抗(kang)(kang)(kang)凝(ning)(ning)(ning)狗(gou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)（無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun) pet瓶(ping)(ping)(ping)(ping)裝(zhuang)(zhuang)）產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)規(gui)格：100mL滅(mie)(mie)菌(jun)(jun)(jun)說明(ming)：無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)包裝(zhuang)(zhuang)單位(wei)：PET瓶(ping)(ping)(ping)(ping)裝(zhuang)(zhuang)儲存(cun)(cun)條件：2-8℃避光(guang)保(bao)存(cun)(cun)，防止(zhi)冷凍(dong)(dong)(dong)(dong)(dong)。有(you)(you)效(xiao)期：30天適用(yong)(yong)范圍：適應(ying)于(yu)(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)平板生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)、紅(hong)細(xi)(xi)(xi)胞(bao)(bao)提(ti)(ti)取(qu)(qu)、細(xi)(xi)(xi)菌(jun)(jun)(jun)培(pei)(pei)養(yang)(yang)(yang)(yang)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)醫學(xue)研(yan)究基(ji)礎(chu)等(deng)(deng)(deng)(deng)(deng)(deng)。制(zhi)(zhi)(zhi)(zhi)作(zuo)(zuo)工藝：無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)采(cai)(cai)(cai)制(zhi)(zhi)(zhi)(zhi)健(jian)康(kang)狗(gou)動(dong)(dong)脈血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)為原(yuan)(yuan)料，經無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)采(cai)(cai)(cai)集(ji)、檸檬(meng)酸(suan)鈉(na)抗(kang)(kang)(kang)凝(ning)(ning)(ning)、加工、分(fen)(fen)裝(zhuang)(zhuang)而成(cheng)(cheng)(cheng)。使(shi)(shi)(shi)用(yong)(yong)方(fang)法(fa)(fa)：1. 本(ben)(ben)制(zhi)(zhi)(zhi)(zhi)品(pin)(pin)(pin)(pin)需(xu)要(yao)現(xian)(xian)(xian)(xian)采(cai)(cai)(cai)集(ji)，并(bing)經過(guo)(guo)(guo)(guo)(guo)無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)檸檬(meng)酸(suan)鈉(na)抗(kang)(kang)(kang)凝(ning)(ning)(ning)處(chu)理(li)。如(ru)(ru)(ru)需(xu)不(bu)(bu)(bu)(bu)同抗(kang)(kang)(kang)凝(ning)(ning)(ning)劑(ji)，請(qing)提(ti)(ti)前(qian)咨詢(xun)我(wo)司。2. 本(ben)(ben)制(zhi)(zhi)(zhi)(zhi)品(pin)(pin)(pin)(pin)整體(ti)呈現(xian)(xian)(xian)(xian)紅(hong)色(se)至(zhi)暗(an)紅(hong)色(se)，長(chang)時(shi)(shi)間(jian)(jian)靜置，會(hui)有(you)(you)細(xi)(xi)(xi)胞(bao)(bao)沉(chen)(chen)降在(zai)(zai)(zai)(zai)底部(bu)(bu)。3. 收到(dao)本(ben)(ben)制(zhi)(zhi)(zhi)(zhi)品(pin)(pin)(pin)(pin)后(hou)(hou)(hou)，請(qing)放置于(yu)(yu)(yu)(yu)4℃冰(bing)箱保(bao)存(cun)(cun)，嚴(yan)禁凍(dong)(dong)(dong)(dong)(dong)存(cun)(cun)。3. 本(ben)(ben)制(zhi)(zhi)(zhi)(zhi)品(pin)(pin)(pin)(pin)為現(xian)(xian)(xian)(xian)采(cai)(cai)(cai)現(xian)(xian)(xian)(xian)用(yong)(yong)，保(bao)質(zhi)期30天，收到(dao)后(hou)(hou)(hou)請(qing)及時(shi)(shi)使(shi)(shi)(shi)用(yong)(yong)，建(jian)(jian)議2周內(nei)(nei)(nei)用(yong)(yong)完。4. 本(ben)(ben)制(zhi)(zhi)(zhi)(zhi)品(pin)(pin)(pin)(pin)易(yi)產(chan)(chan)(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)溶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)現(xian)(xian)(xian)(xian)象，每天需(xu)輕(qing)輕(qing)將(jiang)紅(hong)細(xi)(xi)(xi)胞(bao)(bao)全(quan)部(bu)(bu)搖(yao)起(qi)(qi)。5. 收到(dao)本(ben)(ben)制(zhi)(zhi)(zhi)(zhi)品(pin)(pin)(pin)(pin)后(hou)(hou)(hou)如(ru)(ru)(ru)有(you)(you)性質(zhi)不(bu)(bu)(bu)(bu)符，請(qing)于(yu)(yu)(yu)(yu)3日內(nei)(nei)(nei)與我(wo)司聯(lian)系(xi)，以(yi)(yi)(yi)便于(yu)(yu)(yu)(yu)我(wo)司今(jin)早給出(chu)(chu)判定(ding)答復。3日后(hou)(hou)(hou)與我(wo)司聯(lian)系(xi)，我(wo)司有(you)(you)權判定(ding)非產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)質(zhi)量(liang)(liang)(liang)問題(ti)。注意事項：1. 本(ben)(ben)制(zhi)(zhi)(zhi)(zhi)品(pin)(pin)(pin)(pin)請(qing)搖(yao)均勻(yun)后(hou)(hou)(hou)，直(zhi)(zhi)接(jie)使(shi)(shi)(shi)用(yong)(yong)即(ji)可(ke)(ke)。請(qing)勿(wu)(wu)(wu)將(jiang)使(shi)(shi)(shi)用(yong)(yong)后(hou)(hou)(hou)剩余的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)劑(ji)再(zai)(zai)倒入(ru)(ru)(ru)原(yuan)(yuan)包裝(zhuang)(zhuang)瓶(ping)(ping)(ping)(ping)內(nei)(nei)(nei)。2. 本(ben)(ben)制(zhi)(zhi)(zhi)(zhi)品(pin)(pin)(pin)(pin)使(shi)(shi)(shi)用(yong)(yong)過(guo)(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)，注意防止(zhi)污染(ran)，在(zai)(zai)(zai)(zai)室(shi)溫(wen)(wen)(wen)內(nei)(nei)(nei)放置過(guo)(guo)(guo)(guo)(guo)久(jiu)(jiu)(jiu)會(hui)影(ying)響使(shi)(shi)(shi)用(yong)(yong)效(xiao)果，使(shi)(shi)(shi)用(yong)(yong)后(hou)(hou)(hou)請(qing)盡(jin)快(kuai)放入(ru)(ru)(ru)2-8℃冰(bing)箱保(bao)存(cun)(cun)。3. 為了(le)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)健(jian)康(kang)與安(an)全(quan)，請(qing)穿實驗(yan)服并(bing)佩戴一(yi)(yi)(yi)(yi)(yi)次性手套進(jin)行操(cao)作(zuo)(zuo)。4. 本(ben)(ben)制(zhi)(zhi)(zhi)(zhi)品(pin)(pin)(pin)(pin)僅(jin)供(gong)科研(yan)使(shi)(shi)(shi)用(yong)(yong)，嚴(yan)禁用(yong)(yong)于(yu)(yu)(yu)(yu)臨床診(zhen)斷(duan)和(he)(he)藥物(wu)(wu)(wu)(wu)(wu)等(deng)(deng)(deng)(deng)(deng)(deng)用(yong)(yong)途。 動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)，標準(zhun)品(pin)(pin)(pin)(pin)對(dui)照(zhao)品(pin)(pin)(pin)(pin)，化(hua)(hua)(hua)(hua)學(xue)試(shi)(shi)劑(ji)，酶(mei)(mei)(mei)聯(lian)免疫試(shi)(shi)劑(ji)盒，白(bai)(bai)(bai)(bai)(bai)(bai)介素(su)(su)試(shi)(shi)劑(ji)盒，金(jin)標檢測試(shi)(shi)劑(ji)盒，微生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)，蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)，ELISA種(zhong)屬涵蓋廣，憑借(jie)多(duo)年行業經驗(yan)，完善的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)售后(hou)(hou)(hou)服務(wu)，高(gao)質(zhi)量(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)。歡迎來電咨詢(xun)。 特(te)點：三次無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)過(guo)(guo)(guo)(guo)(guo)濾(lv)，輻射滅(mie)(mie)菌(jun)(jun)(jun)，批(pi)次新，保(bao)質(zhi)期久(jiu)(jiu)(jiu)，貼(tie)壁效(xiao)果好(hao)，增(zeng)(zeng)殖效(xiao)率(lv)高(gao)。產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)用(yong)(yong)途：●提(ti)(ti)供(gong)基(ji)本(ben)(ben)營養(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)質(zhi)：氨基(ji)酸(suan)、維(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素(su)(su)、無(wu)(wu)(wu)(wu)(wu)機(ji)物(wu)(wu)(wu)(wu)(wu)、脂(zhi)類物(wu)(wu)(wu)(wu)(wu)質(zhi)、核(he)酸(suan)衍(yan)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)等(deng)(deng)(deng)(deng)(deng)(deng)，是(shi)(shi)(shi)細(xi)(xi)(xi)胞(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)必須(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)質(zhi)。●提(ti)(ti)供(gong)激(ji)(ji)素(su)(su)和(he)(he)各(ge)種(zhong)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)(zi)：胰(yi)(yi)島素(su)(su)、腎上腺皮質(zhi)激(ji)(ji)素(su)(su)（地塞(sai)米松）、類固(gu)醇激(ji)(ji)素(su)(su)（雌二醇、睪酮、孕酮）等(deng)(deng)(deng)(deng)(deng)(deng)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)(zi)如(ru)(ru)(ru)成(cheng)(cheng)(cheng)纖(xian)(xian)維(wei)細(xi)(xi)(xi)胞(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)(zi)、表皮生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)(zi)、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小板生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)(zi)等(deng)(deng)(deng)(deng)(deng)(deng)。●提(ti)(ti)供(gong)結合(he)(he)(he)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)：結合(he)(he)(he)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)作(zuo)(zuo)用(yong)(yong)是(shi)(shi)(shi)攜(xie)帶(dai)(dai)重(zhong)要(yao)地低(di)分(fen)(fen)子(zi)(zi)量(liang)(liang)(liang)物(wu)(wu)(wu)(wu)(wu)質(zhi)，如(ru)(ru)(ru)白(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)攜(xie)帶(dai)(dai)維(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素(su)(su)、脂(zhi)肪(fang)、以(yi)(yi)(yi)及激(ji)(ji)素(su)(su)等(deng)(deng)(deng)(deng)(deng)(deng)，轉鐵蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)攜(xie)帶(dai)(dai)鐵。結合(he)(he)(he)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)在(zai)(zai)(zai)(zai)細(xi)(xi)(xi)胞(bao)(bao)代謝過(guo)(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)起(qi)(qi)重(zhong)要(yao)作(zuo)(zuo)用(yong)(yong)。●提(ti)(ti)供(gong)促(cu)接(jie)觸和(he)(he)伸(shen)展因(yin)(yin)(yin)子(zi)(zi)使(shi)(shi)(shi)細(xi)(xi)(xi)胞(bao)(bao)貼(tie)壁免受(shou)(shou)機(ji)械損(sun)傷。●對(dui)培(pei)(pei)養(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)胞(bao)(bao)起(qi)(qi)到(dao)某些(xie)(xie)保(bao)護作(zuo)(zuo)用(yong)(yong)：有(you)(you)一(yi)(yi)(yi)(yi)(yi)些(xie)(xie)細(xi)(xi)(xi)胞(bao)(bao)，如(ru)(ru)(ru)內(nei)(nei)(nei)皮細(xi)(xi)(xi)胞(bao)(bao)、骨髓樣細(xi)(xi)(xi)胞(bao)(bao)可(ke)(ke)以(yi)(yi)(yi)釋放蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)(mei)(mei)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)含(han)有(you)(you)抗(kang)(kang)(kang)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)(mei)(mei)成(cheng)(cheng)(cheng)分(fen)(fen)，起(qi)(qi)到(dao)中(zhong)(zhong)(zhong)和(he)(he)作(zuo)(zuo)用(yong)(yong)。這(zhe)(zhe)種(zhong)作(zuo)(zuo)用(yong)(yong)是(shi)(shi)(shi)偶然(ran)發(fa)(fa)(fa)現(xian)(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，現(xian)(xian)(xian)(xian)在(zai)(zai)(zai)(zai)則(ze)有(you)(you)目的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)使(shi)(shi)(shi)用(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)來終止(zhi)胰(yi)(yi)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)(mei)(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消(xiao)化(hua)(hua)(hua)(hua)作(zuo)(zuo)用(yong)(yong)。因(yin)(yin)(yin)為胰(yi)(yi)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)(mei)(mei)已(yi)經被(bei)廣泛用(yong)(yong)于(yu)(yu)(yu)(yu)貼(tie)壁細(xi)(xi)(xi)胞(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消(xiao)化(hua)(hua)(hua)(hua)傳(chuan)代。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)形成(cheng)(cheng)(cheng)了(le)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)粘(zhan)度(du)，可(ke)(ke)以(yi)(yi)(yi)保(bao)護細(xi)(xi)(xi)胞(bao)(bao)免受(shou)(shou)機(ji)械損(sun)傷，特(te)別是(shi)(shi)(shi)在(zai)(zai)(zai)(zai)懸(xuan)(xuan)浮(fu)(fu)培(pei)(pei)養(yang)(yang)(yang)(yang)攪拌時(shi)(shi)，粘(zhan)度(du)起(qi)(qi)到(dao)重(zhong)要(yao)作(zuo)(zuo)用(yong)(yong)。抗(kang)(kang)(kang)凝(ning)(ning)(ning)狗(gou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)（無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun) pet瓶(ping)(ping)(ping)(ping)裝(zhuang)(zhuang)）主要(yao)成(cheng)(cheng)(cheng)分(fen)(fen) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)由(you)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)去(qu)除纖(xian)(xian)維(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)而形成(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)種(zhong)很復雜的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)(he)(he)物(wu)(wu)(wu)(wu)(wu)，其(qi)組成(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)份雖大(da)部(bu)(bu)分(fen)(fen)已(yi)知，但還有(you)(you)一(yi)(yi)(yi)(yi)(yi)部(bu)(bu)分(fen)(fen)尚不(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，且(qie)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組成(cheng)(cheng)(cheng)及含(han)量(liang)(liang)(liang)常(chang)(chang)隨(sui)(sui)供(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性別、年齡、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)條件和(he)(he)營養(yang)(yang)(yang)(yang)條件不(bu)(bu)(bu)(bu)同而異(yi)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)中(zhong)(zhong)(zhong)不(bu)(bu)(bu)(bu)含(han)纖(xian)(xian)維(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠(jiao)狀液體(ti)，具有(you)(you)維(wei)持血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正常(chang)(chang)粘(zhan)度(du)、酸(suan)堿度(du)、滲透壓(ya)等(deng)(deng)(deng)(deng)(deng)(deng)作(zuo)(zuo)用(yong)(yong)。 它(ta)主要(yao)由(you)水和(he)(he)各(ge)種(zhong)化(hua)(hua)(hua)(hua)學(xue)成(cheng)(cheng)(cheng)分(fen)(fen)組成(cheng)(cheng)(cheng)，這(zhe)(zhe)些(xie)(xie)化(hua)(hua)(hua)(hua)學(xue)成(cheng)(cheng)(cheng)分(fen)(fen)包括(kuo)白(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)、α1、α2、β、γ-球蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)，甘(gan)油三酯，總(zong)膽固(gu)醇，谷丙(bing)轉氨酶(mei)(mei)(mei)等(deng)(deng)(deng)(deng)(deng)(deng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)含(han)有(you)(you)各(ge)種(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)、多(duo)肽、脂(zhi)肪(fang)、碳水化(hua)(hua)(hua)(hua)合(he)(he)(he)物(wu)(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)(zi)、激(ji)(ji)素(su)(su)、無(wu)(wu)(wu)(wu)(wu)機(ji)物(wu)(wu)(wu)(wu)(wu)等(deng)(deng)(deng)(deng)(deng)(deng)，這(zhe)(zhe)些(xie)(xie)物(wu)(wu)(wu)(wu)(wu)質(zhi)對(dui)促(cu)進(jin)細(xi)(xi)(xi)胞(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)或抑制(zhi)(zhi)(zhi)(zhi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)活(huo)性是(shi)(shi)(shi)達(da)到(dao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)平衡(heng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。鑒別方(fang)式 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液凝(ning)(ning)(ning)固(gu)析(xi)出(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)淡(dan)黃(huang)色(se)透明(ming)液體(ti)。如(ru)(ru)(ru)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液自(zi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管內(nei)(nei)(nei)抽出(chu)(chu)，放入(ru)(ru)(ru)試(shi)(shi)管中(zhong)(zhong)(zhong)，不(bu)(bu)(bu)(bu)加抗(kang)(kang)(kang)凝(ning)(ning)(ning)劑(ji)，則(ze)凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)(fan)應(ying)被(bei)激(ji)(ji)活(huo)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液迅(xun)速凝(ning)(ning)(ning)固(gu)，形成(cheng)(cheng)(cheng)膠(jiao)凍(dong)(dong)(dong)(dong)(dong)。凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)塊收縮，其(qi)周圍所析(xi)出(chu)(chu)之(zhi)(zhi)淡(dan)黃(huang)色(se)透明(ming)液體(ti)即(ji)為血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，也(ye)可(ke)(ke)于(yu)(yu)(yu)(yu)凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后(hou)(hou)(hou)經離(li)心取(qu)(qu)得。在(zai)(zai)(zai)(zai)凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)過(guo)(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)，纖(xian)(xian)維(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)轉變(bian)成(cheng)(cheng)(cheng)纖(xian)(xian)維(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)塊，所以(yi)(yi)(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)無(wu)(wu)(wu)(wu)(wu)纖(xian)(xian)維(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)，這(zhe)(zhe)一(yi)(yi)(yi)(yi)(yi)點是(shi)(shi)(shi)與血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別。而在(zai)(zai)(zai)(zai)凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)(fan)應(ying)中(zhong)(zhong)(zhong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小板釋放出(chu)(chu)許多(duo)物(wu)(wu)(wu)(wu)(wu)質(zhi)，各(ge)凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)因(yin)(yin)(yin)子(zi)(zi)也(ye)都(dou)發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)了(le)變(bian)化(hua)(hua)(hua)(hua)。 這(zhe)(zhe)些(xie)(xie)成(cheng)(cheng)(cheng)分(fen)(fen)都(dou)留(liu)在(zai)(zai)(zai)(zai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)并(bing)繼續發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)變(bian)化(hua)(hua)(hua)(hua)，如(ru)(ru)(ru)凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)酶(mei)(mei)(mei)原(yuan)(yuan)變(bian)成(cheng)(cheng)(cheng)凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)酶(mei)(mei)(mei)，并(bing)隨(sui)(sui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)存(cun)(cun)放時(shi)(shi)間(jian)(jian)逐漸減少(shao)以(yi)(yi)(yi)至(zhi)消(xiao)失。這(zhe)(zhe)些(xie)(xie)也(ye)都(dou)是(shi)(shi)(shi)與血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)區別之(zhi)(zhi)處(chu)。但大(da)量(liang)(liang)(liang)未(wei)參加凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)(fan)應(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)質(zhi)則(ze)與血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)基(ji)本(ben)(ben)相(xiang)同。為避免抗(kang)(kang)(kang)凝(ning)(ning)(ning)劑(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)干擾，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液中(zhong)(zhong)(zhong)許多(duo)化(hua)(hua)(hua)(hua)學(xue)成(cheng)(cheng)(cheng)分(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)析(xi)，都(dou)以(yi)(yi)(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為樣品(pin)(pin)(pin)(pin)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)質(zhi)期：1.長(chang)期保(bao)存(cun)(cun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必須(xu)(xu)儲存(cun)(cun)于(yu)(yu)(yu)(yu)-20℃ - 70℃低(di)溫(wen)(wen)(wen)冰(bing)箱中(zhong)(zhong)(zhong)。4℃冰(bing)箱中(zhong)(zhong)(zhong)保(bao)存(cun)(cun)時(shi)(shi)間(jian)(jian)切勿(wu)(wu)(wu)超(chao)過(guo)(guo)(guo)(guo)(guo)1個(ge)月(yue)。由(you)于(yu)(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結冰(bing)時(shi)(shi)體(ti)積會(hui)增(zeng)(zeng)加約10%,因(yin)(yin)(yin)此(ci)(ci)(ci)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)凍(dong)(dong)(dong)(dong)(dong)入(ru)(ru)(ru)低(di)溫(wen)(wen)(wen)冰(bing)箱前(qian)，必須(xu)(xu)預留(liu)一(yi)(yi)(yi)(yi)(yi)定(ding)體(ti)積空(kong)間(jian)(jian)，否則(ze)易(yi)發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)污染(ran)或玻璃瓶(ping)(ping)(ping)(ping)凍(dong)(dong)(dong)(dong)(dong)裂。2.一(yi)(yi)(yi)(yi)(yi)般廠商提(ti)(ti)供(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)，無(wu)(wu)(wu)(wu)(wu)需(xu)再(zai)(zai)過(guo)(guo)(guo)(guo)(guo)濾(lv)除菌(jun)(jun)(jun)。如(ru)(ru)(ru)發(fa)(fa)(fa)現(xian)(xian)(xian)(xian)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)有(you)(you)懸(xuan)(xuan)浮(fu)(fu)物(wu)(wu)(wu)(wu)(wu)，則(ze)可(ke)(ke)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)加入(ru)(ru)(ru)培(pei)(pei)養(yang)(yang)(yang)(yang)液內(nei)(nei)(nei)一(yi)(yi)(yi)(yi)(yi)起(qi)(qi)過(guo)(guo)(guo)(guo)(guo)濾(lv)，切勿(wu)(wu)(wu)直(zhi)(zhi)接(jie)過(guo)(guo)(guo)(guo)(guo)濾(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。3.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)： 瓶(ping)(ping)(ping)(ping)裝(zhuang)(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)需(xu)采(cai)(cai)(cai)用(yong)(yong)逐步(bu)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)法(fa)(fa)：-20℃ 至(zhi) -70℃ 低(di)溫(wen)(wen)(wen)冰(bing)箱中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)放入(ru)(ru)(ru)4℃冰(bing)箱中(zhong)(zhong)(zhong)溶解(jie)(jie)(jie)(jie)(jie)(jie)(jie)1天。然(ran)后(hou)(hou)(hou)移入(ru)(ru)(ru)室(shi)溫(wen)(wen)(wen)，待(dai)全(quan)部(bu)(bu)溶解(jie)(jie)(jie)(jie)(jie)(jie)(jie)后(hou)(hou)(hou)再(zai)(zai)分(fen)(fen)裝(zhuang)(zhuang)。在(zai)(zai)(zai)(zai)溶解(jie)(jie)(jie)(jie)(jie)(jie)(jie)過(guo)(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)需(xu)不(bu)(bu)(bu)(bu)斷(duan)輕(qing)輕(qing)搖(yao)晃(huang)均勻(yun)（小心勿(wu)(wu)(wu)造(zao)成(cheng)(cheng)(cheng)氣泡），使(shi)(shi)(shi)溫(wen)(wen)(wen)度(du)與成(cheng)(cheng)(cheng)分(fen)(fen)均一(yi)(yi)(yi)(yi)(yi)，減少(shao)沉(chen)(chen)淀(dian)(dian)(dian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)。切勿(wu)(wu)(wu)直(zhi)(zhi)接(jie)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從-20℃進(jin)入(ru)(ru)(ru)37℃解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)，這(zhe)(zhe)樣因(yin)(yin)(yin)溫(wen)(wen)(wen)度(du)改變(bian)太(tai)大(da)，容易(yi)造(zao)成(cheng)(cheng)(cheng)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)凝(ning)(ning)(ning)集(ji)而出(chu)(chu)現(xian)(xian)(xian)(xian)沉(chen)(chen)淀(dian)(dian)(dian)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)量(liang)(liang)(liang)標準(zhun) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)量(liang)(liang)(liang)高(gao)低(di)取(qu)(qu)決(jue)于(yu)(yu)(yu)(yu)兩(liang)方(fang)面因(yin)(yin)(yin)素(su)(su)：一(yi)(yi)(yi)(yi)(yi)是(shi)(shi)(shi)取(qu)(qu)材(cai)對(dui)象，二是(shi)(shi)(shi)取(qu)(qu)材(cai)過(guo)(guo)(guo)(guo)(guo)程(cheng)。用(yong)(yong)于(yu)(yu)(yu)(yu)取(qu)(qu)材(cai)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)應(ying)健(jian)康(kang)無(wu)(wu)(wu)(wu)(wu)病并(bing)且(qie)在(zai)(zai)(zai)(zai)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)出(chu)(chu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)天數(shu)之(zhi)(zhi)內(nei)(nei)(nei)，取(qu)(qu)材(cai)過(guo)(guo)(guo)(guo)(guo)程(cheng)應(ying)嚴(yan)格按(an)照(zhao)操(cao)作(zuo)(zuo)規(gui)程(cheng)執行，制(zhi)(zhi)(zhi)(zhi)備出(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)要(yao)經過(guo)(guo)(guo)(guo)(guo)嚴(yan)格的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)量(liang)(liang)(liang)鑒定(ding)。常(chang)(chang)見問題(ti)保(bao)存(cun)(cun)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)法(fa)(fa)？ 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)應(ying)保(bao)存(cun)(cun)在(zai)(zai)(zai)(zai)-5℃至(zhi)-2O℃若(ruo)(ruo)存(cun)(cun)放于(yu)(yu)(yu)(yu)4℃時(shi)(shi)，請(qing)勿(wu)(wu)(wu)超(chao)過(guo)(guo)(guo)(guo)(guo)一(yi)(yi)(yi)(yi)(yi)個(ge)月(yue)。若(ruo)(ruo)您一(yi)(yi)(yi)(yi)(yi)次無(wu)(wu)(wu)(wu)(wu)法(fa)(fa)用(yong)(yong)完一(yi)(yi)(yi)(yi)(yi)瓶(ping)(ping)(ping)(ping)，建(jian)(jian)議將(jiang)無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)分(fen)(fen)裝(zhuang)(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)至(zhi)恰(qia)當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)滅(mie)(mie)菌(jun)(jun)(jun)容器內(nei)(nei)(nei)，再(zai)(zai)放回冷凍(dong)(dong)(dong)(dong)(dong)。如(ru)(ru)(ru)何解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)才不(bu)(bu)(bu)(bu)會(hui)使(shi)(shi)(shi)產(chan)(chan)(chan)品(pin)(pin)(pin)(pin)質(zhi)量(liang)(liang)(liang)受(shou)(shou)損(sun)？ 將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從冷凍(dong)(dong)(dong)(dong)(dong)箱取(qu)(qu)出(chu)(chu)后(hou)(hou)(hou)，先置于(yu)(yu)(yu)(yu)2-8℃冰(bing)箱使(shi)(shi)(shi)之(zhi)(zhi)溶解(jie)(jie)(jie)(jie)(jie)(jie)(jie)，然(ran)后(hou)(hou)(hou)在(zai)(zai)(zai)(zai)室(shi)溫(wen)(wen)(wen)下(xia)使(shi)(shi)(shi)之(zhi)(zhi)全(quan)溶。但必須(xu)(xu)注意的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)，溶解(jie)(jie)(jie)(jie)(jie)(jie)(jie)過(guo)(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)必須(xu)(xu)規(gui)則(ze)地搖(yao)晃(huang)均勻(yun)。如(ru)(ru)(ru)何避免沉(chen)(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)(chan)(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)？1.解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)，請(qing)按(an)照(zhao)所建(jian)(jian)議的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)逐步(bu)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)法(fa)(fa)（-2O℃至(zhi)4℃至(zhi)室(shi)溫(wen)(wen)(wen)），若(ruo)(ruo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)時(shi)(shi)改變(bian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)溫(wen)(wen)(wen)度(du)太(tai)大(da)（如(ru)(ru)(ru)-20℃至(zhi)37℃），實驗(yan)顯(xian)示非常(chang)(chang)容易(yi)產(chan)(chan)(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)沉(chen)(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)(wu)。2.解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)，請(qing)隨(sui)(sui)時(shi)(shi)將(jiang)之(zhi)(zhi)搖(yao)晃(huang)均勻(yun)，使(shi)(shi)(shi)溫(wen)(wen)(wen)度(du)及成(cheng)(cheng)(cheng)分(fen)(fen)均一(yi)(yi)(yi)(yi)(yi)，減少(shao)沉(chen)(chen)淀(dian)(dian)(dian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)3.請(qing)勿(wu)(wu)(wu)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)置于(yu)(yu)(yu)(yu)37℃太(tai)久(jiu)(jiu)(jiu)。若(ruo)(ruo)在(zai)(zai)(zai)(zai)37℃放置太(tai)久(jiu)(jiu)(jiu)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)會(hui)變(bian)得混濁，同時(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)許多(duo)較不(bu)(bu)(bu)(bu)穩定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)分(fen)(fen)也(ye)會(hui)因(yin)(yin)(yin)此(ci)(ci)(ci)受(shou)(shou)到(dao)損(sun)害，而影(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)量(liang)(liang)(liang)。4.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱滅(mie)(mie)活(huo)非常(chang)(chang)容易(yi)造(zao)成(cheng)(cheng)(cheng)沉(chen)(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)增(zeng)(zeng)多(duo)，若(ruo)(ruo)非必要(yao)，可(ke)(ke)以(yi)(yi)(yi)無(wu)(wu)(wu)(wu)(wu)須(xu)(xu)做(zuo)此(ci)(ci)(ci)步(bu)驟。5.若(ruo)(ruo)必須(xu)(xu)做(zuo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱滅(mie)(mie)活(huo)，請(qing)遵守56℃，30分(fen)(fen)鐘(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)(yuan)則(ze)，并(bing)且(qie)隨(sui)(sui)時(shi)(shi)搖(yao)晃(huang)均勻(yun)。溫(wen)(wen)(wen)度(du)過(guo)(guo)(guo)(guo)(guo)高(gao)，時(shi)(shi)間(jian)(jian)過(guo)(guo)(guo)(guo)(guo)久(jiu)(jiu)(jiu)或搖(yao)晃(huang)不(bu)(bu)(bu)(bu)均勻(yun)，都(dou)會(hui)造(zao)成(cheng)(cheng)(cheng)沉(chen)(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)增(zeng)(zeng)多(duo)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)后(hou)(hou)(hou)發(fa)(fa)(fa)現(xian)(xian)(xian)(xian)有(you)(you)絮(xu)狀沉(chen)(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)(wu)出(chu)(chu)現(xian)(xian)(xian)(xian)，該如(ru)(ru)(ru)何處(chu)理(li)？ 欲(yu)去(qu)除這(zhe)(zhe)些(xie)(xie)絮(xu)狀沉(chen)(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)(wu)，可(ke)(ke)以(yi)(yi)(yi)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)分(fen)(fen)裝(zhuang)(zhuang)至(zhi)無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)離(li)心管內(nei)(nei)(nei)，以(yi)(yi)(yi)400g稍微離(li)心，上清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液即(ji)可(ke)(ke)接(jie)著(zhu)加入(ru)(ru)(ru)培(pei)(pei)養(yang)(yang)(yang)(yang)基(ji)內(nei)(nei)(nei)一(yi)(yi)(yi)(yi)(yi)起(qi)(qi)過(guo)(guo)(guo)(guo)(guo)濾(lv)。但不(bu)(bu)(bu)(bu)建(jian)(jian)議以(yi)(yi)(yi)過(guo)(guo)(guo)(guo)(guo)濾(lv)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)法(fa)(fa)去(qu)除這(zhe)(zhe)些(xie)(xie)絮(xu)狀沉(chen)(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)(wu)，因(yin)(yin)(yin)為它(ta)可(ke)(ke)能會(hui)阻塞(sai)過(guo)(guo)(guo)(guo)(guo)濾(lv)膜。
抗(kang)(kang)凝(ning)兔(tu)(tu)血(xue)(xue)(xue)(xue)(xue)(xue)(無(wu)(wu)(wu)菌 pet瓶裝(zhuang)(zhuang)(zhuang))【產(chan)(chan)品(pin)規(gui)格】Pet瓶裝(zhuang)(zhuang)(zhuang)【產(chan)(chan)品(pin)用(yong)(yong)(yong)(yong)(yong)途(tu)】適應(ying)(ying)(ying)于(yu)(yu)(yu)細(xi)(xi)胞(bao)(bao)(bao)培(pei)(pei)養(yang)(yang)、免(mian)(mian)疫檢(jian)(jian)測(ce)等(deng)(deng)(deng)。【操(cao)作(zuo)(zuo)(zuo)(zuo)流(liu)程(cheng)(cheng)】請(qing)(qing)搖(yao)均(jun)勻(yun)后(hou)(hou)(hou)(hou)直(zhi)接(jie)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。【儲存(cun)(cun)(cun)(cun)條件及(ji)(ji)有效(xiao)期(qi)(qi)(qi)】 1、密封(feng)保(bao)(bao)存(cun)(cun)(cun)(cun)于(yu)(yu)(yu)-10℃～-30℃ 2、有效(xiao)期(qi)(qi)(qi)五年(nian)【注意(yi)(yi)事(shi)(shi)項(xiang)】 1、本產(chan)(chan)品(pin)僅供科研使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。 2、產(chan)(chan)品(pin)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)，注意(yi)(yi)防止污(wu)染，在(zai)(zai)室(shi)(shi)溫(wen)(wen)(wen)(wen)內(nei)(nei)(nei)放(fang)置過(guo)(guo)(guo)(guo)(guo)久會(hui)影響(xiang)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)效(xiao)果，使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)(hou)請(qing)(qing)盡(jin)快(kuai)放(fang)入(ru)(ru)-10℃～-30℃保(bao)(bao)存(cun)(cun)(cun)(cun)。 3、請(qing)(qing)勿(wu)將使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)后(hou)(hou)(hou)(hou)剩余的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)劑(ji)再(zai)(zai)倒入(ru)(ru)原(yuan)包裝(zhuang)(zhuang)(zhuang)瓶內(nei)(nei)(nei)。【生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)日期(qi)(qi)(qi)】詳(xiang)見標簽(qian)，請(qing)(qing)在(zai)(zai)產(chan)(chan)品(pin)標簽(qian)標注的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有效(xiao)期(qi)(qi)(qi)內(nei)(nei)(nei)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。 抗(kang)(kang)凝(ning)兔(tu)(tu)血(xue)(xue)(xue)(xue)(xue)(xue)(無(wu)(wu)(wu)菌 pet瓶裝(zhuang)(zhuang)(zhuang))在(zai)(zai)動(dong)物(wu)(wu)(wu)細(xi)(xi)胞(bao)(bao)(bao)培(pei)(pei)養(yang)(yang)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)：①提(ti)(ti)供細(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)存(cun)(cun)(cun)(cun)和(he)增(zeng)(zeng)(zeng)殖所必(bi)(bi)(bi)需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)調節因(yin)(yin)(yin)子(zi)。②補充(chong)基礎培(pei)(pei)養(yang)(yang)基中(zhong)(zhong)沒有或量(liang)不(bu)足的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)營(ying)養(yang)(yang)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)。③含(han)(han)有一(yi)些(xie)可(ke)(ke)(ke)供貼壁(bi)細(xi)(xi)胞(bao)(bao)(bao)型細(xi)(xi)胞(bao)(bao)(bao)貼壁(bi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基質(zhi)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)。④提(ti)(ti)供載體蛋白(bai)(bai)(bai)(bai)，可(ke)(ke)(ke)結(jie)合(he)維(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素、脂(zhi)質(zhi)、金屬離子(zi)等(deng)(deng)(deng)。⑤在(zai)(zai)一(yi)些(xie)情(qing)況(kuang)下，中(zhong)(zhong)和(he)毒性(xing)(xing)物(wu)(wu)(wu)質(zhi)保(bao)(bao)護(hu)細(xi)(xi)胞(bao)(bao)(bao)不(bu)受傷(shang)害。⑥給(gei)培(pei)(pei)養(yang)(yang)液(ye)提(ti)(ti)供良好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)緩沖系(xi)統。⑦提(ti)(ti)供蛋白(bai)(bai)(bai)(bai)酶抑制(zhi)(zhi)劑(ji)，保(bao)(bao)護(hu)細(xi)(xi)胞(bao)(bao)(bao)免(mian)(mian)受死細(xi)(xi)胞(bao)(bao)(bao)釋放(fang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)蛋白(bai)(bai)(bai)(bai)酶的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)傷(shang)害。 質(zhi)量(liang)標準 內(nei)(nei)(nei)毒素含(han)(han)量(liang)低(di)(di)于(yu)(yu)(yu)5EU/ml，克(ke)隆形(xing)成(cheng)(cheng)(cheng)(cheng)率≥90%。適用(yong)(yong)(yong)(yong)(yong)范圍(wei)：用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)細(xi)(xi)胞(bao)(bao)(bao)株的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)藏及(ji)(ji)嬌(jiao)貴細(xi)(xi)胞(bao)(bao)(bao)株培(pei)(pei)養(yang)(yang)。更多(duo)優勢血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)產(chan)(chan)品(pin)：血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)存(cun)(cun)(cun)(cun)方(fang)法： (1)長(chang)(chang)(chang)期(qi)(qi)(qi)保(bao)(bao)存(cun)(cun)(cun)(cun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)必(bi)(bi)(bi)須儲存(cun)(cun)(cun)(cun)于(yu)(yu)(yu)-20℃ - 70℃ 低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)(bing)箱(xiang)(xiang)中(zhong)(zhong).4℃冰(bing)(bing)箱(xiang)(xiang)中(zhong)(zhong)保(bao)(bao)存(cun)(cun)(cun)(cun)時(shi)間切勿(wu)超過(guo)(guo)(guo)(guo)(guo)1個(ge)月.由(you)于(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)結(jie)冰(bing)(bing)時(shi)體積會(hui)增(zeng)(zeng)(zeng)加(jia)約10%,因(yin)(yin)(yin)此(ci)(ci)(ci),血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)在(zai)(zai)凍(dong)(dong)(dong)(dong)(dong)入(ru)(ru)低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)(bing)箱(xiang)(xiang)前(qian),必(bi)(bi)(bi)須預(yu)留一(yi)定(ding)體積空間,否(fou)(fou)則易(yi)發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)污(wu)染或玻璃(li)瓶凍(dong)(dong)(dong)(dong)(dong)裂. (2)一(yi)般(ban)廠(chang)商提(ti)(ti)供的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)為(wei)無(wu)(wu)(wu)菌,無(wu)(wu)(wu)需(xu)(xu)再(zai)(zai)過(guo)(guo)(guo)(guo)(guo)濾(lv)除(chu)(chu)(chu)菌.如(ru)發(fa)(fa)(fa)現(xian)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)有懸浮物(wu)(wu)(wu),則可(ke)(ke)(ke)將血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)加(jia)入(ru)(ru)培(pei)(pei)養(yang)(yang)液(ye)內(nei)(nei)(nei)一(yi)起(qi)(qi)過(guo)(guo)(guo)(guo)(guo)濾(lv),切勿(wu)直(zhi)接(jie)過(guo)(guo)(guo)(guo)(guo)濾(lv)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing). (3)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)： 瓶裝(zhuang)(zhuang)(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)需(xu)(xu)采(cai)用(yong)(yong)(yong)(yong)(yong)逐(zhu)步解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)法:-20℃ 至(zhi)(zhi) -70℃ 低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)(bing)箱(xiang)(xiang)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)放(fang)入(ru)(ru)4℃冰(bing)(bing)箱(xiang)(xiang)中(zhong)(zhong)溶(rong)解(jie)(jie)(jie)(jie)(jie)1天(tian).然(ran)后(hou)(hou)(hou)(hou)移(yi)入(ru)(ru)室(shi)(shi)溫(wen)(wen)(wen)(wen),待全部(bu)溶(rong)解(jie)(jie)(jie)(jie)(jie)后(hou)(hou)(hou)(hou)再(zai)(zai)分(fen)(fen)(fen)(fen)(fen)裝(zhuang)(zhuang)(zhuang).在(zai)(zai)溶(rong)解(jie)(jie)(jie)(jie)(jie)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)需(xu)(xu)不(bu)斷(duan)輕(qing)輕(qing)搖(yao)晃均(jun)勻(yun)(小心勿(wu)造成(cheng)(cheng)(cheng)(cheng)氣(qi)(qi)(qi)泡),使(shi)(shi)溫(wen)(wen)(wen)(wen)度(du)與(yu)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)均(jun)一(yi),減少沉(chen)(chen)淀(dian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng).切勿(wu)直(zhi)接(jie)將血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)從-20℃進(jin)(jin)入(ru)(ru)37℃解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong),這(zhe)樣因(yin)(yin)(yin)溫(wen)(wen)(wen)(wen)度(du)改(gai)變太大(da)(da),容(rong)易(yi)造成(cheng)(cheng)(cheng)(cheng)蛋白(bai)(bai)(bai)(bai)質(zhi)凝(ning)集(ji)而(er)出(chu)現(xian)沉(chen)(chen)淀(dian). (4)熱(re)滅(mie)活是(shi)指56℃, 30分(fen)(fen)(fen)(fen)(fen)鐘加(jia)熱(re)已*解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing).加(jia)熱(re)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)須規(gui)則搖(yao)晃均(jun)勻(yun).此(ci)(ci)(ci)熱(re)處(chu)(chu)(chu)理(li)(li)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)目(mu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)使(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補體成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(complement)滅(mie)活.除(chu)(chu)(chu)非(fei)必(bi)(bi)(bi)須,一(yi)般(ban)不(bu)建議(yi)作(zuo)(zuo)(zuo)(zuo)此(ci)(ci)(ci)熱(re)處(chu)(chu)(chu)理(li)(li),因(yin)(yin)(yin)為(wei)熱(re)處(chu)(chu)(chu)理(li)(li)會(hui)造成(cheng)(cheng)(cheng)(cheng)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)沉(chen)(chen)淀(dian)物(wu)(wu)(wu)顯著增(zeng)(zeng)(zeng)多(duo).使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)方(fang)法：1. 本制(zhi)(zhi)品(pin)需(xu)(xu)要(yao)現(xian)采(cai)集(ji)，并經(jing)過(guo)(guo)(guo)(guo)(guo)無(wu)(wu)(wu)菌檸檬酸鈉抗(kang)(kang)凝(ning)處(chu)(chu)(chu)理(li)(li)。如(ru)需(xu)(xu)不(bu)同抗(kang)(kang)凝(ning)劑(ji)，請(qing)(qing)提(ti)(ti)前(qian)咨詢我(wo)(wo)司(si)(si)。2. 本制(zhi)(zhi)品(pin)整(zheng)體呈現(xian)紅(hong)色至(zhi)(zhi)暗(an)紅(hong)色，長(chang)(chang)(chang)時(shi)間靜(jing)置，會(hui)有細(xi)(xi)胞(bao)(bao)(bao)沉(chen)(chen)降在(zai)(zai)底部(bu)。3. 收到本制(zhi)(zhi)品(pin)后(hou)(hou)(hou)(hou)，請(qing)(qing)放(fang)置于(yu)(yu)(yu)4℃冰(bing)(bing)箱(xiang)(xiang)保(bao)(bao)存(cun)(cun)(cun)(cun)，嚴(yan)禁凍(dong)(dong)(dong)(dong)(dong)存(cun)(cun)(cun)(cun)。3. 本制(zhi)(zhi)品(pin)為(wei)現(xian)采(cai)現(xian)用(yong)(yong)(yong)(yong)(yong)，保(bao)(bao)質(zhi)期(qi)(qi)(qi)30天(tian)，收到后(hou)(hou)(hou)(hou)請(qing)(qing)及(ji)(ji)時(shi)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)，建議(yi)2周內(nei)(nei)(nei)用(yong)(yong)(yong)(yong)(yong)完。4. 本制(zhi)(zhi)品(pin)易(yi)產(chan)(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)溶(rong)血(xue)(xue)(xue)(xue)(xue)(xue)現(xian)象，每天(tian)需(xu)(xu)輕(qing)輕(qing)將紅(hong)細(xi)(xi)胞(bao)(bao)(bao)全部(bu)搖(yao)起(qi)(qi)。5. 收到本制(zhi)(zhi)品(pin)后(hou)(hou)(hou)(hou)如(ru)有性(xing)(xing)質(zhi)不(bu)符(fu)，請(qing)(qing)于(yu)(yu)(yu)3日內(nei)(nei)(nei)與(yu)我(wo)(wo)司(si)(si)聯(lian)(lian)系(xi)，以便于(yu)(yu)(yu)我(wo)(wo)司(si)(si)今早給(gei)出(chu)判定(ding)答復。3日后(hou)(hou)(hou)(hou)與(yu)我(wo)(wo)司(si)(si)聯(lian)(lian)系(xi)，我(wo)(wo)司(si)(si)有權判定(ding)非(fei)產(chan)(chan)品(pin)質(zhi)量(liang)問題(ti)抗(kang)(kang)凝(ning)兔(tu)(tu)血(xue)(xue)(xue)(xue)(xue)(xue)(無(wu)(wu)(wu)菌 pet瓶裝(zhuang)(zhuang)(zhuang))血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen) 血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)是(shi)由(you)血(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)去(qu)除(chu)(chu)(chu)纖維(wei)(wei)蛋白(bai)(bai)(bai)(bai)原(yuan)而(er)形(xing)成(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)種很復雜的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)物(wu)(wu)(wu),其組(zu)(zu)成(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)份(fen)雖(sui)大(da)(da)部(bu)分(fen)(fen)(fen)(fen)(fen)已知,但還有一(yi)部(bu)分(fen)(fen)(fen)(fen)(fen)尚不(bu)清(qing)(qing)(qing)(qing)楚,且血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)組(zu)(zu)成(cheng)(cheng)(cheng)(cheng)及(ji)(ji)含(han)(han)量(liang)常(chang)(chang)隨供血(xue)(xue)(xue)(xue)(xue)(xue)動(dong)物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)別(bie)、年(nian)齡、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)(li)條件和(he)營(ying)養(yang)(yang)條件不(bu)同而(er)異。血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)是(shi)血(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)中(zhong)(zhong)不(bu)含(han)(han)纖維(wei)(wei)蛋白(bai)(bai)(bai)(bai)原(yuan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠狀液(ye)體,具(ju)有維(wei)(wei)持血(xue)(xue)(xue)(xue)(xue)(xue)液(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正(zheng)常(chang)(chang)粘度(du)、酸堿度(du)、滲(shen)透壓等(deng)(deng)(deng)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。它主要(yao)由(you)水和(he)各種化(hua)(hua)(hua)學成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)組(zu)(zu)成(cheng)(cheng)(cheng)(cheng),這(zhe)些(xie)化(hua)(hua)(hua)學成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)包括白(bai)(bai)(bai)(bai)蛋白(bai)(bai)(bai)(bai)、α1、α2、β、γ-球蛋白(bai)(bai)(bai)(bai),甘油三酯,總膽(dan)固醇,谷丙(bing)轉氨酶等(deng)(deng)(deng)。血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)中(zhong)(zhong)含(han)(han)有各種血(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)蛋白(bai)(bai)(bai)(bai)、多(duo)肽、脂(zhi)肪、碳水化(hua)(hua)(hua)合(he)物(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)子(zi)、激素、無(wu)(wu)(wu)機物(wu)(wu)(wu)等(deng)(deng)(deng),這(zhe)些(xie)物(wu)(wu)(wu)質(zhi)對生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)或活性(xing)(xing)是(shi)達(da)到生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)(li)平衡的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。實(shi)(shi)(shi)驗(yan)(yan)室(shi)(shi)操(cao)作(zuo)(zuo)(zuo)(zuo)注意(yi)(yi)事(shi)(shi)項(xiang)：1.實(shi)(shi)(shi)驗(yan)(yan)時(shi)根據試(shi)(shi)驗(yan)(yan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情(qing)況(kuang)和(he)性(xing)(xing)質(zhi)進(jin)(jin)行必(bi)(bi)(bi)要(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)防護(hu)。根據試(shi)(shi)驗(yan)(yan)可(ke)(ke)(ke)能發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)危險(xian)事(shi)(shi)故(gu)佩戴(dai)必(bi)(bi)(bi)要(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)防護(hu)工具(ju)，例如(ru)穿好試(shi)(shi)驗(yan)(yan)服，戴(dai)橡膠手套，防護(hu)面(mian)具(ju)，防毒面(mian)具(ju)等(deng)(deng)(deng)。實(shi)(shi)(shi)驗(yan)(yan)前(qian)，要(yao)注意(yi)(yi)清(qing)(qing)(qing)(qing)理(li)(li)試(shi)(shi)驗(yan)(yan)場(chang)周圍(wei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)安全隱(yin)患(huan)。檢(jian)(jian)查(cha)試(shi)(shi)驗(yan)(yan)裝(zhuang)(zhuang)(zhuang)置、藥品(pin)和(he)相關物(wu)(wu)(wu)品(pin)是(shi)否(fou)(fou)有不(bu)符(fu)合(he)要(yao)求的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情(qing)況(kuang)等(deng)(deng)(deng)。2.遵循化(hua)(hua)(hua)學藥品(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)質(zhi)和(he)化(hua)(hua)(hua)學反(fan)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)規(gui)律(lv)，不(bu)盲目(mu)蠻干和(he)主觀臆測(ce)化(hua)(hua)(hua)學反(fan)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)。應(ying)(ying)(ying)根據化(hua)(hua)(hua)學反(fan)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)質(zhi)和(he)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)選擇匹配(pei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)反(fan)應(ying)(ying)(ying)裝(zhuang)(zhuang)(zhuang)置，不(bu)可(ke)(ke)(ke)圖省事(shi)(shi)省去(qu)必(bi)(bi)(bi)要(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)安全措施(shi)。3.經(jing)常(chang)(chang)估計(ji)到實(shi)(shi)(shi)驗(yan)(yan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)危險(xian)性(xing)(xing)實(shi)(shi)(shi)驗(yan)(yan)事(shi)(shi)故(gu)雖(sui)不(bu)可(ke)(ke)(ke)預(yu)測(ce)，但其危險(xian)性(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大(da)(da)小是(shi)可(ke)(ke)(ke)以估計(ji)到的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。即(ji)使(shi)(shi)對不(bu)大(da)(da)了解(jie)(jie)(jie)(jie)(jie)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)驗(yan)(yan)，也(ye)必(bi)(bi)(bi)須推(tui)測(ce)其危險(xian)程(cheng)(cheng)度(du)而(er)制(zhi)(zhi)訂相應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)預(yu)防措施(shi)。象下面(mian)這(zhe)類實(shi)(shi)(shi)驗(yan)(yan)，必(bi)(bi)(bi)須十分(fen)(fen)(fen)(fen)(fen)注意(yi)(yi)。 ①不(bu)了解(jie)(jie)(jie)(jie)(jie)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)反(fan)應(ying)(ying)(ying)及(ji)(ji)操(cao)作(zuo)(zuo)(zuo)(zuo); ②存(cun)(cun)(cun)(cun)在(zai)(zai)多(duo)種危險(xian)性(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)驗(yan)(yan)(如(ru)發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)火災、毒氣(qi)(qi)(qi)等(deng)(deng)(deng)); ③在(zai)(zai)嚴(yan)酷(ku)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)反(fan)應(ying)(ying)(ying)條件(如(ru)高溫(wen)(wen)(wen)(wen)、高壓等(deng)(deng)(deng))下進(jin)(jin)行的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)(shi)驗(yan)(yan)。4.充(chong)分(fen)(fen)(fen)(fen)(fen)作(zuo)(zuo)(zuo)(zuo)好發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)事(shi)(shi)故(gu)時(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)預(yu)防措施(shi)并加(jia)以檢(jian)(jian)查(cha)。平時(shi)注意(yi)(yi)熟悉需(xu)(xu)要(yao)關閉(bi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)龍(long)頭、電氣(qi)(qi)(qi)開(kai)關，滅(mie)火器的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)位(wei)置及(ji)(ji)操(cao)作(zuo)(zuo)(zuo)(zuo)方(fang)法，避免(mian)(mian)發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)事(shi)(shi)故(gu)時(shi)才四(si)處(chu)(chu)(chu)尋找應(ying)(ying)(ying)急的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)品(pin)。5.實(shi)(shi)(shi)驗(yan)(yan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)后(hou)(hou)(hou)(hou)處(chu)(chu)(chu)理(li)(li)。實(shi)(shi)(shi)驗(yan)(yan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)后(hou)(hou)(hou)(hou)處(chu)(chu)(chu)理(li)(li)工作(zuo)(zuo)(zuo)(zuo)，亦屬實(shi)(shi)(shi)驗(yan)(yan)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)成(cheng)(cheng)(cheng)(cheng)部(bu)份(fen)。特別(bie)不(bu)可(ke)(ke)(ke)忽略(lve)回(hui)收溶(rong)劑(ji)和(he)廢液(ye)、廢棄物(wu)(wu)(wu)等(deng)(deng)(deng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)處(chu)(chu)(chu)理(li)(li)。保(bao)(bao)質(zhi)期(qi)(qi)(qi)：1.長(chang)(chang)(chang)期(qi)(qi)(qi)保(bao)(bao)存(cun)(cun)(cun)(cun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)必(bi)(bi)(bi)須儲存(cun)(cun)(cun)(cun)于(yu)(yu)(yu)-20℃ - 70℃低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)(bing)箱(xiang)(xiang)中(zhong)(zhong)。4℃冰(bing)(bing)箱(xiang)(xiang)中(zhong)(zhong)保(bao)(bao)存(cun)(cun)(cun)(cun)時(shi)間切勿(wu)超過(guo)(guo)(guo)(guo)(guo)1個(ge)月。由(you)于(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)結(jie)冰(bing)(bing)時(shi)體積會(hui)增(zeng)(zeng)(zeng)加(jia)約10%,因(yin)(yin)(yin)此(ci)(ci)(ci)，血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)在(zai)(zai)凍(dong)(dong)(dong)(dong)(dong)入(ru)(ru)低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)(bing)箱(xiang)(xiang)前(qian)，必(bi)(bi)(bi)須預(yu)留一(yi)定(ding)體積空間，否(fou)(fou)則易(yi)發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)污(wu)染或玻璃(li)瓶凍(dong)(dong)(dong)(dong)(dong)裂。2.一(yi)般(ban)廠(chang)商提(ti)(ti)供的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)為(wei)無(wu)(wu)(wu)菌，無(wu)(wu)(wu)需(xu)(xu)再(zai)(zai)過(guo)(guo)(guo)(guo)(guo)濾(lv)除(chu)(chu)(chu)菌。如(ru)發(fa)(fa)(fa)現(xian)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)有懸浮物(wu)(wu)(wu)，則可(ke)(ke)(ke)將血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)加(jia)入(ru)(ru)培(pei)(pei)養(yang)(yang)液(ye)內(nei)(nei)(nei)一(yi)起(qi)(qi)過(guo)(guo)(guo)(guo)(guo)濾(lv)，切勿(wu)直(zhi)接(jie)過(guo)(guo)(guo)(guo)(guo)濾(lv)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)。3.血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)： 瓶裝(zhuang)(zhuang)(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)需(xu)(xu)采(cai)用(yong)(yong)(yong)(yong)(yong)逐(zhu)步解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)法：-20℃ 至(zhi)(zhi) -70℃ 低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)(bing)箱(xiang)(xiang)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)放(fang)入(ru)(ru)4℃冰(bing)(bing)箱(xiang)(xiang)中(zhong)(zhong)溶(rong)解(jie)(jie)(jie)(jie)(jie)1天(tian)。然(ran)后(hou)(hou)(hou)(hou)移(yi)入(ru)(ru)室(shi)(shi)溫(wen)(wen)(wen)(wen)，待全部(bu)溶(rong)解(jie)(jie)(jie)(jie)(jie)后(hou)(hou)(hou)(hou)再(zai)(zai)分(fen)(fen)(fen)(fen)(fen)裝(zhuang)(zhuang)(zhuang)。在(zai)(zai)溶(rong)解(jie)(jie)(jie)(jie)(jie)過(guo)(guo)(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)需(xu)(xu)不(bu)斷(duan)輕(qing)輕(qing)搖(yao)晃均(jun)勻(yun)（小心勿(wu)造成(cheng)(cheng)(cheng)(cheng)氣(qi)(qi)(qi)泡），使(shi)(shi)溫(wen)(wen)(wen)(wen)度(du)與(yu)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)均(jun)一(yi)，減少沉(chen)(chen)淀(dian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)。切勿(wu)直(zhi)接(jie)將血(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)從-20℃進(jin)(jin)入(ru)(ru)37℃解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)，這(zhe)樣因(yin)(yin)(yin)溫(wen)(wen)(wen)(wen)度(du)改(gai)變太大(da)(da)，容(rong)易(yi)造成(cheng)(cheng)(cheng)(cheng)蛋白(bai)(bai)(bai)(bai)質(zhi)凝(ning)集(ji)而(er)出(chu)現(xian)沉(chen)(chen)淀(dian)
抗凝(ning)(ning)驢(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)（無(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun) 袋裝(zhuang)）規格(ge)(ge)及(ji)單位:100ml/200ml/500ml保(bao)存:-20℃,保(bao)質(zhi)(zhi)(zhi)(zhi)(zhi)期(qi)見瓶身標(biao)簽主(zhu)(zhu)要(yao)(yao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen): 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)由(you)(you)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)去除(chu)(chu)纖(xian)維(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)而形(xing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)種(zhong)(zhong)(zhong)很(hen)復雜的(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)物(wu)(wu)(wu)(wu),其組(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)份雖大(da)(da)部(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)已知,但(dan)還有(you)(you)(you)一(yi)(yi)(yi)(yi)部(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)尚不(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚(chu),且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)及(ji)含(han)(han)量(liang)(liang)(liang)(liang)(liang)常隨(sui)供(gong)(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)(dong)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)別、年(nian)齡(ling)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)(li)條件和(he)(he)營(ying)養(yang)(yang)條件不(bu)(bu)(bu)(bu)同(tong)而異(yi)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)不(bu)(bu)(bu)(bu)含(han)(han)纖(xian)維(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)膠(jiao)狀(zhuang)(zhuang)液(ye)體(ti),具有(you)(you)(you)維(wei)(wei)持(chi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)正常粘度、酸(suan)(suan)堿度、滲(shen)(shen)透(tou)壓(ya)等作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。它主(zhu)(zhu)要(yao)(yao)由(you)(you)水(shui)(shui)和(he)(he)各(ge)(ge)(ge)(ge)種(zhong)(zhong)(zhong)化(hua)(hua)學成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)組(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng),這(zhe)(zhe)(zhe)些(xie)(xie)化(hua)(hua)學成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)包括(kuo)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)、α1、α2、β、γ-球(qiu)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai),甘(gan)油三酯(zhi),總膽(dan)(dan)固醇,谷(gu)丙(bing)轉氨(an)酶等。 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)含(han)(han)有(you)(you)(you)各(ge)(ge)(ge)(ge)種(zhong)(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)、多肽、脂(zhi)(zhi)肪、碳水(shui)(shui)化(hua)(hua)合(he)物(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)子、激(ji)(ji)(ji)(ji)素(su)(su)、無(wu)(wu)(wu)(wu)機物(wu)(wu)(wu)(wu)等,這(zhe)(zhe)(zhe)些(xie)(xie)物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)對生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)或(huo)(huo)活(huo)(huo)性(xing)(xing)是(shi)(shi)(shi)(shi)達(da)到生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)(li)平衡的(de)(de)(de)(de)(de)(de)(de)(de)(de)。抗凝(ning)(ning)驢(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)的(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)要(yao)(yao)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)：1、提(ti)(ti)供(gong)(gong)激(ji)(ji)(ji)(ji)素(su)(su)和(he)(he)各(ge)(ge)(ge)(ge)種(zhong)(zhong)(zhong)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)子。2、對培養(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)起(qi)(qi)(qi)到某(mou)些(xie)(xie)保(bao)護作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。3、提(ti)(ti)供(gong)(gong)結(jie)(jie)合(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai):結(jie)(jie)合(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)(shi)攜帶重要(yao)(yao)地低(di)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)子量(liang)(liang)(liang)(liang)(liang)物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi),如(ru)(ru)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜帶維(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素(su)(su),脂(zhi)(zhi)肪,以(yi)及(ji)激(ji)(ji)(ji)(ji)素(su)(su)等,轉鐵蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜帶鐵;結(jie)(jie)合(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)過(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)起(qi)(qi)(qi)重要(yao)(yao)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。4、特別適用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)免疫學研(yan)究,全(quan)球(qiu)科(ke)研(yan)人員信(xin)賴的(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)(chan)(chan)品(pin)(pin);提(ti)(ti)供(gong)(gong)基(ji)本(ben)營(ying)養(yang)(yang)物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi),激(ji)(ji)(ji)(ji)素(su)(su),各(ge)(ge)(ge)(ge)種(zhong)(zhong)(zhong)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)子,促(cu)接(jie)觸和(he)(he)伸展(zhan)因(yin)子使(shi)(shi)(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)貼壁免受機械損傷(shang),結(jie)(jie)合(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)對培養(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)起(qi)(qi)(qi)到某(mou)些(xie)(xie)保(bao)護作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。5、提(ti)(ti)供(gong)(gong)基(ji)本(ben)營(ying)養(yang)(yang)物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi):氨(an)基(ji)酸(suan)(suan),維(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素(su)(su),無(wu)(wu)(wu)(wu)機物(wu)(wu)(wu)(wu),脂(zhi)(zhi)類(lei)物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi),核酸(suan)(suan)衍生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)等,是(shi)(shi)(shi)(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)必(bi)須(xu)(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)。用(yong)(yong)(yong)(yong)(yong)(yong)法 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)由(you)(you)冰(bing)(bing)(bing)(bing)凍(dong)(dong)(dong)(dong)狀(zhuang)(zhuang)態在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)常溫(wen)(wen)(wen)下(xia)完(wan)(wan)全(quan)融化(hua)(hua)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)液(ye)體(ti),搖(yao)(yao)均(jun)勻(yun)(yun)后(hou)(hou)放(fang)(fang)置 10-30 分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘待(dai)結(jie)(jie)塊(kuai)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)沉(chen)(chen)底(di)后(hou)(hou)即(ji)可(ke)(ke) 使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)。注意(yi)事項(xiang) 有(you)(you)(you)少量(liang)(liang)(liang)(liang)(liang)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)結(jie)(jie)塊(kuai)析(xi)(xi)出(chu)(chu)(chu)不(bu)(bu)(bu)(bu)影響(xiang)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)。未融化(hua)(hua)或(huo)(huo)融化(hua)(hua)未經搖(yao)(yao)勻(yun)(yun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)禁止(zhi)直(zhi)(zhi)(zhi)接(jie)放(fang)(fang)入(ru)高溫(wen)(wen)(wen)水(shui)(shui)浴內加溫(wen)(wen)(wen)。減(jian)少血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)重復凍(dong)(dong)(dong)(dong)融。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)室溫(wen)(wen)(wen)或(huo)(huo) 4 ℃ 冰(bing)(bing)(bing)(bing)箱內放(fang)(fang)置過(guo)(guo)(guo)(guo)久會(hui)(hui)影響(xiang)促(cu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)效(xiao)果。解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong): 將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)(cong)冰(bing)(bing)(bing)(bing)凍(dong)(dong)(dong)(dong)區(qu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)取(qu)出(chu)(chu)(chu)后(hou)(hou)(切(qie)(qie)(qie)勿(wu)直(zhi)(zhi)(zhi)接(jie)將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)(cong) -20 ℃ 進(jin)(jin)入(ru) 37 ℃ 解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong),這(zhe)(zhe)(zhe)樣因(yin)溫(wen)(wen)(wen)度改(gai)變(bian)太大(da)(da),容易造(zao)(zao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)凝(ning)(ning)集(ji)而出(chu)(chu)(chu)現沉(chen)(chen)淀(dian)),先置于(yu)(yu)(yu) 2 ~ 8 ℃ 區(qu)域中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)使(shi)(shi)(shi)之(zhi)(zhi)融化(hua)(hua),然(ran)后(hou)(hou)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)室溫(wen)(wen)(wen)下(xia)使(shi)(shi)(shi)之(zhi)(zhi)全(quan)融。使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)前(qian)(qian)再(zai)預溫(wen)(wen)(wen)到將(jiang)(jiang)(jiang)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)溫(wen)(wen)(wen)度(如(ru)(ru) 37 ℃ ),但(dan)必(bi)須(xu)(xu)(xu)注意(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi),解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)過(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)應(ying)(ying)輕輕地搖(yao)(yao)晃(huang)均(jun)勻(yun)(yun)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)過(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)可(ke)(ke)能(neng)會(hui)(hui)出(chu)(chu)(chu)現少量(liang)(liang)(liang)(liang)(liang)絮(xu)(xu)(xu)狀(zhuang)(zhuang)或(huo)(huo)片(pian)狀(zhuang)(zhuang)析(xi)(xi)出(chu)(chu)(chu)物(wu)(wu)(wu)(wu),主(zhu)(zhu)要(yao)(yao)是(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)的(de)(de)(de)(de)(de)(de)(de)(de)(de)變(bian)性(xing)(xing)或(huo)(huo)是(shi)(shi)(shi)(shi)纖(xian)維(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(形(xing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)的(de)(de)(de)(de)(de)(de)(de)(de)(de)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)之(zhi)(zhi)一(yi)(yi)(yi)(yi))析(xi)(xi)出(chu)(chu)(chu),但(dan)這(zhe)(zhe)(zhe)些(xie)(xie)析(xi)(xi)出(chu)(chu)(chu)物(wu)(wu)(wu)(wu),并不(bu)(bu)(bu)(bu)影響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本(ben)身的(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)。 若一(yi)(yi)(yi)(yi)次(ci)無(wu)(wu)(wu)(wu)法用(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)一(yi)(yi)(yi)(yi)瓶,建(jian)議(yi)無(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)至(zhi)恰(qia)當的(de)(de)(de)(de)(de)(de)(de)(de)(de)滅(mie)(mie)菌(jun)(jun)(jun)(jun)容器內,再(zai)放(fang)(fang)回冷凍(dong)(dong)(dong)(dong)。注意(yi)須(xu)(xu)(xu)知（僅供(gong)(gong)參考(kao)）：1.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)采用(yong)(yong)(yong)(yong)(yong)(yong)逐(zhu)步(bu)解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)法（-20℃→2—8℃→25℃或(huo)(huo)37℃），解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)過(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)必(bi)須(xu)(xu)(xu)隨(sui)時輕搖(yao)(yao)，使(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)受熱(re)溫(wen)(wen)(wen)度均(jun)勻(yun)(yun)。2.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)凝(ning)(ning)絮(xu)(xu)(xu)物(wu)(wu)(wu)(wu)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)后(hou)(hou)會(hui)(hui)出(chu)(chu)(chu)現少量(liang)(liang)(liang)(liang)(liang)片(pian)狀(zhuang)(zhuang)或(huo)(huo)絮(xu)(xu)(xu)狀(zhuang)(zhuang)物(wu)(wu)(wu)(wu)析(xi)(xi)出(chu)(chu)(chu)，這(zhe)(zhe)(zhe)主(zhu)(zhu)要(yao)(yao)是(shi)(shi)(shi)(shi)由(you)(you)于(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)內不(bu)(bu)(bu)(bu)穩定蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)變(bian)性(xing)(xing)、纖(xian)維(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)析(xi)(xi)出(chu)(chu)(chu)形(xing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)，實驗證(zheng)明(ming)(ming)沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)不(bu)(bu)(bu)(bu)會(hui)(hui)影響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本(ben)身質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)。3.熱(re)滅(mie)(mie)活(huo)(huo)本(ben)產(chan)(chan)(chan)品(pin)(pin)除(chu)(chu)注明(ming)(ming)外均(jun)未滅(mie)(mie)活(huo)(huo)。如(ru)(ru)果必(bi)須(xu)(xu)(xu)滅(mie)(mie)活(huo)(huo)，請嚴(yan)格(ge)(ge)按(an)照56℃30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘（水(shui)(shui)浴）處理(li)(li)已解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。4.常溫(wen)(wen)(wen)狀(zhuang)(zhuang)態下(xia)短期(qi)融化(hua)(hua)并不(bu)(bu)(bu)(bu)會(hui)(hui)影響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)。5.產(chan)(chan)(chan)品(pin)(pin)有(you)(you)(you)效(xiao)期(qi)，檢定合(he)格(ge)(ge)之(zhi)(zhi)日(ri)起(qi)(qi)(qi)有(you)(you)(you)效(xiao)期(qi)5年(nian)。 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)凝(ning)(ning)結(jie)(jie)析(xi)(xi)出(chu)(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)淡(dan)黃色(se)通(tong)(tong)明(ming)(ming)液(ye)體(ti)。如(ru)(ru)將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)自血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管內抽出(chu)(chu)(chu)，放(fang)(fang)入(ru)試(shi)管中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，不(bu)(bu)(bu)(bu)加抗凝(ning)(ning)劑(ji)，則(ze)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反響(xiang)被激(ji)(ji)(ji)(ji)活(huo)(huo)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)敏捷凝(ning)(ning)結(jie)(jie)，構成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)膠(jiao)凍(dong)(dong)(dong)(dong)。其周圍所(suo)(suo)析(xi)(xi)出(chu)(chu)(chu)之(zhi)(zhi)淡(dan)黃色(se)通(tong)(tong)明(ming)(ming)液(ye)體(ti)即(ji)為(wei)(wei)(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，也可(ke)(ke)于(yu)(yu)(yu)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后(hou)(hou)經離心(xin)獲(huo)得(de)。在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)過(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，纖(xian)維(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)轉成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)為(wei)(wei)(wei)纖(xian)維(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)塊(kuai)，所(suo)(suo)以(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)無(wu)(wu)(wu)(wu)纖(xian)維(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)，這(zhe)(zhe)(zhe)一(yi)(yi)(yi)(yi)點(dian)是(shi)(shi)(shi)(shi)與(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)zui大(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)差(cha)(cha)異(yi)。而在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反響(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)板(ban)開(kai)釋出(chu)(chu)(chu)很(hen)多物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)，各(ge)(ge)(ge)(ge)也都(dou)(dou)(dou)發(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)了改(gai)變(bian)。這(zhe)(zhe)(zhe)些(xie)(xie)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)都(dou)(dou)(dou)留(liu)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)并持(chi)續發(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)改(gai)變(bian)，如(ru)(ru)原(yuan)(yuan)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)為(wei)(wei)(wei)，并隨(sui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)寄存時刻逐(zhu)步(bu)削(xue)減(jian)以(yi)致不(bu)(bu)(bu)(bu)見。這(zhe)(zhe)(zhe)些(xie)(xie)也都(dou)(dou)(dou)是(shi)(shi)(shi)(shi)與(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)差(cha)(cha)異(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)地方(fang)(fang)。但(dan)很(hen)多未參與(yu)(yu)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反響(xiang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)則(ze)與(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)根(gen)本(ben)一(yi)(yi)(yi)(yi)樣。為(wei)(wei)(wei)防止(zhi)抗凝(ning)(ning)劑(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)攪擾，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)很(hen)多化(hua)(hua)學成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)剖析(xi)(xi)，都(dou)(dou)(dou)以(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)(wei)(wei)樣品(pin)(pin)。抗凝(ning)(ning)驢(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)（無(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun) 袋裝(zhuang)）血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)要(yao)(yao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)使(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)出(chu)(chu)(chu)去纖(xian)維(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)而形(xing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)種(zhong)(zhong)(zhong)和(he)(he)復雜的(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)物(wu)(wu)(wu)(wu),含(han)(han)有(you)(you)(you)各(ge)(ge)(ge)(ge)種(zhong)(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)、多肽、脂(zhi)(zhi)肪、碳水(shui)(shui)化(hua)(hua)合(he)物(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)子、激(ji)(ji)(ji)(ji)素(su)(su)以(yi)及(ji)無(wu)(wu)(wu)(wu)機物(wu)(wu)(wu)(wu)等,具有(you)(you)(you)維(wei)(wei)持(chi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)正常粘度、酸(suan)(suan)堿度、滲(shen)(shen)透(tou)壓(ya)等作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong),且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)及(ji)其含(han)(han)量(liang)(liang)(liang)(liang)(liang)常隨(sui)動(dong)(dong)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)別、年(nian)齡(ling)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)(li)條件和(he)(he)營(ying)養(yang)(yang)條件的(de)(de)(de)(de)(de)(de)(de)(de)(de)不(bu)(bu)(bu)(bu)同(tong)而異(yi)。它主(zhu)(zhu)要(yao)(yao)由(you)(you)水(shui)(shui)和(he)(he)各(ge)(ge)(ge)(ge)種(zhong)(zhong)(zhong)化(hua)(hua)學成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)組(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng),包括(kuo)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)、α1、α2、β、γ-球(qiu)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)、甘(gan)油三酯(zhi)、總膽(dan)(dan)固醇、谷(gu)丙(bing)轉氨(an)酶等。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)保(bao)存應(ying)(ying)該注意(yi)以(yi)下(xia)幾(ji)點(dian)：(1)需要(yao)(yao)長(chang)(chang)(chang)期(qi)保(bao)存的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必(bi)須(xu)(xu)(xu)儲存于(yu)(yu)(yu)-20℃-70℃低(di)溫(wen)(wen)(wen)冰(bing)(bing)(bing)(bing)箱中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong).4℃冰(bing)(bing)(bing)(bing)箱中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)存時間(jian)切(qie)(qie)(qie)勿(wu)超(chao)過(guo)(guo)(guo)(guo)1個月.由(you)(you)于(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結(jie)(jie)冰(bing)(bing)(bing)(bing)時體(ti)積會(hui)(hui)增加約10%,因(yin)此,血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)凍(dong)(dong)(dong)(dong)入(ru)低(di)溫(wen)(wen)(wen)冰(bing)(bing)(bing)(bing)箱前(qian)(qian),必(bi)須(xu)(xu)(xu)預留(liu)一(yi)(yi)(yi)(yi)定體(ti)積空(kong)間(jian),否(fou)則(ze)易發(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)污(wu)(wu)染或(huo)(huo)玻(bo)璃瓶凍(dong)(dong)(dong)(dong)裂.(2)熱(re)滅(mie)(mie)活(huo)(huo)是(shi)(shi)(shi)(shi)指56℃,30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘加熱(re)已*解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).加熱(re)過(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)須(xu)(xu)(xu)規則(ze)搖(yao)(yao)晃(huang)均(jun)勻(yun)(yun).此熱(re)處理(li)(li)的(de)(de)(de)(de)(de)(de)(de)(de)(de)目的(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)使(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)補體(ti)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(complement)滅(mie)(mie)活(huo)(huo).除(chu)(chu)非必(bi)須(xu)(xu)(xu),一(yi)(yi)(yi)(yi)般(ban)不(bu)(bu)(bu)(bu)建(jian)議(yi)作(zuo)(zuo)(zuo)此熱(re)處理(li)(li),因(yin)為(wei)(wei)(wei)熱(re)處理(li)(li)會(hui)(hui)造(zao)(zao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)顯著增多,而且還會(hui)(hui)影響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang).補體(ti)參與(yu)(yu)反應(ying)(ying)有(you)(you)(you):細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)毒作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong),平滑(hua)肌細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)收縮(suo),肥大(da)(da)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)和(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)板(ban)釋放(fang)(fang)組(zu)(zu)胺,增強(qiang)吞噬(shi)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong),促(cu)進(jin)(jin)淋巴細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)和(he)(he)巨噬(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)發(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)學趨化(hua)(hua)和(he)(he)活(huo)(huo)化(hua)(hua).(3)瓶裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)需采用(yong)(yong)(yong)(yong)(yong)(yong)逐(zhu)步(bu)解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)法:-20℃至(zhi)-70℃低(di)溫(wen)(wen)(wen)冰(bing)(bing)(bing)(bing)箱中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)放(fang)(fang)入(ru)4℃冰(bing)(bing)(bing)(bing)箱中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)溶(rong)解(jie)(jie)(jie)1天.然(ran)后(hou)(hou)移(yi)入(ru)室溫(wen)(wen)(wen),待(dai)全(quan)部(bu)(bu)溶(rong)解(jie)(jie)(jie)后(hou)(hou)再(zai)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang).在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)溶(rong)解(jie)(jie)(jie)過(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)需不(bu)(bu)(bu)(bu)斷輕輕搖(yao)(yao)晃(huang)均(jun)勻(yun)(yun)(小(xiao)(xiao)心(xin)勿(wu)造(zao)(zao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)氣泡),使(shi)(shi)(shi)溫(wen)(wen)(wen)度與(yu)(yu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)均(jun)一(yi)(yi)(yi)(yi),減(jian)少沉(chen)(chen)淀(dian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng).切(qie)(qie)(qie)勿(wu)直(zhi)(zhi)(zhi)接(jie)將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)(cong)-20℃進(jin)(jin)入(ru)37℃解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong),這(zhe)(zhe)(zhe)樣因(yin)溫(wen)(wen)(wen)度改(gai)變(bian)太大(da)(da),容易造(zao)(zao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)凝(ning)(ning)集(ji)而出(chu)(chu)(chu)現沉(chen)(chen)淀(dian).(4)一(yi)(yi)(yi)(yi)般(ban)廠商提(ti)(ti)供(gong)(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)(wei)(wei)無(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun),無(wu)(wu)(wu)(wu)需再(zai)過(guo)(guo)(guo)(guo)濾(lv)除(chu)(chu)菌(jun)(jun)(jun)(jun).如(ru)(ru)發(fa)現血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)有(you)(you)(you)懸(xuan)浮物(wu)(wu)(wu)(wu),則(ze)可(ke)(ke)將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)加入(ru)培養(yang)(yang)液(ye)內一(yi)(yi)(yi)(yi)起(qi)(qi)(qi)過(guo)(guo)(guo)(guo)濾(lv),切(qie)(qie)(qie)勿(wu)直(zhi)(zhi)(zhi)接(jie)過(guo)(guo)(guo)(guo)濾(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).(5)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)絮(xu)(xu)(xu)狀(zhuang)(zhuang)物(wu)(wu)(wu)(wu):主(zhu)(zhu)要(yao)(yao)是(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)變(bian)性(xing)(xing)及(ji)解(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)后(hou)(hou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)纖(xian)維(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)造(zao)(zao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng),這(zhe)(zhe)(zhe)些(xie)(xie)絮(xu)(xu)(xu)狀(zhuang)(zhuang)物(wu)(wu)(wu)(wu)不(bu)(bu)(bu)(bu)會(hui)(hui)影響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本(ben)身的(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang).可(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)離心(xin)3000rpm,5分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘去除(chu)(chu),也可(ke)(ke)不(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)處理(li)(li).顯微鏡下(xia)小(xiao)(xiao)黑點(dian):經過(guo)(guo)(guo)(guo)熱(re)處理(li)(li)過(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)形(xing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)會(hui)(hui)顯著增多.有(you)(you)(you)些(xie)(xie)沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)顯微鏡下(xia)觀察(cha)象(xiang)小(xiao)(xiao)黑點(dian),常誤認(ren)為(wei)(wei)(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)受污(wu)(wu)染.一(yi)(yi)(yi)(yi)般(ban)情況(kuang)下(xia),此小(xiao)(xiao)黑點(dian)不(bu)(bu)(bu)(bu)會(hui)(hui)影響(xiang)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang),但(dan)如(ru)(ru)果懷疑血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang),則(ze)應(ying)(ying)立(li)即(ji)停(ting)止(zhi)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong),更換另一(yi)(yi)(yi)(yi)批(pi)號的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).(6)切(qie)(qie)(qie)勿(wu)將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)37℃放(fang)(fang)置太久,否(fou)則(ze)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)會(hui)(hui)變(bian)得(de)渾濁,同(tong)時血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)(you)效(xiao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)會(hui)(hui)破會(hui)(hui)而影響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang).公司產(chan)(chan)(chan)品(pin)(pin)僅用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)科(ke)研(yan)對于(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)研(yan)究,仍然(ran)存在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)一(yi)(yi)(yi)(yi)些(xie)(xie)未解(jie)(jie)(jie)之(zhi)(zhi)謎:1.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)復雜,可(ke)(ke)能(neng)有(you)(you)(you)幾(ji)百種(zhong)(zhong)(zhong)之(zhi)(zhi)多,對其準確的(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)、含(han)(han)量(liang)(liang)(liang)(liang)(liang)及(ji)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)尚且不(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),尤其是(shi)(shi)(shi)(shi)一(yi)(yi)(yi)(yi)些(xie)(xie)多肽生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)類(lei)因(yin)子、激(ji)(ji)(ji)(ji)素(su)(su)和(he)(he)脂(zhi)(zhi)類(lei)等。2.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都(dou)(dou)(dou)是(shi)(shi)(shi)(shi)批(pi)量(liang)(liang)(liang)(liang)(liang) ,各(ge)(ge)(ge)(ge)批(pi)量(liang)(liang)(liang)(liang)(liang)之(zhi)(zhi)間(jian)差(cha)(cha)異(yi)很(hen)大(da)(da),且保(bao)存期(qi)一(yi)(yi)(yi)(yi)般(ban)不(bu)(bu)(bu)(bu)超(chao)過(guo)(guo)(guo)(guo)一(yi)(yi)(yi)(yi)年(nian),因(yin)此要(yao)(yao)保(bao)證(zheng)每批(pi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)相似(si)性(xing)(xing)幾(ji)乎不(bu)(bu)(bu)(bu)太現實;3.不(bu)(bu)(bu)(bu)能(neng)排除(chu)(chu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)含(han)(han)有(you)(you)(you)易變(bian)物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi),這(zhe)(zhe)(zhe)被認(ren)為(wei)(wei)(wei)是(shi)(shi)(shi)(shi)“瓶中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)”的(de)(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)(yuan)因(yin)之(zhi)(zhi)一(yi)(yi)(yi)(yi)。 質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)標(biao)準血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)高低(di)取(qu)決于(yu)(yu)(yu)兩方(fang)(fang)面因(yin)素(su)(su)：一(yi)(yi)(yi)(yi)是(shi)(shi)(shi)(shi)取(qu)材對象(xiang)，二(er)是(shi)(shi)(shi)(shi)取(qu)材過(guo)(guo)(guo)(guo)程(cheng)。用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)取(qu)材的(de)(de)(de)(de)(de)(de)(de)(de)(de)動(dong)(dong)物(wu)(wu)(wu)(wu)應(ying)(ying)健康無(wu)(wu)(wu)(wu)病(bing)(bing)并且在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)指定的(de)(de)(de)(de)(de)(de)(de)(de)(de)出(chu)(chu)(chu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)天數(shu)之(zhi)(zhi)內，取(qu)材過(guo)(guo)(guo)(guo)程(cheng)應(ying)(ying)嚴(yan)格(ge)(ge)按(an)照操作(zuo)(zuo)(zuo)規程(cheng)執行，制備出(chu)(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)要(yao)(yao)經過(guo)(guo)(guo)(guo)嚴(yan)格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)鑒定。1． 牛(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必(bi)須(xu)(xu)(xu)來(lai)自有(you)(you)(you)文(wen)件證(zheng)明(ming)(ming)無(wu)(wu)(wu)(wu)牛(niu)海綿狀(zhuang)(zhuang)腦(nao)病(bing)(bing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)牛(niu)群或(huo)(huo)國家(jia)。并應(ying)(ying)具備適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)監測(ce)系統。2． 有(you)(you)(you)些(xie)(xie)國家(jia)還要(yao)(yao)求牛(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)來(lai)自未用(yong)(yong)(yong)(yong)(yong)(yong)過(guo)(guo)(guo)(guo)反芻動(dong)(dong)物(wu)(wu)(wu)(wu)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)飼料的(de)(de)(de)(de)(de)(de)(de)(de)(de)牛(niu)群。3． 證(zheng)明(ming)(ming)所(suo)(suo)用(yong)(yong)(yong)(yong)(yong)(yong)牛(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)不(bu)(bu)(bu)(bu)含(han)(han)對所(suo)(suo)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)疫苗病(bing)(bing)毒的(de)(de)(de)(de)(de)(de)(de)(de)(de)抑制物(wu)(wu)(wu)(wu)。4． 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)要(yao)(yao)通(tong)(tong)過(guo)(guo)(guo)(guo)濾(lv)膜過(guo)(guo)(guo)(guo)濾(lv)除(chu)(chu)菌(jun)(jun)(jun)(jun)，保(bao)證(zheng)無(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)。5． 無(wu)(wu)(wu)(wu)細(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)、霉菌(jun)(jun)(jun)(jun)、支(zhi)原(yuan)(yuan)體(ti)和(he)(he)病(bing)(bing)毒的(de)(de)(de)(de)(de)(de)(de)(de)(de)污(wu)(wu)染，有(you)(you)(you)些(xie)(xie)國家(jia)要(yao)(yao)求無(wu)(wu)(wu)(wu)細(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)噬(shi)菌(jun)(jun)(jun)(jun)體(ti)污(wu)(wu)染。6． 對細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)有(you)(you)(you)良(liang)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)支(zhi)持(chi)繁殖作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。
抗(kang)(kang)(kang)凝(ning)(ning)(ning)(ning)驢血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)（無(wu)(wu)菌(jun) pet瓶(ping)(ping)裝(zhuang)）規格(ge)(ge)及(ji)單位:100ml/200ml/500ml 保(bao)存(cun):-20℃,保(bao)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)期(qi)見(jian)瓶(ping)(ping)身(shen)標(biao)簽(qian)主(zhu)(zhu)(zhu)(zhu)要(yao)(yao)(yao)(yao)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen): 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)(shi)由(you)(you)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)去除(chu)纖(xian)維(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)而形成(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)種(zhong)(zhong)(zhong)(zhong)很復雜(za)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu),其(qi)組(zu)成(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)份雖大(da)部(bu)分(fen)(fen)(fen)(fen)已(yi)知,但還有(you)(you)(you)(you)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)部(bu)分(fen)(fen)(fen)(fen)尚(shang)不(bu)(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚,且(qie)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組(zu)成(cheng)(cheng)(cheng)(cheng)及(ji)含(han)(han)量(liang)(liang)(liang)(liang)常(chang)隨(sui)供血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)別、年齡、生(sheng)(sheng)(sheng)(sheng)理(li)(li)(li)條(tiao)(tiao)件和(he)(he)(he)(he)營(ying)養(yang)(yang)(yang)條(tiao)(tiao)件不(bu)(bu)(bu)(bu)(bu)同(tong)而異(yi)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)中(zhong)(zhong)(zhong)(zhong)(zhong)不(bu)(bu)(bu)(bu)(bu)含(han)(han)纖(xian)維(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠狀(zhuang)液體(ti)(ti)(ti),具有(you)(you)(you)(you)維(wei)(wei)(wei)持血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正常(chang)粘(zhan)度、酸(suan)堿度、滲透壓等(deng)(deng)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。它(ta)主(zhu)(zhu)(zhu)(zhu)要(yao)(yao)(yao)(yao)由(you)(you)水和(he)(he)(he)(he)各種(zhong)(zhong)(zhong)(zhong)化(hua)(hua)(hua)學(xue)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)組(zu)成(cheng)(cheng)(cheng)(cheng),這(zhe)(zhe)些化(hua)(hua)(hua)學(xue)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)包括白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、α1、α2、β、γ-球蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai),甘油(you)三(san)酯,總(zong)膽固(gu)醇,谷(gu)丙轉(zhuan)氨酶等(deng)(deng)。 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)含(han)(han)有(you)(you)(you)(you)各種(zhong)(zhong)(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、多(duo)(duo)肽、脂(zhi)(zhi)肪、碳水化(hua)(hua)(hua)合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)(yin)子(zi)、激(ji)(ji)素(su)、無(wu)(wu)機(ji)(ji)(ji)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)等(deng)(deng),這(zhe)(zhe)些物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)對生(sheng)(sheng)(sheng)(sheng)長(chang)或(huo)活(huo)性(xing)是(shi)(shi)(shi)(shi)(shi)(shi)達(da)到(dao)(dao)生(sheng)(sheng)(sheng)(sheng)理(li)(li)(li)平衡(heng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。抗(kang)(kang)(kang)凝(ning)(ning)(ning)(ning)驢血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)(zhu)(zhu)要(yao)(yao)(yao)(yao)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)：1、提(ti)(ti)供激(ji)(ji)素(su)和(he)(he)(he)(he)各種(zhong)(zhong)(zhong)(zhong)生(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)(yin)子(zi)。2、對培(pei)(pei)養(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細胞(bao)(bao)(bao)(bao)(bao)起(qi)到(dao)(dao)某些保(bao)護(hu)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。3、提(ti)(ti)供結(jie)(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai):結(jie)(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)(shi)(shi)(shi)攜帶重(zhong)要(yao)(yao)(yao)(yao)地低(di)(di)分(fen)(fen)(fen)(fen)子(zi)量(liang)(liang)(liang)(liang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi),如(ru)(ru)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜帶維(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)素(su),脂(zhi)(zhi)肪,以及(ji)激(ji)(ji)素(su)等(deng)(deng),轉(zhuan)鐵蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜帶鐵;結(jie)(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)在(zai)過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)起(qi)重(zhong)要(yao)(yao)(yao)(yao)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。4、特別適用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)免(mian)疫(yi)學(xue)研(yan)究,全(quan)球科研(yan)人員信賴(lai)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)(chan)品;提(ti)(ti)供基(ji)本(ben)(ben)營(ying)養(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi),激(ji)(ji)素(su),各種(zhong)(zhong)(zhong)(zhong)生(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)(yin)子(zi),促接(jie)觸和(he)(he)(he)(he)伸展(zhan)因(yin)(yin)(yin)(yin)子(zi)使(shi)(shi)(shi)細胞(bao)(bao)(bao)(bao)(bao)貼壁免(mian)受(shou)機(ji)(ji)(ji)械損(sun)傷,結(jie)(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)對培(pei)(pei)養(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細胞(bao)(bao)(bao)(bao)(bao)起(qi)到(dao)(dao)某些保(bao)護(hu)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。5、提(ti)(ti)供基(ji)本(ben)(ben)營(ying)養(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi):氨基(ji)酸(suan),維(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)素(su),無(wu)(wu)機(ji)(ji)(ji)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu),脂(zhi)(zhi)類物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi),核酸(suan)衍(yan)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)等(deng)(deng),是(shi)(shi)(shi)(shi)(shi)(shi)細胞(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長(chang)必(bi)(bi)(bi)(bi)須(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。用(yong)(yong)(yong)(yong)(yong)法(fa) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)由(you)(you)冰(bing)凍(dong)(dong)(dong)(dong)(dong)狀(zhuang)態在(zai)常(chang)溫(wen)(wen)(wen)(wen)下(xia)(xia)完(wan)全(quan)融化(hua)(hua)(hua)成(cheng)(cheng)(cheng)(cheng)液體(ti)(ti)(ti),搖均勻(yun)(yun)(yun)(yun)后放(fang)(fang)(fang)置(zhi) 10-30 分(fen)(fen)(fen)(fen)鐘(zhong)待結(jie)(jie)塊(kuai)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)沉(chen)底后即可(ke) 使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。注意(yi)事項 有(you)(you)(you)(you)少(shao)量(liang)(liang)(liang)(liang)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)結(jie)(jie)塊(kuai)析(xi)出(chu)(chu)(chu)不(bu)(bu)(bu)(bu)(bu)影(ying)(ying)響(xiang)(xiang)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。未融化(hua)(hua)(hua)或(huo)融化(hua)(hua)(hua)未經(jing)搖勻(yun)(yun)(yun)(yun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)禁止(zhi)直(zhi)接(jie)放(fang)(fang)(fang)入高(gao)(gao)溫(wen)(wen)(wen)(wen)水浴(yu)內加(jia)(jia)(jia)(jia)溫(wen)(wen)(wen)(wen)。減(jian)少(shao)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)重(zhong)復凍(dong)(dong)(dong)(dong)(dong)融。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)室溫(wen)(wen)(wen)(wen)或(huo) 4 ℃ 冰(bing)箱(xiang)(xiang)(xiang)內放(fang)(fang)(fang)置(zhi)過(guo)(guo)久(jiu)會(hui)影(ying)(ying)響(xiang)(xiang)促細胞(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長(chang)效(xiao)果。解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong): 將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)冰(bing)凍(dong)(dong)(dong)(dong)(dong)區中(zhong)(zhong)(zhong)(zhong)(zhong)取出(chu)(chu)(chu)后(切勿直(zhi)接(jie)將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong) -20 ℃ 進(jin)入 37 ℃ 解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong),這(zhe)(zhe)樣(yang)因(yin)(yin)(yin)(yin)溫(wen)(wen)(wen)(wen)度改(gai)變(bian)(bian)(bian)太(tai)(tai)大(da),容易造(zao)成(cheng)(cheng)(cheng)(cheng)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)凝(ning)(ning)(ning)(ning)集(ji)而出(chu)(chu)(chu)現(xian)(xian)(xian)沉(chen)淀(dian)(dian)(dian)),先置(zhi)于(yu)(yu) 2 ~ 8 ℃ 區域中(zhong)(zhong)(zhong)(zhong)(zhong)使(shi)(shi)(shi)之(zhi)(zhi)融化(hua)(hua)(hua),然(ran)后在(zai)室溫(wen)(wen)(wen)(wen)下(xia)(xia)使(shi)(shi)(shi)之(zhi)(zhi)全(quan)融。使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)前(qian)再(zai)預溫(wen)(wen)(wen)(wen)到(dao)(dao)將(jiang)(jiang)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)溫(wen)(wen)(wen)(wen)度(如(ru)(ru) 37 ℃ ),但必(bi)(bi)(bi)(bi)須(xu)注意(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi),解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)應(ying)(ying)(ying)(ying)輕(qing)輕(qing)地搖晃(huang)(huang)均勻(yun)(yun)(yun)(yun)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)可(ke)能會(hui)出(chu)(chu)(chu)現(xian)(xian)(xian)少(shao)量(liang)(liang)(liang)(liang)絮(xu)狀(zhuang)或(huo)片狀(zhuang)析(xi)出(chu)(chu)(chu)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu),主(zhu)(zhu)(zhu)(zhu)要(yao)(yao)(yao)(yao)是(shi)(shi)(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)變(bian)(bian)(bian)性(xing)或(huo)是(shi)(shi)(shi)(shi)(shi)(shi)纖(xian)維(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(形成(cheng)(cheng)(cheng)(cheng)凝(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)之(zhi)(zhi)一(yi)(yi)(yi)(yi)(yi)(yi)(yi))析(xi)出(chu)(chu)(chu),但這(zhe)(zhe)些析(xi)出(chu)(chu)(chu)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu),并不(bu)(bu)(bu)(bu)(bu)影(ying)(ying)響(xiang)(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本(ben)(ben)身(shen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)。 若一(yi)(yi)(yi)(yi)(yi)(yi)(yi)次(ci)無(wu)(wu)法(fa)用(yong)(yong)(yong)(yong)(yong)完(wan)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)瓶(ping)(ping),建(jian)議(yi)無(wu)(wu)菌(jun)分(fen)(fen)(fen)(fen)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)至恰當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)滅菌(jun)容器內,再(zai)放(fang)(fang)(fang)回冷凍(dong)(dong)(dong)(dong)(dong)。注意(yi)須(xu)知（僅(jin)供參(can)(can)考）：1.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)采(cai)用(yong)(yong)(yong)(yong)(yong)逐步解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)法(fa)（-20℃→2—8℃→25℃或(huo)37℃），解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)必(bi)(bi)(bi)(bi)須(xu)隨(sui)時輕(qing)搖，使(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)受(shou)熱(re)(re)溫(wen)(wen)(wen)(wen)度均勻(yun)(yun)(yun)(yun)。2.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)凝(ning)(ning)(ning)(ning)絮(xu)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)后會(hui)出(chu)(chu)(chu)現(xian)(xian)(xian)少(shao)量(liang)(liang)(liang)(liang)片狀(zhuang)或(huo)絮(xu)狀(zhuang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)析(xi)出(chu)(chu)(chu)，這(zhe)(zhe)主(zhu)(zhu)(zhu)(zhu)要(yao)(yao)(yao)(yao)是(shi)(shi)(shi)(shi)(shi)(shi)由(you)(you)于(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)內不(bu)(bu)(bu)(bu)(bu)穩定(ding)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)變(bian)(bian)(bian)性(xing)、纖(xian)維(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)析(xi)出(chu)(chu)(chu)形成(cheng)(cheng)(cheng)(cheng)沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)，實驗證(zheng)明(ming)(ming)(ming)沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)不(bu)(bu)(bu)(bu)(bu)會(hui)影(ying)(ying)響(xiang)(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本(ben)(ben)身(shen)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)。3.熱(re)(re)滅活(huo)本(ben)(ben)產(chan)(chan)品除(chu)注明(ming)(ming)(ming)外均未滅活(huo)。如(ru)(ru)果必(bi)(bi)(bi)(bi)須(xu)滅活(huo)，請(qing)嚴(yan)(yan)格(ge)(ge)按照56℃30分(fen)(fen)(fen)(fen)鐘(zhong)（水浴(yu)）處理(li)(li)(li)已(yi)解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。4.常(chang)溫(wen)(wen)(wen)(wen)狀(zhuang)態下(xia)(xia)短期(qi)融化(hua)(hua)(hua)并不(bu)(bu)(bu)(bu)(bu)會(hui)影(ying)(ying)響(xiang)(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)。5.產(chan)(chan)品有(you)(you)(you)(you)效(xiao)期(qi)，檢定(ding)合(he)(he)格(ge)(ge)之(zhi)(zhi)日起(qi)有(you)(you)(you)(you)效(xiao)期(qi)5年。 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液凝(ning)(ning)(ning)(ning)結(jie)(jie)析(xi)出(chu)(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)淡(dan)黃(huang)色通明(ming)(ming)(ming)液體(ti)(ti)(ti)。如(ru)(ru)將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液自血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管(guan)內抽(chou)出(chu)(chu)(chu)，放(fang)(fang)(fang)入試(shi)管(guan)中(zhong)(zhong)(zhong)(zhong)(zhong)，不(bu)(bu)(bu)(bu)(bu)加(jia)(jia)(jia)(jia)抗(kang)(kang)(kang)凝(ning)(ning)(ning)(ning)劑，則凝(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)響(xiang)(xiang)被(bei)激(ji)(ji)活(huo)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液敏捷凝(ning)(ning)(ning)(ning)結(jie)(jie)，構成(cheng)(cheng)(cheng)(cheng)膠凍(dong)(dong)(dong)(dong)(dong)。其(qi)周圍所(suo)析(xi)出(chu)(chu)(chu)之(zhi)(zhi)淡(dan)黃(huang)色通明(ming)(ming)(ming)液體(ti)(ti)(ti)即為(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，也(ye)可(ke)于(yu)(yu)凝(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后經(jing)離(li)心(xin)(xin)(xin)獲得(de)。在(zai)凝(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)，纖(xian)維(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)轉(zhuan)成(cheng)(cheng)(cheng)(cheng)為(wei)纖(xian)維(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)塊(kuai)，所(suo)以血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)無(wu)(wu)纖(xian)維(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)，這(zhe)(zhe)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)點(dian)是(shi)(shi)(shi)(shi)(shi)(shi)與(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)zui大(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)差異(yi)。而在(zai)凝(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)響(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小板開釋出(chu)(chu)(chu)很多(duo)(duo)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，各也(ye)都(dou)(dou)發(fa)生(sheng)(sheng)(sheng)(sheng)了(le)改(gai)變(bian)(bian)(bian)。這(zhe)(zhe)些成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)都(dou)(dou)留在(zai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)并持續發(fa)生(sheng)(sheng)(sheng)(sheng)改(gai)變(bian)(bian)(bian)，如(ru)(ru)原(yuan)成(cheng)(cheng)(cheng)(cheng)為(wei)，并隨(sui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)寄(ji)存(cun)時刻逐步削減(jian)以致不(bu)(bu)(bu)(bu)(bu)見(jian)。這(zhe)(zhe)些也(ye)都(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)與(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)差異(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)地方。但很多(duo)(duo)未參(can)(can)與(yu)(yu)(yu)凝(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)響(xiang)(xiang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)則與(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)根本(ben)(ben)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)樣(yang)。為(wei)防(fang)止(zhi)抗(kang)(kang)(kang)凝(ning)(ning)(ning)(ning)劑的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)攪擾(rao)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液中(zhong)(zhong)(zhong)(zhong)(zhong)很多(duo)(duo)化(hua)(hua)(hua)學(xue)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)剖析(xi)，都(dou)(dou)以血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)樣(yang)品。抗(kang)(kang)(kang)凝(ning)(ning)(ning)(ning)驢血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)（無(wu)(wu)菌(jun) pet瓶(ping)(ping)裝(zhuang)）血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)(zhu)(zhu)要(yao)(yao)(yao)(yao)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)使(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)出(chu)(chu)(chu)去纖(xian)維(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)而形成(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)種(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)復雜(za)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu),含(han)(han)有(you)(you)(you)(you)各種(zhong)(zhong)(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、多(duo)(duo)肽、脂(zhi)(zhi)肪、碳水化(hua)(hua)(hua)合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)(yin)子(zi)、激(ji)(ji)素(su)以及(ji)無(wu)(wu)機(ji)(ji)(ji)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)等(deng)(deng),具有(you)(you)(you)(you)維(wei)(wei)(wei)持血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正常(chang)粘(zhan)度、酸(suan)堿度、滲透壓等(deng)(deng)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong),且(qie)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)及(ji)其(qi)含(han)(han)量(liang)(liang)(liang)(liang)常(chang)隨(sui)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)別、年齡、生(sheng)(sheng)(sheng)(sheng)理(li)(li)(li)條(tiao)(tiao)件和(he)(he)(he)(he)營(ying)養(yang)(yang)(yang)條(tiao)(tiao)件的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)不(bu)(bu)(bu)(bu)(bu)同(tong)而異(yi)。它(ta)主(zhu)(zhu)(zhu)(zhu)要(yao)(yao)(yao)(yao)由(you)(you)水和(he)(he)(he)(he)各種(zhong)(zhong)(zhong)(zhong)化(hua)(hua)(hua)學(xue)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)組(zu)成(cheng)(cheng)(cheng)(cheng),包括白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、α1、α2、β、γ-球蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、甘油(you)三(san)酯、總(zong)膽固(gu)醇、谷(gu)丙轉(zhuan)氨酶等(deng)(deng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)保(bao)存(cun)應(ying)(ying)(ying)(ying)該注意(yi)以下(xia)(xia)幾點(dian)：(1)需(xu)要(yao)(yao)(yao)(yao)長(chang)期(qi)保(bao)存(cun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必(bi)(bi)(bi)(bi)須(xu)儲存(cun)于(yu)(yu)-20℃-70℃低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)箱(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong).4℃冰(bing)箱(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)存(cun)時間(jian)切勿超過(guo)(guo)1個月.由(you)(you)于(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結(jie)(jie)冰(bing)時體(ti)(ti)(ti)積會(hui)增(zeng)加(jia)(jia)(jia)(jia)約(yue)10%,因(yin)(yin)(yin)(yin)此(ci)(ci)(ci),血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)凍(dong)(dong)(dong)(dong)(dong)入低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)箱(xiang)(xiang)(xiang)前(qian),必(bi)(bi)(bi)(bi)須(xu)預留一(yi)(yi)(yi)(yi)(yi)(yi)(yi)定(ding)體(ti)(ti)(ti)積空間(jian),否(fou)則易發(fa)生(sheng)(sheng)(sheng)(sheng)污染或(huo)玻璃瓶(ping)(ping)凍(dong)(dong)(dong)(dong)(dong)裂.(2)熱(re)(re)滅活(huo)是(shi)(shi)(shi)(shi)(shi)(shi)指56℃,30分(fen)(fen)(fen)(fen)鐘(zhong)加(jia)(jia)(jia)(jia)熱(re)(re)已(yi)*解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).加(jia)(jia)(jia)(jia)熱(re)(re)過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)須(xu)規則搖晃(huang)(huang)均勻(yun)(yun)(yun)(yun).此(ci)(ci)(ci)熱(re)(re)處理(li)(li)(li)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)目的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)使(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補(bu)體(ti)(ti)(ti)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(complement)滅活(huo).除(chu)非(fei)必(bi)(bi)(bi)(bi)須(xu),一(yi)(yi)(yi)(yi)(yi)(yi)(yi)般(ban)不(bu)(bu)(bu)(bu)(bu)建(jian)議(yi)作(zuo)(zuo)(zuo)此(ci)(ci)(ci)熱(re)(re)處理(li)(li)(li),因(yin)(yin)(yin)(yin)為(wei)熱(re)(re)處理(li)(li)(li)會(hui)造(zao)成(cheng)(cheng)(cheng)(cheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)顯著增(zeng)多(duo)(duo),而且(qie)還會(hui)影(ying)(ying)響(xiang)(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang).補(bu)體(ti)(ti)(ti)參(can)(can)與(yu)(yu)(yu)反(fan)應(ying)(ying)(ying)(ying)有(you)(you)(you)(you):細胞(bao)(bao)(bao)(bao)(bao)毒作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong),平滑肌細胞(bao)(bao)(bao)(bao)(bao)收縮,肥大(da)細胞(bao)(bao)(bao)(bao)(bao)和(he)(he)(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小板釋放(fang)(fang)(fang)組(zu)胺(an),增(zeng)強吞噬(shi)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong),促進(jin)淋(lin)巴細胞(bao)(bao)(bao)(bao)(bao)和(he)(he)(he)(he)巨噬(shi)細胞(bao)(bao)(bao)(bao)(bao)發(fa)生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)學(xue)趨化(hua)(hua)(hua)和(he)(he)(he)(he)活(huo)化(hua)(hua)(hua).(3)瓶(ping)(ping)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)需(xu)采(cai)用(yong)(yong)(yong)(yong)(yong)逐步解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)法(fa):-20℃至-70℃低(di)(di)溫(wen)(wen)(wen)(wen)冰(bing)箱(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)放(fang)(fang)(fang)入4℃冰(bing)箱(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)溶解(jie)(jie)(jie)(jie)1天.然(ran)后移(yi)入室溫(wen)(wen)(wen)(wen),待全(quan)部(bu)溶解(jie)(jie)(jie)(jie)后再(zai)分(fen)(fen)(fen)(fen)裝(zhuang).在(zai)溶解(jie)(jie)(jie)(jie)過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)需(xu)不(bu)(bu)(bu)(bu)(bu)斷輕(qing)輕(qing)搖晃(huang)(huang)均勻(yun)(yun)(yun)(yun)(小心(xin)(xin)(xin)勿造(zao)成(cheng)(cheng)(cheng)(cheng)氣(qi)泡),使(shi)(shi)(shi)溫(wen)(wen)(wen)(wen)度與(yu)(yu)(yu)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)均一(yi)(yi)(yi)(yi)(yi)(yi)(yi),減(jian)少(shao)沉(chen)淀(dian)(dian)(dian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)生(sheng)(sheng)(sheng)(sheng).切勿直(zhi)接(jie)將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)-20℃進(jin)入37℃解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong),這(zhe)(zhe)樣(yang)因(yin)(yin)(yin)(yin)溫(wen)(wen)(wen)(wen)度改(gai)變(bian)(bian)(bian)太(tai)(tai)大(da),容易造(zao)成(cheng)(cheng)(cheng)(cheng)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)凝(ning)(ning)(ning)(ning)集(ji)而出(chu)(chu)(chu)現(xian)(xian)(xian)沉(chen)淀(dian)(dian)(dian).(4)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)般(ban)廠商提(ti)(ti)供的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)無(wu)(wu)菌(jun),無(wu)(wu)需(xu)再(zai)過(guo)(guo)濾(lv)除(chu)菌(jun).如(ru)(ru)發(fa)現(xian)(xian)(xian)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)有(you)(you)(you)(you)懸浮物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu),則可(ke)將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)加(jia)(jia)(jia)(jia)入培(pei)(pei)養(yang)(yang)(yang)液內一(yi)(yi)(yi)(yi)(yi)(yi)(yi)起(qi)過(guo)(guo)濾(lv),切勿直(zhi)接(jie)過(guo)(guo)濾(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).(5)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)絮(xu)狀(zhuang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu):主(zhu)(zhu)(zhu)(zhu)要(yao)(yao)(yao)(yao)是(shi)(shi)(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)變(bian)(bian)(bian)性(xing)及(ji)解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)后血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)纖(xian)維(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)造(zao)成(cheng)(cheng)(cheng)(cheng),這(zhe)(zhe)些絮(xu)狀(zhuang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)不(bu)(bu)(bu)(bu)(bu)會(hui)影(ying)(ying)響(xiang)(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本(ben)(ben)身(shen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang).可(ke)用(yong)(yong)(yong)(yong)(yong)離(li)心(xin)(xin)(xin)3000rpm,5分(fen)(fen)(fen)(fen)鐘(zhong)去除(chu),也(ye)可(ke)不(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)處理(li)(li)(li).顯微鏡下(xia)(xia)小黑點(dian):經(jing)過(guo)(guo)熱(re)(re)處理(li)(li)(li)過(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)形成(cheng)(cheng)(cheng)(cheng)會(hui)顯著增(zeng)多(duo)(duo).有(you)(you)(you)(you)些沉(chen)淀(dian)(dian)(dian)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)在(zai)顯微鏡下(xia)(xia)觀察象(xiang)(xiang)小黑點(dian),常(chang)誤認為(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)受(shou)污染.一(yi)(yi)(yi)(yi)(yi)(yi)(yi)般(ban)情況下(xia)(xia),此(ci)(ci)(ci)小黑點(dian)不(bu)(bu)(bu)(bu)(bu)會(hui)影(ying)(ying)響(xiang)(xiang)細胞(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長(chang),但如(ru)(ru)果懷疑血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang),則應(ying)(ying)(ying)(ying)立即停止(zhi)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong),更換另一(yi)(yi)(yi)(yi)(yi)(yi)(yi)批號的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).(6)切勿將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)37℃放(fang)(fang)(fang)置(zhi)太(tai)(tai)久(jiu),否(fou)則血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)會(hui)變(bian)(bian)(bian)得(de)渾(hun)濁,同(tong)時血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)(you)(you)效(xiao)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)會(hui)破會(hui)而影(ying)(ying)響(xiang)(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang).公司產(chan)(chan)品僅(jin)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)科研(yan)對于(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)研(yan)究,仍然(ran)存(cun)在(zai)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)些未解(jie)(jie)(jie)(jie)之(zhi)(zhi)謎(mi):1.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)復雜(za),可(ke)能有(you)(you)(you)(you)幾百種(zhong)(zhong)(zhong)(zhong)之(zhi)(zhi)多(duo)(duo),對其(qi)準確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)、含(han)(han)量(liang)(liang)(liang)(liang)及(ji)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)尚(shang)且(qie)不(bu)(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),尤其(qi)是(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)些多(duo)(duo)肽生(sheng)(sheng)(sheng)(sheng)長(chang)類因(yin)(yin)(yin)(yin)子(zi)、激(ji)(ji)素(su)和(he)(he)(he)(he)脂(zhi)(zhi)類等(deng)(deng)。2.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)批量(liang)(liang)(liang)(liang) ,各批量(liang)(liang)(liang)(liang)之(zhi)(zhi)間(jian)差異(yi)很大(da),且(qie)保(bao)存(cun)期(qi)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)般(ban)不(bu)(bu)(bu)(bu)(bu)超過(guo)(guo)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)年,因(yin)(yin)(yin)(yin)此(ci)(ci)(ci)要(yao)(yao)(yao)(yao)保(bao)證(zheng)每批血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)相似性(xing)幾乎(hu)不(bu)(bu)(bu)(bu)(bu)太(tai)(tai)現(xian)(xian)(xian)實;3.不(bu)(bu)(bu)(bu)(bu)能排除(chu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)含(han)(han)有(you)(you)(you)(you)易變(bian)(bian)(bian)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi),這(zhe)(zhe)被(bei)認為(wei)是(shi)(shi)(shi)(shi)(shi)(shi)“瓶(ping)(ping)中(zhong)(zhong)(zhong)(zhong)(zhong)”的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)因(yin)(yin)(yin)(yin)之(zhi)(zhi)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)。 質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)標(biao)準血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)高(gao)(gao)低(di)(di)取決于(yu)(yu)兩方面因(yin)(yin)(yin)(yin)素(su)：一(yi)(yi)(yi)(yi)(yi)(yi)(yi)是(shi)(shi)(shi)(shi)(shi)(shi)取材(cai)對象(xiang)(xiang)，二是(shi)(shi)(shi)(shi)(shi)(shi)取材(cai)過(guo)(guo)程。用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)取材(cai)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)應(ying)(ying)(ying)(ying)健(jian)康無(wu)(wu)病(bing)并且(qie)在(zai)指定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)出(chu)(chu)(chu)生(sheng)(sheng)(sheng)(sheng)天數之(zhi)(zhi)內，取材(cai)過(guo)(guo)程應(ying)(ying)(ying)(ying)嚴(yan)(yan)格(ge)(ge)按照操作(zuo)(zuo)(zuo)規程執行，制備出(chu)(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)要(yao)(yao)(yao)(yao)經(jing)過(guo)(guo)嚴(yan)(yan)格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)鑒定(ding)。1． 牛(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必(bi)(bi)(bi)(bi)須(xu)來(lai)自有(you)(you)(you)(you)文件證(zheng)明(ming)(ming)(ming)無(wu)(wu)牛(niu)海綿狀(zhuang)腦病(bing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)牛(niu)群(qun)(qun)或(huo)國家。并應(ying)(ying)(ying)(ying)具備適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)監測系(xi)統。2． 有(you)(you)(you)(you)些國家還要(yao)(yao)(yao)(yao)求牛(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)來(lai)自未用(yong)(yong)(yong)(yong)(yong)過(guo)(guo)反(fan)芻動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)飼料的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)牛(niu)群(qun)(qun)。3． 證(zheng)明(ming)(ming)(ming)所(suo)用(yong)(yong)(yong)(yong)(yong)牛(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)不(bu)(bu)(bu)(bu)(bu)含(han)(han)對所(suo)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)疫(yi)苗病(bing)毒的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)抑(yi)制物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)。4． 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)要(yao)(yao)(yao)(yao)通過(guo)(guo)濾(lv)膜過(guo)(guo)濾(lv)除(chu)菌(jun)，保(bao)證(zheng)無(wu)(wu)菌(jun)。5． 無(wu)(wu)細菌(jun)、霉菌(jun)、支原(yuan)體(ti)(ti)(ti)和(he)(he)(he)(he)病(bing)毒的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)污染，有(you)(you)(you)(you)些國家要(yao)(yao)(yao)(yao)求無(wu)(wu)細菌(jun)噬(shi)菌(jun)體(ti)(ti)(ti)污染。6． 對細胞(bao)(bao)(bao)(bao)(bao)有(you)(you)(you)(you)良好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)支持繁(fan)殖作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。
抗(kang)(kang)(kang)(kang)凝(ning)(ning)(ning)(ning)(ning)馬(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)（無(wu)(wu)菌 袋裝(zhuang)）用(yong)(yong)(yong)(yong)(yong)(yong)(yong)法(fa) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)由冰(bing)凍(dong)(dong)狀(zhuang)(zhuang)(zhuang)(zhuang)態在(zai)常溫(wen)(wen)(wen)(wen)下(xia)(xia)(xia)完(wan)全(quan)(quan)融(rong)(rong)化(hua)(hua)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)液(ye)體(ti)(ti),搖(yao)均(jun)(jun)勻后放(fang)(fang)置(zhi) 10-30 分(fen)(fen)(fen)(fen)(fen)鐘(zhong)待(dai)結塊(kuai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)沉(chen)(chen)(chen)(chen)底后即可 使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。注意事項(xiang) 有(you)(you)(you)(you)少(shao)量(liang)(liang)(liang)(liang)(liang)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)結塊(kuai)析(xi)(xi)出(chu)(chu)(chu)(chu)(chu)不(bu)(bu)(bu)影(ying)(ying)(ying)(ying)響(xiang)(xiang)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。未(wei)(wei)融(rong)(rong)化(hua)(hua)或(huo)(huo)融(rong)(rong)化(hua)(hua)未(wei)(wei)經(jing)搖(yao)勻的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)禁止(zhi)(zhi)(zhi)直(zhi)接放(fang)(fang)入(ru)(ru)高溫(wen)(wen)(wen)(wen)水浴內(nei)加(jia)(jia)(jia)(jia)溫(wen)(wen)(wen)(wen)。減(jian)少(shao)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)重(zhong)復(fu)凍(dong)(dong)融(rong)(rong)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)室(shi)(shi)溫(wen)(wen)(wen)(wen)或(huo)(huo) 4 ℃ 冰(bing)箱(xiang)內(nei)放(fang)(fang)置(zhi)過(guo)(guo)(guo)久會(hui)(hui)(hui)(hui)(hui)影(ying)(ying)(ying)(ying)響(xiang)(xiang)促(cu)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)長(chang)(chang)效果(guo)(guo)。抗(kang)(kang)(kang)(kang)凝(ning)(ning)(ning)(ning)(ning)馬(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(無(wu)(wu)菌 袋裝(zhuang)) 的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)(jie)(jie)(jie)凍(dong)(dong): 將(jiang)(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)冰(bing)凍(dong)(dong)區中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)取(qu)(qu)出(chu)(chu)(chu)(chu)(chu)后(切(qie)(qie)勿(wu)(wu)(wu)直(zhi)接將(jiang)(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong) -20 ℃ 進入(ru)(ru) 37 ℃ 解(jie)(jie)(jie)(jie)凍(dong)(dong),這樣(yang)因(yin)溫(wen)(wen)(wen)(wen)度(du)(du)(du)改(gai)(gai)變(bian)太大,容(rong)易造成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)凝(ning)(ning)(ning)(ning)(ning)集而(er)(er)(er)出(chu)(chu)(chu)(chu)(chu)現(xian)沉(chen)(chen)(chen)(chen)淀),先置(zhi)于(yu)(yu) 2 ~ 8 ℃ 區域中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)使(shi)(shi)(shi)(shi)之融(rong)(rong)化(hua)(hua),然后在(zai)室(shi)(shi)溫(wen)(wen)(wen)(wen)下(xia)(xia)(xia)使(shi)(shi)(shi)(shi)之全(quan)(quan)融(rong)(rong)。使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)前再預溫(wen)(wen)(wen)(wen)到將(jiang)(jiang)(jiang)(jiang)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)溫(wen)(wen)(wen)(wen)度(du)(du)(du)(如 37 ℃ ),但(dan)(dan)必(bi)(bi)須(xu)(xu)注意的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi),解(jie)(jie)(jie)(jie)凍(dong)(dong)過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)應(ying)輕(qing)輕(qing)地(di)(di)搖(yao)晃均(jun)(jun)勻。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)解(jie)(jie)(jie)(jie)凍(dong)(dong)過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)可能會(hui)(hui)(hui)(hui)(hui)出(chu)(chu)(chu)(chu)(chu)現(xian)少(shao)量(liang)(liang)(liang)(liang)(liang)絮(xu)(xu)狀(zhuang)(zhuang)(zhuang)(zhuang)或(huo)(huo)片狀(zhuang)(zhuang)(zhuang)(zhuang)析(xi)(xi)出(chu)(chu)(chu)(chu)(chu)物(wu)(wu)(wu)(wu)(wu)(wu)(wu),主(zhu)(zhu)要(yao)是(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)脂(zhi)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)變(bian)性(xing)或(huo)(huo)是(shi)(shi)(shi)(shi)纖(xian)(xian)維(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(形(xing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)凝(ning)(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)之一(yi)(yi)(yi))析(xi)(xi)出(chu)(chu)(chu)(chu)(chu),但(dan)(dan)這些(xie)(xie)(xie)析(xi)(xi)出(chu)(chu)(chu)(chu)(chu)物(wu)(wu)(wu)(wu)(wu)(wu)(wu),并(bing)不(bu)(bu)(bu)影(ying)(ying)(ying)(ying)響(xiang)(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本(ben)(ben)(ben)身的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)。 若(ruo)一(yi)(yi)(yi)次無(wu)(wu)法(fa)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)一(yi)(yi)(yi)瓶(ping),建議無(wu)(wu)菌分(fen)(fen)(fen)(fen)(fen)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)至(zhi)恰(qia)當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)滅菌容(rong)器內(nei),再放(fang)(fang)回冷(leng)凍(dong)(dong)。注意須(xu)(xu)知（僅供參(can)考(kao)）：1.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)凍(dong)(dong)采用(yong)(yong)(yong)(yong)(yong)(yong)(yong)逐步解(jie)(jie)(jie)(jie)凍(dong)(dong)法(fa)（-20℃→2—8℃→25℃或(huo)(huo)37℃），解(jie)(jie)(jie)(jie)凍(dong)(dong)過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)必(bi)(bi)須(xu)(xu)隨時輕(qing)搖(yao)，使(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)受熱(re)(re)溫(wen)(wen)(wen)(wen)度(du)(du)(du)均(jun)(jun)勻。2.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)凝(ning)(ning)(ning)(ning)(ning)絮(xu)(xu)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)凍(dong)(dong)后會(hui)(hui)(hui)(hui)(hui)出(chu)(chu)(chu)(chu)(chu)現(xian)少(shao)量(liang)(liang)(liang)(liang)(liang)片狀(zhuang)(zhuang)(zhuang)(zhuang)或(huo)(huo)絮(xu)(xu)狀(zhuang)(zhuang)(zhuang)(zhuang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)析(xi)(xi)出(chu)(chu)(chu)(chu)(chu)，這主(zhu)(zhu)要(yao)是(shi)(shi)(shi)(shi)由于(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)內(nei)不(bu)(bu)(bu)穩定蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)變(bian)性(xing)、纖(xian)(xian)維(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)析(xi)(xi)出(chu)(chu)(chu)(chu)(chu)形(xing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)沉(chen)(chen)(chen)(chen)淀物(wu)(wu)(wu)(wu)(wu)(wu)(wu)，實(shi)驗證(zheng)明(ming)沉(chen)(chen)(chen)(chen)淀物(wu)(wu)(wu)(wu)(wu)(wu)(wu)不(bu)(bu)(bu)會(hui)(hui)(hui)(hui)(hui)影(ying)(ying)(ying)(ying)響(xiang)(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本(ben)(ben)(ben)身質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)。3.熱(re)(re)滅活(huo)(huo)本(ben)(ben)(ben)產(chan)品除(chu)注明(ming)外均(jun)(jun)未(wei)(wei)滅活(huo)(huo)。如果(guo)(guo)必(bi)(bi)須(xu)(xu)滅活(huo)(huo)，請嚴格(ge)按照(zhao)56℃30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)（水浴）處理(li)(li)已(yi)解(jie)(jie)(jie)(jie)凍(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。4.常溫(wen)(wen)(wen)(wen)狀(zhuang)(zhuang)(zhuang)(zhuang)態下(xia)(xia)(xia)短期(qi)(qi)融(rong)(rong)化(hua)(hua)并(bing)不(bu)(bu)(bu)會(hui)(hui)(hui)(hui)(hui)影(ying)(ying)(ying)(ying)響(xiang)(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)。5.產(chan)品有(you)(you)(you)(you)效期(qi)(qi)，檢定合(he)格(ge)之日起(qi)(qi)(qi)有(you)(you)(you)(you)效期(qi)(qi)5年(nian)。鑒別(bie)(bie)方(fang)法(fa)： 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)凝(ning)(ning)(ning)(ning)(ning)結析(xi)(xi)出(chu)(chu)(chu)(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)淡黃色(se)通(tong)明(ming)液(ye)體(ti)(ti)。如將(jiang)(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)自血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管內(nei)抽(chou)出(chu)(chu)(chu)(chu)(chu)，放(fang)(fang)入(ru)(ru)試管中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，不(bu)(bu)(bu)加(jia)(jia)(jia)(jia)抗(kang)(kang)(kang)(kang)凝(ning)(ning)(ning)(ning)(ning)劑，則(ze)凝(ning)(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)響(xiang)(xiang)被激(ji)(ji)活(huo)(huo)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)敏捷凝(ning)(ning)(ning)(ning)(ning)結，構成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)膠凍(dong)(dong)。其(qi)(qi)周(zhou)圍(wei)所析(xi)(xi)出(chu)(chu)(chu)(chu)(chu)之淡黃色(se)通(tong)明(ming)液(ye)體(ti)(ti)即為(wei)(wei)(wei)(wei)(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，也可于(yu)(yu)凝(ning)(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后經(jing)離(li)心(xin)獲得(de)(de)。在(zai)凝(ning)(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，纖(xian)(xian)維(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)轉成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)為(wei)(wei)(wei)(wei)(wei)纖(xian)(xian)維(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)塊(kuai)，所以(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)無(wu)(wu)纖(xian)(xian)維(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)，這一(yi)(yi)(yi)點(dian)是(shi)(shi)(shi)(shi)與(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)zui大的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)差(cha)異。而(er)(er)(er)在(zai)凝(ning)(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)響(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)板(ban)開釋出(chu)(chu)(chu)(chu)(chu)很(hen)(hen)多(duo)(duo)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)，各(ge)也都(dou)發(fa)生(sheng)(sheng)(sheng)了(le)改(gai)(gai)變(bian)。這些(xie)(xie)(xie)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)都(dou)留在(zai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)并(bing)持續(xu)發(fa)生(sheng)(sheng)(sheng)改(gai)(gai)變(bian)，如原(yuan)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)為(wei)(wei)(wei)(wei)(wei)，并(bing)隨血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)寄(ji)存時刻逐步削減(jian)以(yi)致不(bu)(bu)(bu)見(jian)。這些(xie)(xie)(xie)也都(dou)是(shi)(shi)(shi)(shi)與(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)差(cha)異的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)地(di)(di)方(fang)。但(dan)(dan)很(hen)(hen)多(duo)(duo)未(wei)(wei)參(can)與(yu)凝(ning)(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)響(xiang)(xiang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)則(ze)與(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)根本(ben)(ben)(ben)一(yi)(yi)(yi)樣(yang)。為(wei)(wei)(wei)(wei)(wei)防止(zhi)(zhi)(zhi)抗(kang)(kang)(kang)(kang)凝(ning)(ning)(ning)(ning)(ning)劑的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)攪擾，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)很(hen)(hen)多(duo)(duo)化(hua)(hua)學成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)剖析(xi)(xi)，都(dou)以(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)(wei)(wei)(wei)(wei)樣(yang)品。 抗(kang)(kang)(kang)(kang)凝(ning)(ning)(ning)(ning)(ning)馬(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)要(yao)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)：●提供基本(ben)(ben)(ben)營(ying)養物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)：氨基酸(suan)(suan)、維(wei)(wei)(wei)(wei)生(sheng)(sheng)(sheng)素(su)(su)(su)、無(wu)(wu)機(ji)(ji)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)、脂(zhi)類物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)、核酸(suan)(suan)衍生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)等(deng)，是(shi)(shi)(shi)(shi)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)長(chang)(chang)必(bi)(bi)須(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)。●提供激(ji)(ji)素(su)(su)(su)和(he)(he)各(ge)種生(sheng)(sheng)(sheng)長(chang)(chang)因(yin)子(zi)：胰(yi)島素(su)(su)(su)、腎上(shang)腺皮質(zhi)(zhi)(zhi)(zhi)激(ji)(ji)素(su)(su)(su)（*******、地(di)(di)塞米松）、類固(gu)醇(chun)(chun)(chun)激(ji)(ji)素(su)(su)(su)（雌二(er)醇(chun)(chun)(chun)、睪酮、孕酮）等(deng)。生(sheng)(sheng)(sheng)長(chang)(chang)因(yin)子(zi)如成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)纖(xian)(xian)維(wei)(wei)(wei)(wei)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)長(chang)(chang)因(yin)子(zi)、表皮生(sheng)(sheng)(sheng)長(chang)(chang)因(yin)子(zi)、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)板(ban)生(sheng)(sheng)(sheng)長(chang)(chang)因(yin)子(zi)等(deng)。●提供結合(he)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)：結合(he)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)(shi)攜(xie)帶重(zhong)要(yao)地(di)(di)低分(fen)(fen)(fen)(fen)(fen)子(zi)量(liang)(liang)(liang)(liang)(liang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)，如白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜(xie)帶維(wei)(wei)(wei)(wei)生(sheng)(sheng)(sheng)素(su)(su)(su)、脂(zhi)肪、以(yi)及激(ji)(ji)素(su)(su)(su)等(deng)，轉鐵蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜(xie)帶鐵。結合(he)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)在(zai)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)代謝過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)起(qi)(qi)(qi)重(zhong)要(yao)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。●提供促(cu)接觸和(he)(he)伸展因(yin)子(zi)使(shi)(shi)(shi)(shi)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)壁免受機(ji)(ji)械(xie)損(sun)(sun)傷(shang)。●對(dui)培養中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)起(qi)(qi)(qi)到某些(xie)(xie)(xie)保護作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)：有(you)(you)(you)(you)一(yi)(yi)(yi)些(xie)(xie)(xie)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)，如內(nei)皮細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)、骨髓樣(yang)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)可以(yi)釋放(fang)(fang)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)含(han)(han)有(you)(you)(you)(you)抗(kang)(kang)(kang)(kang)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)，起(qi)(qi)(qi)到中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。這種作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)(shi)偶然發(fa)現(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，現(xian)在(zai)則(ze)有(you)(you)(you)(you)目的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)來終止(zhi)(zhi)(zhi)胰(yi)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消(xiao)化(hua)(hua)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。 因(yin)為(wei)(wei)(wei)(wei)(wei)胰(yi)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)已(yi)經(jing)被廣泛用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)貼(tie)壁細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消(xiao)化(hua)(hua)傳代。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)形(xing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)了(le)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)粘度(du)(du)(du)，可以(yi)保護細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)免受機(ji)(ji)械(xie)損(sun)(sun)傷(shang)，特(te)別(bie)(bie)是(shi)(shi)(shi)(shi)在(zai)懸(xuan)浮培養攪拌時，粘度(du)(du)(du)起(qi)(qi)(qi)到重(zhong)要(yao)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)還含(han)(han)有(you)(you)(you)(you)一(yi)(yi)(yi)些(xie)(xie)(xie)微量(liang)(liang)(liang)(liang)(liang)元素(su)(su)(su)和(he)(he)離(li)子(zi)，他們在(zai)代謝解(jie)(jie)(jie)(jie)毒(du)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)起(qi)(qi)(qi)重(zhong)要(yao)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如SeO3,硒等(deng)。抗(kang)(kang)(kang)(kang)凝(ning)(ning)(ning)(ning)(ning)馬(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)（無(wu)(wu)菌 袋裝(zhuang)）血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)要(yao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)使(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)出(chu)(chu)(chu)(chu)(chu)去纖(xian)(xian)維(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)而(er)(er)(er)形(xing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)種和(he)(he)復(fu)雜的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu),含(han)(han)有(you)(you)(you)(you)各(ge)種血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、多(duo)(duo)肽(tai)、脂(zhi)肪、碳水化(hua)(hua)合(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)長(chang)(chang)因(yin)子(zi)、激(ji)(ji)素(su)(su)(su)以(yi)及無(wu)(wu)機(ji)(ji)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)等(deng),具(ju)有(you)(you)(you)(you)維(wei)(wei)(wei)(wei)持血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正常粘度(du)(du)(du)、酸(suan)(suan)堿度(du)(du)(du)、滲透壓等(deng)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong),且(qie)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)及其(qi)(qi)含(han)(han)量(liang)(liang)(liang)(liang)(liang)常隨動物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)別(bie)(bie)、年(nian)齡、生(sheng)(sheng)(sheng)理(li)(li)條件(jian)和(he)(he)營(ying)養條件(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)不(bu)(bu)(bu)同而(er)(er)(er)異。它主(zhu)(zhu)要(yao)由水和(he)(he)各(ge)種化(hua)(hua)學成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)組成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng),包括白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、α1、α2、β、γ-球蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、甘油三酯、總膽(dan)固(gu)醇(chun)(chun)(chun)、谷丙轉氨酶(mei)等(deng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)保存應(ying)該(gai)注意以(yi)下(xia)(xia)(xia)幾點(dian)：(1)需(xu)要(yao)長(chang)(chang)期(qi)(qi)保存的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必(bi)(bi)須(xu)(xu)儲(chu)存于(yu)(yu)-20℃-70℃低溫(wen)(wen)(wen)(wen)冰(bing)箱(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong).4℃冰(bing)箱(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)保存時間(jian)切(qie)(qie)勿(wu)(wu)(wu)超(chao)過(guo)(guo)(guo)1個月(yue).由于(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結冰(bing)時體(ti)(ti)積(ji)會(hui)(hui)(hui)(hui)(hui)增加(jia)(jia)(jia)(jia)約(yue)10%,因(yin)此(ci),血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)凍(dong)(dong)入(ru)(ru)低溫(wen)(wen)(wen)(wen)冰(bing)箱(xiang)前,必(bi)(bi)須(xu)(xu)預留一(yi)(yi)(yi)定體(ti)(ti)積(ji)空間(jian),否(fou)則(ze)易發(fa)生(sheng)(sheng)(sheng)污染或(huo)(huo)玻璃瓶(ping)凍(dong)(dong)裂(lie).(2)熱(re)(re)滅活(huo)(huo)是(shi)(shi)(shi)(shi)指56℃,30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)加(jia)(jia)(jia)(jia)熱(re)(re)已(yi)*解(jie)(jie)(jie)(jie)凍(dong)(dong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).加(jia)(jia)(jia)(jia)熱(re)(re)過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)須(xu)(xu)規則(ze)搖(yao)晃均(jun)(jun)勻.此(ci)熱(re)(re)處理(li)(li)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)目的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)使(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補(bu)體(ti)(ti)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(complement)滅活(huo)(huo).除(chu)非必(bi)(bi)須(xu)(xu),一(yi)(yi)(yi)般(ban)不(bu)(bu)(bu)建議作(zuo)(zuo)(zuo)此(ci)熱(re)(re)處理(li)(li),因(yin)為(wei)(wei)(wei)(wei)(wei)熱(re)(re)處理(li)(li)會(hui)(hui)(hui)(hui)(hui)造成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)沉(chen)(chen)(chen)(chen)淀物(wu)(wu)(wu)(wu)(wu)(wu)(wu)顯著增多(duo)(duo),而(er)(er)(er)且(qie)還會(hui)(hui)(hui)(hui)(hui)影(ying)(ying)(ying)(ying)響(xiang)(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang).補(bu)體(ti)(ti)參(can)與(yu)反(fan)應(ying)有(you)(you)(you)(you):細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)毒(du)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong),平滑肌細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)收縮,肥大細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)和(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)板(ban)釋放(fang)(fang)組胺,增強吞噬(shi)(shi)(shi)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong),促(cu)進淋(lin)巴細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)和(he)(he)巨噬(shi)(shi)(shi)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)發(fa)生(sheng)(sheng)(sheng)化(hua)(hua)學趨化(hua)(hua)和(he)(he)活(huo)(huo)化(hua)(hua).(3)瓶(ping)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)凍(dong)(dong)需(xu)采用(yong)(yong)(yong)(yong)(yong)(yong)(yong)逐步解(jie)(jie)(jie)(jie)凍(dong)(dong)法(fa):-20℃至(zhi)-70℃低溫(wen)(wen)(wen)(wen)冰(bing)箱(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)放(fang)(fang)入(ru)(ru)4℃冰(bing)箱(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)溶(rong)解(jie)(jie)(jie)(jie)1天(tian).然后移入(ru)(ru)室(shi)(shi)溫(wen)(wen)(wen)(wen),待(dai)全(quan)(quan)部溶(rong)解(jie)(jie)(jie)(jie)后再分(fen)(fen)(fen)(fen)(fen)裝(zhuang).在(zai)溶(rong)解(jie)(jie)(jie)(jie)過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)需(xu)不(bu)(bu)(bu)斷輕(qing)輕(qing)搖(yao)晃均(jun)(jun)勻(小(xiao)(xiao)心(xin)勿(wu)(wu)(wu)造成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)氣泡),使(shi)(shi)(shi)(shi)溫(wen)(wen)(wen)(wen)度(du)(du)(du)與(yu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)均(jun)(jun)一(yi)(yi)(yi),減(jian)少(shao)沉(chen)(chen)(chen)(chen)淀的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)生(sheng)(sheng)(sheng).切(qie)(qie)勿(wu)(wu)(wu)直(zhi)接將(jiang)(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)-20℃進入(ru)(ru)37℃解(jie)(jie)(jie)(jie)凍(dong)(dong),這樣(yang)因(yin)溫(wen)(wen)(wen)(wen)度(du)(du)(du)改(gai)(gai)變(bian)太大,容(rong)易造成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)凝(ning)(ning)(ning)(ning)(ning)集而(er)(er)(er)出(chu)(chu)(chu)(chu)(chu)現(xian)沉(chen)(chen)(chen)(chen)淀.(4)一(yi)(yi)(yi)般(ban)廠商提供的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)(wei)(wei)(wei)(wei)無(wu)(wu)菌,無(wu)(wu)需(xu)再過(guo)(guo)(guo)濾除(chu)菌.如發(fa)現(xian)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)有(you)(you)(you)(you)懸(xuan)浮物(wu)(wu)(wu)(wu)(wu)(wu)(wu),則(ze)可將(jiang)(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)加(jia)(jia)(jia)(jia)入(ru)(ru)培養液(ye)內(nei)一(yi)(yi)(yi)起(qi)(qi)(qi)過(guo)(guo)(guo)濾,切(qie)(qie)勿(wu)(wu)(wu)直(zhi)接過(guo)(guo)(guo)濾血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).(5)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)沉(chen)(chen)(chen)(chen)淀物(wu)(wu)(wu)(wu)(wu)(wu)(wu)絮(xu)(xu)狀(zhuang)(zhuang)(zhuang)(zhuang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu):主(zhu)(zhu)要(yao)是(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂(zhi)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)變(bian)性(xing)及解(jie)(jie)(jie)(jie)凍(dong)(dong)后血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)纖(xian)(xian)維(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)造成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng),這些(xie)(xie)(xie)絮(xu)(xu)狀(zhuang)(zhuang)(zhuang)(zhuang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)不(bu)(bu)(bu)會(hui)(hui)(hui)(hui)(hui)影(ying)(ying)(ying)(ying)響(xiang)(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本(ben)(ben)(ben)身的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang).可用(yong)(yong)(yong)(yong)(yong)(yong)(yong)離(li)心(xin)3000rpm,5分(fen)(fen)(fen)(fen)(fen)鐘(zhong)去除(chu),也可不(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)處理(li)(li).顯微鏡下(xia)(xia)(xia)小(xiao)(xiao)黑(hei)點(dian):經(jing)過(guo)(guo)(guo)熱(re)(re)處理(li)(li)過(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),沉(chen)(chen)(chen)(chen)淀物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)形(xing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)會(hui)(hui)(hui)(hui)(hui)顯著增多(duo)(duo).有(you)(you)(you)(you)些(xie)(xie)(xie)沉(chen)(chen)(chen)(chen)淀物(wu)(wu)(wu)(wu)(wu)(wu)(wu)在(zai)顯微鏡下(xia)(xia)(xia)觀察象小(xiao)(xiao)黑(hei)點(dian),常誤(wu)認(ren)為(wei)(wei)(wei)(wei)(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)受污染.一(yi)(yi)(yi)般(ban)情況下(xia)(xia)(xia),此(ci)小(xiao)(xiao)黑(hei)點(dian)不(bu)(bu)(bu)會(hui)(hui)(hui)(hui)(hui)影(ying)(ying)(ying)(ying)響(xiang)(xiang)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)長(chang)(chang),但(dan)(dan)如果(guo)(guo)懷(huai)疑血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang),則(ze)應(ying)立即停止(zhi)(zhi)(zhi)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong),更換(huan)另一(yi)(yi)(yi)批號的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).(6)切(qie)(qie)勿(wu)(wu)(wu)將(jiang)(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)37℃放(fang)(fang)置(zhi)太久,否(fou)則(ze)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)會(hui)(hui)(hui)(hui)(hui)變(bian)得(de)(de)渾(hun)濁,同時血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)(you)(you)效成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)會(hui)(hui)(hui)(hui)(hui)破會(hui)(hui)(hui)(hui)(hui)而(er)(er)(er)影(ying)(ying)(ying)(ying)響(xiang)(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang).公司(si)產(chan)品僅用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)科研對(dui)于(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)研究,仍然存在(zai)一(yi)(yi)(yi)些(xie)(xie)(xie)未(wei)(wei)解(jie)(jie)(jie)(jie)之謎(mi):1.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)復(fu)雜,可能有(you)(you)(you)(you)幾百種之多(duo)(duo),對(dui)其(qi)(qi)準(zhun)確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)、含(han)(han)量(liang)(liang)(liang)(liang)(liang)及作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)尚且(qie)不(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),尤其(qi)(qi)是(shi)(shi)(shi)(shi)一(yi)(yi)(yi)些(xie)(xie)(xie)多(duo)(duo)肽(tai)生(sheng)(sheng)(sheng)長(chang)(chang)類因(yin)子(zi)、激(ji)(ji)素(su)(su)(su)和(he)(he)脂(zhi)類等(deng)。2.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都(dou)是(shi)(shi)(shi)(shi)批量(liang)(liang)(liang)(liang)(liang) ,各(ge)批量(liang)(liang)(liang)(liang)(liang)之間(jian)差(cha)異很(hen)(hen)大,且(qie)保存期(qi)(qi)一(yi)(yi)(yi)般(ban)不(bu)(bu)(bu)超(chao)過(guo)(guo)(guo)一(yi)(yi)(yi)年(nian),因(yin)此(ci)要(yao)保證(zheng)每批血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)相似性(xing)幾乎(hu)不(bu)(bu)(bu)太現(xian)實(shi);3.不(bu)(bu)(bu)能排除(chu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)含(han)(han)有(you)(you)(you)(you)易變(bian)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi),這被認(ren)為(wei)(wei)(wei)(wei)(wei)是(shi)(shi)(shi)(shi)“瓶(ping)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)”的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)因(yin)之一(yi)(yi)(yi)。 質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)標準(zhun)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)高低取(qu)(qu)決于(yu)(yu)兩方(fang)面因(yin)素(su)(su)(su)：一(yi)(yi)(yi)是(shi)(shi)(shi)(shi)取(qu)(qu)材(cai)對(dui)象，二(er)是(shi)(shi)(shi)(shi)取(qu)(qu)材(cai)過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)取(qu)(qu)材(cai)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)動物(wu)(wu)(wu)(wu)(wu)(wu)(wu)應(ying)健康無(wu)(wu)病并(bing)且(qie)在(zai)指定的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)出(chu)(chu)(chu)(chu)(chu)生(sheng)(sheng)(sheng)天(tian)數之內(nei)，取(qu)(qu)材(cai)過(guo)(guo)(guo)程(cheng)(cheng)(cheng)(cheng)應(ying)嚴格(ge)按照(zhao)操作(zuo)(zuo)(zuo)規程(cheng)(cheng)(cheng)(cheng)執行，制備出(chu)(chu)(chu)(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)要(yao)經(jing)過(guo)(guo)(guo)嚴格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)鑒定。1． 牛(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必(bi)(bi)須(xu)(xu)來自有(you)(you)(you)(you)文件(jian)證(zheng)明(ming)無(wu)(wu)牛(niu)(niu)海綿狀(zhuang)(zhuang)(zhuang)(zhuang)腦病的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)牛(niu)(niu)群(qun)或(huo)(huo)國家。并(bing)應(ying)具(ju)備適當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)監測系(xi)統。2． 有(you)(you)(you)(you)些(xie)(xie)(xie)國家還要(yao)求牛(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)來自未(wei)(wei)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)過(guo)(guo)(guo)反(fan)芻動物(wu)(wu)(wu)(wu)(wu)(wu)(wu)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)飼料的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)牛(niu)(niu)群(qun)。3． 證(zheng)明(ming)所用(yong)(yong)(yong)(yong)(yong)(yong)(yong)牛(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)不(bu)(bu)(bu)含(han)(han)對(dui)所生(sheng)(sheng)(sheng)產(chan)疫苗(miao)病毒(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)抑制物(wu)(wu)(wu)(wu)(wu)(wu)(wu)。4． 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)要(yao)通(tong)過(guo)(guo)(guo)濾膜過(guo)(guo)(guo)濾除(chu)菌，保證(zheng)無(wu)(wu)菌。5． 無(wu)(wu)細(xi)(xi)(xi)菌、霉菌、支(zhi)原(yuan)體(ti)(ti)和(he)(he)病毒(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)污染，有(you)(you)(you)(you)些(xie)(xie)(xie)國家要(yao)求無(wu)(wu)細(xi)(xi)(xi)菌噬(shi)(shi)(shi)菌體(ti)(ti)污染。6． 對(dui)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)有(you)(you)(you)(you)良好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)支(zhi)持繁殖作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。
抗(kang)凝(ning)馬血(xue)(xue)（無(wu)菌(jun) pet瓶(ping)裝(zhuang)）用(yong)(yong)(yong)法(fa)(fa) 血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)由(you)冰(bing)凍(dong)(dong)(dong)(dong)狀(zhuang)態在常(chang)(chang)溫(wen)(wen)下(xia)完全(quan)融(rong)化(hua)(hua)(hua)成(cheng)(cheng)液(ye)體(ti),搖(yao)(yao)均(jun)勻后(hou)(hou)放置 10-30 分(fen)鐘(zhong)待結(jie)塊(kuai)蛋白(bai)(bai)質(zhi)(zhi)沉底后(hou)(hou)即可(ke) 使(shi)用(yong)(yong)(yong)。注(zhu)意(yi)事項 有少(shao)量(liang)蛋白(bai)(bai)質(zhi)(zhi)結(jie)塊(kuai)析(xi)出(chu)(chu)(chu)不(bu)影(ying)(ying)響(xiang)使(shi)用(yong)(yong)(yong)。未融(rong)化(hua)(hua)(hua)或融(rong)化(hua)(hua)(hua)未經搖(yao)(yao)勻的(de)(de)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)禁止直接放入(ru)高溫(wen)(wen)水浴內加(jia)溫(wen)(wen)。減少(shao)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)重復凍(dong)(dong)(dong)(dong)融(rong)。血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)在室溫(wen)(wen)或 4 ℃ 冰(bing)箱內放置過久會(hui)(hui)(hui)影(ying)(ying)響(xiang)促細(xi)胞生長效果。抗(kang)凝(ning)馬血(xue)(xue)(無(wu)菌(jun) 袋裝(zhuang)) 的(de)(de)解(jie)(jie)凍(dong)(dong)(dong)(dong): 將(jiang)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)從冰(bing)凍(dong)(dong)(dong)(dong)區(qu)中(zhong)(zhong)取出(chu)(chu)(chu)后(hou)(hou)(切勿直接將(jiang)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)從 -20 ℃ 進入(ru) 37 ℃ 解(jie)(jie)凍(dong)(dong)(dong)(dong),這(zhe)樣因溫(wen)(wen)度改變太大,容易造(zao)成(cheng)(cheng)蛋白(bai)(bai)質(zhi)(zhi)凝(ning)集而出(chu)(chu)(chu)現(xian)沉淀(dian)),先置于 2 ~ 8 ℃ 區(qu)域中(zhong)(zhong)使(shi)之(zhi)(zhi)融(rong)化(hua)(hua)(hua),然后(hou)(hou)在室溫(wen)(wen)下(xia)使(shi)之(zhi)(zhi)全(quan)融(rong)。使(shi)用(yong)(yong)(yong)前再(zai)預溫(wen)(wen)到將(jiang)使(shi)用(yong)(yong)(yong)的(de)(de)溫(wen)(wen)度(如 37 ℃ ),但(dan)必(bi)須(xu)注(zhu)意(yi)的(de)(de)是(shi),解(jie)(jie)凍(dong)(dong)(dong)(dong)過程中(zhong)(zhong)應輕輕地(di)搖(yao)(yao)晃均(jun)勻。血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)在解(jie)(jie)凍(dong)(dong)(dong)(dong)過程中(zhong)(zhong)可(ke)能會(hui)(hui)(hui)出(chu)(chu)(chu)現(xian)少(shao)量(liang)絮(xu)狀(zhuang)或片(pian)狀(zhuang)析(xi)出(chu)(chu)(chu)物,主(zhu)要(yao)是(shi)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)脂蛋白(bai)(bai)的(de)(de)變性或是(shi)纖(xian)維蛋白(bai)(bai)(形(xing)成(cheng)(cheng)凝(ning)血(xue)(xue)的(de)(de)蛋白(bai)(bai)之(zhi)(zhi)一(yi)(yi))析(xi)出(chu)(chu)(chu),但(dan)這(zhe)些析(xi)出(chu)(chu)(chu)物,并(bing)不(bu)影(ying)(ying)響(xiang)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)本身的(de)(de)質(zhi)(zhi)量(liang)。 若一(yi)(yi)次無(wu)法(fa)(fa)用(yong)(yong)(yong)完一(yi)(yi)瓶(ping),建議無(wu)菌(jun)分(fen)裝(zhuang)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)至(zhi)恰當的(de)(de)滅(mie)菌(jun)容器(qi)內,再(zai)放回冷(leng)凍(dong)(dong)(dong)(dong)。注(zhu)意(yi)須(xu)知(zhi)（僅供參考）： 1.血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)凍(dong)(dong)(dong)(dong)采用(yong)(yong)(yong)逐步解(jie)(jie)凍(dong)(dong)(dong)(dong)法(fa)(fa)（-20℃→2—8℃→25℃或37℃），解(jie)(jie)凍(dong)(dong)(dong)(dong)過程中(zhong)(zhong)必(bi)須(xu)隨時輕搖(yao)(yao)，使(shi)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)受熱溫(wen)(wen)度均(jun)勻。 2.血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)凝(ning)絮(xu)物，血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)凍(dong)(dong)(dong)(dong)后(hou)(hou)會(hui)(hui)(hui)出(chu)(chu)(chu)現(xian)少(shao)量(liang)片(pian)狀(zhuang)或絮(xu)狀(zhuang)物析(xi)出(chu)(chu)(chu)，這(zhe)主(zhu)要(yao)是(shi)由(you)于血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)內不(bu)穩定蛋白(bai)(bai)變性、纖(xian)維蛋白(bai)(bai)析(xi)出(chu)(chu)(chu)形(xing)成(cheng)(cheng)沉淀(dian)物，實驗證明沉淀(dian)物不(bu)會(hui)(hui)(hui)影(ying)(ying)響(xiang)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)本身質(zhi)(zhi)量(liang)。 3.熱滅(mie)活(huo)本產(chan)品(pin)除注(zhu)明外均(jun)未滅(mie)活(huo)。如果必(bi)須(xu)滅(mie)活(huo)，請嚴格按(an)照(zhao)56℃30分(fen)鐘(zhong)（水浴）處理(li)已(yi)解(jie)(jie)凍(dong)(dong)(dong)(dong)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)。 4.常(chang)(chang)溫(wen)(wen)狀(zhuang)態下(xia)短期融(rong)化(hua)(hua)(hua)并(bing)不(bu)會(hui)(hui)(hui)影(ying)(ying)響(xiang)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)量(liang)。 5.產(chan)品(pin)有效期，檢定合格之(zhi)(zhi)日(ri)起(qi)有效期5年。 鑒別方法(fa)(fa)： 血(xue)(xue)液(ye)凝(ning)結(jie)析(xi)出(chu)(chu)(chu)的(de)(de)淡黃色通明液(ye)體(ti)。如將(jiang)血(xue)(xue)液(ye)自(zi)血(xue)(xue)管(guan)內抽出(chu)(chu)(chu)，放入(ru)試管(guan)中(zhong)(zhong)，不(bu)加(jia)抗(kang)凝(ning)劑，則(ze)凝(ning)血(xue)(xue)反響(xiang)被激活(huo)，血(xue)(xue)液(ye)敏捷凝(ning)結(jie)，構成(cheng)(cheng)膠凍(dong)(dong)(dong)(dong)。其(qi)周圍所析(xi)出(chu)(chu)(chu)之(zhi)(zhi)淡黃色通明液(ye)體(ti)即為血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)，也可(ke)于凝(ning)血(xue)(xue)后(hou)(hou)經離心獲得。在凝(ning)血(xue)(xue)過程中(zhong)(zhong)，纖(xian)維蛋白(bai)(bai)原轉成(cheng)(cheng)為纖(xian)維蛋白(bai)(bai)塊(kuai)，所以血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)無(wu)纖(xian)維蛋白(bai)(bai)原，這(zhe)一(yi)(yi)點是(shi)與血(xue)(xue)漿zui大的(de)(de)差異。而在凝(ning)血(xue)(xue)反響(xiang)中(zhong)(zhong)，血(xue)(xue)小板開釋出(chu)(chu)(chu)很多物質(zhi)(zhi)，各也都發生了改變。這(zhe)些成(cheng)(cheng)分(fen)都留在血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)并(bing)持續發生改變，如原成(cheng)(cheng)為，并(bing)隨血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)寄存時刻逐步削減以致不(bu)見。這(zhe)些也都是(shi)與血(xue)(xue)漿差異的(de)(de)地(di)方。但(dan)很多未參與凝(ning)血(xue)(xue)反響(xiang)的(de)(de)物質(zhi)(zhi)則(ze)與血(xue)(xue)漿根本一(yi)(yi)樣。為防止抗(kang)凝(ning)劑的(de)(de)攪擾，血(xue)(xue)液(ye)中(zhong)(zhong)很多化(hua)(hua)(hua)學成(cheng)(cheng)分(fen)的(de)(de)剖析(xi)，都以血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)為樣品(pin)。 抗(kang)凝(ning)馬血(xue)(xue)的(de)(de)主(zhu)要(yao)作用(yong)(yong)(yong)：●提供基本營養物質(zhi)(zhi)：氨基酸、維生素、無(wu)機物、脂類物質(zhi)(zhi)、核酸衍生物等，是(shi)細(xi)胞生長必(bi)須(xu)的(de)(de)物質(zhi)(zhi)。●提供激素和各種生長因子：胰島素、腎上腺皮質(zhi)(zhi)激素（*******、地(di)塞米松）、類固醇激素（雌二醇、睪酮、孕酮）等。生長因子如成(cheng)(cheng)纖(xian)維細(xi)胞生長因子、表皮生長因子、血(xue)(xue)小板生長因子等。●提供結(jie)合蛋白(bai)(bai)：結(jie)合蛋白(bai)(bai)作用(yong)(yong)(yong)是(shi)攜帶重要(yao)地(di)低分(fen)子量(liang)物質(zhi)(zhi)，如白(bai)(bai)蛋白(bai)(bai)攜帶維生素、脂肪、以及激素等，轉鐵蛋白(bai)(bai)攜帶鐵。結(jie)合蛋白(bai)(bai)在細(xi)胞代謝過程中(zhong)(zhong)起(qi)重要(yao)作用(yong)(yong)(yong)。●提供促接觸和伸展因子使(shi)細(xi)胞貼壁免受機械損傷。●對培養中(zhong)(zhong)的(de)(de)細(xi)胞起(qi)到某些保護作用(yong)(yong)(yong)：有一(yi)(yi)些細(xi)胞，如內皮細(xi)胞、骨髓樣細(xi)胞可(ke)以釋放蛋白(bai)(bai)酶，血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)含有抗(kang)蛋白(bai)(bai)酶成(cheng)(cheng)分(fen)，起(qi)到中(zhong)(zhong)和作用(yong)(yong)(yong)。這(zhe)種作用(yong)(yong)(yong)是(shi)偶然發現(xian)的(de)(de)，現(xian)在則(ze)有目的(de)(de)的(de)(de)使(shi)用(yong)(yong)(yong)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)來終止胰蛋白(bai)(bai)酶的(de)(de)消化(hua)(hua)(hua)作用(yong)(yong)(yong)。 因為胰蛋白(bai)(bai)酶已(yi)經被廣泛用(yong)(yong)(yong)于貼壁細(xi)胞的(de)(de)消化(hua)(hua)(hua)傳代。血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)蛋白(bai)(bai)形(xing)成(cheng)(cheng)了血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)粘度，可(ke)以保護細(xi)胞免受機械損傷，特別是(shi)在懸浮培養攪拌時，粘度起(qi)到重要(yao)作用(yong)(yong)(yong)。血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)還含有一(yi)(yi)些微量(liang)元素和離子，他們在代謝解(jie)(jie)毒中(zhong)(zhong)起(qi)重要(yao)作用(yong)(yong)(yong)，如SeO3,硒等。抗(kang)凝(ning)馬血(xue)(xue)（無(wu)菌(jun) pet瓶(ping)裝(zhuang)）血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)主(zhu)要(yao)成(cheng)(cheng)分(fen) 血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)使(shi)血(xue)(xue)漿出(chu)(chu)(chu)去纖(xian)維蛋白(bai)(bai)原而形(xing)成(cheng)(cheng)的(de)(de)一(yi)(yi)種和復雜的(de)(de)混合物,含有各種血(xue)(xue)漿蛋白(bai)(bai)、多肽、脂肪、碳水化(hua)(hua)(hua)合物、生長因子、激素以及無(wu)機物等,具有維持血(xue)(xue)液(ye)的(de)(de)正常(chang)(chang)粘度、酸堿度、滲透壓等作用(yong)(yong)(yong),且血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)成(cheng)(cheng)分(fen)及其(qi)含量(liang)常(chang)(chang)隨動物的(de)(de)性別、年齡、生理(li)條件和營養條件的(de)(de)不(bu)同而異。它主(zhu)要(yao)由(you)水和各種化(hua)(hua)(hua)學成(cheng)(cheng)分(fen)組成(cheng)(cheng),包括白(bai)(bai)蛋白(bai)(bai)、α1、α2、β、γ-球蛋白(bai)(bai)、甘油三酯、總膽固醇、谷丙轉氨酶等。血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)保存應該注(zhu)意(yi)以下(xia)幾點： (1)需要(yao)長期保存的(de)(de)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)必(bi)須(xu)儲存于-20℃-70℃低溫(wen)(wen)冰(bing)箱中(zhong)(zhong).4℃冰(bing)箱中(zhong)(zhong)保存時間切勿超過1個月.由(you)于血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)結(jie)冰(bing)時體(ti)積會(hui)(hui)(hui)增加(jia)約10%,因此,血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)在凍(dong)(dong)(dong)(dong)入(ru)低溫(wen)(wen)冰(bing)箱前,必(bi)須(xu)預留一(yi)(yi)定體(ti)積空間,否則(ze)易發生污染或玻璃瓶(ping)凍(dong)(dong)(dong)(dong)裂. (2)熱滅(mie)活(huo)是(shi)指56℃,30分(fen)鐘(zhong)加(jia)熱已(yi)*解(jie)(jie)凍(dong)(dong)(dong)(dong)的(de)(de)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing).加(jia)熱過程中(zhong)(zhong)須(xu)規則(ze)搖(yao)(yao)晃均(jun)勻.此熱處理(li)的(de)(de)目的(de)(de)是(shi)使(shi)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)的(de)(de)補體(ti)成(cheng)(cheng)分(fen)(complement)滅(mie)活(huo).除非必(bi)須(xu),一(yi)(yi)般不(bu)建議作此熱處理(li),因為熱處理(li)會(hui)(hui)(hui)造(zao)成(cheng)(cheng)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)沉淀(dian)物顯著增多,而且還會(hui)(hui)(hui)影(ying)(ying)響(xiang)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)質(zhi)(zhi)量(liang).補體(ti)參與反應有:細(xi)胞毒作用(yong)(yong)(yong),平滑肌細(xi)胞收縮,肥大細(xi)胞和血(xue)(xue)小板釋放組胺,增強吞噬作用(yong)(yong)(yong),促進淋巴細(xi)胞和巨噬細(xi)胞發生化(hua)(hua)(hua)學趨化(hua)(hua)(hua)和活(huo)化(hua)(hua)(hua). (3)瓶(ping)裝(zhuang)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)凍(dong)(dong)(dong)(dong)需采用(yong)(yong)(yong)逐步解(jie)(jie)凍(dong)(dong)(dong)(dong)法(fa)(fa):-20℃至(zhi)-70℃低溫(wen)(wen)冰(bing)箱中(zhong)(zhong)的(de)(de)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)放入(ru)4℃冰(bing)箱中(zhong)(zhong)溶解(jie)(jie)1天.然后(hou)(hou)移入(ru)室溫(wen)(wen),待全(quan)部溶解(jie)(jie)后(hou)(hou)再(zai)分(fen)裝(zhuang).在溶解(jie)(jie)過程中(zhong)(zhong)需不(bu)斷輕輕搖(yao)(yao)晃均(jun)勻(小心勿造(zao)成(cheng)(cheng)氣泡),使(shi)溫(wen)(wen)度與成(cheng)(cheng)分(fen)均(jun)一(yi)(yi),減少(shao)沉淀(dian)的(de)(de)發生.切勿直接將(jiang)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)從-20℃進入(ru)37℃解(jie)(jie)凍(dong)(dong)(dong)(dong),這(zhe)樣因溫(wen)(wen)度改變太大,容易造(zao)成(cheng)(cheng)蛋白(bai)(bai)質(zhi)(zhi)凝(ning)集而出(chu)(chu)(chu)現(xian)沉淀(dian). (4)一(yi)(yi)般廠商提供的(de)(de)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)為無(wu)菌(jun),無(wu)需再(zai)過濾除菌(jun).如發現(xian)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)有懸浮物,則(ze)可(ke)將(jiang)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)加(jia)入(ru)培養液(ye)內一(yi)(yi)起(qi)過濾,切勿直接過濾血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing). (5)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)的(de)(de)沉淀(dian)物絮(xu)狀(zhuang)物:主(zhu)要(yao)是(shi)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)的(de)(de)脂蛋白(bai)(bai)變性及解(jie)(jie)凍(dong)(dong)(dong)(dong)后(hou)(hou)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)纖(xian)維蛋白(bai)(bai)造(zao)成(cheng)(cheng),這(zhe)些絮(xu)狀(zhuang)物不(bu)會(hui)(hui)(hui)影(ying)(ying)響(xiang)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)本身的(de)(de)質(zhi)(zhi)量(liang).可(ke)用(yong)(yong)(yong)離心3000rpm,5分(fen)鐘(zhong)去除,也可(ke)不(bu)用(yong)(yong)(yong)處理(li).顯微鏡下(xia)小黑點:經過熱處理(li)過的(de)(de)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing),沉淀(dian)物的(de)(de)形(xing)成(cheng)(cheng)會(hui)(hui)(hui)顯著增多.有些沉淀(dian)物在顯微鏡下(xia)觀察象小黑點,常(chang)(chang)誤認為血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)受污染.一(yi)(yi)般情況下(xia),此小黑點不(bu)會(hui)(hui)(hui)影(ying)(ying)響(xiang)細(xi)胞生長,但(dan)如果懷疑血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)量(liang),則(ze)應立即停止使(shi)用(yong)(yong)(yong),更換另一(yi)(yi)批號的(de)(de)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing). (6)切勿將(jiang)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)在37℃放置太久,否則(ze)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)會(hui)(hui)(hui)變得渾濁,同時血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)的(de)(de)有效成(cheng)(cheng)分(fen)會(hui)(hui)(hui)破會(hui)(hui)(hui)而影(ying)(ying)響(xiang)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)量(liang).公司產(chan)品(pin)僅用(yong)(yong)(yong)于科研 對于血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)成(cheng)(cheng)分(fen)的(de)(de)研究,仍然存在一(yi)(yi)些未解(jie)(jie)之(zhi)(zhi)謎: 1.血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)成(cheng)(cheng)分(fen)復雜,可(ke)能有幾百種之(zhi)(zhi)多,對其(qi)準確的(de)(de)成(cheng)(cheng)分(fen)、含量(liang)及作用(yong)(yong)(yong)尚且不(bu)清(qing)(qing)(qing)(qing)(qing)(qing),尤其(qi)是(shi)一(yi)(yi)些多肽生長類因子、激素和脂類等。 2.血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)都是(shi)批量(liang) ,各批量(liang)之(zhi)(zhi)間差異很大,且保存期一(yi)(yi)般不(bu)超過一(yi)(yi)年,因此要(yao)保證每批血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)相似性幾乎不(bu)太現(xian)實; 3.不(bu)能排除血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)含有易變物質(zhi)(zhi),這(zhe)被認為是(shi)“瓶(ping)中(zhong)(zhong)”的(de)(de)原因之(zhi)(zhi)一(yi)(yi)。 質(zhi)(zhi)量(liang)標準血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)量(liang)高低取決于兩方面因素：一(yi)(yi)是(shi)取材對象，二是(shi)取材過程。用(yong)(yong)(yong)于取材的(de)(de)動物應健康無(wu)病并(bing)且在指定的(de)(de)出(chu)(chu)(chu)生天數之(zhi)(zhi)內，取材過程應嚴格按(an)照(zhao)操作規程執行，制備出(chu)(chu)(chu)的(de)(de)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)要(yao)經過嚴格的(de)(de)質(zhi)(zhi)量(liang)鑒定。1． 牛血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)必(bi)須(xu)來自(zi)有文件證明無(wu)牛海綿狀(zhuang)腦病的(de)(de)牛群或國家。并(bing)應具備適當的(de)(de)監測系統。2． 有些國家還要(yao)求牛血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)來自(zi)未用(yong)(yong)(yong)過反芻動物蛋白(bai)(bai)飼料的(de)(de)牛群。3． 證明所用(yong)(yong)(yong)牛血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)不(bu)含對所生產(chan)疫苗病毒的(de)(de)抑制物。4． 血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)要(yao)通過濾膜過濾除菌(jun)，保證無(wu)菌(jun)。5． 無(wu)細(xi)菌(jun)、霉菌(jun)、支原體(ti)和病毒的(de)(de)污染，有些國家要(yao)求無(wu)細(xi)菌(jun)噬菌(jun)體(ti)污染。6． 對細(xi)胞有良好的(de)(de)支持繁殖作用(yong)(yong)(yong)。
抗凝綿羊血（無菌 袋裝） 注意事項: 盡管經測試Annexin V-FITC反復凍融5次對于其檢測效果無顯著影響,但為取得良好的使用效果,3-6個月內推薦4℃保存,并適當注意避免反復凍融。 如果有細菌或真菌污染,會嚴重影響檢測效果。 染色后宜盡快檢測,時間過長可能會導致凋亡或壞死細胞的數量增加。 如果細胞收集過程中使用了胰酶,需注意設法去除殘留的胰酶。殘留的胰酶會消化并降解Annexin V-FITC,導致染色失敗。 熒光物質均易發生淬滅,在進行熒光觀察時,盡量縮短觀察時間,同時在操作和存放過程中也盡量注意避光保存。 用于流式細胞儀檢測時,如果發現Annexin V-FITC單獨染色時出現了過多的PI假陽性細胞,并且通過調整相關設置和參數也無法改善,可以用PBS將Annexin V-FITC稀釋3-10倍后再進行檢測,這樣通常可以有效減少假陽性的壞死細胞。 需自備PBS。 本產品僅限于專業人員的科學研究用,不得用于臨床診斷或,不得用于食品或藥品,不得存放于普通住宅內。 為了您的安全和健康,請穿實驗服并戴一次性手套操作。操作步驟(僅供參考):1、貼壁細胞的消化①吸除培養液,用無菌 PBS、Hanks 液或無血清培養液洗滌細胞一次,以去除殘余的血清。②加入少量 Trypsin-EDTA solution,略蓋過細胞即可,室溫放置 0.5~2min, 不同的細胞消化時間有所不同。③顯微鏡下觀察,細胞明顯收縮,并且肉眼觀察培養器皿底部發現細胞的形態發生明顯的變化;或者用槍吹打細胞發現細胞剛好可以被吹打下來,吸除胰酶細胞消化液。加入含血清的 完全細胞培養液,吹打下細胞,即可直接用于后續實驗。④如果發現消化不足,則加入 Trypsin-EDTA solution 重新消化。⑤如果發現細胞消化時間過長,未及吹打細胞,細胞已經有部分直接從培養器皿底部脫落, 直接用胰酶細胞培養液把細胞全部吹打下來。1000~2000g 離心 1min,沉淀細胞,盡量去除胰酶細胞消化液后,加入含血清的完全培養液重新懸浮細胞,即可用于后續實驗。2、組織的消化不同的組織需要消化的時間相差很大,通常以消化后可以充分打散組織為宜。鑒別方法： 血液凝結析出的淡黃色通明液體。如將血液自血管內抽出，放入試管中，不加抗凝劑，則凝血反響被激活，血液敏捷凝結，構成膠凍。其周圍所析出之淡黃色通明液體即為血清，也可于凝血后經離心獲得。在凝血過程中，纖維蛋白原轉成為纖維蛋白塊，所以血清中無纖維蛋白原，這一點是與血漿zui大的差異。而在凝血反響中，血小板開釋出很多物質，各也都發生了改變。 這些成分都留在血清中并持續發生改變，如原成為，并隨血清寄存時刻逐步削減以致不見。這些也都是與血漿差異的地方。但很多未參與凝血反響的物質則與血漿根本一樣。為防止抗凝劑的攪擾，血液中很多化學成分的剖析，都以血清為樣品。血清保存應該注意以下幾點：(1)需要長期保存的血清必須儲存于-20℃-70℃低溫冰箱中.4℃冰箱中保存時間切勿超過1個月.由于血清結冰時體積會增加約10%,因此,血清在凍入低溫冰箱前,必須預留一定體積空間,否則易發生污染或玻璃瓶凍裂.(2)熱滅活是指56℃,30分鐘加熱已*解凍的血清.加熱過程中須規則搖晃均勻.此熱處理的目的是使血清中的補體成分(complement)滅活.除非必須,一般不建議作此熱處理,因為熱處理會造成血清沉淀物顯著增多,而且還會影響血清的質量.補體參與反應有:細胞毒作用,平滑肌細胞收縮,肥大細胞和血小板釋放組胺,增強吞噬作用,促進淋巴細胞和巨噬細胞發生化學趨化和活化.(3)瓶裝血清解凍需采用逐步解凍法:-20℃至-70℃低溫冰箱中的血清放入4℃冰箱中溶解1天.然后移入室溫,待全部溶解后再分裝.在溶解過程中需不斷輕輕搖晃均勻(小心勿造成氣泡),使溫度與成分均一,減少沉淀的發生.切勿直接將血清從-20℃進入37℃解凍,這樣因溫度改變太大,容易造成蛋白質凝集而出現沉淀.(4)一般廠商提供的血清為無菌,無需再過濾除菌.如發現血清有懸浮物,則可將血清加入培養液內一起過濾,切勿直接過濾血清.(5)血清中的沉淀物絮狀物:主要是血清中的脂蛋白變性及解凍后血清中纖維蛋白造成,這些絮狀物不會影響血清本身的質量.可用離心3000rpm,5分鐘去除,也可不用處理.顯微鏡下小黑點:經過熱處理過的血清,沉淀物的形成會顯著增多.有些沉淀物在顯微鏡下觀察象小黑點,常誤認為血清受污染.一般情況下,此小黑點不會影響細胞生長,但如果懷疑血清質量,則應立即停止使用,更換另一批號的血清.(6)切勿將血清在37℃放置太久,否則血清會變得渾濁,同時血清中的有效成分會破會而影響血清質量.抗凝綿羊血（無菌 袋裝） 用法 血清由冰凍狀態在常溫下完全融化成液體,搖均勻后放置 10-30 分鐘待結塊蛋白質沉底后即可使用。注意事項 有少量蛋白質結塊析出不影響使用。未融化或融化未經搖勻的血清禁止直接放入高溫水浴內加溫。減少血清重復凍融。血清在室溫或 4 ℃ 冰箱內放置過久會影響促細胞生長效果。解凍 將血清從冰凍區中取出后(切勿直接將血清從 -20 ℃ 進入 37 ℃ 解凍,這樣因溫度改變太大,容易造成蛋白質凝集而出現沉淀),先置于 2 ~ 8 ℃ 區域中使之融化,然后在室溫下使之全融。使用前再預溫到將使用的溫度(如 37 ℃ ),但必須注意的是,解凍過程中應輕輕地搖晃均勻。血清在解凍過程中可能會出現少量絮狀或片狀析出物,主要是血清中脂蛋白的變性或是纖維蛋白(形成凝血的蛋白之一)析出,但這些析出物,并不影響血清本身的質量。 若一次無法用完一瓶,建議無菌分裝血清至恰當的滅菌容器內,再放回冷凍。細胞培養的優點:1.研究的對象是活細胞在實驗過程中,根據要求可始終保持細胞活力,并可長時間監控、檢測甚至定量評估一部分活細胞的情況,包括活細胞的形態、結構、生命活動等。2.研究條件可以人為控制pH、溫度、氧氣、二氧化碳、張力等物理化學的條件,可以根據實際需要進行人為的控制,同時,可以施加化學、物理、生物等因素作為條件而進行實驗觀察,這些因素同樣可以處于嚴格控制之下。3.研究的樣本可以達到比較均一性通過細胞培養一定代數后,所得到的細胞系則可以達到均一性而屬于同一類型的細胞,需要時,可采用克隆化等方法使細胞達到純化。4.研究內容便于觀察、檢測和記錄采用各種研究技術:如倒置生物顯微鏡、熒光顯微鏡、電子顯微鏡、流式細胞術、激光共焦顯微鏡、免疫組織化學、原位雜交、同位素標記等各種儀器設備。質量標準血清質量高低取決于兩方面因素：一是取材對象，二是取材過程。用于取材的動物應健康無病并且在指定的出生天數之內，取材過程應嚴格按照操作規程執行，制備出的血清要經過嚴格的質量鑒定。1． 牛血清必須來自有文件證明無牛海綿狀腦病的牛群或國家。并應具備適當的監測系統。2． 有些國家還要求牛血清來自未用過反芻動物蛋白飼料的牛群。3． 證明所用牛血清中不含對所生產疫苗病毒的抑制物。4． 血清要通過濾膜過濾除菌，保證無菌。5． 無細菌、霉菌、支原體和病毒的污染，有些國家要求無細菌噬菌體污染。6． 對細胞有良好的支持繁殖作用
抗凝綿羊血（無菌 pet瓶裝） 注意事項: 盡管經測試Annexin V-FITC反復凍融5次對于其檢測效果無顯著影響,但為取得良好的使用效果,3-6個月內推薦4℃保存,并適當注意避免反復凍融。 如果有細菌或真菌污染,會嚴重影響檢測效果。 染色后宜盡快檢測,時間過長可能會導致凋亡或壞死細胞的數量增加。 如果細胞收集過程中使用了胰酶,需注意設法去除殘留的胰酶。殘留的胰酶會消化并降解Annexin V-FITC,導致染色失敗。 熒光物質均易發生淬滅,在進行熒光觀察時,盡量縮短觀察時間,同時在操作和存放過程中也盡量注意避光保存。 用于流式細胞儀檢測時,如果發現Annexin V-FITC單獨染色時出現了過多的PI假陽性細胞,并且通過調整相關設置和參數也無法改善,可以用PBS將Annexin V-FITC稀釋3-10倍后再進行檢測,這樣通常可以有效減少假陽性的壞死細胞。 需自備PBS。 本產品僅限于專業人員的科學研究用,不得用于臨床診斷或,不得用于食品或藥品,不得存放于普通住宅內。 為了您的安全和健康,請穿實驗服并戴一次性手套操作。操作步驟(僅供參考):1、貼壁細胞的消化①吸除培養液,用無菌 PBS、Hanks 液或無血清培養液洗滌細胞一次,以去除殘余的血清。②加入少量 Trypsin-EDTA solution,略蓋過細胞即可,室溫放置 0.5~2min, 不同的細胞消化時間有所不同。③顯微鏡下觀察,細胞明顯收縮,并且肉眼觀察培養器皿底部發現細胞的形態發生明顯的變化;或者用槍吹打細胞發現細胞剛好可以被吹打下來,吸除胰酶細胞消化液。加入含血清的 完全細胞培養液,吹打下細胞,即可直接用于后續實驗。④如果發現消化不足,則加入 Trypsin-EDTA solution 重新消化。⑤如果發現細胞消化時間過長,未及吹打細胞,細胞已經有部分直接從培養器皿底部脫落, 直接用胰酶細胞培養液把細胞全部吹打下來。1000~2000g 離心 1min,沉淀細胞,盡量去除胰酶細胞消化液后,加入含血清的完全培養液重新懸浮細胞,即可用于后續實驗。2、組織的消化不同的組織需要消化的時間相差很大,通常以消化后可以充分打散組織為宜。鑒別方法： 血液凝結析出的淡黃色通明液體。如將血液自血管內抽出，放入試管中，不加抗凝劑，則凝血反響被激活，血液敏捷凝結，構成膠凍。其周圍所析出之淡黃色通明液體即為血清，也可于凝血后經離心獲得。在凝血過程中，纖維蛋白原轉成為纖維蛋白塊，所以血清中無纖維蛋白原，這一點是與血漿zui大的差異。而在凝血反響中，血小板開釋出很多物質，各也都發生了改變。 這些成分都留在血清中并持續發生改變，如原成為，并隨血清寄存時刻逐步削減以致不見。這些也都是與血漿差異的地方。但很多未參與凝血反響的物質則與血漿根本一樣。為防止抗凝劑的攪擾，血液中很多化學成分的剖析，都以血清為樣品。血清保存應該注意以下幾點：(1)需要長期保存的血清必須儲存于-20℃-70℃低溫冰箱中.4℃冰箱中保存時間切勿超過1個月.由于血清結冰時體積會增加約10%,因此,血清在凍入低溫冰箱前,必須預留一定體積空間,否則易發生污染或玻璃瓶凍裂.(2)熱滅活是指56℃,30分鐘加熱已*解凍的血清.加熱過程中須規則搖晃均勻.此熱處理的目的是使血清中的補體成分(complement)滅活.除非必須,一般不建議作此熱處理,因為熱處理會造成血清沉淀物顯著增多,而且還會影響血清的質量.補體參與反應有:細胞毒作用,平滑肌細胞收縮,肥大細胞和血小板釋放組胺,增強吞噬作用,促進淋巴細胞和巨噬細胞發生化學趨化和活化.(3)瓶裝血清解凍需采用逐步解凍法:-20℃至-70℃低溫冰箱中的血清放入4℃冰箱中溶解1天.然后移入室溫,待全部溶解后再分裝.在溶解過程中需不斷輕輕搖晃均勻(小心勿造成氣泡),使溫度與成分均一,減少沉淀的發生.切勿直接將血清從-20℃進入37℃解凍,這樣因溫度改變太大,容易造成蛋白質凝集而出現沉淀.(4)一般廠商提供的血清為無菌,無需再過濾除菌.如發現血清有懸浮物,則可將血清加入培養液內一起過濾,切勿直接過濾血清.(5)血清中的沉淀物絮狀物:主要是血清中的脂蛋白變性及解凍后血清中纖維蛋白造成,這些絮狀物不會影響血清本身的質量.可用離心3000rpm,5分鐘去除,也可不用處理.顯微鏡下小黑點:經過熱處理過的血清,沉淀物的形成會顯著增多.有些沉淀物在顯微鏡下觀察象小黑點,常誤認為血清受污染.一般情況下,此小黑點不會影響細胞生長,但如果懷疑血清質量,則應立即停止使用,更換另一批號的血清.(6)切勿將血清在37℃放置太久,否則血清會變得渾濁,同時血清中的有效成分會破會而影響血清質量.抗凝綿羊血（無菌 pet瓶裝） 用法 血清由冰凍狀態在常溫下完全融化成液體,搖均勻后放置 10-30 分鐘待結塊蛋白質沉底后即可使用。注意事項 有少量蛋白質結塊析出不影響使用。未融化或融化未經搖勻的血清禁止直接放入高溫水浴內加溫。減少血清重復凍融。血清在室溫或 4 ℃ 冰箱內放置過久會影響促細胞生長效果。解凍 將血清從冰凍區中取出后(切勿直接將血清從 -20 ℃ 進入 37 ℃ 解凍,這樣因溫度改變太大,容易造成蛋白質凝集而出現沉淀),先置于 2 ~ 8 ℃ 區域中使之融化,然后在室溫下使之全融。使用前再預溫到將使用的溫度(如 37 ℃ ),但必須注意的是,解凍過程中應輕輕地搖晃均勻。血清在解凍過程中可能會出現少量絮狀或片狀析出物,主要是血清中脂蛋白的變性或是纖維蛋白(形成凝血的蛋白之一)析出,但這些析出物,并不影響血清本身的質量。 若一次無法用完一瓶,建議無菌分裝血清至恰當的滅菌容器內,再放回冷凍。細胞培養的優點:1.研究的對象是活細胞在實驗過程中,根據要求可始終保持細胞活力,并可長時間監控、檢測甚至定量評估一部分活細胞的情況,包括活細胞的形態、結構、生命活動等。2.研究條件可以人為控制pH、溫度、氧氣、二氧化碳、張力等物理化學的條件,可以根據實際需要進行人為的控制,同時,可以施加化學、物理、生物等因素作為條件而進行實驗觀察,這些因素同樣可以處于嚴格控制之下。3.研究的樣本可以達到比較均一性通過細胞培養一定代數后,所得到的細胞系則可以達到均一性而屬于同一類型的細胞,需要時,可采用克隆化等方法使細胞達到純化。4.研究內容便于觀察、檢測和記錄采用各種研究技術:如倒置生物顯微鏡、熒光顯微鏡、電子顯微鏡、流式細胞術、激光共焦顯微鏡、免疫組織化學、原位雜交、同位素標記等各種儀器設備。質量標準血清質量高低取決于兩方面因素：一是取材對象，二是取材過程。用于取材的動物應健康無病并且在指定的出生天數之內，取材過程應嚴格按照操作規程執行，制備出的血清要經過嚴格的質量鑒定。1． 牛血清必須來自有文件證明無牛海綿狀腦病的牛群或國家。并應具備適當的監測系統。2． 有些國家還要求牛血清來自未用過反芻動物蛋白飼料的牛群。3． 證明所用牛血清中不含對所生產疫苗病毒的抑制物。4． 血清要通過濾膜過濾除菌，保證無菌。5． 無細菌、霉菌、支原體和病毒的污染，有些國家要求無細菌噬菌體污染。6． 對細胞有良好的支持繁殖作用
抗凝山羊血（無菌 袋裝） 注意事項: 盡管經測試Annexin V-FITC反復凍融5次對于其檢測效果無顯著影響,但為取得良好的使用效果,3-6個月內推薦4℃保存,并適當注意避免反復凍融。 如果有細菌或真菌污染,會嚴重影響檢測效果。 染色后宜盡快檢測,時間過長可能會導致凋亡或壞死細胞的數量增加。 如果細胞收集過程中使用了胰酶,需注意設法去除殘留的胰酶。殘留的胰酶會消化并降解Annexin V-FITC,導致染色失敗。 熒光物質均易發生淬滅,在進行熒光觀察時,盡量縮短觀察時間,同時在操作和存放過程中也盡量注意避光保存。 用于流式細胞儀檢測時,如果發現Annexin V-FITC單獨染色時出現了過多的PI假陽性細胞,并且通過調整相關設置和參數也無法改善,可以用PBS將Annexin V-FITC稀釋3-10倍后再進行檢測,這樣通常可以有效減少假陽性的壞死細胞。 需自備PBS。 本產品僅限于專業人員的科學研究用,不得用于臨床診斷或,不得用于食品或藥品,不得存放于普通住宅內。 為了您的安全和健康,請穿實驗服并戴一次性手套操作。操作步驟(僅供參考):1、貼壁細胞的消化①吸除培養液,用無菌 PBS、Hanks 液或無血清培養液洗滌細胞一次,以去除殘余的血清。②加入少量 Trypsin-EDTA solution,略蓋過細胞即可,室溫放置 0.5~2min, 不同的細胞消化時間有所不同。③顯微鏡下觀察,細胞明顯收縮,并且肉眼觀察培養器皿底部發現細胞的形態發生明顯的變化;或者用槍吹打細胞發現細胞剛好可以被吹打下來,吸除胰酶細胞消化液。加入含血清的 完全細胞培養液,吹打下細胞,即可直接用于后續實驗。④如果發現消化不足,則加入 Trypsin-EDTA solution 重新消化。⑤如果發現細胞消化時間過長,未及吹打細胞,細胞已經有部分直接從培養器皿底部脫落, 直接用胰酶細胞培養液把細胞全部吹打下來。1000~2000g 離心 1min,沉淀細胞,盡量去除胰酶細胞消化液后,加入含血清的完全培養液重新懸浮細胞,即可用于后續實驗。2、組織的消化不同的組織需要消化的時間相差很大,通常以消化后可以充分打散組織為宜。鑒別方法： 血液凝結析出的淡黃色通明液體。如將血液自血管內抽出，放入試管中，不加抗凝劑，則凝血反響被激活，血液敏捷凝結，構成膠凍。其周圍所析出之淡黃色通明液體即為血清，也可于凝血后經離心獲得。在凝血過程中，纖維蛋白原轉成為纖維蛋白塊，所以血清中無纖維蛋白原，這一點是與血漿zui大的差異。而在凝血反響中，血小板開釋出很多物質，各也都發生了改變。 這些成分都留在血清中并持續發生改變，如原成為，并隨血清寄存時刻逐步削減以致不見。這些也都是與血漿差異的地方。但很多未參與凝血反響的物質則與血漿根本一樣。為防止抗凝劑的攪擾，血液中很多化學成分的剖析，都以血清為樣品。血清保存應該注意以下幾點：(1)需要長期保存的血清必須儲存于-20℃-70℃低溫冰箱中.4℃冰箱中保存時間切勿超過1個月.由于血清結冰時體積會增加約10%,因此,血清在凍入低溫冰箱前,必須預留一定體積空間,否則易發生污染或玻璃瓶凍裂.(2)熱滅活是指56℃,30分鐘加熱已*解凍的血清.加熱過程中須規則搖晃均勻.此熱處理的目的是使血清中的補體成分(complement)滅活.除非必須,一般不建議作此熱處理,因為熱處理會造成血清沉淀物顯著增多,而且還會影響血清的質量.補體參與反應有:細胞毒作用,平滑肌細胞收縮,肥大細胞和血小板釋放組胺,增強吞噬作用,促進淋巴細胞和巨噬細胞發生化學趨化和活化.(3)瓶裝血清解凍需采用逐步解凍法:-20℃至-70℃低溫冰箱中的血清放入4℃冰箱中溶解1天.然后移入室溫,待全部溶解后再分裝.在溶解過程中需不斷輕輕搖晃均勻(小心勿造成氣泡),使溫度與成分均一,減少沉淀的發生.切勿直接將血清從-20℃進入37℃解凍,這樣因溫度改變太大,容易造成蛋白質凝集而出現沉淀.(4)一般廠商提供的血清為無菌,無需再過濾除菌.如發現血清有懸浮物,則可將血清加入培養液內一起過濾,切勿直接過濾血清.(5)血清中的沉淀物絮狀物:主要是血清中的脂蛋白變性及解凍后血清中纖維蛋白造成,這些絮狀物不會影響血清本身的質量.可用離心3000rpm,5分鐘去除,也可不用處理.顯微鏡下小黑點:經過熱處理過的血清,沉淀物的形成會顯著增多.有些沉淀物在顯微鏡下觀察象小黑點,常誤認為血清受污染.一般情況下,此小黑點不會影響細胞生長,但如果懷疑血清質量,則應立即停止使用,更換另一批號的血清.(6)切勿將血清在37℃放置太久,否則血清會變得渾濁,同時血清中的有效成分會破會而影響血清質量.抗凝山羊血（無菌 袋裝） 用法 血清由冰凍狀態在常溫下完全融化成液體,搖均勻后放置 10-30 分鐘待結塊蛋白質沉底后即可使用。注意事項 有少量蛋白質結塊析出不影響使用。未融化或融化未經搖勻的血清禁止直接放入高溫水浴內加溫。減少血清重復凍融。血清在室溫或 4 ℃ 冰箱內放置過久會影響促細胞生長效果。解凍 將血清從冰凍區中取出后(切勿直接將血清從 -20 ℃ 進入 37 ℃ 解凍,這樣因溫度改變太大,容易造成蛋白質凝集而出現沉淀),先置于 2 ~ 8 ℃ 區域中使之融化,然后在室溫下使之全融。使用前再預溫到將使用的溫度(如 37 ℃ ),但必須注意的是,解凍過程中應輕輕地搖晃均勻。血清在解凍過程中可能會出現少量絮狀或片狀析出物,主要是血清中脂蛋白的變性或是纖維蛋白(形成凝血的蛋白之一)析出,但這些析出物,并不影響血清本身的質量。 若一次無法用完一瓶,建議無菌分裝血清至恰當的滅菌容器內,再放回冷凍。細胞培養的優點:1.研究的對象是活細胞在實驗過程中,根據要求可始終保持細胞活力,并可長時間監控、檢測甚至定量評估一部分活細胞的情況,包括活細胞的形態、結構、生命活動等。2.研究條件可以人為控制pH、溫度、氧氣、二氧化碳、張力等物理化學的條件,可以根據實際需要進行人為的控制,同時,可以施加化學、物理、生物等因素作為條件而進行實驗觀察,這些因素同樣可以處于嚴格控制之下。3.研究的樣本可以達到比較均一性通過細胞培養一定代數后,所得到的細胞系則可以達到均一性而屬于同一類型的細胞,需要時,可采用克隆化等方法使細胞達到純化。4.研究內容便于觀察、檢測和記錄采用各種研究技術:如倒置生物顯微鏡、熒光顯微鏡、電子顯微鏡、流式細胞術、激光共焦顯微鏡、免疫組織化學、原位雜交、同位素標記等各種儀器設備。質量標準血清質量高低取決于兩方面因素：一是取材對象，二是取材過程。用于取材的動物應健康無病并且在指定的出生天數之內，取材過程應嚴格按照操作規程執行，制備出的血清要經過嚴格的質量鑒定。1． 牛血清必須來自有文件證明無牛海綿狀腦病的牛群或國家。并應具備適當的監測系統。2． 有些國家還要求牛血清來自未用過反芻動物蛋白飼料的牛群。3． 證明所用牛血清中不含對所生產疫苗病毒的抑制物。4． 血清要通過濾膜過濾除菌，保證無菌。5． 無細菌、霉菌、支原體和病毒的污染，有些國家要求無細菌噬菌體污染。6． 對細胞有良好的支持繁殖作用
抗凝山羊血（無菌 pet瓶裝） 用法 血清由冰凍狀態在常溫下完全融化成液體,搖均勻后放置 10-30 分鐘待結塊蛋白質沉底后即可使用。注意事項 有少量蛋白質結塊析出不影響使用。未融化或融化未經搖勻的血清禁止直接放入高溫水浴內加溫。減少血清重復凍融。血清在室溫或 4 ℃ 冰箱內放置過久會影響促細胞生長效果。解凍 將血清從冰凍區中取出后(切勿直接將血清從 -20 ℃ 進入 37 ℃ 解凍,這樣因溫度改變太大,容易造成蛋白質凝集而出現沉淀),先置于 2 ~ 8 ℃ 區域中使之融化,然后在室溫下使之全融。使用前再預溫到將使用的溫度(如 37 ℃ ),但必須注意的是,解凍過程中應輕輕地搖晃均勻。血清在解凍過程中可能會出現少量絮狀或片狀析出物,主要是血清中脂蛋白的變性或是纖維蛋白(形成凝血的蛋白之一)析出,但這些析出物,并不影響血清本身的質量。 若一次無法用完一瓶,建議無菌分裝血清至恰當的滅菌容器內,再放回冷凍。細胞培養的優點:1.研究的對象是活細胞在實驗過程中,根據要求可始終保持細胞活力,并可長時間監控、檢測甚至定量評估一部分活細胞的情況,包括活細胞的形態、結構、生命活動等。2.研究條件可以人為控制pH、溫度、氧氣、二氧化碳、張力等物理化學的條件,可以根據實際需要進行人為的控制,同時,可以施加化學、物理、生物等因素作為條件而進行實驗觀察,這些因素同樣可以處于嚴格控制之下。3.研究的樣本可以達到比較均一性通過細胞培養一定代數后,所得到的細胞系則可以達到均一性而屬于同一類型的細胞,需要時,可采用克隆化等方法使細胞達到純化。4.研究內容便于觀察、檢測和記錄采用各種研究技術:如倒置生物顯微鏡、熒光顯微鏡、電子顯微鏡、流式細胞術、激光共焦顯微鏡、免疫組織化學、原位雜交、同位素標記等各種儀器設備。鑒別方法： 血液凝結析出的淡黃色通明液體。如將血液自血管內抽出，放入試管中，不加抗凝劑，則凝血反響被激活，血液敏捷凝結，構成膠凍。其周圍所析出之淡黃色通明液體即為血清，也可于凝血后經離心獲得。在凝血過程中，纖維蛋白原轉成為纖維蛋白塊，所以血清中無纖維蛋白原，這一點是與血漿zui大的差異。而在凝血反響中，血小板開釋出很多物質，各也都發生了改變。 這些成分都留在血清中并持續發生改變，如原成為，并隨血清寄存時刻逐步削減以致不見。這些也都是與血漿差異的地方。但很多未參與凝血反響的物質則與血漿根本一樣。為防止抗凝劑的攪擾，血液中很多化學成分的剖析，都以血清為樣品。血清保存應該注意以下幾點：(1)需要長期保存的血清必須儲存于-20℃-70℃低溫冰箱中.4℃冰箱中保存時間切勿超過1個月.由于血清結冰時體積會增加約10%,因此,血清在凍入低溫冰箱前,必須預留一定體積空間,否則易發生污染或玻璃瓶凍裂.(2)熱滅活是指56℃,30分鐘加熱已*解凍的血清.加熱過程中須規則搖晃均勻.此熱處理的目的是使血清中的補體成分(complement)滅活.除非必須,一般不建議作此熱處理,因為熱處理會造成血清沉淀物顯著增多,而且還會影響血清的質量.補體參與反應有:細胞毒作用,平滑肌細胞收縮,肥大細胞和血小板釋放組胺,增強吞噬作用,促進淋巴細胞和巨噬細胞發生化學趨化和活化.(3)瓶裝血清解凍需采用逐步解凍法:-20℃至-70℃低溫冰箱中的血清放入4℃冰箱中溶解1天.然后移入室溫,待全部溶解后再分裝.在溶解過程中需不斷輕輕搖晃均勻(小心勿造成氣泡),使溫度與成分均一,減少沉淀的發生.切勿直接將血清從-20℃進入37℃解凍,這樣因溫度改變太大,容易造成蛋白質凝集而出現沉淀.(4)一般廠商提供的血清為無菌,無需再過濾除菌.如發現血清有懸浮物,則可將血清加入培養液內一起過濾,切勿直接過濾血清.(5)血清中的沉淀物絮狀物:主要是血清中的脂蛋白變性及解凍后血清中纖維蛋白造成,這些絮狀物不會影響血清本身的質量.可用離心3000rpm,5分鐘去除,也可不用處理.顯微鏡下小黑點:經過熱處理過的血清,沉淀物的形成會顯著增多.有些沉淀物在顯微鏡下觀察象小黑點,常誤認為血清受污染.一般情況下,此小黑點不會影響細胞生長,但如果懷疑血清質量,則應立即停止使用,更換另一批號的血清.(6)切勿將血清在37℃放置太久,否則血清會變得渾濁,同時血清中的有效成分會破會而影響血清質量.抗凝山羊血（無菌 pet瓶裝） 注意事項: 盡管經測試Annexin V-FITC反復凍融5次對于其檢測效果無顯著影響,但為取得良好的使用效果,3-6個月內推薦4℃保存,并適當注意避免反復凍融。 如果有細菌或真菌污染,會嚴重影響檢測效果。 染色后宜盡快檢測,時間過長可能會導致凋亡或壞死細胞的數量增加。 如果細胞收集過程中使用了胰酶,需注意設法去除殘留的胰酶。殘留的胰酶會消化并降解Annexin V-FITC,導致染色失敗。 熒光物質均易發生淬滅,在進行熒光觀察時,盡量縮短觀察時間,同時在操作和存放過程中也盡量注意避光保存。 用于流式細胞儀檢測時,如果發現Annexin V-FITC單獨染色時出現了過多的PI假陽性細胞,并且通過調整相關設置和參數也無法改善,可以用PBS將Annexin V-FITC稀釋3-10倍后再進行檢測,這樣通常可以有效減少假陽性的壞死細胞。 需自備PBS。 本產品僅限于專業人員的科學研究用,不得用于臨床診斷或,不得用于食品或藥品,不得存放于普通住宅內。 為了您的安全和健康,請穿實驗服并戴一次性手套操作。操作步驟(僅供參考):1、貼壁細胞的消化①吸除培養液,用無菌 PBS、Hanks 液或無血清培養液洗滌細胞一次,以去除殘余的血清。②加入少量 Trypsin-EDTA solution,略蓋過細胞即可,室溫放置 0.5~2min, 不同的細胞消化時間有所不同。③顯微鏡下觀察,細胞明顯收縮,并且肉眼觀察培養器皿底部發現細胞的形態發生明顯的變化;或者用槍吹打細胞發現細胞剛好可以被吹打下來,吸除胰酶細胞消化液。加入含血清的 完全細胞培養液,吹打下細胞,即可直接用于后續實驗。④如果發現消化不足,則加入 Trypsin-EDTA solution 重新消化。⑤如果發現細胞消化時間過長,未及吹打細胞,細胞已經有部分直接從培養器皿底部脫落, 直接用胰酶細胞培養液把細胞全部吹打下來。1000~2000g 離心 1min,沉淀細胞,盡量去除胰酶細胞消化液后,加入含血清的完全培養液重新懸浮細胞,即可用于后續實驗。2、組織的消化不同的組織需要消化的時間相差很大,通常以消化后可以充分打散組織為宜。質量標準血清質量高低取決于兩方面因素：一是取材對象，二是取材過程。用于取材的動物應健康無病并且在指定的出生天數之內，取材過程應嚴格按照操作規程執行，制備出的血清要經過嚴格的質量鑒定。1． 牛血清必須來自有文件證明無牛海綿狀腦病的牛群或國家。并應具備適當的監測系統。2． 有些國家還要求牛血清來自未用過反芻動物蛋白飼料的牛群。3． 證明所用牛血清中不含對所生產疫苗病毒的抑制物。4． 血清要通過濾膜過濾除菌，保證無菌。5． 無細菌、霉菌、支原體和病毒的污染，有些國家要求無細菌噬菌體污染。6． 對細胞有良好的支持繁殖作用
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