標(biao)準雞(ji)(ji)(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（細(xi)(xi)(xi)(xi)菌(jun)(jun)培(pei)養(yang)(yang)(yang)(yang)專用(yong)(yong)(yong)(yong)(yong)(yong)） 適用(yong)(yong)(yong)(yong)(yong)(yong)范圍【產(chan)(chan)(chan)品(pin)(pin)(pin)名稱】 雞(ji)(ji)(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（無(wu)菌(jun)(jun)過(guo)(guo)濾(lv)）【產(chan)(chan)(chan)品(pin)(pin)(pin)規(gui)(gui)格】100ml/200ml/500ml/500ml*20瓶(ping)(ping)(ping)(ping)【產(chan)(chan)(chan)品(pin)(pin)(pin)價格】130元-4500元【適用(yong)(yong)(yong)(yong)(yong)(yong)范圍]適應(ying)于(yu)(yu)(yu)(yu)(yu)(yu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)(yang)、免(mian)疫(yi)(yi)檢測(ce)等(deng)(deng)(deng)(deng)。【儲存(cun)(cun)條件及(ji)(ji)(ji)有(you)(you)效期(qi)(qi)(qi)]1、密封保(bao)存(cun)(cun)于(yu)(yu)(yu)(yu)(yu)(yu)-10℃~-30℃2、有(you)(you)效期(qi)(qi)(qi)五年[注(zhu)意事項]1、本產(chan)(chan)(chan)品(pin)(pin)(pin)僅(jin)供(gong)科(ke)(ke)(ke)(ke)(ke)研(yan)使用(yong)(yong)(yong)(yong)(yong)(yong)。2、產(chan)(chan)(chan)品(pin)(pin)(pin)使用(yong)(yong)(yong)(yong)(yong)(yong)過(guo)(guo)程(cheng)中(zhong)，注(zhu)意防(fang)止污(wu)染(ran)(ran)，在(zai)(zai)室(shi)溫內(nei)放(fang)(fang)置過(guo)(guo)久會(hui)影響使用(yong)(yong)(yong)(yong)(yong)(yong)效果(guo)(guo)，使用(yong)(yong)(yong)(yong)(yong)(yong)后請盡快放(fang)(fang)入-10℃~-33、請勿將(jiang)(jiang)(jiang)使用(yong)(yong)(yong)(yong)(yong)(yong)后剩余(yu)(yu)的(de)(de)(de)(de)(de)(de)(de)試劑(ji)再(zai)倒入原(yuan)包裝瓶(ping)(ping)(ping)(ping)內(nei)。【生(sheng)(sheng)(sheng)產(chan)(chan)(chan)日期(qi)(qi)(qi)]詳(xiang)見標(biao)簽，請在(zai)(zai)產(chan)(chan)(chan)品(pin)(pin)(pin)標(biao)簽標(biao)注(zhu)的(de)(de)(de)(de)(de)(de)(de)有(you)(you)效期(qi)(qi)(qi)內(nei)使用(yong)(yong)(yong)(yong)(yong)(yong)。產(chan)(chan)(chan)品(pin)(pin)(pin)介(jie)紹 雞(ji)(ji)(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)(yang)中(zhong)的(de)(de)(de)(de)(de)(de)(de)天(tian)然培(pei)養(yang)(yang)(yang)(yang)基(ji)(ji)(ji),含有(you)(you)豐富(fu)的(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)長必(bi)(bi)須的(de)(de)(de)(de)(de)(de)(de)營(ying)養(yang)(yang)(yang)(yang)成(cheng)(cheng)(cheng)(cheng)份(fen),常用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)動物(wu)(wu)(wu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)體(ti)(ti)(ti)外(wai)培(pei)養(yang)(yang)(yang)(yang),具(ju)(ju)有(you)(you)極為重(zhong)要(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)功能(neng)(neng)。1.提(ti)供(gong)對維(wei)持(chi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)指(zhi)數生(sheng)(sheng)(sheng)長的(de)(de)(de)(de)(de)(de)(de)激(ji)(ji)素(su),基(ji)(ji)(ji)礎培(pei)養(yang)(yang)(yang)(yang)基(ji)(ji)(ji)中(zhong)沒(mei)有(you)(you)或量(liang)(liang)很少的(de)(de)(de)(de)(de)(de)(de)營(ying)養(yang)(yang)(yang)(yang)物(wu)(wu)(wu),以(yi)及(ji)(ji)(ji)主(zhu)要(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)低分(fen)子營(ying)養(yang)(yang)(yang)(yang)物(wu)(wu)(wu)。2. 提(ti)供(gong)結(jie)合(he)蛋(dan)白(bai)(bai)(bai)(bai),能(neng)(neng)識(shi)別(bie)維(wei)生(sheng)(sheng)(sheng)素(su)、脂類、金屬和(he)(he)其(qi)(qi)他(ta)激(ji)(ji)素(su)等(deng)(deng)(deng)(deng),能(neng)(neng)結(jie)合(he)或調變(bian)(bian)它(ta)們所結(jie)合(he)的(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)質(zhi)活力。3.有(you)(you)些情況下結(jie)合(he)蛋(dan)白(bai)(bai)(bai)(bai)質(zhi)能(neng)(neng)與(yu)有(you)(you)毒金屬和(he)(he)熱原(yuan)質(zhi)結(jie)合(he),起到作用(yong)(yong)(yong)(yong)(yong)(yong)。4.是(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)貼壁、鋪(pu)展在(zai)(zai)塑料培(pei)養(yang)(yang)(yang)(yang)基(ji)(ji)(ji)質(zhi)上(shang)所需因子來源。5.起酸堿(jian)度(du)緩沖液作用(yong)(yong)(yong)(yong)(yong)(yong)。 6.提(ti)供(gong)蛋(dan)白(bai)(bai)(bai)(bai)酶(mei)抑制劑(ji),使在(zai)(zai)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)傳(chuan)代時使剩余(yu)(yu)胰蛋(dan)白(bai)(bai)(bai)(bai)酶(mei)失活,保(bao)護細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)不(bu)(bu)受傷害(hai)。 雞(ji)(ji)(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)分(fen)和(he)(he)作用(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)研(yan)究(jiu)雖有(you)(you)很大進(jin)展,但仍存(cun)(cun)在(zai)(zai)一(yi)(yi)些問題。主(zhu)要(yao)(yao)是(shi):第(di)一(yi)(yi):血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)份(fen)可(ke)能(neng)(neng)有(you)(you)幾百種之(zhi)(zhi)多(duo)(duo),目前(qian)對其(qi)(qi)準確的(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)份(fen)、含量(liang)(liang)及(ji)(ji)(ji)其(qi)(qi)作用(yong)(yong)(yong)(yong)(yong)(yong)機制仍不(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚(chu),尤其(qi)(qi)是(shi)對其(qi)(qi)中(zhong)一(yi)(yi)些多(duo)(duo)肽類生(sheng)(sheng)(sheng)長因子、激(ji)(ji)素(su)和(he)(he)脂類等(deng)(deng)(deng)(deng)尚未(wei)充分(fen)認(ren)識(shi),這(zhe)給研(yan)究(jiu)工(gong)(gong)作帶來許多(duo)(duo)困難;第(di)二:血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都是(shi)批(pi)量(liang)(liang)生(sheng)(sheng)(sheng)產(chan)(chan)(chan),各批(pi)量(liang)(liang)之(zhi)(zhi)間(jian)差異(yi)(yi)很大,而(er)且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)保(bao)存(cun)(cun)期(qi)(qi)(qi)至(zhi)多(duo)(duo)一(yi)(yi)年,因此(ci),要(yao)(yao)保(bao)證每(mei)批(pi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)相似性極為困難,從(cong)而(er)使實驗的(de)(de)(de)(de)(de)(de)(de)標(biao)準化和(he)(he)連(lian)續性受到限制;第(di)三:不(bu)(bu)能(neng)(neng)排除(chu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)含有(you)(you)易變(bian)(bian)物(wu)(wu)(wu)質(zhi),這(zhe)被認(ren)為是(shi)“瓶(ping)(ping)(ping)(ping)中(zhong)”的(de)(de)(de)(de)(de)(de)(de)原(yuan)因之(zhi)(zhi)一(yi)(yi)。 雞(ji)(ji)(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)瓶(ping)(ping)(ping)(ping)裝血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)凍(dong)需采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)逐(zhu)步(bu)解(jie)(jie)(jie)(jie)凍(dong)法:-20℃ 至(zhi) -70℃ 低溫冰箱中(zhong)的(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)放(fang)(fang)入4℃冰箱中(zhong)溶(rong)解(jie)(jie)(jie)(jie)1天(tian).然后移入室(shi)溫,待全(quan)部溶(rong)解(jie)(jie)(jie)(jie)后再(zai)分(fen)裝.在(zai)(zai)溶(rong)解(jie)(jie)(jie)(jie)過(guo)(guo)程(cheng)中(zhong)需不(bu)(bu)斷(duan)輕輕搖(yao)晃(huang)均(jun)(jun)(jun)勻(小(xiao)心(xin)勿造成(cheng)(cheng)(cheng)(cheng)氣泡),使溫度(du)與(yu)成(cheng)(cheng)(cheng)(cheng)分(fen)均(jun)(jun)(jun)一(yi)(yi),減少沉淀的(de)(de)(de)(de)(de)(de)(de)發生(sheng)(sheng)(sheng).切勿直接(jie)將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)-20℃進(jin)入37℃解(jie)(jie)(jie)(jie)凍(dong),這(zhe)樣(yang)因溫度(du)改(gai)變(bian)(bian)太(tai)大,容易造成(cheng)(cheng)(cheng)(cheng)蛋(dan)白(bai)(bai)(bai)(bai)質(zhi)凝(ning)集而(er)出現沉淀.標(biao)準雞(ji)(ji)(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（細(xi)(xi)(xi)(xi)菌(jun)(jun)培(pei)養(yang)(yang)(yang)(yang)專用(yong)(yong)(yong)(yong)(yong)(yong)） 雞(ji)(ji)(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing) 的(de)(de)(de)(de)(de)(de)(de)其(qi)(qi)它(ta)產(chan)(chan)(chan)品(pin)(pin)(pin)特(te)點:雞(ji)(ji)(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)嬌貴細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)(yang)、細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)株(zhu)保(bao)存(cun)(cun)特(te)級(ji)新(xin)生(sheng)(sheng)(sheng)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)雜(za)交、骨(gu)質(zhi)、原(yuan)代、傳(chuan)代細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)等(deng)(deng)(deng)(deng)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)(yang)及(ji)(ji)(ji)疫(yi)(yi)苗(miao)、診(zhen)斷(duan)試劑(ji)生(sheng)(sheng)(sheng)產(chan)(chan)(chan)等(deng)(deng)(deng)(deng)馬血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)(ke)研(yan)、診(zhen)斷(duan)試劑(ji)生(sheng)(sheng)(sheng)產(chan)(chan)(chan)等(deng)(deng)(deng)(deng)山羊(yang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)(ke)研(yan)、診(zhen)斷(duan)試劑(ji)生(sheng)(sheng)(sheng)產(chan)(chan)(chan)等(deng)(deng)(deng)(deng)兔血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)(ke)研(yan)等(deng)(deng)(deng)(deng)驢血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)(ke)研(yan)、診(zhen)斷(duan)試劑(ji)生(sheng)(sheng)(sheng)產(chan)(chan)(chan)等(deng)(deng)(deng)(deng)豬血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)(ke)研(yan)、診(zhen)斷(duan)試劑(ji)生(sheng)(sheng)(sheng)產(chan)(chan)(chan)等(deng)(deng)(deng)(deng)雞(ji)(ji)(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)(ke)研(yan)、診(zhen)斷(duan)試劑(ji)生(sheng)(sheng)(sheng)產(chan)(chan)(chan)等(deng)(deng)(deng)(deng)無(wu)菌(jun)(jun)脫(tuo)纖維(wei)綿羊(yang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)(ke)研(yan)、診(zhen)斷(duan)試劑(ji)生(sheng)(sheng)(sheng)產(chan)(chan)(chan)等(deng)(deng)(deng)(deng)無(wu)菌(jun)(jun)脫(tuo)纖維(wei)兔血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)(ke)研(yan)、診(zhen)斷(duan)試劑(ji)生(sheng)(sheng)(sheng)產(chan)(chan)(chan)等(deng)(deng)(deng)(deng)羊(yang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)(ke)研(yan)等(deng)(deng)(deng)(deng)兔血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)(ke)研(yan)等(deng)(deng)(deng)(deng)產(chan)(chan)(chan)品(pin)(pin)(pin)應(ying)用(yong)(yong)(yong)(yong)(yong)(yong)： 用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)雜(za)交瘤細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)、骨(gu)質(zhi)瘤細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)、原(yuan)代、傳(chuan)代細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)等(deng)(deng)(deng)(deng)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)(yang)及(ji)(ji)(ji)疫(yi)(yi)苗(miao)、診(zhen)斷(duan)試劑(ji)生(sheng)(sheng)(sheng)產(chan)(chan)(chan)；雞(ji)(ji)(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)IgG的(de)(de)(de)(de)(de)(de)(de)純化及(ji)(ji)(ji)其(qi)(qi)單(dan)抗(kang)(kang)制備(bei)與(yu)鑒(jian)定(ding)： IgG是(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)主(zhu)要(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)免(mian)疫(yi)(yi)球蛋(dan)白(bai)(bai)(bai)(bai)(Ig),占血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)Ig總(zong)量(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)75%~80%,它(ta)是(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液中(zhong)主(zhu)要(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)抗(kang)(kang)體(ti)(ti)(ti)成(cheng)(cheng)(cheng)(cheng)分(fen),血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)IgG水平的(de)(de)(de)(de)(de)(de)(de)測(ce)定(ding),已是(shi)疫(yi)(yi)苗(miao)免(mian)疫(yi)(yi)效果(guo)(guo)評定(ding)和(he)(he)體(ti)(ti)(ti)液免(mian)疫(yi)(yi)研(yan)究(jiu)的(de)(de)(de)(de)(de)(de)(de)常用(yong)(yong)(yong)(yong)(yong)(yong)指(zhi)標(biao)。與(yu)常規(gui)(gui)免(mian)疫(yi)(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)相比(bi),單(dan)克隆(long)抗(kang)(kang)體(ti)(ti)(ti)具(ju)(ju)有(you)(you)與(yu)抗(kang)(kang)原(yuan)結(jie)合(he)的(de)(de)(de)(de)(de)(de)(de)高特(te)異(yi)(yi)性,是(shi)免(mian)疫(yi)(yi)學和(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)學研(yan)究(jiu)的(de)(de)(de)(de)(de)(de)(de)重(zhong)要(yao)(yao)工(gong)(gong)具(ju)(ju),在(zai)(zai)畜牧獸醫的(de)(de)(de)(de)(de)(de)(de)疾病診(zhen)斷(duan)與(yu)治療方(fang)面(mian)已經被廣泛應(ying)用(yong)(yong)(yong)(yong)(yong)(yong)。在(zai)(zai)養(yang)(yang)(yang)(yang)禽(qin)業中(zhong),運用(yong)(yong)(yong)(yong)(yong)(yong)這(zhe)些單(dan)克隆(long)抗(kang)(kang)體(ti)(ti)(ti)來檢測(ce)疫(yi)(yi)病特(te)異(yi)(yi)性的(de)(de)(de)(de)(de)(de)(de)抗(kang)(kang)體(ti)(ti)(ti),對全(quan)面(mian)、系統地了解(jie)(jie)(jie)(jie)禽(qin)的(de)(de)(de)(de)(de)(de)(de)機體(ti)(ti)(ti)對病原(yuan)體(ti)(ti)(ti)感染(ran)(ran)的(de)(de)(de)(de)(de)(de)(de)免(mian)疫(yi)(yi)應(ying)答反(fan)應(ying)并采(cai)取相應(ying)的(de)(de)(de)(de)(de)(de)(de)防(fang)制措施有(you)(you)重(zhong)要(yao)(yao)意義。雞(ji)(ji)(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)背景 雞(ji)(ji)(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)(yang)中(zhong)用(yong)(yong)(yong)(yong)(yong)(yong)量(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)天(tian)然培(pei)養(yang)(yang)(yang)(yang)基(ji)(ji)(ji)，含有(you)(you)豐富(fu)的(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)長必(bi)(bi)須的(de)(de)(de)(de)(de)(de)(de)營(ying)養(yang)(yang)(yang)(yang)成(cheng)(cheng)(cheng)(cheng)份(fen)，常用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)動物(wu)(wu)(wu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)體(ti)(ti)(ti)外(wai)培(pei)養(yang)(yang)(yang)(yang)，具(ju)(ju)有(you)(you)極為重(zhong)要(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)功能(neng)(neng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必(bi)(bi)須貯(zhu)存(cun)(cun)于(yu)(yu)(yu)(yu)(yu)(yu)–20～-70 oC，若(ruo)存(cun)(cun)放(fang)(fang)于(yu)(yu)(yu)(yu)(yu)(yu)4度(du)，不(bu)(bu)能(neng)(neng)超過(guo)(guo)一(yi)(yi)個月(yue)。如(ru)果(guo)(guo)一(yi)(yi)次無(wu)法用(yong)(yong)(yong)(yong)(yong)(yong)完一(yi)(yi)瓶(ping)(ping)(ping)(ping)，可(ke)將(jiang)(jiang)(jiang)40～45 ml分(fen)裝于(yu)(yu)(yu)(yu)(yu)(yu)無(wu)菌(jun)(jun)50 ml離心(xin)管(guan)(guan)中(zhong)，由(you)于(yu)(yu)(yu)(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結(jie)凍(dong)時體(ti)(ti)(ti)積(ji)會(hui)增加約10%，必(bi)(bi)須預(yu)留10%膨(peng)脹體(ti)(ti)(ti)積(ji)的(de)(de)(de)(de)(de)(de)(de)空間(jian)，否則易發生(sheng)(sheng)(sheng)污(wu)染(ran)(ran)或容器凍(dong)裂(lie)。 瓶(ping)(ping)(ping)(ping)裝(500ml)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)凍(dong)步(bu)驟(逐(zhu)步(bu)解(jie)(jie)(jie)(jie)凍(dong)法):從(cong)-20 oC或–70 oC取出放(fang)(fang)至(zhi)4 oC冰箱溶(rong)解(jie)(jie)(jie)(jie)一(yi)(yi)天(tian)，再(zai)置于(yu)(yu)(yu)(yu)(yu)(yu)室(shi)溫下全(quan)溶(rong)后分(fen)裝，一(yi)(yi)般50 ml無(wu)菌(jun)(jun)離心(xin)管(guan)(guan)可(ke)分(fen)裝40～45 ml。在(zai)(zai)溶(rong)解(jie)(jie)(jie)(jie)過(guo)(guo)程(cheng)中(zhong)須規(gui)(gui)則搖(yao)晃(huang)均(jun)(jun)(jun)勻(小(xiao)心(xin)勿造成(cheng)(cheng)(cheng)(cheng)氣泡)，使溫度(du)與(yu)成(cheng)(cheng)(cheng)(cheng)分(fen)均(jun)(jun)(jun)一(yi)(yi)，減少沈(shen)淀的(de)(de)(de)(de)(de)(de)(de)發生(sheng)(sheng)(sheng)。切勿直接(jie)由(you)–20 oC直接(jie)至(zhi)37 oC解(jie)(jie)(jie)(jie)凍(dong)，因溫度(du)改(gai)變(bian)(bian)太(tai)大，容易造成(cheng)(cheng)(cheng)(cheng)蛋(dan)白(bai)(bai)(bai)(bai)質(zhi)凝(ning)結(jie)而(er)發生(sheng)(sheng)(sheng)沈(shen)淀。勿將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)置于(yu)(yu)(yu)(yu)(yu)(yu)37 oC太(tai)久，若(ruo)在(zai)(zai)37 oC放(fang)(fang)置太(tai)久，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)會(hui)變(bian)(bian)得混濁，同時血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)許多(duo)(duo)不(bu)(bu)穩(wen)定(ding)的(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)份(fen)也會(hui)因此(ci)受到破壞，從(cong)而(er)影響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)品(pin)(pin)(pin)質(zhi)。
標(biao)準豬(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)培(pei)養(yang)(yang)(yang)(yang)(yang)專(zhuan)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)）規格:500ml/瓶保(bao)(bao)質(zhi)(zhi)(zhi)(zhi)(zhi)期(qi)(qi)(qi): 五(wu)年如(ru)(ru)(ru)何解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)才不(bu)(bu)(bu)(bu)(bu)會(hui)(hui)使(shi)(shi)(shi)(shi)產(chan)品(pin)(pin)(pin)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)受(shou)損? 將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)冷(leng)凍(dong)(dong)(dong)(dong)(dong)箱(xiang)(xiang)(xiang)(xiang)取出(chu)(chu)后(hou)(hou)(hou),先置于(yu)(yu)2-8℃冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)使(shi)(shi)(shi)(shi)之(zhi)(zhi)溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie),然后(hou)(hou)(hou)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)室(shi)溫(wen)下(xia)(xia)使(shi)(shi)(shi)(shi)之(zhi)(zhi)全溶(rong)(rong)(rong)(rong)。但(dan)必(bi)(bi)(bi)(bi)須(xu)(xu)注(zhu)意的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi),溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)過(guo)(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)必(bi)(bi)(bi)(bi)須(xu)(xu)規則(ze)(ze)(ze)地(di)搖(yao)(yao)晃(huang)均(jun)(jun)勻(yun)(yun)(yun)。1.實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)時(shi)(shi)(shi)(shi)根(gen)據(ju)試(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情況和(he)(he)(he)(he)(he)性(xing)(xing)(xing)質(zhi)(zhi)(zhi)(zhi)(zhi)進(jin)(jin)行必(bi)(bi)(bi)(bi)要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)防(fang)(fang)(fang)護(hu)(hu)。根(gen)據(ju)試(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)可能發生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)危險(xian)事故(gu)佩(pei)戴(dai)必(bi)(bi)(bi)(bi)要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)防(fang)(fang)(fang)護(hu)(hu)工具(ju),例如(ru)(ru)(ru)穿好試(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)服,戴(dai)橡膠手(shou)套,防(fang)(fang)(fang)護(hu)(hu)面具(ju),防(fang)(fang)(fang)毒(du)面具(ju)等(deng)(deng)(deng)。實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)前(qian)(qian),要(yao)(yao)(yao)(yao)注(zhu)意清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)理(li)試(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)場周(zhou)圍的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)安(an)(an)全隱(yin)患。檢(jian)查試(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)裝(zhuang)置、藥(yao)品(pin)(pin)(pin)和(he)(he)(he)(he)(he)相關物(wu)(wu)(wu)(wu)(wu)品(pin)(pin)(pin)是(shi)(shi)(shi)否有(you)不(bu)(bu)(bu)(bu)(bu)符合(he)要(yao)(yao)(yao)(yao)求的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情況等(deng)(deng)(deng)。 2.遵循化(hua)學(xue)(xue)藥(yao)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)(xing)質(zhi)(zhi)(zhi)(zhi)(zhi)和(he)(he)(he)(he)(he)化(hua)學(xue)(xue)反(fan)(fan)應的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)規律,不(bu)(bu)(bu)(bu)(bu)盲目(mu)蠻(man)干(gan)和(he)(he)(he)(he)(he)主觀臆測(ce)化(hua)學(xue)(xue)反(fan)(fan)應的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)(guo)程(cheng)。應根(gen)據(ju)化(hua)學(xue)(xue)反(fan)(fan)應的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)(xing)質(zhi)(zhi)(zhi)(zhi)(zhi)和(he)(he)(he)(he)(he)過(guo)(guo)(guo)(guo)(guo)程(cheng)選擇(ze)匹配(pei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)反(fan)(fan)應裝(zhuang)置,不(bu)(bu)(bu)(bu)(bu)可圖省(sheng)事省(sheng)去(qu)必(bi)(bi)(bi)(bi)要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)安(an)(an)全措施。 實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)事故(gu)雖不(bu)(bu)(bu)(bu)(bu)可預(yu)測(ce),但(dan)其(qi)危險(xian)性(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)大(da)小(xiao)是(shi)(shi)(shi)可以(yi)(yi)估計到(dao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。保(bao)(bao)存(cun)方法:(1)長(chang)(chang)(chang)期(qi)(qi)(qi)保(bao)(bao)存(cun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必(bi)(bi)(bi)(bi)須(xu)(xu)儲存(cun)于(yu)(yu)-20℃ - 70℃ 低(di)溫(wen)冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)。4℃冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)存(cun)時(shi)(shi)(shi)(shi)間(jian)(jian)(jian)(jian)(jian)切(qie)(qie)(qie)(qie)勿(wu)(wu)(wu)超(chao)過(guo)(guo)(guo)(guo)(guo)1個(ge)月。由(you)于(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結(jie)(jie)冰(bing)(bing)時(shi)(shi)(shi)(shi)體積(ji)(ji)會(hui)(hui)增(zeng)加(jia)(jia)(jia)約(yue)10%,因(yin)(yin)(yin)此,血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)凍(dong)(dong)(dong)(dong)(dong)入(ru)低(di)溫(wen)冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)前(qian)(qian),必(bi)(bi)(bi)(bi)須(xu)(xu)預(yu)留(liu)一(yi)(yi)(yi)(yi)(yi)(yi)定(ding)體積(ji)(ji)空間(jian)(jian)(jian)(jian)(jian),否則(ze)(ze)(ze)易(yi)發生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)污染或(huo)玻璃(li)瓶凍(dong)(dong)(dong)(dong)(dong)裂。 (2)一(yi)(yi)(yi)(yi)(yi)(yi)般 提(ti)供(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun),無(wu)(wu)(wu)(wu)(wu)需(xu)(xu)(xu)(xu)再過(guo)(guo)(guo)(guo)(guo)濾(lv)除(chu)菌(jun)(jun)(jun)(jun)。如(ru)(ru)(ru)發現血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)有(you)懸浮物(wu)(wu)(wu)(wu)(wu),則(ze)(ze)(ze)可將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)加(jia)(jia)(jia)入(ru)培(pei)養(yang)(yang)(yang)(yang)(yang)液內一(yi)(yi)(yi)(yi)(yi)(yi)起(qi)(qi)過(guo)(guo)(guo)(guo)(guo)濾(lv),切(qie)(qie)(qie)(qie)勿(wu)(wu)(wu)直(zhi)接(jie)(jie)過(guo)(guo)(guo)(guo)(guo)濾(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。(3)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong): 瓶裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)需(xu)(xu)(xu)(xu)采用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)逐步解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)法:-20℃ 至(zhi)(zhi)(zhi) -70℃ 低(di)溫(wen)冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)放(fang)(fang)入(ru)4℃冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)1天。然后(hou)(hou)(hou)移(yi)(yi)入(ru)室(shi)溫(wen),待全部溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)后(hou)(hou)(hou)再分(fen)(fen)(fen)(fen)(fen)裝(zhuang)。在(zai)(zai)(zai)(zai)(zai)(zai)(zai)溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)過(guo)(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)需(xu)(xu)(xu)(xu)不(bu)(bu)(bu)(bu)(bu)斷輕(qing)輕(qing)搖(yao)(yao)晃(huang)均(jun)(jun)勻(yun)(yun)(yun)(小(xiao)心勿(wu)(wu)(wu)造(zao)成(cheng)(cheng)(cheng)(cheng)(cheng)氣泡(pao)),使(shi)(shi)(shi)(shi)溫(wen)度(du)與成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)均(jun)(jun)一(yi)(yi)(yi)(yi)(yi)(yi),減(jian)少(shao)沉淀(dian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)。切(qie)(qie)(qie)(qie)勿(wu)(wu)(wu)直(zhi)接(jie)(jie)將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)-20℃進(jin)(jin)入(ru)37℃解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong),這樣因(yin)(yin)(yin)溫(wen)度(du)改(gai)變太(tai)大(da),容(rong)易(yi)造(zao)成(cheng)(cheng)(cheng)(cheng)(cheng)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)凝集(ji)(ji)而出(chu)(chu)現沉淀(dian)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)留(liu)應當(dang)(dang)留(liu)意以(yi)(yi)下(xia)(xia)幾點(dian):1需(xu)(xu)(xu)(xu)求長(chang)(chang)(chang)時(shi)(shi)(shi)(shi)間(jian)(jian)(jian)(jian)(jian)保(bao)(bao)存(cun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)有(you)必(bi)(bi)(bi)(bi)要(yao)(yao)(yao)(yao)儲存(cun)于(yu)(yu)-20℃ - 70℃ 低(di)溫(wen)冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong).4℃冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)存(cun)時(shi)(shi)(shi)(shi)間(jian)(jian)(jian)(jian)(jian)切(qie)(qie)(qie)(qie)勿(wu)(wu)(wu)超(chao)越(yue)1個(ge)月.因(yin)(yin)(yin)為(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結(jie)(jie)冰(bing)(bing)時(shi)(shi)(shi)(shi)體積(ji)(ji)會(hui)(hui)添(tian)(tian)加(jia)(jia)(jia)約(yue)10%,因(yin)(yin)(yin)此,血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)凍(dong)(dong)(dong)(dong)(dong)入(ru)低(di)溫(wen)冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)前(qian)(qian),有(you)必(bi)(bi)(bi)(bi)要(yao)(yao)(yao)(yao)預(yu)留(liu)必(bi)(bi)(bi)(bi)定(ding)體積(ji)(ji)空間(jian)(jian)(jian)(jian)(jian),否則(ze)(ze)(ze)易(yi)發生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)污染或(huo)玻璃(li)瓶凍(dong)(dong)(dong)(dong)(dong)裂.2.熱(re)滅(mie)活(huo)(huo)(huo)是(shi)(shi)(shi)指56℃, 30分(fen)(fen)(fen)(fen)(fen)鐘加(jia)(jia)(jia)熱(re)已(yi)完(wan)全凍(dong)(dong)(dong)(dong)(dong)住的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).加(jia)(jia)(jia)熱(re)過(guo)(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)須(xu)(xu)規矩(ju)搖(yao)(yao)晃(huang)均(jun)(jun)勻(yun)(yun)(yun).此熱(re)處(chu)理(li)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)目(mu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)使(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補(bu)體成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(complement)滅(mie)活(huo)(huo)(huo).除(chu)非(fei)有(you)必(bi)(bi)(bi)(bi)要(yao)(yao)(yao)(yao),一(yi)(yi)(yi)(yi)(yi)(yi)般不(bu)(bu)(bu)(bu)(bu)建(jian)議作(zuo)(zuo)此熱(re)處(chu)理(li),因(yin)(yin)(yin)為(wei)熱(re)處(chu)理(li)會(hui)(hui)構(gou)(gou)成(cheng)(cheng)(cheng)(cheng)(cheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)堆(dui)(dui)積(ji)(ji)物(wu)(wu)(wu)(wu)(wu)明(ming)(ming)顯(xian)(xian)增(zeng)多(duo),而且還會(hui)(hui)影(ying)(ying)(ying)(ying)響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang).補(bu)體參(can)與反(fan)(fan)響(xiang)有(you):細(xi)(xi)(xi)胞(bao)(bao)(bao)毒(du)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong), 滑潤肌細(xi)(xi)(xi)胞(bao)(bao)(bao)縮短, 肥大(da)細(xi)(xi)(xi)胞(bao)(bao)(bao)和(he)(he)(he)(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)板開(kai)釋組(zu)胺(an), 增(zeng)強(qiang)吞(tun)噬作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong), 推動和(he)(he)(he)(he)(he)發生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)學(xue)(xue)趨化(hua)和(he)(he)(he)(he)(he)活(huo)(huo)(huo)化(hua).3.瓶裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)凍(dong)(dong)(dong)(dong)(dong)住需(xu)(xu)(xu)(xu)選用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)逐步凍(dong)(dong)(dong)(dong)(dong)住法:-20℃ 至(zhi)(zhi)(zhi) -70℃ 低(di)溫(wen)冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)放(fang)(fang)入(ru)4℃冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)1天.然后(hou)(hou)(hou)移(yi)(yi)入(ru)室(shi)溫(wen),待全部溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)后(hou)(hou)(hou)再分(fen)(fen)(fen)(fen)(fen)裝(zhuang).在(zai)(zai)(zai)(zai)(zai)(zai)(zai)溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)過(guo)(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)需(xu)(xu)(xu)(xu)不(bu)(bu)(bu)(bu)(bu)斷悄悄搖(yao)(yao)晃(huang)均(jun)(jun)勻(yun)(yun)(yun)(當(dang)(dang)心勿(wu)(wu)(wu)構(gou)(gou)成(cheng)(cheng)(cheng)(cheng)(cheng)氣泡(pao)),使(shi)(shi)(shi)(shi)溫(wen)度(du)與成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)均(jun)(jun)一(yi)(yi)(yi)(yi)(yi)(yi),削減(jian)堆(dui)(dui)積(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng).切(qie)(qie)(qie)(qie)勿(wu)(wu)(wu)直(zhi)接(jie)(jie)將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)-20℃進(jin)(jin)入(ru)37℃凍(dong)(dong)(dong)(dong)(dong)住,這么因(yin)(yin)(yin)溫(wen)度(du)改(gai)動太(tai)大(da),簡略(lve)構(gou)(gou)成(cheng)(cheng)(cheng)(cheng)(cheng)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)凝集(ji)(ji)而出(chu)(chu)現堆(dui)(dui)積(ji)(ji).4.一(yi)(yi)(yi)(yi)(yi)(yi)般 供(gong)應的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun),無(wu)(wu)(wu)(wu)(wu)需(xu)(xu)(xu)(xu)再過(guo)(guo)(guo)(guo)(guo)濾(lv)除(chu)菌(jun)(jun)(jun)(jun).如(ru)(ru)(ru)發現血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)有(you)懸浮物(wu)(wu)(wu)(wu)(wu),則(ze)(ze)(ze)可將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)參(can)加(jia)(jia)(jia)培(pei)養(yang)(yang)(yang)(yang)(yang)液內一(yi)(yi)(yi)(yi)(yi)(yi)同過(guo)(guo)(guo)(guo)(guo)濾(lv),切(qie)(qie)(qie)(qie)勿(wu)(wu)(wu)直(zhi)接(jie)(jie)過(guo)(guo)(guo)(guo)(guo)濾(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).5.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)堆(dui)(dui)積(ji)(ji)物(wu)(wu)(wu)(wu)(wu) 絮(xu)(xu)狀物(wu)(wu)(wu)(wu)(wu):主要(yao)(yao)(yao)(yao)是(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)脂蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)變性(xing)(xing)(xing)及凍(dong)(dong)(dong)(dong)(dong)住后(hou)(hou)(hou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)纖(xian)(xian)維(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)構(gou)(gou)成(cheng)(cheng)(cheng)(cheng)(cheng),這些(xie)(xie)絮(xu)(xu)狀物(wu)(wu)(wu)(wu)(wu)不(bu)(bu)(bu)(bu)(bu)會(hui)(hui)影(ying)(ying)(ying)(ying)響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本(ben)身的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang).可用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)離心3000rpm, 5分(fen)(fen)(fen)(fen)(fen)鐘去(qu)掉(diao),也可不(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)處(chu)理(li). 顯(xian)(xian)微鏡下(xia)(xia)"小(xiao)黑點(dian)":通過(guo)(guo)(guo)(guo)(guo)熱(re)處(chu)理(li)過(guo)(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),堆(dui)(dui)積(ji)(ji)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)構(gou)(gou)成(cheng)(cheng)(cheng)(cheng)(cheng)會(hui)(hui)明(ming)(ming)顯(xian)(xian)增(zeng)多(duo).有(you)些(xie)(xie)堆(dui)(dui)積(ji)(ji)物(wu)(wu)(wu)(wu)(wu)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)顯(xian)(xian)微鏡下(xia)(xia)查詢象(xiang)"小(xiao)黑點(dian)",常(chang)(chang)(chang)誤(wu)認(ren)為(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)受(shou)污染.一(yi)(yi)(yi)(yi)(yi)(yi)般情況下(xia)(xia),此小(xiao)黑點(dian)不(bu)(bu)(bu)(bu)(bu)會(hui)(hui)影(ying)(ying)(ying)(ying)響(xiang)細(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang),但(dan)假(jia)(jia)(jia)如(ru)(ru)(ru)懷疑(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang),則(ze)(ze)(ze)應當(dang)(dang)即(ji)停止(zhi)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong),替換另(ling)一(yi)(yi)(yi)(yi)(yi)(yi)批號的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).6.切(qie)(qie)(qie)(qie)勿(wu)(wu)(wu)將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)37℃放(fang)(fang)置太(tai)久,否則(ze)(ze)(ze)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)會(hui)(hui)變得渾濁,一(yi)(yi)(yi)(yi)(yi)(yi)同血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)效(xiao)成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)會(hui)(hui)破會(hui)(hui)而影(ying)(ying)(ying)(ying)響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)標(biao)準豬(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)培(pei)養(yang)(yang)(yang)(yang)(yang)專(zhuan)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)）背景豬(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)系采用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)成(cheng)(cheng)(cheng)(cheng)(cheng)年健康豬(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液為(wei)原(yuan)(yuan)(yuan)(yuan)料加(jia)(jia)(jia)工而成(cheng)(cheng)(cheng)(cheng)(cheng)，經無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)采集(ji)(ji)、分(fen)(fen)(fen)(fen)(fen)離、微孔過(guo)(guo)(guo)(guo)(guo)濾(lv)分(fen)(fen)(fen)(fen)(fen)裝(zhuang)而成(cheng)(cheng)(cheng)(cheng)(cheng)。無(wu)(wu)(wu)(wu)(wu)支原(yuan)(yuan)(yuan)(yuan)體、無(wu)(wu)(wu)(wu)(wu)噬菌(jun)(jun)(jun)(jun)體，內毒(du)素(su)含(han)(han)量(liang)(liang)低(di)于(yu)(yu)50EU/ml。豬(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)過(guo)(guo)(guo)(guo)(guo)濾(lv)）在(zai)(zai)(zai)(zai)(zai)(zai)(zai)細(xi)(xi)(xi)胞(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)：1.提(ti)供(gong)基本(ben)營養(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)：氨基酸(suan)、維(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素(su)、無(wu)(wu)(wu)(wu)(wu)機物(wu)(wu)(wu)(wu)(wu)、脂類物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)、核酸(suan)衍生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)等(deng)(deng)(deng)，是(shi)(shi)(shi)細(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)必(bi)(bi)(bi)(bi)須(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)。2.提(ti)供(gong)激(ji)素(su)和(he)(he)(he)(he)(he)各種生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)子(zi)：胰(yi)島素(su)、腎上腺皮(pi)質(zhi)(zhi)(zhi)(zhi)(zhi)激(ji)素(su)（地(di)塞(sai)米松）、類固醇(chun)激(ji)素(su)（雌二(er)醇(chun)、睪酮、孕酮）等(deng)(deng)(deng)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)子(zi)如(ru)(ru)(ru)成(cheng)(cheng)(cheng)(cheng)(cheng)纖(xian)(xian)維(wei)(wei)細(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)子(zi)、表皮(pi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)子(zi)、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)板生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)(yin)子(zi)等(deng)(deng)(deng)。3.提(ti)供(gong)結(jie)(jie)合(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)：結(jie)(jie)合(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)攜帶重要(yao)(yao)(yao)(yao)地(di)低(di)分(fen)(fen)(fen)(fen)(fen)子(zi)量(liang)(liang)物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)，如(ru)(ru)(ru)白(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)攜帶維(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素(su)、脂肪、以(yi)(yi)及激(ji)素(su)等(deng)(deng)(deng)，轉鐵蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)攜帶鐵。結(jie)(jie)合(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)細(xi)(xi)(xi)胞(bao)(bao)(bao)代謝(xie)過(guo)(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)起(qi)(qi)重要(yao)(yao)(yao)(yao)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。提(ti)供(gong)促接(jie)(jie)觸和(he)(he)(he)(he)(he)伸(shen)展因(yin)(yin)(yin)子(zi)使(shi)(shi)(shi)(shi)細(xi)(xi)(xi)胞(bao)(bao)(bao)貼壁免受(shou)機械(xie)損傷。4.對培(pei)養(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)胞(bao)(bao)(bao)起(qi)(qi)到(dao)某(mou)些(xie)(xie)保(bao)(bao)護(hu)(hu)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)：有(you)一(yi)(yi)(yi)(yi)(yi)(yi)些(xie)(xie)細(xi)(xi)(xi)胞(bao)(bao)(bao)，如(ru)(ru)(ru)內皮(pi)細(xi)(xi)(xi)胞(bao)(bao)(bao)、骨髓樣細(xi)(xi)(xi)胞(bao)(bao)(bao)可以(yi)(yi)釋放(fang)(fang)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)酶，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)含(han)(han)有(you)抗(kang)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)酶成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)，起(qi)(qi)到(dao)中(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。這種作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)偶然發現的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，現在(zai)(zai)(zai)(zai)(zai)(zai)(zai)則(ze)(ze)(ze)有(you)目(mu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)來終(zhong)止(zhi)胰(yi)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)酶的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消化(hua)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。因(yin)(yin)(yin)為(wei)胰(yi)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)酶已(yi)經被廣泛用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)貼壁細(xi)(xi)(xi)胞(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消化(hua)傳(chuan)代。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)形成(cheng)(cheng)(cheng)(cheng)(cheng)了血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)粘(zhan)度(du)，可以(yi)(yi)保(bao)(bao)護(hu)(hu)細(xi)(xi)(xi)胞(bao)(bao)(bao)免受(shou)機械(xie)損傷，特(te)別(bie)是(shi)(shi)(shi)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)懸浮培(pei)養(yang)(yang)(yang)(yang)(yang)攪拌時(shi)(shi)(shi)(shi)，粘(zhan)度(du)起(qi)(qi)到(dao)重要(yao)(yao)(yao)(yao)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)還含(han)(han)有(you)一(yi)(yi)(yi)(yi)(yi)(yi)些(xie)(xie)微量(liang)(liang)元素(su)和(he)(he)(he)(he)(he)離子(zi)，他(ta)們在(zai)(zai)(zai)(zai)(zai)(zai)(zai)代謝(xie)解(jie)(jie)(jie)(jie)(jie)毒(du)中(zhong)(zhong)(zhong)(zhong)(zhong)起(qi)(qi)重要(yao)(yao)(yao)(yao)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)(ru)SeO3,硒等(deng)(deng)(deng)。使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)注(zhu)意事項1.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)采用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)逐步解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)法（-20℃→2—8℃→25℃或(huo)37℃），解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)過(guo)(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)必(bi)(bi)(bi)(bi)須(xu)(xu)隨時(shi)(shi)(shi)(shi)輕(qing)搖(yao)(yao)，使(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)受(shou)熱(re)溫(wen)度(du)均(jun)(jun)勻(yun)(yun)(yun)。2. 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)凝絮(xu)(xu)物(wu)(wu)(wu)(wu)(wu)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)后(hou)(hou)(hou)會(hui)(hui)出(chu)(chu)現少(shao)量(liang)(liang)片狀或(huo)絮(xu)(xu)狀物(wu)(wu)(wu)(wu)(wu)析出(chu)(chu)，這主要(yao)(yao)(yao)(yao)是(shi)(shi)(shi)由(you)于(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)內不(bu)(bu)(bu)(bu)(bu)穩定(ding)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)變性(xing)(xing)(xing)、纖(xian)(xian)維(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)析出(chu)(chu)形成(cheng)(cheng)(cheng)(cheng)(cheng)沉淀(dian)物(wu)(wu)(wu)(wu)(wu)，實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)證明(ming)(ming)沉淀(dian)物(wu)(wu)(wu)(wu)(wu)不(bu)(bu)(bu)(bu)(bu)會(hui)(hui)影(ying)(ying)(ying)(ying)響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本(ben)身質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)。;3. 熱(re)滅(mie)活(huo)(huo)(huo)本(ben)產(chan)品(pin)(pin)(pin)除(chu)注(zhu)明(ming)(ming)外均(jun)(jun)未(wei)滅(mie)活(huo)(huo)(huo)。如(ru)(ru)(ru)果必(bi)(bi)(bi)(bi)須(xu)(xu)滅(mie)活(huo)(huo)(huo)，請(qing)嚴格按(an)照56℃30分(fen)(fen)(fen)(fen)(fen)鐘（水浴）處(chu)理(li)已(yi)解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。4. 常(chang)(chang)(chang)溫(wen)狀態下(xia)(xia)短期(qi)(qi)(qi)融化(hua)并(bing)不(bu)(bu)(bu)(bu)(bu)會(hui)(hui)影(ying)(ying)(ying)(ying)響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)。5.產(chan)品(pin)(pin)(pin)有(you)效(xiao)期(qi)(qi)(qi)，檢(jian)定(ding)合(he)格之(zhi)(zhi)日(ri)起(qi)(qi)有(you)效(xiao)期(qi)(qi)(qi)5年。解(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)：將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)冷(leng)凍(dong)(dong)(dong)(dong)(dong)箱(xiang)(xiang)(xiang)(xiang)取出(chu)(chu)后(hou)(hou)(hou)，先置于(yu)(yu)2-8℃冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)使(shi)(shi)(shi)(shi)之(zhi)(zhi)溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)，然后(hou)(hou)(hou)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)室(shi)溫(wen)下(xia)(xia)使(shi)(shi)(shi)(shi)之(zhi)(zhi)全溶(rong)(rong)(rong)(rong)。但(dan)必(bi)(bi)(bi)(bi)須(xu)(xu)注(zhu)意的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)，溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)過(guo)(guo)(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)必(bi)(bi)(bi)(bi)須(xu)(xu)規則(ze)(ze)(ze)地(di)搖(yao)(yao)晃(huang)均(jun)(jun)勻(yun)(yun)(yun)。二(er)、保(bao)(bao)存(cun)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)應保(bao)(bao)存(cun)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)-5℃至(zhi)(zhi)(zhi)-2O℃若(ruo)存(cun)放(fang)(fang)于(yu)(yu)4℃時(shi)(shi)(shi)(shi)，請(qing)勿(wu)(wu)(wu)超(chao)過(guo)(guo)(guo)(guo)(guo)一(yi)(yi)(yi)(yi)(yi)(yi)個(ge)月。若(ruo)一(yi)(yi)(yi)(yi)(yi)(yi)次無(wu)(wu)(wu)(wu)(wu)法用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)一(yi)(yi)(yi)(yi)(yi)(yi)瓶，建(jian)議將(jiang)(jiang)無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)分(fen)(fen)(fen)(fen)(fen)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)至(zhi)(zhi)(zhi)恰(qia)當(dang)(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)滅(mie)菌(jun)(jun)(jun)(jun)容(rong)器(qi)內，再放(fang)(fang)回冷(leng)凍(dong)(dong)(dong)(dong)(dong)。豬(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)過(guo)(guo)(guo)(guo)(guo)濾(lv)）注(zhu)意事項：（1）有(you)少(shao)量(liang)(liang)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)結(jie)(jie)塊析出(chu)(chu)不(bu)(bu)(bu)(bu)(bu)影(ying)(ying)(ying)(ying)響(xiang)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。未(wei)融化(hua)或(huo)融化(hua)未(wei)經搖(yao)(yao)勻(yun)(yun)(yun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)禁(jin)止(zhi)直(zhi)接(jie)(jie)放(fang)(fang)入(ru)高溫(wen)水浴內加(jia)(jia)(jia)溫(wen)。減(jian)少(shao)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)重復凍(dong)(dong)(dong)(dong)(dong)融。若(ruo)您(nin)一(yi)(yi)(yi)(yi)(yi)(yi)次無(wu)(wu)(wu)(wu)(wu)法用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)一(yi)(yi)(yi)(yi)(yi)(yi)瓶，建(jian)議您(nin)無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)分(fen)(fen)(fen)(fen)(fen)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)至(zhi)(zhi)(zhi)恰(qia)當(dang)(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)滅(mie)菌(jun)(jun)(jun)(jun)容(rong)器(qi)內，再放(fang)(fang)回冷(leng)凍(dong)(dong)(dong)(dong)(dong)。（2）長(chang)(chang)(chang)期(qi)(qi)(qi)保(bao)(bao)存(cun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必(bi)(bi)(bi)(bi)須(xu)(xu)儲存(cun)于(yu)(yu)-20℃-70℃低(di)溫(wen)冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)。4℃冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)存(cun)時(shi)(shi)(shi)(shi)間(jian)(jian)(jian)(jian)(jian)切(qie)(qie)(qie)(qie)勿(wu)(wu)(wu)超(chao)過(guo)(guo)(guo)(guo)(guo)1個(ge)月。由(you)于(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結(jie)(jie)冰(bing)(bing)時(shi)(shi)(shi)(shi)體積(ji)(ji)會(hui)(hui)增(zeng)加(jia)(jia)(jia)約(yue)10%,因(yin)(yin)(yin)此，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)凍(dong)(dong)(dong)(dong)(dong)入(ru)低(di)溫(wen)冰(bing)(bing)箱(xiang)(xiang)(xiang)(xiang)前(qian)(qian)，必(bi)(bi)(bi)(bi)須(xu)(xu)預(yu)留(liu)一(yi)(yi)(yi)(yi)(yi)(yi)定(ding)體積(ji)(ji)空間(jian)(jian)(jian)(jian)(jian)，否則(ze)(ze)(ze)易(yi)發生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)污染或(huo)玻璃(li)瓶凍(dong)(dong)(dong)(dong)(dong)裂。（3）一(yi)(yi)(yi)(yi)(yi)(yi)般廠商提(ti)供(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)無(wu)(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)，無(wu)(wu)(wu)(wu)(wu)需(xu)(xu)(xu)(xu)再過(guo)(guo)(guo)(guo)(guo)濾(lv)除(chu)菌(jun)(jun)(jun)(jun)。如(ru)(ru)(ru)發現血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)有(you)懸浮物(wu)(wu)(wu)(wu)(wu)，則(ze)(ze)(ze)可將(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)加(jia)(jia)(jia)入(ru)培(pei)養(yang)(yang)(yang)(yang)(yang)液內一(yi)(yi)(yi)(yi)(yi)(yi)起(qi)(qi)過(guo)(guo)(guo)(guo)(guo)濾(lv)，切(qie)(qie)(qie)(qie)勿(wu)(wu)(wu)直(zhi)接(jie)(jie)過(guo)(guo)(guo)(guo)(guo)濾(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。介(jie)紹(shao) 疫(yi)(yi)(yi)苗(miao)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)預(yu)防(fang)(fang)(fang)豬(zhu)(zhu)(zhu)(zhu)(zhu)常(chang)(chang)(chang)見(jian)傳(chuan)染病(bing)方面起(qi)(qi)著非(fei)常(chang)(chang)(chang)重要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。 它包括豬(zhu)(zhu)(zhu)(zhu)(zhu)瘟(wen)疫(yi)(yi)(yi)苗(miao)、豬(zhu)(zhu)(zhu)(zhu)(zhu)肺炎支原(yuan)(yuan)(yuan)(yuan)體疫(yi)(yi)(yi)苗(miao)以(yi)(yi)及正(zheng)處(chu)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)研(yan)發熱(re)點(dian)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)非(fei)洲豬(zhu)(zhu)(zhu)(zhu)(zhu)瘟(wen)等(deng)(deng)(deng)疫(yi)(yi)(yi)苗(miao)。 在(zai)(zai)(zai)(zai)(zai)(zai)(zai)豬(zhu)(zhu)(zhu)(zhu)(zhu)病(bing)毒(du)疫(yi)(yi)(yi)苗(miao)和(he)(he)(he)(he)(he)肺炎支原(yuan)(yuan)(yuan)(yuan)體疫(yi)(yi)(yi)苗(miao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)中(zhong)(zhong)(zhong)(zhong)(zhong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)常(chang)(chang)(chang)見(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)細(xi)(xi)(xi)胞(bao)(bao)(bao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)養(yang)(yang)(yang)(yang)(yang)液成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)。 尤其(qi)是(shi)(shi)(shi)豬(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)豬(zhu)(zhu)(zhu)(zhu)(zhu)肺炎支原(yuan)(yuan)(yuan)(yuan)體疫(yi)(yi)(yi)苗(miao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)。在(zai)(zai)(zai)(zai)(zai)(zai)(zai)對細(xi)(xi)(xi)胞(bao)(bao)(bao)和(he)(he)(he)(he)(he)活(huo)(huo)(huo)疫(yi)(yi)(yi)苗(miao)進(jin)(jin)行支原(yuan)(yuan)(yuan)(yuan)體檢(jian)驗(yan)(yan)(yan)(yan)(yan)(yan)中(zhong)(zhong)(zhong)(zhong)(zhong)，《獸藥(yao)典》也明(ming)(ming)確推薦了豬(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)作(zuo)(zuo)為(wei)支原(yuan)(yuan)(yuan)(yuan)體檢(jian)驗(yan)(yan)(yan)(yan)(yan)(yan)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)液體培(pei)養(yang)(yang)(yang)(yang)(yang)基的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)。并(bing)且，《獸藥(yao)典》指出(chu)(chu)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)【豬(zhu)(zhu)(zhu)(zhu)(zhu)支原(yuan)(yuan)(yuan)(yuan)體肺炎微量(liang)(liang)間(jian)(jian)(jian)(jian)(jian)接(jie)(jie)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)凝試(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)】和(he)(he)(he)(he)(he)【豬(zhu)(zhu)(zhu)(zhu)(zhu)支氣管敗血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)波(bo)氏桿菌(jun)(jun)(jun)(jun)凝集(ji)(ji)試(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)(yan)】中(zhong)(zhong)(zhong)(zhong)(zhong)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)陰性(xing)(xing)(xing)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，即(ji)正(zheng)常(chang)(chang)(chang)豬(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。 在(zai)(zai)(zai)(zai)(zai)(zai)(zai)豬(zhu)(zhu)(zhu)(zhu)(zhu)瘟(wen)疫(yi)(yi)(yi)苗(miao)等(deng)(deng)(deng)其(qi)他(ta)豬(zhu)(zhu)(zhu)(zhu)(zhu)病(bing)毒(du)疫(yi)(yi)(yi)苗(miao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)中(zhong)(zhong)(zhong)(zhong)(zhong)，常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)豬(zhu)(zhu)(zhu)(zhu)(zhu)腎細(xi)(xi)(xi)胞(bao)(bao)(bao)（PK-15）或(huo)豬(zhu)(zhu)(zhu)(zhu)(zhu)睪丸細(xi)(xi)(xi)胞(bao)(bao)(bao)（ST）進(jin)(jin)行病(bing)毒(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)繁殖。其(qi)培(pei)養(yang)(yang)(yang)(yang)(yang)液除(chu)添(tian)(tian)加(jia)(jia)(jia)MEM外，常(chang)(chang)(chang)添(tian)(tian)加(jia)(jia)(jia)新生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。但(dan)從(cong)品(pin)(pin)(pin)系相同的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)角度(du)來看，豬(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)更為(wei)合(he)適。 實(shi)(shi)(shi)際上早在(zai)(zai)(zai)(zai)(zai)(zai)(zai)1989年，國外就(jiu)建(jian)議用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)豬(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)取代牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)培(pei)養(yang)(yang)(yang)(yang)(yang)制造(zao)非(fei)洲豬(zhu)(zhu)(zhu)(zhu)(zhu)瘟(wen)病(bing)毒(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)胞(bao)(bao)(bao)（見(jian)《動物(wu)(wu)(wu)(wu)(wu)檢(jian)疫(yi)(yi)(yi)》 1991年01期(qi)(qi)(qi)《豬(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)白(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)抗(kang)體引起(qi)(qi)非(fei)洲豬(zhu)(zhu)(zhu)(zhu)(zhu)瘟(wen)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)學(xue)(xue)診斷的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)假(jia)(jia)(jia)陽性(xing)(xing)(xing)反(fan)(fan)應》），原(yuan)(yuan)(yuan)(yuan)因(yin)(yin)(yin)是(shi)(shi)(shi)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)白(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)會(hui)(hui)干(gan)擾非(fei)洲豬(zhu)(zhu)(zhu)(zhu)(zhu)瘟(wen)抗(kang)體的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)測(ce)，造(zao)成(cheng)(cheng)(cheng)(cheng)(cheng)假(jia)(jia)(jia)陽性(xing)(xing)(xing)反(fan)(fan)應。
標(biao)準綿(mian)(mian)羊血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)（細(xi)(xi)菌培養(yang)(yang)專(zhuan)用(yong)(yong)）產(chan)(chan)(chan)品(pin)描(miao)述綿(mian)(mian)羊血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)采集自(zi)正(zheng)常(chang)健(jian)康的(de)(de)(de)(de)(de)(de)(de)綿(mian)(mian)羊供(gong)(gong)體，經脂質(zhi)萃取和透析(xi)后(hou)儲存于(yu)(yu)(yu)緩(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)（0.01 M磷酸(suan)鈉(na)，0.25 M氯化鈉(na), pH 7.6）中(zhong)(zhong)。常(chang)用(yong)(yong)于(yu)(yu)(yu)各(ge)種免疫(yi)實驗，起到(dao)封閉或對照的(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)。本(ben)(ben)品(pin)用(yong)(yong)于(yu)(yu)(yu)封閉的(de)(de)(de)(de)(de)(de)(de)常(chang)見使(shi)(shi)用(yong)(yong)濃(nong)度為(wei)5%（v/v）。產(chan)(chan)(chan)品(pin)使(shi)(shi)用(yong)(yong)1）綿(mian)(mian)羊血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)以(yi)(yi)凍(dong)(dong)(dong)干粉形(xing)(xing)式(shi)提(ti)供(gong)(gong)的(de)(de)(de)(de)(de)(de)(de)，本(ben)(ben)品(pin)需(xu)要(yao)先(xian)溶(rong)解(jie)(jie)(jie)配制成(cheng)100%原(yuan)(yuan)液(ye)(ye)(ye)(ye)(ye)后(hou)再稀(xi)釋(shi)使(shi)(shi)用(yong)(yong)。溶(rong)解(jie)(jie)(jie)方(fang)法(fa)：用(yong)(yong)10ml 蒸餾水(shui)（dH2O）充分(fen)溶(rong)解(jie)(jie)(jie)凍(dong)(dong)(dong)干粉（如(ru)果不(bu)(bu)澄清(qing)(qing)(qing)(qing)(qing)(qing)，可(ke)對其(qi)離心處理），此(ci)(ci)時(shi)(shi)得(de)(de)到(dao)的(de)(de)(de)(de)(de)(de)(de)即是(shi)(shi)100%原(yuan)(yuan)液(ye)(ye)(ye)(ye)(ye)。然后(hou)按照1:20的(de)(de)(de)(de)(de)(de)(de)比例(li)用(yong)(yong)緩(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)，如(ru)PBS或PBST，稀(xi)釋(shi)到(dao)需(xu)要(yao)的(de)(de)(de)(de)(de)(de)(de)5%工作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)。也(ye)可(ke)以(yi)(yi)根據自(zi)身實驗條(tiao)件，稀(xi)釋(shi)到(dao)合適(shi)的(de)(de)(de)(de)(de)(de)(de)工作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)濃(nong)度。2）綿(mian)(mian)羊血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)以(yi)(yi)100%原(yuan)(yuan)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)形(xing)(xing)式(shi)提(ti)供(gong)(gong)，直接用(yong)(yong)合適(shi)的(de)(de)(de)(de)(de)(de)(de)緩(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)稀(xi)釋(shi)到(dao)需(xu)要(yao)的(de)(de)(de)(de)(de)(de)(de)工作(zuo)(zuo)(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)。產(chan)(chan)(chan)品(pin)性質(zhi)蛋(dan)白濃(nong)度（Protein concentration）60 mg/mL緩(huan)沖(chong)液(ye)(ye)(ye)(ye)(ye)（Buffer）0.01 M磷酸(suan)鈉(na)，0.25 M氯化鈉(na), pH 7.6防腐劑（Preservative）0.05%疊氮化鈉(na)運(yun)輸(shu)與(yu)(yu)保存方(fang)法(fa)冰(bing)(bing)袋運(yun)輸(shu)。凍(dong)(dong)(dong)干粉保存于(yu)(yu)(yu)2～8℃。100%原(yuan)(yuan)液(ye)(ye)(ye)(ye)(ye)保存于(yu)(yu)(yu)2～8℃，6個(ge)星(xing)期(qi)穩定。建議原(yuan)(yuan)液(ye)(ye)(ye)(ye)(ye)分(fen)裝后(hou)置于(yu)(yu)(yu)≤-20℃，以(yi)(yi)延(yan)長保存期(qi)。重溶(rong)后(hou)100%原(yuan)(yuan)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)有(you)(you)效期(qi)1年。注(zhu)意(yi)(yi)(yi)事項(xiang)1）本(ben)(ben)品(pin)含疊氮化鈉(na)，對人體有(you)(you)害，請(qing)(qing)注(zhu)意(yi)(yi)(yi)適(shi)當防護(hu)。2）為(wei)了(le)您的(de)(de)(de)(de)(de)(de)(de)安(an)全和健(jian)康，請(qing)(qing)穿實驗服(fu)并(bing)戴一(yi)(yi)(yi)次性手套操作(zuo)(zuo)(zuo)(zuo)。3）注(zhu)意(yi)(yi)(yi)無(wu)(wu)菌操作(zuo)(zuo)(zuo)(zuo)4）注(zhu)意(yi)(yi)(yi)避免反復凍(dong)(dong)(dong)融。反復凍(dong)(dong)(dong)融可(ke)能(neng)(neng)(neng)產(chan)(chan)(chan)生(sheng)(sheng)沉(chen)(chen)淀(dian)(dian)(dian)，可(ke)以(yi)(yi)通(tong)過(guo)無(wu)(wu)菌條(tiao)件下，3500rpm離心10min除(chu)去沉(chen)(chen)淀(dian)(dian)(dian)標(biao)準綿(mian)(mian)羊血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)（細(xi)(xi)菌培養(yang)(yang)專(zhuan)用(yong)(yong)）常(chang)見問題 保存方(fang)法(fa)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)應(ying)(ying)保存在-5℃至-20℃。然而(er)，若(ruo)(ruo)(ruo)存放(fang)于(yu)(yu)(yu)4℃時(shi)(shi)，請(qing)(qing)勿超過(guo)一(yi)(yi)(yi)個(ge)月。若(ruo)(ruo)(ruo)您一(yi)(yi)(yi)次無(wu)(wu)法(fa)用(yong)(yong)完一(yi)(yi)(yi)瓶(ping)，建議將(jiang)無(wu)(wu)菌分(fen)裝血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)至恰當的(de)(de)(de)(de)(de)(de)(de)滅(mie)菌容(rong)器(qi)內(nei)，再放(fang)回冷凍(dong)(dong)(dong)。解(jie)(jie)(jie)凍(dong)(dong)(dong)方(fang)法(fa)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)從冷凍(dong)(dong)(dong)箱取出(chu)(chu)(chu)后(hou)，先(xian)置于(yu)(yu)(yu)2-8℃冰(bing)(bing)箱使(shi)(shi)之(zhi)溶(rong)解(jie)(jie)(jie)，然后(hou)在室(shi)溫下使(shi)(shi)之(zhi)全溶(rong)。但必(bi)(bi)須注(zhu)意(yi)(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)，溶(rong)解(jie)(jie)(jie)過(guo)程(cheng)中(zhong)(zhong)必(bi)(bi)須規則(ze)(ze)地搖(yao)(yao)晃均(jun)(jun)勻(yun)。避免產(chan)(chan)(chan)生(sheng)(sheng)沉(chen)(chen)淀(dian)(dian)(dian)物(wu)1、解(jie)(jie)(jie)凍(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)，請(qing)(qing)按照所(suo)建議的(de)(de)(de)(de)(de)(de)(de)逐步解(jie)(jie)(jie)凍(dong)(dong)(dong)法(fa)（-2℃至4℃至室(shi)溫），若(ruo)(ruo)(ruo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)凍(dong)(dong)(dong)時(shi)(shi)改變(bian)的(de)(de)(de)(de)(de)(de)(de)溫度太(tai)大(da)（如(ru)-20℃至37℃），實驗顯示(shi)非(fei)(fei)(fei)常(chang)容(rong)易產(chan)(chan)(chan)生(sheng)(sheng)沉(chen)(chen)淀(dian)(dian)(dian)物(wu)。2、解(jie)(jie)(jie)凍(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)，請(qing)(qing)隨時(shi)(shi)將(jiang)之(zhi)搖(yao)(yao)晃均(jun)(jun)勻(yun)，使(shi)(shi)溫度及成(cheng)分(fen)均(jun)(jun)一(yi)(yi)(yi)，減(jian)少(shao)沉(chen)(chen)淀(dian)(dian)(dian)的(de)(de)(de)(de)(de)(de)(de)發(fa)(fa)生(sheng)(sheng)。3、請(qing)(qing)勿將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)置于(yu)(yu)(yu)37℃太(tai)久(jiu)(jiu)。若(ruo)(ruo)(ruo)在37℃放(fang)置太(tai)久(jiu)(jiu)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)會變(bian)得(de)(de)混濁(zhuo)，同時(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)許多較不(bu)(bu)穩定的(de)(de)(de)(de)(de)(de)(de)成(cheng)分(fen)也(ye)會因此(ci)(ci)受到(dao)損害，而(er)影(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)質(zhi)量。4、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)熱(re)滅(mie)活(huo)(huo)(huo)非(fei)(fei)(fei)常(chang)容(rong)易造(zao)成(cheng)沉(chen)(chen)淀(dian)(dian)(dian)物(wu)的(de)(de)(de)(de)(de)(de)(de)增(zeng)多，若(ruo)(ruo)(ruo)非(fei)(fei)(fei)必(bi)(bi)要(yao)，可(ke)以(yi)(yi)無(wu)(wu)須做(zuo)此(ci)(ci)步驟。 5、若(ruo)(ruo)(ruo)必(bi)(bi)須做(zuo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)熱(re)滅(mie)活(huo)(huo)(huo)，請(qing)(qing)遵守(shou)56℃，30分(fen)鐘的(de)(de)(de)(de)(de)(de)(de)原(yuan)(yuan)則(ze)(ze)，并(bing)且隨時(shi)(shi)搖(yao)(yao)晃均(jun)(jun)勻(yun)。溫度過(guo)高，時(shi)(shi)間(jian)過(guo)久(jiu)(jiu)或搖(yao)(yao)晃不(bu)(bu)均(jun)(jun)勻(yun)，都(dou)會造(zao)成(cheng)沉(chen)(chen)淀(dian)(dian)(dian)物(wu)的(de)(de)(de)(de)(de)(de)(de)增(zeng)多。若(ruo)(ruo)(ruo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)凍(dong)(dong)(dong)后(hou)發(fa)(fa)現有(you)(you)絮狀沉(chen)(chen)淀(dian)(dian)(dian)物(wu)出(chu)(chu)(chu)現，可(ke)以(yi)(yi)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)分(fen)裝至無(wu)(wu)菌離心管(guan)(guan)內(nei)，以(yi)(yi)400g稍(shao)微(wei)離心，上(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)即可(ke)接著加入(ru)培養(yang)(yang)基(ji)內(nei)一(yi)(yi)(yi)起過(guo)濾(lv)(lv)。但不(bu)(bu)建議以(yi)(yi)過(guo)濾(lv)(lv)的(de)(de)(de)(de)(de)(de)(de)方(fang)法(fa)去除(chu)這些絮狀沉(chen)(chen)淀(dian)(dian)(dian)物(wu)，因為(wei)它可(ke)能(neng)(neng)(neng)會阻塞過(guo)濾(lv)(lv)膜。熱(re)滅(mie)活(huo)(huo)(huo)一(yi)(yi)(yi)般(ban)是(shi)(shi)以(yi)(yi)56℃，30分(fen)鐘來處理已(yi)解(jie)(jie)(jie)凍(dong)(dong)(dong)的(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)，因為(wei)此(ci)(ci)加熱(re)步驟，可(ke)以(yi)(yi)使(shi)(shi)補(bu)體去活(huo)(huo)(huo)化，而(er)補(bu)體所(suo)參(can)與(yu)(yu)的(de)(de)(de)(de)(de)(de)(de)反應(ying)(ying)有(you)(you)：溶(rong)細(xi)(xi)胞(bao)(bao)(bao)活(huo)(huo)(huo)性，平滑(hua)肌(ji)的(de)(de)(de)(de)(de)(de)(de)收縮(suo)，肥大(da)細(xi)(xi)胞(bao)(bao)(bao)和血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小板(ban)組(zu)胺(an)的(de)(de)(de)(de)(de)(de)(de)釋(shi)放(fang)，增(zeng)強吞(tun)噬作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)，淋巴細(xi)(xi)胞(bao)(bao)(bao)、巨噬細(xi)(xi)胞(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)趨化和激(ji)(ji)活(huo)(huo)(huo)。鑒別(bie)方(fang)法(fa) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)凝(ning)(ning)固(gu)(gu)析(xi)出(chu)(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)淡黃色透明液(ye)(ye)(ye)(ye)(ye)體 。如(ru)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)自(zi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管(guan)(guan)內(nei)抽出(chu)(chu)(chu)，放(fang)入(ru)試管(guan)(guan)中(zhong)(zhong)，不(bu)(bu)加抗凝(ning)(ning)劑，則(ze)(ze)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反應(ying)(ying)被激(ji)(ji)活(huo)(huo)(huo)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)迅速凝(ning)(ning)固(gu)(gu)，形(xing)(xing)成(cheng)膠凍(dong)(dong)(dong)。凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)塊收縮(suo)，其(qi)周圍所(suo)析(xi)出(chu)(chu)(chu)之(zhi)淡黃色透明液(ye)(ye)(ye)(ye)(ye)體即為(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)，也(ye)可(ke)于(yu)(yu)(yu)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后(hou)經離心取得(de)(de)。在凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)過(guo)程(cheng)中(zhong)(zhong)，纖維(wei)蛋(dan)白原(yuan)(yuan)轉(zhuan)變(bian)成(cheng)纖維(wei)蛋(dan)白塊，所(suo)以(yi)(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)無(wu)(wu)纖維(wei)蛋(dan)白原(yuan)(yuan)，這一(yi)(yi)(yi)點是(shi)(shi)與(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿＊大(da)的(de)(de)(de)(de)(de)(de)(de)區(qu)別(bie)。而(er)在凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反應(ying)(ying)中(zhong)(zhong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小板(ban)釋(shi)放(fang)出(chu)(chu)(chu)許多物(wu)質(zhi)，各(ge)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)因子也(ye)都(dou)發(fa)(fa)生(sheng)(sheng)了(le)變(bian)化。這些成(cheng)分(fen)都(dou)留在血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)并(bing)繼續(xu)發(fa)(fa)生(sheng)(sheng)變(bian)化，如(ru)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)酶原(yuan)(yuan)變(bian)成(cheng)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)酶，并(bing)隨血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)存放(fang)時(shi)(shi)間(jian)逐漸減(jian)少(shao)以(yi)(yi)至消失。這些也(ye)都(dou)是(shi)(shi)與(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿區(qu)別(bie)之(zhi)處。但大(da)量未(wei)參(can)加凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)物(wu)質(zhi)則(ze)(ze)與(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿基(ji)本(ben)(ben)相(xiang)同。為(wei)避免抗凝(ning)(ning)劑的(de)(de)(de)(de)(de)(de)(de)干擾(rao)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)中(zhong)(zhong)許多化學成(cheng)分(fen)的(de)(de)(de)(de)(de)(de)(de)分(fen)析(xi)，都(dou)以(yi)(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)為(wei)樣(yang)品(pin)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)體成(cheng)分(fen)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)胞(bao)(bao)(bao)懸濁(zhuo)于(yu)(yu)(yu)其(qi)中(zhong)(zhong)。人體含有(you)(you)2750-3300毫升血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿，約占血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)總體積的(de)(de)(de)(de)(de)(de)(de)55%。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿的(de)(de)(de)(de)(de)(de)(de)絕(jue)大(da)部分(fen)是(shi)(shi)水(shui)（體積的(de)(de)(de)(de)(de)(de)(de)90%），其(qi)中(zhong)(zhong)溶(rong)解(jie)(jie)(jie)的(de)(de)(de)(de)(de)(de)(de)物(wu)質(zhi)主(zhu)(zhu)要(yao)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿蛋(dan)白，還包括葡萄糖(tang)、無(wu)(wu)機鹽離子、激(ji)(ji)素以(yi)(yi)及二氧化碳。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿的(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)要(yao)功能(neng)(neng)(neng)是(shi)(shi)運(yun)載血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)胞(bao)(bao)(bao)，同時(shi)(shi)也(ye)是(shi)(shi)運(yun)輸(shu)分(fen)泌產(chan)(chan)(chan)物(wu)的(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)要(yao)媒介。將(jiang)新鮮血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)離心，使(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)胞(bao)(bao)(bao)沉(chen)(chen)降，上(shang)層淡黃色清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)即是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿與(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)區(qu)別(bie)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)不(bu)(bu)含纖維(wei)蛋(dan)白原(yuan)(yuan)等(deng)凝(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)因子。
標(biao)準(zhun)山(shan)(shan)羊(yang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)（細(xi)(xi)菌(jun)(jun)(jun)培養(yang)(yang)專用(yong)(yong)）產(chan)(chan)品(pin)(pin)描述(shu)山(shan)(shan)羊(yang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)采集自正常(chang)健(jian)康(kang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)山(shan)(shan)羊(yang)供(gong)體(ti)，經(jing)脂(zhi)質萃取和(he)(he)透析后(hou)(hou)(hou)儲(chu)存(cun)(cun)于緩(huan)沖(chong)液(ye)(ye)（0.01 M磷酸(suan)(suan)鈉(na)，0.25 M氯(lv)化(hua)(hua)鈉(na), pH 7.6）中(zhong)(zhong)(zhong)。常(chang)用(yong)(yong)于各種免(mian)疫實(shi)驗，起(qi)到(dao)(dao)(dao)封(feng)閉或(huo)對照(zhao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)用(yong)(yong)。本(ben)品(pin)(pin)用(yong)(yong)于封(feng)閉的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)常(chang)見使(shi)(shi)(shi)用(yong)(yong)濃(nong)度(du)為(wei)5%（v/v）。產(chan)(chan)品(pin)(pin)使(shi)(shi)(shi)用(yong)(yong)1）山(shan)(shan)羊(yang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)是(shi)(shi)以(yi)(yi)凍(dong)干(gan)粉形(xing)式提供(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，本(ben)品(pin)(pin)需(xu)要(yao)先(xian)溶(rong)(rong)(rong)解(jie)配制(zhi)成100%原(yuan)液(ye)(ye)后(hou)(hou)(hou)再稀(xi)釋(shi)使(shi)(shi)(shi)用(yong)(yong)。溶(rong)(rong)(rong)解(jie)方法(fa)(fa)：用(yong)(yong)10ml 蒸餾水(shui)（dH2O）充分(fen)溶(rong)(rong)(rong)解(jie)凍(dong)干(gan)粉（如(ru)果(guo)不(bu)澄清(qing)(qing)，可(ke)(ke)對其(qi)離(li)(li)心(xin)處(chu)理），此(ci)(ci)時(shi)得(de)到(dao)(dao)(dao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)即是(shi)(shi)100%原(yuan)液(ye)(ye)。然(ran)后(hou)(hou)(hou)按(an)照(zhao)1:20的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比例用(yong)(yong)緩(huan)沖(chong)液(ye)(ye)，如(ru)PBS或(huo)PBST，稀(xi)釋(shi)到(dao)(dao)(dao)需(xu)要(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)5%工(gong)作(zuo)液(ye)(ye)。也(ye)可(ke)(ke)以(yi)(yi)根據自身實(shi)驗條件，稀(xi)釋(shi)到(dao)(dao)(dao)合適的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)工(gong)作(zuo)液(ye)(ye)濃(nong)度(du)。2）山(shan)(shan)羊(yang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)以(yi)(yi)100%原(yuan)液(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)形(xing)式提供(gong)，直(zhi)接用(yong)(yong)合適的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)緩(huan)沖(chong)液(ye)(ye)稀(xi)釋(shi)到(dao)(dao)(dao)需(xu)要(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)工(gong)作(zuo)液(ye)(ye)。產(chan)(chan)品(pin)(pin)性(xing)質蛋(dan)(dan)白(bai)(bai)濃(nong)度(du)（Protein concentration）60 mg/mL緩(huan)沖(chong)液(ye)(ye)（Buffer）0.01 M磷酸(suan)(suan)鈉(na)，0.25 M氯(lv)化(hua)(hua)鈉(na), pH 7.6防腐(fu)劑（Preservative）0.05%疊氮化(hua)(hua)鈉(na)運輸與(yu)(yu)(yu)保(bao)(bao)存(cun)(cun)方法(fa)(fa)冰(bing)袋運輸。凍(dong)干(gan)粉保(bao)(bao)存(cun)(cun)于2～8℃。100%原(yuan)液(ye)(ye)保(bao)(bao)存(cun)(cun)于2～8℃，6個(ge)星期穩定。建(jian)議原(yuan)液(ye)(ye)分(fen)裝后(hou)(hou)(hou)置(zhi)(zhi)(zhi)于≤-20℃，以(yi)(yi)延長保(bao)(bao)存(cun)(cun)期。重溶(rong)(rong)(rong)后(hou)(hou)(hou)100%原(yuan)液(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)效期1年。注(zhu)意(yi)事項1）本(ben)品(pin)(pin)含(han)疊氮化(hua)(hua)鈉(na)，對人體(ti)有(you)害，請(qing)(qing)注(zhu)意(yi)適當防護。2）為(wei)了(le)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)安全和(he)(he)健(jian)康(kang)，請(qing)(qing)穿實(shi)驗服并(bing)戴(dai)一次性(xing)手(shou)套操作(zuo)。3）注(zhu)意(yi)無(wu)(wu)菌(jun)(jun)(jun)操作(zuo)4）注(zhu)意(yi)避免(mian)反(fan)(fan)復(fu)凍(dong)融(rong)(rong)。反(fan)(fan)復(fu)凍(dong)融(rong)(rong)可(ke)(ke)能產(chan)(chan)生(sheng)(sheng)沉(chen)(chen)淀(dian)(dian)，可(ke)(ke)以(yi)(yi)通過(guo)(guo)(guo)無(wu)(wu)菌(jun)(jun)(jun)條件下，3500rpm離(li)(li)心(xin)10min除去沉(chen)(chen)淀(dian)(dian)標(biao)準(zhun)山(shan)(shan)羊(yang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)（細(xi)(xi)菌(jun)(jun)(jun)培養(yang)(yang)專用(yong)(yong)）常(chang)見問題 保(bao)(bao)存(cun)(cun)方法(fa)(fa)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)應(ying)保(bao)(bao)存(cun)(cun)在(zai)(zai)(zai)-5℃至(zhi)(zhi)-20℃。然(ran)而(er)(er)，若(ruo)(ruo)(ruo)存(cun)(cun)放于4℃時(shi)，請(qing)(qing)勿超過(guo)(guo)(guo)一個(ge)月(yue)。若(ruo)(ruo)(ruo)您一次無(wu)(wu)法(fa)(fa)用(yong)(yong)完一瓶，建(jian)議將(jiang)無(wu)(wu)菌(jun)(jun)(jun)分(fen)裝血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)至(zhi)(zhi)恰(qia)當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)滅(mie)(mie)菌(jun)(jun)(jun)容器內，再放回冷(leng)凍(dong)。解(jie)凍(dong)方法(fa)(fa)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)從(cong)冷(leng)凍(dong)箱取出后(hou)(hou)(hou)，先(xian)置(zhi)(zhi)(zhi)于2-8℃冰(bing)箱使(shi)(shi)(shi)之(zhi)溶(rong)(rong)(rong)解(jie)，然(ran)后(hou)(hou)(hou)在(zai)(zai)(zai)室(shi)溫下使(shi)(shi)(shi)之(zhi)全溶(rong)(rong)(rong)。但(dan)必(bi)須(xu)(xu)注(zhu)意(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)，溶(rong)(rong)(rong)解(jie)過(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)必(bi)須(xu)(xu)規則(ze)地搖晃(huang)均勻(yun)。避免(mian)產(chan)(chan)生(sheng)(sheng)沉(chen)(chen)淀(dian)(dian)物(wu)1、解(jie)凍(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)時(shi)，請(qing)(qing)按(an)照(zhao)所(suo)(suo)建(jian)議的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)逐步解(jie)凍(dong)法(fa)(fa)（-2℃至(zhi)(zhi)4℃至(zhi)(zhi)室(shi)溫），若(ruo)(ruo)(ruo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)解(jie)凍(dong)時(shi)改(gai)變(bian)(bian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)溫度(du)太(tai)大(da)(da)（如(ru)-20℃至(zhi)(zhi)37℃），實(shi)驗顯(xian)示(shi)非(fei)(fei)常(chang)容易產(chan)(chan)生(sheng)(sheng)沉(chen)(chen)淀(dian)(dian)物(wu)。2、解(jie)凍(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)時(shi)，請(qing)(qing)隨時(shi)將(jiang)之(zhi)搖晃(huang)均勻(yun)，使(shi)(shi)(shi)溫度(du)及成分(fen)均一，減少沉(chen)(chen)淀(dian)(dian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)生(sheng)(sheng)。3、請(qing)(qing)勿將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)置(zhi)(zhi)(zhi)于37℃太(tai)久(jiu)。若(ruo)(ruo)(ruo)在(zai)(zai)(zai)37℃放置(zhi)(zhi)(zhi)太(tai)久(jiu)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)會(hui)變(bian)(bian)得(de)混(hun)濁(zhuo)，同(tong)時(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)中(zhong)(zhong)(zhong)許多(duo)較不(bu)穩定的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成分(fen)也(ye)會(hui)因(yin)此(ci)(ci)受到(dao)(dao)(dao)損(sun)害，而(er)(er)影響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質量。4、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱滅(mie)(mie)活(huo)非(fei)(fei)常(chang)容易造(zao)成沉(chen)(chen)淀(dian)(dian)物(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)增(zeng)多(duo)，若(ruo)(ruo)(ruo)非(fei)(fei)必(bi)要(yao)，可(ke)(ke)以(yi)(yi)無(wu)(wu)須(xu)(xu)做此(ci)(ci)步驟(zou)。 5、若(ruo)(ruo)(ruo)必(bi)須(xu)(xu)做血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱滅(mie)(mie)活(huo)，請(qing)(qing)遵守56℃，30分(fen)鐘(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)則(ze)，并(bing)且隨時(shi)搖晃(huang)均勻(yun)。溫度(du)過(guo)(guo)(guo)高，時(shi)間(jian)過(guo)(guo)(guo)久(jiu)或(huo)搖晃(huang)不(bu)均勻(yun)，都(dou)(dou)會(hui)造(zao)成沉(chen)(chen)淀(dian)(dian)物(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)增(zeng)多(duo)。若(ruo)(ruo)(ruo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)解(jie)凍(dong)后(hou)(hou)(hou)發(fa)現有(you)絮(xu)狀沉(chen)(chen)淀(dian)(dian)物(wu)出現，可(ke)(ke)以(yi)(yi)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)分(fen)裝至(zhi)(zhi)無(wu)(wu)菌(jun)(jun)(jun)離(li)(li)心(xin)管(guan)內，以(yi)(yi)400g稍微離(li)(li)心(xin)，上清(qing)(qing)液(ye)(ye)即可(ke)(ke)接著加(jia)入培養(yang)(yang)基內一起(qi)過(guo)(guo)(guo)濾。但(dan)不(bu)建(jian)議以(yi)(yi)過(guo)(guo)(guo)濾的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方法(fa)(fa)去除這些(xie)絮(xu)狀沉(chen)(chen)淀(dian)(dian)物(wu)，因(yin)為(wei)它可(ke)(ke)能會(hui)阻(zu)塞(sai)過(guo)(guo)(guo)濾膜。熱滅(mie)(mie)活(huo)一般是(shi)(shi)以(yi)(yi)56℃，30分(fen)鐘(zhong)來處(chu)理已解(jie)凍(dong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)，因(yin)為(wei)此(ci)(ci)加(jia)熱步驟(zou)，可(ke)(ke)以(yi)(yi)使(shi)(shi)(shi)補體(ti)去活(huo)化(hua)(hua)，而(er)(er)補體(ti)所(suo)(suo)參與(yu)(yu)(yu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)反(fan)(fan)應(ying)有(you)：溶(rong)(rong)(rong)細(xi)(xi)胞(bao)活(huo)性(xing)，平滑肌(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)收縮(suo)，肥大(da)(da)細(xi)(xi)胞(bao)和(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小板組胺的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)釋(shi)放，增(zeng)強吞噬作(zuo)用(yong)(yong)，淋(lin)巴細(xi)(xi)胞(bao)、巨噬細(xi)(xi)胞(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)趨化(hua)(hua)和(he)(he)激活(huo)。鑒別方法(fa)(fa) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)凝(ning)(ning)(ning)(ning)(ning)(ning)固(gu)析出的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)淡(dan)(dan)黃色透明液(ye)(ye)體(ti) 。如(ru)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)自血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管(guan)內抽出，放入試管(guan)中(zhong)(zhong)(zhong)，不(bu)加(jia)抗(kang)凝(ning)(ning)(ning)(ning)(ning)(ning)劑，則(ze)凝(ning)(ning)(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)(fan)應(ying)被(bei)激活(huo)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)迅速凝(ning)(ning)(ning)(ning)(ning)(ning)固(gu)，形(xing)成膠(jiao)凍(dong)。凝(ning)(ning)(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)塊收縮(suo)，其(qi)周圍所(suo)(suo)析出之(zhi)淡(dan)(dan)黃色透明液(ye)(ye)體(ti)即為(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)，也(ye)可(ke)(ke)于凝(ning)(ning)(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后(hou)(hou)(hou)經(jing)離(li)(li)心(xin)取得(de)。在(zai)(zai)(zai)凝(ning)(ning)(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)過(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)，纖維(wei)(wei)蛋(dan)(dan)白(bai)(bai)原(yuan)轉變(bian)(bian)成纖維(wei)(wei)蛋(dan)(dan)白(bai)(bai)塊，所(suo)(suo)以(yi)(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)中(zhong)(zhong)(zhong)無(wu)(wu)纖維(wei)(wei)蛋(dan)(dan)白(bai)(bai)原(yuan)，這一點是(shi)(shi)與(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)＊大(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別。而(er)(er)在(zai)(zai)(zai)凝(ning)(ning)(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)(fan)應(ying)中(zhong)(zhong)(zhong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小板釋(shi)放出許多(duo)物(wu)質，各凝(ning)(ning)(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)因(yin)子也(ye)都(dou)(dou)發(fa)生(sheng)(sheng)了(le)變(bian)(bian)化(hua)(hua)。這些(xie)成分(fen)都(dou)(dou)留在(zai)(zai)(zai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)中(zhong)(zhong)(zhong)并(bing)繼續發(fa)生(sheng)(sheng)變(bian)(bian)化(hua)(hua)，如(ru)凝(ning)(ning)(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)酶原(yuan)變(bian)(bian)成凝(ning)(ning)(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)酶，并(bing)隨血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)存(cun)(cun)放時(shi)間(jian)逐漸減少以(yi)(yi)至(zhi)(zhi)消失。這些(xie)也(ye)都(dou)(dou)是(shi)(shi)與(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)區別之(zhi)處(chu)。但(dan)大(da)(da)量未參加(jia)凝(ning)(ning)(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)(fan)應(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)質則(ze)與(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)基本(ben)相同(tong)。為(wei)避免(mian)抗(kang)凝(ning)(ning)(ning)(ning)(ning)(ning)劑的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)干(gan)擾，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)中(zhong)(zhong)(zhong)許多(duo)化(hua)(hua)學成分(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)析，都(dou)(dou)以(yi)(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)為(wei)樣品(pin)(pin)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)體(ti)成分(fen)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)胞(bao)懸濁(zhuo)于其(qi)中(zhong)(zhong)(zhong)。人體(ti)含(han)有(you)2750-3300毫升血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)，約占血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)總體(ti)積的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)55%。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)絕大(da)(da)部分(fen)是(shi)(shi)水(shui)（體(ti)積的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)90%），其(qi)中(zhong)(zhong)(zhong)溶(rong)(rong)(rong)解(jie)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)質主要(yao)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)蛋(dan)(dan)白(bai)(bai)，還包括(kuo)葡萄(tao)糖、無(wu)(wu)機鹽離(li)(li)子、激素以(yi)(yi)及二氧化(hua)(hua)碳。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)功能是(shi)(shi)運載血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)胞(bao)，同(tong)時(shi)也(ye)是(shi)(shi)運輸分(fen)泌產(chan)(chan)物(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)媒(mei)介。將(jiang)新鮮血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)離(li)(li)心(xin)，使(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)胞(bao)沉(chen)(chen)降，上層淡(dan)(dan)黃色清(qing)(qing)液(ye)(ye)即是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)與(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)中(zhong)(zhong)(zhong)不(bu)含(han)纖維(wei)(wei)蛋(dan)(dan)白(bai)(bai)原(yuan)等(deng)凝(ning)(ning)(ning)(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)因(yin)子。
標(biao)準(zhun)馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)專用(yong)）產(chan)(chan)品介紹(shao) 采用(yong)健康(kang)動(dong)(dong)物(wu)(wu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)為(wei)原(yuan)(yuan)(yuan)料，經(jing)無(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)采集(ji)，分(fen)(fen)離，微孔(kong)過(guo)(guo)濾后而成(cheng)(cheng)，性(xing)(xing)(xing)狀為(wei)橙(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)體(ti)，無(wu)(wu)(wu)(wu)噬菌(jun)(jun)(jun)(jun)體(ti)，無(wu)(wu)(wu)(wu)溶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)，無(wu)(wu)(wu)(wu)異物(wu)(wu)，無(wu)(wu)(wu)(wu)細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)，真菌(jun)(jun)(jun)(jun)支(zhi)(zhi)(zhi)原(yuan)(yuan)(yuan)體(ti)毒形(xing)體(ti)衣原(yuan)(yuan)(yuan)體(ti)等，適應(ying)(ying)于(yu)(yu)試(shi)驗(yan)室或生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)科(ke)研(yan)相(xiang)關(guan)試(shi)驗(yan)，滿足不(bu)同科(ke)研(yan)實驗(yan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)多(duo)種(zhong)(zhong)需求注(zhu)意(yi)(yi)事項1、注(zhu)意(yi)(yi)避免(mian)反(fan)復凍(dong)融。反(fan)復凍(dong)融可(ke)能產(chan)(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)沉(chen)淀，可(ke)以通過(guo)(guo)無(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)條件下(xia)，3500 rp m離心10 min除去沉(chen)淀。2、注(zhu)意(yi)(yi)無(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)操作(zuo)(zuo)。標(biao)簽：馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)說明(ming)書(shu) ；馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)規格產(chan)(chan)品用(yong)途1、可(ke)用(yong)于(yu)(yu)細(xi)(xi)(xi)胞(bao)(bao)(bao)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)或誘導樹突細(xi)(xi)(xi)胞(bao)(bao)(bao)，且細(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)，增(zeng)(zeng)殖(zhi)狀況良好 有(you)(you)研(yan)究人員分(fen)(fen)別(bie)用(yong)胎(tai)(tai)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)和(he)(he)(he)馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)馬(ma)(ma)(ma)(ma)(ma)單(dan)(dan)核細(xi)(xi)(xi)胞(bao)(bao)(bao)來(lai)產(chan)(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)權突狀細(xi)(xi)(xi)胞(bao)(bao)(bao)（eqMoDC），結(jie)果(guo)發(fa)(fa)(fa)現(xian)，馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)生(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)eqMoDC與(yu)FBS生(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)eqMoDC沒有(you)(you)區別(bie)。然(ran)而，與(yu)馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)補充(chong)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基一起(qi)孵(fu)育(yu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)e qMo DC在(zai)(zai)(zai)從單(dan)(dan)核細(xi)(xi)(xi)胞(bao)(bao)(bao)分(fen)(fen)化(hua)過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)表現(xian)出(chu)(chu)更(geng)(geng)具特(te)征性(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樹突狀細(xi)(xi)(xi)胞(bao)(bao)(bao)形(xing)態。在(zai)(zai)(zai)用(yong)馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)eqMoDC中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)也觀(guan)察到(dao)(dao)細(xi)(xi)(xi)胞(bao)(bao)(bao)活力的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)顯(xian)著增(zeng)(zeng)加。此(ci)外(wai)，發(fa)(fa)(fa)現(xian)在(zai)(zai)(zai)馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)存(cun)在(zai)(zai)(zai)下(xia)產(chan)(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)eqMoDC在(zai)(zai)(zai)功能性(xing)(xing)(xing)T淋巴細(xi)(xi)(xi)胞(bao)(bao)(bao)啟動(dong)(dong)能力方(fang)面優越，并且引發(fa)(fa)(fa)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)非特(te)異性(xing)(xing)(xing)增(zeng)(zeng)殖(zhi)顯(xian)著減少。2、可(ke)用(yong)于(yu)(yu)原(yuan)(yuan)(yuan)代(dai)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)神(shen)經(jing)細(xi)(xi)(xi)胞(bao)(bao)(bao) 有(you)(you)人用(yong)含(han)10%馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)DMEM，分(fen)(fen)離培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)BALB/c小(xiao)鼠原(yuan)(yuan)(yuan)代(dai)腦神(shen)經(jing)細(xi)(xi)(xi)胞(bao)(bao)(bao)。發(fa)(fa)(fa)現(xian)和(he)(he)(he)添加2%神(shen)經(jing)細(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)劑B27的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)Neurobasal培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基相(xiang)比，含(han)10%馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)DMEM培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基更(geng)(geng)節省成(cheng)(cheng)本(ben)，方(fang)便廉價。3、特(te)定脂肪(fang)酸(suan)產(chan)(chan)物(wu)(wu)濃(nong)度的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),對造(zao)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)(xi)胞(bao)(bao)(bao)有(you)(you)支(zhi)(zhi)(zhi)持作(zuo)(zuo)用(yong) 研(yan)究表明(ming)，磷脂酶(mei)A2特(te)定脂肪(fang)酸(suan)產(chan)(chan)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)濃(nong)度馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，支(zhi)(zhi)(zhi)持多(duo)能小(xiao)鼠造(zao)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)祖(zu)細(xi)(xi)(xi)胞(bao)(bao)(bao)FDCP-Mix細(xi)(xi)(xi)胞(bao)(bao)(bao)自我更(geng)(geng)新的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)能力。4、可(ke)增(zeng)(zeng)強豬(zhu)單(dan)(dan)性(xing)(xing)(xing)生(sheng)(sheng)(sheng)(sheng)(sheng)殖(zhi)胚(pei)胎(tai)(tai)植入前(qian)，胚(pei)層(ceng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)(fa)(fa)育(yu)。 有(you)(you)研(yan)究通過(guo)(guo)對比不(bu)同血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)和(he)(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)樣(yang)(yang)物(wu)(wu)質(zhi)(zhi)(zhi)，對豬(zhu)單(dan)(dan)性(xing)(xing)(xing)生(sheng)(sheng)(sheng)(sheng)(sheng)殖(zhi)胚(pei)胎(tai)(tai)植入前(qian)發(fa)(fa)(fa)育(yu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)影響，尋找適合其(qi)(qi)發(fa)(fa)(fa)育(yu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基。對單(dan)(dan)性(xing)(xing)(xing)生(sheng)(sheng)(sheng)(sheng)(sheng)殖(zhi)胚(pei)胎(tai)(tai)進行豬(zhu)濾泡液(ye)(ye)(ye)（PFF)、胎(tai)(tai)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(（FBS)、馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（HS）或豬(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)白(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)（PSA)處理，培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)2天(tian)后。通過(guo)(guo)囊(nang)胚(pei)形(xing)成(cheng)(cheng)和(he)(he)(he)孵(fu)化(hua)，發(fa)(fa)(fa)現(xian)馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)增(zeng)(zeng)強胚(pei)層(ceng)發(fa)(fa)(fa)育(yu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)＊有(you)(you)效蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)來(lai)源。接下(xia)來(lai)，使(shi)用(yong)三種(zhong)(zhong)不(bu)同濃(nong)度的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)HS (10%，20%和(he)(he)(he)30%）來(lai)確(que)定改善豬(zhu)單(dan)(dan)性(xing)(xing)(xing)生(sheng)(sheng)(sheng)(sheng)(sheng)殖(zhi)胚(pei)胎(tai)(tai)發(fa)(fa)(fa)育(yu)所需的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)適宜HS濃(nong)度。所有(you)(you)HS濃(nong)度均增(zeng)(zeng)加了囊(nang)胚(pei)細(xi)(xi)(xi)胞(bao)(bao)(bao)數量，降低了囊(nang)胚(pei)凋亡細(xi)(xi)(xi)胞(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)率(lv)，其(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)20%效果(guo)更(geng)(geng)顯(xian)著。5、馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)添加到(dao)(dao)微生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，可(ke)用(yong)于(yu)(yu)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)微生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)，如(ru)(ru)(ru)∶(1）馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)添加到(dao)(dao)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)用(yong)于(yu)(yu)支(zhi)(zhi)(zhi)原(yuan)(yuan)(yuan)體(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)。在(zai)(zai)(zai)《獸(shou)藥典》中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，含(han)馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)支(zhi)(zhi)(zhi)原(yuan)(yuan)(yuan)體(ti)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基被用(yong)于(yu)(yu)獸(shou)用(yong)疫苗的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)支(zhi)(zhi)(zhi)原(yuan)(yuan)(yuan)體(ti)檢(jian)查。用(yong)KM2培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(含(han)馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing))還可(ke)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)豬(zhu)肺炎(yan)支(zhi)(zhi)(zhi)原(yuan)(yuan)(yuan)體(ti)。(2）馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)加入到(dao)(dao)固體(ti)和(he)(he)(he)液(ye)(ye)(ye)體(ti)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，可(ke)用(yong)于(yu)(yu)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)幽門螺桿菌(jun)(jun)(jun)(jun)。并且，和(he)(he)(he)羊血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)和(he)(he)(he)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)比較，添加馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基更(geng)(geng)易于(yu)(yu)分(fen)(fen)裝(zhuang)和(he)(he)(he)保(bao)存(cun)。標(biao)準(zhun)馬(ma)(ma)(ma)(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（細(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)專用(yong)）檢(jian)驗(yan)報(bao)告(gao)：檢(jian)測項目檢(jian)測結(jie)果(guo)外(wai)觀(guan)淡黃色(se)(se)液(ye)(ye)(ye)體(ti)PH7.26無(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)檢(jian)驗(yan)陰性(xing)(xing)(xing)總蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)68.6g/L白(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)35.1g/L支(zhi)(zhi)(zhi)原(yuan)(yuan)(yuan)體(ti)陰性(xing)(xing)(xing)內毒素(su)8UE/mL血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)紅蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)0.04g/L血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)要(yao)作(zuo)(zuo)用(yong)●提(ti)供基本(ben)營養(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)質(zhi)(zhi)(zhi)：氨基酸(suan)、維(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)素(su)、無(wu)(wu)(wu)(wu)機(ji)(ji)物(wu)(wu)、脂類物(wu)(wu)質(zhi)(zhi)(zhi)、核酸(suan)衍生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)等，是(shi)細(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)必須的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)質(zhi)(zhi)(zhi)。●提(ti)供激(ji)素(su)和(he)(he)(he)各種(zhong)(zhong)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)子(zi)：胰(yi)島素(su)、腎上腺(xian)皮(pi)質(zhi)(zhi)(zhi)激(ji)素(su)（氫化(hua)可(ke)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)松(song)、地塞(sai)米(mi)松(song)）、類固醇激(ji)素(su)（雌二醇、睪酮(tong)、孕(yun)酮(tong)）等。生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)子(zi)如(ru)(ru)(ru)成(cheng)(cheng)纖(xian)維(wei)細(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)子(zi)、表皮(pi)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)子(zi)、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)板(ban)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)子(zi)等。●提(ti)供結(jie)合蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)：結(jie)合蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)作(zuo)(zuo)用(yong)是(shi)攜(xie)帶重要(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低分(fen)(fen)子(zi)量物(wu)(wu)質(zhi)(zhi)(zhi)，如(ru)(ru)(ru)白(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)攜(xie)帶維(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)素(su)、脂肪(fang)、以及激(ji)素(su)等，轉鐵蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)攜(xie)帶鐵。結(jie)合蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)在(zai)(zai)(zai)細(xi)(xi)(xi)胞(bao)(bao)(bao)代(dai)謝(xie)過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)起(qi)重要(yao)作(zuo)(zuo)用(yong)。●提(ti)供促接觸(chu)和(he)(he)(he)伸(shen)展(zhan)因(yin)子(zi)使(shi)細(xi)(xi)(xi)胞(bao)(bao)(bao)貼壁免(mian)受機(ji)(ji)械(xie)損傷。●對培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)胞(bao)(bao)(bao)起(qi)到(dao)(dao)某些(xie)(xie)保(bao)護作(zuo)(zuo)用(yong)：有(you)(you)一些(xie)(xie)細(xi)(xi)(xi)胞(bao)(bao)(bao)，如(ru)(ru)(ru)內皮(pi)細(xi)(xi)(xi)胞(bao)(bao)(bao)、骨髓樣(yang)(yang)細(xi)(xi)(xi)胞(bao)(bao)(bao)可(ke)以釋放蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)酶(mei)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)含(han)有(you)(you)抗蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)酶(mei)成(cheng)(cheng)分(fen)(fen)，起(qi)到(dao)(dao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)作(zuo)(zuo)用(yong)。這(zhe)種(zhong)(zhong)作(zuo)(zuo)用(yong)是(shi)偶然(ran)發(fa)(fa)(fa)現(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，則有(you)(you)目的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)使(shi)用(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)來(lai)終止胰(yi)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)酶(mei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消化(hua)作(zuo)(zuo)用(yong)。因(yin)為(wei)胰(yi)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)酶(mei)已經(jing)被廣(guang)泛(fan)用(yong)于(yu)(yu)貼壁細(xi)(xi)(xi)胞(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消化(hua)傳代(dai)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)形(xing)成(cheng)(cheng)了血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)粘度，可(ke)以保(bao)護細(xi)(xi)(xi)胞(bao)(bao)(bao)免(mian)受機(ji)(ji)械(xie)損傷，特(te)別(bie)是(shi)在(zai)(zai)(zai)懸浮培(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)攪拌時，粘度起(qi)到(dao)(dao)重要(yao)作(zuo)(zuo)用(yong)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)還含(han)有(you)(you)一些(xie)(xie)微量元素(su)和(he)(he)(he)離子(zi)，他們在(zai)(zai)(zai)代(dai)謝(xie)解(jie)毒中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)起(qi)重要(yao)作(zuo)(zuo)用(yong)，如(ru)(ru)(ru)SeO3，硒等。鑒別(bie)方(fang)法(fa) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)凝(ning)固析出(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)淡黃色(se)(se)透(tou)明(ming)液(ye)(ye)(ye)體(ti) 。如(ru)(ru)(ru)將血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)自血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管內抽(chou)出(chu)(chu)，放入試(shi)管中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，不(bu)加抗凝(ning)劑，則凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)應(ying)(ying)被激(ji)活，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)迅(xun)速凝(ning)固，形(xing)成(cheng)(cheng)膠凍(dong)。凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)塊收縮，其(qi)(qi)周圍所析出(chu)(chu)之(zhi)淡黃色(se)(se)透(tou)明(ming)液(ye)(ye)(ye)體(ti)即為(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，也可(ke)于(yu)(yu)凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后經(jing)離心取(qu)得。在(zai)(zai)(zai)凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，纖(xian)維(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)轉變(bian)成(cheng)(cheng)纖(xian)維(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)塊，所以血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)無(wu)(wu)(wu)(wu)纖(xian)維(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)，這(zhe)一點(dian)是(shi)與(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)＊大(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie)。而在(zai)(zai)(zai)凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)應(ying)(ying)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)板(ban)釋放出(chu)(chu)許(xu)多(duo)物(wu)(wu)質(zhi)(zhi)(zhi)，各凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)因(yin)子(zi)也都(dou)發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)了變(bian)化(hua)。這(zhe)些(xie)(xie)成(cheng)(cheng)分(fen)(fen)都(dou)留在(zai)(zai)(zai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)并繼續發(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)變(bian)化(hua)，如(ru)(ru)(ru)凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)酶(mei)原(yuan)(yuan)(yuan)變(bian)成(cheng)(cheng)凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)酶(mei)，并隨(sui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)存(cun)放時間(jian)逐漸減少以至消失。這(zhe)些(xie)(xie)也都(dou)是(shi)與(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)區別(bie)之(zhi)處。但大(da)量未(wei)參加凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)應(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)質(zhi)(zhi)(zhi)則與(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)基本(ben)相(xiang)同。為(wei)避免(mian)抗凝(ning)劑的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)干(gan)擾(rao)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)許(xu)多(duo)化(hua)學成(cheng)(cheng)分(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)析，都(dou)以血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)樣(yang)(yang)品。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)是(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)體(ti)成(cheng)(cheng)分(fen)(fen)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)(xi)胞(bao)(bao)(bao)懸濁(zhuo)于(yu)(yu)其(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。人體(ti)含(han)有(you)(you)2750-3300毫(hao)升血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)，約占(zhan)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)總體(ti)積(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)55%。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)絕大(da)部分(fen)(fen)是(shi)水(shui)（體(ti)積(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)90%），其(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)溶解(jie)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)質(zhi)(zhi)(zhi)主(zhu)(zhu)要(yao)是(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)，還包(bao)括葡萄糖、無(wu)(wu)(wu)(wu)機(ji)(ji)鹽離子(zi)、激(ji)素(su)以及二氧化(hua)碳。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)要(yao)功能是(shi)運載(zai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)(xi)胞(bao)(bao)(bao)，同時也是(shi)運輸分(fen)(fen)泌產(chan)(chan)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)要(yao)媒介。
標(biao)準小(xiao)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)（細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)專用(yong)(yong)(yong)） 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)礎(chu)(chu)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)中(zhong)(zhong)極(ji)為(wei)(wei)(wei)(wei)重(zhong)(zhong)要的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)和(he)(he)(he)(he)(he)(he)粘附因子(zi)、激(ji)素(su)(su)、脂(zhi)(zhi)質(zhi)(zhi)和(he)(he)(he)(he)(he)(he)礦物(wu)(wu)(wu)(wu)質(zhi)(zhi)來源(yuan)。此(ci)外(wai)(wai)(wai)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)還可(ke)(ke)(ke)(ke)(ke)調節細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)膜通(tong)透性(xing)(xing)(xing)，并(bing)(bing)可(ke)(ke)(ke)(ke)(ke)作(zuo)為(wei)(wei)(wei)(wei)向細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)內(nei)運送脂(zhi)(zhi)質(zhi)(zhi)、酶、微量(liang)(liang)(liang)(liang)(liang)營養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)素(su)(su)和(he)(he)(he)(he)(he)(he)微量(liang)(liang)(liang)(liang)(liang)元素(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)載體(ti)(ti)(ti)(ti)(ti)。牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)用(yong)(yong)(yong)量(liang)(liang)(liang)(liang)(liang)天然(ran)(ran)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，含(han)(han)(han)有(you)(you)(you)豐(feng)富(fu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)必(bi)(bi)須的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)營養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)成(cheng)(cheng)(cheng)份，具(ju)(ju)有(you)(you)(you)重(zhong)(zhong)要的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)功能(neng)。牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)分(fen)(fen)為(wei)(wei)(wei)(wei)小(xiao)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)、新牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)、胎牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)。 產(chan)品簡介(jie) 牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)一(yi)(yi)種(zhong)(zhong)(zhong)(zhong)(zhong)性(xing)(xing)(xing)狀、外(wai)(wai)(wai)觀(guan)(guan) 淺(qian)黃色(se)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)、無(wu)(wu)溶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)、無(wu)(wu)異物(wu)(wu)(wu)(wu)稍(shao)粘稠液(ye)(ye)體(ti)(ti)(ti)(ti)(ti)。胎牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)應(ying)(ying)(ying)(ying)(ying)取(qu)(qu)自(zi)(zi)剖(pou)腹(fu)產(chan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)胎牛；新牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)取(qu)(qu)自(zi)(zi)出(chu)(chu)生(sheng)(sheng)(sheng)24小(xiao)時(shi)(shi)(shi)之(zhi)(zhi)內(nei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)新生(sheng)(sheng)(sheng)牛；小(xiao)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)取(qu)(qu)自(zi)(zi)出(chu)(chu)生(sheng)(sheng)(sheng)10－30天的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)小(xiao)牛。1、性(xing)(xing)(xing)狀、外(wai)(wai)(wai)觀(guan)(guan) 淺(qian)黃色(se)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)、無(wu)(wu)溶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)、無(wu)(wu)異物(wu)(wu)(wu)(wu)稍(shao)粘稠液(ye)(ye)體(ti)(ti)(ti)(ti)(ti)。2、蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)(zhi)含(han)(han)(han)量(liang)(liang)(liang)(liang)(liang) 3.5%～5.0%（ w/v）3、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)紅蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)含(han)(han)(han)量(liang)(liang)(liang)(liang)(liang) ≤ 0.02%（ w/v）4、無(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)檢(jian)驗 陰(yin)性(xing)(xing)(xing)5、支原(yuan)體(ti)(ti)(ti)(ti)(ti)檢(jian)驗 陰(yin)性(xing)(xing)(xing)6、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)內(nei)毒(du)(du)(du)(du)素(su)(su) ≤5（EU/ml）7、牛腹(fu)瀉病(bing)(bing)毒(du)(du)(du)(du) 陰(yin)性(xing)(xing)(xing)8、大腸桿菌(jun)(jun)(jun)(jun)(jun)噬菌(jun)(jun)(jun)(jun)(jun)體(ti)(ti)(ti)(ti)(ti) 陰(yin)性(xing)(xing)(xing)9、支持(chi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)增殖(Sp2/0-Ag14) 生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)曲線(xian)（接(jie)(jie)種(zhong)(zhong)(zhong)(zhong)(zhong)濃度）＊大增殖濃度≥2.5×106 個/ml細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)倍增時(shi)(shi)(shi)間 ≤15h克隆率 ≥85%細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang) 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)是(shi)(shi)(shi)(shi)(shi)一(yi)(yi)種(zhong)(zhong)(zhong)(zhong)(zhong)用(yong)(yong)(yong)人(ren)(ren)工方(fang)(fang)法(fa)使(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)繁殖的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)技(ji)術(shu)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)在(zai)(zai)(zai)(zai)(zai)自(zi)(zi)然(ran)(ran)界中(zhong)(zhong)分(fen)(fen)布極(ji)廣，數量(liang)(liang)(liang)(liang)(liang)大，種(zhong)(zhong)(zhong)(zhong)(zhong)類多，它可(ke)(ke)(ke)(ke)(ke)以(yi)造(zao)福人(ren)(ren)類，也可(ke)(ke)(ke)(ke)(ke)以(yi)成(cheng)(cheng)(cheng)為(wei)(wei)(wei)(wei)致病(bing)(bing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)因。大多數細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)可(ke)(ke)(ke)(ke)(ke)用(yong)(yong)(yong)人(ren)(ren)工方(fang)(fang)法(fa)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)，即(ji)將其(qi)接(jie)(jie)種(zhong)(zhong)(zhong)(zhong)(zhong)于(yu)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)上(shang)，使(shi)(shi)其(qi)生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)繁殖。培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)出(chu)(chu)來的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)用(yong)(yong)(yong)于(yu)研(yan)究、鑒定和(he)(he)(he)(he)(he)(he)應(ying)(ying)(ying)(ying)(ying)用(yong)(yong)(yong)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)是(shi)(shi)(shi)(shi)(shi)一(yi)(yi)個復(fu)雜的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)技(ji)術(shu)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)條(tiao)件(jian) 培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)應(ying)(ying)(ying)(ying)(ying)根據細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)類和(he)(he)(he)(he)(he)(he)目的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)等(deng)選(xuan)擇(ze)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)方(fang)(fang)法(fa)、培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，制(zhi)定培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)條(tiao)件(jian)(溫度、pH值、時(shi)(shi)(shi)間，對(dui)氧的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)需(xu)求與否(fou)等(deng))。一(yi)(yi)般操作(zuo)步(bu)(bu)驟為(wei)(wei)(wei)(wei)先將標(biao)本(ben)接(jie)(jie)種(zhong)(zhong)(zhong)(zhong)(zhong)于(yu)固體(ti)(ti)(ti)(ti)(ti)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)上(shang)，做分(fen)(fen)離(li)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。再進(jin)(jin)一(yi)(yi)步(bu)(bu)對(dui)所(suo)得單個菌(jun)(jun)(jun)(jun)(jun)落進(jin)(jin)行(xing)形態、生(sheng)(sheng)(sheng)化(hua)及(ji)(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)學反應(ying)(ying)(ying)(ying)(ying)鑒定。培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)常(chang)用(yong)(yong)(yong)牛肉(rou)湯、蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)胨(dong)、氯化(hua)鈉、葡萄糖、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)等(deng)和(he)(he)(he)(he)(he)(he)某些(xie)(xie)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)所(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)特殊物(wu)(wu)(wu)(wu)質(zhi)(zhi)配(pei)制(zhi)成(cheng)(cheng)(cheng)液(ye)(ye)體(ti)(ti)(ti)(ti)(ti)、半(ban)固體(ti)(ti)(ti)(ti)(ti)、固體(ti)(ti)(ti)(ti)(ti)等(deng)。一(yi)(yi)般細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)可(ke)(ke)(ke)(ke)(ke)在(zai)(zai)(zai)(zai)(zai)有(you)(you)(you)氧條(tiao)件(jian)下，37℃中(zhong)(zhong)放18~24小(xiao)時(shi)(shi)(shi)生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)。厭氧菌(jun)(jun)(jun)(jun)(jun)則(ze)(ze)需(xu)在(zai)(zai)(zai)(zai)(zai)無(wu)(wu)氧環境中(zhong)(zhong)放2~3天后(hou)生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)。個別細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)如結(jie)(jie)(jie)核(he)菌(jun)(jun)(jun)(jun)(jun)要培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)1個月(yue)之(zhi)(zhi)久。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)步(bu)(bu)驟 以(yi)光(guang)合(he)(he)(he)(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)方(fang)(fang)法(fa)為(wei)(wei)(wei)(wei)例(li)。光(guang)合(he)(he)(he)(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)法(fa)，按(an)(an)次(ci)序分(fen)(fen)為(wei)(wei)(wei)(wei)容(rong)器、工具(ju)(ju)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消(xiao)毒(du)(du)(du)(du)，培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)備(bei)，接(jie)(jie)種(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)管理(li)(li)(li)四個步(bu)(bu)驟。(一(yi)(yi))容(rong)器、工具(ju)(ju)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消(xiao)毒(du)(du)(du)(du)參考(kao)、此(ci)處(chu)(chu)從略。(二(er))培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)備(bei)1.培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)用(yong)(yong)(yong)水如果培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)合(he)(he)(he)(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)是(shi)(shi)(shi)(shi)(shi)淡(dan)水種(zhong)(zhong)(zhong)(zhong)(zhong)，菌(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)可(ke)(ke)(ke)(ke)(ke)用(yong)(yong)(yong)蒸(zheng)餾水，生(sheng)(sheng)(sheng)產(chan)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)可(ke)(ke)(ke)(ke)(ke)用(yong)(yong)(yong)消(xiao)毒(du)(du)(du)(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)自(zi)(zi)來水(或(huo)(huo)(huo)井水)配(pei)制(zhi)。如果培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)合(he)(he)(he)(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)是(shi)(shi)(shi)(shi)(shi)海水種(zhong)(zhong)(zhong)(zhong)(zhong)，則(ze)(ze)用(yong)(yong)(yong)天然(ran)(ran)海水配(pei)制(zhi)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，注意在(zai)(zai)(zai)(zai)(zai)海水中(zhong)(zhong)加(jia)(jia)入(ru)磷(lin)元素(su)(su)時(shi)(shi)(shi)，不(bu)(bu)(bu)(bu)能(neng)用(yong)(yong)(yong)磷(lin)酸(suan)氫(qing)二(er)鉀，應(ying)(ying)(ying)(ying)(ying)用(yong)(yong)(yong)磷(lin)酸(suan)二(er)氫(qing)鉀，不(bu)(bu)(bu)(bu)然(ran)(ran)會(hui)產(chan)生(sheng)(sheng)(sheng)大量(liang)(liang)(liang)(liang)(liang)沉淀(dian)。2.滅菌(jun)(jun)(jun)(jun)(jun)和(he)(he)(he)(he)(he)(he)消(xiao)毒(du)(du)(du)(du)菌(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)用(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)應(ying)(ying)(ying)(ying)(ying)連同培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)容(rong)器用(yong)(yong)(yong)高(gao)壓蒸(zheng)氣滅菌(jun)(jun)(jun)(jun)(jun)鍋(guo)滅菌(jun)(jun)(jun)(jun)(jun)。小(xiao)型生(sheng)(sheng)(sheng)產(chan)性(xing)(xing)(xing)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)可(ke)(ke)(ke)(ke)(ke)把(ba)(ba)配(pei)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)液(ye)(ye)用(yong)(yong)(yong)普通(tong)鋁鍋(guo)或(huo)(huo)(huo)大型三(san)角(jiao)燒瓶煮沸消(xiao)毒(du)(du)(du)(du)。大型生(sheng)(sheng)(sheng)產(chan)性(xing)(xing)(xing)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)則(ze)(ze)把(ba)(ba)經沉淀(dian)砂濾后(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)水用(yong)(yong)(yong)漂(piao)白(bai)(bai)(bai)(bai)粉(或(huo)(huo)(huo)漂(piao)白(bai)(bai)(bai)(bai)液(ye)(ye))消(xiao)毒(du)(du)(du)(du)后(hou)使(shi)(shi)用(yong)(yong)(yong)。3.培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)配(pei)制(zhi)根據所(suo)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)種(zhong)(zhong)(zhong)(zhong)(zhong)類的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)營養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)需(xu)要選(xuan)擇(ze)合(he)(he)(he)(he)(he)適(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)配(pei)方(fang)(fang)。按(an)(an)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)配(pei)方(fang)(fang)把(ba)(ba)所(suo)需(xu)物(wu)(wu)(wu)(wu)質(zhi)(zhi)稱量(liang)(liang)(liang)(liang)(liang)，逐一(yi)(yi)溶解，混合(he)(he)(he)(he)(he)，配(pei)成(cheng)(cheng)(cheng)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)。也可(ke)(ke)(ke)(ke)(ke)先配(pei)成(cheng)(cheng)(cheng)母液(ye)(ye)，使(shi)(shi)用(yong)(yong)(yong)時(shi)(shi)(shi)按(an)(an)比例(li)加(jia)(jia)入(ru)一(yi)(yi)定的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)(liang)(liang)即(ji)可(ke)(ke)(ke)(ke)(ke)。(三(san))接(jie)(jie)種(zhong)(zhong)(zhong)(zhong)(zhong)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)配(pei)好后(hou)，應(ying)(ying)(ying)(ying)(ying)立即(ji)進(jin)(jin)行(xing)接(jie)(jie)種(zhong)(zhong)(zhong)(zhong)(zhong)。光(guang)合(he)(he)(he)(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)生(sheng)(sheng)(sheng)產(chan)性(xing)(xing)(xing)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)按(an)(an)種(zhong)(zhong)(zhong)(zhong)(zhong)量(liang)(liang)(liang)(liang)(liang)比較高(gao)，一(yi)(yi)般為(wei)(wei)(wei)(wei)20%~50%，即(ji)菌(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)(zhong)母液(ye)(ye)量(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)(he)新配(pei)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)液(ye)(ye)雖之(zhi)(zhi)比為(wei)(wei)(wei)(wei)1∶4~1∶1，不(bu)(bu)(bu)(bu)應(ying)(ying)(ying)(ying)(ying)低(di)于(yu)20%，尤其(qi)是(shi)(shi)(shi)(shi)(shi)微氣培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)，接(jie)(jie)種(zhong)(zhong)(zhong)(zhong)(zhong)量(liang)(liang)(liang)(liang)(liang)更應(ying)(ying)(ying)(ying)(ying)高(gao)些(xie)(xie)，否(fou)則(ze)(ze)光(guang)合(he)(he)(he)(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)在(zai)(zai)(zai)(zai)(zai)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)液(ye)(ye)中(zhong)(zhong)很(hen)難(nan)占＊＊優勢(shi)，影(ying)響培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)＊終產(chan)量(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)(he)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)。(四)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)管理(li)(li)(li)光(guang)合(he)(he)(he)(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)過(guo)(guo)(guo)程中(zhong)(zhong)，管理(li)(li)(li)工作(zuo)包括日常(chang)管理(li)(li)(li)操作(zuo)和(he)(he)(he)(he)(he)(he)測(ce)試，生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)情況(kuang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)觀(guan)(guan)察、檢(jian)查以(yi)及(ji)(ji)出(chu)(chu)現(xian)問題的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)析處(chu)(chu)理(li)(li)(li)等(deng)三(san)個方(fang)(fang)面。標(biao)準小(xiao)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)（細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)專用(yong)(yong)(yong)）產(chan)品功能(neng) 牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)用(yong)(yong)(yong)量(liang)(liang)(liang)(liang)(liang)＊大的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)天然(ran)(ran)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，含(han)(han)(han)有(you)(you)(you)豐(feng)富(fu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)必(bi)(bi)須的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)營養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)成(cheng)(cheng)(cheng)份，常(chang)用(yong)(yong)(yong)于(yu)動物(wu)(wu)(wu)(wu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)體(ti)(ti)(ti)(ti)(ti)外(wai)(wai)(wai)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)，具(ju)(ju)有(you)(you)(you)極(ji)為(wei)(wei)(wei)(wei)重(zhong)(zhong)要的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)功能(neng)。1.提(ti)供(gong)對(dui)維持(chi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)指(zhi)(zhi)數生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)激(ji)素(su)(su)，基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)礎(chu)(chu)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)中(zhong)(zhong)沒有(you)(you)(you)或(huo)(huo)(huo)量(liang)(liang)(liang)(liang)(liang)很(hen)少的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)營養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)，以(yi)及(ji)(ji)主要的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低(di)分(fen)(fen)子(zi)營養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)。2. 提(ti)供(gong)結(jie)(jie)(jie)合(he)(he)(he)(he)(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)，能(neng)識別維生(sheng)(sheng)(sheng)素(su)(su)、脂(zhi)(zhi)類、金(jin)屬和(he)(he)(he)(he)(he)(he)其(qi)他(ta)激(ji)素(su)(su)等(deng)，能(neng)結(jie)(jie)(jie)合(he)(he)(he)(he)(he)或(huo)(huo)(huo)調變(bian)它們所(suo)結(jie)(jie)(jie)合(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)質(zhi)(zhi)活(huo)(huo)力。3.有(you)(you)(you)些(xie)(xie)情況(kuang)下結(jie)(jie)(jie)合(he)(he)(he)(he)(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)(zhi)能(neng)與有(you)(you)(you)毒(du)(du)(du)(du)金(jin)屬和(he)(he)(he)(he)(he)(he)熱(re)原(yuan)質(zhi)(zhi)結(jie)(jie)(jie)合(he)(he)(he)(he)(he)，起(qi)到(dao)解毒(du)(du)(du)(du)作(zuo)用(yong)(yong)(yong)。4.是(shi)(shi)(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)貼壁、鋪(pu)展在(zai)(zai)(zai)(zai)(zai)塑料(liao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)質(zhi)(zhi)上(shang)所(suo)需(xu)因子(zi)來源(yuan)。5.起(qi)酸(suan)堿度緩沖液(ye)(ye)作(zuo)用(yong)(yong)(yong)。6.提(ti)供(gong)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)酶抑制(zhi)劑(ji)，使(shi)(shi)在(zai)(zai)(zai)(zai)(zai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)傳代時(shi)(shi)(shi)使(shi)(shi)剩余胰蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)酶失活(huo)(huo)，保護細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)不(bu)(bu)(bu)(bu)受傷害。適(shi)用(yong)(yong)(yong)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)多種(zhong)(zhong)(zhong)(zhong)(zhong)腫瘤(liu)(liu)原(yuan)代和(he)(he)(he)(he)(he)(he)293、293T、CHO、SF9、Vero、Hela細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)系，各種(zhong)(zhong)(zhong)(zhong)(zhong)腫瘤(liu)(liu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)等(deng)保存(cun)(cun)條(tiao)件(jian)1. -20℃，五年2. 4oC保存(cun)(cun)通(tong)常(chang)不(bu)(bu)(bu)(bu)宜(yi)超過(guo)(guo)(guo)1個月(yue)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要成(cheng)(cheng)(cheng)分(fen)(fen) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)由血(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)去除(chu)(chu)(chu)纖維蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)而形成(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)種(zhong)(zhong)(zhong)(zhong)(zhong)很(hen)復(fu)雜的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)(he)(he)(he)(he)物(wu)(wu)(wu)(wu)，其(qi)組成(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)份雖大部(bu)分(fen)(fen)已知，但(dan)還有(you)(you)(you)一(yi)(yi)部(bu)分(fen)(fen)尚(shang)不(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)楚(chu)，且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)組成(cheng)(cheng)(cheng)及(ji)(ji)含(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)常(chang)隨(sui)供(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)動物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)(xing)別、年齡、生(sheng)(sheng)(sheng)理(li)(li)(li)條(tiao)件(jian)和(he)(he)(he)(he)(he)(he)營養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)條(tiao)件(jian)不(bu)(bu)(bu)(bu)同而異。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)含(han)(han)(han)有(you)(you)(you)各種(zhong)(zhong)(zhong)(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)、多肽、脂(zhi)(zhi)肪、碳水化(hua)合(he)(he)(he)(he)(he)物(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)因子(zi)、激(ji)素(su)(su)、無(wu)(wu)機物(wu)(wu)(wu)(wu)等(deng)，這些(xie)(xie)物(wu)(wu)(wu)(wu)質(zhi)(zhi)對(dui)促進(jin)(jin)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)或(huo)(huo)(huo)抑制(zhi)生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)活(huo)(huo)性(xing)(xing)(xing)是(shi)(shi)(shi)(shi)(shi)達到(dao)生(sheng)(sheng)(sheng)理(li)(li)(li)平衡的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。 牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)用(yong)(yong)(yong)量(liang)(liang)(liang)(liang)(liang)＊大的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)天然(ran)(ran)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)，含(han)(han)(han)有(you)(you)(you)豐(feng)富(fu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)必(bi)(bi)須的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)營養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)成(cheng)(cheng)(cheng)份，常(chang)用(yong)(yong)(yong)于(yu)動物(wu)(wu)(wu)(wu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)體(ti)(ti)(ti)(ti)(ti)外(wai)(wai)(wai)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)，具(ju)(ju)有(you)(you)(you)極(ji)為(wei)(wei)(wei)(wei)重(zhong)(zhong)要的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)功能(neng)。1.提(ti)供(gong)對(dui)維持(chi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)指(zhi)(zhi)數生(sheng)(sheng)(sheng)長(chang)(chang)(chang)(chang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)激(ji)素(su)(su)，基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)礎(chu)(chu)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)中(zhong)(zhong)沒有(you)(you)(you)或(huo)(huo)(huo)量(liang)(liang)(liang)(liang)(liang)很(hen)少的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)營養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)，以(yi)及(ji)(ji)主要的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低(di)分(fen)(fen)子(zi)營養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)。2. 提(ti)供(gong)結(jie)(jie)(jie)合(he)(he)(he)(he)(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)，能(neng)識別維生(sheng)(sheng)(sheng)素(su)(su)、脂(zhi)(zhi)類、金(jin)屬和(he)(he)(he)(he)(he)(he)其(qi)他(ta)激(ji)素(su)(su)等(deng)，能(neng)結(jie)(jie)(jie)合(he)(he)(he)(he)(he)或(huo)(huo)(huo)調變(bian)它們所(suo)結(jie)(jie)(jie)合(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)質(zhi)(zhi)活(huo)(huo)力。3.有(you)(you)(you)些(xie)(xie)情況(kuang)下結(jie)(jie)(jie)合(he)(he)(he)(he)(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)(zhi)能(neng)與有(you)(you)(you)毒(du)(du)(du)(du)金(jin)屬和(he)(he)(he)(he)(he)(he)熱(re)原(yuan)質(zhi)(zhi)結(jie)(jie)(jie)合(he)(he)(he)(he)(he)，起(qi)到(dao)解毒(du)(du)(du)(du)作(zuo)用(yong)(yong)(yong)。4.是(shi)(shi)(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)貼壁、鋪(pu)展在(zai)(zai)(zai)(zai)(zai)塑料(liao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)質(zhi)(zhi)上(shang)所(suo)需(xu)因子(zi)來源(yuan)。5.起(qi)酸(suan)堿度緩沖液(ye)(ye)作(zuo)用(yong)(yong)(yong)。6.提(ti)供(gong)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)酶抑制(zhi)劑(ji)，使(shi)(shi)在(zai)(zai)(zai)(zai)(zai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)傳代時(shi)(shi)(shi)使(shi)(shi)剩余胰蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)酶失活(huo)(huo)，保護細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)不(bu)(bu)(bu)(bu)受傷害。注意事項1. 融(rong)化(hua)方(fang)(fang)法(fa)：建(jian)議將血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)從-20℃冰(bing)箱(xiang)取(qu)(qu)出(chu)(chu)后(hou)，于(yu)2-8℃解凍(dong)過(guo)(guo)(guo)夜(ye)，并(bing)(bing)在(zai)(zai)(zai)(zai)(zai)此(ci)過(guo)(guo)(guo)程中(zhong)(zhong)不(bu)(bu)(bu)(bu)時(shi)(shi)(shi)輕(qing)輕(qing)搖動使(shi)(shi)之(zhi)(zhi)溶解均(jun)勻。2. 不(bu)(bu)(bu)(bu)宜(yi) 4℃甚(shen)至更高(gao)溫度長(chang)(chang)(chang)(chang)期保存(cun)(cun)，切(qie)忌反復(fu)凍(dong)融(rong)，小(xiao)量(liang)(liang)(liang)(liang)(liang)使(shi)(shi)用(yong)(yong)(yong)時(shi)(shi)(shi)建(jian)議分(fen)(fen)裝凍(dong)存(cun)(cun)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)結(jie)(jie)(jie)冰(bing)時(shi)(shi)(shi)體(ti)(ti)(ti)(ti)(ti)積會(hui)增加(jia)(jia)約10%，因此(ci)在(zai)(zai)(zai)(zai)(zai)分(fen)(fen)裝血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)(shi)須預留一(yi)(yi)定體(ti)(ti)(ti)(ti)(ti)積，否(fou)則(ze)(ze)易導(dao)致分(fen)(fen)裝瓶凍(dong)裂而發(fa)生(sheng)(sheng)(sheng)污(wu)染。3. 解凍(dong)后(hou)未(wei)開封前若發(fa)現(xian)有(you)(you)(you)絮狀沉淀(dian)，屬正常(chang)現(xian)象，主要是(shi)(shi)(shi)(shi)(shi)解凍(dong)后(hou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)纖維蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)及(ji)(ji)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)變(bian)性(xing)(xing)(xing)造(zao)成(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，不(bu)(bu)(bu)(bu)影(ying)響使(shi)(shi)用(yong)(yong)(yong)。可(ke)(ke)(ke)(ke)(ke)以(yi)通(tong)過(guo)(guo)(guo)3500rpm離(li)心(xin)5分(fen)(fen)鐘去除(chu)(chu)(chu)，但(dan)不(bu)(bu)(bu)(bu)宜(yi)過(guo)(guo)(guo)濾去除(chu)(chu)(chu)，因為(wei)(wei)(wei)(wei)絮狀物(wu)(wu)(wu)(wu)可(ke)(ke)(ke)(ke)(ke)能(neng)阻塞濾膜。 4. 本(ben)產(chan)品未(wei)經滅活(huo)(huo)處(chu)(chu)理(li)(li)(li)，使(shi)(shi)用(yong)(yong)(yong)時(shi)(shi)(shi)請視實(shi)驗情況(kuang)進(jin)(jin)行(xing)處(chu)(chu)理(li)(li)(li)，滅活(huo)(huo)條(tiao)件(jian)為(wei)(wei)(wei)(wei)56℃，30min。除(chu)(chu)(chu)非必(bi)(bi)須，一(yi)(yi)般不(bu)(bu)(bu)(bu)建(jian)議對(dui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)行(xing)熱(re)處(chu)(chu)理(li)(li)(li)，因為(wei)(wei)(wei)(wei)熱(re)處(chu)(chu)理(li)(li)(li)會(hui)造(zao)成(cheng)(cheng)(cheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)沉淀(dian)物(wu)(wu)(wu)(wu)顯著增多。 牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)通(tong)過(guo)(guo)(guo)無(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)手術(shu)剖(pou)腹(fu)后(hou)取(qu)(qu)胎，穿刺心(xin)臟采血(xue)(xue)(xue)(xue)(xue)(xue)(xue)制(zhi)成(cheng)(cheng)(cheng)。牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)所(suo)含(han)(han)(han)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)對(dui)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)有(you)(you)(you)害的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)分(fen)(fen)（抗體(ti)(ti)(ti)(ti)(ti)等(deng)）更少，故質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)更高(gao)。本(ben)產(chan)品以(yi)健康胎牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)為(wei)(wei)(wei)(wei)原(yuan)料(liao)加(jia)(jia)工而成(cheng)(cheng)(cheng)，不(bu)(bu)(bu)(bu)添(tian)加(jia)(jia)任何外(wai)(wai)(wai)源(yuan)因子(zi)，不(bu)(bu)(bu)(bu)含(han)(han)(han)激(ji)素(su)(su)等(deng)。經0.65μm、0.22 μm微孔濾膜預過(guo)(guo)(guo)濾，3次(ci)0.1μm微孔濾膜精過(guo)(guo)(guo)濾后(hou)，獲得成(cheng)(cheng)(cheng)品胎牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)。本(ben)產(chan)品無(wu)(wu)支原(yuan)體(ti)(ti)(ti)(ti)(ti)、無(wu)(wu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)、無(wu)(wu)噬菌(jun)(jun)(jun)(jun)(jun)體(ti)(ti)(ti)(ti)(ti)、BVDV病(bing)(bing)毒(du)(du)(du)(du)等(deng)污(wu)染，內(nei)毒(du)(du)(du)(du)素(su)(su)水平極(ji)低(di)。
標(biao)準(zhun)大(da)(da)(da)(da)牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)（細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)專(zhuan)用(yong)(yong)(yong)） 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)(shi)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)礎(chu)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)中(zhong)(zhong)(zhong)極為(wei)(wei)(wei)重要(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)和(he)(he)(he)(he)(he)粘附因(yin)子(zi)、激素(su)(su)(su)(su)(su)、脂(zhi)質(zhi)(zhi)(zhi)和(he)(he)(he)(he)(he)礦物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)來(lai)源。此外，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)還可(ke)調(diao)(diao)節細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)膜(mo)通透性(xing)(xing)，并(bing)可(ke)作(zuo)(zuo)(zuo)(zuo)為(wei)(wei)(wei)向細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)內運送脂(zhi)質(zhi)(zhi)(zhi)、酶、微量(liang)(liang)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)素(su)(su)(su)(su)(su)和(he)(he)(he)(he)(he)微量(liang)(liang)元素(su)(su)(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)載體(ti)(ti)(ti)。牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)用(yong)(yong)(yong)量(liang)(liang)天然(ran)(ran)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)，含(han)(han)有(you)(you)豐(feng)富(fu)的(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)必須的(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)成(cheng)(cheng)(cheng)(cheng)份，具(ju)有(you)(you)重要(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)功(gong)(gong)能(neng)(neng)(neng)。牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)分(fen)(fen)為(wei)(wei)(wei)小(xiao)(xiao)牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)、新牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)、胎(tai)牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)。 產(chan)(chan)品(pin)簡介 牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)(yi)種(zhong)(zhong)(zhong)(zhong)性(xing)(xing)狀(zhuang)、外觀(guan) 淺黃色澄清(qing)(qing)(qing)(qing)(qing)(qing)(qing)、無(wu)溶(rong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)、無(wu)異物(wu)(wu)(wu)(wu)(wu)稍(shao)粘稠液(ye)(ye)體(ti)(ti)(ti)。胎(tai)牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)應(ying)(ying)取自(zi)(zi)剖(pou)腹產(chan)(chan)的(de)(de)(de)(de)(de)(de)(de)胎(tai)牛(niu)(niu)(niu)(niu)；新牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)取自(zi)(zi)出(chu)生(sheng)(sheng)(sheng)(sheng)(sheng)24小(xiao)(xiao)時(shi)(shi)之(zhi)(zhi)內的(de)(de)(de)(de)(de)(de)(de)新生(sheng)(sheng)(sheng)(sheng)(sheng)牛(niu)(niu)(niu)(niu)；小(xiao)(xiao)牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)取自(zi)(zi)出(chu)生(sheng)(sheng)(sheng)(sheng)(sheng)10－30天的(de)(de)(de)(de)(de)(de)(de)小(xiao)(xiao)牛(niu)(niu)(niu)(niu)。1、性(xing)(xing)狀(zhuang)、外觀(guan) 淺黃色澄清(qing)(qing)(qing)(qing)(qing)(qing)(qing)、無(wu)溶(rong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)、無(wu)異物(wu)(wu)(wu)(wu)(wu)稍(shao)粘稠液(ye)(ye)體(ti)(ti)(ti)。2、蛋(dan)(dan)白(bai)質(zhi)(zhi)(zhi)含(han)(han)量(liang)(liang) 3.5%～5.0%（ w/v）3、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)紅蛋(dan)(dan)白(bai)含(han)(han)量(liang)(liang) ≤ 0.02%（ w/v）4、無(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)檢(jian)驗(yan) 陰(yin)(yin)(yin)性(xing)(xing)5、支(zhi)原(yuan)(yuan)體(ti)(ti)(ti)檢(jian)驗(yan) 陰(yin)(yin)(yin)性(xing)(xing)6、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)內毒(du)(du)素(su)(su)(su)(su)(su) ≤5（EU/ml）7、牛(niu)(niu)(niu)(niu)腹瀉病毒(du)(du) 陰(yin)(yin)(yin)性(xing)(xing)8、大(da)(da)(da)(da)腸桿菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)噬菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)體(ti)(ti)(ti) 陰(yin)(yin)(yin)性(xing)(xing)9、支(zhi)持(chi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)增殖(Sp2/0-Ag14) 生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)曲線（接種(zhong)(zhong)(zhong)(zhong)濃(nong)度(du)(du)）＊大(da)(da)(da)(da)增殖濃(nong)度(du)(du)≥2.5×106 個(ge)/ml細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)倍增時(shi)(shi)間 ≤15h克隆率(lv) ≥85%細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang) 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)是(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)(yi)種(zhong)(zhong)(zhong)(zhong)用(yong)(yong)(yong)人(ren)工方(fang)法(fa)使(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)繁殖的(de)(de)(de)(de)(de)(de)(de)技(ji)術(shu)(shu)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)在(zai)(zai)(zai)(zai)自(zi)(zi)然(ran)(ran)界中(zhong)(zhong)(zhong)分(fen)(fen)布極廣，數(shu)量(liang)(liang)大(da)(da)(da)(da)，種(zhong)(zhong)(zhong)(zhong)類(lei)(lei)多(duo)，它可(ke)以(yi)(yi)(yi)造福人(ren)類(lei)(lei)，也(ye)可(ke)以(yi)(yi)(yi)成(cheng)(cheng)(cheng)(cheng)為(wei)(wei)(wei)致(zhi)病的(de)(de)(de)(de)(de)(de)(de)原(yuan)(yuan)因(yin)。大(da)(da)(da)(da)多(duo)數(shu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)可(ke)用(yong)(yong)(yong)人(ren)工方(fang)法(fa)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)，即(ji)(ji)將其(qi)(qi)接種(zhong)(zhong)(zhong)(zhong)于培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)上，使(shi)其(qi)(qi)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)繁殖。培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)出(chu)來(lai)的(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)用(yong)(yong)(yong)于研究、鑒(jian)定(ding)和(he)(he)(he)(he)(he)應(ying)(ying)用(yong)(yong)(yong)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)是(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)(yi)個(ge)復(fu)雜的(de)(de)(de)(de)(de)(de)(de)技(ji)術(shu)(shu)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)條(tiao)件(jian) 培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)應(ying)(ying)根(gen)據細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)類(lei)(lei)和(he)(he)(he)(he)(he)目的(de)(de)(de)(de)(de)(de)(de)等(deng)(deng)選擇培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)方(fang)法(fa)、培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)，制(zhi)(zhi)定(ding)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)條(tiao)件(jian)(溫度(du)(du)、pH值(zhi)、時(shi)(shi)間，對(dui)(dui)(dui)氧(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)需(xu)求與否等(deng)(deng))。一(yi)(yi)(yi)般(ban)操作(zuo)(zuo)(zuo)(zuo)步驟(zou)為(wei)(wei)(wei)先將標(biao)本(ben)接種(zhong)(zhong)(zhong)(zhong)于固(gu)體(ti)(ti)(ti)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)上，做分(fen)(fen)離培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。再(zai)進一(yi)(yi)(yi)步對(dui)(dui)(dui)所(suo)得單個(ge)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)落(luo)進行(xing)形態、生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)及(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)學反(fan)應(ying)(ying)鑒(jian)定(ding)。培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)常(chang)用(yong)(yong)(yong)牛(niu)(niu)(niu)(niu)肉湯(tang)、蛋(dan)(dan)白(bai)胨、氯化(hua)(hua)鈉、葡萄糖(tang)、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)等(deng)(deng)和(he)(he)(he)(he)(he)某些(xie)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)所(suo)需(xu)的(de)(de)(de)(de)(de)(de)(de)特(te)殊物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)配(pei)(pei)制(zhi)(zhi)成(cheng)(cheng)(cheng)(cheng)液(ye)(ye)體(ti)(ti)(ti)、半(ban)固(gu)體(ti)(ti)(ti)、固(gu)體(ti)(ti)(ti)等(deng)(deng)。一(yi)(yi)(yi)般(ban)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)可(ke)在(zai)(zai)(zai)(zai)有(you)(you)氧(yang)(yang)條(tiao)件(jian)下，37℃中(zhong)(zhong)(zhong)放18~24小(xiao)(xiao)時(shi)(shi)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)。厭氧(yang)(yang)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)則(ze)需(xu)在(zai)(zai)(zai)(zai)無(wu)氧(yang)(yang)環境中(zhong)(zhong)(zhong)放2~3天后(hou)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)。個(ge)別(bie)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)如(ru)結(jie)核菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)要(yao)(yao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)1個(ge)月(yue)之(zhi)(zhi)久。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)步驟(zou) 以(yi)(yi)(yi)光(guang)(guang)合(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)方(fang)法(fa)為(wei)(wei)(wei)例。光(guang)(guang)合(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)方(fang)法(fa)，按次(ci)(ci)序分(fen)(fen)為(wei)(wei)(wei)容器、工具(ju)的(de)(de)(de)(de)(de)(de)(de)消(xiao)(xiao)(xiao)毒(du)(du)，培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)制(zhi)(zhi)備(bei)，接種(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)管(guan)(guan)理(li)(li)四個(ge)步驟(zou)。(一(yi)(yi)(yi))容器、工具(ju)的(de)(de)(de)(de)(de)(de)(de)消(xiao)(xiao)(xiao)毒(du)(du)參考、此處(chu)(chu)從(cong)略。(二(er)(er))培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)制(zhi)(zhi)備(bei)1.培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)用(yong)(yong)(yong)水(shui)(shui)(shui)(shui)如(ru)果(guo)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)光(guang)(guang)合(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)是(shi)(shi)(shi)(shi)(shi)(shi)淡水(shui)(shui)(shui)(shui)種(zhong)(zhong)(zhong)(zhong)，菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)可(ke)用(yong)(yong)(yong)蒸(zheng)餾(liu)水(shui)(shui)(shui)(shui)，生(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)可(ke)用(yong)(yong)(yong)消(xiao)(xiao)(xiao)毒(du)(du)的(de)(de)(de)(de)(de)(de)(de)自(zi)(zi)來(lai)水(shui)(shui)(shui)(shui)(或井水(shui)(shui)(shui)(shui))配(pei)(pei)制(zhi)(zhi)。如(ru)果(guo)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)光(guang)(guang)合(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)是(shi)(shi)(shi)(shi)(shi)(shi)海水(shui)(shui)(shui)(shui)種(zhong)(zhong)(zhong)(zhong)，則(ze)用(yong)(yong)(yong)天然(ran)(ran)海水(shui)(shui)(shui)(shui)配(pei)(pei)制(zhi)(zhi)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)，注意在(zai)(zai)(zai)(zai)海水(shui)(shui)(shui)(shui)中(zhong)(zhong)(zhong)加(jia)入(ru)磷元素(su)(su)(su)(su)(su)時(shi)(shi)，不(bu)(bu)(bu)(bu)(bu)能(neng)(neng)(neng)用(yong)(yong)(yong)磷酸氫二(er)(er)鉀(jia)(jia)，應(ying)(ying)用(yong)(yong)(yong)磷酸二(er)(er)氫鉀(jia)(jia)，不(bu)(bu)(bu)(bu)(bu)然(ran)(ran)會(hui)產(chan)(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)大(da)(da)(da)(da)量(liang)(liang)沉(chen)(chen)淀。2.滅菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)和(he)(he)(he)(he)(he)消(xiao)(xiao)(xiao)毒(du)(du)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)用(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)應(ying)(ying)連同培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)容器用(yong)(yong)(yong)高(gao)壓蒸(zheng)氣(qi)滅菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)鍋滅菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)。小(xiao)(xiao)型(xing)生(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)性(xing)(xing)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)可(ke)把(ba)(ba)(ba)配(pei)(pei)好的(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)液(ye)(ye)用(yong)(yong)(yong)普通鋁鍋或大(da)(da)(da)(da)型(xing)三(san)角燒瓶(ping)煮沸消(xiao)(xiao)(xiao)毒(du)(du)。大(da)(da)(da)(da)型(xing)生(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)性(xing)(xing)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)則(ze)把(ba)(ba)(ba)經(jing)(jing)沉(chen)(chen)淀砂濾(lv)后(hou)的(de)(de)(de)(de)(de)(de)(de)水(shui)(shui)(shui)(shui)用(yong)(yong)(yong)漂白(bai)粉(fen)(或漂白(bai)液(ye)(ye))消(xiao)(xiao)(xiao)毒(du)(du)后(hou)使(shi)用(yong)(yong)(yong)。3.培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)配(pei)(pei)制(zhi)(zhi)根(gen)據所(suo)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)種(zhong)(zhong)(zhong)(zhong)類(lei)(lei)的(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)需(xu)要(yao)(yao)選擇合(he)適的(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)配(pei)(pei)方(fang)。按培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)配(pei)(pei)方(fang)把(ba)(ba)(ba)所(suo)需(xu)物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)稱量(liang)(liang)，逐一(yi)(yi)(yi)溶(rong)解(jie)，混合(he)，配(pei)(pei)成(cheng)(cheng)(cheng)(cheng)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)。也(ye)可(ke)先配(pei)(pei)成(cheng)(cheng)(cheng)(cheng)母液(ye)(ye)，使(shi)用(yong)(yong)(yong)時(shi)(shi)按比例加(jia)入(ru)一(yi)(yi)(yi)定(ding)的(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)即(ji)(ji)可(ke)。(三(san))接種(zhong)(zhong)(zhong)(zhong)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)配(pei)(pei)好后(hou)，應(ying)(ying)立(li)即(ji)(ji)進行(xing)接種(zhong)(zhong)(zhong)(zhong)。光(guang)(guang)合(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)生(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)性(xing)(xing)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)按種(zhong)(zhong)(zhong)(zhong)量(liang)(liang)比較高(gao)，一(yi)(yi)(yi)般(ban)為(wei)(wei)(wei)20%~50%，即(ji)(ji)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)(zhong)母液(ye)(ye)量(liang)(liang)和(he)(he)(he)(he)(he)新配(pei)(pei)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)液(ye)(ye)雖之(zhi)(zhi)比為(wei)(wei)(wei)1∶4~1∶1，不(bu)(bu)(bu)(bu)(bu)應(ying)(ying)低(di)(di)于20%，尤其(qi)(qi)是(shi)(shi)(shi)(shi)(shi)(shi)微氣(qi)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)，接種(zhong)(zhong)(zhong)(zhong)量(liang)(liang)更應(ying)(ying)高(gao)些(xie)，否則(ze)光(guang)(guang)合(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)在(zai)(zai)(zai)(zai)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)液(ye)(ye)中(zhong)(zhong)(zhong)很(hen)難占＊＊優(you)勢，影(ying)響(xiang)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)＊終產(chan)(chan)量(liang)(liang)和(he)(he)(he)(he)(he)質(zhi)(zhi)(zhi)量(liang)(liang)。(四)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)管(guan)(guan)理(li)(li)光(guang)(guang)合(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)的(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)過(guo)程中(zhong)(zhong)(zhong)，管(guan)(guan)理(li)(li)工作(zuo)(zuo)(zuo)(zuo)包括(kuo)日常(chang)管(guan)(guan)理(li)(li)操作(zuo)(zuo)(zuo)(zuo)和(he)(he)(he)(he)(he)測試，生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)情(qing)(qing)況(kuang)(kuang)的(de)(de)(de)(de)(de)(de)(de)觀(guan)察、檢(jian)查以(yi)(yi)(yi)及(ji)出(chu)現問(wen)題的(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)析處(chu)(chu)理(li)(li)等(deng)(deng)三(san)個(ge)方(fang)面。標(biao)準(zhun)大(da)(da)(da)(da)牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)（細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)專(zhuan)用(yong)(yong)(yong)）產(chan)(chan)品(pin)功(gong)(gong)能(neng)(neng)(neng) 牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)用(yong)(yong)(yong)量(liang)(liang)＊大(da)(da)(da)(da)的(de)(de)(de)(de)(de)(de)(de)天然(ran)(ran)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)，含(han)(han)有(you)(you)豐(feng)富(fu)的(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)必須的(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)成(cheng)(cheng)(cheng)(cheng)份，常(chang)用(yong)(yong)(yong)于動物(wu)(wu)(wu)(wu)(wu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)體(ti)(ti)(ti)外培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)，具(ju)有(you)(you)極為(wei)(wei)(wei)重要(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)功(gong)(gong)能(neng)(neng)(neng)。1.提(ti)(ti)供(gong)(gong)(gong)對(dui)(dui)(dui)維(wei)持(chi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)指(zhi)數(shu)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)的(de)(de)(de)(de)(de)(de)(de)激素(su)(su)(su)(su)(su)，基(ji)(ji)(ji)(ji)(ji)(ji)(ji)礎(chu)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)中(zhong)(zhong)(zhong)沒有(you)(you)或量(liang)(liang)很(hen)少的(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)，以(yi)(yi)(yi)及(ji)主要(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)低(di)(di)分(fen)(fen)子(zi)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)。2. 提(ti)(ti)供(gong)(gong)(gong)結(jie)合(he)蛋(dan)(dan)白(bai)，能(neng)(neng)(neng)識(shi)別(bie)維(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)素(su)(su)(su)(su)(su)、脂(zhi)類(lei)(lei)、金(jin)屬和(he)(he)(he)(he)(he)其(qi)(qi)他激素(su)(su)(su)(su)(su)等(deng)(deng)，能(neng)(neng)(neng)結(jie)合(he)或調(diao)(diao)變它們所(suo)結(jie)合(he)的(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)活(huo)力。3.有(you)(you)些(xie)情(qing)(qing)況(kuang)(kuang)下結(jie)合(he)蛋(dan)(dan)白(bai)質(zhi)(zhi)(zhi)能(neng)(neng)(neng)與有(you)(you)毒(du)(du)金(jin)屬和(he)(he)(he)(he)(he)熱原(yuan)(yuan)質(zhi)(zhi)(zhi)結(jie)合(he)，起到(dao)解(jie)毒(du)(du)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)。4.是(shi)(shi)(shi)(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)貼壁(bi)、鋪(pu)展在(zai)(zai)(zai)(zai)塑料培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)質(zhi)(zhi)(zhi)上所(suo)需(xu)因(yin)子(zi)來(lai)源。5.起酸堿度(du)(du)緩(huan)沖(chong)液(ye)(ye)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)。6.提(ti)(ti)供(gong)(gong)(gong)蛋(dan)(dan)白(bai)酶抑制(zhi)(zhi)劑，使(shi)在(zai)(zai)(zai)(zai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)傳(chuan)代(dai)時(shi)(shi)使(shi)剩余胰(yi)蛋(dan)(dan)白(bai)酶失(shi)活(huo)，保(bao)護細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)不(bu)(bu)(bu)(bu)(bu)受(shou)傷害。適用(yong)(yong)(yong)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)多(duo)種(zhong)(zhong)(zhong)(zhong)腫瘤原(yuan)(yuan)代(dai)和(he)(he)(he)(he)(he)293、293T、CHO、SF9、Vero、Hela細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)系，各種(zhong)(zhong)(zhong)(zhong)腫瘤細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)等(deng)(deng)保(bao)存條(tiao)件(jian)1. -20℃，五年2. 4oC保(bao)存通常(chang)不(bu)(bu)(bu)(bu)(bu)宜(yi)(yi)超過(guo)1個(ge)月(yue)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)主要(yao)(yao)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)(shi)由(you)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)去除纖維(wei)蛋(dan)(dan)白(bai)而(er)形成(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)種(zhong)(zhong)(zhong)(zhong)很(hen)復(fu)雜的(de)(de)(de)(de)(de)(de)(de)混合(he)物(wu)(wu)(wu)(wu)(wu)，其(qi)(qi)組成(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)份雖大(da)(da)(da)(da)部分(fen)(fen)已知，但(dan)還有(you)(you)一(yi)(yi)(yi)部分(fen)(fen)尚不(bu)(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)組成(cheng)(cheng)(cheng)(cheng)及(ji)含(han)(han)量(liang)(liang)常(chang)隨供(gong)(gong)(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)別(bie)、年齡、生(sheng)(sheng)(sheng)(sheng)(sheng)理(li)(li)條(tiao)件(jian)和(he)(he)(he)(he)(he)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)條(tiao)件(jian)不(bu)(bu)(bu)(bu)(bu)同而(er)異。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)含(han)(han)有(you)(you)各種(zhong)(zhong)(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)蛋(dan)(dan)白(bai)、多(duo)肽、脂(zhi)肪(fang)、碳水(shui)(shui)(shui)(shui)化(hua)(hua)合(he)物(wu)(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)子(zi)、激素(su)(su)(su)(su)(su)、無(wu)機(ji)物(wu)(wu)(wu)(wu)(wu)等(deng)(deng)，這些(xie)物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)對(dui)(dui)(dui)促進細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)或抑制(zhi)(zhi)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)活(huo)性(xing)(xing)是(shi)(shi)(shi)(shi)(shi)(shi)達到(dao)生(sheng)(sheng)(sheng)(sheng)(sheng)理(li)(li)平(ping)衡(heng)的(de)(de)(de)(de)(de)(de)(de)。 牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)用(yong)(yong)(yong)量(liang)(liang)＊大(da)(da)(da)(da)的(de)(de)(de)(de)(de)(de)(de)天然(ran)(ran)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)，含(han)(han)有(you)(you)豐(feng)富(fu)的(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)必須的(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)成(cheng)(cheng)(cheng)(cheng)份，常(chang)用(yong)(yong)(yong)于動物(wu)(wu)(wu)(wu)(wu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)體(ti)(ti)(ti)外培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)，具(ju)有(you)(you)極為(wei)(wei)(wei)重要(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)功(gong)(gong)能(neng)(neng)(neng)。1.提(ti)(ti)供(gong)(gong)(gong)對(dui)(dui)(dui)維(wei)持(chi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)指(zhi)數(shu)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)的(de)(de)(de)(de)(de)(de)(de)激素(su)(su)(su)(su)(su)，基(ji)(ji)(ji)(ji)(ji)(ji)(ji)礎(chu)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)中(zhong)(zhong)(zhong)沒有(you)(you)或量(liang)(liang)很(hen)少的(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)，以(yi)(yi)(yi)及(ji)主要(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)低(di)(di)分(fen)(fen)子(zi)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)。2. 提(ti)(ti)供(gong)(gong)(gong)結(jie)合(he)蛋(dan)(dan)白(bai)，能(neng)(neng)(neng)識(shi)別(bie)維(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)素(su)(su)(su)(su)(su)、脂(zhi)類(lei)(lei)、金(jin)屬和(he)(he)(he)(he)(he)其(qi)(qi)他激素(su)(su)(su)(su)(su)等(deng)(deng)，能(neng)(neng)(neng)結(jie)合(he)或調(diao)(diao)變它們所(suo)結(jie)合(he)的(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)活(huo)力。3.有(you)(you)些(xie)情(qing)(qing)況(kuang)(kuang)下結(jie)合(he)蛋(dan)(dan)白(bai)質(zhi)(zhi)(zhi)能(neng)(neng)(neng)與有(you)(you)毒(du)(du)金(jin)屬和(he)(he)(he)(he)(he)熱原(yuan)(yuan)質(zhi)(zhi)(zhi)結(jie)合(he)，起到(dao)解(jie)毒(du)(du)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)。4.是(shi)(shi)(shi)(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)貼壁(bi)、鋪(pu)展在(zai)(zai)(zai)(zai)塑料培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)(ji)質(zhi)(zhi)(zhi)上所(suo)需(xu)因(yin)子(zi)來(lai)源。5.起酸堿度(du)(du)緩(huan)沖(chong)液(ye)(ye)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)。6.提(ti)(ti)供(gong)(gong)(gong)蛋(dan)(dan)白(bai)酶抑制(zhi)(zhi)劑，使(shi)在(zai)(zai)(zai)(zai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)傳(chuan)代(dai)時(shi)(shi)使(shi)剩余胰(yi)蛋(dan)(dan)白(bai)酶失(shi)活(huo)，保(bao)護細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)不(bu)(bu)(bu)(bu)(bu)受(shou)傷害。注意事項1. 融(rong)化(hua)(hua)方(fang)法(fa)：建(jian)議將血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)-20℃冰箱取出(chu)后(hou)，于2-8℃解(jie)凍過(guo)夜(ye)，并(bing)在(zai)(zai)(zai)(zai)此過(guo)程中(zhong)(zhong)(zhong)不(bu)(bu)(bu)(bu)(bu)時(shi)(shi)輕輕搖(yao)動使(shi)之(zhi)(zhi)溶(rong)解(jie)均(jun)勻。2. 不(bu)(bu)(bu)(bu)(bu)宜(yi)(yi) 4℃甚至更高(gao)溫度(du)(du)長(chang)(chang)期保(bao)存，切忌反(fan)復(fu)凍融(rong)，小(xiao)(xiao)量(liang)(liang)使(shi)用(yong)(yong)(yong)時(shi)(shi)建(jian)議分(fen)(fen)裝(zhuang)凍存。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)結(jie)冰時(shi)(shi)體(ti)(ti)(ti)積會(hui)增加(jia)約10%，因(yin)此在(zai)(zai)(zai)(zai)分(fen)(fen)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)須預留一(yi)(yi)(yi)定(ding)體(ti)(ti)(ti)積，否則(ze)易導致(zhi)分(fen)(fen)裝(zhuang)瓶(ping)凍裂而(er)發生(sheng)(sheng)(sheng)(sheng)(sheng)污染(ran)。3. 解(jie)凍后(hou)未開封(feng)前若發現有(you)(you)絮(xu)狀(zhuang)沉(chen)(chen)淀，屬正常(chang)現象(xiang)，主要(yao)(yao)是(shi)(shi)(shi)(shi)(shi)(shi)解(jie)凍后(hou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)纖維(wei)蛋(dan)(dan)白(bai)及(ji)脂(zhi)蛋(dan)(dan)白(bai)變性(xing)(xing)造成(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)，不(bu)(bu)(bu)(bu)(bu)影(ying)響(xiang)使(shi)用(yong)(yong)(yong)。可(ke)以(yi)(yi)(yi)通過(guo)3500rpm離心(xin)(xin)5分(fen)(fen)鐘去除，但(dan)不(bu)(bu)(bu)(bu)(bu)宜(yi)(yi)過(guo)濾(lv)去除，因(yin)為(wei)(wei)(wei)絮(xu)狀(zhuang)物(wu)(wu)(wu)(wu)(wu)可(ke)能(neng)(neng)(neng)阻塞濾(lv)膜(mo)。 4. 本(ben)產(chan)(chan)品(pin)未經(jing)(jing)滅活(huo)處(chu)(chu)理(li)(li)，使(shi)用(yong)(yong)(yong)時(shi)(shi)請視實驗(yan)情(qing)(qing)況(kuang)(kuang)進行(xing)處(chu)(chu)理(li)(li)，滅活(huo)條(tiao)件(jian)為(wei)(wei)(wei)56℃，30min。除非必須，一(yi)(yi)(yi)般(ban)不(bu)(bu)(bu)(bu)(bu)建(jian)議對(dui)(dui)(dui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)進行(xing)熱處(chu)(chu)理(li)(li)，因(yin)為(wei)(wei)(wei)熱處(chu)(chu)理(li)(li)會(hui)造成(cheng)(cheng)(cheng)(cheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)沉(chen)(chen)淀物(wu)(wu)(wu)(wu)(wu)顯著增多(duo)。 牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)(shi)通過(guo)無(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)手術(shu)(shu)剖(pou)腹后(hou)取胎(tai)，穿刺心(xin)(xin)臟采血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)制(zhi)(zhi)成(cheng)(cheng)(cheng)(cheng)。牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)所(suo)含(han)(han)的(de)(de)(de)(de)(de)(de)(de)對(dui)(dui)(dui)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)有(you)(you)害的(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)（抗體(ti)(ti)(ti)等(deng)(deng)）更少，故質(zhi)(zhi)(zhi)量(liang)(liang)更高(gao)。本(ben)產(chan)(chan)品(pin)以(yi)(yi)(yi)健康胎(tai)牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)為(wei)(wei)(wei)原(yuan)(yuan)料加(jia)工而(er)成(cheng)(cheng)(cheng)(cheng)，不(bu)(bu)(bu)(bu)(bu)添(tian)加(jia)任何外源因(yin)子(zi)，不(bu)(bu)(bu)(bu)(bu)含(han)(han)激素(su)(su)(su)(su)(su)等(deng)(deng)。經(jing)(jing)0.65μm、0.22 μm微孔濾(lv)膜(mo)預過(guo)濾(lv)，3次(ci)(ci)0.1μm微孔濾(lv)膜(mo)精(jing)過(guo)濾(lv)后(hou)，獲得成(cheng)(cheng)(cheng)(cheng)品(pin)胎(tai)牛(niu)(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)。本(ben)產(chan)(chan)品(pin)無(wu)支(zhi)原(yuan)(yuan)體(ti)(ti)(ti)、無(wu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)、無(wu)噬菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)體(ti)(ti)(ti)、BVDV病毒(du)(du)等(deng)(deng)污染(ran)，內毒(du)(du)素(su)(su)(su)(su)(su)水(shui)(shui)(shui)(shui)平(ping)極低(di)(di)。
標準新(xin)生(sheng)牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)（細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)專(zhuan)用(yong)(yong)(yong)(yong)） 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)基(ji)(ji)礎(chu)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)中(zhong)(zhong)極(ji)(ji)為(wei)(wei)重(zhong)要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)長(chang)(chang)(chang)和(he)(he)(he)(he)粘附因(yin)子(zi)、激(ji)(ji)素(su)(su)(su)、脂質(zhi)(zhi)(zhi)(zhi)和(he)(he)(he)(he)礦物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)來源(yuan)。此(ci)外(wai)(wai)(wai)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)還可(ke)(ke)調節(jie)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)膜通(tong)透(tou)性(xing)(xing)，并可(ke)(ke)作(zuo)(zuo)(zuo)(zuo)為(wei)(wei)向細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)內運送脂質(zhi)(zhi)(zhi)(zhi)、酶、微(wei)(wei)量(liang)(liang)(liang)(liang)(liang)營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)素(su)(su)(su)和(he)(he)(he)(he)微(wei)(wei)量(liang)(liang)(liang)(liang)(liang)元素(su)(su)(su)的(de)(de)(de)(de)(de)(de)(de)(de)載體(ti)(ti)(ti)(ti)(ti)(ti)。牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)用(yong)(yong)(yong)(yong)量(liang)(liang)(liang)(liang)(liang)天(tian)(tian)然(ran)(ran)(ran)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)，含(han)(han)(han)有(you)(you)(you)(you)豐富的(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)長(chang)(chang)(chang)必(bi)(bi)須(xu)的(de)(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)成(cheng)(cheng)(cheng)(cheng)份，具有(you)(you)(you)(you)重(zhong)要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)功(gong)能(neng)(neng)(neng)(neng)。牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)分(fen)(fen)(fen)(fen)為(wei)(wei)小(xiao)牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)、新(xin)牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)、胎(tai)(tai)(tai)牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)。 產(chan)品(pin)簡介 胎(tai)(tai)(tai)牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)一(yi)(yi)(yi)(yi)種(zhong)性(xing)(xing)狀(zhuang)(zhuang)、外(wai)(wai)(wai)觀 淺黃色(se)澄清(qing)(qing)(qing)(qing)(qing)、無(wu)溶(rong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)、無(wu)異(yi)物(wu)(wu)(wu)(wu)(wu)稍粘稠液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)。胎(tai)(tai)(tai)牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)應(ying)取(qu)自(zi)(zi)剖腹(fu)產(chan)的(de)(de)(de)(de)(de)(de)(de)(de)胎(tai)(tai)(tai)牛(niu)(niu)(niu)；新(xin)牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)取(qu)自(zi)(zi)出(chu)(chu)生(sheng)24小(xiao)時(shi)(shi)(shi)(shi)之(zhi)內的(de)(de)(de)(de)(de)(de)(de)(de)新(xin)生(sheng)牛(niu)(niu)(niu)；小(xiao)牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)取(qu)自(zi)(zi)出(chu)(chu)生(sheng)10－30天(tian)(tian)的(de)(de)(de)(de)(de)(de)(de)(de)小(xiao)牛(niu)(niu)(niu)。 顯(xian)然(ran)(ran)(ran)，胎(tai)(tai)(tai)牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)品(pin)質(zhi)(zhi)(zhi)(zhi)＊高的(de)(de)(de)(de)(de)(de)(de)(de)，因(yin)為(wei)(wei)胎(tai)(tai)(tai)牛(niu)(niu)(niu)還未(wei)(wei)接觸外(wai)(wai)(wai)界，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)所(suo)含(han)(han)(han)的(de)(de)(de)(de)(de)(de)(de)(de)抗體(ti)(ti)(ti)(ti)(ti)(ti)、補體(ti)(ti)(ti)(ti)(ti)(ti)等(deng)(deng)(deng)對細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)有(you)(you)(you)(you)害(hai)的(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)＊少(shao)。1、性(xing)(xing)狀(zhuang)(zhuang)、外(wai)(wai)(wai)觀 淺黃色(se)澄清(qing)(qing)(qing)(qing)(qing)、無(wu)溶(rong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)、無(wu)異(yi)物(wu)(wu)(wu)(wu)(wu)稍粘稠液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)。2、蛋(dan)(dan)(dan)(dan)白(bai)質(zhi)(zhi)(zhi)(zhi)含(han)(han)(han)量(liang)(liang)(liang)(liang)(liang) 3.5%～5.0%（ w/v）3、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)紅(hong)蛋(dan)(dan)(dan)(dan)白(bai)含(han)(han)(han)量(liang)(liang)(liang)(liang)(liang) ≤ 0.02%（ w/v）4、無(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)檢(jian)驗 陰(yin)(yin)性(xing)(xing)5、支原(yuan)體(ti)(ti)(ti)(ti)(ti)(ti)檢(jian)驗 陰(yin)(yin)性(xing)(xing)6、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)內毒(du)素(su)(su)(su) ≤5（EU/ml）7、牛(niu)(niu)(niu)腹(fu)瀉(xie)病(bing)毒(du) 陰(yin)(yin)性(xing)(xing)8、大(da)(da)腸(chang)桿菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)噬菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)體(ti)(ti)(ti)(ti)(ti)(ti) 陰(yin)(yin)性(xing)(xing)9、支持細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)增(zeng)(zeng)殖(Sp2/0-Ag14) 生(sheng)長(chang)(chang)(chang)曲線（接種(zhong)濃度）＊大(da)(da)增(zeng)(zeng)殖濃度≥2.5×106 個(ge)/ml細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)倍增(zeng)(zeng)時(shi)(shi)(shi)(shi)間 ≤15h克隆率 ≥85%細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang) 細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)是(shi)(shi)(shi)一(yi)(yi)(yi)(yi)種(zhong)用(yong)(yong)(yong)(yong)人(ren)工(gong)方(fang)(fang)(fang)法(fa)使(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)生(sheng)長(chang)(chang)(chang)繁殖的(de)(de)(de)(de)(de)(de)(de)(de)技(ji)術。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)在(zai)(zai)自(zi)(zi)然(ran)(ran)(ran)界中(zhong)(zhong)分(fen)(fen)(fen)(fen)布極(ji)(ji)廣(guang)，數(shu)量(liang)(liang)(liang)(liang)(liang)大(da)(da)，種(zhong)類(lei)(lei)(lei)多(duo)，它(ta)可(ke)(ke)以造(zao)福人(ren)類(lei)(lei)(lei)，也(ye)可(ke)(ke)以成(cheng)(cheng)(cheng)(cheng)為(wei)(wei)致(zhi)病(bing)的(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)因(yin)。大(da)(da)多(duo)數(shu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)可(ke)(ke)用(yong)(yong)(yong)(yong)人(ren)工(gong)方(fang)(fang)(fang)法(fa)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)，即(ji)將其(qi)(qi)(qi)接種(zhong)于(yu)(yu)(yu)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)上(shang)(shang)，使(shi)(shi)(shi)其(qi)(qi)(qi)生(sheng)長(chang)(chang)(chang)繁殖。培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)出(chu)(chu)來的(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)研究(jiu)、鑒定(ding)和(he)(he)(he)(he)應(ying)用(yong)(yong)(yong)(yong)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)是(shi)(shi)(shi)一(yi)(yi)(yi)(yi)個(ge)復雜(za)(za)的(de)(de)(de)(de)(de)(de)(de)(de)技(ji)術。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)條(tiao)件(jian)(jian)(jian) 培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)應(ying)根據(ju)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)類(lei)(lei)(lei)和(he)(he)(he)(he)目的(de)(de)(de)(de)(de)(de)(de)(de)等(deng)(deng)(deng)選(xuan)擇(ze)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)方(fang)(fang)(fang)法(fa)、培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)，制(zhi)(zhi)定(ding)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)條(tiao)件(jian)(jian)(jian)(溫(wen)(wen)度、pH值、時(shi)(shi)(shi)(shi)間，對氧(yang)的(de)(de)(de)(de)(de)(de)(de)(de)需(xu)(xu)求與否(fou)(fou)等(deng)(deng)(deng))。一(yi)(yi)(yi)(yi)般(ban)(ban)操作(zuo)(zuo)(zuo)(zuo)步(bu)驟為(wei)(wei)先將標本(ben)接種(zhong)于(yu)(yu)(yu)固體(ti)(ti)(ti)(ti)(ti)(ti)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)上(shang)(shang)，做分(fen)(fen)(fen)(fen)離(li)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。再進(jin)(jin)一(yi)(yi)(yi)(yi)步(bu)對所(suo)得單個(ge)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)落進(jin)(jin)行形(xing)態、生(sheng)化(hua)(hua)及(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)學反應(ying)鑒定(ding)。培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)常用(yong)(yong)(yong)(yong)牛(niu)(niu)(niu)肉湯、蛋(dan)(dan)(dan)(dan)白(bai)胨、氯化(hua)(hua)鈉、葡萄糖、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)等(deng)(deng)(deng)和(he)(he)(he)(he)某些(xie)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)所(suo)需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)特(te)殊(shu)物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)配(pei)制(zhi)(zhi)成(cheng)(cheng)(cheng)(cheng)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)、半固體(ti)(ti)(ti)(ti)(ti)(ti)、固體(ti)(ti)(ti)(ti)(ti)(ti)等(deng)(deng)(deng)。一(yi)(yi)(yi)(yi)般(ban)(ban)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)可(ke)(ke)在(zai)(zai)有(you)(you)(you)(you)氧(yang)條(tiao)件(jian)(jian)(jian)下，37℃中(zhong)(zhong)放18~24小(xiao)時(shi)(shi)(shi)(shi)生(sheng)長(chang)(chang)(chang)。厭(yan)氧(yang)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)則(ze)(ze)需(xu)(xu)在(zai)(zai)無(wu)氧(yang)環境(jing)中(zhong)(zhong)放2~3天(tian)(tian)后(hou)(hou)(hou)(hou)(hou)生(sheng)長(chang)(chang)(chang)。個(ge)別細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)如(ru)結(jie)核菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)要(yao)(yao)(yao)(yao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)1個(ge)月之(zhi)久(jiu)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)步(bu)驟 以光合(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)方(fang)(fang)(fang)法(fa)為(wei)(wei)例(li)。光合(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)(fang)法(fa)，按次序(xu)分(fen)(fen)(fen)(fen)為(wei)(wei)容器(qi)(qi)、工(gong)具的(de)(de)(de)(de)(de)(de)(de)(de)消(xiao)毒(du)，培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)(zhi)備，接種(zhong)和(he)(he)(he)(he)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)管(guan)(guan)理(li)(li)四個(ge)步(bu)驟。(一(yi)(yi)(yi)(yi))容器(qi)(qi)、工(gong)具的(de)(de)(de)(de)(de)(de)(de)(de)消(xiao)毒(du)參考、此(ci)處(chu)(chu)從略。(二)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)(zhi)備1.培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)用(yong)(yong)(yong)(yong)水(shui)如(ru)果培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)光合(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)是(shi)(shi)(shi)淡水(shui)種(zhong)，菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)可(ke)(ke)用(yong)(yong)(yong)(yong)蒸餾水(shui)，生(sheng)產(chan)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)可(ke)(ke)用(yong)(yong)(yong)(yong)消(xiao)毒(du)的(de)(de)(de)(de)(de)(de)(de)(de)自(zi)(zi)來水(shui)(或(huo)井水(shui))配(pei)制(zhi)(zhi)。如(ru)果培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)光合(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)是(shi)(shi)(shi)海水(shui)種(zhong)，則(ze)(ze)用(yong)(yong)(yong)(yong)天(tian)(tian)然(ran)(ran)(ran)海水(shui)配(pei)制(zhi)(zhi)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)，注意在(zai)(zai)海水(shui)中(zhong)(zhong)加(jia)入(ru)磷元素(su)(su)(su)時(shi)(shi)(shi)(shi)，不(bu)(bu)能(neng)(neng)(neng)(neng)用(yong)(yong)(yong)(yong)磷酸氫二鉀，應(ying)用(yong)(yong)(yong)(yong)磷酸二氫鉀，不(bu)(bu)然(ran)(ran)(ran)會(hui)產(chan)生(sheng)大(da)(da)量(liang)(liang)(liang)(liang)(liang)沉淀。2.滅(mie)(mie)(mie)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)和(he)(he)(he)(he)消(xiao)毒(du)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)用(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)應(ying)連同培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)容器(qi)(qi)用(yong)(yong)(yong)(yong)高壓蒸氣滅(mie)(mie)(mie)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)鍋(guo)滅(mie)(mie)(mie)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)。小(xiao)型(xing)(xing)生(sheng)產(chan)性(xing)(xing)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)可(ke)(ke)把配(pei)好(hao)的(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)液(ye)(ye)(ye)(ye)(ye)用(yong)(yong)(yong)(yong)普通(tong)鋁鍋(guo)或(huo)大(da)(da)型(xing)(xing)三(san)角燒瓶(ping)煮沸(fei)消(xiao)毒(du)。大(da)(da)型(xing)(xing)生(sheng)產(chan)性(xing)(xing)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)則(ze)(ze)把經(jing)沉淀砂濾后(hou)(hou)(hou)(hou)(hou)的(de)(de)(de)(de)(de)(de)(de)(de)水(shui)用(yong)(yong)(yong)(yong)漂(piao)白(bai)粉(或(huo)漂(piao)白(bai)液(ye)(ye)(ye)(ye)(ye))消(xiao)毒(du)后(hou)(hou)(hou)(hou)(hou)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)。3.培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)配(pei)制(zhi)(zhi)根據(ju)所(suo)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)種(zhong)類(lei)(lei)(lei)的(de)(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)需(xu)(xu)要(yao)(yao)(yao)(yao)選(xuan)擇(ze)合(he)(he)適(shi)的(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)配(pei)方(fang)(fang)(fang)。按培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)配(pei)方(fang)(fang)(fang)把所(suo)需(xu)(xu)物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)稱量(liang)(liang)(liang)(liang)(liang)，逐一(yi)(yi)(yi)(yi)溶(rong)解(jie)(jie)(jie)(jie)，混(hun)合(he)(he)，配(pei)成(cheng)(cheng)(cheng)(cheng)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)。也(ye)可(ke)(ke)先配(pei)成(cheng)(cheng)(cheng)(cheng)母液(ye)(ye)(ye)(ye)(ye)，使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)時(shi)(shi)(shi)(shi)按比(bi)例(li)加(jia)入(ru)一(yi)(yi)(yi)(yi)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)(liang)(liang)即(ji)可(ke)(ke)。(三(san))接種(zhong)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)配(pei)好(hao)后(hou)(hou)(hou)(hou)(hou)，應(ying)立即(ji)進(jin)(jin)行接種(zhong)。光合(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)生(sheng)產(chan)性(xing)(xing)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)按種(zhong)量(liang)(liang)(liang)(liang)(liang)比(bi)較高，一(yi)(yi)(yi)(yi)般(ban)(ban)為(wei)(wei)20%~50%，即(ji)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)母液(ye)(ye)(ye)(ye)(ye)量(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)新(xin)配(pei)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)液(ye)(ye)(ye)(ye)(ye)雖之(zhi)比(bi)為(wei)(wei)1∶4~1∶1，不(bu)(bu)應(ying)低(di)于(yu)(yu)(yu)20%，尤其(qi)(qi)(qi)是(shi)(shi)(shi)微(wei)(wei)氣培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)，接種(zhong)量(liang)(liang)(liang)(liang)(liang)更應(ying)高些(xie)，否(fou)(fou)則(ze)(ze)光合(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)在(zai)(zai)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)液(ye)(ye)(ye)(ye)(ye)中(zhong)(zhong)很(hen)(hen)(hen)難占(zhan)＊＊優勢，影響培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)＊終產(chan)量(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)。(四)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)管(guan)(guan)理(li)(li)光合(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)的(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)過(guo)程(cheng)中(zhong)(zhong)，管(guan)(guan)理(li)(li)工(gong)作(zuo)(zuo)(zuo)(zuo)包括(kuo)日常管(guan)(guan)理(li)(li)操作(zuo)(zuo)(zuo)(zuo)和(he)(he)(he)(he)測試，生(sheng)長(chang)(chang)(chang)情況(kuang)的(de)(de)(de)(de)(de)(de)(de)(de)觀察、檢(jian)查以及(ji)出(chu)(chu)現(xian)問題的(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)(fen)(fen)析處(chu)(chu)理(li)(li)等(deng)(deng)(deng)三(san)個(ge)方(fang)(fang)(fang)面。產(chan)品(pin)功(gong)能(neng)(neng)(neng)(neng) 牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)用(yong)(yong)(yong)(yong)量(liang)(liang)(liang)(liang)(liang)＊大(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)天(tian)(tian)然(ran)(ran)(ran)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)，含(han)(han)(han)有(you)(you)(you)(you)豐富的(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)長(chang)(chang)(chang)必(bi)(bi)須(xu)的(de)(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)成(cheng)(cheng)(cheng)(cheng)份，常用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)動(dong)物(wu)(wu)(wu)(wu)(wu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)體(ti)(ti)(ti)(ti)(ti)(ti)外(wai)(wai)(wai)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)，具有(you)(you)(you)(you)極(ji)(ji)為(wei)(wei)重(zhong)要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)功(gong)能(neng)(neng)(neng)(neng)。1.提(ti)供(gong)對維(wei)(wei)(wei)持細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)指數(shu)生(sheng)長(chang)(chang)(chang)的(de)(de)(de)(de)(de)(de)(de)(de)激(ji)(ji)素(su)(su)(su)，基(ji)(ji)礎(chu)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)中(zhong)(zhong)沒有(you)(you)(you)(you)或(huo)量(liang)(liang)(liang)(liang)(liang)很(hen)(hen)(hen)少(shao)的(de)(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)，以及(ji)主(zhu)要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)低(di)分(fen)(fen)(fen)(fen)子(zi)營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)。2. 提(ti)供(gong)結(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)白(bai)，能(neng)(neng)(neng)(neng)識(shi)別維(wei)(wei)(wei)生(sheng)素(su)(su)(su)、脂類(lei)(lei)(lei)、金(jin)屬(shu)(shu)和(he)(he)(he)(he)其(qi)(qi)(qi)他激(ji)(ji)素(su)(su)(su)等(deng)(deng)(deng)，能(neng)(neng)(neng)(neng)結(jie)合(he)(he)或(huo)調變(bian)它(ta)們所(suo)結(jie)合(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)活(huo)力。3.有(you)(you)(you)(you)些(xie)情況(kuang)下結(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)白(bai)質(zhi)(zhi)(zhi)(zhi)能(neng)(neng)(neng)(neng)與有(you)(you)(you)(you)毒(du)金(jin)屬(shu)(shu)和(he)(he)(he)(he)熱(re)(re)原(yuan)質(zhi)(zhi)(zhi)(zhi)結(jie)合(he)(he)，起(qi)(qi)到解(jie)(jie)(jie)(jie)毒(du)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)。4.是(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)貼壁(bi)、鋪展(zhan)在(zai)(zai)塑料培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)質(zhi)(zhi)(zhi)(zhi)上(shang)(shang)所(suo)需(xu)(xu)因(yin)子(zi)來源(yuan)。5.起(qi)(qi)酸堿(jian)度緩(huan)沖液(ye)(ye)(ye)(ye)(ye)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)。6.提(ti)供(gong)蛋(dan)(dan)(dan)(dan)白(bai)酶抑(yi)制(zhi)(zhi)劑，使(shi)(shi)(shi)在(zai)(zai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)傳代時(shi)(shi)(shi)(shi)使(shi)(shi)(shi)剩余胰蛋(dan)(dan)(dan)(dan)白(bai)酶失(shi)活(huo)，保(bao)護細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)不(bu)(bu)受(shou)傷(shang)害(hai)。適(shi)用(yong)(yong)(yong)(yong)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)多(duo)種(zhong)腫(zhong)瘤(liu)原(yuan)代和(he)(he)(he)(he)293、293T、CHO、SF9、Vero、Hela細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)系，各(ge)種(zhong)腫(zhong)瘤(liu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)等(deng)(deng)(deng)保(bao)存(cun)(cun)條(tiao)件(jian)(jian)(jian)1. -20℃，五年(nian)(nian)2. 4oC保(bao)存(cun)(cun)通(tong)常不(bu)(bu)宜超過(guo)1個(ge)月血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要(yao)(yao)(yao)(yao)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)由(you)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿去(qu)除(chu)纖維(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)白(bai)而形(xing)成(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)種(zhong)很(hen)(hen)(hen)復雜(za)(za)的(de)(de)(de)(de)(de)(de)(de)(de)混(hun)合(he)(he)物(wu)(wu)(wu)(wu)(wu)，其(qi)(qi)(qi)組成(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)份雖大(da)(da)部分(fen)(fen)(fen)(fen)已知，但(dan)還有(you)(you)(you)(you)一(yi)(yi)(yi)(yi)部分(fen)(fen)(fen)(fen)尚不(bu)(bu)清(qing)(qing)(qing)(qing)(qing)楚(chu)，且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)組成(cheng)(cheng)(cheng)(cheng)及(ji)含(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)常隨供(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)別、年(nian)(nian)齡、生(sheng)理(li)(li)條(tiao)件(jian)(jian)(jian)和(he)(he)(he)(he)營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)條(tiao)件(jian)(jian)(jian)不(bu)(bu)同而異(yi)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)含(han)(han)(han)有(you)(you)(you)(you)各(ge)種(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿蛋(dan)(dan)(dan)(dan)白(bai)、多(duo)肽、脂肪、碳水(shui)化(hua)(hua)合(he)(he)物(wu)(wu)(wu)(wu)(wu)、生(sheng)長(chang)(chang)(chang)因(yin)子(zi)、激(ji)(ji)素(su)(su)(su)、無(wu)機物(wu)(wu)(wu)(wu)(wu)等(deng)(deng)(deng)，這些(xie)物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)對促進(jin)(jin)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)長(chang)(chang)(chang)或(huo)抑(yi)制(zhi)(zhi)生(sheng)長(chang)(chang)(chang)活(huo)性(xing)(xing)是(shi)(shi)(shi)達到生(sheng)理(li)(li)平衡(heng)的(de)(de)(de)(de)(de)(de)(de)(de)。 牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)用(yong)(yong)(yong)(yong)量(liang)(liang)(liang)(liang)(liang)＊大(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)天(tian)(tian)然(ran)(ran)(ran)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)，含(han)(han)(han)有(you)(you)(you)(you)豐富的(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)長(chang)(chang)(chang)必(bi)(bi)須(xu)的(de)(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)成(cheng)(cheng)(cheng)(cheng)份，常用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)動(dong)物(wu)(wu)(wu)(wu)(wu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)體(ti)(ti)(ti)(ti)(ti)(ti)外(wai)(wai)(wai)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)，具有(you)(you)(you)(you)極(ji)(ji)為(wei)(wei)重(zhong)要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)功(gong)能(neng)(neng)(neng)(neng)。1.提(ti)供(gong)對維(wei)(wei)(wei)持細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)指數(shu)生(sheng)長(chang)(chang)(chang)的(de)(de)(de)(de)(de)(de)(de)(de)激(ji)(ji)素(su)(su)(su)，基(ji)(ji)礎(chu)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)中(zhong)(zhong)沒有(you)(you)(you)(you)或(huo)量(liang)(liang)(liang)(liang)(liang)很(hen)(hen)(hen)少(shao)的(de)(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)，以及(ji)主(zhu)要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)低(di)分(fen)(fen)(fen)(fen)子(zi)營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)。2. 提(ti)供(gong)結(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)白(bai)，能(neng)(neng)(neng)(neng)識(shi)別維(wei)(wei)(wei)生(sheng)素(su)(su)(su)、脂類(lei)(lei)(lei)、金(jin)屬(shu)(shu)和(he)(he)(he)(he)其(qi)(qi)(qi)他激(ji)(ji)素(su)(su)(su)等(deng)(deng)(deng)，能(neng)(neng)(neng)(neng)結(jie)合(he)(he)或(huo)調變(bian)它(ta)們所(suo)結(jie)合(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)活(huo)力。3.有(you)(you)(you)(you)些(xie)情況(kuang)下結(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)白(bai)質(zhi)(zhi)(zhi)(zhi)能(neng)(neng)(neng)(neng)與有(you)(you)(you)(you)毒(du)金(jin)屬(shu)(shu)和(he)(he)(he)(he)熱(re)(re)原(yuan)質(zhi)(zhi)(zhi)(zhi)結(jie)合(he)(he)，起(qi)(qi)到解(jie)(jie)(jie)(jie)毒(du)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)。4.是(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)貼壁(bi)、鋪展(zhan)在(zai)(zai)塑料培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)質(zhi)(zhi)(zhi)(zhi)上(shang)(shang)所(suo)需(xu)(xu)因(yin)子(zi)來源(yuan)。5.起(qi)(qi)酸堿(jian)度緩(huan)沖液(ye)(ye)(ye)(ye)(ye)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)。6.提(ti)供(gong)蛋(dan)(dan)(dan)(dan)白(bai)酶抑(yi)制(zhi)(zhi)劑，使(shi)(shi)(shi)在(zai)(zai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)傳代時(shi)(shi)(shi)(shi)使(shi)(shi)(shi)剩余胰蛋(dan)(dan)(dan)(dan)白(bai)酶失(shi)活(huo)，保(bao)護細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)不(bu)(bu)受(shou)傷(shang)害(hai)。注意事項(xiang)1. 融化(hua)(hua)方(fang)(fang)(fang)法(fa)：建議將血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)從-20℃冰(bing)(bing)箱取(qu)出(chu)(chu)后(hou)(hou)(hou)(hou)(hou)，于(yu)(yu)(yu)2-8℃解(jie)(jie)(jie)(jie)凍(dong)過(guo)夜，并在(zai)(zai)此(ci)過(guo)程(cheng)中(zhong)(zhong)不(bu)(bu)時(shi)(shi)(shi)(shi)輕輕搖動(dong)使(shi)(shi)(shi)之(zhi)溶(rong)解(jie)(jie)(jie)(jie)均勻。2. 不(bu)(bu)宜 4℃甚至更高溫(wen)(wen)度長(chang)(chang)(chang)期保(bao)存(cun)(cun)，切忌反復凍(dong)融，小(xiao)量(liang)(liang)(liang)(liang)(liang)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)時(shi)(shi)(shi)(shi)建議分(fen)(fen)(fen)(fen)裝凍(dong)存(cun)(cun)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)結(jie)冰(bing)(bing)時(shi)(shi)(shi)(shi)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)會(hui)增(zeng)(zeng)加(jia)約10%，因(yin)此(ci)在(zai)(zai)分(fen)(fen)(fen)(fen)裝血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)時(shi)(shi)(shi)(shi)須(xu)預(yu)留一(yi)(yi)(yi)(yi)定(ding)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)，否(fou)(fou)則(ze)(ze)易(yi)導(dao)致(zhi)分(fen)(fen)(fen)(fen)裝瓶(ping)凍(dong)裂而發(fa)(fa)生(sheng)污染(ran)。3. 解(jie)(jie)(jie)(jie)凍(dong)后(hou)(hou)(hou)(hou)(hou)未(wei)(wei)開封前若發(fa)(fa)現(xian)有(you)(you)(you)(you)絮(xu)狀(zhuang)(zhuang)沉淀，屬(shu)(shu)正常現(xian)象，主(zhu)要(yao)(yao)(yao)(yao)是(shi)(shi)(shi)解(jie)(jie)(jie)(jie)凍(dong)后(hou)(hou)(hou)(hou)(hou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)纖維(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)白(bai)及(ji)脂蛋(dan)(dan)(dan)(dan)白(bai)變(bian)性(xing)(xing)造(zao)成(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)，不(bu)(bu)影響使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)。可(ke)(ke)以通(tong)過(guo)3500rpm離(li)心5分(fen)(fen)(fen)(fen)鐘去(qu)除(chu)，但(dan)不(bu)(bu)宜過(guo)濾去(qu)除(chu)，因(yin)為(wei)(wei)絮(xu)狀(zhuang)(zhuang)物(wu)(wu)(wu)(wu)(wu)可(ke)(ke)能(neng)(neng)(neng)(neng)阻塞濾膜。 4. 本(ben)產(chan)品(pin)未(wei)(wei)經(jing)滅(mie)(mie)(mie)活(huo)處(chu)(chu)理(li)(li)，使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)時(shi)(shi)(shi)(shi)請視實驗情況(kuang)進(jin)(jin)行處(chu)(chu)理(li)(li)，滅(mie)(mie)(mie)活(huo)條(tiao)件(jian)(jian)(jian)為(wei)(wei)56℃，30min。除(chu)非(fei)必(bi)(bi)須(xu)，一(yi)(yi)(yi)(yi)般(ban)(ban)不(bu)(bu)建議對血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)進(jin)(jin)行熱(re)(re)處(chu)(chu)理(li)(li)，因(yin)為(wei)(wei)熱(re)(re)處(chu)(chu)理(li)(li)會(hui)造(zao)成(cheng)(cheng)(cheng)(cheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)沉淀物(wu)(wu)(wu)(wu)(wu)顯(xian)著增(zeng)(zeng)多(duo)。 胎(tai)(tai)(tai)牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)通(tong)過(guo)無(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)手術剖腹(fu)后(hou)(hou)(hou)(hou)(hou)取(qu)胎(tai)(tai)(tai)，穿刺(ci)心臟采血(xue)(xue)(xue)(xue)(xue)(xue)(xue)制(zhi)(zhi)成(cheng)(cheng)(cheng)(cheng)。胎(tai)(tai)(tai)牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)所(suo)含(han)(han)(han)的(de)(de)(de)(de)(de)(de)(de)(de)對培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)有(you)(you)(you)(you)害(hai)的(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)（抗體(ti)(ti)(ti)(ti)(ti)(ti)等(deng)(deng)(deng)）更少(shao)，故質(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)更高。本(ben)產(chan)品(pin)以健康(kang)胎(tai)(tai)(tai)牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)為(wei)(wei)原(yuan)料加(jia)工(gong)而成(cheng)(cheng)(cheng)(cheng)，不(bu)(bu)添加(jia)任(ren)何外(wai)(wai)(wai)源(yuan)因(yin)子(zi)，不(bu)(bu)含(han)(han)(han)激(ji)(ji)素(su)(su)(su)等(deng)(deng)(deng)。經(jing)0.65μm、0.22 μm微(wei)(wei)孔濾膜預(yu)過(guo)濾，3次0.1μm微(wei)(wei)孔濾膜精過(guo)濾后(hou)(hou)(hou)(hou)(hou)，獲(huo)得成(cheng)(cheng)(cheng)(cheng)品(pin)胎(tai)(tai)(tai)牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)。本(ben)產(chan)品(pin)無(wu)支原(yuan)體(ti)(ti)(ti)(ti)(ti)(ti)、無(wu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)、無(wu)噬菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)體(ti)(ti)(ti)(ti)(ti)(ti)、BVDV病(bing)毒(du)等(deng)(deng)(deng)污染(ran)，內毒(du)素(su)(su)(su)水(shui)平極(ji)(ji)低(di)。
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