驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)（去(qu)(qu)(qu)(qu)紅細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)、無(wu)(wu)(wu)菌(jun)(jun)(jun)過(guo)(guo)(guo)(guo)濾(lv)）使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)方法根據(ju)具(ju)體(ti)實驗來使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)。商(shang)品(pin)簡介 驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)通(tong)過(guo)(guo)(guo)(guo)無(wu)(wu)(wu)菌(jun)(jun)(jun)采(cai)集(ji)驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)，凝(ning)固離心(xin)后(hou)獲得驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。注(zhu)意(yi)事項(xiang)1、注(zhu)意(yi)無(wu)(wu)(wu)菌(jun)(jun)(jun)操作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)，避免反(fan)復(fu)凍(dong)(dong)(dong)融。2、反(fan)復(fu)凍(dong)(dong)(dong)融可(ke)(ke)(ke)(ke)(ke)能(neng)(neng)產生(sheng)(sheng)(sheng)(sheng)沉淀，可(ke)(ke)(ke)(ke)(ke)以(yi)通(tong)過(guo)(guo)(guo)(guo)無(wu)(wu)(wu)菌(jun)(jun)(jun)條(tiao)(tiao)(tiao)件下(xia)，3500 rpm離心(xin)10 min除(chu)去(qu)(qu)(qu)(qu)沉淀。 驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)采(cai)用(yong)(yong)(yong)(yong)(yong)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)年健康驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)為原(yuan)(yuan)(yuan)料加工(gong)而(er)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)，經(jing)無(wu)(wu)(wu)菌(jun)(jun)(jun)采(cai)集(ji)、分(fen)(fen)(fen)(fen)(fen)(fen)(fen)離、微(wei)孔過(guo)(guo)(guo)(guo)濾(lv)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)(zhuang)而(er)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)。無(wu)(wu)(wu)支原(yuan)(yuan)(yuan)體(ti)、無(wu)(wu)(wu)噬菌(jun)(jun)(jun)體(ti)，內(nei)毒素(su)(su)含(han)(han)量(liang)(liang)(liang)低于(yu)50EU/ml。 驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)由(you)(you)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)去(qu)(qu)(qu)(qu)除(chu)纖(xian)(xian)維(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)而(er)形成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)種(zhong)(zhong)很(hen)復(fu)雜(za)(za)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混(hun)合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)，其(qi)組(zu)(zu)(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)(fen)雖(sui)大(da)(da)部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)已(yi)知(zhi)，但還(huan)有(you)(you)(you)一(yi)(yi)(yi)(yi)(yi)部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)尚不(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組(zu)(zu)(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)及(ji)(ji)(ji)含(han)(han)量(liang)(liang)(liang)常(chang)(chang)(chang)隨供(gong)(gong)(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)(xing)別(bie)、年齡(ling)、生(sheng)(sheng)(sheng)(sheng)理(li)條(tiao)(tiao)(tiao)件和(he)(he)(he)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)條(tiao)(tiao)(tiao)件不(bu)(bu)(bu)同而(er)異。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)中(zhong)(zhong)不(bu)(bu)(bu)含(han)(han)纖(xian)(xian)維(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠(jiao)狀液(ye)(ye)(ye)體(ti),具(ju)有(you)(you)(you)維(wei)(wei)(wei)(wei)(wei)持(chi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正(zheng)常(chang)(chang)(chang)粘度、酸(suan)(suan)堿(jian)(jian)度、滲透(tou)壓等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。它(ta)主(zhu)(zhu)要(yao)(yao)(yao)由(you)(you)水和(he)(he)(he)各(ge)(ge)(ge)(ge)(ge)種(zhong)(zhong)化(hua)(hua)(hua)(hua)學(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)組(zu)(zu)(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng),這(zhe)些(xie)(xie)(xie)(xie)化(hua)(hua)(hua)(hua)學(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)包(bao)(bao)括白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、α1、α2、β、γ-球蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai),甘(gan)油(you)三(san)酯(zhi)(zhi),總膽(dan)固醇,谷丙轉(zhuan)氨(an)(an)酶(mei)(mei)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)含(han)(han)有(you)(you)(you)各(ge)(ge)(ge)(ge)(ge)種(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、多肽(tai)(tai)(tai)(tai)、脂肪、碳水化(hua)(hua)(hua)(hua)合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)(yin)(yin)子(zi)(zi)(zi)(zi)、激(ji)(ji)素(su)(su)、無(wu)(wu)(wu)機(ji)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)，這(zhe)些(xie)(xie)(xie)(xie)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)對(dui)(dui)(dui)(dui)(dui)促進(jin)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)或(huo)抑(yi)制(zhi)(zhi)生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)活(huo)性(xing)(xing)(xing)是(shi)(shi)(shi)達到(dao)生(sheng)(sheng)(sheng)(sheng)理(li)平衡的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。概述 驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)指血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)凝(ning)固后(hou)，在血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)中(zhong)(zhong)除(chu)去(qu)(qu)(qu)(qu)纖(xian)(xian)維(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)離出的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)淡(dan)黃(huang)色透(tou)明液(ye)(ye)(ye)體(ti)或(huo)指纖(xian)(xian)維(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)已(yi)被除(chu)去(qu)(qu)(qu)(qu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)，用(yong)(yong)(yong)(yong)(yong)于(yu)科研(yan)(yan)(yan)(yan)、診斷試劑(ji)生(sheng)(sheng)(sheng)(sheng)產等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)。驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)提(ti)(ti)(ti)(ti)(ti)供(gong)(gong)(gong)基(ji)本(ben)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)、提(ti)(ti)(ti)(ti)(ti)供(gong)(gong)(gong)激(ji)(ji)素(su)(su)和(he)(he)(he)各(ge)(ge)(ge)(ge)(ge)種(zhong)(zhong)生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)(yin)(yin)子(zi)(zi)(zi)(zi)、提(ti)(ti)(ti)(ti)(ti)供(gong)(gong)(gong)結(jie)(jie)(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、提(ti)(ti)(ti)(ti)(ti)供(gong)(gong)(gong)促接(jie)觸。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)應(ying)保(bao)(bao)(bao)存在-5℃至-20℃，若存放(fang)于(yu)4C時勿超過(guo)(guo)(guo)(guo)一(yi)(yi)(yi)(yi)(yi)個月，若一(yi)(yi)(yi)(yi)(yi)次無(wu)(wu)(wu)法用(yong)(yong)(yong)(yong)(yong)完一(yi)(yi)(yi)(yi)(yi)瓶，將無(wu)(wu)(wu)菌(jun)(jun)(jun)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)至恰當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)滅菌(jun)(jun)(jun)容(rong)器內(nei)，再放(fang)回冷凍(dong)(dong)(dong)。背景 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，指血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)凝(ning)固后(hou)，在血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)中(zhong)(zhong)除(chu)去(qu)(qu)(qu)(qu)纖(xian)(xian)維(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)離出的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)淡(dan)黃(huang)色透(tou)明液(ye)(ye)(ye)體(ti)或(huo)指纖(xian)(xian)維(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)已(yi)被除(chu)去(qu)(qu)(qu)(qu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)。其(qi)主(zhu)(zhu)要(yao)(yao)(yao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)提(ti)(ti)(ti)(ti)(ti)供(gong)(gong)(gong)基(ji)本(ben)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)、提(ti)(ti)(ti)(ti)(ti)供(gong)(gong)(gong)激(ji)(ji)素(su)(su)和(he)(he)(he)各(ge)(ge)(ge)(ge)(ge)種(zhong)(zhong)生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)(yin)(yin)子(zi)(zi)(zi)(zi)、提(ti)(ti)(ti)(ti)(ti)供(gong)(gong)(gong)結(jie)(jie)(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、提(ti)(ti)(ti)(ti)(ti)供(gong)(gong)(gong)促接(jie)觸和(he)(he)(he)伸(shen)展因(yin)(yin)(yin)子(zi)(zi)(zi)(zi)使(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)貼(tie)(tie)壁(bi)(bi)免受(shou)機(ji)械(xie)損(sun)傷(shang)(shang)、對(dui)(dui)(dui)(dui)(dui)培(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)起(qi)(qi)到(dao)某些(xie)(xie)(xie)(xie)保(bao)(bao)(bao)護(hu)(hu)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)性(xing)(xing)(xing)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)由(you)(you)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)去(qu)(qu)(qu)(qu)除(chu)纖(xian)(xian)維(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)而(er)形成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)種(zhong)(zhong)很(hen)復(fu)雜(za)(za)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混(hun)合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)，其(qi)組(zu)(zu)(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)(fen)雖(sui)大(da)(da)部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)已(yi)知(zhi)，但還(huan)有(you)(you)(you)一(yi)(yi)(yi)(yi)(yi)部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)尚不(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組(zu)(zu)(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)及(ji)(ji)(ji)含(han)(han)量(liang)(liang)(liang)常(chang)(chang)(chang)隨供(gong)(gong)(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)(xing)別(bie)、年齡(ling)、生(sheng)(sheng)(sheng)(sheng)理(li)條(tiao)(tiao)(tiao)件和(he)(he)(he)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)條(tiao)(tiao)(tiao)件不(bu)(bu)(bu)同而(er)異。作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)中(zhong)(zhong)不(bu)(bu)(bu)含(han)(han)纖(xian)(xian)維(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠(jiao)狀液(ye)(ye)(ye)體(ti),具(ju)有(you)(you)(you)維(wei)(wei)(wei)(wei)(wei)持(chi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正(zheng)常(chang)(chang)(chang)粘度、酸(suan)(suan)堿(jian)(jian)度、滲透(tou)壓等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。它(ta)主(zhu)(zhu)要(yao)(yao)(yao)由(you)(you)水和(he)(he)(he)各(ge)(ge)(ge)(ge)(ge)種(zhong)(zhong)化(hua)(hua)(hua)(hua)學(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)組(zu)(zu)(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng),這(zhe)些(xie)(xie)(xie)(xie)化(hua)(hua)(hua)(hua)學(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)包(bao)(bao)括白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、α1、Chemicalbookα2、β、γ-球蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai),甘(gan)油(you)三(san)酯(zhi)(zhi),總膽(dan)固醇,谷丙轉(zhuan)氨(an)(an)酶(mei)(mei)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)含(han)(han)有(you)(you)(you)各(ge)(ge)(ge)(ge)(ge)種(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、多肽(tai)(tai)(tai)(tai)、脂肪、碳水化(hua)(hua)(hua)(hua)合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)(yin)(yin)子(zi)(zi)(zi)(zi)、激(ji)(ji)素(su)(su)、無(wu)(wu)(wu)機(ji)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)，這(zhe)些(xie)(xie)(xie)(xie)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)對(dui)(dui)(dui)(dui)(dui)促進(jin)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)或(huo)抑(yi)制(zhi)(zhi)生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)活(huo)性(xing)(xing)(xing)是(shi)(shi)(shi)達到(dao)生(sheng)(sheng)(sheng)(sheng)理(li)平衡的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。儲(chu)存條(tiao)(tiao)(tiao)件 需(xu)(xu)要(yao)(yao)(yao)長(chang)(chang)期(qi)保(bao)(bao)(bao)存的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必(bi)須(xu)(xu)儲(chu)存于(yu)-20℃-70℃低溫冰箱(xiang)中(zhong)(zhong).4℃冰箱(xiang)中(zhong)(zhong)保(bao)(bao)(bao)存時間(jian)切(qie)勿超過(guo)(guo)(guo)(guo)1個月.由(you)(you)于(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結(jie)(jie)(jie)冰時體(ti)積會增(zeng)(zeng)(zeng)(zeng)加約10%,因(yin)(yin)(yin)此,血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在凍(dong)(dong)(dong)入低溫冰箱(xiang)前,必(bi)須(xu)(xu)預留(liu)一(yi)(yi)(yi)(yi)(yi)定(ding)體(ti)積空間(jian),否則易發生(sheng)(sheng)(sheng)(sheng)污染(ran)或(huo)玻(bo)璃瓶凍(dong)(dong)(dong)裂.應(ying)用(yong)(yong)(yong)(yong)(yong) 驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)培(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)用(yong)(yong)(yong)(yong)(yong)量(liang)(liang)(liang)很(hen)大(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)天然(ran)培(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)，含(han)(han)有(you)(you)(you)豐富的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)必(bi)須(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)(fen)，常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)于(yu)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)體(ti)外培(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)，具(ju)有(you)(you)(you)極為重(zhong)要(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)功能(neng)(neng)。提(ti)(ti)(ti)(ti)(ti)供(gong)(gong)(gong)對(dui)(dui)(dui)(dui)(dui)維(wei)(wei)(wei)(wei)(wei)持(chi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)指數(shu)(shu)(shu)生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)激(ji)(ji)素(su)(su)，基(ji)礎培(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)中(zhong)(zhong)沒有(you)(you)(you)或(huo)量(liang)(liang)(liang)很(hen)少的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)，以(yi)及(ji)(ji)(ji)主(zhu)(zhu)要(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低分(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)(zi)(zi)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)。提(ti)(ti)(ti)(ti)(ti)供(gong)(gong)(gong)結(jie)(jie)(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)，能(neng)(neng)識別(bie)維(wei)(wei)(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)素(su)(su)、脂類(lei)、金屬和(he)(he)(he)其(qi)他激(ji)(ji)素(su)(su)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)，能(neng)(neng)結(jie)(jie)(jie)合(he)(he)或(huo)調變它(ta)們所(suo)結(jie)(jie)(jie)合(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)活(huo)力(li)。有(you)(you)(you)些(xie)(xie)(xie)(xie)情況下(xia)結(jie)(jie)(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)能(neng)(neng)與(yu)有(you)(you)(you)毒金屬和(he)(he)(he)熱原(yuan)(yuan)(yuan)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)結(jie)(jie)(jie)合(he)(he)，起(qi)(qi)到(dao)解毒作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。是(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)貼(tie)(tie)壁(bi)(bi)、鋪(pu)展在塑料培(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)上(shang)所(suo)需(xu)(xu)因(yin)(yin)(yin)子(zi)(zi)(zi)(zi)來源。起(qi)(qi)酸(suan)(suan)堿(jian)(jian)度緩沖液(ye)(ye)(ye)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。提(ti)(ti)(ti)(ti)(ti)供(gong)(gong)(gong)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)(mei)抑(yi)制(zhi)(zhi)劑(ji)，使(shi)(shi)(shi)在細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)傳代時使(shi)(shi)(shi)剩余胰(yi)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)(mei)失活(huo)，保(bao)(bao)(bao)護(hu)(hu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)不(bu)(bu)(bu)受(shou)傷(shang)(shang)害。驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)（去(qu)(qu)(qu)(qu)紅細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)、無(wu)(wu)(wu)菌(jun)(jun)(jun)過(guo)(guo)(guo)(guo)濾(lv)）主(zhu)(zhu)要(yao)(yao)(yao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)由(you)(you)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)去(qu)(qu)(qu)(qu)除(chu)纖(xian)(xian)維(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)而(er)形成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)種(zhong)(zhong)很(hen)復(fu)雜(za)(za)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混(hun)合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)，其(qi)組(zu)(zu)(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)(fen)雖(sui)大(da)(da)部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)已(yi)知(zhi)，但還(huan)有(you)(you)(you)一(yi)(yi)(yi)(yi)(yi)部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)尚不(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組(zu)(zu)(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)及(ji)(ji)(ji)含(han)(han)量(liang)(liang)(liang)常(chang)(chang)(chang)隨供(gong)(gong)(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)(xing)別(bie)、年齡(ling)、生(sheng)(sheng)(sheng)(sheng)理(li)條(tiao)(tiao)(tiao)件和(he)(he)(he)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)條(tiao)(tiao)(tiao)件不(bu)(bu)(bu)同而(er)異。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)中(zhong)(zhong)不(bu)(bu)(bu)含(han)(han)纖(xian)(xian)維(wei)(wei)(wei)(wei)(wei)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠(jiao)狀液(ye)(ye)(ye)體(ti),具(ju)有(you)(you)(you)維(wei)(wei)(wei)(wei)(wei)持(chi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正(zheng)常(chang)(chang)(chang)粘度、酸(suan)(suan)堿(jian)(jian)度、滲透(tou)壓等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。它(ta)主(zhu)(zhu)要(yao)(yao)(yao)由(you)(you)水和(he)(he)(he)各(ge)(ge)(ge)(ge)(ge)種(zhong)(zhong)化(hua)(hua)(hua)(hua)學(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)組(zu)(zu)(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)，這(zhe)些(xie)(xie)(xie)(xie)化(hua)(hua)(hua)(hua)學(xue)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)包(bao)(bao)括白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)，α1、α2、β、γ-球蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)，甘(gan)油(you)三(san)酯(zhi)(zhi)，總膽(dan)固醇，谷丙轉(zhuan)氨(an)(an)酶(mei)(mei)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)含(han)(han)有(you)(you)(you)各(ge)(ge)(ge)(ge)(ge)種(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)、多肽(tai)(tai)(tai)(tai)、脂肪、碳水化(hua)(hua)(hua)(hua)合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)(yin)(yin)子(zi)(zi)(zi)(zi)、激(ji)(ji)素(su)(su)、無(wu)(wu)(wu)機(ji)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)，這(zhe)些(xie)(xie)(xie)(xie)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)對(dui)(dui)(dui)(dui)(dui)促進(jin)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)或(huo)抑(yi)制(zhi)(zhi)生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)活(huo)性(xing)(xing)(xing)是(shi)(shi)(shi)達到(dao)生(sheng)(sheng)(sheng)(sheng)理(li)平衡的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。對(dui)(dui)(dui)(dui)(dui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)和(he)(he)(he)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)研(yan)(yan)(yan)(yan)究(jiu)雖(sui)有(you)(you)(you)很(hen)大(da)(da)進(jin)展，但仍存在一(yi)(yi)(yi)(yi)(yi)些(xie)(xie)(xie)(xie)問(wen)題(ti)。主(zhu)(zhu)要(yao)(yao)(yao)是(shi)(shi)(shi)：第一(yi)(yi)(yi)(yi)(yi)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)(fen)可(ke)(ke)(ke)(ke)(ke)能(neng)(neng)有(you)(you)(you)幾百種(zhong)(zhong)之多，對(dui)(dui)(dui)(dui)(dui)其(qi)準確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)(fen)、含(han)(han)量(liang)(liang)(liang)及(ji)(ji)(ji)其(qi)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)機(ji)制(zhi)(zhi)仍不(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，尤其(qi)是(shi)(shi)(shi)對(dui)(dui)(dui)(dui)(dui)其(qi)中(zhong)(zhong)一(yi)(yi)(yi)(yi)(yi)些(xie)(xie)(xie)(xie)多肽(tai)(tai)(tai)(tai)類(lei)生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)(yin)(yin)子(zi)(zi)(zi)(zi)、激(ji)(ji)素(su)(su)和(he)(he)(he)脂類(lei)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)尚未充分(fen)(fen)(fen)(fen)(fen)(fen)(fen)認識，這(zhe)給研(yan)(yan)(yan)(yan)究(jiu)工(gong)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)帶來許多困難(nan)；第二：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都(dou)是(shi)(shi)(shi)批量(liang)(liang)(liang)生(sheng)(sheng)(sheng)(sheng)產，各(ge)(ge)(ge)(ge)(ge)批量(liang)(liang)(liang)之間(jian)差異很(hen)大(da)(da)，而(er)且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)保(bao)(bao)(bao)存期(qi)至多一(yi)(yi)(yi)(yi)(yi)年，因(yin)(yin)(yin)此，要(yao)(yao)(yao)保(bao)(bao)(bao)證每批血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)相(xiang)(xiang)(xiang)似性(xing)(xing)(xing)極為困難(nan)，從而(er)使(shi)(shi)(shi)實驗的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)準化(hua)(hua)(hua)(hua)和(he)(he)(he)連續性(xing)(xing)(xing)受(shou)到(dao)限制(zhi)(zhi)；第三(san)：不(bu)(bu)(bu)能(neng)(neng)排除(chu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)含(han)(han)有(you)(you)(you)易變物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，這(zhe)被認為是(shi)(shi)(shi)“瓶中(zhong)(zhong)惡化(hua)(hua)(hua)(hua)”的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)(yuan)(yuan)因(yin)(yin)(yin)之一(yi)(yi)(yi)(yi)(yi)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)主(zhu)(zhu)要(yao)(yao)(yao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)●提(ti)(ti)(ti)(ti)(ti)供(gong)(gong)(gong)基(ji)本(ben)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)：氨(an)(an)基(ji)酸(suan)(suan)、維(wei)(wei)(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)素(su)(su)、無(wu)(wu)(wu)機(ji)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、脂類(lei)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)、核(he)酸(suan)(suan)衍生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)，是(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)必(bi)須(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。●提(ti)(ti)(ti)(ti)(ti)供(gong)(gong)(gong)激(ji)(ji)素(su)(su)和(he)(he)(he)各(ge)(ge)(ge)(ge)(ge)種(zhong)(zhong)生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)(yin)(yin)子(zi)(zi)(zi)(zi)：胰(yi)島素(su)(su)、腎(shen)上(shang)腺皮質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)激(ji)(ji)素(su)(su)（氫(qing)化(hua)(hua)(hua)(hua)可(ke)(ke)(ke)(ke)(ke)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)松、地塞米(mi)松）、類(lei)固醇激(ji)(ji)素(su)(su)（雌二醇、睪酮、孕酮）等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)。生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)(yin)(yin)子(zi)(zi)(zi)(zi)如成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)纖(xian)(xian)維(wei)(wei)(wei)(wei)(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)(yin)(yin)子(zi)(zi)(zi)(zi)、表(biao)皮生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)(yin)(yin)子(zi)(zi)(zi)(zi)、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)(xiao)板(ban)(ban)生(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)(yin)(yin)子(zi)(zi)(zi)(zi)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)。●提(ti)(ti)(ti)(ti)(ti)供(gong)(gong)(gong)結(jie)(jie)(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)：結(jie)(jie)(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)攜帶重(zhong)要(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低分(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)(zi)(zi)量(liang)(liang)(liang)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)，如白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜帶維(wei)(wei)(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)素(su)(su)、脂肪、以(yi)及(ji)(ji)(ji)激(ji)(ji)素(su)(su)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)，轉(zhuan)鐵(tie)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜帶鐵(tie)。結(jie)(jie)(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)在細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)代謝過(guo)(guo)(guo)(guo)程中(zhong)(zhong)起(qi)(qi)重(zhong)要(yao)(yao)(yao)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。●提(ti)(ti)(ti)(ti)(ti)供(gong)(gong)(gong)促接(jie)觸和(he)(he)(he)伸(shen)展因(yin)(yin)(yin)子(zi)(zi)(zi)(zi)使(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)貼(tie)(tie)壁(bi)(bi)免受(shou)機(ji)械(xie)損(sun)傷(shang)(shang)。●對(dui)(dui)(dui)(dui)(dui)培(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)起(qi)(qi)到(dao)某些(xie)(xie)(xie)(xie)保(bao)(bao)(bao)護(hu)(hu)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)：有(you)(you)(you)一(yi)(yi)(yi)(yi)(yi)些(xie)(xie)(xie)(xie)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，如內(nei)皮細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)、骨(gu)(gu)髓(sui)樣細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)可(ke)(ke)(ke)(ke)(ke)以(yi)釋放(fang)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)(mei)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)含(han)(han)有(you)(you)(you)抗蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶(mei)(mei)驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)用(yong)(yong)(yong)(yong)(yong)驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)于(yu)研(yan)(yan)(yan)(yan)究(jiu)驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)取(qu)及(ji)(ji)(ji)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)活(huo)性(xing)(xing)(xing)研(yan)(yan)(yan)(yan)究(jiu)： 在源于(yu)驢(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)肽(tai)(tai)(tai)(tai)HP-6對(dui)(dui)(dui)(dui)(dui)造血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)系統的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)體(ti)內(nei)外作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)研(yan)(yan)(yan)(yan)究(jiu)中(zhong)(zhong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)離培(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)人(ren)骨(gu)(gu)髓(sui)有(you)(you)(you)核(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(human bone marrow nucleated cells,hBMNCs)及(ji)(ji)(ji)小(xiao)(xiao)(xiao)鼠(shu)骨(gu)(gu)髓(sui)基(ji)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(murinebone marrow stromal cells,mBMSCs),通(tong)過(guo)(guo)(guo)(guo)與(yu)不(bu)(bu)(bu)同濃度肽(tai)(tai)(tai)(tai)HP-6(0.000 15,0.001 5,0.015,0.15,1.5μmol.L-1)共(gong)培(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)一(yi)(yi)(yi)(yi)(yi)定(ding)時間(jian)考察肽(tai)(tai)(tai)(tai)HP-6對(dui)(dui)(dui)(dui)(dui)骨(gu)(gu)髓(sui)造血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)相(xiang)(xiang)(xiang)關細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)促增(zeng)(zeng)(zeng)(zeng)殖能(neng)(neng)力(li),并通(tong)過(guo)(guo)(guo)(guo)電鏡觀(guan)察作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)后(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)形態;建(jian)立(li)失血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)性(xing)(xing)(xing)貧血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)與(yu)環(huan)磷酰胺(an)致貧血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)2種(zhong)(zhong)貧血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模型(xing),隨機(ji)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)組(zu)(zu)(zu)(zu)并灌(guan)胃不(bu)(bu)(bu)同劑(ji)量(liang)(liang)(liang)(1,0.1,0.01 mg.kg-1)肽(tai)(tai)(tai)(tai)HP-6,同時設立(li)相(xiang)(xiang)(xiang)應(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)對(dui)(dui)(dui)(dui)(dui)照組(zu)(zu)(zu)(zu)灌(guan)胃等(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)(deng)體(ti)積PBS,7 d后(hou)檢(jian)測貧血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)外周(zhou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)象,對(dui)(dui)(dui)(dui)(dui)環(huan)磷酰胺(an)致貧血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)(xiao)鼠(shu)加測骨(gu)(gu)髓(sui)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bone marrow cells,BMCs)數(shu)(shu)(shu)。結(jie)(jie)(jie)果(guo): 肽(tai)(tai)(tai)(tai)HP-6可(ke)(ke)(ke)(ke)(ke)刺激(ji)(ji)人(ren)骨(gu)(gu)髓(sui)有(you)(you)(you)核(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)和(he)(he)(he)小(xiao)(xiao)(xiao)鼠(shu)骨(gu)(gu)髓(sui)基(ji)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)增(zeng)(zeng)(zeng)(zeng)殖且具(ju)有(you)(you)(you)濃度相(xiang)(xiang)(xiang)關性(xing)(xing)(xing),0.15μmol.L-1肽(tai)(tai)(tai)(tai)HP-6使(shi)(shi)(shi)2種(zhong)(zhong)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)均達到(dao)增(zeng)(zeng)(zeng)(zeng)殖率(lv),分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)為152.11%,63.52%,之后(hou)呈(cheng)降低趨勢;電鏡結(jie)(jie)(jie)果(guo)顯示與(yu)肽(tai)(tai)(tai)(tai)HP-6作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)后(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)微(wei)觀(guan)形態結(jie)(jie)(jie)構(gou)正(zheng)常(chang)(chang)(chang);灌(guan)胃1 mg.kg-1肽(tai)(tai)(tai)(tai)HP-6對(dui)(dui)(dui)(dui)(dui)提(ti)(ti)(ti)(ti)(ti)高(gao)(gao)外周(zhou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)(xiao)板(ban)(ban)數(shu)(shu)(shu)量(liang)(liang)(liang)及(ji)(ji)(ji)保(bao)(bao)(bao)護(hu)(hu)環(huan)磷酰胺(an)損(sun)傷(shang)(shang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)小(xiao)(xiao)(xiao)鼠(shu)骨(gu)(gu)髓(sui)具(ju)有(you)(you)(you)一(yi)(yi)(yi)(yi)(yi)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)功效;灌(guan)胃0.1 mg.kg-1肽(tai)(tai)(tai)(tai)HP-6對(dui)(dui)(dui)(dui)(dui)提(ti)(ti)(ti)(ti)(ti)高(gao)(gao)外周(zhou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)(bai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)及(ji)(ji)(ji)紅細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)數(shu)(shu)(shu)量(liang)(liang)(liang)有(you)(you)(you)較好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)效果(guo),還(huan)可(ke)(ke)(ke)(ke)(ke)對(dui)(dui)(dui)(dui)(dui)化(hua)(hua)(hua)(hua)療(liao)藥物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)后(hou)小(xiao)(xiao)(xiao)鼠(shu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)外周(zhou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)起(qi)(qi)到(dao)一(yi)(yi)(yi)(yi)(yi)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)(bao)護(hu)(hu)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong);灌(guan)胃0.01 mg.kg-1肽(tai)(tai)(tai)(tai)HP-6對(dui)(dui)(dui)(dui)(dui)提(ti)(ti)(ti)(ti)(ti)高(gao)(gao)外周(zhou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)(xiao)板(ban)(ban)數(shu)(shu)(shu)量(liang)(liang)(liang)具(ju)有(you)(you)(you)＊＊的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)效果(guo),相(xiang)(xiang)(xiang)對(dui)(dui)(dui)(dui)(dui)增(zeng)(zeng)(zeng)(zeng)殖率(lv)可(ke)(ke)(ke)(ke)(ke)達77.65%。結(jie)(jie)(jie)論:肽(tai)(tai)(tai)(tai)HP-6可(ke)(ke)(ke)(ke)(ke)刺激(ji)(ji)造血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)系統相(xiang)(xiang)(xiang)關細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)增(zeng)(zeng)(zeng)(zeng)殖,對(dui)(dui)(dui)(dui)(dui)小(xiao)(xiao)(xiao)鼠(shu)造血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)功能(neng)(neng)與(yu)抵抗化(hua)(hua)(hua)(hua)療(liao)藥物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)損(sun)傷(shang)(shang)有(you)(you)(you)一(yi)(yi)(yi)(yi)(yi)定(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)增(zeng)(zeng)(zeng)(zeng)強(qiang)作(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)。
豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)（去(qu)紅(hong)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、無(wu)菌(jun)(jun)過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)(lv)(lv)）規(gui)格(ge):500ml/瓶(ping)保(bao)(bao)質(zhi)期: 五(wu)年如(ru)(ru)何解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)才不(bu)(bu)(bu)會(hui)(hui)(hui)(hui)使(shi)(shi)產(chan)品質(zhi)量(liang)(liang)(liang)受(shou)(shou)損? 將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)(cong)冷(leng)凍(dong)(dong)(dong)(dong)(dong)(dong)箱(xiang)(xiang)(xiang)取出(chu)(chu)后(hou)(hou)(hou),先置(zhi)于(yu)(yu)(yu)2-8℃冰(bing)(bing)(bing)(bing)(bing)箱(xiang)(xiang)(xiang)使(shi)(shi)之(zhi)溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)(jie)(jie),然后(hou)(hou)(hou)在(zai)(zai)(zai)(zai)(zai)(zai)室溫(wen)(wen)(wen)(wen)(wen)(wen)下(xia)(xia)使(shi)(shi)之(zhi)全(quan)(quan)溶(rong)(rong)(rong)(rong)。但(dan)(dan)(dan)必(bi)(bi)(bi)須(xu)(xu)注意(yi)(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)是,溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)過(guo)(guo)(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)必(bi)(bi)(bi)須(xu)(xu)規(gui)則(ze)(ze)地(di)搖(yao)晃(huang)(huang)均勻(yun)(yun)。1.實(shi)(shi)驗(yan)(yan)時根(gen)據試(shi)(shi)驗(yan)(yan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)情況和(he)(he)(he)(he)性(xing)(xing)(xing)(xing)質(zhi)進行(xing)必(bi)(bi)(bi)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)防護(hu)(hu)。根(gen)據試(shi)(shi)驗(yan)(yan)可(ke)(ke)(ke)(ke)(ke)能發(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)危險事(shi)(shi)(shi)故(gu)佩戴必(bi)(bi)(bi)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)防護(hu)(hu)工具,例如(ru)(ru)穿好試(shi)(shi)驗(yan)(yan)服,戴橡膠手套(tao),防護(hu)(hu)面具,防毒(du)面具等(deng)(deng)(deng)。實(shi)(shi)驗(yan)(yan)前,要(yao)(yao)(yao)(yao)(yao)(yao)(yao)(yao)注意(yi)(yi)(yi)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)理試(shi)(shi)驗(yan)(yan)場周圍的(de)(de)(de)(de)(de)(de)(de)(de)(de)安(an)全(quan)(quan)隱患。檢(jian)查試(shi)(shi)驗(yan)(yan)裝(zhuang)置(zhi)、藥品和(he)(he)(he)(he)相(xiang)關物(wu)(wu)品是否(fou)有(you)(you)不(bu)(bu)(bu)符(fu)合(he)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)(yao)求的(de)(de)(de)(de)(de)(de)(de)(de)(de)情況等(deng)(deng)(deng)。 2.遵循化(hua)(hua)(hua)學(xue)(xue)(xue)藥品的(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)(xing)(xing)質(zhi)和(he)(he)(he)(he)化(hua)(hua)(hua)學(xue)(xue)(xue)反(fan)(fan)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)規(gui)律,不(bu)(bu)(bu)盲目蠻干(gan)和(he)(he)(he)(he)主(zhu)觀臆測化(hua)(hua)(hua)學(xue)(xue)(xue)反(fan)(fan)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)過(guo)(guo)(guo)(guo)(guo)程。應(ying)(ying)(ying)根(gen)據化(hua)(hua)(hua)學(xue)(xue)(xue)反(fan)(fan)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)(xing)(xing)質(zhi)和(he)(he)(he)(he)過(guo)(guo)(guo)(guo)(guo)程選擇匹配的(de)(de)(de)(de)(de)(de)(de)(de)(de)反(fan)(fan)應(ying)(ying)(ying)裝(zhuang)置(zhi),不(bu)(bu)(bu)可(ke)(ke)(ke)(ke)(ke)圖省(sheng)事(shi)(shi)(shi)省(sheng)去(qu)必(bi)(bi)(bi)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)安(an)全(quan)(quan)措施。 實(shi)(shi)驗(yan)(yan)事(shi)(shi)(shi)故(gu)雖不(bu)(bu)(bu)可(ke)(ke)(ke)(ke)(ke)預(yu)測,但(dan)(dan)(dan)其(qi)危險性(xing)(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)大小(xiao)(xiao)是可(ke)(ke)(ke)(ke)(ke)以(yi)估計到(dao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)。保(bao)(bao)存方(fang)法(fa)(fa)(fa):(1)長(chang)(chang)期保(bao)(bao)存的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必(bi)(bi)(bi)須(xu)(xu)儲存于(yu)(yu)(yu)-20℃ - 70℃ 低(di)(di)溫(wen)(wen)(wen)(wen)(wen)(wen)冰(bing)(bing)(bing)(bing)(bing)箱(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。4℃冰(bing)(bing)(bing)(bing)(bing)箱(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)存時間(jian)(jian)(jian)切(qie)(qie)(qie)勿超過(guo)(guo)(guo)(guo)(guo)1個月(yue)。由(you)于(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結冰(bing)(bing)(bing)(bing)(bing)時體(ti)(ti)(ti)(ti)積(ji)(ji)會(hui)(hui)(hui)(hui)增(zeng)(zeng)加(jia)(jia)約10%,因(yin)(yin)此(ci),血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)(zai)(zai)凍(dong)(dong)(dong)(dong)(dong)(dong)入(ru)(ru)低(di)(di)溫(wen)(wen)(wen)(wen)(wen)(wen)冰(bing)(bing)(bing)(bing)(bing)箱(xiang)(xiang)(xiang)前,必(bi)(bi)(bi)須(xu)(xu)預(yu)留(liu)一(yi)(yi)(yi)(yi)定(ding)體(ti)(ti)(ti)(ti)積(ji)(ji)空(kong)間(jian)(jian)(jian),否(fou)則(ze)(ze)易發(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)污(wu)(wu)染(ran)(ran)或(huo)(huo)(huo)玻璃(li)瓶(ping)凍(dong)(dong)(dong)(dong)(dong)(dong)裂。 (2)一(yi)(yi)(yi)(yi)般(ban) 提(ti)供(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)無(wu)菌(jun)(jun),無(wu)需(xu)(xu)再過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)(lv)(lv)除菌(jun)(jun)。如(ru)(ru)發(fa)(fa)現(xian)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)有(you)(you)懸(xuan)浮物(wu)(wu),則(ze)(ze)可(ke)(ke)(ke)(ke)(ke)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)加(jia)(jia)入(ru)(ru)培(pei)(pei)(pei)(pei)養(yang)液內(nei)(nei)一(yi)(yi)(yi)(yi)起(qi)過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)(lv)(lv),切(qie)(qie)(qie)勿直接過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。(3)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong): 瓶(ping)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)需(xu)(xu)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)逐步(bu)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)法(fa)(fa)(fa):-20℃ 至 -70℃ 低(di)(di)溫(wen)(wen)(wen)(wen)(wen)(wen)冰(bing)(bing)(bing)(bing)(bing)箱(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)放(fang)入(ru)(ru)4℃冰(bing)(bing)(bing)(bing)(bing)箱(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)1天。然后(hou)(hou)(hou)移(yi)入(ru)(ru)室溫(wen)(wen)(wen)(wen)(wen)(wen),待(dai)全(quan)(quan)部(bu)溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)后(hou)(hou)(hou)再分(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)。在(zai)(zai)(zai)(zai)(zai)(zai)溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)過(guo)(guo)(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)需(xu)(xu)不(bu)(bu)(bu)斷(duan)輕輕搖(yao)晃(huang)(huang)均勻(yun)(yun)(小(xiao)(xiao)心(xin)勿造成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)氣(qi)泡),使(shi)(shi)溫(wen)(wen)(wen)(wen)(wen)(wen)度(du)與(yu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)均一(yi)(yi)(yi)(yi),減(jian)少(shao)沉(chen)淀的(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)。切(qie)(qie)(qie)勿直接將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)(cong)-20℃進入(ru)(ru)37℃解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong),這(zhe)(zhe)樣(yang)(yang)因(yin)(yin)溫(wen)(wen)(wen)(wen)(wen)(wen)度(du)改(gai)變(bian)太(tai)大,容(rong)易造成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)凝(ning)(ning)集而(er)出(chu)(chu)現(xian)沉(chen)淀。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)留(liu)應(ying)(ying)(ying)當(dang)(dang)留(liu)意(yi)(yi)(yi)以(yi)下(xia)(xia)幾點(dian):1需(xu)(xu)求長(chang)(chang)時間(jian)(jian)(jian)保(bao)(bao)存的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)有(you)(you)必(bi)(bi)(bi)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)(yao)儲存于(yu)(yu)(yu)-20℃ - 70℃ 低(di)(di)溫(wen)(wen)(wen)(wen)(wen)(wen)冰(bing)(bing)(bing)(bing)(bing)箱(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong).4℃冰(bing)(bing)(bing)(bing)(bing)箱(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)存時間(jian)(jian)(jian)切(qie)(qie)(qie)勿超越1個月(yue).因(yin)(yin)為(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結冰(bing)(bing)(bing)(bing)(bing)時體(ti)(ti)(ti)(ti)積(ji)(ji)會(hui)(hui)(hui)(hui)添加(jia)(jia)約10%,因(yin)(yin)此(ci),血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)(zai)(zai)凍(dong)(dong)(dong)(dong)(dong)(dong)入(ru)(ru)低(di)(di)溫(wen)(wen)(wen)(wen)(wen)(wen)冰(bing)(bing)(bing)(bing)(bing)箱(xiang)(xiang)(xiang)前,有(you)(you)必(bi)(bi)(bi)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)(yao)預(yu)留(liu)必(bi)(bi)(bi)定(ding)體(ti)(ti)(ti)(ti)積(ji)(ji)空(kong)間(jian)(jian)(jian),否(fou)則(ze)(ze)易發(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)污(wu)(wu)染(ran)(ran)或(huo)(huo)(huo)玻璃(li)瓶(ping)凍(dong)(dong)(dong)(dong)(dong)(dong)裂.2.熱(re)(re)滅(mie)(mie)(mie)活(huo)(huo)是指(zhi)56℃, 30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘加(jia)(jia)熱(re)(re)已完全(quan)(quan)凍(dong)(dong)(dong)(dong)(dong)(dong)住的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).加(jia)(jia)熱(re)(re)過(guo)(guo)(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)須(xu)(xu)規(gui)矩(ju)搖(yao)晃(huang)(huang)均勻(yun)(yun).此(ci)熱(re)(re)處(chu)理的(de)(de)(de)(de)(de)(de)(de)(de)(de)目的(de)(de)(de)(de)(de)(de)(de)(de)(de)是使(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)補(bu)體(ti)(ti)(ti)(ti)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(complement)滅(mie)(mie)(mie)活(huo)(huo).除非(fei)有(you)(you)必(bi)(bi)(bi)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)(yao),一(yi)(yi)(yi)(yi)般(ban)不(bu)(bu)(bu)建(jian)議(yi)作(zuo)此(ci)熱(re)(re)處(chu)理,因(yin)(yin)為(wei)熱(re)(re)處(chu)理會(hui)(hui)(hui)(hui)構(gou)(gou)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)堆(dui)(dui)積(ji)(ji)物(wu)(wu)明(ming)顯(xian)增(zeng)(zeng)多(duo),而(er)且還會(hui)(hui)(hui)(hui)影(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)量(liang)(liang)(liang).補(bu)體(ti)(ti)(ti)(ti)參(can)與(yu)反(fan)(fan)響有(you)(you):細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)毒(du)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong), 滑潤肌細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)縮短(duan), 肥大細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)和(he)(he)(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)板(ban)開釋(shi)組(zu)(zu)胺, 增(zeng)(zeng)強吞噬作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong), 推動和(he)(he)(he)(he)發(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)學(xue)(xue)(xue)趨化(hua)(hua)(hua)和(he)(he)(he)(he)活(huo)(huo)化(hua)(hua)(hua).3.瓶(ping)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)凍(dong)(dong)(dong)(dong)(dong)(dong)住需(xu)(xu)選用(yong)(yong)(yong)(yong)(yong)(yong)(yong)逐步(bu)凍(dong)(dong)(dong)(dong)(dong)(dong)住法(fa)(fa)(fa):-20℃ 至 -70℃ 低(di)(di)溫(wen)(wen)(wen)(wen)(wen)(wen)冰(bing)(bing)(bing)(bing)(bing)箱(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)放(fang)入(ru)(ru)4℃冰(bing)(bing)(bing)(bing)(bing)箱(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)1天.然后(hou)(hou)(hou)移(yi)入(ru)(ru)室溫(wen)(wen)(wen)(wen)(wen)(wen),待(dai)全(quan)(quan)部(bu)溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)后(hou)(hou)(hou)再分(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang).在(zai)(zai)(zai)(zai)(zai)(zai)溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)過(guo)(guo)(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)需(xu)(xu)不(bu)(bu)(bu)斷(duan)悄悄搖(yao)晃(huang)(huang)均勻(yun)(yun)(當(dang)(dang)心(xin)勿構(gou)(gou)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)氣(qi)泡),使(shi)(shi)溫(wen)(wen)(wen)(wen)(wen)(wen)度(du)與(yu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)均一(yi)(yi)(yi)(yi),削減(jian)堆(dui)(dui)積(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng).切(qie)(qie)(qie)勿直接將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)(cong)-20℃進入(ru)(ru)37℃凍(dong)(dong)(dong)(dong)(dong)(dong)住,這(zhe)(zhe)么(me)因(yin)(yin)溫(wen)(wen)(wen)(wen)(wen)(wen)度(du)改(gai)動太(tai)大,簡略構(gou)(gou)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)凝(ning)(ning)集而(er)出(chu)(chu)現(xian)堆(dui)(dui)積(ji)(ji).4.一(yi)(yi)(yi)(yi)般(ban) 供(gong)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)無(wu)菌(jun)(jun),無(wu)需(xu)(xu)再過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)(lv)(lv)除菌(jun)(jun).如(ru)(ru)發(fa)(fa)現(xian)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)有(you)(you)懸(xuan)浮物(wu)(wu),則(ze)(ze)可(ke)(ke)(ke)(ke)(ke)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)參(can)加(jia)(jia)培(pei)(pei)(pei)(pei)養(yang)液內(nei)(nei)一(yi)(yi)(yi)(yi)同過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)(lv)(lv),切(qie)(qie)(qie)勿直接過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).5.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)堆(dui)(dui)積(ji)(ji)物(wu)(wu) 絮(xu)(xu)(xu)狀物(wu)(wu):主(zhu)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)(yao)是血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)脂(zhi)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)變(bian)性(xing)(xing)(xing)(xing)及凍(dong)(dong)(dong)(dong)(dong)(dong)住后(hou)(hou)(hou)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)纖(xian)維蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)構(gou)(gou)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng),這(zhe)(zhe)些(xie)(xie)絮(xu)(xu)(xu)狀物(wu)(wu)不(bu)(bu)(bu)會(hui)(hui)(hui)(hui)影(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本身的(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)量(liang)(liang)(liang).可(ke)(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)離心(xin)3000rpm, 5分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘去(qu)掉,也可(ke)(ke)(ke)(ke)(ke)不(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)處(chu)理. 顯(xian)微鏡下(xia)(xia)"小(xiao)(xiao)黑點(dian)":通過(guo)(guo)(guo)(guo)(guo)熱(re)(re)處(chu)理過(guo)(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),堆(dui)(dui)積(ji)(ji)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)構(gou)(gou)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)會(hui)(hui)(hui)(hui)明(ming)顯(xian)增(zeng)(zeng)多(duo).有(you)(you)些(xie)(xie)堆(dui)(dui)積(ji)(ji)物(wu)(wu)在(zai)(zai)(zai)(zai)(zai)(zai)顯(xian)微鏡下(xia)(xia)查詢象(xiang)"小(xiao)(xiao)黑點(dian)",常(chang)(chang)(chang)誤認為(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)受(shou)(shou)污(wu)(wu)染(ran)(ran).一(yi)(yi)(yi)(yi)般(ban)情況下(xia)(xia),此(ci)小(xiao)(xiao)黑點(dian)不(bu)(bu)(bu)會(hui)(hui)(hui)(hui)影(ying)響細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang),但(dan)(dan)(dan)假如(ru)(ru)懷疑血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)量(liang)(liang)(liang),則(ze)(ze)應(ying)(ying)(ying)當(dang)(dang)即停(ting)止(zhi)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong),替換另一(yi)(yi)(yi)(yi)批(pi)號的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing).6.切(qie)(qie)(qie)勿將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)(zai)(zai)37℃放(fang)置(zhi)太(tai)久,否(fou)則(ze)(ze)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)會(hui)(hui)(hui)(hui)變(bian)得渾(hun)濁,一(yi)(yi)(yi)(yi)同血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)效(xiao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)會(hui)(hui)(hui)(hui)破會(hui)(hui)(hui)(hui)而(er)影(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)量(liang)(liang)(liang)背景豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)系(xi)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)年健康豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液為(wei)原(yuan)(yuan)料加(jia)(jia)工而(er)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)，經(jing)無(wu)菌(jun)(jun)采(cai)集、分(fen)(fen)(fen)(fen)(fen)(fen)(fen)離、微孔(kong)過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)(lv)(lv)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)而(er)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)。無(wu)支原(yuan)(yuan)體(ti)(ti)(ti)(ti)、無(wu)噬菌(jun)(jun)體(ti)(ti)(ti)(ti)，內(nei)(nei)毒(du)素(su)(su)(su)(su)含(han)量(liang)(liang)(liang)低(di)(di)于(yu)(yu)(yu)50EU/ml。豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（無(wu)菌(jun)(jun)過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)(lv)(lv)）在(zai)(zai)(zai)(zai)(zai)(zai)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)培(pei)(pei)(pei)(pei)養(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)：1.提(ti)供(gong)基(ji)本營(ying)養(yang)物(wu)(wu)質(zhi)：氨(an)基(ji)酸(suan)、維生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素(su)(su)(su)(su)、無(wu)機(ji)(ji)物(wu)(wu)、脂(zhi)類(lei)物(wu)(wu)質(zhi)、核酸(suan)衍生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)等(deng)(deng)(deng)，是細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)必(bi)(bi)(bi)須(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)質(zhi)。2.提(ti)供(gong)激(ji)素(su)(su)(su)(su)和(he)(he)(he)(he)各種(zhong)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)(yin)子：胰(yi)島素(su)(su)(su)(su)、腎(shen)上腺(xian)皮(pi)質(zhi)激(ji)素(su)(su)(su)(su)（地(di)塞米松）、類(lei)固(gu)醇(chun)激(ji)素(su)(su)(su)(su)（雌二醇(chun)、睪(gao)酮、孕酮）等(deng)(deng)(deng)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)(yin)子如(ru)(ru)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)纖(xian)維細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)(yin)子、表皮(pi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)(yin)子、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)板(ban)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)因(yin)(yin)子等(deng)(deng)(deng)。3.提(ti)供(gong)結合(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)：結合(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)是攜(xie)帶(dai)重(zhong)(zhong)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)(yao)地(di)低(di)(di)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)子量(liang)(liang)(liang)物(wu)(wu)質(zhi)，如(ru)(ru)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜(xie)帶(dai)維生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素(su)(su)(su)(su)、脂(zhi)肪、以(yi)及激(ji)素(su)(su)(su)(su)等(deng)(deng)(deng)，轉鐵(tie)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)攜(xie)帶(dai)鐵(tie)。結合(he)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)在(zai)(zai)(zai)(zai)(zai)(zai)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)代謝過(guo)(guo)(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)起(qi)重(zhong)(zhong)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)(yao)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。提(ti)供(gong)促接觸和(he)(he)(he)(he)伸展因(yin)(yin)子使(shi)(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)壁免(mian)受(shou)(shou)機(ji)(ji)械損傷。4.對(dui)培(pei)(pei)(pei)(pei)養(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)起(qi)到(dao)某些(xie)(xie)保(bao)(bao)護(hu)(hu)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)：有(you)(you)一(yi)(yi)(yi)(yi)些(xie)(xie)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)，如(ru)(ru)內(nei)(nei)皮(pi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、骨(gu)髓樣(yang)(yang)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)可(ke)(ke)(ke)(ke)(ke)以(yi)釋(shi)放(fang)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)含(han)有(you)(you)抗蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)，起(qi)到(dao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。這(zhe)(zhe)種(zhong)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)是偶然發(fa)(fa)現(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)，現(xian)在(zai)(zai)(zai)(zai)(zai)(zai)則(ze)(ze)有(you)(you)目的(de)(de)(de)(de)(de)(de)(de)(de)(de)的(de)(de)(de)(de)(de)(de)(de)(de)(de)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)來終(zhong)止(zhi)胰(yi)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶的(de)(de)(de)(de)(de)(de)(de)(de)(de)消化(hua)(hua)(hua)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。因(yin)(yin)為(wei)胰(yi)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)酶已經(jing)被(bei)廣泛用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)貼(tie)壁細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)消化(hua)(hua)(hua)傳代。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)形成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)了(le)(le)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)粘度(du)，可(ke)(ke)(ke)(ke)(ke)以(yi)保(bao)(bao)護(hu)(hu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)免(mian)受(shou)(shou)機(ji)(ji)械損傷，特別是在(zai)(zai)(zai)(zai)(zai)(zai)懸(xuan)浮培(pei)(pei)(pei)(pei)養(yang)攪拌時，粘度(du)起(qi)到(dao)重(zhong)(zhong)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)(yao)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)還含(han)有(you)(you)一(yi)(yi)(yi)(yi)些(xie)(xie)微量(liang)(liang)(liang)元素(su)(su)(su)(su)和(he)(he)(he)(he)離子，他(ta)們在(zai)(zai)(zai)(zai)(zai)(zai)代謝解(jie)(jie)(jie)(jie)(jie)(jie)(jie)毒(du)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)起(qi)重(zhong)(zhong)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)(yao)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)SeO3,硒等(deng)(deng)(deng)。豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)（去(qu)紅(hong)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)、無(wu)菌(jun)(jun)過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)(lv)(lv)）使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)注意(yi)(yi)(yi)事(shi)(shi)(shi)項1.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)逐步(bu)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)法(fa)(fa)(fa)（-20℃→2—8℃→25℃或(huo)(huo)(huo)37℃），解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)過(guo)(guo)(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)必(bi)(bi)(bi)須(xu)(xu)隨時輕搖(yao)，使(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)受(shou)(shou)熱(re)(re)溫(wen)(wen)(wen)(wen)(wen)(wen)度(du)均勻(yun)(yun)。2. 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)凝(ning)(ning)絮(xu)(xu)(xu)物(wu)(wu)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)后(hou)(hou)(hou)會(hui)(hui)(hui)(hui)出(chu)(chu)現(xian)少(shao)量(liang)(liang)(liang)片狀或(huo)(huo)(huo)絮(xu)(xu)(xu)狀物(wu)(wu)析出(chu)(chu)，這(zhe)(zhe)主(zhu)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)(yao)是由(you)于(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)內(nei)(nei)不(bu)(bu)(bu)穩定(ding)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)變(bian)性(xing)(xing)(xing)(xing)、纖(xian)維蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)析出(chu)(chu)形成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)沉(chen)淀物(wu)(wu)，實(shi)(shi)驗(yan)(yan)證明(ming)沉(chen)淀物(wu)(wu)不(bu)(bu)(bu)會(hui)(hui)(hui)(hui)影(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本身質(zhi)量(liang)(liang)(liang)。;3. 熱(re)(re)滅(mie)(mie)(mie)活(huo)(huo)本產(chan)品除注明(ming)外均未滅(mie)(mie)(mie)活(huo)(huo)。如(ru)(ru)果必(bi)(bi)(bi)須(xu)(xu)滅(mie)(mie)(mie)活(huo)(huo)，請(qing)嚴格(ge)按照56℃30分(fen)(fen)(fen)(fen)(fen)(fen)(fen)鐘（水浴(yu)）處(chu)理已解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。4. 常(chang)(chang)(chang)溫(wen)(wen)(wen)(wen)(wen)(wen)狀態下(xia)(xia)短(duan)期融(rong)化(hua)(hua)(hua)并不(bu)(bu)(bu)會(hui)(hui)(hui)(hui)影(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)量(liang)(liang)(liang)。5.產(chan)品有(you)(you)效(xiao)期，檢(jian)定(ding)合(he)格(ge)之(zhi)日起(qi)有(you)(you)效(xiao)期5年。解(jie)(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)：將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)(cong)冷(leng)凍(dong)(dong)(dong)(dong)(dong)(dong)箱(xiang)(xiang)(xiang)取出(chu)(chu)后(hou)(hou)(hou)，先置(zhi)于(yu)(yu)(yu)2-8℃冰(bing)(bing)(bing)(bing)(bing)箱(xiang)(xiang)(xiang)使(shi)(shi)之(zhi)溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)，然后(hou)(hou)(hou)在(zai)(zai)(zai)(zai)(zai)(zai)室溫(wen)(wen)(wen)(wen)(wen)(wen)下(xia)(xia)使(shi)(shi)之(zhi)全(quan)(quan)溶(rong)(rong)(rong)(rong)。但(dan)(dan)(dan)必(bi)(bi)(bi)須(xu)(xu)注意(yi)(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)是，溶(rong)(rong)(rong)(rong)解(jie)(jie)(jie)(jie)(jie)(jie)(jie)過(guo)(guo)(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)必(bi)(bi)(bi)須(xu)(xu)規(gui)則(ze)(ze)地(di)搖(yao)晃(huang)(huang)均勻(yun)(yun)。二、保(bao)(bao)存血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)應(ying)(ying)(ying)保(bao)(bao)存在(zai)(zai)(zai)(zai)(zai)(zai)-5℃至-2O℃若存放(fang)于(yu)(yu)(yu)4℃時，請(qing)勿超過(guo)(guo)(guo)(guo)(guo)一(yi)(yi)(yi)(yi)個月(yue)。若一(yi)(yi)(yi)(yi)次無(wu)法(fa)(fa)(fa)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)完一(yi)(yi)(yi)(yi)瓶(ping)，建(jian)議(yi)將(jiang)無(wu)菌(jun)(jun)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)至恰當(dang)(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)滅(mie)(mie)(mie)菌(jun)(jun)容(rong)器內(nei)(nei)，再放(fang)回冷(leng)凍(dong)(dong)(dong)(dong)(dong)(dong)。豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（無(wu)菌(jun)(jun)過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)(lv)(lv)）注意(yi)(yi)(yi)事(shi)(shi)(shi)項：（1）有(you)(you)少(shao)量(liang)(liang)(liang)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)質(zhi)結塊析出(chu)(chu)不(bu)(bu)(bu)影(ying)響使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。未融(rong)化(hua)(hua)(hua)或(huo)(huo)(huo)融(rong)化(hua)(hua)(hua)未經(jing)搖(yao)勻(yun)(yun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)禁止(zhi)直接放(fang)入(ru)(ru)高溫(wen)(wen)(wen)(wen)(wen)(wen)水浴(yu)內(nei)(nei)加(jia)(jia)溫(wen)(wen)(wen)(wen)(wen)(wen)。減(jian)少(shao)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)重(zhong)(zhong)復凍(dong)(dong)(dong)(dong)(dong)(dong)融(rong)。若您(nin)一(yi)(yi)(yi)(yi)次無(wu)法(fa)(fa)(fa)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)完一(yi)(yi)(yi)(yi)瓶(ping)，建(jian)議(yi)您(nin)無(wu)菌(jun)(jun)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)至恰當(dang)(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)滅(mie)(mie)(mie)菌(jun)(jun)容(rong)器內(nei)(nei)，再放(fang)回冷(leng)凍(dong)(dong)(dong)(dong)(dong)(dong)。（2）長(chang)(chang)期保(bao)(bao)存的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)必(bi)(bi)(bi)須(xu)(xu)儲存于(yu)(yu)(yu)-20℃-70℃低(di)(di)溫(wen)(wen)(wen)(wen)(wen)(wen)冰(bing)(bing)(bing)(bing)(bing)箱(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。4℃冰(bing)(bing)(bing)(bing)(bing)箱(xiang)(xiang)(xiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)保(bao)(bao)存時間(jian)(jian)(jian)切(qie)(qie)(qie)勿超過(guo)(guo)(guo)(guo)(guo)1個月(yue)。由(you)于(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)結冰(bing)(bing)(bing)(bing)(bing)時體(ti)(ti)(ti)(ti)積(ji)(ji)會(hui)(hui)(hui)(hui)增(zeng)(zeng)加(jia)(jia)約10%,因(yin)(yin)此(ci)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)(zai)(zai)凍(dong)(dong)(dong)(dong)(dong)(dong)入(ru)(ru)低(di)(di)溫(wen)(wen)(wen)(wen)(wen)(wen)冰(bing)(bing)(bing)(bing)(bing)箱(xiang)(xiang)(xiang)前，必(bi)(bi)(bi)須(xu)(xu)預(yu)留(liu)一(yi)(yi)(yi)(yi)定(ding)體(ti)(ti)(ti)(ti)積(ji)(ji)空(kong)間(jian)(jian)(jian)，否(fou)則(ze)(ze)易發(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)污(wu)(wu)染(ran)(ran)或(huo)(huo)(huo)玻璃(li)瓶(ping)凍(dong)(dong)(dong)(dong)(dong)(dong)裂。（3）一(yi)(yi)(yi)(yi)般(ban)廠(chang)商提(ti)供(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為(wei)無(wu)菌(jun)(jun)，無(wu)需(xu)(xu)再過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)(lv)(lv)除菌(jun)(jun)。如(ru)(ru)發(fa)(fa)現(xian)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)有(you)(you)懸(xuan)浮物(wu)(wu)，則(ze)(ze)可(ke)(ke)(ke)(ke)(ke)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)加(jia)(jia)入(ru)(ru)培(pei)(pei)(pei)(pei)養(yang)液內(nei)(nei)一(yi)(yi)(yi)(yi)起(qi)過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)(lv)(lv)，切(qie)(qie)(qie)勿直接過(guo)(guo)(guo)(guo)(guo)濾(lv)(lv)(lv)(lv)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。介(jie)紹 疫(yi)(yi)(yi)苗(miao)(miao)在(zai)(zai)(zai)(zai)(zai)(zai)預(yu)防豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)常(chang)(chang)(chang)見(jian)傳染(ran)(ran)病(bing)(bing)方(fang)面起(qi)著非(fei)常(chang)(chang)(chang)重(zhong)(zhong)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。 它包括(kuo)豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)瘟(wen)(wen)(wen)疫(yi)(yi)(yi)苗(miao)(miao)、豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)肺炎(yan)支原(yuan)(yuan)體(ti)(ti)(ti)(ti)疫(yi)(yi)(yi)苗(miao)(miao)以(yi)及正處(chu)在(zai)(zai)(zai)(zai)(zai)(zai)研發(fa)(fa)熱(re)(re)點(dian)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)非(fei)洲豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)瘟(wen)(wen)(wen)等(deng)(deng)(deng)疫(yi)(yi)(yi)苗(miao)(miao)。 在(zai)(zai)(zai)(zai)(zai)(zai)豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)病(bing)(bing)毒(du)疫(yi)(yi)(yi)苗(miao)(miao)和(he)(he)(he)(he)肺炎(yan)支原(yuan)(yuan)體(ti)(ti)(ti)(ti)疫(yi)(yi)(yi)苗(miao)(miao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是常(chang)(chang)(chang)見(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)養(yang)液成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)。 尤其(qi)是豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)肺炎(yan)支原(yuan)(yuan)體(ti)(ti)(ti)(ti)疫(yi)(yi)(yi)苗(miao)(miao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)。在(zai)(zai)(zai)(zai)(zai)(zai)對(dui)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)和(he)(he)(he)(he)活(huo)(huo)疫(yi)(yi)(yi)苗(miao)(miao)進行(xing)支原(yuan)(yuan)體(ti)(ti)(ti)(ti)檢(jian)驗(yan)(yan)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，《獸(shou)藥典(dian)》也明(ming)確推薦了(le)(le)豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)作(zuo)為(wei)支原(yuan)(yuan)體(ti)(ti)(ti)(ti)檢(jian)驗(yan)(yan)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)液體(ti)(ti)(ti)(ti)培(pei)(pei)(pei)(pei)養(yang)基(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)。并且，《獸(shou)藥典(dian)》指(zhi)出(chu)(chu)在(zai)(zai)(zai)(zai)(zai)(zai)【豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)支原(yuan)(yuan)體(ti)(ti)(ti)(ti)肺炎(yan)微量(liang)(liang)(liang)間(jian)(jian)(jian)接血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)凝(ning)(ning)試(shi)(shi)驗(yan)(yan)】和(he)(he)(he)(he)【豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)支氣(qi)管敗血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)波氏桿菌(jun)(jun)凝(ning)(ning)集試(shi)(shi)驗(yan)(yan)】中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)需(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)(yao)(yao)陰性(xing)(xing)(xing)(xing)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，即正常(chang)(chang)(chang)豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。 在(zai)(zai)(zai)(zai)(zai)(zai)豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)瘟(wen)(wen)(wen)疫(yi)(yi)(yi)苗(miao)(miao)等(deng)(deng)(deng)其(qi)他(ta)豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)病(bing)(bing)毒(du)疫(yi)(yi)(yi)苗(miao)(miao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)腎(shen)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)（PK-15）或(huo)(huo)(huo)豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)睪(gao)丸細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)（ST）進行(xing)病(bing)(bing)毒(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)繁殖。其(qi)培(pei)(pei)(pei)(pei)養(yang)液除添加(jia)(jia)MEM外，常(chang)(chang)(chang)添加(jia)(jia)新生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)牛(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)。但(dan)(dan)(dan)從(cong)(cong)品系(xi)相(xiang)同的(de)(de)(de)(de)(de)(de)(de)(de)(de)角度(du)來看(kan)，豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)更為(wei)合(he)適。 實(shi)(shi)際上早(zao)在(zai)(zai)(zai)(zai)(zai)(zai)1989年，國(guo)外就(jiu)建(jian)議(yi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)取代牛(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)培(pei)(pei)(pei)(pei)養(yang)制造非(fei)洲豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)瘟(wen)(wen)(wen)病(bing)(bing)毒(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)（見(jian)《動物(wu)(wu)檢(jian)疫(yi)(yi)(yi)》 1991年01期《豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)牛(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)抗體(ti)(ti)(ti)(ti)引(yin)起(qi)非(fei)洲豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)瘟(wen)(wen)(wen)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)學(xue)(xue)(xue)診斷(duan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)假陽(yang)性(xing)(xing)(xing)(xing)反(fan)(fan)應(ying)(ying)(ying)》），原(yuan)(yuan)因(yin)(yin)是牛(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)(bai)(bai)會(hui)(hui)(hui)(hui)干(gan)擾非(fei)洲豬(zhu)(zhu)(zhu)(zhu)(zhu)(zhu)瘟(wen)(wen)(wen)抗體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)測，造成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)假陽(yang)性(xing)(xing)(xing)(xing)反(fan)(fan)應(ying)(ying)(ying)。
豚(tun)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)（去(qu)(qu)紅細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)、無(wu)(wu)(wu)菌(jun)過(guo)(guo)濾）血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)注(zhu)(zhu)意(yi)(yi)事(shi)項(xiang)：防止(zhi)(zhi)發病免(mian)疫(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)多(duo)(duo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)馬(ma)、牛(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)制(zhi)備，馬(ma)、牛(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)對(dui)(dui)其(qi)(qi)它(ta)動物(wu)(wu)(wu)(wu)來(lai)說，也具有(you)(you)抗原(yuan)(yuan)(yuan)性(xing)，有(you)(you)引(yin)起(qi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)可(ke)(ke)(ke)(ke)(ke)能(neng)，因(yin)(yin)此(ci)，使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)免(mian)疫(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)要(yao)(yao)(yao)注(zhu)(zhu)意(yi)(yi)防止(zhi)(zhi)引(yin)起(qi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病，預防的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)要(yao)(yao)(yao)措施(shi)是(shi)(shi)(shi)(shi)(shi)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)提純的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)品，不(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)不(bu)合(he)格的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)品；另外，不(bu)要(yao)(yao)(yao)有(you)(you)急功(gong)近利的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)想法(fa)(fa)，要(yao)(yao)(yao)按照(zhao)要(yao)(yao)(yao)求劑量(liang)(liang)(liang)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，一(yi)(yi)(yi)次(ci)(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)量(liang)(liang)(liang)不(bu)可(ke)(ke)(ke)(ke)(ke)過(guo)(guo)大(da)。大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)取(qu)(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)操作(zuo)(zuo)步驟：1.剪(jian)尾(wei)采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue) 小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)每(mei)次(ci)(ci)采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)0.1ml，大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)每(mei)次(ci)(ci)采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)0.3-0.5ml左(zuo)(zuo)手拇指和(he)(he)食指從背部(bu)(bu)(bu)(bu)捉(zhuo)住(zhu)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)頸部(bu)(bu)(bu)(bu)皮膚(fu)，將(jiang)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)頭(tou)朝下(xia)(xia)(xia)(xia)(xia)(xia)，鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)保(bao)(bao)(bao)定(ding)后(hou)(hou)將(jiang)其(qi)(qi)尾(wei)巴置于(yu)50℃熱水中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)浸(jin)(jin)泡數(shu)分(fen)(fen)(fen)鐘，使(shi)(shi)(shi)尾(wei)部(bu)(bu)(bu)(bu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管(guan)充(chong)盈。擦干尾(wei)部(bu)(bu)(bu)(bu)，再用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)剪(jian)刀(dao)或刀(dao)片剪(jian)去(qu)(qu)尾(wei)尖1-2mm，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)試管(guan)接流(liu)(liu)出(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)，同時自尾(wei)根部(bu)(bu)(bu)(bu)向尾(wei)尖按-摩。取(qu)(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后(hou)(hou)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)棉(mian)球(qiu)壓(ya)(ya)榨(zha)止(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)并(bing)(bing)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)6%液(ye)(ye)體(ti)火棉(mian)膠(jiao)涂在(zai)(zai)(zai)(zai)(zai)傷口處(chu)(chu)止(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。 2.去(qu)(qu)除眼(yan)(yan)(yan)球(qiu)采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)0.5-1ml左(zuo)(zuo)手捉(zhuo)住(zhu)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)頸部(bu)(bu)(bu)(bu)皮膚(fu)，輕壓(ya)(ya)在(zai)(zai)(zai)(zai)(zai)實(shi)驗(yan)臺上，取(qu)(qu)(qu)側臥(wo)位，左(zuo)(zuo)手食指盡(jin)量(liang)(liang)(liang)將(jiang)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)眼(yan)(yan)(yan)周皮膚(fu)往(wang)頸后(hou)(hou)壓(ya)(ya)，使(shi)(shi)(shi)眼(yan)(yan)(yan)球(qiu)杰出(chu)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)眼(yan)(yan)(yan)科彎鑷迅(xun)速夾去(qu)(qu)眼(yan)(yan)(yan)球(qiu)，將(jiang)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)倒立，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)器(qi)皿接住(zhu)流(liu)(liu)出(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)。采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)完畢立即(ji)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)紗布壓(ya)(ya)榨(zha)止(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)少(shao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。 3.心(xin)臟(zang)(zang)采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)0.5-0.6ml，大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)1-1.5ml鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)仰臥(wo)位固定(ding)，剪(jian)去(qu)(qu)胸前(qian)區被(bei)毛(mao)，皮膚(fu)消(xiao)毒后(hou)(hou)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)左(zuo)(zuo)手食指在(zai)(zai)(zai)(zai)(zai)左(zuo)(zuo)側第(di)3-4肋間觸摸(mo)到(dao)心(xin)搏處(chu)(chu)，右(you)手持帶(dai)(dai)有(you)(you)4-5號針頭(tou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)注(zhu)(zhu)射器(qi)，選(xuan)擇心(xin)搏zui強處(chu)(chu)穿刺(ci)，當刺(ci)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)心(xin)臟(zang)(zang)時，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)會(hui)(hui)主(zhu)(zhu)動進(jin)(jin)(jin)入(ru)注(zhu)(zhu)射器(qi)。 4.斷(duan)頭(tou)采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.8-1.2ml，大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)5-10ml左(zuo)(zuo)手拇指和(he)(he)食指從背部(bu)(bu)(bu)(bu)捉(zhuo)住(zhu)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)頸部(bu)(bu)(bu)(bu)皮膚(fu)，將(jiang)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)頭(tou)朝下(xia)(xia)(xia)(xia)(xia)(xia)，右(you)手用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)剪(jian)刀(dao)剪(jian)斷(duan)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)頸部(bu)(bu)(bu)(bu)約1/2-4/5，讓(rang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)流(liu)(liu)入(ru)試管(guan)。 5.眼(yan)(yan)(yan)眶靜(jing)脈叢(cong)采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)為(wei)(wei)(wei)0.2-0.4ml，大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)為(wei)(wei)(wei)0.4-0.8ml取(qu)(qu)(qu)內徑為(wei)(wei)(wei)1.0-1.5mm的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)玻(bo)璃毛(mao)細(xi)(xi)(xi)(xi)管(guan)，臨用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)折斷(duan)成(cheng)(cheng)(cheng)1～1.5cm長(chang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)毛(mao)細(xi)(xi)(xi)(xi)管(guan)段(duan)，浸(jin)(jin)入(ru)1％肝素(su)溶液(ye)(ye)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，干燥后(hou)(hou)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。取(qu)(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)時左(zuo)(zuo)手捉(zhuo)住(zhu)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)兩耳(er)之(zhi)間的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)頸背部(bu)(bu)(bu)(bu)皮膚(fu)以(yi)(yi)固定(ding)頭(tou)部(bu)(bu)(bu)(bu)，悄(qiao)(qiao)悄(qiao)(qiao)向下(xia)(xia)(xia)(xia)(xia)(xia)壓(ya)(ya)榨(zha)頸部(bu)(bu)(bu)(bu)兩邊，引(yin)起(qi)頭(tou)部(bu)(bu)(bu)(bu)靜(jing)脈血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)回流(liu)(liu)困(kun)難使(shi)(shi)(shi)眼(yan)(yan)(yan)眶靜(jing)脈叢(cong)充(chong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)，右(you)手持毛(mao)細(xi)(xi)(xi)(xi)管(guan)由內眥部(bu)(bu)(bu)(bu)刺(ci)進(jin)(jin)(jin)結膜(mo)，再悄(qiao)(qiao)悄(qiao)(qiao)向眼(yan)(yan)(yan)底部(bu)(bu)(bu)(bu)方(fang)向推進(jin)(jin)(jin)，悄(qiao)(qiao)悄(qiao)(qiao)旋轉(zhuan)毛(mao)細(xi)(xi)(xi)(xi)管(guan)以(yi)(yi)劃(hua)破(po)靜(jing)脈叢(cong)，讓(rang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)順毛(mao)細(xi)(xi)(xi)(xi)管(guan)流(liu)(liu)出(chu)，接收入(ru)事(shi)前(qian)準備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)容器(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)。采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后(hou)(hou)紗布輕壓(ya)(ya)眼(yan)(yan)(yan)部(bu)(bu)(bu)(bu)止(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)、大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)、豚(tun)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)及家兔均(jun)可(ke)(ke)(ke)(ke)(ke)采(cai)納此(ci)法(fa)(fa)取(qu)(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。刺(ci)入(ru)深(shen)度(du)(du)小(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)為(wei)(wei)(wei)2-3mm，可(ke)(ke)(ke)(ke)(ke)采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.2-0.3ml；大(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)為(wei)(wei)(wei)4-5mm，可(ke)(ke)(ke)(ke)(ke)采(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.4-0.8ml。豚(tun)鼠(shu)(shu)(shu)(shu)(shu)(shu)(shu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)（去(qu)(qu)紅細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)、無(wu)(wu)(wu)菌(jun)過(guo)(guo)濾）Tips：1. 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)(bao)存(cun)：在(zai)(zai)(zai)(zai)(zai)-15℃以(yi)(yi)下(xia)(xia)(xia)(xia)(xia)(xia)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)效期為(wei)(wei)(wei)5年，4℃可(ke)(ke)(ke)(ke)(ke)保(bao)(bao)(bao)存(cun)數(shu)周，不(bu)宜室(shi)溫保(bao)(bao)(bao)存(cun)，不(bu)宜反(fan)復凍(dong)融。配制(zhi)成(cheng)(cheng)(cheng)完全(quan)培(pei)養(yang)基(ji)后(hou)(hou)應(ying)在(zai)(zai)(zai)(zai)(zai)四周內用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完，一(yi)(yi)(yi)瓶(ping)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)如(ru)無(wu)(wu)(wu)法(fa)(fa)一(yi)(yi)(yi)次(ci)(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完應(ying)無(wu)(wu)(wu)菌(jun)分(fen)(fen)(fen)裝成(cheng)(cheng)(cheng)恰當數(shu)量(liang)(liang)(liang)并(bing)(bing)冷凍(dong)保(bao)(bao)(bao)存(cun)。2、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)絮狀沉(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)多(duo)(duo)種(zhong)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混(hun)合(he)物(wu)(wu)(wu)(wu)，在(zai)(zai)(zai)(zai)(zai)凍(dong)融過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)會(hui)(hui)出(chu)現(xian)(xian)絮狀沉(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)，主(zhu)(zhu)要(yao)(yao)(yao)原(yuan)(yuan)(yuan)因(yin)(yin)是(shi)(shi)(shi)(shi)(shi)由于(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)脂(zhi)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)和(he)(he)纖維蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)變性(xing)所造成(cheng)(cheng)(cheng)。但(dan)并(bing)(bing)不(bu)影(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本(ben)身(shen)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)量(liang)(liang)(liang)和(he)(he)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。如(ru)需(xu)去(qu)(qu)除可(ke)(ke)(ke)(ke)(ke)先400g離心(xin)取(qu)(qu)(qu)上清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)配制(zhi)成(cheng)(cheng)(cheng)完全(quan)培(pei)養(yang)基(ji)再過(guo)(guo)濾即(ji)可(ke)(ke)(ke)(ke)(ke)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。3、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)凍(dong)：合(he)理的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)凍(dong)方(fang)式可(ke)(ke)(ke)(ke)(ke)以(yi)(yi)更好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)(bao)護血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)量(liang)(liang)(liang)并(bing)(bing)可(ke)(ke)(ke)(ke)(ke)以(yi)(yi)在(zai)(zai)(zai)(zai)(zai)＊大(da)程度(du)(du)上減少(shao)絮狀沉(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)生(sheng)(sheng)(sheng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從低(di)溫冰(bing)箱中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)取(qu)(qu)(qu)出(chu)后(hou)(hou)，經4℃冰(bing)箱、室(shi)溫、37℃水浴逐(zhu)步規則(ze)搖晃均(jun)勻(yun)(yun)融解(jie)，直至(zhi)(zhi)完全(quan)融解(jie)后(hou)(hou)再使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。4、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱滅(mie)活：將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)嚴(yan)格56℃水浴均(jun)勻(yun)(yun)加(jia)溫30分(fen)(fen)(fen)鐘可(ke)(ke)(ke)(ke)(ke)以(yi)(yi)滅(mie)活血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補(bu)體(ti)系統(tong)。是(shi)(shi)(shi)(shi)(shi)不(bu)是(shi)(shi)(shi)(shi)(shi)所有(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)途的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都需(xu)要(yao)(yao)(yao)熱滅(mie)活呢？對(dui)(dui)大(da)多(duo)(duo)數(shu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)來(lai)說是(shi)(shi)(shi)(shi)(shi)不(bu)需(xu)要(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。激(ji)活的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補(bu)體(ti)能(neng)參與(yu)溶解(jie)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)，刺(ci)激(ji)光(guang)滑(hua)肌(ji)收縮(suo)，細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)和(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)板(ban)釋(shi)放(fang)組(zu)胺，激(ji)活淋巴細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)和(he)(he)巨噬(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)。因(yin)(yin)此(ci)在(zai)(zai)(zai)(zai)(zai)免(mian)疫(yi)學研(yan)究，培(pei)養(yang)ES細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)，昆蟲細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)和(he)(he)平滑(hua)肌(ji)肉細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)時，建議使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)熱滅(mie)活血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，在(zai)(zai)(zai)(zai)(zai)正常(chang)情況下(xia)(xia)(xia)(xia)(xia)(xia)加(jia)熱處(chu)(chu)理對(dui)(dui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)促(cu)(cu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)長(chang)效應(ying)無(wu)(wu)(wu)明顯促(cu)(cu)進(jin)(jin)(jin)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，甚(shen)至(zhi)(zhi)會(hui)(hui)因(yin)(yin)為(wei)(wei)(wei)加(jia)熱處(chu)(chu)理影(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)量(liang)(liang)(liang)。5、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)“小(xiao)(xiao)黑點”現(xian)(xian)象：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)(zai)經過(guo)(guo)一(yi)(yi)(yi)段(duan)時間的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低(di)溫凍(dong)存(cun)再融解(jie)后(hou)(hou)，在(zai)(zai)(zai)(zai)(zai)正常(chang)情況下(xia)(xia)(xia)(xia)(xia)(xia)會(hui)(hui)出(chu)現(xian)(xian)絮狀蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)沉(chen)淀(dian)(dian)。但(dan)往(wang)往(wang)有(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)反(fan)應(ying)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)“小(xiao)(xiao)黑點”現(xian)(xian)象，認為(wei)(wei)(wei)是(shi)(shi)(shi)(shi)(shi)微生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)污(wu)染。實(shi)驗(yan)發現(xian)(xian)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)(zai)37℃環境放(fang)置時間過(guo)(guo)長(chang)或經過(guo)(guo)加(jia)熱滅(mie)活處(chu)(chu)理，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)沉(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)往(wang)往(wang)會(hui)(hui)呈增多(duo)(duo)趨(qu)勢(shi)，這(zhe)(zhe)些沉(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)在(zai)(zai)(zai)(zai)(zai)倒置顯微鏡下(xia)(xia)(xia)(xia)(xia)(xia)觀察，像是(shi)(shi)(shi)(shi)(shi)“小(xiao)(xiao)黑點”，讓(rang)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)者誤(wu)以(yi)(yi)為(wei)(wei)(wei)是(shi)(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)遭受微生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)污(wu)染并(bing)(bing)在(zai)(zai)(zai)(zai)(zai)分(fen)(fen)(fen)裂(lie)增殖。因(yin)(yin)此(ci)如(ru)無(wu)(wu)(wu)必(bi)要(yao)(yao)(yao)，完全(quan)可(ke)(ke)(ke)(ke)(ke)以(yi)(yi)不(bu)進(jin)(jin)(jin)行(xing)熱滅(mie)活處(chu)(chu)理并(bing)(bing)保(bao)(bao)(bao)證血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低(di)溫保(bao)(bao)(bao)存(cun)。實(shi)驗(yan)證明使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時注(zhu)(zhu)意(yi)(yi)以(yi)(yi)下(xia)(xia)(xia)(xia)(xia)(xia)幾(ji)點事(shi)項(xiang)不(bu)但(dan)可(ke)(ke)(ke)(ke)(ke)以(yi)(yi)保(bao)(bao)(bao)證血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)量(liang)(liang)(liang)并(bing)(bing)能(neng)給使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)者帶(dai)(dai)來(lai)方(fang)便：◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)(ke)(ke)(ke)在(zai)(zai)(zai)(zai)(zai)-15℃以(yi)(yi)下(xia)(xia)(xia)(xia)(xia)(xia)低(di)溫保(bao)(bao)(bao)存(cun)。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)(yi)(yi)瓶(ping)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)(yi)(yi)次(ci)(ci)無(wu)(wu)(wu)法(fa)(fa)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)分(fen)(fen)(fen)裝凍(dong)存(cun)，分(fen)(fen)(fen)次(ci)(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)按-15℃以(yi)(yi)下(xia)(xia)(xia)(xia)(xia)(xia)至(zhi)(zhi)4℃至(zhi)(zhi)室(shi)溫至(zhi)(zhi)37℃逐(zhu)步均(jun)勻(yun)(yun)解(jie)凍(dong)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。◎若(ruo)非必(bi)要(yao)(yao)(yao)，無(wu)(wu)(wu)須進(jin)(jin)(jin)行(xing)熱滅(mie)活。若(ruo)必(bi)須做血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)熱滅(mie)活的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，須嚴(yan)格控制(zhi)在(zai)(zai)(zai)(zai)(zai)56℃水浴30分(fen)(fen)(fen)鐘均(jun)勻(yun)(yun)加(jia)熱滅(mie)活。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)定(ding)義(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)指血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)除去(qu)(qu)纖維蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)后(hou)(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠(jiao)狀液(ye)(ye)體(ti)。每(mei)100ml人血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)含(han)(han)(han)有(you)(you)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)6-8g，其(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)主(zhu)(zhu)要(yao)(yao)(yao)是(shi)(shi)(shi)(shi)(shi)白(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)和(he)(he)球(qiu)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)不(bu)會(hui)(hui)凝固。有(you)(you)免(mian)疫(yi)，維持酸(suan)堿平衡和(he)(he)滲透壓(ya)(ya)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)亦可(ke)(ke)(ke)(ke)(ke)儲備供給機體(ti)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)不(bu)足(zu)時的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。人和(he)(he)動物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)常(chang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)以(yi)(yi)進(jin)(jin)(jin)行(xing)各種(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)學試驗(yan)，幫(bang)助診斷(duan)疾(ji)病。含(han)(han)(han)抗體(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)(ke)(ke)(ke)作(zuo)(zuo)為(wei)(wei)(wei)預防或治療疾(ji)病之(zhi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)要(yao)(yao)(yao)成(cheng)(cheng)(cheng)分(fen)(fen)(fen)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)由血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)去(qu)(qu)除纖維蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)而形成(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)種(zhong)很(hen)復雜(za)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混(hun)合(he)物(wu)(wu)(wu)(wu)，其(qi)(qi)組(zu)成(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)份(fen)雖(sui)大(da)部(bu)(bu)(bu)(bu)分(fen)(fen)(fen)已知，但(dan)還有(you)(you)一(yi)(yi)(yi)部(bu)(bu)(bu)(bu)分(fen)(fen)(fen)尚(shang)不(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚(chu)(chu)，且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組(zu)成(cheng)(cheng)(cheng)及含(han)(han)(han)量(liang)(liang)(liang)常(chang)隨(sui)供血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)別(bie)、年齡(ling)、生(sheng)(sheng)(sheng)理條(tiao)件(jian)(jian)和(he)(he)營養(yang)條(tiao)件(jian)(jian)不(bu)同而異。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)不(bu)含(han)(han)(han)纖維蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)原(yuan)(yuan)(yuan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠(jiao)狀液(ye)(ye)體(ti),具有(you)(you)維持血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正常(chang)粘度(du)(du)、酸(suan)堿度(du)(du)、滲透壓(ya)(ya)等作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。它(ta)主(zhu)(zhu)要(yao)(yao)(yao)由水和(he)(he)各種(zhong)化(hua)(hua)(hua)學成(cheng)(cheng)(cheng)分(fen)(fen)(fen)組(zu)成(cheng)(cheng)(cheng)，這(zhe)(zhe)些化(hua)(hua)(hua)學成(cheng)(cheng)(cheng)分(fen)(fen)(fen)包(bao)括白(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)，α1、α2、β、γ-球(qiu)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)，甘油(you)三酯，總(zong)膽固醇，谷丙轉(zhuan)氨酶等。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)含(han)(han)(han)有(you)(you)各種(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)蛋(dan)(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)、多(duo)(duo)肽、脂(zhi)肪、碳水化(hua)(hua)(hua)合(he)物(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)長(chang)因(yin)(yin)子、激(ji)素(su)、無(wu)(wu)(wu)機物(wu)(wu)(wu)(wu)等，這(zhe)(zhe)些物(wu)(wu)(wu)(wu)質(zhi)對(dui)(dui)促(cu)(cu)進(jin)(jin)(jin)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)長(chang)或抑制(zhi)生(sheng)(sheng)(sheng)長(chang)活性(xing)是(shi)(shi)(shi)(shi)(shi)達到(dao)生(sheng)(sheng)(sheng)理平衡的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。對(dui)(dui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)分(fen)(fen)(fen)和(he)(he)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)研(yan)究雖(sui)有(you)(you)很(hen)大(da)進(jin)(jin)(jin)展，但(dan)仍存(cun)在(zai)(zai)(zai)(zai)(zai)一(yi)(yi)(yi)些問題。主(zhu)(zhu)要(yao)(yao)(yao)是(shi)(shi)(shi)(shi)(shi)：第(di)一(yi)(yi)(yi)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)份(fen)可(ke)(ke)(ke)(ke)(ke)能(neng)有(you)(you)幾(ji)百種(zhong)之(zhi)多(duo)(duo)，對(dui)(dui)其(qi)(qi)準確的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)份(fen)、含(han)(han)(han)量(liang)(liang)(liang)及其(qi)(qi)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)機制(zhi)仍不(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚(chu)(chu)，尤其(qi)(qi)是(shi)(shi)(shi)(shi)(shi)對(dui)(dui)其(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)一(yi)(yi)(yi)些多(duo)(duo)肽類生(sheng)(sheng)(sheng)長(chang)因(yin)(yin)子、激(ji)素(su)和(he)(he)脂(zhi)類等尚(shang)未充(chong)分(fen)(fen)(fen)認識(shi)，這(zhe)(zhe)給研(yan)究工作(zuo)(zuo)帶(dai)(dai)來(lai)許多(duo)(duo)困(kun)難；第(di)二：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都是(shi)(shi)(shi)(shi)(shi)批量(liang)(liang)(liang)生(sheng)(sheng)(sheng)產(chan)，各批量(liang)(liang)(liang)之(zhi)間差(cha)異很(hen)大(da)，而且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)保(bao)(bao)(bao)存(cun)期至(zhi)(zhi)多(duo)(duo)一(yi)(yi)(yi)年，因(yin)(yin)此(ci)，要(yao)(yao)(yao)保(bao)(bao)(bao)證每(mei)批血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)相似(si)性(xing)極為(wei)(wei)(wei)困(kun)難，從而使(shi)(shi)(shi)實(shi)驗(yan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)準化(hua)(hua)(hua)和(he)(he)連續性(xing)受到(dao)限制(zhi)；第(di)三：不(bu)能(neng)排除血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)含(han)(han)(han)有(you)(you)易變物(wu)(wu)(wu)(wu)質(zhi)，這(zhe)(zhe)被(bei)認為(wei)(wei)(wei)是(shi)(shi)(shi)(shi)(shi)“瓶(ping)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)惡化(hua)(hua)(hua)”的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)(yuan)(yuan)因(yin)(yin)之(zhi)一(yi)(yi)(yi)
小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿（去(qu)紅細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)、無(wu)(wu)菌(jun)(jun)過(guo)濾(lv)）血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)注(zhu)意事項：防(fang)止(zhi)(zhi)發病(bing)免(mian)疫(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)多(duo)(duo)(duo)(duo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)馬、牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)制(zhi)備，馬、牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)對其(qi)(qi)它動(dong)物(wu)(wu)來(lai)說，也具有(you)(you)(you)(you)(you)抗原性(xing)，有(you)(you)(you)(you)(you)引(yin)起(qi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病(bing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)可(ke)(ke)能(neng)，因(yin)(yin)此，使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)免(mian)疫(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)要(yao)(yao)(yao)(yao)(yao)注(zhu)意防(fang)止(zhi)(zhi)引(yin)起(qi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病(bing)，預(yu)防(fang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)(yao)(yao)(yao)(yao)措施是(shi)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)提純(chun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)品(pin)(pin)，不(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)合(he)格的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產品(pin)(pin)；另外，不(bu)(bu)要(yao)(yao)(yao)(yao)(yao)有(you)(you)(you)(you)(you)急功近利的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)想(xiang)法(fa)，要(yao)(yao)(yao)(yao)(yao)按照(zhao)要(yao)(yao)(yao)(yao)(yao)求(qiu)劑(ji)量(liang)(liang)(liang)(liang)(liang)(liang)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，一(yi)(yi)次(ci)(ci)(ci)(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)量(liang)(liang)(liang)(liang)(liang)(liang)不(bu)(bu)可(ke)(ke)過(guo)大(da)(da)(da)(da)。大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)取血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)操(cao)作步(bu)驟：1.剪(jian)尾(wei)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue) 小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)每(mei)次(ci)(ci)(ci)(ci)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)(liang)0.1ml，大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)每(mei)次(ci)(ci)(ci)(ci)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)(liang)0.3-0.5ml左(zuo)(zuo)手拇指和(he)(he)(he)食指從背部(bu)(bu)(bu)(bu)(bu)捉住(zhu)鼠(shu)(shu)(shu)(shu)(shu)(shu)頸部(bu)(bu)(bu)(bu)(bu)皮(pi)膚，將(jiang)鼠(shu)(shu)(shu)(shu)(shu)(shu)頭(tou)朝下(xia)(xia)，鼠(shu)(shu)(shu)(shu)(shu)(shu)保(bao)定后將(jiang)其(qi)(qi)尾(wei)巴置于50℃熱(re)水(shui)中浸(jin)泡數(shu)分(fen)(fen)鐘，使尾(wei)部(bu)(bu)(bu)(bu)(bu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管(guan)充盈(ying)。擦干尾(wei)部(bu)(bu)(bu)(bu)(bu)，再用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)剪(jian)刀(dao)或刀(dao)片剪(jian)去(qu)尾(wei)尖1-2mm，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)試管(guan)接(jie)(jie)流出(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)，同時(shi)(shi)自尾(wei)根(gen)部(bu)(bu)(bu)(bu)(bu)向(xiang)(xiang)尾(wei)尖按-摩。取血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)棉(mian)球(qiu)(qiu)壓(ya)(ya)(ya)榨(zha)止(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)并(bing)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)6%液(ye)體(ti)(ti)火(huo)棉(mian)膠(jiao)涂在(zai)(zai)(zai)傷口處(chu)(chu)(chu)(chu)止(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。 2.去(qu)除眼球(qiu)(qiu)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)(liang)0.5-1ml左(zuo)(zuo)手捉住(zhu)小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)頸部(bu)(bu)(bu)(bu)(bu)皮(pi)膚，輕(qing)壓(ya)(ya)(ya)在(zai)(zai)(zai)實(shi)(shi)驗(yan)臺上，取側(ce)臥位，左(zuo)(zuo)手食指盡量(liang)(liang)(liang)(liang)(liang)(liang)將(jiang)小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)眼周皮(pi)膚往(wang)頸后壓(ya)(ya)(ya)，使眼球(qiu)(qiu)杰出(chu)(chu)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)眼科彎鑷迅速夾去(qu)眼球(qiu)(qiu)，將(jiang)鼠(shu)(shu)(shu)(shu)(shu)(shu)倒立，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)器皿(min)接(jie)(jie)住(zhu)流出(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)。采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)完(wan)(wan)畢立即用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)紗布壓(ya)(ya)(ya)榨(zha)止(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)少用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。 3.心(xin)臟(zang)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)(liang)0.5-0.6ml，大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)(liang)1-1.5ml鼠(shu)(shu)(shu)(shu)(shu)(shu)仰臥位固(gu)(gu)定，剪(jian)去(qu)胸前(qian)區(qu)被毛(mao)(mao)，皮(pi)膚消毒后，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)左(zuo)(zuo)手食指在(zai)(zai)(zai)左(zuo)(zuo)側(ce)第3-4肋間(jian)觸摸(mo)到心(xin)搏(bo)處(chu)(chu)(chu)(chu)，右(you)手持帶有(you)(you)(you)(you)(you)4-5號針頭(tou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)注(zhu)射器，選(xuan)擇心(xin)搏(bo)zui強處(chu)(chu)(chu)(chu)穿(chuan)刺(ci)(ci)，當刺(ci)(ci)中心(xin)臟(zang)時(shi)(shi)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)會(hui)(hui)(hui)主動(dong)進(jin)(jin)(jin)入(ru)(ru)注(zhu)射器。 4.斷(duan)(duan)頭(tou)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.8-1.2ml，大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)(liang)5-10ml左(zuo)(zuo)手拇指和(he)(he)(he)食指從背部(bu)(bu)(bu)(bu)(bu)捉住(zhu)鼠(shu)(shu)(shu)(shu)(shu)(shu)頸部(bu)(bu)(bu)(bu)(bu)皮(pi)膚，將(jiang)鼠(shu)(shu)(shu)(shu)(shu)(shu)頭(tou)朝下(xia)(xia)，右(you)手用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)剪(jian)刀(dao)剪(jian)斷(duan)(duan)鼠(shu)(shu)(shu)(shu)(shu)(shu)頸部(bu)(bu)(bu)(bu)(bu)約1/2-4/5，讓血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)流入(ru)(ru)試管(guan)。 5.眼眶靜脈叢采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)(liang)為(wei)(wei)(wei)0.2-0.4ml，大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)(liang)為(wei)(wei)(wei)0.4-0.8ml取內徑為(wei)(wei)(wei)1.0-1.5mm的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)玻璃毛(mao)(mao)細(xi)(xi)(xi)(xi)管(guan)，臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前(qian)折(zhe)斷(duan)(duan)成(cheng)(cheng)(cheng)(cheng)(cheng)1～1.5cm長(chang)(chang)(chang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)毛(mao)(mao)細(xi)(xi)(xi)(xi)管(guan)段(duan)，浸(jin)入(ru)(ru)1％肝素溶(rong)液(ye)中，干燥后用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。取血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)時(shi)(shi)左(zuo)(zuo)手捉住(zhu)鼠(shu)(shu)(shu)(shu)(shu)(shu)兩耳(er)之(zhi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)頸背部(bu)(bu)(bu)(bu)(bu)皮(pi)膚以(yi)(yi)固(gu)(gu)定頭(tou)部(bu)(bu)(bu)(bu)(bu)，悄(qiao)(qiao)悄(qiao)(qiao)向(xiang)(xiang)下(xia)(xia)壓(ya)(ya)(ya)榨(zha)頸部(bu)(bu)(bu)(bu)(bu)兩邊，引(yin)起(qi)頭(tou)部(bu)(bu)(bu)(bu)(bu)靜脈血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)回流困難(nan)使眼眶靜脈叢充血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)，右(you)手持毛(mao)(mao)細(xi)(xi)(xi)(xi)管(guan)由(you)內眥部(bu)(bu)(bu)(bu)(bu)刺(ci)(ci)進(jin)(jin)(jin)結膜，再悄(qiao)(qiao)悄(qiao)(qiao)向(xiang)(xiang)眼底部(bu)(bu)(bu)(bu)(bu)方(fang)向(xiang)(xiang)推進(jin)(jin)(jin)，悄(qiao)(qiao)悄(qiao)(qiao)旋(xuan)轉(zhuan)毛(mao)(mao)細(xi)(xi)(xi)(xi)管(guan)以(yi)(yi)劃破靜脈叢，讓血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)順毛(mao)(mao)細(xi)(xi)(xi)(xi)管(guan)流出(chu)(chu)，接(jie)(jie)收入(ru)(ru)事前(qian)準(zhun)備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)容(rong)器中。采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后紗布輕(qing)壓(ya)(ya)(ya)眼部(bu)(bu)(bu)(bu)(bu)止(zhi)(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)、大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)、豚鼠(shu)(shu)(shu)(shu)(shu)(shu)及家兔(tu)均(jun)可(ke)(ke)采(cai)(cai)(cai)(cai)納(na)此法(fa)取血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。刺(ci)(ci)入(ru)(ru)深度小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)為(wei)(wei)(wei)2-3mm，可(ke)(ke)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.2-0.3ml；大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)(shu)(shu)為(wei)(wei)(wei)4-5mm，可(ke)(ke)采(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.4-0.8ml。Tips：1. 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)存(cun)：在(zai)(zai)(zai)-15℃以(yi)(yi)下(xia)(xia)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)(you)(you)(you)效(xiao)期(qi)為(wei)(wei)(wei)5年，4℃可(ke)(ke)保(bao)存(cun)數(shu)周，不(bu)(bu)宜(yi)室溫(wen)保(bao)存(cun)，不(bu)(bu)宜(yi)反復(fu)凍(dong)(dong)融。配制(zhi)成(cheng)(cheng)(cheng)(cheng)(cheng)完(wan)(wan)全(quan)培養(yang)基后應(ying)在(zai)(zai)(zai)四周內用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)，一(yi)(yi)瓶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)如(ru)無(wu)(wu)法(fa)一(yi)(yi)次(ci)(ci)(ci)(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)應(ying)無(wu)(wu)菌(jun)(jun)分(fen)(fen)裝(zhuang)成(cheng)(cheng)(cheng)(cheng)(cheng)恰當數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)并(bing)冷凍(dong)(dong)保(bao)存(cun)。2、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)絮狀(zhuang)(zhuang)沉(chen)淀(dian)(dian)物(wu)(wu)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)多(duo)(duo)(duo)(duo)種(zhong)蛋(dan)(dan)白(bai)(bai)(bai)質(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)物(wu)(wu)，在(zai)(zai)(zai)凍(dong)(dong)融過(guo)程中會(hui)(hui)(hui)出(chu)(chu)現(xian)(xian)絮狀(zhuang)(zhuang)沉(chen)淀(dian)(dian)物(wu)(wu)，主要(yao)(yao)(yao)(yao)(yao)原因(yin)(yin)是(shi)由(you)于血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中脂蛋(dan)(dan)白(bai)(bai)(bai)和(he)(he)(he)纖(xian)維(wei)(wei)蛋(dan)(dan)白(bai)(bai)(bai)變性(xing)所(suo)造成(cheng)(cheng)(cheng)(cheng)(cheng)。但并(bing)不(bu)(bu)影(ying)(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本身的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。如(ru)需去(qu)除可(ke)(ke)先(xian)400g離心(xin)取上清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)配制(zhi)成(cheng)(cheng)(cheng)(cheng)(cheng)完(wan)(wan)全(quan)培養(yang)基再過(guo)濾(lv)即可(ke)(ke)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。3、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)(jie)凍(dong)(dong)：合(he)理(li)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)(jie)凍(dong)(dong)方(fang)式可(ke)(ke)以(yi)(yi)更(geng)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)護血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)并(bing)可(ke)(ke)以(yi)(yi)在(zai)(zai)(zai)＊大(da)(da)(da)(da)程度上減少絮狀(zhuang)(zhuang)沉(chen)淀(dian)(dian)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從低(di)溫(wen)冰(bing)箱(xiang)中取出(chu)(chu)后，經(jing)(jing)4℃冰(bing)箱(xiang)、室溫(wen)、37℃水(shui)浴逐步(bu)規則搖晃均(jun)勻融解(jie)(jie)，直至(zhi)(zhi)完(wan)(wan)全(quan)融解(jie)(jie)后再使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。4、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱(re)滅(mie)活(huo)：將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)嚴格56℃水(shui)浴均(jun)勻加溫(wen)30分(fen)(fen)鐘可(ke)(ke)以(yi)(yi)滅(mie)活(huo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補(bu)體(ti)(ti)系統。是(shi)不(bu)(bu)是(shi)所(suo)有(you)(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)途的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都(dou)需要(yao)(yao)(yao)(yao)(yao)熱(re)滅(mie)活(huo)呢？對大(da)(da)(da)(da)多(duo)(duo)(duo)(duo)數(shu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)來(lai)說是(shi)不(bu)(bu)需要(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。激(ji)(ji)活(huo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補(bu)體(ti)(ti)能(neng)參與溶(rong)解(jie)(jie)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，刺(ci)(ci)激(ji)(ji)光滑(hua)肌(ji)收縮，細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)和(he)(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)(xiao)板釋(shi)放(fang)組(zu)胺，激(ji)(ji)活(huo)淋巴細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)和(he)(he)(he)巨噬細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)。因(yin)(yin)此在(zai)(zai)(zai)免(mian)疫(yi)學研(yan)究，培養(yang)ES細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，昆蟲細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)和(he)(he)(he)平(ping)滑(hua)肌(ji)肉(rou)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)時(shi)(shi)，建(jian)議(yi)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)熱(re)滅(mie)活(huo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，在(zai)(zai)(zai)正(zheng)常(chang)(chang)情況下(xia)(xia)加熱(re)處(chu)(chu)(chu)(chu)理(li)對血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)促細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)效(xiao)應(ying)無(wu)(wu)明顯(xian)促進(jin)(jin)(jin)作用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，甚至(zhi)(zhi)會(hui)(hui)(hui)因(yin)(yin)為(wei)(wei)(wei)加熱(re)處(chu)(chu)(chu)(chu)理(li)影(ying)(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)。5、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)“小(xiao)(xiao)(xiao)黑(hei)點(dian)”現(xian)(xian)象：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)經(jing)(jing)過(guo)一(yi)(yi)段(duan)時(shi)(shi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低(di)溫(wen)凍(dong)(dong)存(cun)再融解(jie)(jie)后，在(zai)(zai)(zai)正(zheng)常(chang)(chang)情況下(xia)(xia)會(hui)(hui)(hui)出(chu)(chu)現(xian)(xian)絮狀(zhuang)(zhuang)蛋(dan)(dan)白(bai)(bai)(bai)沉(chen)淀(dian)(dian)。但往(wang)往(wang)有(you)(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶反應(ying)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中有(you)(you)(you)(you)(you)“小(xiao)(xiao)(xiao)黑(hei)點(dian)”現(xian)(xian)象，認(ren)為(wei)(wei)(wei)是(shi)微(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)污染(ran)。實(shi)(shi)驗(yan)發現(xian)(xian)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)37℃環(huan)境(jing)放(fang)置時(shi)(shi)間(jian)過(guo)長(chang)(chang)(chang)或經(jing)(jing)過(guo)加熱(re)滅(mie)活(huo)處(chu)(chu)(chu)(chu)理(li)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)沉(chen)淀(dian)(dian)物(wu)(wu)往(wang)往(wang)會(hui)(hui)(hui)呈增(zeng)多(duo)(duo)(duo)(duo)趨勢，這些(xie)沉(chen)淀(dian)(dian)物(wu)(wu)在(zai)(zai)(zai)倒置顯(xian)微(wei)(wei)鏡下(xia)(xia)觀(guan)察，像是(shi)“小(xiao)(xiao)(xiao)黑(hei)點(dian)”，讓使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)者(zhe)誤以(yi)(yi)為(wei)(wei)(wei)是(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)遭受(shou)微(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)污染(ran)并(bing)在(zai)(zai)(zai)分(fen)(fen)裂增(zeng)殖(zhi)。因(yin)(yin)此如(ru)無(wu)(wu)必(bi)要(yao)(yao)(yao)(yao)(yao)，完(wan)(wan)全(quan)可(ke)(ke)以(yi)(yi)不(bu)(bu)進(jin)(jin)(jin)行熱(re)滅(mie)活(huo)處(chu)(chu)(chu)(chu)理(li)并(bing)保(bao)證血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低(di)溫(wen)保(bao)存(cun)。小(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)(shu)(shu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿（去(qu)紅細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)、無(wu)(wu)菌(jun)(jun)過(guo)濾(lv)）實(shi)(shi)驗(yan)證明使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)注(zhu)意以(yi)(yi)下(xia)(xia)幾點(dian)事項不(bu)(bu)但可(ke)(ke)以(yi)(yi)保(bao)證血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)并(bing)能(neng)給使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)者(zhe)帶來(lai)方(fang)便：◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)在(zai)(zai)(zai)-15℃以(yi)(yi)下(xia)(xia)低(di)溫(wen)保(bao)存(cun)。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)(yi)瓶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)(yi)次(ci)(ci)(ci)(ci)無(wu)(wu)法(fa)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)分(fen)(fen)裝(zhuang)凍(dong)(dong)存(cun)，分(fen)(fen)次(ci)(ci)(ci)(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)按-15℃以(yi)(yi)下(xia)(xia)至(zhi)(zhi)4℃至(zhi)(zhi)室溫(wen)至(zhi)(zhi)37℃逐步(bu)均(jun)勻解(jie)(jie)凍(dong)(dong)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。◎若非必(bi)要(yao)(yao)(yao)(yao)(yao)，無(wu)(wu)須進(jin)(jin)(jin)行熱(re)滅(mie)活(huo)。若必(bi)須做血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)熱(re)滅(mie)活(huo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，須嚴格控(kong)制(zhi)在(zai)(zai)(zai)56℃水(shui)浴30分(fen)(fen)鐘均(jun)勻加熱(re)滅(mie)活(huo)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)定義血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)指血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿除去(qu)纖(xian)維(wei)(wei)蛋(dan)(dan)白(bai)(bai)(bai)原后的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠(jiao)狀(zhuang)(zhuang)液(ye)體(ti)(ti)。每(mei)100ml人血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)含(han)有(you)(you)(you)(you)(you)蛋(dan)(dan)白(bai)(bai)(bai)質(zhi)(zhi)(zhi)6-8g，其(qi)(qi)中主要(yao)(yao)(yao)(yao)(yao)是(shi)白(bai)(bai)(bai)蛋(dan)(dan)白(bai)(bai)(bai)和(he)(he)(he)球(qiu)(qiu)蛋(dan)(dan)白(bai)(bai)(bai)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)不(bu)(bu)會(hui)(hui)(hui)凝固(gu)(gu)。有(you)(you)(you)(you)(you)免(mian)疫(yi)，維(wei)(wei)持酸堿平(ping)衡和(he)(he)(he)滲透壓(ya)(ya)(ya)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)蛋(dan)(dan)白(bai)(bai)(bai)質(zhi)(zhi)(zhi)亦可(ke)(ke)儲備供(gong)給機(ji)體(ti)(ti)蛋(dan)(dan)白(bai)(bai)(bai)質(zhi)(zhi)(zhi)不(bu)(bu)足時(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。人和(he)(he)(he)動(dong)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)常(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)以(yi)(yi)進(jin)(jin)(jin)行各(ge)種(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)學試驗(yan)，幫(bang)助診斷(duan)(duan)疾病(bing)。含(han)抗體(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)作為(wei)(wei)(wei)預(yu)防(fang)或治療(liao)疾病(bing)之(zhi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)(yao)(yao)(yao)(yao)成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)由(you)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿去(qu)除纖(xian)維(wei)(wei)蛋(dan)(dan)白(bai)(bai)(bai)原而(er)形成(cheng)(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)種(zhong)很(hen)復(fu)雜的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)物(wu)(wu)，其(qi)(qi)組(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)雖大(da)(da)(da)(da)部(bu)(bu)(bu)(bu)(bu)分(fen)(fen)已知，但還有(you)(you)(you)(you)(you)一(yi)(yi)部(bu)(bu)(bu)(bu)(bu)分(fen)(fen)尚不(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚(chu)(chu)，且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)及含(han)量(liang)(liang)(liang)(liang)(liang)(liang)常(chang)(chang)隨供(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)物(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)別、年齡、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)條件(jian)和(he)(he)(he)營養(yang)條件(jian)不(bu)(bu)同而(er)異。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿中不(bu)(bu)含(han)纖(xian)維(wei)(wei)蛋(dan)(dan)白(bai)(bai)(bai)原的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠(jiao)狀(zhuang)(zhuang)液(ye)體(ti)(ti),具有(you)(you)(you)(you)(you)維(wei)(wei)持血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正(zheng)常(chang)(chang)粘(zhan)度、酸堿度、滲透壓(ya)(ya)(ya)等(deng)(deng)作用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。它主要(yao)(yao)(yao)(yao)(yao)由(you)水(shui)和(he)(he)(he)各(ge)種(zhong)化(hua)(hua)學成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)組(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)，這些(xie)化(hua)(hua)學成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)包括白(bai)(bai)(bai)蛋(dan)(dan)白(bai)(bai)(bai)，α1、α2、β、γ-球(qiu)(qiu)蛋(dan)(dan)白(bai)(bai)(bai)，甘油三(san)酯，總膽(dan)固(gu)(gu)醇，谷丙轉(zhuan)氨酶等(deng)(deng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中含(han)有(you)(you)(you)(you)(you)各(ge)種(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿蛋(dan)(dan)白(bai)(bai)(bai)、多(duo)(duo)(duo)(duo)肽、脂肪、碳水(shui)化(hua)(hua)合(he)物(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)子(zi)、激(ji)(ji)素、無(wu)(wu)機(ji)物(wu)(wu)等(deng)(deng)，這些(xie)物(wu)(wu)質(zhi)(zhi)(zhi)對促進(jin)(jin)(jin)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)或抑制(zhi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)活(huo)性(xing)是(shi)達到生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)平(ping)衡的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。對血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)和(he)(he)(he)作用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)研(yan)究雖有(you)(you)(you)(you)(you)很(hen)大(da)(da)(da)(da)進(jin)(jin)(jin)展，但仍存(cun)在(zai)(zai)(zai)一(yi)(yi)些(xie)問題。主要(yao)(yao)(yao)(yao)(yao)是(shi)：第一(yi)(yi)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)可(ke)(ke)能(neng)有(you)(you)(you)(you)(you)幾百種(zhong)之(zhi)多(duo)(duo)(duo)(duo)，對其(qi)(qi)準(zhun)確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)、含(han)量(liang)(liang)(liang)(liang)(liang)(liang)及其(qi)(qi)作用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)機(ji)制(zhi)仍不(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚(chu)(chu)，尤其(qi)(qi)是(shi)對其(qi)(qi)中一(yi)(yi)些(xie)多(duo)(duo)(duo)(duo)肽類生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)(chang)(chang)因(yin)(yin)子(zi)、激(ji)(ji)素和(he)(he)(he)脂類等(deng)(deng)尚未充分(fen)(fen)認(ren)識，這給研(yan)究工作帶來(lai)許多(duo)(duo)(duo)(duo)困難(nan)；第二：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都(dou)是(shi)批量(liang)(liang)(liang)(liang)(liang)(liang)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產，各(ge)批量(liang)(liang)(liang)(liang)(liang)(liang)之(zhi)間(jian)差異很(hen)大(da)(da)(da)(da)，而(er)且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)保(bao)存(cun)期(qi)至(zhi)(zhi)多(duo)(duo)(duo)(duo)一(yi)(yi)年，因(yin)(yin)此，要(yao)(yao)(yao)(yao)(yao)保(bao)證每(mei)批血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)相似性(xing)極為(wei)(wei)(wei)困難(nan)，從而(er)使實(shi)(shi)驗(yan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標準(zhun)化(hua)(hua)和(he)(he)(he)連續性(xing)受(shou)到限制(zhi)；第三(san)：不(bu)(bu)能(neng)排除血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中含(han)有(you)(you)(you)(you)(you)易(yi)變物(wu)(wu)質(zhi)(zhi)(zhi)，這被認(ren)為(wei)(wei)(wei)是(shi)“瓶中惡化(hua)(hua)”的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原因(yin)(yin)之(zhi)一(yi)(yi)
大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)（去紅細(xi)(xi)(xi)(xi)胞(bao)、無(wu)菌(jun)過(guo)(guo)濾）血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)注(zhu)(zhu)意(yi)事(shi)項(xiang)：防(fang)(fang)止發(fa)病免(mian)疫血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)多(duo)(duo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)馬(ma)、牛(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)制(zhi)(zhi)備，馬(ma)、牛(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)對(dui)其(qi)(qi)它動(dong)物(wu)(wu)(wu)(wu)來說，也具有(you)(you)(you)(you)(you)抗(kang)原性(xing)(xing)，有(you)(you)(you)(you)(you)引起(qi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)可(ke)(ke)(ke)能，因(yin)(yin)(yin)此(ci)(ci)，使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)免(mian)疫血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)要(yao)(yao)(yao)(yao)注(zhu)(zhu)意(yi)防(fang)(fang)止引起(qi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病，預防(fang)(fang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要(yao)(yao)(yao)(yao)措施是(shi)(shi)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)提純(chun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)(zhi)品，不(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)合(he)(he)格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)品；另外，不(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)有(you)(you)(you)(you)(you)急功近利的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)想(xiang)法(fa)，要(yao)(yao)(yao)(yao)按(an)照要(yao)(yao)(yao)(yao)求劑(ji)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，一(yi)(yi)(yi)次(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)(bu)可(ke)(ke)(ke)過(guo)(guo)大(da)(da)(da)(da)。大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)小(xiao)鼠(shu)(shu)(shu)(shu)取(qu)(qu)(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)操(cao)作(zuo)步(bu)驟：1.剪(jian)尾(wei)(wei)采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue) 小(xiao)鼠(shu)(shu)(shu)(shu)每(mei)次(ci)采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)0.1ml，大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)每(mei)次(ci)采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)0.3-0.5ml左(zuo)(zuo)(zuo)手(shou)(shou)拇指(zhi)和(he)(he)(he)食指(zhi)從背部(bu)(bu)(bu)捉(zhuo)住鼠(shu)(shu)(shu)(shu)頸(jing)部(bu)(bu)(bu)皮膚(fu)(fu)(fu)，將(jiang)鼠(shu)(shu)(shu)(shu)頭(tou)(tou)朝下(xia)(xia)(xia)，鼠(shu)(shu)(shu)(shu)保(bao)(bao)定(ding)后(hou)將(jiang)其(qi)(qi)尾(wei)(wei)巴(ba)置(zhi)于(yu)50℃熱(re)(re)水(shui)中(zhong)(zhong)(zhong)浸(jin)泡數分(fen)(fen)(fen)(fen)(fen)鐘(zhong)，使(shi)(shi)(shi)(shi)尾(wei)(wei)部(bu)(bu)(bu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管(guan)(guan)(guan)充盈。擦干(gan)尾(wei)(wei)部(bu)(bu)(bu)，再用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)剪(jian)刀(dao)或刀(dao)片剪(jian)去尾(wei)(wei)尖(jian)1-2mm，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)試(shi)管(guan)(guan)(guan)接(jie)流(liu)(liu)出(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)，同時(shi)(shi)(shi)自尾(wei)(wei)根(gen)部(bu)(bu)(bu)向(xiang)尾(wei)(wei)尖(jian)按(an)-摩(mo)。取(qu)(qu)(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后(hou)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)棉球(qiu)(qiu)(qiu)壓(ya)榨(zha)止血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)并(bing)(bing)(bing)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)6%液(ye)(ye)(ye)(ye)體(ti)火(huo)棉膠涂在(zai)(zai)傷(shang)口處(chu)(chu)止血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。 2.去除(chu)眼(yan)(yan)(yan)球(qiu)(qiu)(qiu)采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)鼠(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)0.5-1ml左(zuo)(zuo)(zuo)手(shou)(shou)捉(zhuo)住小(xiao)鼠(shu)(shu)(shu)(shu)頸(jing)部(bu)(bu)(bu)皮膚(fu)(fu)(fu)，輕壓(ya)在(zai)(zai)實(shi)驗(yan)(yan)臺上(shang)，取(qu)(qu)(qu)(qu)側臥位，左(zuo)(zuo)(zuo)手(shou)(shou)食指(zhi)盡量(liang)(liang)(liang)(liang)(liang)(liang)(liang)將(jiang)小(xiao)鼠(shu)(shu)(shu)(shu)眼(yan)(yan)(yan)周(zhou)皮膚(fu)(fu)(fu)往(wang)(wang)頸(jing)后(hou)壓(ya)，使(shi)(shi)(shi)(shi)眼(yan)(yan)(yan)球(qiu)(qiu)(qiu)杰出(chu)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)眼(yan)(yan)(yan)科彎鑷迅速夾去眼(yan)(yan)(yan)球(qiu)(qiu)(qiu)，將(jiang)鼠(shu)(shu)(shu)(shu)倒立(li)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)器(qi)皿接(jie)住流(liu)(liu)出(chu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)。采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)完(wan)(wan)畢立(li)即用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)紗布壓(ya)榨(zha)止血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)少(shao)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。 3.心(xin)臟采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)鼠(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)0.5-0.6ml，大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)1-1.5ml鼠(shu)(shu)(shu)(shu)仰(yang)臥位固(gu)(gu)定(ding)，剪(jian)去胸(xiong)前區被毛(mao)(mao)(mao)，皮膚(fu)(fu)(fu)消毒后(hou)，用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)左(zuo)(zuo)(zuo)手(shou)(shou)食指(zhi)在(zai)(zai)左(zuo)(zuo)(zuo)側第3-4肋間(jian)觸摸到心(xin)搏處(chu)(chu)，右(you)手(shou)(shou)持帶(dai)有(you)(you)(you)(you)(you)4-5號針頭(tou)(tou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)注(zhu)(zhu)射器(qi)，選(xuan)擇心(xin)搏zui強處(chu)(chu)穿刺(ci)，當(dang)刺(ci)中(zhong)(zhong)(zhong)心(xin)臟時(shi)(shi)(shi)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)會(hui)主(zhu)動(dong)進(jin)入(ru)(ru)注(zhu)(zhu)射器(qi)。 4.斷頭(tou)(tou)采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)鼠(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.8-1.2ml，大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)5-10ml左(zuo)(zuo)(zuo)手(shou)(shou)拇指(zhi)和(he)(he)(he)食指(zhi)從背部(bu)(bu)(bu)捉(zhuo)住鼠(shu)(shu)(shu)(shu)頸(jing)部(bu)(bu)(bu)皮膚(fu)(fu)(fu)，將(jiang)鼠(shu)(shu)(shu)(shu)頭(tou)(tou)朝下(xia)(xia)(xia)，右(you)手(shou)(shou)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)剪(jian)刀(dao)剪(jian)斷鼠(shu)(shu)(shu)(shu)頸(jing)部(bu)(bu)(bu)約(yue)1/2-4/5，讓(rang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)流(liu)(liu)入(ru)(ru)試(shi)管(guan)(guan)(guan)。 5.眼(yan)(yan)(yan)眶靜(jing)脈叢(cong)采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)鼠(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)為(wei)(wei)0.2-0.4ml，大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)為(wei)(wei)0.4-0.8ml取(qu)(qu)(qu)(qu)內(nei)徑為(wei)(wei)1.0-1.5mm的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)玻璃毛(mao)(mao)(mao)細(xi)(xi)(xi)(xi)管(guan)(guan)(guan)，臨(lin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)前折斷成(cheng)(cheng)1～1.5cm長(chang)(chang)(chang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)毛(mao)(mao)(mao)細(xi)(xi)(xi)(xi)管(guan)(guan)(guan)段(duan)，浸(jin)入(ru)(ru)1％肝素溶(rong)液(ye)(ye)(ye)(ye)中(zhong)(zhong)(zhong)，干(gan)燥后(hou)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。取(qu)(qu)(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)時(shi)(shi)(shi)左(zuo)(zuo)(zuo)手(shou)(shou)捉(zhuo)住鼠(shu)(shu)(shu)(shu)兩(liang)耳之間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)頸(jing)背部(bu)(bu)(bu)皮膚(fu)(fu)(fu)以(yi)(yi)(yi)固(gu)(gu)定(ding)頭(tou)(tou)部(bu)(bu)(bu)，悄(qiao)(qiao)悄(qiao)(qiao)向(xiang)下(xia)(xia)(xia)壓(ya)榨(zha)頸(jing)部(bu)(bu)(bu)兩(liang)邊(bian)，引起(qi)頭(tou)(tou)部(bu)(bu)(bu)靜(jing)脈血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)回流(liu)(liu)困難使(shi)(shi)(shi)(shi)眼(yan)(yan)(yan)眶靜(jing)脈叢(cong)充血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)，右(you)手(shou)(shou)持毛(mao)(mao)(mao)細(xi)(xi)(xi)(xi)管(guan)(guan)(guan)由內(nei)眥部(bu)(bu)(bu)刺(ci)進(jin)結(jie)膜，再悄(qiao)(qiao)悄(qiao)(qiao)向(xiang)眼(yan)(yan)(yan)底部(bu)(bu)(bu)方向(xiang)推進(jin)，悄(qiao)(qiao)悄(qiao)(qiao)旋(xuan)轉毛(mao)(mao)(mao)細(xi)(xi)(xi)(xi)管(guan)(guan)(guan)以(yi)(yi)(yi)劃破靜(jing)脈叢(cong)，讓(rang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)順毛(mao)(mao)(mao)細(xi)(xi)(xi)(xi)管(guan)(guan)(guan)流(liu)(liu)出(chu)，接(jie)收(shou)入(ru)(ru)事(shi)前準備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)容器(qi)中(zhong)(zhong)(zhong)。采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后(hou)紗布輕壓(ya)眼(yan)(yan)(yan)部(bu)(bu)(bu)止血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。小(xiao)鼠(shu)(shu)(shu)(shu)、大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)、豚鼠(shu)(shu)(shu)(shu)及家兔均可(ke)(ke)(ke)采(cai)(cai)(cai)(cai)(cai)納此(ci)(ci)法(fa)取(qu)(qu)(qu)(qu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)。刺(ci)入(ru)(ru)深度小(xiao)鼠(shu)(shu)(shu)(shu)為(wei)(wei)2-3mm，可(ke)(ke)(ke)采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.2-0.3ml；大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)為(wei)(wei)4-5mm，可(ke)(ke)(ke)采(cai)(cai)(cai)(cai)(cai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)0.4-0.8ml。大(da)(da)(da)(da)鼠(shu)(shu)(shu)(shu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)（去紅細(xi)(xi)(xi)(xi)胞(bao)、無(wu)菌(jun)過(guo)(guo)濾）Tips：1. 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)存(cun)(cun)(cun)：在(zai)(zai)-15℃以(yi)(yi)(yi)下(xia)(xia)(xia)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)(you)(you)(you)效期為(wei)(wei)5年(nian)，4℃可(ke)(ke)(ke)保(bao)(bao)存(cun)(cun)(cun)數周(zhou)，不(bu)(bu)(bu)(bu)(bu)宜室溫保(bao)(bao)存(cun)(cun)(cun)，不(bu)(bu)(bu)(bu)(bu)宜反復(fu)(fu)凍(dong)融(rong)。配制(zhi)(zhi)成(cheng)(cheng)完(wan)(wan)全(quan)培(pei)養基后(hou)應(ying)在(zai)(zai)四周(zhou)內(nei)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)，一(yi)(yi)(yi)瓶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)如無(wu)法(fa)一(yi)(yi)(yi)次(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)應(ying)無(wu)菌(jun)分(fen)(fen)(fen)(fen)(fen)裝成(cheng)(cheng)恰當(dang)數量(liang)(liang)(liang)(liang)(liang)(liang)(liang)并(bing)(bing)(bing)冷(leng)凍(dong)保(bao)(bao)存(cun)(cun)(cun)。2、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)絮狀(zhuang)(zhuang)(zhuang)沉(chen)淀(dian)物(wu)(wu)(wu)(wu)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)多(duo)(duo)種(zhong)(zhong)(zhong)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)(he)物(wu)(wu)(wu)(wu)，在(zai)(zai)凍(dong)融(rong)過(guo)(guo)程中(zhong)(zhong)(zhong)會(hui)出(chu)現絮狀(zhuang)(zhuang)(zhuang)沉(chen)淀(dian)物(wu)(wu)(wu)(wu)，主(zhu)要(yao)(yao)(yao)(yao)原因(yin)(yin)(yin)是(shi)(shi)由于(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)脂(zhi)(zhi)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)和(he)(he)(he)纖維(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)變(bian)性(xing)(xing)所(suo)造成(cheng)(cheng)。但(dan)(dan)并(bing)(bing)(bing)不(bu)(bu)(bu)(bu)(bu)影響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本身的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。如需去除(chu)可(ke)(ke)(ke)先400g離(li)心(xin)取(qu)(qu)(qu)(qu)上(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)配制(zhi)(zhi)成(cheng)(cheng)完(wan)(wan)全(quan)培(pei)養基再過(guo)(guo)濾即可(ke)(ke)(ke)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。3、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)凍(dong)：合(he)(he)理(li)(li)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)凍(dong)方式可(ke)(ke)(ke)以(yi)(yi)(yi)更好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)護血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)并(bing)(bing)(bing)可(ke)(ke)(ke)以(yi)(yi)(yi)在(zai)(zai)＊大(da)(da)(da)(da)程度上(shang)減少(shao)絮狀(zhuang)(zhuang)(zhuang)沉(chen)淀(dian)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)生。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從低(di)溫冰箱中(zhong)(zhong)(zhong)取(qu)(qu)(qu)(qu)出(chu)后(hou)，經4℃冰箱、室溫、37℃水(shui)浴逐(zhu)步(bu)規則搖(yao)晃均勻(yun)融(rong)解(jie)，直至(zhi)完(wan)(wan)全(quan)融(rong)解(jie)后(hou)再使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。4、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱(re)(re)滅(mie)(mie)活(huo)(huo)(huo)(huo)(huo)：將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)嚴格(ge)(ge)56℃水(shui)浴均勻(yun)加溫30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)可(ke)(ke)(ke)以(yi)(yi)(yi)滅(mie)(mie)活(huo)(huo)(huo)(huo)(huo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補體(ti)系統。是(shi)(shi)不(bu)(bu)(bu)(bu)(bu)是(shi)(shi)所(suo)有(you)(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)途(tu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都需要(yao)(yao)(yao)(yao)熱(re)(re)滅(mie)(mie)活(huo)(huo)(huo)(huo)(huo)呢(ni)？對(dui)大(da)(da)(da)(da)多(duo)(duo)數細(xi)(xi)(xi)(xi)胞(bao)來說是(shi)(shi)不(bu)(bu)(bu)(bu)(bu)需要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。激(ji)活(huo)(huo)(huo)(huo)(huo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補體(ti)能參與(yu)溶(rong)解(jie)細(xi)(xi)(xi)(xi)胞(bao)，刺(ci)激(ji)光滑(hua)肌(ji)收(shou)縮(suo)，細(xi)(xi)(xi)(xi)胞(bao)和(he)(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)板釋放組(zu)(zu)(zu)胺，激(ji)活(huo)(huo)(huo)(huo)(huo)淋(lin)巴(ba)細(xi)(xi)(xi)(xi)胞(bao)和(he)(he)(he)巨(ju)噬細(xi)(xi)(xi)(xi)胞(bao)。因(yin)(yin)(yin)此(ci)(ci)在(zai)(zai)免(mian)疫學研究，培(pei)養ES細(xi)(xi)(xi)(xi)胞(bao)，昆蟲細(xi)(xi)(xi)(xi)胞(bao)和(he)(he)(he)平滑(hua)肌(ji)肉細(xi)(xi)(xi)(xi)胞(bao)時(shi)(shi)(shi)，建議(yi)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)熱(re)(re)滅(mie)(mie)活(huo)(huo)(huo)(huo)(huo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，在(zai)(zai)正常(chang)(chang)情(qing)(qing)況下(xia)(xia)(xia)加熱(re)(re)處(chu)(chu)理(li)(li)對(dui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)促(cu)(cu)細(xi)(xi)(xi)(xi)胞(bao)生長(chang)(chang)(chang)效應(ying)無(wu)明(ming)顯(xian)促(cu)(cu)進(jin)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，甚至(zhi)會(hui)因(yin)(yin)(yin)為(wei)(wei)加熱(re)(re)處(chu)(chu)理(li)(li)影響(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)。5、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)“小(xiao)黑(hei)(hei)(hei)點(dian)”現象：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)經過(guo)(guo)一(yi)(yi)(yi)段(duan)時(shi)(shi)(shi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低(di)溫凍(dong)存(cun)(cun)(cun)再融(rong)解(jie)后(hou)，在(zai)(zai)正常(chang)(chang)情(qing)(qing)況下(xia)(xia)(xia)會(hui)出(chu)現絮狀(zhuang)(zhuang)(zhuang)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)沉(chen)淀(dian)。但(dan)(dan)往(wang)(wang)往(wang)(wang)有(you)(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶反應(ying)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)有(you)(you)(you)(you)(you)“小(xiao)黑(hei)(hei)(hei)點(dian)”現象，認(ren)(ren)為(wei)(wei)是(shi)(shi)微(wei)生物(wu)(wu)(wu)(wu)污染(ran)。實(shi)驗(yan)(yan)發(fa)現血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)37℃環境放置(zhi)時(shi)(shi)(shi)間(jian)過(guo)(guo)長(chang)(chang)(chang)或經過(guo)(guo)加熱(re)(re)滅(mie)(mie)活(huo)(huo)(huo)(huo)(huo)處(chu)(chu)理(li)(li)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)沉(chen)淀(dian)物(wu)(wu)(wu)(wu)往(wang)(wang)往(wang)(wang)會(hui)呈增多(duo)(duo)趨勢，這(zhe)些沉(chen)淀(dian)物(wu)(wu)(wu)(wu)在(zai)(zai)倒置(zhi)顯(xian)微(wei)鏡(jing)下(xia)(xia)(xia)觀察，像是(shi)(shi)“小(xiao)黑(hei)(hei)(hei)點(dian)”，讓(rang)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)者誤以(yi)(yi)(yi)為(wei)(wei)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)遭受(shou)微(wei)生物(wu)(wu)(wu)(wu)污染(ran)并(bing)(bing)(bing)在(zai)(zai)分(fen)(fen)(fen)(fen)(fen)裂增殖。因(yin)(yin)(yin)此(ci)(ci)如無(wu)必(bi)要(yao)(yao)(yao)(yao)，完(wan)(wan)全(quan)可(ke)(ke)(ke)以(yi)(yi)(yi)不(bu)(bu)(bu)(bu)(bu)進(jin)行熱(re)(re)滅(mie)(mie)活(huo)(huo)(huo)(huo)(huo)處(chu)(chu)理(li)(li)并(bing)(bing)(bing)保(bao)(bao)證(zheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低(di)溫保(bao)(bao)存(cun)(cun)(cun)。實(shi)驗(yan)(yan)證(zheng)明(ming)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)(shi)注(zhu)(zhu)意(yi)以(yi)(yi)(yi)下(xia)(xia)(xia)幾(ji)點(dian)事(shi)項(xiang)不(bu)(bu)(bu)(bu)(bu)但(dan)(dan)可(ke)(ke)(ke)以(yi)(yi)(yi)保(bao)(bao)證(zheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)并(bing)(bing)(bing)能給(gei)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)者帶(dai)來方便：◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)(ke)在(zai)(zai)-15℃以(yi)(yi)(yi)下(xia)(xia)(xia)低(di)溫保(bao)(bao)存(cun)(cun)(cun)。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)(yi)(yi)瓶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)(yi)(yi)次(ci)無(wu)法(fa)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)分(fen)(fen)(fen)(fen)(fen)裝凍(dong)存(cun)(cun)(cun)，分(fen)(fen)(fen)(fen)(fen)次(ci)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)(wan)。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)按(an)-15℃以(yi)(yi)(yi)下(xia)(xia)(xia)至(zhi)4℃至(zhi)室溫至(zhi)37℃逐(zhu)步(bu)均勻(yun)解(jie)凍(dong)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。◎若非必(bi)要(yao)(yao)(yao)(yao)，無(wu)須(xu)進(jin)行熱(re)(re)滅(mie)(mie)活(huo)(huo)(huo)(huo)(huo)。若必(bi)須(xu)做血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)熱(re)(re)滅(mie)(mie)活(huo)(huo)(huo)(huo)(huo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，須(xu)嚴格(ge)(ge)控(kong)制(zhi)(zhi)在(zai)(zai)56℃水(shui)浴30分(fen)(fen)(fen)(fen)(fen)鐘(zhong)均勻(yun)加熱(re)(re)滅(mie)(mie)活(huo)(huo)(huo)(huo)(huo)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)定(ding)義血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)指(zhi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)除(chu)去纖維(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)原后(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠狀(zhuang)(zhuang)(zhuang)液(ye)(ye)(ye)(ye)體(ti)。每(mei)100ml人(ren)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)含(han)(han)有(you)(you)(you)(you)(you)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)6-8g，其(qi)(qi)中(zhong)(zhong)(zhong)主(zhu)要(yao)(yao)(yao)(yao)是(shi)(shi)白(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)和(he)(he)(he)球(qiu)(qiu)(qiu)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)不(bu)(bu)(bu)(bu)(bu)會(hui)凝(ning)固(gu)(gu)。有(you)(you)(you)(you)(you)免(mian)疫，維(wei)持酸堿平衡(heng)和(he)(he)(he)滲透(tou)壓(ya)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)亦可(ke)(ke)(ke)儲備供(gong)給(gei)機(ji)(ji)體(ti)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)(zhi)不(bu)(bu)(bu)(bu)(bu)足時(shi)(shi)(shi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。人(ren)和(he)(he)(he)動(dong)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)常(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)以(yi)(yi)(yi)進(jin)行各種(zhong)(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)學試(shi)驗(yan)(yan)，幫助(zhu)診斷疾(ji)病。含(han)(han)抗(kang)體(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)(ke)作(zuo)為(wei)(wei)預防(fang)(fang)或治療(liao)疾(ji)病之用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要(yao)(yao)(yao)(yao)成(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)由血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)去除(chu)纖維(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)原而形(xing)成(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)種(zhong)(zhong)(zhong)很(hen)復(fu)(fu)雜的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)(he)物(wu)(wu)(wu)(wu)，其(qi)(qi)組(zu)(zu)(zu)成(cheng)(cheng)成(cheng)(cheng)份雖大(da)(da)(da)(da)部(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)已知，但(dan)(dan)還有(you)(you)(you)(you)(you)一(yi)(yi)(yi)部(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)尚(shang)不(bu)(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組(zu)(zu)(zu)成(cheng)(cheng)及含(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)常(chang)(chang)隨供(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動(dong)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)(xing)別(bie)、年(nian)齡(ling)、生理(li)(li)條件(jian)和(he)(he)(he)營(ying)養條件(jian)不(bu)(bu)(bu)(bu)(bu)同而異。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)中(zhong)(zhong)(zhong)不(bu)(bu)(bu)(bu)(bu)含(han)(han)纖維(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)原的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠狀(zhuang)(zhuang)(zhuang)液(ye)(ye)(ye)(ye)體(ti),具有(you)(you)(you)(you)(you)維(wei)持血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正常(chang)(chang)粘度、酸堿度、滲透(tou)壓(ya)等作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。它主(zhu)要(yao)(yao)(yao)(yao)由水(shui)和(he)(he)(he)各種(zhong)(zhong)(zhong)化學成(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)組(zu)(zu)(zu)成(cheng)(cheng)，這(zhe)些化學成(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)包括白(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)，α1、α2、β、γ-球(qiu)(qiu)(qiu)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)，甘油三酯，總膽固(gu)(gu)醇，谷丙轉氨(an)酶等。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)含(han)(han)有(you)(you)(you)(you)(you)各種(zhong)(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)、多(duo)(duo)肽(tai)、脂(zhi)(zhi)肪、碳水(shui)化合(he)(he)物(wu)(wu)(wu)(wu)、生長(chang)(chang)(chang)因(yin)(yin)(yin)子、激(ji)素、無(wu)機(ji)(ji)物(wu)(wu)(wu)(wu)等，這(zhe)些物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)對(dui)促(cu)(cu)進(jin)細(xi)(xi)(xi)(xi)胞(bao)生長(chang)(chang)(chang)或抑(yi)制(zhi)(zhi)生長(chang)(chang)(chang)活(huo)(huo)(huo)(huo)(huo)性(xing)(xing)是(shi)(shi)達到生理(li)(li)平衡(heng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。對(dui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)和(he)(he)(he)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)研究雖有(you)(you)(you)(you)(you)很(hen)大(da)(da)(da)(da)進(jin)展(zhan)，但(dan)(dan)仍存(cun)(cun)(cun)在(zai)(zai)一(yi)(yi)(yi)些問題。主(zhu)要(yao)(yao)(yao)(yao)是(shi)(shi)：第一(yi)(yi)(yi)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)份可(ke)(ke)(ke)能有(you)(you)(you)(you)(you)幾(ji)百種(zhong)(zhong)(zhong)之多(duo)(duo)，對(dui)其(qi)(qi)準確(que)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)份、含(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)及其(qi)(qi)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)機(ji)(ji)制(zhi)(zhi)仍不(bu)(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，尤其(qi)(qi)是(shi)(shi)對(dui)其(qi)(qi)中(zhong)(zhong)(zhong)一(yi)(yi)(yi)些多(duo)(duo)肽(tai)類生長(chang)(chang)(chang)因(yin)(yin)(yin)子、激(ji)素和(he)(he)(he)脂(zhi)(zhi)類等尚(shang)未充分(fen)(fen)(fen)(fen)(fen)認(ren)(ren)識，這(zhe)給(gei)研究工作(zuo)帶(dai)來許多(duo)(duo)困難；第二：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都是(shi)(shi)批量(liang)(liang)(liang)(liang)(liang)(liang)(liang)生產(chan)，各批量(liang)(liang)(liang)(liang)(liang)(liang)(liang)之間(jian)差(cha)異很(hen)大(da)(da)(da)(da)，而且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)保(bao)(bao)存(cun)(cun)(cun)期至(zhi)多(duo)(duo)一(yi)(yi)(yi)年(nian)，因(yin)(yin)(yin)此(ci)(ci)，要(yao)(yao)(yao)(yao)保(bao)(bao)證(zheng)每(mei)批血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)相似性(xing)(xing)極為(wei)(wei)困難，從而使(shi)(shi)(shi)(shi)實(shi)驗(yan)(yan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標準化和(he)(he)(he)連續性(xing)(xing)受(shou)到限制(zhi)(zhi)；第三：不(bu)(bu)(bu)(bu)(bu)能排(pai)除(chu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)含(han)(han)有(you)(you)(you)(you)(you)易(yi)變(bian)物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)，這(zhe)被認(ren)(ren)為(wei)(wei)是(shi)(shi)“瓶中(zhong)(zhong)(zhong)惡(e)化”的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原因(yin)(yin)(yin)之一(yi)(yi)(yi)
雞(ji)血(xue)(xue)(xue)(xue)漿（去(qu)紅細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)、無(wu)(wu)菌(jun)(jun)(jun)過(guo)(guo)(guo)(guo)濾）適用(yong)(yong)(yong)(yong)(yong)(yong)范圍【產(chan)(chan)(chan)(chan)(chan)品(pin)(pin)(pin)(pin)名稱(cheng)】 雞(ji)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)（無(wu)(wu)菌(jun)(jun)(jun)過(guo)(guo)(guo)(guo)濾）【產(chan)(chan)(chan)(chan)(chan)品(pin)(pin)(pin)(pin)規(gui)格】100ml/200ml/500ml/500ml*20瓶【產(chan)(chan)(chan)(chan)(chan)品(pin)(pin)(pin)(pin)價(jia)格】130元-4500元【適用(yong)(yong)(yong)(yong)(yong)(yong)范圍]適應(ying)于(yu)(yu)(yu)(yu)(yu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)培(pei)(pei)養(yang)、免(mian)(mian)疫(yi)檢(jian)測(ce)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)。【儲存(cun)條件及(ji)有(you)(you)效(xiao)(xiao)期]1、密(mi)封保存(cun)于(yu)(yu)(yu)(yu)(yu)-10℃~-30℃2、有(you)(you)效(xiao)(xiao)期五年[注(zhu)意(yi)事(shi)項]1、本產(chan)(chan)(chan)(chan)(chan)品(pin)(pin)(pin)(pin)僅供(gong)科(ke)(ke)(ke)(ke)研(yan)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)。2、產(chan)(chan)(chan)(chan)(chan)品(pin)(pin)(pin)(pin)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)過(guo)(guo)(guo)(guo)程中(zhong)，注(zhu)意(yi)防止污(wu)染(ran)，在室溫(wen)(wen)(wen)內放置(zhi)(zhi)過(guo)(guo)(guo)(guo)久會影響(xiang)使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)效(xiao)(xiao)果，使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)后(hou)請盡快放入-10℃~-33、請勿(wu)將使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)后(hou)剩余(yu)的(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)劑(ji)(ji)(ji)(ji)再(zai)倒(dao)入原(yuan)包(bao)裝瓶內。【生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)(chan)(chan)日期]詳(xiang)見標(biao)(biao)簽，請在產(chan)(chan)(chan)(chan)(chan)品(pin)(pin)(pin)(pin)標(biao)(biao)簽標(biao)(biao)注(zhu)的(de)(de)(de)(de)(de)(de)(de)有(you)(you)效(xiao)(xiao)期內使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)。產(chan)(chan)(chan)(chan)(chan)品(pin)(pin)(pin)(pin)介紹 雞(ji)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)培(pei)(pei)養(yang)中(zhong)的(de)(de)(de)(de)(de)(de)(de)天(tian)然(ran)(ran)培(pei)(pei)養(yang)基,含有(you)(you)豐富的(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)必(bi)須(xu)的(de)(de)(de)(de)(de)(de)(de)營養(yang)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)份(fen),常用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)動(dong)物(wu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)體(ti)(ti)外培(pei)(pei)養(yang),具(ju)(ju)有(you)(you)極為(wei)(wei)重(zhong)要(yao)的(de)(de)(de)(de)(de)(de)(de)功能。1.提供(gong)對(dui)(dui)維持細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)指(zhi)(zhi)數生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)的(de)(de)(de)(de)(de)(de)(de)激素(su)(su),基礎培(pei)(pei)養(yang)基中(zhong)沒有(you)(you)或(huo)量(liang)(liang)很少的(de)(de)(de)(de)(de)(de)(de)營養(yang)物(wu),以(yi)及(ji)主(zhu)要(yao)的(de)(de)(de)(de)(de)(de)(de)低分(fen)子營養(yang)物(wu)。2. 提供(gong)結合(he)蛋(dan)白(bai),能識別維生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)素(su)(su)、脂類(lei)、金屬(shu)和(he)(he)(he)(he)其(qi)他(ta)激素(su)(su)等(deng)(deng)(deng)(deng)(deng)(deng)(deng),能結合(he)或(huo)調變它們所結合(he)的(de)(de)(de)(de)(de)(de)(de)物(wu)質(zhi)活(huo)力(li)。3.有(you)(you)些(xie)(xie)情況下結合(he)蛋(dan)白(bai)質(zhi)能與(yu)(yu)有(you)(you)毒金屬(shu)和(he)(he)(he)(he)熱原(yuan)質(zhi)結合(he),起(qi)到作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。4.是(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)貼壁(bi)、鋪展在塑料(liao)培(pei)(pei)養(yang)基質(zhi)上(shang)所需(xu)因(yin)(yin)子來源。5.起(qi)酸堿度緩沖液作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。 6.提供(gong)蛋(dan)白(bai)酶抑制(zhi)(zhi)劑(ji)(ji)(ji)(ji),使(shi)在細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)傳(chuan)代(dai)時使(shi)剩余(yu)胰蛋(dan)白(bai)酶失(shi)活(huo),保護細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)不受(shou)傷害(hai)。 雞(ji)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)和(he)(he)(he)(he)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)研(yan)究(jiu)雖有(you)(you)很大(da)進展,但仍(reng)存(cun)在一(yi)(yi)(yi)些(xie)(xie)問題(ti)。主(zhu)要(yao)是(shi):第一(yi)(yi)(yi):血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)可(ke)能有(you)(you)幾百種之多(duo)(duo),目(mu)前(qian)對(dui)(dui)其(qi)準(zhun)確的(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)、含量(liang)(liang)及(ji)其(qi)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)機制(zhi)(zhi)仍(reng)不清(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚,尤(you)其(qi)是(shi)對(dui)(dui)其(qi)中(zhong)一(yi)(yi)(yi)些(xie)(xie)多(duo)(duo)肽類(lei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)子、激素(su)(su)和(he)(he)(he)(he)脂類(lei)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)尚未充分(fen)認識,這(zhe)給研(yan)究(jiu)工作(zuo)(zuo)帶來許多(duo)(duo)困難(nan);第二:血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)都是(shi)批(pi)量(liang)(liang)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)(chan)(chan),各(ge)批(pi)量(liang)(liang)之間(jian)差異(yi)很大(da),而(er)(er)且血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)保存(cun)期至多(duo)(duo)一(yi)(yi)(yi)年,因(yin)(yin)此(ci)(ci),要(yao)保證每批(pi)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)相似性(xing)(xing)極為(wei)(wei)困難(nan),從而(er)(er)使(shi)實驗的(de)(de)(de)(de)(de)(de)(de)標(biao)(biao)準(zhun)化和(he)(he)(he)(he)連續性(xing)(xing)受(shou)到限制(zhi)(zhi);第三:不能排(pai)除血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)含有(you)(you)易變物(wu)質(zhi),這(zhe)被認為(wei)(wei)是(shi)“瓶中(zhong)”的(de)(de)(de)(de)(de)(de)(de)原(yuan)因(yin)(yin)之一(yi)(yi)(yi)。 雞(ji)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)瓶裝血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)凍(dong)(dong)需(xu)采(cai)用(yong)(yong)(yong)(yong)(yong)(yong)逐(zhu)步(bu)解(jie)(jie)凍(dong)(dong)法(fa):-20℃ 至 -70℃ 低溫(wen)(wen)(wen)冰箱(xiang)(xiang)(xiang)中(zhong)的(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)放入4℃冰箱(xiang)(xiang)(xiang)中(zhong)溶(rong)解(jie)(jie)1天(tian).然(ran)(ran)后(hou)移(yi)入室溫(wen)(wen)(wen),待全部(bu)溶(rong)解(jie)(jie)后(hou)再(zai)分(fen)裝.在溶(rong)解(jie)(jie)過(guo)(guo)(guo)(guo)程中(zhong)需(xu)不斷輕輕搖晃均勻(小心勿(wu)造成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)氣泡),使(shi)溫(wen)(wen)(wen)度與(yu)(yu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)均一(yi)(yi)(yi),減少沉(chen)淀(dian)的(de)(de)(de)(de)(de)(de)(de)發(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng).切勿(wu)直接(jie)將血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)從-20℃進入37℃解(jie)(jie)凍(dong)(dong),這(zhe)樣因(yin)(yin)溫(wen)(wen)(wen)度改變太大(da),容(rong)(rong)(rong)易造成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)蛋(dan)白(bai)質(zhi)凝集而(er)(er)出(chu)現沉(chen)淀(dian).雞(ji)血(xue)(xue)(xue)(xue)漿（去(qu)紅細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)、無(wu)(wu)菌(jun)(jun)(jun)過(guo)(guo)(guo)(guo)濾）雞(ji)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing) 的(de)(de)(de)(de)(de)(de)(de)其(qi)它產(chan)(chan)(chan)(chan)(chan)品(pin)(pin)(pin)(pin)特(te)點(dian):雞(ji)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)嬌貴細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)培(pei)(pei)養(yang)、細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)株保存(cun)特(te)級新(xin)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)牛血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)雜交、骨質(zhi)、原(yuan)代(dai)、傳(chuan)代(dai)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)培(pei)(pei)養(yang)及(ji)疫(yi)苗(miao)、診(zhen)斷試(shi)(shi)劑(ji)(ji)(ji)(ji)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)(chan)(chan)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)馬血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)研(yan)、診(zhen)斷試(shi)(shi)劑(ji)(ji)(ji)(ji)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)(chan)(chan)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)山(shan)羊(yang)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)研(yan)、診(zhen)斷試(shi)(shi)劑(ji)(ji)(ji)(ji)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)(chan)(chan)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)兔血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)研(yan)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)驢血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)研(yan)、診(zhen)斷試(shi)(shi)劑(ji)(ji)(ji)(ji)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)(chan)(chan)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)豬血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)研(yan)、診(zhen)斷試(shi)(shi)劑(ji)(ji)(ji)(ji)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)(chan)(chan)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)雞(ji)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)研(yan)、診(zhen)斷試(shi)(shi)劑(ji)(ji)(ji)(ji)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)(chan)(chan)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)無(wu)(wu)菌(jun)(jun)(jun)脫纖(xian)(xian)維綿羊(yang)血(xue)(xue)(xue)(xue)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)研(yan)、診(zhen)斷試(shi)(shi)劑(ji)(ji)(ji)(ji)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)(chan)(chan)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)無(wu)(wu)菌(jun)(jun)(jun)脫纖(xian)(xian)維兔血(xue)(xue)(xue)(xue)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)研(yan)、診(zhen)斷試(shi)(shi)劑(ji)(ji)(ji)(ji)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)(chan)(chan)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)羊(yang)血(xue)(xue)(xue)(xue)漿用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)研(yan)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)兔血(xue)(xue)(xue)(xue)漿用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科(ke)(ke)(ke)(ke)研(yan)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)產(chan)(chan)(chan)(chan)(chan)品(pin)(pin)(pin)(pin)應(ying)用(yong)(yong)(yong)(yong)(yong)(yong)： 用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)雜交瘤(liu)(liu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)、骨質(zhi)瘤(liu)(liu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)、原(yuan)代(dai)、傳(chuan)代(dai)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)等(deng)(deng)(deng)(deng)(deng)(deng)(deng)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)培(pei)(pei)養(yang)及(ji)疫(yi)苗(miao)、診(zhen)斷試(shi)(shi)劑(ji)(ji)(ji)(ji)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)產(chan)(chan)(chan)(chan)(chan)；雞(ji)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)IgG的(de)(de)(de)(de)(de)(de)(de)純化及(ji)其(qi)單抗(kang)(kang)制(zhi)(zhi)備與(yu)(yu)鑒定(ding)： IgG是(shi)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)主(zhu)要(yao)的(de)(de)(de)(de)(de)(de)(de)免(mian)(mian)疫(yi)球蛋(dan)白(bai)(Ig),占血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)Ig總量(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)75%~80%,它是(shi)血(xue)(xue)(xue)(xue)液中(zhong)主(zhu)要(yao)的(de)(de)(de)(de)(de)(de)(de)抗(kang)(kang)體(ti)(ti)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen),血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)IgG水平的(de)(de)(de)(de)(de)(de)(de)測(ce)定(ding),已(yi)是(shi)疫(yi)苗(miao)免(mian)(mian)疫(yi)效(xiao)(xiao)果評(ping)定(ding)和(he)(he)(he)(he)體(ti)(ti)液免(mian)(mian)疫(yi)研(yan)究(jiu)的(de)(de)(de)(de)(de)(de)(de)常用(yong)(yong)(yong)(yong)(yong)(yong)指(zhi)(zhi)標(biao)(biao)。與(yu)(yu)常規(gui)免(mian)(mian)疫(yi)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)相比(bi),單克(ke)隆抗(kang)(kang)體(ti)(ti)具(ju)(ju)有(you)(you)與(yu)(yu)抗(kang)(kang)原(yuan)結合(he)的(de)(de)(de)(de)(de)(de)(de)高特(te)異(yi)性(xing)(xing),是(shi)免(mian)(mian)疫(yi)學(xue)和(he)(he)(he)(he)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)學(xue)研(yan)究(jiu)的(de)(de)(de)(de)(de)(de)(de)重(zhong)要(yao)工具(ju)(ju),在畜牧獸醫(yi)的(de)(de)(de)(de)(de)(de)(de)疾病診(zhen)斷與(yu)(yu)治療方面已(yi)經被廣泛應(ying)用(yong)(yong)(yong)(yong)(yong)(yong)。在養(yang)禽業中(zhong),運用(yong)(yong)(yong)(yong)(yong)(yong)這(zhe)些(xie)(xie)單克(ke)隆抗(kang)(kang)體(ti)(ti)來檢(jian)測(ce)疫(yi)病特(te)異(yi)性(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)抗(kang)(kang)體(ti)(ti),對(dui)(dui)全面、系統地(di)了解(jie)(jie)禽的(de)(de)(de)(de)(de)(de)(de)機體(ti)(ti)對(dui)(dui)病原(yuan)體(ti)(ti)感(gan)染(ran)的(de)(de)(de)(de)(de)(de)(de)免(mian)(mian)疫(yi)應(ying)答反應(ying)并采(cai)取相應(ying)的(de)(de)(de)(de)(de)(de)(de)防制(zhi)(zhi)措施有(you)(you)重(zhong)要(yao)意(yi)義。雞(ji)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)背景 雞(ji)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)培(pei)(pei)養(yang)中(zhong)用(yong)(yong)(yong)(yong)(yong)(yong)量(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)天(tian)然(ran)(ran)培(pei)(pei)養(yang)基，含有(you)(you)豐富的(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)必(bi)須(xu)的(de)(de)(de)(de)(de)(de)(de)營養(yang)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)，常用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)動(dong)物(wu)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)體(ti)(ti)外培(pei)(pei)養(yang)，具(ju)(ju)有(you)(you)極為(wei)(wei)重(zhong)要(yao)的(de)(de)(de)(de)(de)(de)(de)功能。血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)必(bi)須(xu)貯存(cun)于(yu)(yu)(yu)(yu)(yu)–20～-70 oC，若(ruo)存(cun)放于(yu)(yu)(yu)(yu)(yu)4度，不能超過(guo)(guo)(guo)(guo)一(yi)(yi)(yi)個月。如果一(yi)(yi)(yi)次無(wu)(wu)法(fa)用(yong)(yong)(yong)(yong)(yong)(yong)完(wan)一(yi)(yi)(yi)瓶，可(ke)將40～45 ml分(fen)裝于(yu)(yu)(yu)(yu)(yu)無(wu)(wu)菌(jun)(jun)(jun)50 ml離(li)心管中(zhong)，由于(yu)(yu)(yu)(yu)(yu)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)結凍(dong)(dong)時體(ti)(ti)積會增加約10%，必(bi)須(xu)預留10%膨脹(zhang)體(ti)(ti)積的(de)(de)(de)(de)(de)(de)(de)空間(jian)，否則(ze)易發(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)污(wu)染(ran)或(huo)容(rong)(rong)(rong)器凍(dong)(dong)裂。 瓶裝(500ml)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)凍(dong)(dong)步(bu)驟(逐(zhu)步(bu)解(jie)(jie)凍(dong)(dong)法(fa)):從-20 oC或(huo)–70 oC取出(chu)放至4 oC冰箱(xiang)(xiang)(xiang)溶(rong)解(jie)(jie)一(yi)(yi)(yi)天(tian)，再(zai)置(zhi)(zhi)于(yu)(yu)(yu)(yu)(yu)室溫(wen)(wen)(wen)下全溶(rong)后(hou)分(fen)裝，一(yi)(yi)(yi)般50 ml無(wu)(wu)菌(jun)(jun)(jun)離(li)心管可(ke)分(fen)裝40～45 ml。在溶(rong)解(jie)(jie)過(guo)(guo)(guo)(guo)程中(zhong)須(xu)規(gui)則(ze)搖晃均勻(小心勿(wu)造成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)氣泡)，使(shi)溫(wen)(wen)(wen)度與(yu)(yu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)均一(yi)(yi)(yi)，減少沈(shen)淀(dian)的(de)(de)(de)(de)(de)(de)(de)發(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)。切勿(wu)直接(jie)由–20 oC直接(jie)至37 oC解(jie)(jie)凍(dong)(dong)，因(yin)(yin)溫(wen)(wen)(wen)度改變太大(da)，容(rong)(rong)(rong)易造成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)蛋(dan)白(bai)質(zhi)凝結而(er)(er)發(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)沈(shen)淀(dian)。勿(wu)將血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)置(zhi)(zhi)于(yu)(yu)(yu)(yu)(yu)37 oC太久，若(ruo)在37 oC放置(zhi)(zhi)太久，血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)會變得混濁(zhuo)，同時血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)許多(duo)(duo)不穩(wen)定(ding)的(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)份(fen)也(ye)會因(yin)(yin)此(ci)(ci)受(shou)到破(po)壞(huai)，從而(er)(er)影響(xiang)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)品(pin)(pin)(pin)(pin)質(zhi)。
馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)（去紅細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、無(wu)(wu)(wu)菌(jun)過(guo)(guo)(guo)濾(lv)）產(chan)品(pin)介(jie)紹(shao) 采用(yong)(yong)(yong)(yong)(yong)(yong)(yong)健康動物血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液為原(yuan)(yuan)料(liao)，經無(wu)(wu)(wu)菌(jun)采集，分(fen)離(li)，微孔過(guo)(guo)(guo)濾(lv)后而成(cheng)(cheng)(cheng)，性(xing)(xing)(xing)(xing)(xing)狀(zhuang)為橙清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液體(ti)(ti)(ti)(ti)，無(wu)(wu)(wu)噬菌(jun)體(ti)(ti)(ti)(ti)，無(wu)(wu)(wu)溶(rong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)，無(wu)(wu)(wu)異物，無(wu)(wu)(wu)細(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)，真菌(jun)支(zhi)原(yuan)(yuan)體(ti)(ti)(ti)(ti)毒形體(ti)(ti)(ti)(ti)衣原(yuan)(yuan)體(ti)(ti)(ti)(ti)等(deng)，適(shi)(shi)應于(yu)(yu)試(shi)驗室或生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)科研(yan)相關試(shi)驗，滿足(zu)不(bu)同科研(yan)實驗的(de)(de)(de)(de)(de)(de)(de)多種(zhong)需求注(zhu)意事(shi)項1、注(zhu)意避免反(fan)(fan)復凍融。反(fan)(fan)復凍融可(ke)(ke)(ke)(ke)能產(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)沉淀，可(ke)(ke)(ke)(ke)以(yi)通(tong)(tong)過(guo)(guo)(guo)無(wu)(wu)(wu)菌(jun)條件下(xia)，3500 rp m離(li)心(xin)10 min除去沉淀。2、注(zhu)意無(wu)(wu)(wu)菌(jun)操作(zuo)。標簽：馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)說明(ming)書 ；馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)規格(ge)產(chan)品(pin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)途1、可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)或誘導(dao)樹(shu)突(tu)(tu)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)，且細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)，增殖(zhi)(zhi)狀(zhuang)況良好 有(you)(you)(you)(you)研(yan)究人(ren)員分(fen)別(bie)(bie)(bie)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)胎(tai)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)和(he)(he)馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)馬(ma)(ma)單(dan)核(he)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)來產(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)權突(tu)(tu)狀(zhuang)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)（eqMoDC），結果發(fa)(fa)(fa)(fa)現(xian)(xian)，馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)生(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)eqMoDC與FBS生(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)eqMoDC沒有(you)(you)(you)(you)區別(bie)(bie)(bie)。然而，與馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)補(bu)充(chong)培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)基(ji)(ji)一(yi)(yi)起(qi)孵(fu)育(yu)(yu)的(de)(de)(de)(de)(de)(de)(de)e qMo DC在(zai)(zai)(zai)(zai)從(cong)單(dan)核(he)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)分(fen)化(hua)過(guo)(guo)(guo)程中(zhong)(zhong)表現(xian)(xian)出(chu)更(geng)(geng)具(ju)特征性(xing)(xing)(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)樹(shu)突(tu)(tu)狀(zhuang)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)形態。在(zai)(zai)(zai)(zai)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)eqMoDC中(zhong)(zhong)也觀(guan)察到(dao)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)活(huo)力(li)的(de)(de)(de)(de)(de)(de)(de)顯(xian)著(zhu)增加(jia)(jia)(jia)。此外，發(fa)(fa)(fa)(fa)現(xian)(xian)在(zai)(zai)(zai)(zai)馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)存在(zai)(zai)(zai)(zai)下(xia)產(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)的(de)(de)(de)(de)(de)(de)(de)eqMoDC在(zai)(zai)(zai)(zai)功(gong)能性(xing)(xing)(xing)(xing)(xing)T淋巴細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)啟動能力(li)方(fang)面優越，并且引發(fa)(fa)(fa)(fa)的(de)(de)(de)(de)(de)(de)(de)非(fei)特異性(xing)(xing)(xing)(xing)(xing)增殖(zhi)(zhi)顯(xian)著(zhu)減少。2、可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)原(yuan)(yuan)代培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)神經細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao) 有(you)(you)(you)(you)人(ren)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)含(han)10%馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)DMEM，分(fen)離(li)培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)BALB/c小(xiao)(xiao)鼠(shu)(shu)原(yuan)(yuan)代腦神經細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)。發(fa)(fa)(fa)(fa)現(xian)(xian)和(he)(he)添(tian)加(jia)(jia)(jia)2%神經細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)劑(ji)B27的(de)(de)(de)(de)(de)(de)(de)Neurobasal培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)基(ji)(ji)相比，含(han)10%馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)DMEM培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)基(ji)(ji)更(geng)(geng)節省成(cheng)(cheng)(cheng)本(ben)(ben)，方(fang)便廉價。3、特定脂(zhi)肪酸(suan)產(chan)物濃(nong)度(du)(du)的(de)(de)(de)(de)(de)(de)(de)馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),對造血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)有(you)(you)(you)(you)支(zhi)持作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong) 研(yan)究表明(ming)，磷脂(zhi)酶A2特定脂(zhi)肪酸(suan)產(chan)物的(de)(de)(de)(de)(de)(de)(de)濃(nong)度(du)(du)馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，支(zhi)持多能小(xiao)(xiao)鼠(shu)(shu)造血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)祖細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)FDCP-Mix細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)自(zi)我更(geng)(geng)新的(de)(de)(de)(de)(de)(de)(de)能力(li)。4、可(ke)(ke)(ke)(ke)增強(qiang)豬(zhu)(zhu)(zhu)單(dan)性(xing)(xing)(xing)(xing)(xing)生(sheng)(sheng)(sheng)(sheng)(sheng)殖(zhi)(zhi)胚(pei)(pei)胎(tai)植(zhi)(zhi)入(ru)前(qian)(qian)，胚(pei)(pei)層的(de)(de)(de)(de)(de)(de)(de)發(fa)(fa)(fa)(fa)育(yu)(yu)。 有(you)(you)(you)(you)研(yan)究通(tong)(tong)過(guo)(guo)(guo)對比不(bu)同血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)和(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)樣(yang)(yang)物質(zhi)(zhi)(zhi)(zhi)，對豬(zhu)(zhu)(zhu)單(dan)性(xing)(xing)(xing)(xing)(xing)生(sheng)(sheng)(sheng)(sheng)(sheng)殖(zhi)(zhi)胚(pei)(pei)胎(tai)植(zhi)(zhi)入(ru)前(qian)(qian)發(fa)(fa)(fa)(fa)育(yu)(yu)的(de)(de)(de)(de)(de)(de)(de)影響，尋找適(shi)(shi)合其(qi)發(fa)(fa)(fa)(fa)育(yu)(yu)的(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)基(ji)(ji)。對單(dan)性(xing)(xing)(xing)(xing)(xing)生(sheng)(sheng)(sheng)(sheng)(sheng)殖(zhi)(zhi)胚(pei)(pei)胎(tai)進行(xing)豬(zhu)(zhu)(zhu)濾(lv)泡液（PFF)、胎(tai)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(（FBS)、馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（HS）或豬(zhu)(zhu)(zhu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)白(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)（PSA)處理，培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)2天后。通(tong)(tong)過(guo)(guo)(guo)囊胚(pei)(pei)形成(cheng)(cheng)(cheng)和(he)(he)孵(fu)化(hua)，發(fa)(fa)(fa)(fa)現(xian)(xian)馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)增強(qiang)胚(pei)(pei)層發(fa)(fa)(fa)(fa)育(yu)(yu)的(de)(de)(de)(de)(de)(de)(de)＊有(you)(you)(you)(you)效蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)(zhi)來源(yuan)。接下(xia)來，使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)三種(zhong)不(bu)同濃(nong)度(du)(du)的(de)(de)(de)(de)(de)(de)(de)HS (10%，20%和(he)(he)30%）來確定改善豬(zhu)(zhu)(zhu)單(dan)性(xing)(xing)(xing)(xing)(xing)生(sheng)(sheng)(sheng)(sheng)(sheng)殖(zhi)(zhi)胚(pei)(pei)胎(tai)發(fa)(fa)(fa)(fa)育(yu)(yu)所需的(de)(de)(de)(de)(de)(de)(de)適(shi)(shi)宜HS濃(nong)度(du)(du)。所有(you)(you)(you)(you)HS濃(nong)度(du)(du)均(jun)增加(jia)(jia)(jia)了(le)囊胚(pei)(pei)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)數量(liang)，降低(di)了(le)囊胚(pei)(pei)凋(diao)亡(wang)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)發(fa)(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)率，其(qi)中(zhong)(zhong)20%效果更(geng)(geng)顯(xian)著(zhu)。5、馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)添(tian)加(jia)(jia)(jia)到(dao)微生(sheng)(sheng)(sheng)(sheng)(sheng)物培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)基(ji)(ji)中(zhong)(zhong)，可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)微生(sheng)(sheng)(sheng)(sheng)(sheng)物，如∶(1）馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)添(tian)加(jia)(jia)(jia)到(dao)培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)基(ji)(ji)中(zhong)(zhong)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)支(zhi)原(yuan)(yuan)體(ti)(ti)(ti)(ti)的(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)。在(zai)(zai)(zai)(zai)《獸藥典》中(zhong)(zhong)，含(han)馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)支(zhi)原(yuan)(yuan)體(ti)(ti)(ti)(ti)培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)基(ji)(ji)被用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)獸用(yong)(yong)(yong)(yong)(yong)(yong)(yong)疫苗(miao)的(de)(de)(de)(de)(de)(de)(de)支(zhi)原(yuan)(yuan)體(ti)(ti)(ti)(ti)檢查。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)KM2培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)基(ji)(ji)(含(han)馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing))還可(ke)(ke)(ke)(ke)培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)豬(zhu)(zhu)(zhu)肺炎(yan)支(zhi)原(yuan)(yuan)體(ti)(ti)(ti)(ti)。(2）馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)加(jia)(jia)(jia)入(ru)到(dao)固體(ti)(ti)(ti)(ti)和(he)(he)液體(ti)(ti)(ti)(ti)培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)基(ji)(ji)中(zhong)(zhong)，可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)幽門(men)螺桿菌(jun)。并且，和(he)(he)羊(yang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)和(he)(he)牛血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)比較(jiao)，添(tian)加(jia)(jia)(jia)馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)基(ji)(ji)更(geng)(geng)易于(yu)(yu)分(fen)裝(zhuang)和(he)(he)保存。馬(ma)(ma)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)（去紅細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、無(wu)(wu)(wu)菌(jun)過(guo)(guo)(guo)濾(lv)）檢驗報告(gao)：檢測(ce)項目檢測(ce)結果外觀(guan)淡(dan)黃色(se)液體(ti)(ti)(ti)(ti)PH7.26無(wu)(wu)(wu)菌(jun)檢驗陰(yin)性(xing)(xing)(xing)(xing)(xing)總蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)68.6g/L白(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)35.1g/L支(zhi)原(yuan)(yuan)體(ti)(ti)(ti)(ti)陰(yin)性(xing)(xing)(xing)(xing)(xing)內(nei)毒素(su)(su)(su)(su)8UE/mL血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)紅蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)0.04g/L血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)主要(yao)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)●提供(gong)基(ji)(ji)本(ben)(ben)營養(yang)(yang)物質(zhi)(zhi)(zhi)(zhi)：氨基(ji)(ji)酸(suan)、維生(sheng)(sheng)(sheng)(sheng)(sheng)素(su)(su)(su)(su)、無(wu)(wu)(wu)機(ji)(ji)物、脂(zhi)類物質(zhi)(zhi)(zhi)(zhi)、核(he)酸(suan)衍生(sheng)(sheng)(sheng)(sheng)(sheng)物等(deng)，是(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)必(bi)須(xu)的(de)(de)(de)(de)(de)(de)(de)物質(zhi)(zhi)(zhi)(zhi)。●提供(gong)激素(su)(su)(su)(su)和(he)(he)各種(zhong)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)：胰(yi)島素(su)(su)(su)(su)、腎上腺皮質(zhi)(zhi)(zhi)(zhi)激素(su)(su)(su)(su)（氫化(hua)可(ke)(ke)(ke)(ke)的(de)(de)(de)(de)(de)(de)(de)松、地塞米松）、類固醇激素(su)(su)(su)(su)（雌二(er)醇、睪酮(tong)、孕酮(tong)）等(deng)。生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)如成(cheng)(cheng)(cheng)纖維細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)、表皮生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)板生(sheng)(sheng)(sheng)(sheng)(sheng)長(chang)因(yin)(yin)(yin)子(zi)等(deng)。●提供(gong)結合蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)：結合蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)是(shi)(shi)攜帶(dai)重(zhong)要(yao)的(de)(de)(de)(de)(de)(de)(de)低(di)分(fen)子(zi)量(liang)物質(zhi)(zhi)(zhi)(zhi)，如白(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)攜帶(dai)維生(sheng)(sheng)(sheng)(sheng)(sheng)素(su)(su)(su)(su)、脂(zhi)肪、以(yi)及(ji)激素(su)(su)(su)(su)等(deng)，轉(zhuan)鐵蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)攜帶(dai)鐵。結合蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)在(zai)(zai)(zai)(zai)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)代謝過(guo)(guo)(guo)程中(zhong)(zhong)起(qi)重(zhong)要(yao)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。●提供(gong)促接觸和(he)(he)伸展因(yin)(yin)(yin)子(zi)使細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)貼(tie)壁(bi)免受機(ji)(ji)械損(sun)傷。●對培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)中(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)起(qi)到(dao)某些(xie)(xie)保護作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)：有(you)(you)(you)(you)一(yi)(yi)些(xie)(xie)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)，如內(nei)皮細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)、骨(gu)髓樣(yang)(yang)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)可(ke)(ke)(ke)(ke)以(yi)釋(shi)放蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)酶，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)含(han)有(you)(you)(you)(you)抗(kang)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)酶成(cheng)(cheng)(cheng)分(fen)，起(qi)到(dao)中(zhong)(zhong)和(he)(he)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。這(zhe)種(zhong)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)是(shi)(shi)偶然發(fa)(fa)(fa)(fa)現(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)，則(ze)有(you)(you)(you)(you)目的(de)(de)(de)(de)(de)(de)(de)的(de)(de)(de)(de)(de)(de)(de)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)來終止胰(yi)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)酶的(de)(de)(de)(de)(de)(de)(de)消化(hua)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。因(yin)(yin)(yin)為胰(yi)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)酶已經被廣泛用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)貼(tie)壁(bi)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)消化(hua)傳代。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)形成(cheng)(cheng)(cheng)了(le)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)粘度(du)(du)，可(ke)(ke)(ke)(ke)以(yi)保護細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)免受機(ji)(ji)械損(sun)傷，特別(bie)(bie)(bie)是(shi)(shi)在(zai)(zai)(zai)(zai)懸浮(fu)培(pei)(pei)(pei)(pei)(pei)養(yang)(yang)攪(jiao)拌時，粘度(du)(du)起(qi)到(dao)重(zhong)要(yao)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)還含(han)有(you)(you)(you)(you)一(yi)(yi)些(xie)(xie)微量(liang)元(yuan)素(su)(su)(su)(su)和(he)(he)離(li)子(zi)，他們在(zai)(zai)(zai)(zai)代謝解毒中(zhong)(zhong)起(qi)重(zhong)要(yao)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如SeO3，硒等(deng)。鑒別(bie)(bie)(bie)方(fang)法 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液凝(ning)固析出(chu)的(de)(de)(de)(de)(de)(de)(de)淡(dan)黃色(se)透(tou)(tou)明(ming)液體(ti)(ti)(ti)(ti) 。如將血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液自(zi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管(guan)(guan)內(nei)抽出(chu)，放入(ru)試(shi)管(guan)(guan)中(zhong)(zhong)，不(bu)加(jia)(jia)(jia)抗(kang)凝(ning)劑(ji)，則(ze)凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)(fan)應被激活(huo)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液迅速凝(ning)固，形成(cheng)(cheng)(cheng)膠凍。凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)塊收縮，其(qi)周(zhou)圍所析出(chu)之淡(dan)黃色(se)透(tou)(tou)明(ming)液體(ti)(ti)(ti)(ti)即(ji)為血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，也可(ke)(ke)(ke)(ke)于(yu)(yu)凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后經離(li)心(xin)取得(de)。在(zai)(zai)(zai)(zai)凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)過(guo)(guo)(guo)程中(zhong)(zhong)，纖維蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)原(yuan)(yuan)轉(zhuan)變(bian)(bian)成(cheng)(cheng)(cheng)纖維蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)塊，所以(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)無(wu)(wu)(wu)纖維蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)原(yuan)(yuan)，這(zhe)一(yi)(yi)點(dian)是(shi)(shi)與血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)＊大的(de)(de)(de)(de)(de)(de)(de)區別(bie)(bie)(bie)。而在(zai)(zai)(zai)(zai)凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)(fan)應中(zhong)(zhong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)(xiao)板釋(shi)放出(chu)許多物質(zhi)(zhi)(zhi)(zhi)，各凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)因(yin)(yin)(yin)子(zi)也都發(fa)(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)了(le)變(bian)(bian)化(hua)。這(zhe)些(xie)(xie)成(cheng)(cheng)(cheng)分(fen)都留在(zai)(zai)(zai)(zai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)并繼續(xu)發(fa)(fa)(fa)(fa)生(sheng)(sheng)(sheng)(sheng)(sheng)變(bian)(bian)化(hua)，如凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)酶原(yuan)(yuan)變(bian)(bian)成(cheng)(cheng)(cheng)凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)酶，并隨血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)存放時間(jian)逐漸減少以(yi)至(zhi)消失。這(zhe)些(xie)(xie)也都是(shi)(shi)與血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)區別(bie)(bie)(bie)之處。但大量(liang)未參加(jia)(jia)(jia)凝(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)(fan)應的(de)(de)(de)(de)(de)(de)(de)物質(zhi)(zhi)(zhi)(zhi)則(ze)與血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)基(ji)(ji)本(ben)(ben)相同。為避免抗(kang)凝(ning)劑(ji)的(de)(de)(de)(de)(de)(de)(de)干擾，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液中(zhong)(zhong)許多化(hua)學成(cheng)(cheng)(cheng)分(fen)的(de)(de)(de)(de)(de)(de)(de)分(fen)析，都以(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)為樣(yang)(yang)品(pin)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液的(de)(de)(de)(de)(de)(de)(de)液體(ti)(ti)(ti)(ti)成(cheng)(cheng)(cheng)分(fen)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)懸濁(zhuo)于(yu)(yu)其(qi)中(zhong)(zhong)。人(ren)體(ti)(ti)(ti)(ti)含(han)有(you)(you)(you)(you)2750-3300毫(hao)升血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)，約(yue)占血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液總體(ti)(ti)(ti)(ti)積的(de)(de)(de)(de)(de)(de)(de)55%。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)的(de)(de)(de)(de)(de)(de)(de)絕大部分(fen)是(shi)(shi)水（體(ti)(ti)(ti)(ti)積的(de)(de)(de)(de)(de)(de)(de)90%），其(qi)中(zhong)(zhong)溶(rong)解的(de)(de)(de)(de)(de)(de)(de)物質(zhi)(zhi)(zhi)(zhi)主要(yao)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)，還包(bao)括葡萄糖、無(wu)(wu)(wu)機(ji)(ji)鹽離(li)子(zi)、激素(su)(su)(su)(su)以(yi)及(ji)二(er)氧(yang)化(hua)碳。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)的(de)(de)(de)(de)(de)(de)(de)主要(yao)功(gong)能是(shi)(shi)運載(zai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)，同時也是(shi)(shi)運輸分(fen)泌產(chan)物的(de)(de)(de)(de)(de)(de)(de)主要(yao)媒介(jie)。
兔(tu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)（去紅(hong)細(xi)(xi)(xi)胞(bao)、無(wu)(wu)菌(jun)過濾(lv)）產(chan)品(pin)(pin)描述血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)采集(ji)自(zi)(zi)正(zheng)常(chang)(chang)(chang)健康(kang)的(de)(de)(de)(de)(de)(de)(de)(de)供(gong)體(ti)(ti)，經(jing)脂(zhi)質(zhi)萃(cui)取(qu)和透(tou)析(xi)(xi)后(hou)儲存于(yu)(yu)(yu)(yu)緩(huan)沖(chong)(chong)(chong)液(ye)(ye)(ye)(ye)(ye)(ye)（0.01 M磷酸鈉，0.25 M氯化(hua)(hua)鈉, pH 7.6）中(zhong)(zhong)(zhong)。常(chang)(chang)(chang)用(yong)于(yu)(yu)(yu)(yu)各(ge)種免疫實驗(yan)，起(qi)到封閉或(huo)對照(zhao)(zhao)的(de)(de)(de)(de)(de)(de)(de)(de)作(zuo)(zuo)用(yong)。本(ben)品(pin)(pin)用(yong)于(yu)(yu)(yu)(yu)封閉的(de)(de)(de)(de)(de)(de)(de)(de)常(chang)(chang)(chang)見使(shi)用(yong)濃(nong)度(du)(du)(du)為(wei)(wei)5%（v/v）。產(chan)品(pin)(pin)使(shi)用(yong)1）血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)以(yi)(yi)(yi)(yi)凍(dong)(dong)(dong)(dong)干粉形式提(ti)供(gong)的(de)(de)(de)(de)(de)(de)(de)(de)，本(ben)品(pin)(pin)需(xu)(xu)要先溶(rong)(rong)(rong)(rong)解(jie)(jie)配制(zhi)成(cheng)(cheng)100%原(yuan)(yuan)(yuan)液(ye)(ye)(ye)(ye)(ye)(ye)后(hou)再稀(xi)釋(shi)使(shi)用(yong)。溶(rong)(rong)(rong)(rong)解(jie)(jie)方(fang)(fang)法(fa)：用(yong)10ml 蒸(zheng)餾水(shui)（dH2O）充(chong)分(fen)(fen)(fen)溶(rong)(rong)(rong)(rong)解(jie)(jie)凍(dong)(dong)(dong)(dong)干粉（如(ru)果不澄清(qing)(qing)(qing)(qing)(qing)，可(ke)(ke)對其(qi)離(li)(li)心(xin)處(chu)(chu)(chu)理），此時(shi)(shi)得到的(de)(de)(de)(de)(de)(de)(de)(de)即是(shi)(shi)(shi)100%原(yuan)(yuan)(yuan)液(ye)(ye)(ye)(ye)(ye)(ye)。然后(hou)按(an)照(zhao)(zhao)1:20的(de)(de)(de)(de)(de)(de)(de)(de)比例用(yong)緩(huan)沖(chong)(chong)(chong)液(ye)(ye)(ye)(ye)(ye)(ye)，如(ru)PBS或(huo)PBST，稀(xi)釋(shi)到需(xu)(xu)要的(de)(de)(de)(de)(de)(de)(de)(de)5%工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)。也(ye)(ye)可(ke)(ke)以(yi)(yi)(yi)(yi)根據(ju)自(zi)(zi)身(shen)實驗(yan)條(tiao)件(jian)，稀(xi)釋(shi)到合(he)適的(de)(de)(de)(de)(de)(de)(de)(de)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)濃(nong)度(du)(du)(du)。2）血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)以(yi)(yi)(yi)(yi)100%原(yuan)(yuan)(yuan)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)形式提(ti)供(gong)，直(zhi)接用(yong)合(he)適的(de)(de)(de)(de)(de)(de)(de)(de)緩(huan)沖(chong)(chong)(chong)液(ye)(ye)(ye)(ye)(ye)(ye)稀(xi)釋(shi)到需(xu)(xu)要的(de)(de)(de)(de)(de)(de)(de)(de)工(gong)(gong)作(zuo)(zuo)液(ye)(ye)(ye)(ye)(ye)(ye)。產(chan)品(pin)(pin)性質(zhi)蛋(dan)白(bai)濃(nong)度(du)(du)(du)（Protein concentration）60 mg/mL緩(huan)沖(chong)(chong)(chong)液(ye)(ye)(ye)(ye)(ye)(ye)（Buffer）0.01 M磷酸鈉，0.25 M氯化(hua)(hua)鈉, pH 7.6防(fang)腐劑(ji)（Preservative）0.05%疊氮(dan)化(hua)(hua)鈉運(yun)輸(shu)與(yu)(yu)(yu)保(bao)(bao)(bao)存方(fang)(fang)法(fa)冰(bing)袋運(yun)輸(shu)。凍(dong)(dong)(dong)(dong)干粉保(bao)(bao)(bao)存于(yu)(yu)(yu)(yu)2～8℃。100%原(yuan)(yuan)(yuan)液(ye)(ye)(ye)(ye)(ye)(ye)保(bao)(bao)(bao)存于(yu)(yu)(yu)(yu)2～8℃，6個(ge)星期穩(wen)定。建議原(yuan)(yuan)(yuan)液(ye)(ye)(ye)(ye)(ye)(ye)分(fen)(fen)(fen)裝(zhuang)后(hou)置于(yu)(yu)(yu)(yu)≤-20℃，以(yi)(yi)(yi)(yi)延(yan)長保(bao)(bao)(bao)存期。重溶(rong)(rong)(rong)(rong)后(hou)100%原(yuan)(yuan)(yuan)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)有效期1年。注(zhu)意事項1）本(ben)品(pin)(pin)含疊氮(dan)化(hua)(hua)鈉，對人體(ti)(ti)有害，請(qing)注(zhu)意適當防(fang)護。2）為(wei)(wei)了您的(de)(de)(de)(de)(de)(de)(de)(de)安(an)全(quan)和健康(kang)，請(qing)穿實驗(yan)服并(bing)戴一次性手套操(cao)作(zuo)(zuo)。3）注(zhu)意無(wu)(wu)菌(jun)操(cao)作(zuo)(zuo)4）注(zhu)意避免反(fan)復(fu)凍(dong)(dong)(dong)(dong)融。反(fan)復(fu)凍(dong)(dong)(dong)(dong)融可(ke)(ke)能產(chan)生(sheng)沉(chen)淀，可(ke)(ke)以(yi)(yi)(yi)(yi)通(tong)過無(wu)(wu)菌(jun)條(tiao)件(jian)下(xia)，3500rpm離(li)(li)心(xin)10min除去沉(chen)淀兔(tu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)（去紅(hong)細(xi)(xi)(xi)胞(bao)、無(wu)(wu)菌(jun)過濾(lv)）常(chang)(chang)(chang)見問題 保(bao)(bao)(bao)存方(fang)(fang)法(fa)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)應(ying)(ying)(ying)保(bao)(bao)(bao)存在(zai)(zai)-5℃至(zhi)(zhi)(zhi)-20℃。然而，若存放于(yu)(yu)(yu)(yu)4℃時(shi)(shi)，請(qing)勿超(chao)過一個(ge)月。若您一次無(wu)(wu)法(fa)用(yong)完一瓶，建議將(jiang)無(wu)(wu)菌(jun)分(fen)(fen)(fen)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)至(zhi)(zhi)(zhi)恰當的(de)(de)(de)(de)(de)(de)(de)(de)滅(mie)菌(jun)容器內(nei)，再放回冷凍(dong)(dong)(dong)(dong)。解(jie)(jie)凍(dong)(dong)(dong)(dong)方(fang)(fang)法(fa)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)從冷凍(dong)(dong)(dong)(dong)箱(xiang)取(qu)出(chu)(chu)(chu)后(hou)，先置于(yu)(yu)(yu)(yu)2-8℃冰(bing)箱(xiang)使(shi)之(zhi)(zhi)(zhi)溶(rong)(rong)(rong)(rong)解(jie)(jie)，然后(hou)在(zai)(zai)室溫下(xia)使(shi)之(zhi)(zhi)(zhi)全(quan)溶(rong)(rong)(rong)(rong)。但必須注(zhu)意的(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)，溶(rong)(rong)(rong)(rong)解(jie)(jie)過程中(zhong)(zhong)(zhong)必須規則地搖晃均勻(yun)。避免產(chan)生(sheng)沉(chen)淀物(wu)1、解(jie)(jie)凍(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)時(shi)(shi)，請(qing)按(an)照(zhao)(zhao)所(suo)建議的(de)(de)(de)(de)(de)(de)(de)(de)逐步解(jie)(jie)凍(dong)(dong)(dong)(dong)法(fa)（-2℃至(zhi)(zhi)(zhi)4℃至(zhi)(zhi)(zhi)室溫），若血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)解(jie)(jie)凍(dong)(dong)(dong)(dong)時(shi)(shi)改變(bian)(bian)的(de)(de)(de)(de)(de)(de)(de)(de)溫度(du)(du)(du)太(tai)大（如(ru)-20℃至(zhi)(zhi)(zhi)37℃），實驗(yan)顯(xian)示非常(chang)(chang)(chang)容易(yi)產(chan)生(sheng)沉(chen)淀物(wu)。2、解(jie)(jie)凍(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)時(shi)(shi)，請(qing)隨(sui)時(shi)(shi)將(jiang)之(zhi)(zhi)(zhi)搖晃均勻(yun)，使(shi)溫度(du)(du)(du)及成(cheng)(cheng)分(fen)(fen)(fen)均一，減少(shao)沉(chen)淀的(de)(de)(de)(de)(de)(de)(de)(de)發生(sheng)。3、請(qing)勿將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)置于(yu)(yu)(yu)(yu)37℃太(tai)久(jiu)。若在(zai)(zai)37℃放置太(tai)久(jiu)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)會變(bian)(bian)得混濁，同時(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)許多較不穩(wen)定的(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)分(fen)(fen)(fen)也(ye)(ye)會因(yin)此受到損害，而影響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)量(liang)。4、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)熱滅(mie)活(huo)(huo)非常(chang)(chang)(chang)容易(yi)造(zao)成(cheng)(cheng)沉(chen)淀物(wu)的(de)(de)(de)(de)(de)(de)(de)(de)增(zeng)多，若非必要，可(ke)(ke)以(yi)(yi)(yi)(yi)無(wu)(wu)須做此步驟。 [3]5、若必須做血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)熱滅(mie)活(huo)(huo)，請(qing)遵(zun)守56℃，30分(fen)(fen)(fen)鐘的(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)(yuan)(yuan)則，并(bing)且(qie)隨(sui)時(shi)(shi)搖晃均勻(yun)。溫度(du)(du)(du)過高，時(shi)(shi)間過久(jiu)或(huo)搖晃不均勻(yun)，都(dou)(dou)會造(zao)成(cheng)(cheng)沉(chen)淀物(wu)的(de)(de)(de)(de)(de)(de)(de)(de)增(zeng)多。若血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)解(jie)(jie)凍(dong)(dong)(dong)(dong)后(hou)發現有絮狀沉(chen)淀物(wu)出(chu)(chu)(chu)現，可(ke)(ke)以(yi)(yi)(yi)(yi)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)分(fen)(fen)(fen)裝(zhuang)至(zhi)(zhi)(zhi)無(wu)(wu)菌(jun)離(li)(li)心(xin)管(guan)內(nei)，以(yi)(yi)(yi)(yi)400g稍微離(li)(li)心(xin)，上(shang)清(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)即可(ke)(ke)接著加入培養基(ji)內(nei)一起(qi)過濾(lv)。但不建議以(yi)(yi)(yi)(yi)過濾(lv)的(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)法(fa)去除這(zhe)些絮狀沉(chen)淀物(wu)，因(yin)為(wei)(wei)它可(ke)(ke)能會阻塞過濾(lv)膜。熱滅(mie)活(huo)(huo)一般(ban)是(shi)(shi)(shi)以(yi)(yi)(yi)(yi)56℃，30分(fen)(fen)(fen)鐘來(lai)處(chu)(chu)(chu)理已(yi)解(jie)(jie)凍(dong)(dong)(dong)(dong)的(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)，因(yin)為(wei)(wei)此加熱步驟，可(ke)(ke)以(yi)(yi)(yi)(yi)使(shi)補體(ti)(ti)去活(huo)(huo)化(hua)(hua)，而補體(ti)(ti)所(suo)參與(yu)(yu)(yu)的(de)(de)(de)(de)(de)(de)(de)(de)反(fan)應(ying)(ying)(ying)有：溶(rong)(rong)(rong)(rong)細(xi)(xi)(xi)胞(bao)活(huo)(huo)性，平滑(hua)肌的(de)(de)(de)(de)(de)(de)(de)(de)收縮(suo)，肥大細(xi)(xi)(xi)胞(bao)和血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)板(ban)組胺的(de)(de)(de)(de)(de)(de)(de)(de)釋(shi)放，增(zeng)強吞噬(shi)作(zuo)(zuo)用(yong)，淋巴(ba)細(xi)(xi)(xi)胞(bao)、巨噬(shi)細(xi)(xi)(xi)胞(bao)的(de)(de)(de)(de)(de)(de)(de)(de)趨化(hua)(hua)和激(ji)活(huo)(huo)。鑒(jian)別方(fang)(fang)法(fa) 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)凝(ning)(ning)(ning)固析(xi)(xi)出(chu)(chu)(chu)的(de)(de)(de)(de)(de)(de)(de)(de)淡(dan)(dan)黃(huang)色(se)透(tou)明液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti) 。如(ru)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)自(zi)(zi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)管(guan)內(nei)抽出(chu)(chu)(chu)，放入試管(guan)中(zhong)(zhong)(zhong)，不加抗(kang)凝(ning)(ning)(ning)劑(ji)，則凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)應(ying)(ying)(ying)被激(ji)活(huo)(huo)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)迅(xun)速(su)凝(ning)(ning)(ning)固，形成(cheng)(cheng)膠(jiao)凍(dong)(dong)(dong)(dong)。凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)塊(kuai)收縮(suo)，其(qi)周圍所(suo)析(xi)(xi)出(chu)(chu)(chu)之(zhi)(zhi)(zhi)淡(dan)(dan)黃(huang)色(se)透(tou)明液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)即為(wei)(wei)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)，也(ye)(ye)可(ke)(ke)于(yu)(yu)(yu)(yu)凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)后(hou)經(jing)離(li)(li)心(xin)取(qu)得。在(zai)(zai)凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)過程中(zhong)(zhong)(zhong)，纖(xian)維蛋(dan)白(bai)原(yuan)(yuan)(yuan)轉變(bian)(bian)成(cheng)(cheng)纖(xian)維蛋(dan)白(bai)塊(kuai)，所(suo)以(yi)(yi)(yi)(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)無(wu)(wu)纖(xian)維蛋(dan)白(bai)原(yuan)(yuan)(yuan)，這(zhe)一點是(shi)(shi)(shi)與(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)＊大的(de)(de)(de)(de)(de)(de)(de)(de)區(qu)別。而在(zai)(zai)凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)應(ying)(ying)(ying)中(zhong)(zhong)(zhong)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)板(ban)釋(shi)放出(chu)(chu)(chu)許多物(wu)質(zhi)，各(ge)凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)因(yin)子(zi)也(ye)(ye)都(dou)(dou)發生(sheng)了變(bian)(bian)化(hua)(hua)。這(zhe)些成(cheng)(cheng)分(fen)(fen)(fen)都(dou)(dou)留在(zai)(zai)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)并(bing)繼續發生(sheng)變(bian)(bian)化(hua)(hua)，如(ru)凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)酶原(yuan)(yuan)(yuan)變(bian)(bian)成(cheng)(cheng)凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)酶，并(bing)隨(sui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)存放時(shi)(shi)間逐漸減少(shao)以(yi)(yi)(yi)(yi)至(zhi)(zhi)(zhi)消失。這(zhe)些也(ye)(ye)都(dou)(dou)是(shi)(shi)(shi)與(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)區(qu)別之(zhi)(zhi)(zhi)處(chu)(chu)(chu)。但大量(liang)未參加凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)反(fan)應(ying)(ying)(ying)的(de)(de)(de)(de)(de)(de)(de)(de)物(wu)質(zhi)則與(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)基(ji)本(ben)相同。為(wei)(wei)避免抗(kang)凝(ning)(ning)(ning)劑(ji)的(de)(de)(de)(de)(de)(de)(de)(de)干擾(rao)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)中(zhong)(zhong)(zhong)許多化(hua)(hua)學成(cheng)(cheng)分(fen)(fen)(fen)的(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)(fen)析(xi)(xi)，都(dou)(dou)以(yi)(yi)(yi)(yi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)為(wei)(wei)樣品(pin)(pin)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)是(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)成(cheng)(cheng)分(fen)(fen)(fen)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)(xi)胞(bao)懸濁于(yu)(yu)(yu)(yu)其(qi)中(zhong)(zhong)(zhong)。人體(ti)(ti)含有2750-3300毫(hao)升血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)，約占血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)總體(ti)(ti)積的(de)(de)(de)(de)(de)(de)(de)(de)55%。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)的(de)(de)(de)(de)(de)(de)(de)(de)絕大部分(fen)(fen)(fen)是(shi)(shi)(shi)水(shui)（體(ti)(ti)積的(de)(de)(de)(de)(de)(de)(de)(de)90%），其(qi)中(zhong)(zhong)(zhong)溶(rong)(rong)(rong)(rong)解(jie)(jie)的(de)(de)(de)(de)(de)(de)(de)(de)物(wu)質(zhi)主(zhu)要是(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)蛋(dan)白(bai)，還包括葡(pu)萄糖、無(wu)(wu)機鹽離(li)(li)子(zi)、激(ji)素以(yi)(yi)(yi)(yi)及二氧化(hua)(hua)碳(tan)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)的(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要功(gong)能是(shi)(shi)(shi)運(yun)載血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)(xi)胞(bao)，同時(shi)(shi)也(ye)(ye)是(shi)(shi)(shi)運(yun)輸(shu)分(fen)(fen)(fen)泌產(chan)物(wu)的(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要媒介。將(jiang)新(xin)鮮血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)離(li)(li)心(xin)，使(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)細(xi)(xi)(xi)胞(bao)沉(chen)降，上(shang)層(ceng)淡(dan)(dan)黃(huang)色(se)清(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)即是(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)與(yu)(yu)(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)區(qu)別是(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)不含纖(xian)維蛋(dan)白(bai)原(yuan)(yuan)(yuan)等凝(ning)(ning)(ning)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)因(yin)子(zi)。
胎(tai)牛(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)(jiang)（去(qu)(qu)(qu)紅細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)、無(wu)(wu)(wu)(wu)菌(jun)過(guo)(guo)濾(lv)(lv)(lv)）產(chan)品(pin)規格(ge)(ge)：100ml/200ml/500ml保(bao)(bao) 存：-20℃，三(san)年。優(you)級新(xin)生(sheng)(sheng)(sheng)牛(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)（無(wu)(wu)(wu)(wu)菌(jun)過(guo)(guo)濾(lv)(lv)(lv)） 使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)時(shi)(shi)常(chang)見問題及解(jie)(jie)(jie)(jie)決方(fang)(fang)法：一(yi)(yi)(yi)(yi)、保(bao)(bao)存血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)法?血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)應(ying)(ying)(ying)保(bao)(bao)存在(zai)(zai)(zai)(zai)-5℃至(zhi)(zhi)(zhi)(zhi)-2O℃若(ruo)(ruo)存放(fang)于4℃時(shi)(shi)，請(qing)勿超過(guo)(guo)一(yi)(yi)(yi)(yi)個月。若(ruo)(ruo)您一(yi)(yi)(yi)(yi)次無(wu)(wu)(wu)(wu)法用(yong)(yong)(yong)(yong)(yong)(yong)完一(yi)(yi)(yi)(yi)瓶(ping)(ping)，建(jian)(jian)議(yi)將(jiang)無(wu)(wu)(wu)(wu)菌(jun)分(fen)裝血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)至(zhi)(zhi)(zhi)(zhi)恰(qia)(qia)當的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)滅(mie)菌(jun)容器內(nei)，再放(fang)回冷(leng)凍(dong)(dong)(dong)(dong)。二、如(ru)(ru)(ru)何(he)解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)才不(bu)(bu)(bu)(bu)會(hui)(hui)使(shi)(shi)(shi)(shi)產(chan)品(pin)質(zhi)(zhi)量(liang)(liang)(liang)受損(sun)?將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)冷(leng)凍(dong)(dong)(dong)(dong)箱取出(chu)后(hou)，先(xian)置于2-8℃冰(bing)箱使(shi)(shi)(shi)(shi)之溶(rong)解(jie)(jie)(jie)(jie)，然(ran)后(hou)在(zai)(zai)(zai)(zai)室(shi)溫(wen)(wen)(wen)下(xia)使(shi)(shi)(shi)(shi)之全溶(rong)。但(dan)(dan)必(bi)須注(zhu)意的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)，溶(rong)解(jie)(jie)(jie)(jie)過(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)必(bi)須規則(ze)地搖晃(huang)(huang)均(jun)勻。三(san)、如(ru)(ru)(ru)何(he)避免(mian)(mian)沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)生(sheng)(sheng)(sheng)?1.解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)，請(qing)按照(zhao)所建(jian)(jian)議(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)逐(zhu)步(bu)解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)法(-2O℃至(zhi)(zhi)(zhi)(zhi)4℃至(zhi)(zhi)(zhi)(zhi)室(shi)溫(wen)(wen)(wen))，若(ruo)(ruo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)時(shi)(shi)改變(bian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)溫(wen)(wen)(wen)度(du)太大(da)(如(ru)(ru)(ru)-20℃至(zhi)(zhi)(zhi)(zhi)37℃)，實(shi)驗(yan)顯(xian)示非(fei)常(chang)容易(yi)產(chan)生(sheng)(sheng)(sheng)沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)(wu)。2.解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)，請(qing)隨(sui)時(shi)(shi)將(jiang)之搖晃(huang)(huang)均(jun)勻，使(shi)(shi)(shi)(shi)溫(wen)(wen)(wen)度(du)及成(cheng)(cheng)(cheng)分(fen)均(jun)一(yi)(yi)(yi)(yi)，減少沉(chen)(chen)淀(dian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發(fa)(fa)生(sheng)(sheng)(sheng)3.請(qing)勿將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)置于37℃太久。若(ruo)(ruo)在(zai)(zai)(zai)(zai)37℃放(fang)置太久，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)會(hui)(hui)變(bian)得混濁(zhuo)，同時(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)許多(duo)(duo)較不(bu)(bu)(bu)(bu)穩定的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)分(fen)也會(hui)(hui)因(yin)(yin)此(ci)受到(dao)損(sun)害，而影響(xiang)(xiang)(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)量(liang)(liang)(liang)。4.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱(re)滅(mie)活非(fei)常(chang)容易(yi)造(zao)(zao)成(cheng)(cheng)(cheng)沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)增多(duo)(duo)，若(ruo)(ruo)非(fei)必(bi)要(yao)(yao)(yao)(yao)，可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)無(wu)(wu)(wu)(wu)須做(zuo)此(ci)步(bu)驟。5.若(ruo)(ruo)必(bi)須做(zuo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱(re)滅(mie)活，請(qing)遵守56℃，30分(fen)鐘(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)(yuan)則(ze)，并(bing)且隨(sui)時(shi)(shi)搖晃(huang)(huang)均(jun)勻。溫(wen)(wen)(wen)度(du)過(guo)(guo)高，時(shi)(shi)間過(guo)(guo)久或(huo)搖晃(huang)(huang)不(bu)(bu)(bu)(bu)均(jun)勻，都會(hui)(hui)造(zao)(zao)成(cheng)(cheng)(cheng)沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)增多(duo)(duo)。四、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)后(hou)發(fa)(fa)現有(you)(you)絮(xu)(xu)狀沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)(wu)出(chu)現，該如(ru)(ru)(ru)何(he)處(chu)理(li)(li)? 欲去(qu)(qu)(qu)除這(zhe)些(xie)絮(xu)(xu)狀沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)(wu)，可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)分(fen)裝至(zhi)(zhi)(zhi)(zhi)無(wu)(wu)(wu)(wu)菌(jun)離(li)心管(guan)內(nei)，以(yi)(yi)(yi)400g稍微(wei)離(li)心，上清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)即可(ke)(ke)(ke)(ke)接著加(jia)(jia)入培(pei)養(yang)(yang)基內(nei)一(yi)(yi)(yi)(yi)起(qi)過(guo)(guo)濾(lv)(lv)(lv)。但(dan)(dan)不(bu)(bu)(bu)(bu)建(jian)(jian)議(yi)以(yi)(yi)(yi)過(guo)(guo)濾(lv)(lv)(lv)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)法去(qu)(qu)(qu)除這(zhe)些(xie)絮(xu)(xu)狀沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)(wu)，因(yin)(yin)為(wei)(wei)它可(ke)(ke)(ke)(ke)能(neng)會(hui)(hui)阻(zu)塞過(guo)(guo)濾(lv)(lv)(lv)膜(mo)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)注(zhu)意事(shi)項：防(fang)(fang)止發(fa)(fa)病免(mian)(mian)疫血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)多(duo)(duo)用(yong)(yong)(yong)(yong)(yong)(yong)馬、牛(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)制(zhi)(zhi)(zhi)備，馬、牛(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)對(dui)其它動物(wu)(wu)(wu)(wu)(wu)來(lai)說(shuo)，也具有(you)(you)抗原(yuan)(yuan)性(xing)，有(you)(you)引起(qi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)可(ke)(ke)(ke)(ke)能(neng)，因(yin)(yin)此(ci)，使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)免(mian)(mian)疫血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)要(yao)(yao)(yao)(yao)注(zhu)意防(fang)(fang)止引起(qi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)病，預防(fang)(fang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)(zhu)要(yao)(yao)(yao)(yao)措施是(shi)(shi)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)提純的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)(zhi)(zhi)品(pin)，不(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)合(he)格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)品(pin)；另外，不(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)有(you)(you)急功近利的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)想法，要(yao)(yao)(yao)(yao)按照(zhao)要(yao)(yao)(yao)(yao)求劑量(liang)(liang)(liang)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)，一(yi)(yi)(yi)(yi)次用(yong)(yong)(yong)(yong)(yong)(yong)量(liang)(liang)(liang)不(bu)(bu)(bu)(bu)可(ke)(ke)(ke)(ke)過(guo)(guo)大(da)。Tips：1. 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)存：在(zai)(zai)(zai)(zai)-15℃以(yi)(yi)(yi)下(xia)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)效期為(wei)(wei)5年，4℃可(ke)(ke)(ke)(ke)保(bao)(bao)存數周(zhou)，不(bu)(bu)(bu)(bu)宜室(shi)溫(wen)(wen)(wen)保(bao)(bao)存，不(bu)(bu)(bu)(bu)宜反復(fu)凍(dong)(dong)(dong)(dong)融。配制(zhi)(zhi)(zhi)成(cheng)(cheng)(cheng)完全培(pei)養(yang)(yang)基后(hou)應(ying)(ying)(ying)在(zai)(zai)(zai)(zai)四周(zhou)內(nei)用(yong)(yong)(yong)(yong)(yong)(yong)完，一(yi)(yi)(yi)(yi)瓶(ping)(ping)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)如(ru)(ru)(ru)無(wu)(wu)(wu)(wu)法一(yi)(yi)(yi)(yi)次用(yong)(yong)(yong)(yong)(yong)(yong)完應(ying)(ying)(ying)無(wu)(wu)(wu)(wu)菌(jun)分(fen)裝成(cheng)(cheng)(cheng)恰(qia)(qia)當數量(liang)(liang)(liang)并(bing)冷(leng)凍(dong)(dong)(dong)(dong)保(bao)(bao)存。2、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)絮(xu)(xu)狀沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)(wu)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)多(duo)(duo)種(zhong)蛋(dan)(dan)白(bai)(bai)質(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)物(wu)(wu)(wu)(wu)(wu)，在(zai)(zai)(zai)(zai)凍(dong)(dong)(dong)(dong)融過(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)會(hui)(hui)出(chu)現絮(xu)(xu)狀沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)(wu)，主(zhu)(zhu)要(yao)(yao)(yao)(yao)原(yuan)(yuan)因(yin)(yin)是(shi)(shi)由(you)于血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)脂蛋(dan)(dan)白(bai)(bai)和(he)纖(xian)維蛋(dan)(dan)白(bai)(bai)變(bian)性(xing)所造(zao)(zao)成(cheng)(cheng)(cheng)。但(dan)(dan)并(bing)不(bu)(bu)(bu)(bu)影響(xiang)(xiang)(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)本身的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)量(liang)(liang)(liang)和(he)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)。如(ru)(ru)(ru)需(xu)去(qu)(qu)(qu)除可(ke)(ke)(ke)(ke)先(xian)400g離(li)心取上清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)配制(zhi)(zhi)(zhi)成(cheng)(cheng)(cheng)完全培(pei)養(yang)(yang)基再過(guo)(guo)濾(lv)(lv)(lv)即可(ke)(ke)(ke)(ke)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)。3、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)：合(he)理(li)(li)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)方(fang)(fang)式可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)更好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)護血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)量(liang)(liang)(liang)并(bing)可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)在(zai)(zai)(zai)(zai)＊大(da)程(cheng)度(du)上減少絮(xu)(xu)狀沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)生(sheng)(sheng)(sheng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)從(cong)低(di)溫(wen)(wen)(wen)冰(bing)箱中(zhong)(zhong)(zhong)取出(chu)后(hou)，經(jing)4℃冰(bing)箱、室(shi)溫(wen)(wen)(wen)、37℃水浴(yu)逐(zhu)步(bu)規則(ze)搖晃(huang)(huang)均(jun)勻融解(jie)(jie)(jie)(jie)，直(zhi)至(zhi)(zhi)(zhi)(zhi)完全融解(jie)(jie)(jie)(jie)后(hou)再使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)。4、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱(re)滅(mie)活：將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)嚴(yan)格(ge)(ge)56℃水浴(yu)均(jun)勻加(jia)(jia)溫(wen)(wen)(wen)30分(fen)鐘(zhong)(zhong)可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)滅(mie)活血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補體(ti)系統。是(shi)(shi)不(bu)(bu)(bu)(bu)是(shi)(shi)所有(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)途的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)都需(xu)要(yao)(yao)(yao)(yao)熱(re)滅(mie)活呢？對(dui)大(da)多(duo)(duo)數細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)來(lai)說(shuo)是(shi)(shi)不(bu)(bu)(bu)(bu)需(xu)要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。激(ji)(ji)活的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補體(ti)能(neng)參(can)與溶(rong)解(jie)(jie)(jie)(jie)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)，刺激(ji)(ji)光滑(hua)肌收(shou)縮，細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)和(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小板釋放(fang)組(zu)胺，激(ji)(ji)活淋巴細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)和(he)巨(ju)噬細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)。因(yin)(yin)此(ci)在(zai)(zai)(zai)(zai)免(mian)(mian)疫學研(yan)究(jiu)，培(pei)養(yang)(yang)ES細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)，昆蟲細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)和(he)平滑(hua)肌肉(rou)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)時(shi)(shi)，建(jian)(jian)議(yi)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)熱(re)滅(mie)活血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)，在(zai)(zai)(zai)(zai)正(zheng)常(chang)情況下(xia)加(jia)(jia)熱(re)處(chu)理(li)(li)對(dui)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)促細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)長效應(ying)(ying)(ying)無(wu)(wu)(wu)(wu)明顯(xian)促進(jin)作用(yong)(yong)(yong)(yong)(yong)(yong)，甚(shen)至(zhi)(zhi)(zhi)(zhi)會(hui)(hui)因(yin)(yin)為(wei)(wei)加(jia)(jia)熱(re)處(chu)理(li)(li)影響(xiang)(xiang)(xiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)量(liang)(liang)(liang)。5、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)“小黑點”現象：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)經(jing)過(guo)(guo)一(yi)(yi)(yi)(yi)段時(shi)(shi)間的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低(di)溫(wen)(wen)(wen)凍(dong)(dong)(dong)(dong)存再融解(jie)(jie)(jie)(jie)后(hou)，在(zai)(zai)(zai)(zai)正(zheng)常(chang)情況下(xia)會(hui)(hui)出(chu)現絮(xu)(xu)狀蛋(dan)(dan)白(bai)(bai)沉(chen)(chen)淀(dian)。但(dan)(dan)往往有(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)戶反應(ying)(ying)(ying)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)有(you)(you)“小黑點”現象，認(ren)為(wei)(wei)是(shi)(shi)微(wei)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)污染(ran)。實(shi)驗(yan)發(fa)(fa)現血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)37℃環境放(fang)置時(shi)(shi)間過(guo)(guo)長或(huo)經(jing)過(guo)(guo)加(jia)(jia)熱(re)滅(mie)活處(chu)理(li)(li)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)(wu)往往會(hui)(hui)呈增多(duo)(duo)趨(qu)勢，這(zhe)些(xie)沉(chen)(chen)淀(dian)物(wu)(wu)(wu)(wu)(wu)在(zai)(zai)(zai)(zai)倒置顯(xian)微(wei)鏡下(xia)觀察，像是(shi)(shi)“小黑點”，讓使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)者(zhe)誤(wu)以(yi)(yi)(yi)為(wei)(wei)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)遭受微(wei)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)污染(ran)并(bing)在(zai)(zai)(zai)(zai)分(fen)裂(lie)增殖。因(yin)(yin)此(ci)如(ru)(ru)(ru)無(wu)(wu)(wu)(wu)必(bi)要(yao)(yao)(yao)(yao)，完全可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)不(bu)(bu)(bu)(bu)進(jin)行熱(re)滅(mie)活處(chu)理(li)(li)并(bing)保(bao)(bao)證(zheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低(di)溫(wen)(wen)(wen)保(bao)(bao)存。實(shi)驗(yan)證(zheng)明使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)注(zhu)意以(yi)(yi)(yi)下(xia)幾點事(shi)項不(bu)(bu)(bu)(bu)但(dan)(dan)可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)保(bao)(bao)證(zheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)量(liang)(liang)(liang)并(bing)能(neng)給(gei)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)者(zhe)帶來(lai)方(fang)(fang)便：◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)(ke)(ke)在(zai)(zai)(zai)(zai)-15℃以(yi)(yi)(yi)下(xia)低(di)溫(wen)(wen)(wen)保(bao)(bao)存。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)(yi)(yi)(yi)瓶(ping)(ping)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)一(yi)(yi)(yi)(yi)次無(wu)(wu)(wu)(wu)法用(yong)(yong)(yong)(yong)(yong)(yong)完的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)(ying)(ying)分(fen)裝凍(dong)(dong)(dong)(dong)存，分(fen)次用(yong)(yong)(yong)(yong)(yong)(yong)完。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)按-15℃以(yi)(yi)(yi)下(xia)至(zhi)(zhi)(zhi)(zhi)4℃至(zhi)(zhi)(zhi)(zhi)室(shi)溫(wen)(wen)(wen)至(zhi)(zhi)(zhi)(zhi)37℃逐(zhu)步(bu)均(jun)勻解(jie)(jie)(jie)(jie)凍(dong)(dong)(dong)(dong)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)。◎若(ruo)(ruo)非(fei)必(bi)要(yao)(yao)(yao)(yao)，無(wu)(wu)(wu)(wu)須進(jin)行熱(re)滅(mie)活。若(ruo)(ruo)必(bi)須做(zuo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)熱(re)滅(mie)活的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，須嚴(yan)格(ge)(ge)控(kong)制(zhi)(zhi)(zhi)在(zai)(zai)(zai)(zai)56℃水浴(yu)30分(fen)鐘(zhong)(zhong)均(jun)勻加(jia)(jia)熱(re)滅(mie)活。胎(tai)牛(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)(jiang)（去(qu)(qu)(qu)紅細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)、無(wu)(wu)(wu)(wu)菌(jun)過(guo)(guo)濾(lv)(lv)(lv)） 主(zhu)(zhu)要(yao)(yao)(yao)(yao)成(cheng)(cheng)(cheng)分(fen)： 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)由(you)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)(jiang)去(qu)(qu)(qu)除纖(xian)維蛋(dan)(dan)白(bai)(bai)原(yuan)(yuan)而形成(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)種(zhong)很復(fu)雜(za)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混合(he)物(wu)(wu)(wu)(wu)(wu)，其組(zu)成(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)份雖(sui)大(da)部分(fen)已知，但(dan)(dan)還有(you)(you)一(yi)(yi)(yi)(yi)部分(fen)尚(shang)不(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)楚，且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)組(zu)成(cheng)(cheng)(cheng)及含(han)量(liang)(liang)(liang)常(chang)隨(sui)供血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動物(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性(xing)別、年齡、生(sheng)(sheng)(sheng)li條件和(he)營養(yang)(yang)條件不(bu)(bu)(bu)(bu)同而異(yi)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)(jiang)中(zhong)(zhong)(zhong)不(bu)(bu)(bu)(bu)含(han)纖(xian)維蛋(dan)(dan)白(bai)(bai)原(yuan)(yuan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠狀液(ye)體(ti)，具有(you)(you)維持血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正(zheng)常(chang)粘度(du)、酸堿度(du)、滲透壓等作用(yong)(yong)(yong)(yong)(yong)(yong)。它主(zhu)(zhu)要(yao)(yao)(yao)(yao)由(you)水和(he)各(ge)種(zhong)化學成(cheng)(cheng)(cheng)分(fen)組(zu)成(cheng)(cheng)(cheng)，這(zhe)些(xie)化學成(cheng)(cheng)(cheng)分(fen)包括白(bai)(bai)蛋(dan)(dan)白(bai)(bai)、α1、α2、β、γ-球蛋(dan)(dan)白(bai)(bai)，甘油三(san)酯，總膽(dan)固醇，谷丙轉(zhuan)氨酶等。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)含(han)有(you)(you)各(ge)種(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿(jiang)(jiang)(jiang)(jiang)(jiang)蛋(dan)(dan)白(bai)(bai)、多(duo)(duo)肽、脂肪、碳(tan)水化合(he)物(wu)(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)長因(yin)(yin)子、激(ji)(ji)素(su)、無(wu)(wu)(wu)(wu)機物(wu)(wu)(wu)(wu)(wu)等，這(zhe)些(xie)物(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)對(dui)促進(jin)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)長或(huo)抑制(zhi)(zhi)(zhi)生(sheng)(sheng)(sheng)長活性(xing)是(shi)(shi)達到(dao)生(sheng)(sheng)(sheng)li平衡(heng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。
大(da)(da)牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿（去(qu)紅細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)、無(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)過(guo)(guo)(guo)濾(lv)）產(chan)(chan)品(pin)規(gui)格：100ml/200ml/500ml保(bao)(bao) 存(cun)：-20℃，三(san)年。 使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)時(shi)(shi)(shi)(shi)(shi)常見問(wen)題(ti)及(ji)解(jie)(jie)(jie)(jie)(jie)(jie)決方(fang)(fang)法(fa)：一(yi)(yi)(yi)(yi)(yi)、保(bao)(bao)存(cun)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)法(fa)?血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)應(ying)(ying)(ying)(ying)保(bao)(bao)存(cun)在(zai)(zai)(zai)(zai)-5℃至(zhi)(zhi)(zhi)-2O℃若(ruo)存(cun)放于(yu)4℃時(shi)(shi)(shi)(shi)(shi)，請勿超過(guo)(guo)(guo)一(yi)(yi)(yi)(yi)(yi)個(ge)月(yue)(yue)。若(ruo)您一(yi)(yi)(yi)(yi)(yi)次無(wu)(wu)(wu)(wu)法(fa)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)一(yi)(yi)(yi)(yi)(yi)瓶(ping)(ping)，建(jian)議將(jiang)(jiang)(jiang)無(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)分(fen)(fen)(fen)裝(zhuang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)至(zhi)(zhi)(zhi)恰當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)滅(mie)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)容(rong)(rong)器(qi)內(nei)，再(zai)放回冷(leng)(leng)凍(dong)(dong)。二(er)、如(ru)(ru)(ru)何(he)解(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)才不(bu)(bu)(bu)(bu)(bu)會(hui)(hui)使(shi)(shi)(shi)(shi)產(chan)(chan)品(pin)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)受損(sun)(sun)?將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)從冷(leng)(leng)凍(dong)(dong)箱取出(chu)(chu)后(hou)(hou)(hou)，先(xian)置(zhi)(zhi)于(yu)2-8℃冰箱使(shi)(shi)(shi)(shi)之溶解(jie)(jie)(jie)(jie)(jie)(jie)，然(ran)后(hou)(hou)(hou)在(zai)(zai)(zai)(zai)室(shi)(shi)溫(wen)下使(shi)(shi)(shi)(shi)之全(quan)溶。但(dan)(dan)必(bi)須(xu)(xu)注意(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)，溶解(jie)(jie)(jie)(jie)(jie)(jie)過(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)必(bi)須(xu)(xu)規(gui)則(ze)(ze)地搖晃均(jun)(jun)勻(yun)。三(san)、如(ru)(ru)(ru)何(he)避免(mian)沉(chen)(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)(chan)生(sheng)(sheng)(sheng)(sheng)?1.解(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)(shi)(shi)(shi)，請按(an)(an)(an)照所(suo)建(jian)議的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)逐(zhu)步(bu)解(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)法(fa)(-2O℃至(zhi)(zhi)(zhi)4℃至(zhi)(zhi)(zhi)室(shi)(shi)溫(wen))，若(ruo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)時(shi)(shi)(shi)(shi)(shi)改變(bian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)溫(wen)度(du)太大(da)(da)(如(ru)(ru)(ru)-20℃至(zhi)(zhi)(zhi)37℃)，實驗顯示非(fei)常容(rong)(rong)易產(chan)(chan)生(sheng)(sheng)(sheng)(sheng)沉(chen)(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)。2.解(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)(shi)(shi)(shi)，請隨(sui)時(shi)(shi)(shi)(shi)(shi)將(jiang)(jiang)(jiang)之搖晃均(jun)(jun)勻(yun)，使(shi)(shi)(shi)(shi)溫(wen)度(du)及(ji)成(cheng)(cheng)(cheng)分(fen)(fen)(fen)均(jun)(jun)一(yi)(yi)(yi)(yi)(yi)，減少沉(chen)(chen)淀(dian)(dian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)發生(sheng)(sheng)(sheng)(sheng)3.請勿將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)置(zhi)(zhi)于(yu)37℃太久。若(ruo)在(zai)(zai)(zai)(zai)37℃放置(zhi)(zhi)太久，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)會(hui)(hui)變(bian)得混(hun)濁，同時(shi)(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)許多較不(bu)(bu)(bu)(bu)(bu)穩(wen)定(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)成(cheng)(cheng)(cheng)分(fen)(fen)(fen)也會(hui)(hui)因(yin)此(ci)受到損(sun)(sun)害，而影(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)。4.血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱(re)滅(mie)活(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)非(fei)常容(rong)(rong)易造成(cheng)(cheng)(cheng)沉(chen)(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)增(zeng)多，若(ruo)非(fei)必(bi)要(yao)，可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)無(wu)(wu)(wu)(wu)須(xu)(xu)做(zuo)此(ci)步(bu)驟。5.若(ruo)必(bi)須(xu)(xu)做(zuo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱(re)滅(mie)活(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)，請遵守56℃，30分(fen)(fen)(fen)鐘(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)原(yuan)(yuan)則(ze)(ze)，并(bing)(bing)(bing)且隨(sui)時(shi)(shi)(shi)(shi)(shi)搖晃均(jun)(jun)勻(yun)。溫(wen)度(du)過(guo)(guo)(guo)高，時(shi)(shi)(shi)(shi)(shi)間過(guo)(guo)(guo)久或(huo)搖晃不(bu)(bu)(bu)(bu)(bu)均(jun)(jun)勻(yun)，都(dou)會(hui)(hui)造成(cheng)(cheng)(cheng)沉(chen)(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)增(zeng)多。四、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)解(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)后(hou)(hou)(hou)發現(xian)(xian)(xian)(xian)有(you)(you)絮(xu)狀沉(chen)(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)出(chu)(chu)現(xian)(xian)(xian)(xian)，該如(ru)(ru)(ru)何(he)處(chu)(chu)理(li)? 欲去(qu)除(chu)(chu)這些(xie)(xie)絮(xu)狀沉(chen)(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)，可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)分(fen)(fen)(fen)裝(zhuang)至(zhi)(zhi)(zhi)無(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)離(li)(li)心管內(nei)，以(yi)(yi)(yi)(yi)(yi)400g稍微(wei)(wei)(wei)離(li)(li)心，上(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)即可(ke)(ke)(ke)(ke)接(jie)(jie)(jie)著加(jia)入(ru)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)內(nei)一(yi)(yi)(yi)(yi)(yi)起(qi)(qi)(qi)過(guo)(guo)(guo)濾(lv)。但(dan)(dan)不(bu)(bu)(bu)(bu)(bu)建(jian)議以(yi)(yi)(yi)(yi)(yi)過(guo)(guo)(guo)濾(lv)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)法(fa)去(qu)除(chu)(chu)這些(xie)(xie)絮(xu)狀沉(chen)(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)，因(yin)為(wei)(wei)(wei)它(ta)(ta)可(ke)(ke)(ke)(ke)能會(hui)(hui)阻(zu)塞(sai)(sai)過(guo)(guo)(guo)濾(lv)膜。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)注意(yi)(yi)事項：防(fang)止(zhi)(zhi)發病(bing)免(mian)疫血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)多用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)馬、牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)制(zhi)(zhi)備(bei)(bei)，馬、牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)對其它(ta)(ta)動物(wu)(wu)(wu)(wu)來說(shuo)，也具有(you)(you)抗原(yuan)(yuan)性，有(you)(you)引起(qi)(qi)(qi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)病(bing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)可(ke)(ke)(ke)(ke)能，因(yin)此(ci)，使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)免(mian)疫血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)要(yao)注意(yi)(yi)防(fang)止(zhi)(zhi)引起(qi)(qi)(qi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)病(bing)，預(yu)防(fang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要(yao)措施是(shi)(shi)(shi)(shi)(shi)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)提(ti)純(chun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)(zhi)品(pin)，不(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)合(he)(he)格的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)(chan)品(pin)；另外，不(bu)(bu)(bu)(bu)(bu)要(yao)有(you)(you)急(ji)功(gong)近利的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)想法(fa)，要(yao)按(an)(an)(an)照要(yao)求(qiu)劑量(liang)(liang)(liang)(liang)(liang)(liang)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，一(yi)(yi)(yi)(yi)(yi)次用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)量(liang)(liang)(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)(bu)可(ke)(ke)(ke)(ke)過(guo)(guo)(guo)大(da)(da)。Tips：1. 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)存(cun)：在(zai)(zai)(zai)(zai)-15℃以(yi)(yi)(yi)(yi)(yi)下血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)有(you)(you)效期為(wei)(wei)(wei)5年，4℃可(ke)(ke)(ke)(ke)保(bao)(bao)存(cun)數周(zhou)，不(bu)(bu)(bu)(bu)(bu)宜室(shi)(shi)溫(wen)保(bao)(bao)存(cun)，不(bu)(bu)(bu)(bu)(bu)宜反(fan)復(fu)凍(dong)(dong)融。配(pei)(pei)(pei)(pei)制(zhi)(zhi)成(cheng)(cheng)(cheng)完(wan)全(quan)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)后(hou)(hou)(hou)應(ying)(ying)(ying)(ying)在(zai)(zai)(zai)(zai)四周(zhou)內(nei)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)，一(yi)(yi)(yi)(yi)(yi)瓶(ping)(ping)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)如(ru)(ru)(ru)無(wu)(wu)(wu)(wu)法(fa)一(yi)(yi)(yi)(yi)(yi)次用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)應(ying)(ying)(ying)(ying)無(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)分(fen)(fen)(fen)裝(zhuang)成(cheng)(cheng)(cheng)恰當(dang)數量(liang)(liang)(liang)(liang)(liang)(liang)并(bing)(bing)(bing)冷(leng)(leng)凍(dong)(dong)保(bao)(bao)存(cun)。2、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)絮(xu)狀沉(chen)(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)多種(zhong)(zhong)(zhong)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)質(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混(hun)合(he)(he)物(wu)(wu)(wu)(wu)，在(zai)(zai)(zai)(zai)凍(dong)(dong)融過(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)會(hui)(hui)出(chu)(chu)現(xian)(xian)(xian)(xian)絮(xu)狀沉(chen)(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)，主(zhu)要(yao)原(yuan)(yuan)因(yin)是(shi)(shi)(shi)(shi)(shi)由(you)于(yu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)脂(zhi)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)和(he)(he)(he)纖維(wei)(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)變(bian)性所(suo)造成(cheng)(cheng)(cheng)。但(dan)(dan)并(bing)(bing)(bing)不(bu)(bu)(bu)(bu)(bu)影(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)本(ben)(ben)身的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。如(ru)(ru)(ru)需(xu)(xu)去(qu)除(chu)(chu)可(ke)(ke)(ke)(ke)先(xian)400g離(li)(li)心取上(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)配(pei)(pei)(pei)(pei)制(zhi)(zhi)成(cheng)(cheng)(cheng)完(wan)全(quan)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)再(zai)過(guo)(guo)(guo)濾(lv)即可(ke)(ke)(ke)(ke)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。3、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)：合(he)(he)理(li)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)解(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)方(fang)(fang)式可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)更好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)護血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)并(bing)(bing)(bing)可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)在(zai)(zai)(zai)(zai)＊大(da)(da)程度(du)上(shang)減少絮(xu)狀沉(chen)(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)產(chan)(chan)生(sheng)(sheng)(sheng)(sheng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)從低溫(wen)冰箱中(zhong)(zhong)(zhong)(zhong)取出(chu)(chu)后(hou)(hou)(hou)，經(jing)(jing)4℃冰箱、室(shi)(shi)溫(wen)、37℃水(shui)(shui)(shui)浴逐(zhu)步(bu)規(gui)則(ze)(ze)搖晃均(jun)(jun)勻(yun)融解(jie)(jie)(jie)(jie)(jie)(jie)，直至(zhi)(zhi)(zhi)完(wan)全(quan)融解(jie)(jie)(jie)(jie)(jie)(jie)后(hou)(hou)(hou)再(zai)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。4、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熱(re)滅(mie)活(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)：將(jiang)(jiang)(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)嚴格56℃水(shui)(shui)(shui)浴均(jun)(jun)勻(yun)加(jia)溫(wen)30分(fen)(fen)(fen)鐘(zhong)可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)滅(mie)活(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補(bu)體(ti)(ti)系統。是(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)是(shi)(shi)(shi)(shi)(shi)所(suo)有(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)途的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)都(dou)需(xu)(xu)要(yao)熱(re)滅(mie)活(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)呢？對大(da)(da)多數細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)來說(shuo)是(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)需(xu)(xu)要(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。激(ji)(ji)(ji)活(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)補(bu)體(ti)(ti)能參與溶解(jie)(jie)(jie)(jie)(jie)(jie)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)，刺激(ji)(ji)(ji)光(guang)(guang)滑(hua)肌(ji)收縮，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)和(he)(he)(he)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)板釋放組胺，激(ji)(ji)(ji)活(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)淋巴細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)和(he)(he)(he)巨(ju)噬細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)。因(yin)此(ci)在(zai)(zai)(zai)(zai)免(mian)疫學(xue)(xue)研究，培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)ES細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)，昆蟲細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)和(he)(he)(he)平滑(hua)肌(ji)肉細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)時(shi)(shi)(shi)(shi)(shi)，建(jian)議使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)熱(re)滅(mie)活(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)，在(zai)(zai)(zai)(zai)正常情況下加(jia)熱(re)處(chu)(chu)理(li)對血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)促(cu)(cu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長效應(ying)(ying)(ying)(ying)無(wu)(wu)(wu)(wu)明(ming)顯促(cu)(cu)進(jin)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，甚至(zhi)(zhi)(zhi)會(hui)(hui)因(yin)為(wei)(wei)(wei)加(jia)熱(re)處(chu)(chu)理(li)影(ying)響血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)。5、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)“小(xiao)黑(hei)點(dian)(dian)”現(xian)(xian)(xian)(xian)象：血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)經(jing)(jing)過(guo)(guo)(guo)一(yi)(yi)(yi)(yi)(yi)段(duan)時(shi)(shi)(shi)(shi)(shi)間的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低溫(wen)凍(dong)(dong)存(cun)再(zai)融解(jie)(jie)(jie)(jie)(jie)(jie)后(hou)(hou)(hou)，在(zai)(zai)(zai)(zai)正常情況下會(hui)(hui)出(chu)(chu)現(xian)(xian)(xian)(xian)絮(xu)狀蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)沉(chen)(chen)淀(dian)(dian)。但(dan)(dan)往(wang)往(wang)有(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶反(fan)應(ying)(ying)(ying)(ying)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)有(you)(you)“小(xiao)黑(hei)點(dian)(dian)”現(xian)(xian)(xian)(xian)象，認(ren)為(wei)(wei)(wei)是(shi)(shi)(shi)(shi)(shi)微(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)污(wu)(wu)染。實驗發現(xian)(xian)(xian)(xian)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)在(zai)(zai)(zai)(zai)37℃環(huan)境放置(zhi)(zhi)時(shi)(shi)(shi)(shi)(shi)間過(guo)(guo)(guo)長或(huo)經(jing)(jing)過(guo)(guo)(guo)加(jia)熱(re)滅(mie)活(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)處(chu)(chu)理(li)，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)沉(chen)(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)往(wang)往(wang)會(hui)(hui)呈增(zeng)多趨勢，這些(xie)(xie)沉(chen)(chen)淀(dian)(dian)物(wu)(wu)(wu)(wu)在(zai)(zai)(zai)(zai)倒(dao)置(zhi)(zhi)顯微(wei)(wei)(wei)鏡下觀察，像是(shi)(shi)(shi)(shi)(shi)“小(xiao)黑(hei)點(dian)(dian)”，讓使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)者(zhe)誤(wu)以(yi)(yi)(yi)(yi)(yi)為(wei)(wei)(wei)是(shi)(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)遭受微(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)污(wu)(wu)染并(bing)(bing)(bing)在(zai)(zai)(zai)(zai)分(fen)(fen)(fen)裂增(zeng)殖。因(yin)此(ci)如(ru)(ru)(ru)無(wu)(wu)(wu)(wu)必(bi)要(yao)，完(wan)全(quan)可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)不(bu)(bu)(bu)(bu)(bu)進(jin)行熱(re)滅(mie)活(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)處(chu)(chu)理(li)并(bing)(bing)(bing)保(bao)(bao)證(zheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低溫(wen)保(bao)(bao)存(cun)。實驗證(zheng)明(ming)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)時(shi)(shi)(shi)(shi)(shi)注意(yi)(yi)以(yi)(yi)(yi)(yi)(yi)下幾點(dian)(dian)事項不(bu)(bu)(bu)(bu)(bu)但(dan)(dan)可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)保(bao)(bao)證(zheng)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)并(bing)(bing)(bing)能給使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)者(zhe)帶來方(fang)(fang)便：◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)可(ke)(ke)(ke)(ke)在(zai)(zai)(zai)(zai)-15℃以(yi)(yi)(yi)(yi)(yi)下低溫(wen)保(bao)(bao)存(cun)。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)一(yi)(yi)(yi)(yi)(yi)瓶(ping)(ping)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)一(yi)(yi)(yi)(yi)(yi)次無(wu)(wu)(wu)(wu)法(fa)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)(ying)(ying)(ying)分(fen)(fen)(fen)裝(zhuang)凍(dong)(dong)存(cun)，分(fen)(fen)(fen)次用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)完(wan)。◎血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)按(an)(an)(an)-15℃以(yi)(yi)(yi)(yi)(yi)下至(zhi)(zhi)(zhi)4℃至(zhi)(zhi)(zhi)室(shi)(shi)溫(wen)至(zhi)(zhi)(zhi)37℃逐(zhu)步(bu)均(jun)(jun)勻(yun)解(jie)(jie)(jie)(jie)(jie)(jie)凍(dong)(dong)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。◎若(ruo)非(fei)必(bi)要(yao)，無(wu)(wu)(wu)(wu)須(xu)(xu)進(jin)行熱(re)滅(mie)活(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)。若(ruo)必(bi)須(xu)(xu)做(zuo)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)熱(re)滅(mie)活(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，須(xu)(xu)嚴格控制(zhi)(zhi)在(zai)(zai)(zai)(zai)56℃水(shui)(shui)(shui)浴30分(fen)(fen)(fen)鐘(zhong)均(jun)(jun)勻(yun)加(jia)熱(re)滅(mie)活(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)。大(da)(da)牛(niu)(niu)(niu)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿（去(qu)紅細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)、無(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)過(guo)(guo)(guo)濾(lv)）主(zhu)要(yao)成(cheng)(cheng)(cheng)分(fen)(fen)(fen)： 血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)由(you)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿去(qu)除(chu)(chu)纖維(wei)(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)而形成(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)種(zhong)(zhong)(zhong)很復(fu)雜的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)混(hun)合(he)(he)物(wu)(wu)(wu)(wu)，其組成(cheng)(cheng)(cheng)成(cheng)(cheng)(cheng)份雖(sui)大(da)(da)部分(fen)(fen)(fen)已知，但(dan)(dan)還有(you)(you)一(yi)(yi)(yi)(yi)(yi)部分(fen)(fen)(fen)尚不(bu)(bu)(bu)(bu)(bu)清(qing)(qing)(qing)(qing)(qing)(qing)楚，且血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)組成(cheng)(cheng)(cheng)及(ji)含(han)量(liang)(liang)(liang)(liang)(liang)(liang)常隨(sui)供(gong)(gong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)動物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)性別(bie)(bie)、年齡(ling)、生(sheng)(sheng)(sheng)(sheng)li條(tiao)件(jian)和(he)(he)(he)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)條(tiao)件(jian)不(bu)(bu)(bu)(bu)(bu)同而異(yi)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)是(shi)(shi)(shi)(shi)(shi)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿中(zhong)(zhong)(zhong)(zhong)不(bu)(bu)(bu)(bu)(bu)含(han)纖維(wei)(wei)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)原(yuan)(yuan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)膠狀液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)，具有(you)(you)維(wei)(wei)持血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)正常粘(zhan)度(du)、酸(suan)堿度(du)、滲透(tou)壓等(deng)(deng)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。它(ta)(ta)主(zhu)要(yao)由(you)水(shui)(shui)(shui)和(he)(he)(he)各種(zhong)(zhong)(zhong)化(hua)學(xue)(xue)成(cheng)(cheng)(cheng)分(fen)(fen)(fen)組成(cheng)(cheng)(cheng)，這些(xie)(xie)化(hua)學(xue)(xue)成(cheng)(cheng)(cheng)分(fen)(fen)(fen)包括白(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)、α1、α2、β、γ-球蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)，甘(gan)油(you)三(san)酯，總膽固(gu)醇(chun)，谷丙(bing)轉氨酶等(deng)(deng)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)含(han)有(you)(you)各種(zhong)(zhong)(zhong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)漿蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)、多肽(tai)、脂(zhi)肪(fang)、碳水(shui)(shui)(shui)化(hua)合(he)(he)物(wu)(wu)(wu)(wu)、生(sheng)(sheng)(sheng)(sheng)長因(yin)子(zi)、激(ji)(ji)(ji)素(su)、無(wu)(wu)(wu)(wu)機(ji)物(wu)(wu)(wu)(wu)等(deng)(deng)，這些(xie)(xie)物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)對促(cu)(cu)進(jin)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長或(huo)抑制(zhi)(zhi)生(sheng)(sheng)(sheng)(sheng)長活(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)性是(shi)(shi)(shi)(shi)(shi)達到生(sheng)(sheng)(sheng)(sheng)li平衡的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要(yao)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)●提(ti)供(gong)(gong)基(ji)(ji)(ji)(ji)(ji)(ji)本(ben)(ben)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)：氨基(ji)(ji)(ji)(ji)(ji)(ji)酸(suan)、維(wei)(wei)生(sheng)(sheng)(sheng)(sheng)素(su)、無(wu)(wu)(wu)(wu)機(ji)物(wu)(wu)(wu)(wu)、脂(zhi)類物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)、核酸(suan)衍生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)等(deng)(deng)，是(shi)(shi)(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長必(bi)須(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)。●提(ti)供(gong)(gong)激(ji)(ji)(ji)素(su)和(he)(he)(he)各種(zhong)(zhong)(zhong)生(sheng)(sheng)(sheng)(sheng)長因(yin)子(zi)：胰島素(su)、腎(shen)上(shang)腺(xian)皮(pi)質(zhi)(zhi)(zhi)激(ji)(ji)(ji)素(su)（氫化(hua)可(ke)(ke)(ke)(ke)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)松、地塞(sai)(sai)米松）、類固(gu)醇(chun)激(ji)(ji)(ji)素(su)（雌二(er)醇(chun)、睪酮、孕酮）等(deng)(deng)。生(sheng)(sheng)(sheng)(sheng)長因(yin)子(zi)如(ru)(ru)(ru)成(cheng)(cheng)(cheng)纖維(wei)(wei)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)生(sheng)(sheng)(sheng)(sheng)長因(yin)子(zi)、表(biao)皮(pi)生(sheng)(sheng)(sheng)(sheng)長因(yin)子(zi)、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)小(xiao)板生(sheng)(sheng)(sheng)(sheng)長因(yin)子(zi)等(deng)(deng)。●提(ti)供(gong)(gong)結(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)：結(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)(shi)(shi)攜(xie)帶重要(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)低分(fen)(fen)(fen)子(zi)量(liang)(liang)(liang)(liang)(liang)(liang)物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)，如(ru)(ru)(ru)白(bai)(bai)(bai)(bai)(bai)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)攜(xie)帶維(wei)(wei)生(sheng)(sheng)(sheng)(sheng)素(su)、脂(zhi)肪(fang)、以(yi)(yi)(yi)(yi)(yi)及(ji)激(ji)(ji)(ji)素(su)等(deng)(deng)，轉鐵(tie)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)攜(xie)帶鐵(tie)。結(jie)合(he)(he)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)在(zai)(zai)(zai)(zai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)代(dai)謝(xie)過(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)起(qi)(qi)(qi)重要(yao)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。●提(ti)供(gong)(gong)促(cu)(cu)接(jie)(jie)(jie)觸和(he)(he)(he)伸(shen)展因(yin)子(zi)使(shi)(shi)(shi)(shi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)貼(tie)(tie)壁(bi)免(mian)受機(ji)械損(sun)(sun)傷。●對培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)起(qi)(qi)(qi)到某些(xie)(xie)保(bao)(bao)護作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)：有(you)(you)一(yi)(yi)(yi)(yi)(yi)些(xie)(xie)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)，如(ru)(ru)(ru)內(nei)皮(pi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)、骨髓樣細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)釋放蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)酶，血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)中(zhong)(zhong)(zhong)(zhong)含(han)有(you)(you)抗蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)酶成(cheng)(cheng)(cheng)分(fen)(fen)(fen)，起(qi)(qi)(qi)到中(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。這種(zhong)(zhong)(zhong)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)是(shi)(shi)(shi)(shi)(shi)偶然(ran)發現(xian)(xian)(xian)(xian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，則(ze)(ze)有(you)(you)目的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)來終止(zhi)(zhi)胰蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)酶的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消(xiao)化(hua)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。因(yin)為(wei)(wei)(wei)胰蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)酶已經(jing)(jing)被廣泛(fan)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)貼(tie)(tie)壁(bi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消(xiao)化(hua)傳代(dai)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)形成(cheng)(cheng)(cheng)了(le)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)粘(zhan)度(du)，可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)保(bao)(bao)護細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)免(mian)受機(ji)械損(sun)(sun)傷，特別(bie)(bie)是(shi)(shi)(shi)(shi)(shi)在(zai)(zai)(zai)(zai)懸浮培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)攪拌時(shi)(shi)(shi)(shi)(shi)，粘(zhan)度(du)起(qi)(qi)(qi)到重要(yao)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)還含(han)有(you)(you)一(yi)(yi)(yi)(yi)(yi)些(xie)(xie)微(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)元(yuan)素(su)和(he)(he)(he)離(li)(li)子(zi)，他們在(zai)(zai)(zai)(zai)代(dai)謝(xie)解(jie)(jie)(jie)(jie)(jie)(jie)毒(du)(du)(du)中(zhong)(zhong)(zhong)(zhong)起(qi)(qi)(qi)重要(yao)作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)，如(ru)(ru)(ru)SeO3，硒等(deng)(deng)。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)條(tiao)件(jian)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)時(shi)(shi)(shi)(shi)(shi)應(ying)(ying)(ying)(ying)根據細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)類和(he)(he)(he)目的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)等(deng)(deng)選擇(ze)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)方(fang)(fang)法(fa)、培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)，制(zhi)(zhi)定(ding)(ding)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)條(tiao)件(jian)(溫(wen)度(du)、pH值、時(shi)(shi)(shi)(shi)(shi)間，對氧的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)需(xu)(xu)求(qiu)與否(fou)等(deng)(deng))。一(yi)(yi)(yi)(yi)(yi)般(ban)操作(zuo)(zuo)(zuo)(zuo)步(bu)驟為(wei)(wei)(wei)先(xian)將(jiang)(jiang)(jiang)標(biao)本(ben)(ben)接(jie)(jie)(jie)種(zhong)(zhong)(zhong)于(yu)固(gu)體(ti)(ti)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)，做(zuo)分(fen)(fen)(fen)離(li)(li)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)。再(zai)進(jin)一(yi)(yi)(yi)(yi)(yi)步(bu)對所(suo)得單個(ge)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)落進(jin)行形態(tai)、生(sheng)(sheng)(sheng)(sheng)化(hua)及(ji)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)學(xue)(xue)反(fan)應(ying)(ying)(ying)(ying)鑒(jian)定(ding)(ding)。培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)常用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)牛(niu)(niu)(niu)肉湯、蛋(dan)(dan)(dan)(dan)白(bai)(bai)(bai)(bai)(bai)、氯化(hua)鈉、葡萄糖、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)等(deng)(deng)和(he)(he)(he)某些(xie)(xie)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)所(suo)需(xu)(xu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)特殊物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)配(pei)(pei)(pei)(pei)制(zhi)(zhi)成(cheng)(cheng)(cheng)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)、半固(gu)體(ti)(ti)、固(gu)體(ti)(ti)等(deng)(deng)。一(yi)(yi)(yi)(yi)(yi)般(ban)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)可(ke)(ke)(ke)(ke)在(zai)(zai)(zai)(zai)有(you)(you)氧條(tiao)件(jian)下，37℃中(zhong)(zhong)(zhong)(zhong)放18~24小(xiao)時(shi)(shi)(shi)(shi)(shi)生(sheng)(sheng)(sheng)(sheng)長。厭氧菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)則(ze)(ze)需(xu)(xu)在(zai)(zai)(zai)(zai)無(wu)(wu)(wu)(wu)氧環(huan)境中(zhong)(zhong)(zhong)(zhong)放2~3天后(hou)(hou)(hou)生(sheng)(sheng)(sheng)(sheng)長。個(ge)別(bie)(bie)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)如(ru)(ru)(ru)結(jie)核菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)要(yao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)1個(ge)月(yue)(yue)之久。細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)條(tiao)件(jian)由(you)于(yu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)無(wu)(wu)(wu)(wu)處(chu)(chu)不(bu)(bu)(bu)(bu)(bu)在(zai)(zai)(zai)(zai)，因(yin)此(ci)從制(zhi)(zhi)備(bei)(bei)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)時(shi)(shi)(shi)(shi)(shi)開始(shi)，整個(ge)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)過(guo)(guo)(guo)程必(bi)須(xu)(xu)按(an)(an)(an)無(wu)(wu)(wu)(wu)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)操作(zuo)(zuo)(zuo)(zuo)要(yao)求(qiu)進(jin)行，否(fou)則(ze)(ze)外界(jie)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)污(wu)(wu)染標(biao)本(ben)(ben)，會(hui)(hui)導致錯誤(wu)結(jie)果;而培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)致病(bing)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)一(yi)(yi)(yi)(yi)(yi)旦污(wu)(wu)染環(huan)境，就會(hui)(hui)引起(qi)(qi)(qi)交叉感染。以(yi)(yi)(yi)(yi)(yi)疾病(bing)診斷為(wei)(wei)(wei)目的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)進(jin)行的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)，要(yao)選擇(ze)合(he)(he)適的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)標(biao)本(ben)(ben)(血(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)(xue)、尿、便、膿液(ye)(ye)(ye)(ye)(ye)、分(fen)(fen)(fen)泌物(wu)(wu)(wu)(wu)等(deng)(deng))，并(bing)(bing)(bing)應(ying)(ying)(ying)(ying)結(jie)合(he)(he)臨床情況解(jie)(jie)(jie)(jie)(jie)(jie)釋所(suo)得結(jie)果。培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)步(bu)驟以(yi)(yi)(yi)(yi)(yi)光(guang)(guang)合(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)方(fang)(fang)法(fa)為(wei)(wei)(wei)例。光(guang)(guang)合(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)法(fa)，按(an)(an)(an)次序(xu)分(fen)(fen)(fen)為(wei)(wei)(wei)容(rong)(rong)器(qi)、工(gong)具的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消(xiao)毒(du)(du)(du)，培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)(zhi)備(bei)(bei)，接(jie)(jie)(jie)種(zhong)(zhong)(zhong)和(he)(he)(he)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)管理(li)四個(ge)步(bu)驟。(一(yi)(yi)(yi)(yi)(yi))容(rong)(rong)器(qi)、工(gong)具的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)消(xiao)毒(du)(du)(du)參考、此(ci)處(chu)(chu)從略。(二(er))培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)制(zhi)(zhi)備(bei)(bei)1.培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)水(shui)(shui)(shui)如(ru)(ru)(ru)果培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)(guang)合(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)是(shi)(shi)(shi)(shi)(shi)淡水(shui)(shui)(shui)種(zhong)(zhong)(zhong)，菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)蒸餾水(shui)(shui)(shui)，生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)消(xiao)毒(du)(du)(du)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)自來水(shui)(shui)(shui)(或(huo)井(jing)水(shui)(shui)(shui))配(pei)(pei)(pei)(pei)制(zhi)(zhi)。如(ru)(ru)(ru)果培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)光(guang)(guang)合(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)是(shi)(shi)(shi)(shi)(shi)海水(shui)(shui)(shui)種(zhong)(zhong)(zhong)，則(ze)(ze)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)天然(ran)海水(shui)(shui)(shui)配(pei)(pei)(pei)(pei)制(zhi)(zhi)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)，注意(yi)(yi)在(zai)(zai)(zai)(zai)海水(shui)(shui)(shui)中(zhong)(zhong)(zhong)(zhong)加(jia)入(ru)磷元(yuan)素(su)時(shi)(shi)(shi)(shi)(shi)，不(bu)(bu)(bu)(bu)(bu)能用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)磷酸(suan)氫二(er)鉀(jia)(jia)，應(ying)(ying)(ying)(ying)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)磷酸(suan)二(er)氫鉀(jia)(jia)，不(bu)(bu)(bu)(bu)(bu)然(ran)會(hui)(hui)產(chan)(chan)生(sheng)(sheng)(sheng)(sheng)大(da)(da)量(liang)(liang)(liang)(liang)(liang)(liang)沉(chen)(chen)淀(dian)(dian)。2.滅(mie)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)和(he)(he)(he)消(xiao)毒(du)(du)(du)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)應(ying)(ying)(ying)(ying)連同培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)容(rong)(rong)器(qi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)高壓蒸氣滅(mie)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)鍋(guo)滅(mie)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)。小(xiao)型(xing)(xing)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)性培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)可(ke)(ke)(ke)(ke)把(ba)配(pei)(pei)(pei)(pei)好的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)液(ye)(ye)(ye)(ye)(ye)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)普通鋁(lv)鍋(guo)或(huo)大(da)(da)型(xing)(xing)三(san)角燒瓶(ping)(ping)煮沸消(xiao)毒(du)(du)(du)。大(da)(da)型(xing)(xing)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)性培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)則(ze)(ze)把(ba)經(jing)(jing)沉(chen)(chen)淀(dian)(dian)砂濾(lv)后(hou)(hou)(hou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)水(shui)(shui)(shui)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)漂白(bai)(bai)(bai)(bai)(bai)粉(fen)(或(huo)漂白(bai)(bai)(bai)(bai)(bai)液(ye)(ye)(ye)(ye)(ye))消(xiao)毒(du)(du)(du)后(hou)(hou)(hou)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。3.培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)配(pei)(pei)(pei)(pei)制(zhi)(zhi)根據所(suo)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)種(zhong)(zhong)(zhong)類的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)營(ying)(ying)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)需(xu)(xu)要(yao)選擇(ze)合(he)(he)適的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)配(pei)(pei)(pei)(pei)方(fang)(fang)。按(an)(an)(an)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)配(pei)(pei)(pei)(pei)方(fang)(fang)把(ba)所(suo)需(xu)(xu)物(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)稱量(liang)(liang)(liang)(liang)(liang)(liang)，逐(zhu)一(yi)(yi)(yi)(yi)(yi)溶解(jie)(jie)(jie)(jie)(jie)(jie)，混(hun)合(he)(he)，配(pei)(pei)(pei)(pei)成(cheng)(cheng)(cheng)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)。也可(ke)(ke)(ke)(ke)先(xian)配(pei)(pei)(pei)(pei)成(cheng)(cheng)(cheng)母液(ye)(ye)(ye)(ye)(ye)，使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)時(shi)(shi)(shi)(shi)(shi)按(an)(an)(an)比(bi)例加(jia)入(ru)一(yi)(yi)(yi)(yi)(yi)定(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)量(liang)(liang)(liang)(liang)(liang)(liang)即可(ke)(ke)(ke)(ke)。(三(san))接(jie)(jie)(jie)種(zhong)(zhong)(zhong)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)基(ji)(ji)(ji)(ji)(ji)(ji)配(pei)(pei)(pei)(pei)好后(hou)(hou)(hou)，應(ying)(ying)(ying)(ying)立即進(jin)行接(jie)(jie)(jie)種(zhong)(zhong)(zhong)。光(guang)(guang)合(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)生(sheng)(sheng)(sheng)(sheng)產(chan)(chan)性培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)按(an)(an)(an)種(zhong)(zhong)(zhong)量(liang)(liang)(liang)(liang)(liang)(liang)比(bi)較高，一(yi)(yi)(yi)(yi)(yi)般(ban)為(wei)(wei)(wei)20%~50%，即菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)種(zhong)(zhong)(zhong)母液(ye)(ye)(ye)(ye)(ye)量(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)新(xin)配(pei)(pei)(pei)(pei)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)液(ye)(ye)(ye)(ye)(ye)雖(sui)之比(bi)為(wei)(wei)(wei)1∶4~1∶1，不(bu)(bu)(bu)(bu)(bu)應(ying)(ying)(ying)(ying)低于(yu)20%，尤其是(shi)(shi)(shi)(shi)(shi)微(wei)(wei)(wei)氣培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)，接(jie)(jie)(jie)種(zhong)(zhong)(zhong)量(liang)(liang)(liang)(liang)(liang)(liang)更應(ying)(ying)(ying)(ying)高些(xie)(xie)，否(fou)則(ze)(ze)光(guang)(guang)合(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)在(zai)(zai)(zai)(zai)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)液(ye)(ye)(ye)(ye)(ye)中(zhong)(zhong)(zhong)(zhong)很難占＊＊優勢，影(ying)響培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)＊終產(chan)(chan)量(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)。(四)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)管理(li)光(guang)(guang)合(he)(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)(jun)(jun)(jun)(jun)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)培(pei)(pei)(pei)(pei)(pei)(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)過(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)，管理(li)工(gong)作(zuo)(zuo)(zuo)(zuo)包括日常管理(li)操作(zuo)(zuo)(zuo)(zuo)和(he)(he)(he)測(ce)試(shi)，生(sheng)(sheng)(sheng)(sheng)長情況的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)觀察、檢(jian)查(cha)以(yi)(yi)(yi)(yi)(yi)及(ji)出(chu)(chu)現(xian)(xian)(xian)(xian)問(wen)題(ti)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)(fen)析處(chu)(chu)理(li)等(deng)(deng)三(san)個(ge)方(fang)(fang)面。
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