)(he)(he)(he)伸展因(yin)(yin)子(zi)(zi)使(shi)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)(tie)壁(bi)免受機(ji)(ji)械損傷。●對培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)起(qi)到(dao)某(mou)些(xie)保(bao)護(hu)作(zuo)(zuo)用(yong)(yong)：有(you)(you)一(yi)(yi)些(xie)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)，如(ru)(ru)內皮細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、骨髓(sui)樣細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)可(ke)以(yi)(yi)(yi)釋放蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)含(han)有(you)(you)抗(kang)蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)，起(qi)到(dao)中(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)作(zuo)(zuo)用(yong)(yong)。這(zhe)種(zhong)作(zuo)(zuo)用(yong)(yong)是(shi)(shi)偶然發(fa)(fa)(fa)現的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，則(ze)有(you)(you)目(mu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)使(shi)用(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)來終止(zhi)胰(yi)蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消化作(zuo)(zuo)用(yong)(yong)。因(yin)(yin)為(wei)胰(yi)蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)已經(jing)(jing)(jing)被(bei)廣泛用(yong)(yong)于(yu)(yu)(yu)(yu)貼(tie)(tie)壁(bi)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消化傳代。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)形(xing)成(cheng)(cheng)(cheng)(cheng)了(le)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)粘(zhan)度(du)(du)，可(ke)以(yi)(yi)(yi)保(bao)護(hu)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)免受機(ji)(ji)械損傷，特(te)(te)(te)(te)別(bie)(bie)(bie)是(shi)(shi)在(zai)(zai)(zai)(zai)懸浮培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)攪拌時，粘(zhan)度(du)(du)起(qi)到(dao)重要(yao)(yao)作(zuo)(zuo)用(yong)(yong)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)還含(han)有(you)(you)一(yi)(yi)些(xie)微(wei)(wei)量(liang)元素(su)(su)和(he)(he)(he)(he)離(li)(li)子(zi)(zi)，他們在(zai)(zai)(zai)(zai)代謝解毒(du)中(zhong)(zhong)(zhong)(zhong)(zhong)起(qi)重要(yao)(yao)作(zuo)(zuo)用(yong)(yong)，如(ru)(ru)SeO3，硒(xi)等。鑒別(bie)(bie)(bie)方(fang)法 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)凝(ning)(ning)固析(xi)出的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)淡(dan)黃(huang)色透明(ming)(ming)(ming)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti) 。如(ru)(ru)將血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)自血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管內抽(chou)出，放入試管中(zhong)(zhong)(zhong)(zhong)(zhong)，不(bu)(bu)加(jia)(jia)抗(kang)凝(ning)(ning)劑(ji)，則(ze)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)應(ying)被(bei)激活，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)迅速凝(ning)(ning)固，形(xing)成(cheng)(cheng)(cheng)(cheng)膠凍。凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)塊收(shou)縮，其(qi)周圍所(suo)析(xi)出之(zhi)淡(dan)黃(huang)色透明(ming)(ming)(ming)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)即為(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，也(ye)(ye)可(ke)于(yu)(yu)(yu)(yu)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后(hou)經(jing)(jing)(jing)離(li)(li)心取得。在(zai)(zai)(zai)(zai)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)過(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)，纖維蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)轉(zhuan)變成(cheng)(cheng)(cheng)(cheng)纖維蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)塊，所(suo)以(yi)(yi)(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)無(wu)(wu)纖維蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)，這(zhe)一(yi)(yi)點(dian)是(shi)(shi)與(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)＊大(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie)(bie)(bie)。而在(zai)(zai)(zai)(zai)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)應(ying)中(zhong)(zhong)(zhong)(zhong)(zhong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)板(ban)釋放出許多(duo)物(wu)(wu)質(zhi)(zhi)，各凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)因(yin)(yin)子(zi)(zi)也(ye)(ye)都(dou)發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)了(le)變化。這(zhe)些(xie)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)都(dou)留在(zai)(zai)(zai)(zai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)并(bing)繼續發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)變化，如(ru)(ru)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)酶(mei)原(yuan)變成(cheng)(cheng)(cheng)(cheng)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)酶(mei)，并(bing)隨血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)存(cun)(cun)放時間逐漸減(jian)(jian)少以(yi)(yi)(yi)至消失(shi)。這(zhe)些(xie)也(ye)(ye)都(dou)是(shi)(shi)與(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)區別(bie)(bie)(bie)之(zhi)處。但大(da)(da)量(liang)未參(can)加(jia)(jia)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)應(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)質(zhi)(zhi)則(ze)與(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)基(ji)(ji)(ji)本相(xiang)(xiang)同(tong)。為(wei)避免抗(kang)凝(ning)(ning)劑(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)干(gan)擾，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)中(zhong)(zhong)(zhong)(zhong)(zhong)許多(duo)化學成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)析(xi)，都(dou)以(yi)(yi)(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)樣品。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)懸濁于(yu)(yu)(yu)(yu)其(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)。人體(ti)(ti)(ti)(ti)(ti)(ti)含(han)有(you)(you)2750-3300毫升血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)，約(yue)占血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)總體(ti)(ti)(ti)(ti)(ti)(ti)積的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)55%。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)絕(jue)大(da)(da)部分(fen)(fen)是(shi)(shi)水(shui)（體(ti)(ti)(ti)(ti)(ti)(ti)積的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)90%），其(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)溶解的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)質(zhi)(zhi)主要(yao)(yao)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)蛋(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)，還包括(kuo)葡萄糖(tang)、無(wu)(wu)機(ji)(ji)鹽離(li)(li)子(zi)(zi)、激素(su)(su)以(yi)(yi)(yi)及二(er)(er)氧(yang)化碳。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)(yao)功能(neng)(neng)是(shi)(shi)運載血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)，同(tong)時也(ye)(ye)是(shi)(shi)運輸分(fen)(fen)泌產(chan)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)(yao)媒介。
兔血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)（去(qu)紅細(xi)(xi)(xi)(xi)胞(bao)、無菌(jun)(jun)過(guo)濾(lv)）產(chan)(chan)品描述血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)采集(ji)自(zi)(zi)正(zheng)常(chang)健(jian)(jian)康(kang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)供(gong)體(ti)(ti)(ti)，經脂質(zhi)(zhi)萃取和(he)透析(xi)后儲存(cun)(cun)于(yu)緩沖液(ye)（0.01 M磷酸鈉(na)，0.25 M氯化鈉(na), pH 7.6）中(zhong)(zhong)(zhong)(zhong)(zhong)。常(chang)用(yong)于(yu)各(ge)(ge)種免疫實驗(yan)(yan)，起到(dao)(dao)封(feng)閉(bi)或對(dui)照的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)。本(ben)品用(yong)于(yu)封(feng)閉(bi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)常(chang)見(jian)使(shi)(shi)用(yong)濃(nong)度(du)(du)為(wei)(wei)(wei)(wei)5%（v/v）。產(chan)(chan)品使(shi)(shi)用(yong)1）血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)以(yi)凍(dong)干(gan)粉(fen)形式提供(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，本(ben)品需(xu)要(yao)(yao)(yao)先(xian)溶(rong)解(jie)(jie)(jie)(jie)配制成(cheng)(cheng)100%原液(ye)后再(zai)稀釋(shi)使(shi)(shi)用(yong)。溶(rong)解(jie)(jie)(jie)(jie)方(fang)(fang)法(fa)(fa)(fa)(fa)：用(yong)10ml 蒸餾水（dH2O）充分(fen)(fen)溶(rong)解(jie)(jie)(jie)(jie)凍(dong)干(gan)粉(fen)（如(ru)果不(bu)澄清(qing)(qing)(qing)(qing)(qing)(qing)(qing)，可(ke)(ke)(ke)對(dui)其(qi)離心(xin)(xin)(xin)處理），此時(shi)得到(dao)(dao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)即(ji)是(shi)(shi)100%原液(ye)。然后按(an)照1:20的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例(li)用(yong)緩沖液(ye)，如(ru)PBS或PBST，稀釋(shi)到(dao)(dao)需(xu)要(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)5%工(gong)(gong)作(zuo)(zuo)液(ye)。也(ye)(ye)(ye)可(ke)(ke)(ke)以(yi)根據自(zi)(zi)身(shen)實驗(yan)(yan)條(tiao)件(jian)，稀釋(shi)到(dao)(dao)合適的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)工(gong)(gong)作(zuo)(zuo)液(ye)濃(nong)度(du)(du)。2）血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)以(yi)100%原液(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)形式提供(gong)，直接用(yong)合適的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)緩沖液(ye)稀釋(shi)到(dao)(dao)需(xu)要(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)工(gong)(gong)作(zuo)(zuo)液(ye)。產(chan)(chan)品性質(zhi)(zhi)蛋(dan)(dan)白濃(nong)度(du)(du)（Protein concentration）60 mg/mL緩沖液(ye)（Buffer）0.01 M磷酸鈉(na)，0.25 M氯化鈉(na), pH 7.6防(fang)腐劑（Preservative）0.05%疊氮化鈉(na)運(yun)輸與(yu)(yu)保(bao)存(cun)(cun)方(fang)(fang)法(fa)(fa)(fa)(fa)冰袋運(yun)輸。凍(dong)干(gan)粉(fen)保(bao)存(cun)(cun)于(yu)2～8℃。100%原液(ye)保(bao)存(cun)(cun)于(yu)2～8℃，6個(ge)星期(qi)穩定。建(jian)(jian)(jian)議原液(ye)分(fen)(fen)裝(zhuang)后置于(yu)≤-20℃，以(yi)延長保(bao)存(cun)(cun)期(qi)。重溶(rong)后100%原液(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有效期(qi)1年。注(zhu)意(yi)事項1）本(ben)品含(han)疊氮化鈉(na)，對(dui)人(ren)體(ti)(ti)(ti)有害，請(qing)(qing)注(zhu)意(yi)適當防(fang)護。2）為(wei)(wei)(wei)(wei)了(le)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)安全和(he)健(jian)(jian)康(kang)，請(qing)(qing)穿實驗(yan)(yan)服(fu)并(bing)(bing)戴一(yi)(yi)次性手套操作(zuo)(zuo)。3）注(zhu)意(yi)無菌(jun)(jun)操作(zuo)(zuo)4）注(zhu)意(yi)避免反(fan)(fan)復凍(dong)融(rong)。反(fan)(fan)復凍(dong)融(rong)可(ke)(ke)(ke)能(neng)產(chan)(chan)生沉(chen)(chen)(chen)淀，可(ke)(ke)(ke)以(yi)通(tong)過(guo)無菌(jun)(jun)條(tiao)件(jian)下(xia)(xia)，3500rpm離心(xin)(xin)(xin)10min除去(qu)沉(chen)(chen)(chen)淀兔血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)（去(qu)紅細(xi)(xi)(xi)(xi)胞(bao)、無菌(jun)(jun)過(guo)濾(lv)）常(chang)見(jian)問題 保(bao)存(cun)(cun)方(fang)(fang)法(fa)(fa)(fa)(fa)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)應(ying)(ying)保(bao)存(cun)(cun)在(zai)(zai)-5℃至-20℃。然而(er)，若(ruo)(ruo)(ruo)存(cun)(cun)放(fang)(fang)(fang)于(yu)4℃時(shi)，請(qing)(qing)勿超過(guo)一(yi)(yi)個(ge)月。若(ruo)(ruo)(ruo)您一(yi)(yi)次無法(fa)(fa)(fa)(fa)用(yong)完一(yi)(yi)瓶，建(jian)(jian)(jian)議將(jiang)(jiang)無菌(jun)(jun)分(fen)(fen)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)至恰當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)滅菌(jun)(jun)容器內，再(zai)放(fang)(fang)(fang)回(hui)冷凍(dong)。解(jie)(jie)(jie)(jie)凍(dong)方(fang)(fang)法(fa)(fa)(fa)(fa)將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)從冷凍(dong)箱取出(chu)(chu)后，先(xian)置于(yu)2-8℃冰箱使(shi)(shi)之(zhi)溶(rong)解(jie)(jie)(jie)(jie)，然后在(zai)(zai)室溫下(xia)(xia)使(shi)(shi)之(zhi)全溶(rong)。但(dan)(dan)必須注(zhu)意(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)，溶(rong)解(jie)(jie)(jie)(jie)過(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)必須規則地搖晃均(jun)勻(yun)。避免產(chan)(chan)生沉(chen)(chen)(chen)淀物(wu)(wu)1、解(jie)(jie)(jie)(jie)凍(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)，請(qing)(qing)按(an)照所建(jian)(jian)(jian)議的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)逐步解(jie)(jie)(jie)(jie)凍(dong)法(fa)(fa)(fa)(fa)（-2℃至4℃至室溫），若(ruo)(ruo)(ruo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)凍(dong)時(shi)改(gai)變(bian)(bian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)溫度(du)(du)太大(da)(da)（如(ru)-20℃至37℃），實驗(yan)(yan)顯示非常(chang)容易產(chan)(chan)生沉(chen)(chen)(chen)淀物(wu)(wu)。2、解(jie)(jie)(jie)(jie)凍(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)，請(qing)(qing)隨時(shi)將(jiang)(jiang)之(zhi)搖晃均(jun)勻(yun)，使(shi)(shi)溫度(du)(du)及成(cheng)(cheng)分(fen)(fen)均(jun)一(yi)(yi)，減(jian)少沉(chen)(chen)(chen)淀的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發生。3、請(qing)(qing)勿將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)置于(yu)37℃太久。若(ruo)(ruo)(ruo)在(zai)(zai)37℃放(fang)(fang)(fang)置太久，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)會變(bian)(bian)得混濁，同時(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)許(xu)多較不(bu)穩定的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)分(fen)(fen)也(ye)(ye)(ye)會因(yin)此受到(dao)(dao)損害，而(er)影響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)量。4、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱(re)滅活(huo)非常(chang)容易造成(cheng)(cheng)沉(chen)(chen)(chen)淀物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)增多，若(ruo)(ruo)(ruo)非必要(yao)(yao)(yao)，可(ke)(ke)(ke)以(yi)無須做(zuo)此步驟(zou)。 [3]5、若(ruo)(ruo)(ruo)必須做(zuo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱(re)滅活(huo)，請(qing)(qing)遵守56℃，30分(fen)(fen)鐘的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原則，并(bing)(bing)且隨時(shi)搖晃均(jun)勻(yun)。溫度(du)(du)過(guo)高，時(shi)間過(guo)久或搖晃不(bu)均(jun)勻(yun)，都(dou)會造成(cheng)(cheng)沉(chen)(chen)(chen)淀物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)增多。若(ruo)(ruo)(ruo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)凍(dong)后發現(xian)有絮狀沉(chen)(chen)(chen)淀物(wu)(wu)出(chu)(chu)現(xian)，可(ke)(ke)(ke)以(yi)將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)分(fen)(fen)裝(zhuang)至無菌(jun)(jun)離心(xin)(xin)(xin)管內，以(yi)400g稍微離心(xin)(xin)(xin)，上清(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)即(ji)可(ke)(ke)(ke)接著加(jia)(jia)入培養基(ji)內一(yi)(yi)起過(guo)濾(lv)。但(dan)(dan)不(bu)建(jian)(jian)(jian)議以(yi)過(guo)濾(lv)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)法(fa)(fa)(fa)(fa)去(qu)除這些(xie)絮狀沉(chen)(chen)(chen)淀物(wu)(wu)，因(yin)為(wei)(wei)(wei)(wei)它可(ke)(ke)(ke)能(neng)會阻塞過(guo)濾(lv)膜。熱(re)滅活(huo)一(yi)(yi)般是(shi)(shi)以(yi)56℃，30分(fen)(fen)鐘來(lai)處理已解(jie)(jie)(jie)(jie)凍(dong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)，因(yin)為(wei)(wei)(wei)(wei)此加(jia)(jia)熱(re)步驟(zou)，可(ke)(ke)(ke)以(yi)使(shi)(shi)補(bu)體(ti)(ti)(ti)去(qu)活(huo)化，而(er)補(bu)體(ti)(ti)(ti)所參(can)與(yu)(yu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)反(fan)(fan)應(ying)(ying)有：溶(rong)細(xi)(xi)(xi)(xi)胞(bao)活(huo)性，平滑肌的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)收縮，肥大(da)(da)細(xi)(xi)(xi)(xi)胞(bao)和(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)板組胺的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)釋(shi)放(fang)(fang)(fang)，增強吞噬作(zuo)(zuo)用(yong)，淋(lin)巴細(xi)(xi)(xi)(xi)胞(bao)、巨噬細(xi)(xi)(xi)(xi)胞(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)趨化和(he)激(ji)活(huo)。鑒(jian)別(bie)(bie)方(fang)(fang)法(fa)(fa)(fa)(fa) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)凝(ning)(ning)(ning)(ning)固析(xi)出(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)淡黃(huang)色(se)透明液(ye)體(ti)(ti)(ti) 。如(ru)將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)自(zi)(zi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管內抽出(chu)(chu)，放(fang)(fang)(fang)入試管中(zhong)(zhong)(zhong)(zhong)(zhong)，不(bu)加(jia)(jia)抗(kang)凝(ning)(ning)(ning)(ning)劑，則凝(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)(fan)應(ying)(ying)被激(ji)活(huo)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)迅速凝(ning)(ning)(ning)(ning)固，形成(cheng)(cheng)膠凍(dong)。凝(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)塊(kuai)收縮，其(qi)周(zhou)圍所析(xi)出(chu)(chu)之(zhi)淡黃(huang)色(se)透明液(ye)體(ti)(ti)(ti)即(ji)為(wei)(wei)(wei)(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)，也(ye)(ye)(ye)可(ke)(ke)(ke)于(yu)凝(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后經離心(xin)(xin)(xin)取得。在(zai)(zai)凝(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)過(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)，纖維蛋(dan)(dan)白原轉變(bian)(bian)成(cheng)(cheng)纖維蛋(dan)(dan)白塊(kuai)，所以(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)無纖維蛋(dan)(dan)白原，這一(yi)(yi)點(dian)是(shi)(shi)與(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)＊大(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie)(bie)。而(er)在(zai)(zai)凝(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)(fan)應(ying)(ying)中(zhong)(zhong)(zhong)(zhong)(zhong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)板釋(shi)放(fang)(fang)(fang)出(chu)(chu)許(xu)多物(wu)(wu)質(zhi)(zhi)，各(ge)(ge)凝(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)因(yin)子也(ye)(ye)(ye)都(dou)發生了(le)變(bian)(bian)化。這些(xie)成(cheng)(cheng)分(fen)(fen)都(dou)留在(zai)(zai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)并(bing)(bing)繼續發生變(bian)(bian)化，如(ru)凝(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)酶(mei)原變(bian)(bian)成(cheng)(cheng)凝(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)酶(mei)，并(bing)(bing)隨血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)存(cun)(cun)放(fang)(fang)(fang)時(shi)間逐漸減(jian)少以(yi)至消失(shi)。這些(xie)也(ye)(ye)(ye)都(dou)是(shi)(shi)與(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)區別(bie)(bie)之(zhi)處。但(dan)(dan)大(da)(da)量未參(can)加(jia)(jia)凝(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)(fan)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)質(zhi)(zhi)則與(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)基(ji)本(ben)相(xiang)同。為(wei)(wei)(wei)(wei)避免抗(kang)凝(ning)(ning)(ning)(ning)劑的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)干(gan)擾(rao)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)中(zhong)(zhong)(zhong)(zhong)(zhong)許(xu)多化學成(cheng)(cheng)分(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)析(xi)，都(dou)以(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)(wei)(wei)(wei)樣品。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)體(ti)(ti)(ti)成(cheng)(cheng)分(fen)(fen)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)(xi)(xi)胞(bao)懸濁于(yu)其(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)。人(ren)體(ti)(ti)(ti)含(han)有2750-3300毫升(sheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)，約占血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)總體(ti)(ti)(ti)積(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)55%。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)絕大(da)(da)部分(fen)(fen)是(shi)(shi)水（體(ti)(ti)(ti)積(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)90%），其(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)溶(rong)解(jie)(jie)(jie)(jie)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)質(zhi)(zhi)主(zhu)要(yao)(yao)(yao)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)蛋(dan)(dan)白，還包(bao)括葡萄(tao)糖、無機鹽離子、激(ji)素以(yi)及二氧化碳(tan)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要(yao)(yao)(yao)功能(neng)是(shi)(shi)運(yun)載血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)(xi)(xi)胞(bao)，同時(shi)也(ye)(ye)(ye)是(shi)(shi)運(yun)輸分(fen)(fen)泌產(chan)(chan)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要(yao)(yao)(yao)媒介。將(jiang)(jiang)新(xin)鮮血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)離心(xin)(xin)(xin)，使(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)(xi)(xi)胞(bao)沉(chen)(chen)(chen)降，上層淡黃(huang)色(se)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)即(ji)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)與(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie)(bie)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)不(bu)含(han)纖維蛋(dan)(dan)白原等(deng)凝(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)因(yin)子。
胎(tai)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿（去(qu)(qu)紅細(xi)(xi)胞(bao)(bao)(bao)、無(wu)(wu)(wu)菌(jun)過(guo)濾）產(chan)(chan)(chan)(chan)品(pin)規格(ge)(ge)：100ml/200ml/500ml保(bao)(bao)(bao) 存(cun)：-20℃，三(san)年。優(you)級新生(sheng)(sheng)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（無(wu)(wu)(wu)菌(jun)過(guo)濾） 使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)時(shi)常(chang)見問題及(ji)解(jie)(jie)(jie)(jie)決方(fang)法(fa)(fa)：一(yi)(yi)(yi)(yi)、保(bao)(bao)(bao)存(cun)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)方(fang)法(fa)(fa)?血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)應保(bao)(bao)(bao)存(cun)在(zai)(zai)(zai)-5℃至(zhi)(zhi)(zhi)-2O℃若(ruo)(ruo)存(cun)放于4℃時(shi)，請(qing)(qing)勿超過(guo)一(yi)(yi)(yi)(yi)個(ge)月。若(ruo)(ruo)您一(yi)(yi)(yi)(yi)次無(wu)(wu)(wu)法(fa)(fa)用(yong)(yong)(yong)(yong)(yong)完(wan)(wan)(wan)一(yi)(yi)(yi)(yi)瓶，建議(yi)將(jiang)無(wu)(wu)(wu)菌(jun)分(fen)(fen)(fen)(fen)裝血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)至(zhi)(zhi)(zhi)恰(qia)當的(de)(de)(de)(de)(de)(de)(de)(de)滅(mie)菌(jun)容(rong)(rong)器內，再放回(hui)冷(leng)凍(dong)(dong)。二、如(ru)何解(jie)(jie)(jie)(jie)凍(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)才不(bu)(bu)會(hui)(hui)使(shi)(shi)產(chan)(chan)(chan)(chan)品(pin)質量(liang)(liang)(liang)受損?將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從冷(leng)凍(dong)(dong)箱(xiang)取出(chu)后，先置于2-8℃冰箱(xiang)使(shi)(shi)之(zhi)溶(rong)解(jie)(jie)(jie)(jie)，然(ran)后在(zai)(zai)(zai)室(shi)(shi)溫(wen)(wen)下(xia)使(shi)(shi)之(zhi)全(quan)溶(rong)。但(dan)必須(xu)(xu)注(zhu)(zhu)意(yi)的(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)，溶(rong)解(jie)(jie)(jie)(jie)過(guo)程(cheng)中(zhong)(zhong)(zhong)必須(xu)(xu)規則地搖(yao)晃均(jun)(jun)(jun)勻(yun)(yun)(yun)(yun)(yun)。三(san)、如(ru)何避(bi)免(mian)(mian)沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)產(chan)(chan)(chan)(chan)生(sheng)(sheng)?1.解(jie)(jie)(jie)(jie)凍(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)，請(qing)(qing)按照(zhao)所(suo)(suo)建議(yi)的(de)(de)(de)(de)(de)(de)(de)(de)逐(zhu)步(bu)解(jie)(jie)(jie)(jie)凍(dong)(dong)法(fa)(fa)(-2O℃至(zhi)(zhi)(zhi)4℃至(zhi)(zhi)(zhi)室(shi)(shi)溫(wen)(wen))，若(ruo)(ruo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)凍(dong)(dong)時(shi)改變(bian)的(de)(de)(de)(de)(de)(de)(de)(de)溫(wen)(wen)度(du)(du)太大(如(ru)-20℃至(zhi)(zhi)(zhi)37℃)，實驗(yan)顯示非常(chang)容(rong)(rong)易(yi)產(chan)(chan)(chan)(chan)生(sheng)(sheng)沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)。2.解(jie)(jie)(jie)(jie)凍(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)，請(qing)(qing)隨時(shi)將(jiang)之(zhi)搖(yao)晃均(jun)(jun)(jun)勻(yun)(yun)(yun)(yun)(yun)，使(shi)(shi)溫(wen)(wen)度(du)(du)及(ji)成(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)均(jun)(jun)(jun)一(yi)(yi)(yi)(yi)，減少沉(chen)淀(dian)(dian)(dian)的(de)(de)(de)(de)(de)(de)(de)(de)發(fa)生(sheng)(sheng)3.請(qing)(qing)勿將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)置于37℃太久(jiu)。若(ruo)(ruo)在(zai)(zai)(zai)37℃放置太久(jiu)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)會(hui)(hui)變(bian)得(de)混(hun)(hun)濁，同時(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)許多(duo)(duo)較不(bu)(bu)穩定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)也會(hui)(hui)因(yin)(yin)(yin)此(ci)受到損害，而(er)(er)影響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)質量(liang)(liang)(liang)。4.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)熱(re)(re)(re)(re)滅(mie)活(huo)(huo)(huo)(huo)非常(chang)容(rong)(rong)易(yi)造成(cheng)(cheng)(cheng)沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)增多(duo)(duo)，若(ruo)(ruo)非必要(yao)(yao)，可(ke)(ke)以(yi)(yi)(yi)(yi)(yi)無(wu)(wu)(wu)須(xu)(xu)做(zuo)此(ci)步(bu)驟。5.若(ruo)(ruo)必須(xu)(xu)做(zuo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)熱(re)(re)(re)(re)滅(mie)活(huo)(huo)(huo)(huo)，請(qing)(qing)遵守56℃，30分(fen)(fen)(fen)(fen)鐘的(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)(yuan)則，并(bing)且隨時(shi)搖(yao)晃均(jun)(jun)(jun)勻(yun)(yun)(yun)(yun)(yun)。溫(wen)(wen)度(du)(du)過(guo)高，時(shi)間(jian)過(guo)久(jiu)或(huo)搖(yao)晃不(bu)(bu)均(jun)(jun)(jun)勻(yun)(yun)(yun)(yun)(yun)，都會(hui)(hui)造成(cheng)(cheng)(cheng)沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)增多(duo)(duo)。四、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)凍(dong)(dong)后發(fa)現(xian)(xian)有(you)(you)(you)(you)絮(xu)(xu)(xu)狀(zhuang)(zhuang)沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)出(chu)現(xian)(xian)，該如(ru)何處(chu)(chu)理(li)? 欲去(qu)(qu)除這些(xie)絮(xu)(xu)(xu)狀(zhuang)(zhuang)沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)，可(ke)(ke)以(yi)(yi)(yi)(yi)(yi)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)分(fen)(fen)(fen)(fen)裝至(zhi)(zhi)(zhi)無(wu)(wu)(wu)菌(jun)離心(xin)(xin)管內，以(yi)(yi)(yi)(yi)(yi)400g稍微(wei)離心(xin)(xin)，上清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)即可(ke)(ke)接著(zhu)加入培(pei)養(yang)(yang)基內一(yi)(yi)(yi)(yi)起過(guo)濾。但(dan)不(bu)(bu)建議(yi)以(yi)(yi)(yi)(yi)(yi)過(guo)濾的(de)(de)(de)(de)(de)(de)(de)(de)方(fang)法(fa)(fa)去(qu)(qu)除這些(xie)絮(xu)(xu)(xu)狀(zhuang)(zhuang)沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)，因(yin)(yin)(yin)為(wei)它可(ke)(ke)能(neng)會(hui)(hui)阻塞過(guo)濾膜(mo)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)注(zhu)(zhu)意(yi)事(shi)項：防止(zhi)發(fa)病(bing)免(mian)(mian)疫血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)多(duo)(duo)用(yong)(yong)(yong)(yong)(yong)馬(ma)、牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)制備，馬(ma)、牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)對(dui)(dui)其它動(dong)物(wu)(wu)(wu)(wu)來(lai)(lai)說，也具有(you)(you)(you)(you)抗原(yuan)(yuan)性，有(you)(you)(you)(you)引起血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病(bing)的(de)(de)(de)(de)(de)(de)(de)(de)可(ke)(ke)能(neng)，因(yin)(yin)(yin)此(ci)，使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)免(mian)(mian)疫血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)要(yao)(yao)注(zhu)(zhu)意(yi)防止(zhi)引起血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病(bing)，預防的(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)(yao)措(cuo)施是(shi)(shi)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)提純的(de)(de)(de)(de)(de)(de)(de)(de)制品(pin)，不(bu)(bu)用(yong)(yong)(yong)(yong)(yong)不(bu)(bu)合(he)格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)產(chan)(chan)(chan)(chan)品(pin)；另(ling)外，不(bu)(bu)要(yao)(yao)有(you)(you)(you)(you)急功近利的(de)(de)(de)(de)(de)(de)(de)(de)想(xiang)法(fa)(fa)，要(yao)(yao)按照(zhao)要(yao)(yao)求劑(ji)量(liang)(liang)(liang)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)，一(yi)(yi)(yi)(yi)次用(yong)(yong)(yong)(yong)(yong)量(liang)(liang)(liang)不(bu)(bu)可(ke)(ke)過(guo)大。Tips：1. 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)(bao)存(cun)：在(zai)(zai)(zai)-15℃以(yi)(yi)(yi)(yi)(yi)下(xia)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)(you)(you)效期為(wei)5年，4℃可(ke)(ke)保(bao)(bao)(bao)存(cun)數(shu)周，不(bu)(bu)宜室(shi)(shi)溫(wen)(wen)保(bao)(bao)(bao)存(cun)，不(bu)(bu)宜反復凍(dong)(dong)融(rong)(rong)。配制成(cheng)(cheng)(cheng)完(wan)(wan)(wan)全(quan)培(pei)養(yang)(yang)基后應在(zai)(zai)(zai)四周內用(yong)(yong)(yong)(yong)(yong)完(wan)(wan)(wan)，一(yi)(yi)(yi)(yi)瓶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)如(ru)無(wu)(wu)(wu)法(fa)(fa)一(yi)(yi)(yi)(yi)次用(yong)(yong)(yong)(yong)(yong)完(wan)(wan)(wan)應無(wu)(wu)(wu)菌(jun)分(fen)(fen)(fen)(fen)裝成(cheng)(cheng)(cheng)恰(qia)當數(shu)量(liang)(liang)(liang)并(bing)冷(leng)凍(dong)(dong)保(bao)(bao)(bao)存(cun)。2、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)絮(xu)(xu)(xu)狀(zhuang)(zhuang)沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)多(duo)(duo)種蛋(dan)(dan)白(bai)(bai)(bai)質的(de)(de)(de)(de)(de)(de)(de)(de)混(hun)(hun)合(he)物(wu)(wu)(wu)(wu)，在(zai)(zai)(zai)凍(dong)(dong)融(rong)(rong)過(guo)程(cheng)中(zhong)(zhong)(zhong)會(hui)(hui)出(chu)現(xian)(xian)絮(xu)(xu)(xu)狀(zhuang)(zhuang)沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)，主要(yao)(yao)原(yuan)(yuan)因(yin)(yin)(yin)是(shi)(shi)由于血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)脂蛋(dan)(dan)白(bai)(bai)(bai)和纖(xian)(xian)維(wei)蛋(dan)(dan)白(bai)(bai)(bai)變(bian)性所(suo)(suo)造成(cheng)(cheng)(cheng)。但(dan)并(bing)不(bu)(bu)影響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本身的(de)(de)(de)(de)(de)(de)(de)(de)質量(liang)(liang)(liang)和使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。如(ru)需(xu)去(qu)(qu)除可(ke)(ke)先400g離心(xin)(xin)取上清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)配制成(cheng)(cheng)(cheng)完(wan)(wan)(wan)全(quan)培(pei)養(yang)(yang)基再過(guo)濾即可(ke)(ke)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。3、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)解(jie)(jie)(jie)(jie)凍(dong)(dong)：合(he)理(li)的(de)(de)(de)(de)(de)(de)(de)(de)解(jie)(jie)(jie)(jie)凍(dong)(dong)方(fang)式可(ke)(ke)以(yi)(yi)(yi)(yi)(yi)更好的(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)(bao)護血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質量(liang)(liang)(liang)并(bing)可(ke)(ke)以(yi)(yi)(yi)(yi)(yi)在(zai)(zai)(zai)＊大程(cheng)度(du)(du)上減少絮(xu)(xu)(xu)狀(zhuang)(zhuang)沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)產(chan)(chan)(chan)(chan)生(sheng)(sheng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從低(di)溫(wen)(wen)冰箱(xiang)中(zhong)(zhong)(zhong)取出(chu)后，經4℃冰箱(xiang)、室(shi)(shi)溫(wen)(wen)、37℃水(shui)(shui)浴(yu)逐(zhu)步(bu)規則搖(yao)晃均(jun)(jun)(jun)勻(yun)(yun)(yun)(yun)(yun)融(rong)(rong)解(jie)(jie)(jie)(jie)，直至(zhi)(zhi)(zhi)完(wan)(wan)(wan)全(quan)融(rong)(rong)解(jie)(jie)(jie)(jie)后再使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。4、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)熱(re)(re)(re)(re)滅(mie)活(huo)(huo)(huo)(huo)：將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)嚴(yan)格(ge)(ge)56℃水(shui)(shui)浴(yu)均(jun)(jun)(jun)勻(yun)(yun)(yun)(yun)(yun)加溫(wen)(wen)30分(fen)(fen)(fen)(fen)鐘可(ke)(ke)以(yi)(yi)(yi)(yi)(yi)滅(mie)活(huo)(huo)(huo)(huo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)補體(ti)系統。是(shi)(shi)不(bu)(bu)是(shi)(shi)所(suo)(suo)有(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)途的(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都需(xu)要(yao)(yao)熱(re)(re)(re)(re)滅(mie)活(huo)(huo)(huo)(huo)呢？對(dui)(dui)大多(duo)(duo)數(shu)細(xi)(xi)胞(bao)(bao)(bao)來(lai)(lai)說是(shi)(shi)不(bu)(bu)需(xu)要(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)。激(ji)活(huo)(huo)(huo)(huo)的(de)(de)(de)(de)(de)(de)(de)(de)補體(ti)能(neng)參與溶(rong)解(jie)(jie)(jie)(jie)細(xi)(xi)胞(bao)(bao)(bao)，刺(ci)激(ji)光滑肌收縮，細(xi)(xi)胞(bao)(bao)(bao)和血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小板(ban)釋(shi)放組胺(an)，激(ji)活(huo)(huo)(huo)(huo)淋巴細(xi)(xi)胞(bao)(bao)(bao)和巨噬細(xi)(xi)胞(bao)(bao)(bao)。因(yin)(yin)(yin)此(ci)在(zai)(zai)(zai)免(mian)(mian)疫學(xue)研(yan)究，培(pei)養(yang)(yang)ES細(xi)(xi)胞(bao)(bao)(bao)，昆蟲細(xi)(xi)胞(bao)(bao)(bao)和平滑肌肉細(xi)(xi)胞(bao)(bao)(bao)時(shi)，建議(yi)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)熱(re)(re)(re)(re)滅(mie)活(huo)(huo)(huo)(huo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，在(zai)(zai)(zai)正(zheng)(zheng)常(chang)情況下(xia)加熱(re)(re)(re)(re)處(chu)(chu)理(li)對(dui)(dui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)促細(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)長效應無(wu)(wu)(wu)明顯促進(jin)(jin)作用(yong)(yong)(yong)(yong)(yong)，甚至(zhi)(zhi)(zhi)會(hui)(hui)因(yin)(yin)(yin)為(wei)加熱(re)(re)(re)(re)處(chu)(chu)理(li)影響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)質量(liang)(liang)(liang)。5、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)“小黑點(dian)(dian)”現(xian)(xian)象：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)經過(guo)一(yi)(yi)(yi)(yi)段時(shi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)低(di)溫(wen)(wen)凍(dong)(dong)存(cun)再融(rong)(rong)解(jie)(jie)(jie)(jie)后，在(zai)(zai)(zai)正(zheng)(zheng)常(chang)情況下(xia)會(hui)(hui)出(chu)現(xian)(xian)絮(xu)(xu)(xu)狀(zhuang)(zhuang)蛋(dan)(dan)白(bai)(bai)(bai)沉(chen)淀(dian)(dian)(dian)。但(dan)往往有(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)戶反應血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)有(you)(you)(you)(you)“小黑點(dian)(dian)”現(xian)(xian)象，認為(wei)是(shi)(shi)微(wei)生(sheng)(sheng)物(wu)(wu)(wu)(wu)污染(ran)。實驗(yan)發(fa)現(xian)(xian)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)37℃環境放置時(shi)間(jian)過(guo)長或(huo)經過(guo)加熱(re)(re)(re)(re)滅(mie)活(huo)(huo)(huo)(huo)處(chu)(chu)理(li)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)往往會(hui)(hui)呈增多(duo)(duo)趨勢(shi)，這些(xie)沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)在(zai)(zai)(zai)倒(dao)置顯微(wei)鏡下(xia)觀察，像(xiang)是(shi)(shi)“小黑點(dian)(dian)”，讓使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)者(zhe)誤以(yi)(yi)(yi)(yi)(yi)為(wei)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)遭受微(wei)生(sheng)(sheng)物(wu)(wu)(wu)(wu)污染(ran)并(bing)在(zai)(zai)(zai)分(fen)(fen)(fen)(fen)裂增殖。因(yin)(yin)(yin)此(ci)如(ru)無(wu)(wu)(wu)必要(yao)(yao)，完(wan)(wan)(wan)全(quan)可(ke)(ke)以(yi)(yi)(yi)(yi)(yi)不(bu)(bu)進(jin)(jin)行熱(re)(re)(re)(re)滅(mie)活(huo)(huo)(huo)(huo)處(chu)(chu)理(li)并(bing)保(bao)(bao)(bao)證血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)低(di)溫(wen)(wen)保(bao)(bao)(bao)存(cun)。實驗(yan)證明使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)注(zhu)(zhu)意(yi)以(yi)(yi)(yi)(yi)(yi)下(xia)幾點(dian)(dian)事(shi)項不(bu)(bu)但(dan)可(ke)(ke)以(yi)(yi)(yi)(yi)(yi)保(bao)(bao)(bao)證血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質量(liang)(liang)(liang)并(bing)能(neng)給使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)者(zhe)帶來(lai)(lai)方(fang)便(bian)：◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)在(zai)(zai)(zai)-15℃以(yi)(yi)(yi)(yi)(yi)下(xia)低(di)溫(wen)(wen)保(bao)(bao)(bao)存(cun)。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)(yi)(yi)(yi)瓶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)(yi)(yi)(yi)次無(wu)(wu)(wu)法(fa)(fa)用(yong)(yong)(yong)(yong)(yong)完(wan)(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)應分(fen)(fen)(fen)(fen)裝凍(dong)(dong)存(cun)，分(fen)(fen)(fen)(fen)次用(yong)(yong)(yong)(yong)(yong)完(wan)(wan)(wan)。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)按-15℃以(yi)(yi)(yi)(yi)(yi)下(xia)至(zhi)(zhi)(zhi)4℃至(zhi)(zhi)(zhi)室(shi)(shi)溫(wen)(wen)至(zhi)(zhi)(zhi)37℃逐(zhu)步(bu)均(jun)(jun)(jun)勻(yun)(yun)(yun)(yun)(yun)解(jie)(jie)(jie)(jie)凍(dong)(dong)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。◎若(ruo)(ruo)非必要(yao)(yao)，無(wu)(wu)(wu)須(xu)(xu)進(jin)(jin)行熱(re)(re)(re)(re)滅(mie)活(huo)(huo)(huo)(huo)。若(ruo)(ruo)必須(xu)(xu)做(zuo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)熱(re)(re)(re)(re)滅(mie)活(huo)(huo)(huo)(huo)的(de)(de)(de)(de)(de)(de)(de)(de)，須(xu)(xu)嚴(yan)格(ge)(ge)控(kong)制在(zai)(zai)(zai)56℃水(shui)(shui)浴(yu)30分(fen)(fen)(fen)(fen)鐘均(jun)(jun)(jun)勻(yun)(yun)(yun)(yun)(yun)加熱(re)(re)(re)(re)滅(mie)活(huo)(huo)(huo)(huo)。胎(tai)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿（去(qu)(qu)紅細(xi)(xi)胞(bao)(bao)(bao)、無(wu)(wu)(wu)菌(jun)過(guo)濾） 主要(yao)(yao)成(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)： 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)由血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿去(qu)(qu)除纖(xian)(xian)維(wei)蛋(dan)(dan)白(bai)(bai)(bai)原(yuan)(yuan)而(er)(er)形成(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)種很復雜(za)的(de)(de)(de)(de)(de)(de)(de)(de)混(hun)(hun)合(he)物(wu)(wu)(wu)(wu)，其組成(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)份(fen)雖大部(bu)分(fen)(fen)(fen)(fen)已知，但(dan)還有(you)(you)(you)(you)一(yi)(yi)(yi)(yi)部(bu)分(fen)(fen)(fen)(fen)尚不(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組成(cheng)(cheng)(cheng)及(ji)含量(liang)(liang)(liang)常(chang)隨供(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)性別、年齡、生(sheng)(sheng)li條(tiao)件(jian)和營(ying)養(yang)(yang)條(tiao)件(jian)不(bu)(bu)同而(er)(er)異。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿中(zhong)(zhong)(zhong)不(bu)(bu)含纖(xian)(xian)維(wei)蛋(dan)(dan)白(bai)(bai)(bai)原(yuan)(yuan)的(de)(de)(de)(de)(de)(de)(de)(de)膠狀(zhuang)(zhuang)液(ye)(ye)體(ti)，具有(you)(you)(you)(you)維(wei)持血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)正(zheng)(zheng)常(chang)粘度(du)(du)、酸堿度(du)(du)、滲透(tou)壓等(deng)(deng)作用(yong)(yong)(yong)(yong)(yong)。它主要(yao)(yao)由水(shui)(shui)和各(ge)種化學(xue)成(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)組成(cheng)(cheng)(cheng)，這些(xie)化學(xue)成(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)包(bao)括白(bai)(bai)(bai)蛋(dan)(dan)白(bai)(bai)(bai)、α1、α2、β、γ-球蛋(dan)(dan)白(bai)(bai)(bai)，甘油三(san)酯，總(zong)膽固醇(chun)，谷丙轉氨酶等(deng)(deng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)含有(you)(you)(you)(you)各(ge)種血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿蛋(dan)(dan)白(bai)(bai)(bai)、多(duo)(duo)肽、脂肪、碳水(shui)(shui)化合(he)物(wu)(wu)(wu)(wu)、生(sheng)(sheng)長因(yin)(yin)(yin)子(zi)、激(ji)素、無(wu)(wu)(wu)機物(wu)(wu)(wu)(wu)等(deng)(deng)，這些(xie)物(wu)(wu)(wu)(wu)質對(dui)(dui)促進(jin)(jin)細(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)長或(huo)抑制生(sheng)(sheng)長活(huo)(huo)(huo)(huo)性是(shi)(shi)達到生(sheng)(sheng)li平衡的(de)(de)(de)(de)(de)(de)(de)(de)。
大(da)牛(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)（去(qu)紅細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)、無(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)過(guo)濾(lv)）產(chan)(chan)(chan)(chan)(chan)(chan)品(pin)規格：100ml/200ml/500ml保(bao) 存(cun)(cun)：-20℃，三年。 使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)時(shi)(shi)(shi)(shi)(shi)常(chang)(chang)(chang)見問題及(ji)(ji)解(jie)(jie)決方(fang)(fang)法(fa)：一(yi)(yi)(yi)、保(bao)存(cun)(cun)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)法(fa)?血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)應(ying)(ying)(ying)保(bao)存(cun)(cun)在(zai)(zai)(zai)(zai)(zai)(zai)-5℃至-2O℃若(ruo)(ruo)存(cun)(cun)放(fang)(fang)于(yu)(yu)4℃時(shi)(shi)(shi)(shi)(shi)，請(qing)勿超過(guo)一(yi)(yi)(yi)個(ge)月。若(ruo)(ruo)您一(yi)(yi)(yi)次(ci)(ci)無(wu)(wu)(wu)法(fa)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)(wan)一(yi)(yi)(yi)瓶(ping)，建議將(jiang)(jiang)無(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)分(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)至恰當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)滅(mie)(mie)(mie)(mie)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)容(rong)器(qi)(qi)內(nei)(nei)(nei)，再(zai)(zai)放(fang)(fang)回冷(leng)凍(dong)(dong)(dong)。二、如(ru)(ru)何解(jie)(jie)凍(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)才不(bu)(bu)(bu)(bu)(bu)會(hui)(hui)(hui)(hui)(hui)(hui)(hui)使(shi)(shi)(shi)(shi)產(chan)(chan)(chan)(chan)(chan)(chan)品(pin)質(zhi)(zhi)量(liang)(liang)受(shou)損?將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)(cong)冷(leng)凍(dong)(dong)(dong)箱取(qu)出后(hou)(hou)(hou)(hou)，先置于(yu)(yu)2-8℃冰箱使(shi)(shi)(shi)(shi)之溶解(jie)(jie)，然(ran)后(hou)(hou)(hou)(hou)在(zai)(zai)(zai)(zai)(zai)(zai)室(shi)溫(wen)(wen)下(xia)(xia)使(shi)(shi)(shi)(shi)之全(quan)溶。但(dan)必(bi)(bi)(bi)須(xu)(xu)注(zhu)(zhu)(zhu)意(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)，溶解(jie)(jie)過(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)必(bi)(bi)(bi)須(xu)(xu)規則(ze)(ze)地搖(yao)(yao)晃均(jun)(jun)勻(yun)。三、如(ru)(ru)何避免(mian)沉(chen)(chen)(chen)(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)(chan)(chan)(chan)(chan)(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)?1.解(jie)(jie)凍(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)(shi)(shi)(shi)，請(qing)按(an)照所建議的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)逐(zhu)步(bu)(bu)解(jie)(jie)凍(dong)(dong)(dong)法(fa)(-2O℃至4℃至室(shi)溫(wen)(wen))，若(ruo)(ruo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)凍(dong)(dong)(dong)時(shi)(shi)(shi)(shi)(shi)改(gai)變(bian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)溫(wen)(wen)度(du)太(tai)(tai)大(da)(如(ru)(ru)-20℃至37℃)，實驗顯示(shi)非(fei)常(chang)(chang)(chang)容(rong)易(yi)產(chan)(chan)(chan)(chan)(chan)(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)沉(chen)(chen)(chen)(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)。2.解(jie)(jie)凍(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)(shi)(shi)(shi)，請(qing)隨時(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)之搖(yao)(yao)晃均(jun)(jun)勻(yun)，使(shi)(shi)(shi)(shi)溫(wen)(wen)度(du)及(ji)(ji)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)均(jun)(jun)一(yi)(yi)(yi)，減少沉(chen)(chen)(chen)(chen)(chen)淀(dian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)3.請(qing)勿將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)置于(yu)(yu)37℃太(tai)(tai)久。若(ruo)(ruo)在(zai)(zai)(zai)(zai)(zai)(zai)37℃放(fang)(fang)置太(tai)(tai)久，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)會(hui)(hui)(hui)(hui)(hui)(hui)(hui)變(bian)得混(hun)(hun)濁，同時(shi)(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)許多較不(bu)(bu)(bu)(bu)(bu)穩定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)也(ye)會(hui)(hui)(hui)(hui)(hui)(hui)(hui)因(yin)(yin)(yin)(yin)(yin)此(ci)(ci)(ci)受(shou)到(dao)(dao)損害(hai)，而影響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)量(liang)(liang)。4.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱(re)(re)(re)(re)滅(mie)(mie)(mie)(mie)活(huo)(huo)非(fei)常(chang)(chang)(chang)容(rong)易(yi)造(zao)(zao)(zao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)沉(chen)(chen)(chen)(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)增多，若(ruo)(ruo)非(fei)必(bi)(bi)(bi)要(yao)(yao)(yao)，可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)無(wu)(wu)(wu)須(xu)(xu)做(zuo)(zuo)(zuo)此(ci)(ci)(ci)步(bu)(bu)驟(zou)(zou)。5.若(ruo)(ruo)必(bi)(bi)(bi)須(xu)(xu)做(zuo)(zuo)(zuo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱(re)(re)(re)(re)滅(mie)(mie)(mie)(mie)活(huo)(huo)，請(qing)遵守56℃，30分(fen)(fen)(fen)(fen)(fen)(fen)鐘的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原則(ze)(ze)，并(bing)(bing)(bing)(bing)且隨時(shi)(shi)(shi)(shi)(shi)搖(yao)(yao)晃均(jun)(jun)勻(yun)。溫(wen)(wen)度(du)過(guo)高(gao)(gao)，時(shi)(shi)(shi)(shi)(shi)間(jian)過(guo)久或(huo)(huo)(huo)搖(yao)(yao)晃不(bu)(bu)(bu)(bu)(bu)均(jun)(jun)勻(yun)，都(dou)會(hui)(hui)(hui)(hui)(hui)(hui)(hui)造(zao)(zao)(zao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)沉(chen)(chen)(chen)(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)增多。四、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)凍(dong)(dong)(dong)后(hou)(hou)(hou)(hou)發現(xian)(xian)有(you)(you)(you)(you)(you)絮狀沉(chen)(chen)(chen)(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)出現(xian)(xian)，該如(ru)(ru)何處(chu)(chu)理(li)(li)? 欲去(qu)除(chu)(chu)這些(xie)(xie)(xie)絮狀沉(chen)(chen)(chen)(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)，可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)分(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)至無(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)離心管(guan)(guan)(guan)內(nei)(nei)(nei)，以(yi)(yi)(yi)(yi)400g稍微離心，上(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)即可(ke)(ke)(ke)(ke)(ke)(ke)接著加(jia)(jia)入(ru)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)內(nei)(nei)(nei)一(yi)(yi)(yi)起(qi)過(guo)濾(lv)。但(dan)不(bu)(bu)(bu)(bu)(bu)建議以(yi)(yi)(yi)(yi)過(guo)濾(lv)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)法(fa)去(qu)除(chu)(chu)這些(xie)(xie)(xie)絮狀沉(chen)(chen)(chen)(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)，因(yin)(yin)(yin)(yin)(yin)為(wei)(wei)它(ta)(ta)可(ke)(ke)(ke)(ke)(ke)(ke)能會(hui)(hui)(hui)(hui)(hui)(hui)(hui)阻塞(sai)過(guo)濾(lv)膜。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)注(zhu)(zhu)(zhu)意(yi)(yi)事(shi)項：防(fang)止(zhi)發病(bing)(bing)免(mian)疫血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)多用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)馬、牛(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)制(zhi)備，馬、牛(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)對其它(ta)(ta)動(dong)物(wu)(wu)(wu)(wu)來說，也(ye)具有(you)(you)(you)(you)(you)抗(kang)原性，有(you)(you)(you)(you)(you)引起(qi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病(bing)(bing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)可(ke)(ke)(ke)(ke)(ke)(ke)能，因(yin)(yin)(yin)(yin)(yin)此(ci)(ci)(ci)，使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)免(mian)疫血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)要(yao)(yao)(yao)注(zhu)(zhu)(zhu)意(yi)(yi)防(fang)止(zhi)引起(qi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病(bing)(bing)，預防(fang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)(yao)(yao)措施是(shi)(shi)(shi)(shi)(shi)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)提純的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)品(pin)，不(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)合(he)格的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)(chan)(chan)(chan)(chan)(chan)品(pin)；另外，不(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)有(you)(you)(you)(you)(you)急(ji)功近利的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)想(xiang)法(fa)，要(yao)(yao)(yao)按(an)照要(yao)(yao)(yao)求劑量(liang)(liang)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，一(yi)(yi)(yi)次(ci)(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)量(liang)(liang)不(bu)(bu)(bu)(bu)(bu)可(ke)(ke)(ke)(ke)(ke)(ke)過(guo)大(da)。Tips：1. 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)存(cun)(cun)：在(zai)(zai)(zai)(zai)(zai)(zai)-15℃以(yi)(yi)(yi)(yi)下(xia)(xia)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)(you)(you)(you)效(xiao)期為(wei)(wei)5年，4℃可(ke)(ke)(ke)(ke)(ke)(ke)保(bao)存(cun)(cun)數周(zhou)，不(bu)(bu)(bu)(bu)(bu)宜室(shi)溫(wen)(wen)保(bao)存(cun)(cun)，不(bu)(bu)(bu)(bu)(bu)宜反(fan)復凍(dong)(dong)(dong)融(rong)(rong)。配(pei)(pei)制(zhi)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)完(wan)(wan)(wan)全(quan)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)后(hou)(hou)(hou)(hou)應(ying)(ying)(ying)在(zai)(zai)(zai)(zai)(zai)(zai)四周(zhou)內(nei)(nei)(nei)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)(wan)，一(yi)(yi)(yi)瓶(ping)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)如(ru)(ru)無(wu)(wu)(wu)法(fa)一(yi)(yi)(yi)次(ci)(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)(wan)應(ying)(ying)(ying)無(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)分(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)恰當數量(liang)(liang)并(bing)(bing)(bing)(bing)冷(leng)凍(dong)(dong)(dong)保(bao)存(cun)(cun)。2、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)絮狀沉(chen)(chen)(chen)(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)多種(zhong)(zhong)(zhong)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混(hun)(hun)合(he)物(wu)(wu)(wu)(wu)，在(zai)(zai)(zai)(zai)(zai)(zai)凍(dong)(dong)(dong)融(rong)(rong)過(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)會(hui)(hui)(hui)(hui)(hui)(hui)(hui)出現(xian)(xian)絮狀沉(chen)(chen)(chen)(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)，主要(yao)(yao)(yao)原因(yin)(yin)(yin)(yin)(yin)是(shi)(shi)(shi)(shi)(shi)由(you)于(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)和(he)(he)(he)(he)纖(xian)(xian)維(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)變(bian)性所造(zao)(zao)(zao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)。但(dan)并(bing)(bing)(bing)(bing)不(bu)(bu)(bu)(bu)(bu)影響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本身的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)量(liang)(liang)和(he)(he)(he)(he)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。如(ru)(ru)需(xu)(xu)去(qu)除(chu)(chu)可(ke)(ke)(ke)(ke)(ke)(ke)先400g離心取(qu)上(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)配(pei)(pei)制(zhi)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)完(wan)(wan)(wan)全(quan)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)再(zai)(zai)過(guo)濾(lv)即可(ke)(ke)(ke)(ke)(ke)(ke)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。3、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)(jie)凍(dong)(dong)(dong)：合(he)理(li)(li)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)(jie)凍(dong)(dong)(dong)方(fang)(fang)式可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)更(geng)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)護(hu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)量(liang)(liang)并(bing)(bing)(bing)(bing)可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)在(zai)(zai)(zai)(zai)(zai)(zai)＊大(da)程(cheng)度(du)上(shang)減少絮狀沉(chen)(chen)(chen)(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)(chan)(chan)(chan)(chan)(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)(cong)低(di)溫(wen)(wen)冰箱中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)取(qu)出后(hou)(hou)(hou)(hou)，經(jing)4℃冰箱、室(shi)溫(wen)(wen)、37℃水(shui)(shui)(shui)浴逐(zhu)步(bu)(bu)規則(ze)(ze)搖(yao)(yao)晃均(jun)(jun)勻(yun)融(rong)(rong)解(jie)(jie)，直至完(wan)(wan)(wan)全(quan)融(rong)(rong)解(jie)(jie)后(hou)(hou)(hou)(hou)再(zai)(zai)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。4、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱(re)(re)(re)(re)滅(mie)(mie)(mie)(mie)活(huo)(huo)：將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)嚴格56℃水(shui)(shui)(shui)浴均(jun)(jun)勻(yun)加(jia)(jia)溫(wen)(wen)30分(fen)(fen)(fen)(fen)(fen)(fen)鐘可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)滅(mie)(mie)(mie)(mie)活(huo)(huo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補(bu)體(ti)系(xi)統(tong)。是(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)是(shi)(shi)(shi)(shi)(shi)所有(you)(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)途的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都(dou)需(xu)(xu)要(yao)(yao)(yao)熱(re)(re)(re)(re)滅(mie)(mie)(mie)(mie)活(huo)(huo)呢？對大(da)多數細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)來說是(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)需(xu)(xu)要(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。激活(huo)(huo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補(bu)體(ti)能參與溶解(jie)(jie)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，刺(ci)激光(guang)滑肌(ji)收(shou)縮，細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)和(he)(he)(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小板釋放(fang)(fang)組胺，激活(huo)(huo)淋(lin)巴細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)和(he)(he)(he)(he)巨噬細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)。因(yin)(yin)(yin)(yin)(yin)此(ci)(ci)(ci)在(zai)(zai)(zai)(zai)(zai)(zai)免(mian)疫學(xue)研究，培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)ES細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，昆蟲(chong)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)和(he)(he)(he)(he)平滑肌(ji)肉細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)時(shi)(shi)(shi)(shi)(shi)，建議使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)熱(re)(re)(re)(re)滅(mie)(mie)(mie)(mie)活(huo)(huo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，在(zai)(zai)(zai)(zai)(zai)(zai)正常(chang)(chang)(chang)情(qing)況(kuang)下(xia)(xia)加(jia)(jia)熱(re)(re)(re)(re)處(chu)(chu)理(li)(li)對血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)促細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)效(xiao)應(ying)(ying)(ying)無(wu)(wu)(wu)明(ming)顯促進(jin)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，甚至會(hui)(hui)(hui)(hui)(hui)(hui)(hui)因(yin)(yin)(yin)(yin)(yin)為(wei)(wei)加(jia)(jia)熱(re)(re)(re)(re)處(chu)(chu)理(li)(li)影響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)量(liang)(liang)。5、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)“小黑點”現(xian)(xian)象：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)(zai)(zai)經(jing)過(guo)一(yi)(yi)(yi)段時(shi)(shi)(shi)(shi)(shi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低(di)溫(wen)(wen)凍(dong)(dong)(dong)存(cun)(cun)再(zai)(zai)融(rong)(rong)解(jie)(jie)后(hou)(hou)(hou)(hou)，在(zai)(zai)(zai)(zai)(zai)(zai)正常(chang)(chang)(chang)情(qing)況(kuang)下(xia)(xia)會(hui)(hui)(hui)(hui)(hui)(hui)(hui)出現(xian)(xian)絮狀蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)沉(chen)(chen)(chen)(chen)(chen)淀(dian)。但(dan)往(wang)往(wang)有(you)(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶反(fan)應(ying)(ying)(ying)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)(you)(you)“小黑點”現(xian)(xian)象，認為(wei)(wei)是(shi)(shi)(shi)(shi)(shi)微生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)污染(ran)。實驗發現(xian)(xian)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)(zai)(zai)37℃環(huan)境放(fang)(fang)置時(shi)(shi)(shi)(shi)(shi)間(jian)過(guo)長(chang)或(huo)(huo)(huo)經(jing)過(guo)加(jia)(jia)熱(re)(re)(re)(re)滅(mie)(mie)(mie)(mie)活(huo)(huo)處(chu)(chu)理(li)(li)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)沉(chen)(chen)(chen)(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)往(wang)往(wang)會(hui)(hui)(hui)(hui)(hui)(hui)(hui)呈增多趨勢，這些(xie)(xie)(xie)沉(chen)(chen)(chen)(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)在(zai)(zai)(zai)(zai)(zai)(zai)倒置顯微鏡下(xia)(xia)觀(guan)察(cha)，像是(shi)(shi)(shi)(shi)(shi)“小黑點”，讓使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)者誤(wu)以(yi)(yi)(yi)(yi)為(wei)(wei)是(shi)(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)遭受(shou)微生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)污染(ran)并(bing)(bing)(bing)(bing)在(zai)(zai)(zai)(zai)(zai)(zai)分(fen)(fen)(fen)(fen)(fen)(fen)裂增殖。因(yin)(yin)(yin)(yin)(yin)此(ci)(ci)(ci)如(ru)(ru)無(wu)(wu)(wu)必(bi)(bi)(bi)要(yao)(yao)(yao)，完(wan)(wan)(wan)全(quan)可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)不(bu)(bu)(bu)(bu)(bu)進(jin)行(xing)熱(re)(re)(re)(re)滅(mie)(mie)(mie)(mie)活(huo)(huo)處(chu)(chu)理(li)(li)并(bing)(bing)(bing)(bing)保(bao)證(zheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低(di)溫(wen)(wen)保(bao)存(cun)(cun)。實驗證(zheng)明(ming)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)(shi)(shi)(shi)注(zhu)(zhu)(zhu)意(yi)(yi)以(yi)(yi)(yi)(yi)下(xia)(xia)幾(ji)點事(shi)項不(bu)(bu)(bu)(bu)(bu)但(dan)可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)保(bao)證(zheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)量(liang)(liang)并(bing)(bing)(bing)(bing)能給使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)者帶(dai)來方(fang)(fang)便：◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)(ke)(ke)(ke)(ke)在(zai)(zai)(zai)(zai)(zai)(zai)-15℃以(yi)(yi)(yi)(yi)下(xia)(xia)低(di)溫(wen)(wen)保(bao)存(cun)(cun)。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)(yi)(yi)瓶(ping)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)(yi)(yi)次(ci)(ci)無(wu)(wu)(wu)法(fa)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)(ying)(ying)分(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)凍(dong)(dong)(dong)存(cun)(cun)，分(fen)(fen)(fen)(fen)(fen)(fen)次(ci)(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)(wan)。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)按(an)-15℃以(yi)(yi)(yi)(yi)下(xia)(xia)至4℃至室(shi)溫(wen)(wen)至37℃逐(zhu)步(bu)(bu)均(jun)(jun)勻(yun)解(jie)(jie)凍(dong)(dong)(dong)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。◎若(ruo)(ruo)非(fei)必(bi)(bi)(bi)要(yao)(yao)(yao)，無(wu)(wu)(wu)須(xu)(xu)進(jin)行(xing)熱(re)(re)(re)(re)滅(mie)(mie)(mie)(mie)活(huo)(huo)。若(ruo)(ruo)必(bi)(bi)(bi)須(xu)(xu)做(zuo)(zuo)(zuo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)熱(re)(re)(re)(re)滅(mie)(mie)(mie)(mie)活(huo)(huo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，須(xu)(xu)嚴格控制(zhi)在(zai)(zai)(zai)(zai)(zai)(zai)56℃水(shui)(shui)(shui)浴30分(fen)(fen)(fen)(fen)(fen)(fen)鐘均(jun)(jun)勻(yun)加(jia)(jia)熱(re)(re)(re)(re)滅(mie)(mie)(mie)(mie)活(huo)(huo)。大(da)牛(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)（去(qu)紅細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)、無(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)過(guo)濾(lv)）主要(yao)(yao)(yao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)： 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)由(you)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)去(qu)除(chu)(chu)纖(xian)(xian)維(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)原而形(xing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)種(zhong)(zhong)(zhong)很復雜的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混(hun)(hun)合(he)物(wu)(wu)(wu)(wu)，其組成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)份雖大(da)部分(fen)(fen)(fen)(fen)(fen)(fen)已(yi)知，但(dan)還(huan)有(you)(you)(you)(you)(you)一(yi)(yi)(yi)部分(fen)(fen)(fen)(fen)(fen)(fen)尚不(bu)(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)及(ji)(ji)含量(liang)(liang)常(chang)(chang)(chang)隨供(gong)(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性別、年齡、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)li條件(jian)(jian)(jian)和(he)(he)(he)(he)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)條件(jian)(jian)(jian)不(bu)(bu)(bu)(bu)(bu)同而異。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)不(bu)(bu)(bu)(bu)(bu)含纖(xian)(xian)維(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)原的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠(jiao)狀液(ye)(ye)(ye)(ye)體(ti)，具有(you)(you)(you)(you)(you)維(wei)(wei)(wei)(wei)持血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正常(chang)(chang)(chang)粘(zhan)度(du)、酸(suan)堿(jian)度(du)、滲透壓等(deng)(deng)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。它(ta)(ta)主要(yao)(yao)(yao)由(you)水(shui)(shui)(shui)和(he)(he)(he)(he)各(ge)種(zhong)(zhong)(zhong)化(hua)(hua)學(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)組成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)，這些(xie)(xie)(xie)化(hua)(hua)學(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)包括(kuo)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)、α1、α2、β、γ-球蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)，甘油(you)三酯，總膽固(gu)醇(chun)，谷丙轉氨酶(mei)(mei)(mei)等(deng)(deng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)含有(you)(you)(you)(you)(you)各(ge)種(zhong)(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)、多肽(tai)、脂(zhi)(zhi)肪、碳水(shui)(shui)(shui)化(hua)(hua)合(he)物(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)(yin)(yin)子、激素(su)、無(wu)(wu)(wu)機(ji)物(wu)(wu)(wu)(wu)等(deng)(deng)，這些(xie)(xie)(xie)物(wu)(wu)(wu)(wu)質(zhi)(zhi)對促進(jin)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)或(huo)(huo)(huo)抑制(zhi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)活(huo)(huo)性是(shi)(shi)(shi)(shi)(shi)達到(dao)(dao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)li平衡(heng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)(yao)(yao)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)●提供(gong)(gong)基(ji)(ji)(ji)(ji)本營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)質(zhi)(zhi)：氨基(ji)(ji)(ji)(ji)酸(suan)、維(wei)(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素(su)、無(wu)(wu)(wu)機(ji)物(wu)(wu)(wu)(wu)、脂(zhi)(zhi)類物(wu)(wu)(wu)(wu)質(zhi)(zhi)、核酸(suan)衍生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)等(deng)(deng)，是(shi)(shi)(shi)(shi)(shi)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)必(bi)(bi)(bi)須(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)質(zhi)(zhi)。●提供(gong)(gong)激素(su)和(he)(he)(he)(he)各(ge)種(zhong)(zhong)(zhong)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)(yin)(yin)子：胰島素(su)、腎上(shang)腺皮質(zhi)(zhi)激素(su)（氫化(hua)(hua)可(ke)(ke)(ke)(ke)(ke)(ke)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)松、地塞(sai)米松）、類固(gu)醇(chun)激素(su)（雌(ci)二醇(chun)、睪酮、孕酮）等(deng)(deng)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)(yin)(yin)子如(ru)(ru)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)纖(xian)(xian)維(wei)(wei)(wei)(wei)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)(yin)(yin)子、表(biao)皮生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)(yin)(yin)子、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小板生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)(yin)(yin)子等(deng)(deng)。●提供(gong)(gong)結(jie)(jie)合(he)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)：結(jie)(jie)合(he)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)(shi)(shi)攜帶(dai)重(zhong)(zhong)要(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低(di)分(fen)(fen)(fen)(fen)(fen)(fen)子量(liang)(liang)物(wu)(wu)(wu)(wu)質(zhi)(zhi)，如(ru)(ru)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜帶(dai)維(wei)(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素(su)、脂(zhi)(zhi)肪、以(yi)(yi)(yi)(yi)及(ji)(ji)激素(su)等(deng)(deng)，轉鐵(tie)(tie)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜帶(dai)鐵(tie)(tie)。結(jie)(jie)合(he)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)在(zai)(zai)(zai)(zai)(zai)(zai)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)代(dai)謝過(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)起(qi)重(zhong)(zhong)要(yao)(yao)(yao)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。●提供(gong)(gong)促接觸和(he)(he)(he)(he)伸展因(yin)(yin)(yin)(yin)(yin)子使(shi)(shi)(shi)(shi)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)貼(tie)壁(bi)免(mian)受(shou)機(ji)械(xie)損傷。●對培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)起(qi)到(dao)(dao)某(mou)些(xie)(xie)(xie)保(bao)護(hu)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)：有(you)(you)(you)(you)(you)一(yi)(yi)(yi)些(xie)(xie)(xie)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，如(ru)(ru)內(nei)(nei)(nei)皮細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)、骨髓(sui)樣(yang)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)釋放(fang)(fang)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)(mei)(mei)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)含有(you)(you)(you)(you)(you)抗(kang)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)(mei)(mei)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)，起(qi)到(dao)(dao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。這種(zhong)(zhong)(zhong)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)(shi)(shi)偶然(ran)發現(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，則(ze)(ze)有(you)(you)(you)(you)(you)目(mu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)來終(zhong)止(zhi)胰蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消(xiao)化(hua)(hua)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。因(yin)(yin)(yin)(yin)(yin)為(wei)(wei)胰蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)(mei)(mei)已(yi)經(jing)被廣泛用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)貼(tie)壁(bi)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消(xiao)化(hua)(hua)傳代(dai)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)形(xing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)了血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)粘(zhan)度(du)，可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)保(bao)護(hu)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)免(mian)受(shou)機(ji)械(xie)損傷，特別是(shi)(shi)(shi)(shi)(shi)在(zai)(zai)(zai)(zai)(zai)(zai)懸浮培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)攪拌時(shi)(shi)(shi)(shi)(shi)，粘(zhan)度(du)起(qi)到(dao)(dao)重(zhong)(zhong)要(yao)(yao)(yao)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)還(huan)含有(you)(you)(you)(you)(you)一(yi)(yi)(yi)些(xie)(xie)(xie)微量(liang)(liang)元(yuan)(yuan)素(su)和(he)(he)(he)(he)離子，他們在(zai)(zai)(zai)(zai)(zai)(zai)代(dai)謝解(jie)(jie)毒中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)起(qi)重(zhong)(zhong)要(yao)(yao)(yao)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)SeO3，硒等(deng)(deng)。細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)條件(jian)(jian)(jian)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)根據(ju)細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)類和(he)(he)(he)(he)目(mu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)等(deng)(deng)選(xuan)擇培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)方(fang)(fang)法(fa)、培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)，制(zhi)定(ding)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)條件(jian)(jian)(jian)(溫(wen)(wen)度(du)、pH值、時(shi)(shi)(shi)(shi)(shi)間(jian)，對氧(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)需(xu)(xu)求與否(fou)等(deng)(deng))。一(yi)(yi)(yi)般操(cao)作(zuo)步(bu)(bu)驟(zou)(zou)為(wei)(wei)先將(jiang)(jiang)標本接種(zhong)(zhong)(zhong)于(yu)(yu)固(gu)體(ti)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)，做(zuo)(zuo)(zuo)分(fen)(fen)(fen)(fen)(fen)(fen)離培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)。再(zai)(zai)進(jin)一(yi)(yi)(yi)步(bu)(bu)對所得單(dan)個(ge)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)落進(jin)行(xing)形(xing)態(tai)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)及(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)學(xue)反(fan)應(ying)(ying)(ying)鑒(jian)定(ding)。培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)牛(niu)(niu)肉湯、蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)、氯化(hua)(hua)鈉、葡(pu)萄糖、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)等(deng)(deng)和(he)(he)(he)(he)某(mou)些(xie)(xie)(xie)細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)所需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)特殊物(wu)(wu)(wu)(wu)質(zhi)(zhi)配(pei)(pei)制(zhi)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)液(ye)(ye)(ye)(ye)體(ti)、半(ban)固(gu)體(ti)、固(gu)體(ti)等(deng)(deng)。一(yi)(yi)(yi)般細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)可(ke)(ke)(ke)(ke)(ke)(ke)在(zai)(zai)(zai)(zai)(zai)(zai)有(you)(you)(you)(you)(you)氧(yang)條件(jian)(jian)(jian)下(xia)(xia)，37℃中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)放(fang)(fang)18~24小時(shi)(shi)(shi)(shi)(shi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)。厭氧(yang)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)則(ze)(ze)需(xu)(xu)在(zai)(zai)(zai)(zai)(zai)(zai)無(wu)(wu)(wu)氧(yang)環(huan)境中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)放(fang)(fang)2~3天后(hou)(hou)(hou)(hou)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)。個(ge)別細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)如(ru)(ru)結(jie)(jie)核菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)要(yao)(yao)(yao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)1個(ge)月之久。細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)條件(jian)(jian)(jian)由(you)于(yu)(yu)細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)無(wu)(wu)(wu)處(chu)(chu)不(bu)(bu)(bu)(bu)(bu)在(zai)(zai)(zai)(zai)(zai)(zai)，因(yin)(yin)(yin)(yin)(yin)此(ci)(ci)(ci)從(cong)(cong)制(zhi)備培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)時(shi)(shi)(shi)(shi)(shi)開始(shi)，整(zheng)個(ge)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)過(guo)程(cheng)必(bi)(bi)(bi)須(xu)(xu)按(an)無(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)操(cao)作(zuo)要(yao)(yao)(yao)求進(jin)行(xing)，否(fou)則(ze)(ze)外界細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)污染(ran)標本，會(hui)(hui)(hui)(hui)(hui)(hui)(hui)導致錯誤(wu)結(jie)(jie)果;而培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)致病(bing)(bing)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)一(yi)(yi)(yi)旦污染(ran)環(huan)境，就會(hui)(hui)(hui)(hui)(hui)(hui)(hui)引起(qi)交叉(cha)感染(ran)。以(yi)(yi)(yi)(yi)疾病(bing)(bing)診斷(duan)為(wei)(wei)目(mu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)進(jin)行(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)，要(yao)(yao)(yao)選(xuan)擇合(he)適的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標本(血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)、尿、便、膿液(ye)(ye)(ye)(ye)、分(fen)(fen)(fen)(fen)(fen)(fen)泌物(wu)(wu)(wu)(wu)等(deng)(deng))，并(bing)(bing)(bing)(bing)應(ying)(ying)(ying)結(jie)(jie)合(he)臨床情(qing)況(kuang)解(jie)(jie)釋所得結(jie)(jie)果。培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)步(bu)(bu)驟(zou)(zou)以(yi)(yi)(yi)(yi)光(guang)合(he)細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)方(fang)(fang)法(fa)為(wei)(wei)例(li)。光(guang)合(he)細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)法(fa)，按(an)次(ci)(ci)序分(fen)(fen)(fen)(fen)(fen)(fen)為(wei)(wei)容(rong)器(qi)(qi)、工具的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消(xiao)毒，培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)備，接種(zhong)(zhong)(zhong)和(he)(he)(he)(he)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)管(guan)(guan)(guan)理(li)(li)四個(ge)步(bu)(bu)驟(zou)(zou)。(一(yi)(yi)(yi))容(rong)器(qi)(qi)、工具的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消(xiao)毒參考(kao)、此(ci)(ci)(ci)處(chu)(chu)從(cong)(cong)略。(二)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)備1.培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)水(shui)(shui)(shui)如(ru)(ru)果培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)合(he)細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)是(shi)(shi)(shi)(shi)(shi)淡水(shui)(shui)(shui)種(zhong)(zhong)(zhong)，菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)可(ke)(ke)(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)蒸餾(liu)水(shui)(shui)(shui)，生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)(chan)(chan)(chan)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)可(ke)(ke)(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)消(xiao)毒的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)自來水(shui)(shui)(shui)(或(huo)(huo)(huo)井水(shui)(shui)(shui))配(pei)(pei)制(zhi)。如(ru)(ru)果培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)合(he)細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)是(shi)(shi)(shi)(shi)(shi)海(hai)水(shui)(shui)(shui)種(zhong)(zhong)(zhong)，則(ze)(ze)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)天然(ran)海(hai)水(shui)(shui)(shui)配(pei)(pei)制(zhi)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)，注(zhu)(zhu)(zhu)意(yi)(yi)在(zai)(zai)(zai)(zai)(zai)(zai)海(hai)水(shui)(shui)(shui)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)入(ru)磷(lin)元(yuan)(yuan)素(su)時(shi)(shi)(shi)(shi)(shi)，不(bu)(bu)(bu)(bu)(bu)能用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)磷(lin)酸(suan)氫二鉀(jia)，應(ying)(ying)(ying)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)磷(lin)酸(suan)二氫鉀(jia)，不(bu)(bu)(bu)(bu)(bu)然(ran)會(hui)(hui)(hui)(hui)(hui)(hui)(hui)產(chan)(chan)(chan)(chan)(chan)(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)大(da)量(liang)(liang)沉(chen)(chen)(chen)(chen)(chen)淀(dian)。2.滅(mie)(mie)(mie)(mie)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)和(he)(he)(he)(he)消(xiao)毒菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)應(ying)(ying)(ying)連同培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)容(rong)器(qi)(qi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)高(gao)(gao)壓蒸氣(qi)滅(mie)(mie)(mie)(mie)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)鍋滅(mie)(mie)(mie)(mie)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)。小型生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)(chan)(chan)(chan)性培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)可(ke)(ke)(ke)(ke)(ke)(ke)把(ba)(ba)配(pei)(pei)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)液(ye)(ye)(ye)(ye)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)普(pu)通(tong)鋁(lv)鍋或(huo)(huo)(huo)大(da)型三角燒瓶(ping)煮沸消(xiao)毒。大(da)型生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)(chan)(chan)(chan)性培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)則(ze)(ze)把(ba)(ba)經(jing)沉(chen)(chen)(chen)(chen)(chen)淀(dian)砂濾(lv)后(hou)(hou)(hou)(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)水(shui)(shui)(shui)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)漂白(bai)(bai)(bai)(bai)(bai)(bai)(bai)粉(或(huo)(huo)(huo)漂白(bai)(bai)(bai)(bai)(bai)(bai)(bai)液(ye)(ye)(ye)(ye))消(xiao)毒后(hou)(hou)(hou)(hou)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。3.培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)配(pei)(pei)制(zhi)根據(ju)所培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)種(zhong)(zhong)(zhong)類的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)需(xu)(xu)要(yao)(yao)(yao)選(xuan)擇合(he)適的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)配(pei)(pei)方(fang)(fang)。按(an)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)配(pei)(pei)方(fang)(fang)把(ba)(ba)所需(xu)(xu)物(wu)(wu)(wu)(wu)質(zhi)(zhi)稱量(liang)(liang)，逐(zhu)一(yi)(yi)(yi)溶解(jie)(jie)，混(hun)(hun)合(he)，配(pei)(pei)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)。也(ye)可(ke)(ke)(ke)(ke)(ke)(ke)先配(pei)(pei)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)母(mu)液(ye)(ye)(ye)(ye)，使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)時(shi)(shi)(shi)(shi)(shi)按(an)比(bi)(bi)例(li)加(jia)(jia)入(ru)一(yi)(yi)(yi)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)即可(ke)(ke)(ke)(ke)(ke)(ke)。(三)接種(zhong)(zhong)(zhong)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)配(pei)(pei)好后(hou)(hou)(hou)(hou)，應(ying)(ying)(ying)立(li)即進(jin)行(xing)接種(zhong)(zhong)(zhong)。光(guang)合(he)細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)(chan)(chan)(chan)性培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)按(an)種(zhong)(zhong)(zhong)量(liang)(liang)比(bi)(bi)較高(gao)(gao)，一(yi)(yi)(yi)般為(wei)(wei)20%~50%，即菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)母(mu)液(ye)(ye)(ye)(ye)量(liang)(liang)和(he)(he)(he)(he)新配(pei)(pei)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)液(ye)(ye)(ye)(ye)雖之比(bi)(bi)為(wei)(wei)1∶4~1∶1，不(bu)(bu)(bu)(bu)(bu)應(ying)(ying)(ying)低(di)于(yu)(yu)20%，尤其是(shi)(shi)(shi)(shi)(shi)微氣(qi)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)，接種(zhong)(zhong)(zhong)量(liang)(liang)更(geng)應(ying)(ying)(ying)高(gao)(gao)些(xie)(xie)(xie)，否(fou)則(ze)(ze)光(guang)合(he)細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)在(zai)(zai)(zai)(zai)(zai)(zai)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)液(ye)(ye)(ye)(ye)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)很難(nan)占＊＊優勢，影響培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)＊終(zhong)產(chan)(chan)(chan)(chan)(chan)(chan)量(liang)(liang)和(he)(he)(he)(he)質(zhi)(zhi)量(liang)(liang)。(四)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)管(guan)(guan)(guan)理(li)(li)光(guang)合(he)細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)過(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，管(guan)(guan)(guan)理(li)(li)工作(zuo)包括(kuo)日(ri)常(chang)(chang)(chang)管(guan)(guan)(guan)理(li)(li)操(cao)作(zuo)和(he)(he)(he)(he)測(ce)試，生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)情(qing)況(kuang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)觀(guan)察(cha)、檢查以(yi)(yi)(yi)(yi)及(ji)(ji)出現(xian)(xian)問題的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)(fen)(fen)(fen)(fen)析處(chu)(chu)理(li)(li)等(deng)(deng)三個(ge)方(fang)(fang)面。
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