γ-谷氨(an)酰轉肽酶(γ-GT)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)于(yu)(yu)科研(yan)(yan)實驗(yan)(yan),不(bu)(bu)(bu)用(yong)(yong)(yong)(yong)于(yu)(yu)臨(lin)床。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)可(ke)(ke)(ke)以(yi)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)類(lei)型(xing)(xing)有(you)(you)哪些???生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實驗(yan)(yan)原理(li)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)基(ji)于(yu)(yu)化(hua)(hua)(hua)(hua)(hua)(hua)學(xue)(xue)反(fan)(fan)(fan)應(ying),理(li)論(lun)上(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)受生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)種(zhong)(zhong)屬(shu)及(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)類(lei)型(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)(xian)制(zhi)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通(tong)常(chang)只分(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)類(lei)型(xing)(xing),不(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)種(zhong)(zhong)屬(shu)。但(dan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)某些指(zhi)(zhi)(zhi)標(biao)(biao)在(zai)(zai)(zai)不(bu)(bu)(bu)同(tong)種(zhong)(zhong)屬(shu)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)類(lei)型(xing)(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方式不(bu)(bu)(bu)同(tong)。因(yin)(yin)此(ci),具(ju)(ju)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)情(qing)況請參見說(shuo)(shuo)明(ming)書(shu)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)與(yu)(yu)技(ji)術(shu)(shu)支(zhi)持聯系(xi)確認。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)般(ban)要(yao)(yao)(yao)(yao)求:1)于(yu)(yu)生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)無(wu)溶血(xue)(xue)(xue)(xue)、脂血(xue)(xue)(xue)(xue)、的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現象。組(zu)(zu)織(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)需(xu)將血(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)洗凈(jing)(jing)擦(ca)干(gan)后(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)稱重(zhong)(zhong)(zhong)處(chu)理(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)處(chu)理(li)后(hou)(hou)盡(jin)快(kuai)(kuai)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且保(bao)(bao)(bao)存(cun)(cun)于(yu)(yu)冰(bing)(bing)(bing)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)之(zhi)(zhi)上(shang)(shang)(shang)(shang),待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。求3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)需(xu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing),若(ruo)(ruo)(ruo)(ruo)不(bu)(bu)(bu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing),需(xu)離(li)心(xin)(xin)(xin)后(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特(te)(te)(te)殊指(zhi)(zhi)(zhi)標(biao)(biao)除外)。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)值(zhi)應(ying)在(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)線(xian)性(xing)(xing)(xing)范(fan)圍(wei)(wei)內(nei)(nei),若(ruo)(ruo)(ruo)(ruo)超過(guo)(guo)線(xian)性(xing)(xing)(xing)范(fan)圍(wei)(wei),需(xu)稀(xi)釋后(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若(ruo)(ruo)(ruo)(ruo)低于(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)圍(wei)(wei),需(xu)增(zeng)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)后(hou)(hou)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。微(wei)(wei)量(liang)(liang)(liang)(liang)法(fa)和(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)光(guang)光(guang)度(du)(du)(du)(du)(du)(du)法(fa)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別?分(fen)(fen)(fen)(fen)(fen)光(guang)光(guang)度(du)(du)(du)(du)(du)(du)法(fa):主流(liu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)為(wei)(wei)(wei)(wei)(wei)1mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)(yi)器(qi)(qi)(qi)分(fen)(fen)(fen)(fen)(fen)光(guang)光(guang)度(du)(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji),能(neng)(neng)(neng)(neng)(neng)(neng)夠大限(xian)(xian)(xian)度(du)(du)(du)(du)(du)(du)地滿(man)足絕(jue)大多(duo)數(shu)(shu)用(yong)(yong)(yong)(yong)戶(hu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)需(xu)要(yao)(yao)(yao)(yao)。微(wei)(wei)量(liang)(liang)(liang)(liang)法(fa):主流(liu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)為(wei)(wei)(wei)(wei)(wei)0.2mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)(yi)器(qi)(qi)(qi)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)分(fen)(fen)(fen)(fen)(fen)光(guang)光(guang)度(du)(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji),測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)更加(jia)(jia)簡便快(kuai)(kuai)捷。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)規格(ge)(ge)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)100管(guan)(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)50管(guan)(guan)(guan)(guan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)什么(me)意(yi)(yi)思?(1)50管(guan)(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):50管(guan)(guan)(guan)(guan)指(zhi)(zhi)(zhi)能(neng)(neng)(neng)(neng)(neng)(neng)夠做(zuo)(zuo)50次(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)(zhi)(zhi)可(ke)(ke)(ke)以(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)48個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)。如(ru)(ru)果(guo)每(mei)個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)重(zhong)(zhong)(zhong)復(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci)(ci),那(nei)么(me)只能(neng)(neng)(neng)(neng)(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)16個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)。其(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)2次(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)于(yu)(yu)空白(bai)管(guan)(guan)(guan)(guan)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)者(zhe)(zhe)對(dui)(dui)照(zhao)管(guan)(guan)(guan)(guan)。值(zhi)得(de)特(te)(te)(te)別注(zhu)(zhu)(zhu)意(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi),50管(guan)(guan)(guan)(guan)/24樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由于(yu)(yu)每(mei)個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)(dou)(dou)需(xu)要(yao)(yao)(yao)(yao)做(zuo)(zuo)對(dui)(dui)照(zhao),因(yin)(yin)此(ci)能(neng)(neng)(neng)(neng)(neng)(neng)夠測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)(shu)量(liang)(liang)(liang)(liang)僅為(wei)(wei)(wei)(wei)(wei)8個(ge)(ge)(假(jia)設(she)做(zuo)(zuo)3次(ci)(ci)(ci)重(zhong)(zhong)(zhong)復(fu))(2)100管(guan)(guan)(guan)(guan)/96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):100管(guan)(guan)(guan)(guan)指(zhi)(zhi)(zhi)能(neng)(neng)(neng)(neng)(neng)(neng)夠做(zuo)(zuo)100次(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)(zhi)(zhi)可(ke)(ke)(ke)以(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)96個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin),如(ru)(ru)果(guo)每(mei)個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)重(zhong)(zhong)(zhong)復(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci)(ci),那(nei)么(me)只能(neng)(neng)(neng)(neng)(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)32個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)。其(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)4次(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)于(yu)(yu)空白(bai)管(guan)(guan)(guan)(guan)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)者(zhe)(zhe)對(dui)(dui)照(zhao)管(guan)(guan)(guan)(guan)。注(zhu)(zhu)(zhu)意(yi)(yi)由于(yu)(yu)瓶(ping)璧和(he)(he)(he)(he)(he)(he)(he)移液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)器(qi)(qi)(qi)吸附等(deng)(deng)(deng)(deng)(deng)(deng),通(tong)常(chang)要(yao)(yao)(yao)(yao)比(bi)(bi)(bi)(bi)(bi)(bi)理(li)論(lun)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)次(ci)(ci)(ci)數(shu)(shu)少1~3次(ci)(ci)(ci)。其(qi)(qi)(qi)(qi)它規格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)義依(yi)次(ci)(ci)(ci)類(lei)推(tui)。值(zhi)得(de)特(te)(te)(te)別注(zhu)(zhu)(zhu)意(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi),100管(guan)(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由于(yu)(yu)每(mei)個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)(dou)(dou)需(xu)要(yao)(yao)(yao)(yao)做(zuo)(zuo)對(dui)(dui)照(zhao),因(yin)(yin)此(ci)能(neng)(neng)(neng)(neng)(neng)(neng)夠測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)(shu)量(liang)(liang)(liang)(liang)僅為(wei)(wei)(wei)(wei)(wei)16個(ge)(ge)(假(jia)設(she)做(zuo)(zuo)3次(ci)(ci)(ci)重(zhong)(zhong)(zhong)復(fu))。如(ru)(ru)何防(fang)止(zhi)(zhi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)失(shi)(shi)(shi)(shi)(shi)效?????不(bu)(bu)(bu)要(yao)(yao)(yao)(yao)過(guo)(guo)早訂購,以(yi)免試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)失(shi)(shi)(shi)(shi)(shi)效.收到(dao)(dao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)后(hou)(hou),檢(jian)(jian)(jian)(jian)(jian)(jian)查外包裝是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)正常(chang)。馬(ma)上(shang)(shang)(shang)(shang)打開,按(an)(an)照(zhao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)說(shuo)(shuo)明(ming)書(shu)核(he)對(dui)(dui)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)數(shu)(shu)量(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)正確,注(zhu)(zhu)(zhu)意(yi)(yi)瓶(ping)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)狀態(tai)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)與(yu)(yu)說(shuo)(shuo)明(ming)書(shu)一(yi)(yi)(yi)(yi)致。如(ru)(ru)果(guo)有(you)(you)問題,或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)者(zhe)(zhe)懷(huai)疑(yi),馬(ma)上(shang)(shang)(shang)(shang)聯系(xi)公(gong)(gong)(gong)司銷售,進(jin)(jin)(jin)(jin)行(xing)(xing)確認。如(ru)(ru)果(guo)沒有(you)(you)問題,請務(wu)必按(an)(an)照(zhao)說(shuo)(shuo)明(ming)書(shu)進(jin)(jin)(jin)(jin)行(xing)(xing)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)(bao)存(cun)(cun)。注(zhu)(zhu)(zhu)意(yi)(yi),不(bu)(bu)(bu)同(tong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji),可(ke)(ke)(ke)能(neng)(neng)(neng)(neng)(neng)(neng)需(xu)要(yao)(yao)(yao)(yao)分(fen)(fen)(fen)(fen)(fen)別保(bao)(bao)(bao)存(cun)(cun)在(zai)(zai)(zai)常(chang)溫(25°℃)、4℃和(he)(he)(he)(he)(he)(he)(he)-20℃,務(wu)必分(fen)(fen)(fen)(fen)(fen)別按(an)(an)照(zhao)要(yao)(yao)(yao)(yao)求保(bao)(bao)(bao)存(cun)(cun),不(bu)(bu)(bu)要(yao)(yao)(yao)(yao)整(zheng)(zheng)個(ge)(ge)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)放置(zhi)(zhi)(zhi)于(yu)(yu)冰(bing)(bing)(bing)箱(xiang)。如(ru)(ru)何進(jin)(jin)(jin)(jin)行(xing)(xing)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)?(1)務(wu)必在(zai)(zai)(zai)7個(ge)(ge)工作(zuo)(zuo)(zuo)(zuo)日內(nei)(nei)進(jin)(jin)(jin)(jin)行(xing)(xing)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),以(yi)確定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)該試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)適合(he)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin),以(yi)免造(zao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)和(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浪費(fei)。(2)選(xuan)擇(ze)2~3個(ge)(ge)預(yu)(yu)期差異(yi)比(bi)(bi)(bi)(bi)(bi)(bi)較(jiao)(jiao)大的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin),嚴(yan)格(ge)(ge)按(an)(an)照(zhao)說(shuo)(shuo)明(ming)書(shu)進(jin)(jin)(jin)(jin)行(xing)(xing)操作(zuo)(zuo)(zuo)(zuo),注(zhu)(zhu)(zhu)意(yi)(yi)儀(yi)(yi)器(qi)(qi)(qi)工作(zuo)(zuo)(zuo)(zuo)狀態(tai),操作(zuo)(zuo)(zuo)(zuo)規范(fan),控制(zhi)好(hao)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)條件(jian),尤其(qi)(qi)(qi)(qi)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)溫度(du)(du)(du)(du)(du)(du)。(3)如(ru)(ru)實與(yu)(yu)公(gong)(gong)(gong)司銷售溝通(tong)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)果(guo),公(gong)(gong)(gong)司技(ji)術(shu)(shu)人員(yuan)會確認測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)果(guo)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)正常(chang),是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)需(xu)要(yao)(yao)(yao)(yao)調(diao)(diao)(diao)整(zheng)(zheng),如(ru)(ru)何進(jin)(jin)(jin)(jin)行(xing)(xing)調(diao)(diao)(diao)整(zheng)(zheng),從(cong)(cong)(cong)而(er)(er)確保(bao)(bao)(bao)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)果(guo)可(ke)(ke)(ke)靠(kao)。比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)有(you)(you)哪些規格(ge)(ge)?(1)常(chang)量(liang)(liang)(liang)(liang)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min),如(ru)(ru)1mL和(he)(he)(he)(he)(he)(he)(he)3mL等(deng)(deng)(deng)(deng)(deng)(deng),常(chang)量(liang)(liang)(liang)(liang)法(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)都(dou)(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)選(xuan)用(yong)(yong)(yong)(yong)1mL體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi),只能(neng)(neng)(neng)(neng)(neng)(neng)用(yong)(yong)(yong)(yong)1mL比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min),不(bu)(bu)(bu)可(ke)(ke)(ke)以(yi)用(yong)(yong)(yong)(yong)更大體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min),否(fou)(fou)則試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)量(liang)(liang)(liang)(liang)不(bu)(bu)(bu)夠,無(wu)法(fa)正常(chang)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)。(2)微(wei)(wei)量(liang)(liang)(liang)(liang)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min),如(ru)(ru)0.25mL和(he)(he)(he)(he)(he)(he)(he)0.5mL等(deng)(deng)(deng)(deng)(deng)(deng),微(wei)(wei)量(liang)(liang)(liang)(liang)法(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)都(dou)(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)選(xuan)用(yong)(yong)(yong)(yong)0.25mL比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)。一(yi)(yi)(yi)(yi)般(ban)客戶(hu)都(dou)(dou)(dou)選(xuan)擇(ze)使(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)標(biao)(biao)儀(yi)(yi)(需(xu)要(yao)(yao)(yao)(yao)自(zi)(zi)備(bei)酶標(biao)(biao)板)(3)紫外波長檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)需(xu)要(yao)(yao)(yao)(yao)石(shi)英比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min),不(bu)(bu)(bu)可(ke)(ke)(ke)以(yi)用(yong)(yong)(yong)(yong)普通(tong)玻璃比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)。(4)不(bu)(bu)(bu)同(tong)規格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min),其(qi)(qi)(qi)(qi)外觀大小是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)(yi)(yi)致的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de),都(dou)(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)1cm光(guang)徑(也有(you)(you)0.5cm光(guang)徑的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min),不(bu)(bu)(bu)常(chang)見,也可(ke)(ke)(ke)以(yi)用(yong)(yong)(yong)(yong),但(dan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)時(shi)要(yao)(yao)(yao)(yao)注(zhu)(zhu)(zhu)意(yi)(yi)修改計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)公(gong)(gong)(gong)式),其(qi)(qi)(qi)(qi)內(nei)(nei)容積(ji)差異(yi)在(zai)(zai)(zai)于(yu)(yu)壁的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)厚度(du)(du)(du)(du)(du)(du)。代(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)優點(1)更加(jia)(jia)省(sheng)事兒(er),完(wan)(wan)全(quan)(quan)不(bu)(bu)(bu)必為(wei)(wei)(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)犯(fan)難。用(yong)(yong)(yong)(yong)戶(hu)只要(yao)(yao)(yao)(yao)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)供樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)和(he)(he)(he)(he)(he)(he)(he)想測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)指(zhi)(zhi)(zhi)標(biao)(biao),就不(bu)(bu)(bu)再(zai)需(xu)要(yao)(yao)(yao)(yao)為(wei)(wei)(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)操心(xin)(xin)(xin)了,不(bu)(bu)(bu)再(zai)受到(dao)(dao)操作(zuo)(zuo)(zuo)(zuo)技(ji)能(neng)(neng)(neng)(neng)(neng)(neng)、儀(yi)(yi)器(qi)(qi)(qi)設(she)備(bei)和(he)(he)(he)(he)(he)(he)(he)時(shi)間的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)(xian)制(zhi),就等(deng)(deng)(deng)(deng)(deng)(deng)著拿到(dao)(dao)數(shu)(shu)據寫論(lun)文。(2)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)數(shu)(shu)據更有(you)(you)保(bao)(bao)(bao)障(zhang)。嚴(yan)格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)管(guan)(guan)(guan)(guan)理(li)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi),從(cong)(cong)(cong)而(er)(er)保(bao)(bao)(bao)證(zheng)了測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)真實可(ke)(ke)(ke)靠(kao)。我們還建(jian)立了免費(fei)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)制(zhi)度(du)(du)(du)(du)(du)(du)。(3)防(fang)止(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)損(sun)失(shi)(shi)(shi)(shi)(shi)無(wu)論(lun)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)戶(hu)自(zi)(zi)己完(wan)(wan)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)全(quan)(quan)過(guo)(guo)程,還是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)購買試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)完(wan)(wan)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),都(dou)(dou)(dou)可(ke)(ke)(ke)能(neng)(neng)(neng)(neng)(neng)(neng)因(yin)(yin)為(wei)(wei)(wei)(wei)(wei)缺乏測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)經(jing)驗(yan)(yan),導(dao)致測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)失(shi)(shi)(shi)(shi)(shi)敗(bai),從(cong)(cong)(cong)而(er)(er)損(sun)失(shi)(shi)(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情(qing)況。對(dui)(dui)于(yu)(yu)生(sheng)(sheng)(sheng)命科學(xue)(xue)研(yan)(yan)究而(er)(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)＊珍貴的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)一(yi)(yi)(yi)(yi)旦損(sun)失(shi)(shi)(shi)(shi)(shi),輕則造(zao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)研(yan)(yan)究進(jin)(jin)(jin)(jin)度(du)(du)(du)(du)(du)(du)被(bei)耽擱,重(zhong)(zhong)(zhong)則造(zao)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)研(yan)(yan)究完(wan)(wan)全(quan)(quan)失(shi)(shi)(shi)(shi)(shi)敗(bai)!代(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)能(neng)(neng)(neng)(neng)(neng)(neng)夠充(chong)分(fen)(fen)(fen)(fen)(fen)保(bao)(bao)(bao)證(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)功(gong),從(cong)(cong)(cong)而(er)(er)防(fang)止(zhi)(zhi)了樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)損(sun)失(shi)(shi)(shi)(shi)(shi)。當然,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)功(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti),而(er)(er)且樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)只有(you)(you)用(yong)(yong)(yong)(yong)戶(hu)自(zi)(zi)己才能(neng)(neng)(neng)(neng)(neng)(neng)控制(zhi)和(he)(he)(he)(he)(he)(he)(he)保(bao)(bao)(bao)證(zheng)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)本(ben)(ben)(ben)(ben)(ben)原理(li)??生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he),又稱生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he),是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)利(li)用(yong)(yong)(yong)(yong)特(te)(te)(te)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)、動(dong)力學(xue)(xue)及(ji)(ji)化(hua)(hua)(hua)(hua)(hua)(hua)學(xue)(xue)反(fan)(fan)(fan)應(ying)等(deng)(deng)(deng)(deng)(deng)(deng)技(ji)術(shu)(shu)制(zhi)備(bei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he),用(yong)(yong)(yong)(yong)于(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)生(sheng)(sheng)(sheng)理(li)學(xue)(xue)、病理(li)學(xue)(xue)、藥理(li)學(xue)(xue)等(deng)(deng)(deng)(deng)(deng)(deng)領域中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)特(te)(te)(te)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)分(fen)(fen)(fen)(fen)(fen)子(zi)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)化(hua)(hua)(hua)(hua)(hua)(hua)學(xue)(xue)物(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)包括(kuo)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)、酶試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)、探針(zhen)等(deng)(deng)(deng)(deng)(deng)(deng)多(duo)個(ge)(ge)組(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)部(bu)分(fen)(fen)(fen)(fen)(fen),其(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)＊常(chang)用(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)-酶法(fa)。底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)-酶法(fa)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)本(ben)(ben)(ben)(ben)(ben)原理(li)。在(zai)(zai)(zai)該過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)與(yu)(yu)酶在(zai)(zai)(zai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)膜上(shang)(shang)(shang)(shang)結(jie)合(he)形成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)基(ji)質(zhi)(zhi)(zhi)酶,進(jin)(jin)(jin)(jin)而(er)(er)形成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)-酶復(fu)合(he)物(wu)(wu)(wu)(wu)(wu)(wu)。隨著時(shi)間的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)推(tui)移,底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)-酶復(fu)合(he)物(wu)(wu)(wu)(wu)(wu)(wu)逐漸分(fen)(fen)(fen)(fen)(fen)解,釋放出分(fen)(fen)(fen)(fen)(fen)子(zi)小的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)子(zi)大的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)未(wei)完(wan)(wan)全(quan)(quan)消化(hua)(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)。未(wei)完(wan)(wan)全(quan)(quan)消化(hua)(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)可(ke)(ke)(ke)以(yi)被(bei)一(yi)(yi)(yi)(yi)系(xi)列的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶代(dai)(dai)謝(xie)(xie),同(tong)時(shi)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)、酶和(he)(he)(he)(he)(he)(he)(he)代(dai)(dai)謝(xie)(xie)產(chan)物(wu)(wu)(wu)(wu)(wu)(wu)都(dou)(dou)(dou)會對(dui)(dui)復(fu)合(he)物(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)穩定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)產(chan)生(sheng)(sheng)(sheng)影響(xiang),從(cong)(cong)(cong)而(er)(er)影響(xiang)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)可(ke)(ke)(ke)以(yi)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)類(lei)型(xing)(xing)有(you)(you)哪些?? ?生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實驗(yan)(yan)原理(li)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)基(ji)于(yu)(yu)化(hua)(hua)(hua)(hua)(hua)(hua)學(xue)(xue)反(fan)(fan)(fan)應(ying),理(li)論(lun)上(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)受生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)種(zhong)(zhong)屬(shu)及(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)類(lei)型(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)(xian)制(zhi)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通(tong)常(chang)只分(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)類(lei)型(xing)(xing),不(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)種(zhong)(zhong)屬(shu)。但(dan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)某些指(zhi)(zhi)(zhi)標(biao)(biao)在(zai)(zai)(zai)不(bu)(bu)(bu)同(tong)種(zhong)(zhong)屬(shu)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)類(lei)型(xing)(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方式不(bu)(bu)(bu)同(tong)。因(yin)(yin)此(ci),具(ju)(ju)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)情(qing)況請參見說(shuo)(shuo)明(ming)書(shu)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)與(yu)(yu)技(ji)術(shu)(shu)支(zhi)持聯系(xi)確認。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)般(ban)要(yao)(yao)(yao)(yao)求:1)于(yu)(yu)生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)＊-好(hao)無(wu)溶血(xue)(xue)(xue)(xue)、脂血(xue)(xue)(xue)(xue)、黃-疸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現象。組(zu)(zu)織(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)需(xu)將血(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)洗凈(jing)(jing)擦(ca)干(gan)后(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)稱重(zhong)(zhong)(zhong)處(chu)理(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)處(chu)理(li)后(hou)(hou)＊-好(hao)盡(jin)快(kuai)(kuai)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且保(bao)(bao)(bao)存(cun)(cun)于(yu)(yu)冰(bing)(bing)(bing)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)之(zhi)(zhi)上(shang)(shang)(shang)(shang),待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)需(xu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing),若(ruo)(ruo)(ruo)(ruo)不(bu)(bu)(bu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing),需(xu)離(li)心(xin)(xin)(xin)后(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特(te)(te)(te)殊指(zhi)(zhi)(zhi)標(biao)(biao)除外)。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)值(zhi)應(ying)在(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)線(xian)性(xing)(xing)(xing)范(fan)圍(wei)(wei)內(nei)(nei),若(ruo)(ruo)(ruo)(ruo)超過(guo)(guo)線(xian)性(xing)(xing)(xing)范(fan)圍(wei)(wei),需(xu)稀(xi)釋后(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若(ruo)(ruo)(ruo)(ruo)低于(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)圍(wei)(wei),需(xu)增(zeng)加(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)后(hou)(hou)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。γ-谷氨(an)酰轉肽酶(γ-GT)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)于(yu)(yu)科研(yan)(yan)實驗(yan)(yan),不(bu)(bu)(bu)用(yong)(yong)(yong)(yong)于(yu)(yu)臨(lin)床。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)意(yi)(yi)義:輔(fu)酶NAD(H)廣泛存(cun)(cun)在(zai)(zai)(zai)于(yu)(yu)動(dong)物(wu)(wu)(wu)(wu)(wu)(wu)、植物(wu)(wu)(wu)(wu)(wu)(wu)、微(wei)(wei)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)培(pei)養細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),NAD+是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)糖(tang)酵(jiao)解(EMP)和(he)(he)(he)(he)(he)(he)(he)三(san)(san)羧酸(suan)(suan)(suan)(suan)(suan)循環(huan)(TCA)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)(yao)(yao)(yao)氫受體(ti)(ti)(ti)(ti)(ti)(ti)(ti),生(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?NADH?經(jing)呼吸電(dian)(dian)子(zi)鏈(ETC)傳(chuan)遞(di)把電(dian)(dian)子(zi)交給氧,在(zai)(zai)(zai)合(he)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)?ATP?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi),形成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)大量(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?ROS,同(tong)時(shi)?NADH?再(zai)生(sheng)(sheng)(sheng)為(wei)(wei)(wei)(wei)(wei)?NAD+。糖(tang)、脂、蛋白(bai)質(zhi)(zhi)(zhi)三(san)(san)大代(dai)(dai)謝(xie)(xie)物(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)解中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)氧化(hua)(hua)(hua)(hua)(hua)(hua)反(fan)(fan)(fan)應(ying)絕(jue)大部(bu)分(fen)(fen)(fen)(fen)(fen)通(tong)過(guo)(guo)這一(yi)(yi)(yi)(yi)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)完(wan)(wan)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)。NAD(H)含(han)量(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)(he)(he)?NADH/NAD+比(bi)(bi)(bi)(bi)(bi)(bi)值(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)高(gao)(gao)低可(ke)(ke)(ke)用(yong)(yong)(yong)(yong)于(yu)(yu)評價糖(tang)酵(jiao)解和(he)(he)(he)(he)(he)(he)(he)?TCA?循環(huan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)強弱。較(jiao)(jiao)高(gao)(gao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)NAD(H)及(ji)(ji)?NADH/NAD+比(bi)(bi)(bi)(bi)(bi)(bi)值(zhi)說(shuo)(shuo)明(ming)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)呼吸耗氧量(liang)(liang)(liang)(liang)較(jiao)(jiao)高(gao)(gao),處(chu)于(yu)(yu)過(guo)(guo)氧化(hua)(hua)(hua)(hua)(hua)(hua)狀態(tai)。此(ci)外,NADH/NAD+比(bi)(bi)(bi)(bi)(bi)(bi)值(zhi)升高(gao)(gao)也可(ke)(ke)(ke)抑制(zhi)糖(tang)酵(jiao)解和(he)(he)(he)(he)(he)(he)(he)?TCA?循環(huan)。另(ling)外,NAD+降解產(chan)物(wu)(wu)(wu)(wu)(wu)(wu)對(dui)(dui)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)信號傳(chuan)導(dao)、代(dai)(dai)謝(xie)(xie)和(he)(he)(he)(he)(he)(he)(he)基(ji)因(yin)(yin)表(biao)達等(deng)(deng)(deng)(deng)(deng)(deng)具(ju)(ju)有(you)(you)重(zhong)(zhong)(zhong)要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)調(diao)(diao)(diao)控作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)原理(li):分(fen)(fen)(fen)(fen)(fen)別用(yong)(yong)(yong)(yong)酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)和(he)(he)(he)(he)(he)(he)(he)堿(jian)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH,NADH?通(tong)過(guo)(guo)?PMS?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)遞(di)氫作(zuo)(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong),還原氧化(hua)(hua)(hua)(hua)(hua)(hua)型(xing)(xing)噻(sai)唑(zuo)藍(lan)(MTT)為(wei)(wei)(wei)(wei)(wei)甲瓚,在(zai)(zai)(zai)?570nm?下(xia)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)吸光(guang)值(zhi);而(er)(er)?NAD+可(ke)(ke)(ke)被(bei)乙醇脫氫酶還原為(wei)(wei)(wei)(wei)(wei)?NADH,進(jin)(jin)(jin)(jin)一(yi)(yi)(yi)(yi)步(bu)采用(yong)(yong)(yong)(yong)?MTT?還原法(fa)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。需(xu)自(zi)(zi)備(bei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)儀(yi)(yi)器(qi)(qi)(qi)和(he)(he)(he)(he)(he)(he)(he)用(yong)(yong)(yong)(yong)品(pin)(pin)(pin)(pin)(pin):酶標(biao)(biao)儀(yi)(yi)、臺式離(li)心(xin)(xin)(xin)機、移液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)器(qi)(qi)(qi)、96?孔板、研(yan)(yan)缽(bo)、冰(bing)(bing)(bing)和(he)(he)(he)(he)(he)(he)(he)蒸(zheng)餾水(shui)(shui)(shui)(shui)(shui)。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)和(he)(he)(he)(he)(he)(he)(he)配制(zhi):酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping),4℃保(bao)(bao)(bao)存(cun)(cun);堿(jian)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping),4℃保(bao)(bao)(bao)存(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?10 mL×1?瓶(ping),4℃保(bao)(bao)(bao)存(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)二:液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?3 mL×1?瓶(ping),4℃保(bao)(bao)(bao)存(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)三(san)(san):粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping),-20?℃保(bao)(bao)(bao)存(cun)(cun),用(yong)(yong)(yong)(yong)時(shi)加(jia)(jia)入(ru)(ru)?3mL?蒸(zheng)餾水(shui)(shui)(shui)(shui)(shui),混(hun)(hun)勻(yun)(yun),用(yong)(yong)(yong)(yong)不(bu)(bu)(bu)完(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)存(cun)(cun)一(yi)(yi)(yi)(yi)周(zhou)(zhou);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)四(si):粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping),4?℃保(bao)(bao)(bao)存(cun)(cun),用(yong)(yong)(yong)(yong)時(shi)加(jia)(jia)入(ru)(ru)?3mL?蒸(zheng)餾水(shui)(shui)(shui)(shui)(shui),混(hun)(hun)勻(yun)(yun),用(yong)(yong)(yong)(yong)不(bu)(bu)(bu)完(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)存(cun)(cun)一(yi)(yi)(yi)(yi)周(zhou)(zhou);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)五(wu):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?3.6mL×1?瓶(ping),4?℃保(bao)(bao)(bao)存(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六:液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?30mL×1?瓶(ping),4?℃保(bao)(bao)(bao)存(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)七(qi):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping),4?℃保(bao)(bao)(bao)存(cun)(cun)。NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):1?血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)照(zhao)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL):酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(li)(建(jian)議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)?0.1mL?血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)),加(jia)(jia)入(ru)(ru)?1mL?酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)(shui)(shui)(shui)浴(yu)?5min(蓋緊(jin),以(yi)防(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)卻(que)后(hou)(hou),10000g 4?℃離(li)心(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)入(ru)(ru)?500μL?堿(jian)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)(shi)(shi)之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)心(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)冰(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)照(zhao)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL):堿(jian)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(li)(建(jian)議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)?0.1mL血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)),加(jia)(jia)入(ru)(ru)?1mL?堿(jian)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)(shui)(shui)(shui)浴(yu)?5min(蓋緊(jin),以(yi)防(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)卻(que)后(hou)(hou),10000g 4?℃離(li)心(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)入(ru)(ru)?500μL?酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)(shi)(shi)之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)心(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)冰(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。2?組(zu)(zu)織(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)照(zhao)組(zu)(zu)織(zhi)(zhi)(zhi)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(g):酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(li)(建(jian)議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)?0.1g?組(zu)(zu)織(zhi)(zhi)(zhi),加(jia)(jia)入(ru)(ru)?1mL酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)浴(yu)研(yan)(yan)磨(mo),95℃水(shui)(shui)(shui)(shui)(shui)浴(yu)?5min(蓋緊(jin),以(yi)防(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)卻(que)后(hou)(hou),10000g 4?℃離(li)心(xin)(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)入(ru)(ru)?500μL?堿(jian)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)(shi)(shi)之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)心(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)冰(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)照(zhao)組(zu)(zu)織(zhi)(zhi)(zhi)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(g):堿(jian)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(li)(建(jian)議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)?0.1g?組(zu)(zu)織(zhi)(zhi)(zhi),加(jia)(jia)入(ru)(ru)?1mL堿(jian)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)浴(yu)研(yan)(yan)磨(mo),95℃水(shui)(shui)(shui)(shui)(shui)浴(yu)?5min(蓋緊(jin),以(yi)防(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)卻(que)后(hou)(hou),10000g 4?℃離(li)心(xin)(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)入(ru)(ru)?500μL?酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)(shi)(shi)之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)心(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)冰(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3?細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先(xian)收集細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)到(dao)(dao)離(li)心(xin)(xin)(xin)管(guan)(guan)(guan)(guan)內(nei)(nei),棄(qi)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),按(an)(an)照(zhao)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)數(shu)(shu)量(liang)(liang)(liang)(liang)(104?個(ge)(ge)):酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(li)(建(jian)議(yi)(yi)?500?萬細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)加(jia)(jia)入(ru)(ru)?1mL?酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),超聲(sheng)波破(po)(po)碎(sui)(冰(bing)(bing)(bing)浴(yu),功(gong)率20%或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)?200W,超聲(sheng)?3s,間隔?10s,重(zhong)(zhong)(zhong)復(fu)?30?次(ci)(ci)(ci)),95℃水(shui)(shui)(shui)(shui)(shui)浴(yu)?5min(蓋緊(jin),以(yi)防(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)卻(que)后(hou)(hou),10000g 4?℃離(li)心(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)入(ru)(ru)?500μL?堿(jian)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)(shi)(shi)之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)心(xin)(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)冰(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先(xian)收集細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)到(dao)(dao)離(li)心(xin)(xin)(xin)管(guan)(guan)(guan)(guan)內(nei)(nei),棄(qi)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),按(an)(an)照(zhao)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)數(shu)(shu)量(liang)(liang)(liang)(liang)(104?個(ge)(ge)):堿(jian)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(li)(建(jian)議(yi)(yi)?500?萬細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)加(jia)(jia)入(ru)(ru)?1mL?堿(jian)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),超聲(sheng)波破(po)(po)碎(sui)(冰(bing)(bing)(bing)浴(yu),功(gong)率20%或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)?200W,超聲(sheng)?3s,間隔?10s,重(zhong)(zhong)(zhong)復(fu)?30?次(ci)(ci)(ci)),95℃水(shui)(shui)(shui)(shui)(shui)浴(yu)?5min(蓋緊(jin),以(yi)防(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)卻(que)后(hou)(hou),10000g 4?℃離(li)心(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)入(ru)(ru)?500μL?酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)(shi)(shi)之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)心(xin)(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)冰(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。充(chong)分(fen)(fen)(fen)(fen)(fen)混(hun)(hun)勻(yun)(yun),靜置(zhi)(zhi)(zhi)?5min?后(hou)(hou),20000g,25℃離(li)心(xin)(xin)(xin)?5min,棄(qi)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),沉淀中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)入(ru)(ru):混(hun)(hun)勻(yun)(yun),取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?200μL?轉移至微(wei)(wei)量(liang)(liang)(liang)(liang)石(shi)英比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)?96?孔板中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),570nm?下(xia)讀(du)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)對(dui)(dui)照(zhao)吸光(guang)值(zhi)?A1?和(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)吸光(guang)值(zhi)?A2,計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)△A=A2-A1。注(zhu)(zhu)(zhu)意(yi)(yi)事項:1、如(ru)(ru)果(guo)一(yi)(yi)(yi)(yi)次(ci)(ci)(ci)性(xing)(xing)(xing)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)數(shu)(shu)較(jiao)(jiao)多(duo),可(ke)(ke)(ke)將試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)、二、三(san)(san)和(he)(he)(he)(he)(he)(he)(he)四(si)按(an)(an)比(bi)(bi)(bi)(bi)(bi)(bi)例(li)配成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)混(hun)(hun)合(he)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。2、對(dui)(dui)照(zhao)管(guan)(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)步(bu)驟的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別:對(dui)(dui)照(zhao)管(guan)(guan)(guan)(guan)加(jia)(jia)完(wan)(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)、二、三(san)(san)、四(si)和(he)(he)(he)(he)(he)(he)(he)五(wu)后(hou)(hou)必須馬(ma)上(shang)(shang)(shang)(shang)加(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六;測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)加(jia)(jia)完(wan)(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)、二、三(san)(san)、四(si)和(he)(he)(he)(he)(he)(he)(he)五(wu)后(hou)(hou)必須反(fan)(fan)(fan)應(ying)?20min?后(hou)(hou)再(zai)加(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六。3、反(fan)(fan)(fan)應(ying)過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)注(zhu)(zhu)(zhu)意(yi)(yi)避光(guang)。4、若(ruo)(ruo)(ruo)(ruo)?NAD+測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0302,NADH?測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0222,說(shuo)(shuo)明(ming)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)輔(fu)酶含(han)量(liang)(liang)(liang)(liang)較(jiao)(jiao)低,已低于(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian)(xian)(xian),可(ke)(ke)(ke)做(zuo)(zuo)如(ru)(ru)下(xia)調(diao)(diao)(diao)整(zheng)(zheng):(1)將測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)避光(guang)靜置(zhi)(zhi)(zhi)時(shi)間?20min?延長到(dao)(dao)?60min;(2)在(zai)(zai)(zai)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)階段增(zeng)加(jia)(jia)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)(liang)(liang),即取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?0.2g?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)?0.2mL?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)加(jia)(jia)入(ru)(ru)?1mL?提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。5、由于(yu)(yu)每(mei)一(yi)(yi)(yi)(yi)個(ge)(ge)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)需(xu)要(yao)(yao)(yao)(yao)設(she)一(yi)(yi)(yi)(yi)個(ge)(ge)對(dui)(dui)照(zhao)管(guan)(guan)(guan)(guan),本(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)?100?管(guan)(guan)(guan)(guan)保(bao)(bao)(bao)證(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)?48?個(ge)(ge)?NAD+或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)?NADH.NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH?含(han)量(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan):(一(yi)(yi)(yi)(yi)) NAD+含(han)量(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)標(biao)(biao)準(zhun)條件(jian)下(xia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回(hui)歸(gui)曲線(xian)為(wei)(wei)(wei)(wei)(wei)?y = 0.1475x + 0.0302,R2 = 0.9978;其(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)(wei)(wei)?NAD+濃(nong)(nong)度(du)(du)(du)(du)(du)(du)?nmol/mL1、血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)量(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)NAD+含(han)量(liang)(liang)(liang)(liang)(nmol/mL)=[(△A -0.0302)÷0.1475×V1) ]÷(V3×V1÷V2)=135.6×(△A -0.0302)2、組(zu)(zu)織(zhi)(zhi)(zhi)、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)量(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(1)按(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)蛋白(bai)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)NAD+(nmol/mg prot)=[(△A -0.0302)÷0.1475×V1)]÷(V1×Cpr)= 6.8×(△A -0.0302)÷Cpr(2)按(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)鮮重(zhong)(zhong)(zhong)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)NAD+(nmol/g?鮮重(zhong)(zhong)(zhong))= [(△A -0.0302)÷0.1475×V1)]÷(W×V1÷V2)= 13.6×(△A -0.0302)÷W(3)按(an)(an)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)密度(du)(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)NAD+(nmol/104 cell)=[(△A -0.0302)÷0.1475×V1)]÷(500×V1÷V2)=0.027×(△A -0.0302)(二)NADH?含(han)量(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)標(biao)(biao)準(zhun)條件(jian)下(xia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回(hui)歸(gui)曲線(xian)為(wei)(wei)(wei)(wei)(wei)?y = 0.1404x + 0.0222,R2 = 0.9976;其(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)(wei)(wei)?NADH?濃(nong)(nong)度(du)(du)(du)(du)(du)(du)?nmol/mL1、血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)量(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)NADH?含(han)量(liang)(liang)(liang)(liang)(nmol/mL)= [ (△A -0.0222)÷0.1404×V1) ]÷(V3×V1÷V2)= 142.5×(△A -0.0222)2、組(zu)(zu)織(zhi)(zhi)(zhi)、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)量(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(1)按(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)蛋白(bai)濃(nong)(nong)度(du)(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)NADH (nmol/mg prot)= [ (△A -0.0222)÷0.1404×V1) ]÷(V1×Cpr)= 7.1×(△A -0.0222)÷Cpr(2)按(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)鮮重(zhong)(zhong)(zhong)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)NADH (nmol/g?鮮重(zhong)(zhong)(zhong))=[(△A -0.0222)÷0.1404×V1) ]÷(W×V1÷V2)= 14.2×(△A -0.0222)÷W(3)按(an)(an)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)密度(du)(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)NADH (nmol/104 cell)= [ (△A -0.0222)÷0.1404×V1) ]÷(500×V1÷V2)=0.028×(△A -0.0222)V1:加(jia)(jia)入(ru)(ru)反(fan)(fan)(fan)應(ying)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji),0.02mL;V2:加(jia)(jia)入(ru)(ru)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji),2mL;V3:加(jia)(jia)入(ru)(ru)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji):0.1mL;Cpr:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)蛋白(bai)質(zhi)(zhi)(zhi)濃(nong)(nong)度(du)(du)(du)(du)(du)(du),mg/mL; W:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang),g;500:細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)總數(shu)(shu),500?萬。注(zhu)(zhu)(zhu)?意(yi)(yi):?檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian)(xian)(xian)為(wei)(wei)(wei)(wei)(wei)?0.1nmol/mL?或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)?0.1nmol/g?鮮重(zhong)(zhong)(zhong)?或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)?0.001nmol/mg prot
谷胱甘肽還原(yuan)(yuan)酶(mei)(mei)(mei)(mei)(mei)（GR）測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)本(ben)(ben)(ben)(ben)品(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科研(yan)(yan)實(shi)(shi)驗(yan),不(bu)(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)臨床。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)可(ke)(ke)(ke)(ke)以(yi)(yi)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)類(lei)型有(you)(you)(you)(you)(you)哪(na)些(xie)(xie)???生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)驗(yan)原(yuan)(yuan)理(li)(li)(li)(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)基(ji)(ji)于(yu)(yu)(yu)(yu)(yu)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)反(fan)(fan)應(ying)(ying),理(li)(li)(li)(li)(li)論(lun)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)(bu)受生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)種(zhong)(zhong)屬及樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)類(lei)型的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)(xian)制(zhi)(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通常(chang)(chang)(chang)只分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)類(lei)型,不(bu)(bu)(bu)(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)種(zhong)(zhong)屬。但(dan)是(shi)(shi)(shi)(shi)(shi)(shi)某(mou)(mou)些(xie)(xie)指(zhi)(zhi)(zhi)(zhi)標在(zai)(zai)(zai)(zai)不(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)種(zhong)(zhong)屬或(huo)(huo)(huo)(huo)(huo)(huo)類(lei)型樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方式不(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)。因(yin)(yin)(yin)此(ci)(ci)(ci),具(ju)體(ti)(ti)(ti)(ti)(ti)情況(kuang)請(qing)參見(jian)(jian)說(shuo)明(ming)(ming)書(shu)(shu)(shu)或(huo)(huo)(huo)(huo)(huo)(huo)與技(ji)(ji)術(shu)支(zhi)持聯(lian)系(xi)確(que)(que)認(ren)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)般要(yao)(yao)(yao)求:1)于(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)無溶血(xue)(xue)、脂(zhi)(zhi)血(xue)(xue)、的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現(xian)象(xiang)。組(zu)(zu)(zu)織(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)(xu)將(jiang)血(xue)(xue)液(ye)(ye)(ye)(ye)(ye)洗(xi)凈擦干后(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)稱(cheng)重(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)處理(li)(li)(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)處理(li)(li)(li)(li)(li)后(hou)(hou)(hou)盡快(kuai)進(jin)(jin)(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且保存(cun)(cun)(cun)于(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)盒(he)(he)(he)之(zhi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang),待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。求3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)(xu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),若(ruo)不(bu)(bu)(bu)(bu)(bu)(bu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),需(xu)(xu)(xu)(xu)(xu)離(li)(li)心(xin)(xin)后(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特殊指(zhi)(zhi)(zhi)(zhi)標除(chu)外(wai))。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)值(zhi)應(ying)(ying)在(zai)(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)線(xian)(xian)(xian)性(xing)(xing)(xing)(xing)(xing)范(fan)圍(wei)(wei)內,若(ruo)超(chao)過(guo)(guo)(guo)線(xian)(xian)(xian)性(xing)(xing)(xing)(xing)(xing)范(fan)圍(wei)(wei),需(xu)(xu)(xu)(xu)(xu)稀釋(shi)(shi)后(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若(ruo)低(di)于(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)圍(wei)(wei),需(xu)(xu)(xu)(xu)(xu)增加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)濃(nong)度(du)(du)(du)(du)(du)后(hou)(hou)(hou)進(jin)(jin)(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。微(wei)(wei)量(liang)(liang)(liang)(liang)法(fa)和(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)光(guang)度(du)(du)(du)(du)(du)法(fa)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie)?分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)光(guang)度(du)(du)(du)(du)(du)法(fa):主流(liu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)系(xi)為(wei)(wei)(wei)1mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)器(qi)(qi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)光(guang)度(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji),能(neng)(neng)(neng)(neng)夠大(da)限(xian)(xian)(xian)度(du)(du)(du)(du)(du)地滿足絕大(da)多數(shu)(shu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)。微(wei)(wei)量(liang)(liang)(liang)(liang)法(fa):主流(liu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)系(xi)為(wei)(wei)(wei)0.2mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)器(qi)(qi)或(huo)(huo)(huo)(huo)(huo)(huo)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)光(guang)度(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji),測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)更加(jia)(jia)(jia)(jia)(jia)(jia)(jia)簡便快(kuai)捷(jie)。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)規(gui)格(ge)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)是(shi)(shi)(shi)(shi)(shi)(shi)100管(guan)(guan)(guan)(guan)(guan)(guan)和(he)(he)(he)(he)(he)50管(guan)(guan)(guan)(guan)(guan)(guan)是(shi)(shi)(shi)(shi)(shi)(shi)什么意(yi)(yi)(yi)思?(1)50管(guan)(guan)(guan)(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):50管(guan)(guan)(guan)(guan)(guan)(guan)指(zhi)(zhi)(zhi)(zhi)能(neng)(neng)(neng)(neng)夠做50次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)(zhi)(zhi)(zhi)可(ke)(ke)(ke)(ke)以(yi)(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)48個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)。如果(guo)(guo)(guo)每(mei)個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)重(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)復(fu)(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci),那(nei)么只能(neng)(neng)(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)16個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)。其中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)(you)(you)2次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)空(kong)白(bai)(bai)管(guan)(guan)(guan)(guan)(guan)(guan)或(huo)(huo)(huo)(huo)(huo)(huo)者對(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)。值(zhi)得(de)特別(bie)注(zhu)(zhu)(zhu)(zhu)意(yi)(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi),50管(guan)(guan)(guan)(guan)(guan)(guan)/24樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由(you)于(yu)(yu)(yu)(yu)(yu)每(mei)個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)做對(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao),因(yin)(yin)(yin)此(ci)(ci)(ci)能(neng)(neng)(neng)(neng)夠測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)(shu)量(liang)(liang)(liang)(liang)僅為(wei)(wei)(wei)8個(ge)(ge)(假設做3次(ci)(ci)重(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)復(fu)(fu))(2)100管(guan)(guan)(guan)(guan)(guan)(guan)/96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):100管(guan)(guan)(guan)(guan)(guan)(guan)指(zhi)(zhi)(zhi)(zhi)能(neng)(neng)(neng)(neng)夠做100次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)(zhi)(zhi)(zhi)可(ke)(ke)(ke)(ke)以(yi)(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)96個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin),如果(guo)(guo)(guo)每(mei)個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)重(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)復(fu)(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci),那(nei)么只能(neng)(neng)(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)32個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)。其中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)(you)(you)4次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)空(kong)白(bai)(bai)管(guan)(guan)(guan)(guan)(guan)(guan)或(huo)(huo)(huo)(huo)(huo)(huo)者對(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)。注(zhu)(zhu)(zhu)(zhu)意(yi)(yi)(yi)由(you)于(yu)(yu)(yu)(yu)(yu)瓶璧和(he)(he)(he)(he)(he)移(yi)液(ye)(ye)(ye)(ye)(ye)器(qi)(qi)吸附等(deng)(deng),通常(chang)(chang)(chang)要(yao)(yao)(yao)比(bi)理(li)(li)(li)(li)(li)論(lun)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)次(ci)(ci)數(shu)(shu)少1~3次(ci)(ci)。其它規(gui)格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)(han)(han)義依次(ci)(ci)類(lei)推(tui)(tui)。值(zhi)得(de)特別(bie)注(zhu)(zhu)(zhu)(zhu)意(yi)(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi),100管(guan)(guan)(guan)(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由(you)于(yu)(yu)(yu)(yu)(yu)每(mei)個(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)做對(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao),因(yin)(yin)(yin)此(ci)(ci)(ci)能(neng)(neng)(neng)(neng)夠測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)(shu)量(liang)(liang)(liang)(liang)僅為(wei)(wei)(wei)16個(ge)(ge)(假設做3次(ci)(ci)重(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)復(fu)(fu))。如何防止(zhi)(zhi)(zhi)(zhi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)失(shi)效?????不(bu)(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)過(guo)(guo)(guo)早(zao)訂購,以(yi)(yi)免(mian)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)失(shi)效.收(shou)到(dao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)后(hou)(hou)(hou),檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)查外(wai)包裝是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)正常(chang)(chang)(chang)。馬上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)打開(kai),按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)說(shuo)明(ming)(ming)書(shu)(shu)(shu)核(he)對(dui)(dui)(dui)(dui)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)數(shu)(shu)量(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)正確(que)(que),注(zhu)(zhu)(zhu)(zhu)意(yi)(yi)(yi)瓶中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)狀態(tai)(tai)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)與說(shuo)明(ming)(ming)書(shu)(shu)(shu)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)致(zhi)。如果(guo)(guo)(guo)有(you)(you)(you)(you)(you)問(wen)題(ti)(ti),或(huo)(huo)(huo)(huo)(huo)(huo)者懷(huai)疑,馬上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)聯(lian)系(xi)公司(si)銷售,進(jin)(jin)(jin)(jin)行(xing)確(que)(que)認(ren)。如果(guo)(guo)(guo)沒有(you)(you)(you)(you)(you)問(wen)題(ti)(ti),請(qing)務(wu)必(bi)(bi)按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)說(shuo)明(ming)(ming)書(shu)(shu)(shu)進(jin)(jin)(jin)(jin)行(xing)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保存(cun)(cun)(cun)。注(zhu)(zhu)(zhu)(zhu)意(yi)(yi)(yi),不(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji),可(ke)(ke)(ke)(ke)能(neng)(neng)(neng)(neng)需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)保存(cun)(cun)(cun)在(zai)(zai)(zai)(zai)常(chang)(chang)(chang)溫(25°℃)、4℃和(he)(he)(he)(he)(he)-20℃,務(wu)必(bi)(bi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)要(yao)(yao)(yao)求保存(cun)(cun)(cun),不(bu)(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)整(zheng)(zheng)個(ge)(ge)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)放(fang)置(zhi)(zhi)(zhi)于(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)箱。如何進(jin)(jin)(jin)(jin)行(xing)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)?(1)務(wu)必(bi)(bi)在(zai)(zai)(zai)(zai)7個(ge)(ge)工作(zuo)日內進(jin)(jin)(jin)(jin)行(xing)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),以(yi)(yi)確(que)(que)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)該(gai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)適合(he)(he)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin),以(yi)(yi)免(mian)造成(cheng)(cheng)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)和(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浪(lang)費。(2)選(xuan)(xuan)擇2~3個(ge)(ge)預(yu)(yu)期差異比(bi)較大(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin),嚴格(ge)按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)說(shuo)明(ming)(ming)書(shu)(shu)(shu)進(jin)(jin)(jin)(jin)行(xing)操(cao)作(zuo),注(zhu)(zhu)(zhu)(zhu)意(yi)(yi)(yi)儀(yi)器(qi)(qi)工作(zuo)狀態(tai)(tai),操(cao)作(zuo)規(gui)范(fan),控(kong)制(zhi)(zhi)(zhi)(zhi)好(hao)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)條(tiao)件(jian),尤其是(shi)(shi)(shi)(shi)(shi)(shi)溫度(du)(du)(du)(du)(du)。(3)如實(shi)(shi)與公司(si)銷售溝(gou)通預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)果(guo)(guo)(guo),公司(si)技(ji)(ji)術(shu)人員會確(que)(que)認(ren)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)果(guo)(guo)(guo)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)正常(chang)(chang)(chang),是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)調(diao)(diao)整(zheng)(zheng),如何進(jin)(jin)(jin)(jin)行(xing)調(diao)(diao)整(zheng)(zheng),從(cong)(cong)而(er)(er)確(que)(que)保您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)果(guo)(guo)(guo)可(ke)(ke)(ke)(ke)靠(kao)。比(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min)有(you)(you)(you)(you)(you)哪(na)些(xie)(xie)規(gui)格(ge)?(1)常(chang)(chang)(chang)量(liang)(liang)(liang)(liang)比(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min),如1mL和(he)(he)(he)(he)(he)3mL等(deng)(deng),常(chang)(chang)(chang)量(liang)(liang)(liang)(liang)法(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)都(dou)是(shi)(shi)(shi)(shi)(shi)(shi)選(xuan)(xuan)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)1mL體(ti)(ti)(ti)(ti)(ti)系(xi),只能(neng)(neng)(neng)(neng)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)1mL比(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min),不(bu)(bu)(bu)(bu)(bu)(bu)可(ke)(ke)(ke)(ke)以(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)更大(da)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min),否(fou)(fou)則試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)量(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)(bu)(bu)夠,無法(fa)正常(chang)(chang)(chang)比(bi)色(se)(se)(se)(se)(se)。(2)微(wei)(wei)量(liang)(liang)(liang)(liang)比(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min),如0.25mL和(he)(he)(he)(he)(he)0.5mL等(deng)(deng),微(wei)(wei)量(liang)(liang)(liang)(liang)法(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)都(dou)是(shi)(shi)(shi)(shi)(shi)(shi)選(xuan)(xuan)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)0.25mL比(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min)。一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)般客戶都(dou)選(xuan)(xuan)擇使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標儀(yi)(需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)自(zi)備(bei)(bei)酶(mei)(mei)(mei)(mei)(mei)標板(ban)(ban))(3)紫外(wai)波長(chang)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)石英(ying)比(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min),不(bu)(bu)(bu)(bu)(bu)(bu)可(ke)(ke)(ke)(ke)以(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)普通玻璃比(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min)。(4)不(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)規(gui)格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min),其外(wai)觀大(da)小是(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)致(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de),都(dou)是(shi)(shi)(shi)(shi)(shi)(shi)1cm光(guang)徑(jing)(也(ye)有(you)(you)(you)(you)(you)0.5cm光(guang)徑(jing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min),不(bu)(bu)(bu)(bu)(bu)(bu)常(chang)(chang)(chang)見(jian)(jian),也(ye)可(ke)(ke)(ke)(ke)以(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong),但(dan)是(shi)(shi)(shi)(shi)(shi)(shi)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)時(shi)要(yao)(yao)(yao)注(zhu)(zhu)(zhu)(zhu)意(yi)(yi)(yi)修(xiu)改計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)公式),其內容(rong)積(ji)(ji)(ji)差異在(zai)(zai)(zai)(zai)于(yu)(yu)(yu)(yu)(yu)壁的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)厚(hou)度(du)(du)(du)(du)(du)。代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)優(you)點(1)更加(jia)(jia)(jia)(jia)(jia)(jia)(jia)省(sheng)事兒,完(wan)(wan)(wan)全(quan)(quan)不(bu)(bu)(bu)(bu)(bu)(bu)必(bi)(bi)為(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)犯難。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶只要(yao)(yao)(yao)提(ti)(ti)(ti)(ti)(ti)(ti)供樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)和(he)(he)(he)(he)(he)想測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)指(zhi)(zhi)(zhi)(zhi)標,就不(bu)(bu)(bu)(bu)(bu)(bu)再(zai)需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)為(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)操(cao)心(xin)(xin)了,不(bu)(bu)(bu)(bu)(bu)(bu)再(zai)受到(dao)操(cao)作(zuo)技(ji)(ji)能(neng)(neng)(neng)(neng)、儀(yi)器(qi)(qi)設備(bei)(bei)和(he)(he)(he)(he)(he)時(shi)間(jian)(jian)(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)(xian)制(zhi)(zhi)(zhi)(zhi),就等(deng)(deng)著(zhu)(zhu)拿到(dao)數(shu)(shu)據寫論(lun)文。(2)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)數(shu)(shu)據更有(you)(you)(you)(you)(you)保障。嚴格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)質(zhi)(zhi)量(liang)(liang)(liang)(liang)管(guan)(guan)(guan)(guan)(guan)(guan)理(li)(li)(li)(li)(li)體(ti)(ti)(ti)(ti)(ti)系(xi),從(cong)(cong)而(er)(er)保證(zheng)了測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)果(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)真實(shi)(shi)可(ke)(ke)(ke)(ke)靠(kao)。我們還建(jian)(jian)(jian)立了免(mian)費預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)制(zhi)(zhi)(zhi)(zhi)度(du)(du)(du)(du)(du)。(3)防止(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)損(sun)失(shi)無論(lun)是(shi)(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶自(zi)己(ji)完(wan)(wan)(wan)成(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)全(quan)(quan)過(guo)(guo)(guo)程,還是(shi)(shi)(shi)(shi)(shi)(shi)購買試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)完(wan)(wan)(wan)成(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),都(dou)可(ke)(ke)(ke)(ke)能(neng)(neng)(neng)(neng)因(yin)(yin)(yin)為(wei)(wei)(wei)缺(que)乏測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)經驗(yan),導(dao)致(zhi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)失(shi)敗,從(cong)(cong)而(er)(er)損(sun)失(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情況(kuang)。對(dui)(dui)(dui)(dui)于(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)命科學(xue)(xue)研(yan)(yan)究而(er)(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)是(shi)(shi)(shi)(shi)(shi)(shi)＊珍貴的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)旦損(sun)失(shi),輕則造成(cheng)(cheng)研(yan)(yan)究進(jin)(jin)(jin)(jin)度(du)(du)(du)(du)(du)被(bei)耽擱,重(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)則造成(cheng)(cheng)研(yan)(yan)究完(wan)(wan)(wan)全(quan)(quan)失(shi)敗!代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)能(neng)(neng)(neng)(neng)夠充分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)保證(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)功,從(cong)(cong)而(er)(er)防止(zhi)(zhi)(zhi)(zhi)了樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)損(sun)失(shi)。當然,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)質(zhi)(zhi)量(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)功的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前提(ti)(ti)(ti)(ti)(ti)(ti),而(er)(er)且樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)質(zhi)(zhi)量(liang)(liang)(liang)(liang)只有(you)(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶自(zi)己(ji)才(cai)能(neng)(neng)(neng)(neng)控(kong)制(zhi)(zhi)(zhi)(zhi)和(he)(he)(he)(he)(he)保證(zheng)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)(ji)本(ben)(ben)(ben)(ben)原(yuan)(yuan)理(li)(li)(li)(li)(li)??生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he),又稱(cheng)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he),是(shi)(shi)(shi)(shi)(shi)(shi)利用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)特定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)成(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)、動力學(xue)(xue)及化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)反(fan)(fan)應(ying)(ying)等(deng)(deng)技(ji)(ji)術(shu)制(zhi)(zhi)(zhi)(zhi)備(bei)(bei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he),用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)(li)(li)(li)(li)學(xue)(xue)、病(bing)理(li)(li)(li)(li)(li)學(xue)(xue)、藥理(li)(li)(li)(li)(li)學(xue)(xue)等(deng)(deng)領域中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)特定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)或(huo)(huo)(huo)(huo)(huo)(huo)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)包括(kuo)底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)、酶(mei)(mei)(mei)(mei)(mei)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)、探針等(deng)(deng)多個(ge)(ge)組(zu)(zu)(zu)成(cheng)(cheng)部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen),其中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)＊常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)(mei)(mei)法(fa)。底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)(mei)(mei)法(fa)是(shi)(shi)(shi)(shi)(shi)(shi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)(ji)本(ben)(ben)(ben)(ben)原(yuan)(yuan)理(li)(li)(li)(li)(li)。在(zai)(zai)(zai)(zai)該(gai)過(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)與酶(mei)(mei)(mei)(mei)(mei)在(zai)(zai)(zai)(zai)細(xi)(xi)(xi)(xi)(xi)胞(bao)膜上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)結(jie)合(he)(he)形成(cheng)(cheng)基(ji)(ji)質(zhi)(zhi)酶(mei)(mei)(mei)(mei)(mei),進(jin)(jin)(jin)(jin)而(er)(er)形成(cheng)(cheng)底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)(mei)(mei)復(fu)(fu)合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)。隨著(zhu)(zhu)時(shi)間(jian)(jian)(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)推(tui)(tui)移(yi),底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)(mei)(mei)復(fu)(fu)合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)逐漸分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)解(jie)(jie),釋(shi)(shi)放(fang)出(chu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)小的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)大(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)未完(wan)(wan)(wan)全(quan)(quan)消化(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)。未完(wan)(wan)(wan)全(quan)(quan)消化(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)可(ke)(ke)(ke)(ke)以(yi)(yi)被(bei)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)系(xi)列的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)代(dai)謝,同(tong)(tong)(tong)時(shi)底(di)(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)、酶(mei)(mei)(mei)(mei)(mei)和(he)(he)(he)(he)(he)代(dai)謝產物(wu)(wu)(wu)(wu)(wu)(wu)(wu)都(dou)會對(dui)(dui)(dui)(dui)復(fu)(fu)合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)穩定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)產生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)影(ying)響(xiang),從(cong)(cong)而(er)(er)影(ying)響(xiang)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)(guo)(guo)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)可(ke)(ke)(ke)(ke)以(yi)(yi)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)類(lei)型有(you)(you)(you)(you)(you)哪(na)些(xie)(xie)?? ?生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)驗(yan)原(yuan)(yuan)理(li)(li)(li)(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)基(ji)(ji)于(yu)(yu)(yu)(yu)(yu)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)反(fan)(fan)應(ying)(ying),理(li)(li)(li)(li)(li)論(lun)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)(bu)受生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)種(zhong)(zhong)屬及樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)類(lei)型的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)(xian)制(zhi)(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通常(chang)(chang)(chang)只分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)類(lei)型,不(bu)(bu)(bu)(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)種(zhong)(zhong)屬。但(dan)是(shi)(shi)(shi)(shi)(shi)(shi)某(mou)(mou)些(xie)(xie)指(zhi)(zhi)(zhi)(zhi)標在(zai)(zai)(zai)(zai)不(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)種(zhong)(zhong)屬或(huo)(huo)(huo)(huo)(huo)(huo)類(lei)型樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方式不(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)。因(yin)(yin)(yin)此(ci)(ci)(ci),具(ju)體(ti)(ti)(ti)(ti)(ti)情況(kuang)請(qing)參見(jian)(jian)說(shuo)明(ming)(ming)書(shu)(shu)(shu)或(huo)(huo)(huo)(huo)(huo)(huo)與技(ji)(ji)術(shu)支(zhi)持聯(lian)系(xi)確(que)(que)認(ren)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)般要(yao)(yao)(yao)求:1)于(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)＊-好(hao)無溶血(xue)(xue)、脂(zhi)(zhi)血(xue)(xue)、黃-疸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現(xian)象(xiang)。組(zu)(zu)(zu)織(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)(xu)將(jiang)血(xue)(xue)液(ye)(ye)(ye)(ye)(ye)洗(xi)凈擦干后(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)稱(cheng)重(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)處理(li)(li)(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)處理(li)(li)(li)(li)(li)后(hou)(hou)(hou)＊-好(hao)盡快(kuai)進(jin)(jin)(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且保存(cun)(cun)(cun)于(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)盒(he)(he)(he)之(zhi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang),待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)(xu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),若(ruo)不(bu)(bu)(bu)(bu)(bu)(bu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),需(xu)(xu)(xu)(xu)(xu)離(li)(li)心(xin)(xin)后(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特殊指(zhi)(zhi)(zhi)(zhi)標除(chu)外(wai))。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)值(zhi)應(ying)(ying)在(zai)(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)線(xian)(xian)(xian)性(xing)(xing)(xing)(xing)(xing)范(fan)圍(wei)(wei)內,若(ruo)超(chao)過(guo)(guo)(guo)線(xian)(xian)(xian)性(xing)(xing)(xing)(xing)(xing)范(fan)圍(wei)(wei),需(xu)(xu)(xu)(xu)(xu)稀釋(shi)(shi)后(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若(ruo)低(di)于(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)圍(wei)(wei),需(xu)(xu)(xu)(xu)(xu)增加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)濃(nong)度(du)(du)(du)(du)(du)后(hou)(hou)(hou)進(jin)(jin)(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。谷胱甘肽還原(yuan)(yuan)酶(mei)(mei)(mei)(mei)(mei)（GR）測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)本(ben)(ben)(ben)(ben)品(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科研(yan)(yan)實(shi)(shi)驗(yan),不(bu)(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)臨床。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)意(yi)(yi)(yi)義:輔(fu)(fu)酶(mei)(mei)(mei)(mei)(mei)NAD(H)廣泛存(cun)(cun)(cun)在(zai)(zai)(zai)(zai)于(yu)(yu)(yu)(yu)(yu)動物(wu)(wu)(wu)(wu)(wu)(wu)(wu)、植物(wu)(wu)(wu)(wu)(wu)(wu)(wu)、微(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)培養(yang)細(xi)(xi)(xi)(xi)(xi)胞(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),NAD+是(shi)(shi)(shi)(shi)(shi)(shi)糖酵解(jie)(jie)(EMP)和(he)(he)(he)(he)(he)三(san)羧酸(suan)(suan)(suan)(suan)(suan)循環(TCA)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)(yao)(yao)氫受體(ti)(ti)(ti)(ti)(ti),生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?NADH?經呼(hu)吸電子(zi)(zi)鏈(ETC)傳(chuan)遞把電子(zi)(zi)交(jiao)給氧,在(zai)(zai)(zai)(zai)合(he)(he)成(cheng)(cheng)?ATP?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)(tong)(tong)時(shi),形成(cheng)(cheng)大(da)量(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?ROS,同(tong)(tong)(tong)時(shi)?NADH?再(zai)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)為(wei)(wei)(wei)?NAD+。糖、脂(zhi)(zhi)、蛋(dan)(dan)白(bai)(bai)質(zhi)(zhi)三(san)大(da)代(dai)謝物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)解(jie)(jie)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)氧化(hua)(hua)(hua)(hua)(hua)反(fan)(fan)應(ying)(ying)絕大(da)部分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)通過(guo)(guo)(guo)這一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)體(ti)(ti)(ti)(ti)(ti)系(xi)完(wan)(wan)(wan)成(cheng)(cheng)。NAD(H)含(han)(han)(han)量(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)?NADH/NAD+比(bi)值(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)高低(di)可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)評價(jia)糖酵解(jie)(jie)和(he)(he)(he)(he)(he)?TCA?循環的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)強(qiang)弱。較高的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)NAD(H)及?NADH/NAD+比(bi)值(zhi)說(shuo)明(ming)(ming)細(xi)(xi)(xi)(xi)(xi)胞(bao)呼(hu)吸耗氧量(liang)(liang)(liang)(liang)較高,處于(yu)(yu)(yu)(yu)(yu)過(guo)(guo)(guo)氧化(hua)(hua)(hua)(hua)(hua)狀態(tai)(tai)。此(ci)(ci)(ci)外(wai),NADH/NAD+比(bi)值(zhi)升(sheng)高也(ye)可(ke)(ke)(ke)(ke)抑制(zhi)(zhi)(zhi)(zhi)糖酵解(jie)(jie)和(he)(he)(he)(he)(he)?TCA?循環。另外(wai),NAD+降解(jie)(jie)產物(wu)(wu)(wu)(wu)(wu)(wu)(wu)對(dui)(dui)(dui)(dui)細(xi)(xi)(xi)(xi)(xi)胞(bao)信號傳(chuan)導(dao)、代(dai)謝和(he)(he)(he)(he)(he)基(ji)(ji)因(yin)(yin)(yin)表達等(deng)(deng)具(ju)有(you)(you)(you)(you)(you)重(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)要(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)調(diao)(diao)控(kong)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)原(yuan)(yuan)理(li)(li)(li)(li)(li):分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)和(he)(he)(he)(he)(he)堿(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)?NADH,NADH?通過(guo)(guo)(guo)?PMS?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)遞氫作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong),還原(yuan)(yuan)氧化(hua)(hua)(hua)(hua)(hua)型噻唑藍(lan)(MTT)為(wei)(wei)(wei)甲瓚,在(zai)(zai)(zai)(zai)?570nm?下檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)吸光(guang)值(zhi);而(er)(er)?NAD+可(ke)(ke)(ke)(ke)被(bei)乙醇脫氫酶(mei)(mei)(mei)(mei)(mei)還原(yuan)(yuan)為(wei)(wei)(wei)?NADH,進(jin)(jin)(jin)(jin)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)步(bu)采用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)?MTT?還原(yuan)(yuan)法(fa)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。需(xu)(xu)(xu)(xu)(xu)自(zi)備(bei)(bei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)儀(yi)器(qi)(qi)和(he)(he)(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)品(pin)(pin)(pin)(pin):酶(mei)(mei)(mei)(mei)(mei)標儀(yi)、臺式離(li)(li)心(xin)(xin)機、移(yi)液(ye)(ye)(ye)(ye)(ye)器(qi)(qi)、96?孔(kong)板(ban)(ban)、研(yan)(yan)缽、冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)和(he)(he)(he)(he)(he)蒸餾(liu)水(shui)(shui)(shui)。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)(zu)成(cheng)(cheng)和(he)(he)(he)(he)(he)配制(zhi)(zhi)(zhi)(zhi):酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶,4℃保存(cun)(cun)(cun);堿(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶,4℃保存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi):液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?10 mL×1?瓶,4℃保存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)二:液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?3 mL×1?瓶,4℃保存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)三(san):粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶,-20?℃保存(cun)(cun)(cun),用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)時(shi)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)?3mL?蒸餾(liu)水(shui)(shui)(shui),混(hun)(hun)(hun)(hun)勻,用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)(bu)完(wan)(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保存(cun)(cun)(cun)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)周(zhou);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)四:粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶,4?℃保存(cun)(cun)(cun),用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)時(shi)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)?3mL?蒸餾(liu)水(shui)(shui)(shui),混(hun)(hun)(hun)(hun)勻,用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)(bu)完(wan)(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保存(cun)(cun)(cun)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)周(zhou);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)五(wu)(wu):液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?3.6mL×1?瓶,4?℃保存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu)(liu):液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?30mL×1?瓶,4?℃保存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)七:液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶,4?℃保存(cun)(cun)(cun)。NAD+和(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):1?血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(mL):酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(mL)為(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(建(jian)(jian)(jian)議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1mL?血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)?1mL?酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋緊(jin)(jin)(jin),以(yi)(yi)防止(zhi)(zhi)(zhi)(zhi)水(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散失(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻(que)(que)后(hou)(hou)(hou),10000g 4?℃離(li)(li)心(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)?500μL?堿(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he),混(hun)(hun)(hun)(hun)勻,10000g 4?℃離(li)(li)心(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(mL):堿(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(mL)為(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(建(jian)(jian)(jian)議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1mL血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)?1mL?堿(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋緊(jin)(jin)(jin),以(yi)(yi)防止(zhi)(zhi)(zhi)(zhi)水(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散失(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻(que)(que)后(hou)(hou)(hou),10000g 4?℃離(li)(li)心(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)?500μL?酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he),混(hun)(hun)(hun)(hun)勻,10000g 4?℃離(li)(li)心(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。2?組(zu)(zu)(zu)織(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)組(zu)(zu)(zu)織(zhi)(zhi)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(g):酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(mL)為(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(建(jian)(jian)(jian)議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1g?組(zu)(zu)(zu)織(zhi)(zhi),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)?1mL酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)研(yan)(yan)磨,95℃水(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋緊(jin)(jin)(jin),以(yi)(yi)防止(zhi)(zhi)(zhi)(zhi)水(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散失(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻(que)(que)后(hou)(hou)(hou),10000g 4?℃離(li)(li)心(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)?500μL?堿(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he),混(hun)(hun)(hun)(hun)勻,10000g 4?℃離(li)(li)心(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)組(zu)(zu)(zu)織(zhi)(zhi)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(g):堿(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(mL)為(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(建(jian)(jian)(jian)議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1g?組(zu)(zu)(zu)織(zhi)(zhi),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)?1mL堿(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)研(yan)(yan)磨,95℃水(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋緊(jin)(jin)(jin),以(yi)(yi)防止(zhi)(zhi)(zhi)(zhi)水(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散失(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻(que)(que)后(hou)(hou)(hou),10000g 4?℃離(li)(li)心(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)?500μL?酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he),混(hun)(hun)(hun)(hun)勻,10000g 4?℃離(li)(li)心(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3?細(xi)(xi)(xi)(xi)(xi)胞(bao)或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)菌中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先(xian)收(shou)集(ji)細(xi)(xi)(xi)(xi)(xi)胞(bao)或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)菌到(dao)離(li)(li)心(xin)(xin)管(guan)(guan)(guan)(guan)(guan)(guan)內,棄(qi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)(xi)菌或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)數(shu)(shu)量(liang)(liang)(liang)(liang)(104?個(ge)(ge)):酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(mL)為(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(建(jian)(jian)(jian)議(yi)(yi)?500?萬(wan)細(xi)(xi)(xi)(xi)(xi)菌或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)?1mL?酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),超(chao)聲(sheng)(sheng)波破碎(sui)(sui)(冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu),功率(lv)20%或(huo)(huo)(huo)(huo)(huo)(huo)?200W,超(chao)聲(sheng)(sheng)?3s,間(jian)(jian)(jian)隔?10s,重(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)復(fu)(fu)?30?次(ci)(ci)),95℃水(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋緊(jin)(jin)(jin),以(yi)(yi)防止(zhi)(zhi)(zhi)(zhi)水(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散失(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻(que)(que)后(hou)(hou)(hou),10000g 4?℃離(li)(li)心(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)?500μL?堿(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he),混(hun)(hun)(hun)(hun)勻,10000g 4?℃離(li)(li)心(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先(xian)收(shou)集(ji)細(xi)(xi)(xi)(xi)(xi)胞(bao)或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)菌到(dao)離(li)(li)心(xin)(xin)管(guan)(guan)(guan)(guan)(guan)(guan)內,棄(qi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)(xi)菌或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)數(shu)(shu)量(liang)(liang)(liang)(liang)(104?個(ge)(ge)):堿(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(mL)為(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(建(jian)(jian)(jian)議(yi)(yi)?500?萬(wan)細(xi)(xi)(xi)(xi)(xi)菌或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)?1mL?堿(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),超(chao)聲(sheng)(sheng)波破碎(sui)(sui)(冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu),功率(lv)20%或(huo)(huo)(huo)(huo)(huo)(huo)?200W,超(chao)聲(sheng)(sheng)?3s,間(jian)(jian)(jian)隔?10s,重(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)復(fu)(fu)?30?次(ci)(ci)),95℃水(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋緊(jin)(jin)(jin),以(yi)(yi)防止(zhi)(zhi)(zhi)(zhi)水(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散失(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻(que)(que)后(hou)(hou)(hou),10000g 4?℃離(li)(li)心(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)?500μL?酸(suan)(suan)(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he),混(hun)(hun)(hun)(hun)勻,10000g 4?℃離(li)(li)心(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。充分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)混(hun)(hun)(hun)(hun)勻,靜(jing)置(zhi)(zhi)(zhi)?5min?后(hou)(hou)(hou),20000g,25℃離(li)(li)心(xin)(xin)?5min,棄(qi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),沉淀中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru):混(hun)(hun)(hun)(hun)勻,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?200μL?轉移(yi)至(zhi)微(wei)(wei)量(liang)(liang)(liang)(liang)石英(ying)比(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min)或(huo)(huo)(huo)(huo)(huo)(huo)?96?孔(kong)板(ban)(ban)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),570nm?下讀取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)對(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)吸光(guang)值(zhi)?A1?和(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)吸光(guang)值(zhi)?A2,計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)△A=A2-A1。注(zhu)(zhu)(zhu)(zhu)意(yi)(yi)(yi)事項:1、如果(guo)(guo)(guo)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)次(ci)(ci)性(xing)(xing)(xing)(xing)(xing)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)數(shu)(shu)較多,可(ke)(ke)(ke)(ke)將(jiang)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)、二、三(san)和(he)(he)(he)(he)(he)四按(an)(an)(an)(an)比(bi)例(li)配成(cheng)(cheng)混(hun)(hun)(hun)(hun)合(he)(he)液(ye)(ye)(ye)(ye)(ye)。2、對(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)和(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)步(bu)驟的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie):對(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)完(wan)(wan)(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)、二、三(san)、四和(he)(he)(he)(he)(he)五(wu)(wu)后(hou)(hou)(hou)必(bi)(bi)須馬上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu)(liu);測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)完(wan)(wan)(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)、二、三(san)、四和(he)(he)(he)(he)(he)五(wu)(wu)后(hou)(hou)(hou)必(bi)(bi)須反(fan)(fan)應(ying)(ying)?20min?后(hou)(hou)(hou)再(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu)(liu)。3、反(fan)(fan)應(ying)(ying)過(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)注(zhu)(zhu)(zhu)(zhu)意(yi)(yi)(yi)避(bi)光(guang)。4、若(ruo)?NAD+測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0302,NADH?測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0222,說(shuo)明(ming)(ming)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)輔(fu)(fu)酶(mei)(mei)(mei)(mei)(mei)含(han)(han)(han)量(liang)(liang)(liang)(liang)較低(di),已低(di)于(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian)(xian)(xian),可(ke)(ke)(ke)(ke)做如下調(diao)(diao)整(zheng)(zheng):(1)將(jiang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)避(bi)光(guang)靜(jing)置(zhi)(zhi)(zhi)時(shi)間(jian)(jian)(jian)?20min?延(yan)長(chang)到(dao)?60min;(2)在(zai)(zai)(zai)(zai)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)階(jie)段增加(jia)(jia)(jia)(jia)(jia)(jia)(jia)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)(liang)(liang),即取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?0.2g?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)或(huo)(huo)(huo)(huo)(huo)(huo)?0.2mL?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)?1mL?提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)。5、由(you)于(yu)(yu)(yu)(yu)(yu)每(mei)一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)個(ge)(ge)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)需(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)設一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)個(ge)(ge)對(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan),本(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)?100?管(guan)(guan)(guan)(guan)(guan)(guan)保證(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)?48?個(ge)(ge)?NAD+或(huo)(huo)(huo)(huo)(huo)(huo)?NADH.NAD+和(he)(he)(he)(he)(he)?NADH?含(han)(han)(han)量(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan):(一(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)) NAD+含(han)(han)(han)量(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)標準(zhun)條(tiao)件(jian)下的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回歸(gui)曲線(xian)(xian)(xian)為(wei)(wei)(wei)?y = 0.1475x + 0.0302,R2 = 0.9978;其中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)?NAD+濃(nong)度(du)(du)(du)(du)(du)?nmol/mL1、血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)(han)(han)量(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+含(han)(han)(han)量(liang)(liang)(liang)(liang)(nmol/mL)=[(△A -0.0302)÷0.1475×V1) ]÷(V3×V1÷V2)=135.6×(△A -0.0302)2、組(zu)(zu)(zu)織(zhi)(zhi)、細(xi)(xi)(xi)(xi)(xi)菌或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)(han)(han)量(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(1)按(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)蛋(dan)(dan)白(bai)(bai)濃(nong)度(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+(nmol/mg prot)=[(△A -0.0302)÷0.1475×V1)]÷(V1×Cpr)= 6.8×(△A -0.0302)÷Cpr(2)按(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)鮮重(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+(nmol/g?鮮重(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong))= [(△A -0.0302)÷0.1475×V1)]÷(W×V1÷V2)= 13.6×(△A -0.0302)÷W(3)按(an)(an)(an)(an)細(xi)(xi)(xi)(xi)(xi)菌或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)密度(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+(nmol/104 cell)=[(△A -0.0302)÷0.1475×V1)]÷(500×V1÷V2)=0.027×(△A -0.0302)(二)NADH?含(han)(han)(han)量(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)標準(zhun)條(tiao)件(jian)下的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回歸(gui)曲線(xian)(xian)(xian)為(wei)(wei)(wei)?y = 0.1404x + 0.0222,R2 = 0.9976;其中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)?NADH?濃(nong)度(du)(du)(du)(du)(du)?nmol/mL1、血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)(han)(han)量(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH?含(han)(han)(han)量(liang)(liang)(liang)(liang)(nmol/mL)= [ (△A -0.0222)÷0.1404×V1) ]÷(V3×V1÷V2)= 142.5×(△A -0.0222)2、組(zu)(zu)(zu)織(zhi)(zhi)、細(xi)(xi)(xi)(xi)(xi)菌或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)(han)(han)量(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(1)按(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)蛋(dan)(dan)白(bai)(bai)濃(nong)度(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH (nmol/mg prot)= [ (△A -0.0222)÷0.1404×V1) ]÷(V1×Cpr)= 7.1×(△A -0.0222)÷Cpr(2)按(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)鮮重(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH (nmol/g?鮮重(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong))=[(△A -0.0222)÷0.1404×V1) ]÷(W×V1÷V2)= 14.2×(△A -0.0222)÷W(3)按(an)(an)(an)(an)細(xi)(xi)(xi)(xi)(xi)菌或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)密度(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH (nmol/104 cell)= [ (△A -0.0222)÷0.1404×V1) ]÷(500×V1÷V2)=0.028×(△A -0.0222)V1:加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)反(fan)(fan)應(ying)(ying)體(ti)(ti)(ti)(ti)(ti)系(xi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji),0.02mL;V2:加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji),2mL;V3:加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji):0.1mL;Cpr:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)蛋(dan)(dan)白(bai)(bai)質(zhi)(zhi)濃(nong)度(du)(du)(du)(du)(du),mg/mL; W:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)質(zhi)(zhi)量(liang)(liang)(liang)(liang),g;500:細(xi)(xi)(xi)(xi)(xi)胞(bao)或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)菌總數(shu)(shu),500?萬(wan)。注(zhu)(zhu)(zhu)(zhu)?意(yi)(yi)(yi):?檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian)(xian)(xian)為(wei)(wei)(wei)?0.1nmol/mL?或(huo)(huo)(huo)(huo)(huo)(huo)?0.1nmol/g?鮮重(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?或(huo)(huo)(huo)(huo)(huo)(huo)?0.001nmol/mg prot
谷胱甘肽S-轉(zhuan)(zhuan)移酶(mei)(mei)(mei)（GST）測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)科研(yan)實(shi)驗(yan)(yan),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)臨(lin)床。生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)可(ke)(ke)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類型有(you)(you)(you)哪(na)(na)些(xie)(xie)???生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗(yan)(yan)原(yuan)理(li)(li)(li)是(shi)(shi)(shi)(shi)基(ji)(ji)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)化(hua)(hua)(hua)(hua)(hua)(hua)學反(fan)(fan)應(ying)(ying),理(li)(li)(li)論上(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)受(shou)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)種(zhong)屬(shu)及(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類型的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)制(zhi)(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通(tong)常(chang)(chang)只(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類型,不(bu)(bu)(bu)(bu)(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)種(zhong)屬(shu)。但(dan)是(shi)(shi)(shi)(shi)某些(xie)(xie)指(zhi)(zhi)(zhi)標(biao)(biao)在(zai)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)種(zhong)屬(shu)或(huo)(huo)(huo)(huo)(huo)類型樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方式(shi)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)。因(yin)此(ci),具體(ti)(ti)(ti)(ti)(ti)情況(kuang)請參見(jian)說明書(shu)或(huo)(huo)(huo)(huo)(huo)與技(ji)(ji)(ji)術(shu)支持(chi)聯(lian)系(xi)(xi)(xi)確(que)認(ren)(ren)。生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)(yi)般要(yao)(yao)(yao)求:1)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)無(wu)(wu)(wu)溶(rong)血(xue)(xue)、脂(zhi)血(xue)(xue)、的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現(xian)象(xiang)(xiang)。組(zu)織(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)將血(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)洗凈(jing)擦干后(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)稱重(zhong)處(chu)(chu)(chu)理(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)處(chu)(chu)(chu)理(li)(li)(li)后(hou)(hou)(hou)(hou)(hou)盡快進(jin)(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且(qie)(qie)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)(bing)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)之(zhi)上(shang)(shang)(shang)(shang),待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。求3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),若不(bu)(bu)(bu)(bu)(bu)(bu)(bu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),需(xu)離(li)(li)(li)(li)心(xin)(xin)后(hou)(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特(te)殊指(zhi)(zhi)(zhi)標(biao)(biao)除外(wai)(wai))。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)值應(ying)(ying)在(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)線(xian)(xian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)(fan)圍(wei)內,若超(chao)過(guo)(guo)(guo)線(xian)(xian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)(fan)圍(wei),需(xu)稀釋后(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若低于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)(fan)(fan)圍(wei),需(xu)增(zeng)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)濃度(du)(du)(du)后(hou)(hou)(hou)(hou)(hou)進(jin)(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。微(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)法(fa)和(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)光(guang)度(du)(du)(du)法(fa)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區(qu)別(bie)?分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)光(guang)度(du)(du)(du)法(fa):主(zhu)流(liu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi)為(wei)(wei)(wei)1mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)(yi)器(qi)(qi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)光(guang)度(du)(du)(du)計(ji)(ji)(ji)(ji)(ji),能(neng)(neng)(neng)(neng)夠(gou)(gou)(gou)大限(xian)(xian)度(du)(du)(du)地滿足絕(jue)大多(duo)數(shu)(shu)(shu)(shu)用(yong)(yong)(yong)(yong)戶(hu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)需(xu)要(yao)(yao)(yao)。微(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)法(fa):主(zhu)流(liu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi)為(wei)(wei)(wei)0.2mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)(yi)器(qi)(qi)或(huo)(huo)(huo)(huo)(huo)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)光(guang)度(du)(du)(du)計(ji)(ji)(ji)(ji)(ji),測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)更加(jia)(jia)(jia)簡便快捷。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)規格(ge)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)是(shi)(shi)(shi)(shi)100管(guan)(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)50管(guan)(guan)(guan)(guan)是(shi)(shi)(shi)(shi)什(shen)么(me)(me)意(yi)(yi)思?(1)50管(guan)(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):50管(guan)(guan)(guan)(guan)指(zhi)(zhi)(zhi)能(neng)(neng)(neng)(neng)夠(gou)(gou)(gou)做(zuo)50次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)(zhi)(zhi)可(ke)(ke)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)48個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)。如(ru)(ru)(ru)(ru)果每(mei)個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)重(zhong)復(fu)(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci)(ci)(ci),那么(me)(me)只(zhi)(zhi)能(neng)(neng)(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)16個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)。其(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)2次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)空白(bai)管(guan)(guan)(guan)(guan)或(huo)(huo)(huo)(huo)(huo)者對(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)。值得特(te)別(bie)注(zhu)(zhu)意(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi),50管(guan)(guan)(guan)(guan)/24樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)每(mei)個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)需(xu)要(yao)(yao)(yao)做(zuo)對(dui)(dui)照(zhao)(zhao)(zhao)(zhao),因(yin)此(ci)能(neng)(neng)(neng)(neng)夠(gou)(gou)(gou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)僅(jin)為(wei)(wei)(wei)8個(ge)(ge)(ge)(假設(she)做(zuo)3次(ci)(ci)(ci)(ci)重(zhong)復(fu)(fu))(2)100管(guan)(guan)(guan)(guan)/96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):100管(guan)(guan)(guan)(guan)指(zhi)(zhi)(zhi)能(neng)(neng)(neng)(neng)夠(gou)(gou)(gou)做(zuo)100次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)(zhi)(zhi)可(ke)(ke)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)96個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin),如(ru)(ru)(ru)(ru)果每(mei)個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)重(zhong)復(fu)(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci)(ci)(ci),那么(me)(me)只(zhi)(zhi)能(neng)(neng)(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)32個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)。其(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)4次(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)空白(bai)管(guan)(guan)(guan)(guan)或(huo)(huo)(huo)(huo)(huo)者對(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)。注(zhu)(zhu)意(yi)(yi)由(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)瓶(ping)(ping)璧和(he)(he)(he)(he)(he)(he)(he)移液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)器(qi)(qi)吸附等(deng)(deng),通(tong)常(chang)(chang)要(yao)(yao)(yao)比(bi)(bi)(bi)理(li)(li)(li)論測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)次(ci)(ci)(ci)(ci)數(shu)(shu)(shu)(shu)少1~3次(ci)(ci)(ci)(ci)。其(qi)它規格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)(han)(han)(han)義依次(ci)(ci)(ci)(ci)類推。值得特(te)別(bie)注(zhu)(zhu)意(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi),100管(guan)(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)每(mei)個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)需(xu)要(yao)(yao)(yao)做(zuo)對(dui)(dui)照(zhao)(zhao)(zhao)(zhao),因(yin)此(ci)能(neng)(neng)(neng)(neng)夠(gou)(gou)(gou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)僅(jin)為(wei)(wei)(wei)16個(ge)(ge)(ge)(假設(she)做(zuo)3次(ci)(ci)(ci)(ci)重(zhong)復(fu)(fu))。如(ru)(ru)(ru)(ru)何防(fang)止(zhi)(zhi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)失(shi)(shi)(shi)(shi)效(xiao)?????不(bu)(bu)(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)過(guo)(guo)(guo)早訂(ding)購,以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)免(mian)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)失(shi)(shi)(shi)(shi)效(xiao).收(shou)到(dao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)后(hou)(hou)(hou)(hou)(hou),檢(jian)(jian)(jian)(jian)(jian)(jian)查外(wai)(wai)包裝是(shi)(shi)(shi)(shi)否正(zheng)常(chang)(chang)。馬(ma)上(shang)(shang)(shang)(shang)打開,按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)說明書(shu)核(he)對(dui)(dui)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)數(shu)(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)否正(zheng)確(que),注(zhu)(zhu)意(yi)(yi)瓶(ping)(ping)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)狀(zhuang)態(tai)是(shi)(shi)(shi)(shi)否與說明書(shu)一(yi)(yi)(yi)(yi)(yi)(yi)致(zhi)。如(ru)(ru)(ru)(ru)果有(you)(you)(you)問(wen)題,或(huo)(huo)(huo)(huo)(huo)者懷疑,馬(ma)上(shang)(shang)(shang)(shang)聯(lian)系(xi)(xi)(xi)公(gong)司銷售(shou),進(jin)(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)確(que)認(ren)(ren)。如(ru)(ru)(ru)(ru)果沒有(you)(you)(you)問(wen)題,請務必按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)說明書(shu)進(jin)(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。注(zhu)(zhu)意(yi)(yi),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji),可(ke)(ke)能(neng)(neng)(neng)(neng)需(xu)要(yao)(yao)(yao)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)在(zai)常(chang)(chang)溫(wen)(25°℃)、4℃和(he)(he)(he)(he)(he)(he)(he)-20℃,務必分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)要(yao)(yao)(yao)求保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)整(zheng)個(ge)(ge)(ge)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)放置(zhi)(zhi)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)(bing)箱。如(ru)(ru)(ru)(ru)何進(jin)(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)預測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)?(1)務必在(zai)7個(ge)(ge)(ge)工作(zuo)日內進(jin)(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)預測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)確(que)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)該試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)是(shi)(shi)(shi)(shi)否適合您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin),以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)免(mian)造(zao)成(cheng)(cheng)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)和(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浪費。(2)選(xuan)擇(ze)2~3個(ge)(ge)(ge)預期差異(yi)(yi)比(bi)(bi)(bi)較(jiao)大的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin),嚴格(ge)按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)說明書(shu)進(jin)(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)操(cao)作(zuo),注(zhu)(zhu)意(yi)(yi)儀(yi)(yi)器(qi)(qi)工作(zuo)狀(zhuang)態(tai),操(cao)作(zuo)規范(fan)(fan)(fan),控制(zhi)(zhi)(zhi)(zhi)好(hao)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)條件(jian),尤其(qi)是(shi)(shi)(shi)(shi)溫(wen)度(du)(du)(du)。(3)如(ru)(ru)(ru)(ru)實(shi)與公(gong)司銷售(shou)溝通(tong)預測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)(jie)果,公(gong)司技(ji)(ji)(ji)術(shu)人員會確(que)認(ren)(ren)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)(jie)果是(shi)(shi)(shi)(shi)否正(zheng)常(chang)(chang),是(shi)(shi)(shi)(shi)否需(xu)要(yao)(yao)(yao)調整(zheng),如(ru)(ru)(ru)(ru)何進(jin)(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)調整(zheng),從而(er)(er)確(que)保(bao)(bao)(bao)(bao)(bao)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)(jie)果可(ke)(ke)靠(kao)。比(bi)(bi)(bi)色皿有(you)(you)(you)哪(na)(na)些(xie)(xie)規格(ge)?(1)常(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)比(bi)(bi)(bi)色皿,如(ru)(ru)(ru)(ru)1mL和(he)(he)(he)(he)(he)(he)(he)3mL等(deng)(deng),常(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)法(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)都(dou)是(shi)(shi)(shi)(shi)選(xuan)用(yong)(yong)(yong)(yong)1mL體(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi),只(zhi)(zhi)能(neng)(neng)(neng)(neng)用(yong)(yong)(yong)(yong)1mL比(bi)(bi)(bi)色皿,不(bu)(bu)(bu)(bu)(bu)(bu)(bu)可(ke)(ke)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)用(yong)(yong)(yong)(yong)更大體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)色皿,否則試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)量(liang)(liang)(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)夠(gou)(gou)(gou),無(wu)(wu)(wu)法(fa)正(zheng)常(chang)(chang)比(bi)(bi)(bi)色。(2)微(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)比(bi)(bi)(bi)色皿,如(ru)(ru)(ru)(ru)0.25mL和(he)(he)(he)(he)(he)(he)(he)0.5mL等(deng)(deng),微(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)法(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)都(dou)是(shi)(shi)(shi)(shi)選(xuan)用(yong)(yong)(yong)(yong)0.25mL比(bi)(bi)(bi)色皿。一(yi)(yi)(yi)(yi)(yi)(yi)般客(ke)戶(hu)都(dou)選(xuan)擇(ze)使用(yong)(yong)(yong)(yong)標(biao)(biao)儀(yi)(yi)(需(xu)要(yao)(yao)(yao)自(zi)備酶(mei)(mei)(mei)標(biao)(biao)板)(3)紫(zi)外(wai)(wai)波長檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)需(xu)要(yao)(yao)(yao)石英比(bi)(bi)(bi)色皿,不(bu)(bu)(bu)(bu)(bu)(bu)(bu)可(ke)(ke)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)用(yong)(yong)(yong)(yong)普通(tong)玻璃比(bi)(bi)(bi)色皿。(4)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)規格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)色皿,其(qi)外(wai)(wai)觀大小(xiao)是(shi)(shi)(shi)(shi)一(yi)(yi)(yi)(yi)(yi)(yi)致(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de),都(dou)是(shi)(shi)(shi)(shi)1cm光(guang)徑(jing)(也有(you)(you)(you)0.5cm光(guang)徑(jing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)色皿,不(bu)(bu)(bu)(bu)(bu)(bu)(bu)常(chang)(chang)見(jian),也可(ke)(ke)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)用(yong)(yong)(yong)(yong),但(dan)是(shi)(shi)(shi)(shi)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)時要(yao)(yao)(yao)注(zhu)(zhu)意(yi)(yi)修改計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)公(gong)式(shi)),其(qi)內容(rong)積(ji)(ji)(ji)(ji)(ji)差異(yi)(yi)在(zai)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)壁的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)厚(hou)度(du)(du)(du)。代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)優點(dian)(1)更加(jia)(jia)(jia)省(sheng)事兒,完(wan)(wan)(wan)全(quan)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)必為(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)犯難。用(yong)(yong)(yong)(yong)戶(hu)只(zhi)(zhi)要(yao)(yao)(yao)提(ti)(ti)(ti)(ti)(ti)(ti)供(gong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)和(he)(he)(he)(he)(he)(he)(he)想(xiang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)指(zhi)(zhi)(zhi)標(biao)(biao),就(jiu)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)再(zai)需(xu)要(yao)(yao)(yao)為(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)操(cao)心(xin)(xin)了(le)(le),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)再(zai)受(shou)到(dao)操(cao)作(zuo)技(ji)(ji)(ji)能(neng)(neng)(neng)(neng)、儀(yi)(yi)器(qi)(qi)設(she)備和(he)(he)(he)(he)(he)(he)(he)時間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)制(zhi)(zhi)(zhi)(zhi),就(jiu)等(deng)(deng)著拿到(dao)數(shu)(shu)(shu)(shu)據(ju)寫(xie)論文。(2)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)數(shu)(shu)(shu)(shu)據(ju)更有(you)(you)(you)保(bao)(bao)(bao)(bao)(bao)障。嚴格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)管(guan)(guan)(guan)(guan)理(li)(li)(li)體(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi),從而(er)(er)保(bao)(bao)(bao)(bao)(bao)證(zheng)了(le)(le)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)(jie)果的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)真實(shi)可(ke)(ke)靠(kao)。我(wo)們還(huan)建(jian)立了(le)(le)免(mian)費預測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)制(zhi)(zhi)(zhi)(zhi)度(du)(du)(du)。(3)防(fang)止(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)損失(shi)(shi)(shi)(shi)無(wu)(wu)(wu)論是(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)戶(hu)自(zi)己完(wan)(wan)(wan)成(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)全(quan)過(guo)(guo)(guo)程,還(huan)是(shi)(shi)(shi)(shi)購買(mai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)完(wan)(wan)(wan)成(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),都(dou)可(ke)(ke)能(neng)(neng)(neng)(neng)因(yin)為(wei)(wei)(wei)缺乏測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)經(jing)驗(yan)(yan),導致(zhi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)失(shi)(shi)(shi)(shi)敗,從而(er)(er)損失(shi)(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情況(kuang)。對(dui)(dui)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)命科學研(yan)究(jiu)而(er)(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)是(shi)(shi)(shi)(shi)＊珍貴的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)一(yi)(yi)(yi)(yi)(yi)(yi)旦(dan)損失(shi)(shi)(shi)(shi),輕則造(zao)成(cheng)(cheng)研(yan)究(jiu)進(jin)(jin)(jin)(jin)(jin)(jin)度(du)(du)(du)被(bei)耽(dan)擱,重(zhong)則造(zao)成(cheng)(cheng)研(yan)究(jiu)完(wan)(wan)(wan)全(quan)失(shi)(shi)(shi)(shi)敗!代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)能(neng)(neng)(neng)(neng)夠(gou)(gou)(gou)充(chong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)保(bao)(bao)(bao)(bao)(bao)證(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)功,從而(er)(er)防(fang)止(zhi)(zhi)了(le)(le)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)損失(shi)(shi)(shi)(shi)。當然,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)功的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前提(ti)(ti)(ti)(ti)(ti)(ti),而(er)(er)且(qie)(qie)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)只(zhi)(zhi)有(you)(you)(you)用(yong)(yong)(yong)(yong)戶(hu)自(zi)己才能(neng)(neng)(neng)(neng)控制(zhi)(zhi)(zhi)(zhi)和(he)(he)(he)(he)(he)(he)(he)保(bao)(bao)(bao)(bao)(bao)證(zheng)。生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)(ji)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)原(yuan)理(li)(li)(li)??生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he),又稱生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he),是(shi)(shi)(shi)(shi)利用(yong)(yong)(yong)(yong)特(te)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)成(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)、動力學及(ji)(ji)化(hua)(hua)(hua)(hua)(hua)(hua)學反(fan)(fan)應(ying)(ying)等(deng)(deng)技(ji)(ji)(ji)術(shu)制(zhi)(zhi)(zhi)(zhi)備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he),用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)生(sheng)(sheng)(sheng)(sheng)(sheng)理(li)(li)(li)學、病(bing)理(li)(li)(li)學、藥理(li)(li)(li)學等(deng)(deng)領域中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)特(te)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)或(huo)(huo)(huo)(huo)(huo)化(hua)(hua)(hua)(hua)(hua)(hua)學物(wu)(wu)(wu)質(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)包括底物(wu)(wu)(wu)、酶(mei)(mei)(mei)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)、探針等(deng)(deng)多(duo)個(ge)(ge)(ge)組(zu)成(cheng)(cheng)部(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen),其(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)＊常(chang)(chang)用(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)底物(wu)(wu)(wu)-酶(mei)(mei)(mei)法(fa)。底物(wu)(wu)(wu)-酶(mei)(mei)(mei)法(fa)是(shi)(shi)(shi)(shi)生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)(ji)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)原(yuan)理(li)(li)(li)。在(zai)該過(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),底物(wu)(wu)(wu)與酶(mei)(mei)(mei)在(zai)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)膜上(shang)(shang)(shang)(shang)結(jie)(jie)合形成(cheng)(cheng)基(ji)(ji)質(zhi)(zhi)(zhi)酶(mei)(mei)(mei),進(jin)(jin)(jin)(jin)(jin)(jin)而(er)(er)形成(cheng)(cheng)底物(wu)(wu)(wu)-酶(mei)(mei)(mei)復(fu)(fu)合物(wu)(wu)(wu)。隨著時間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)推移,底物(wu)(wu)(wu)-酶(mei)(mei)(mei)復(fu)(fu)合物(wu)(wu)(wu)逐漸分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)解(jie),釋放出分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)小(xiao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底物(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)大的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)未(wei)完(wan)(wan)(wan)全(quan)消(xiao)化(hua)(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底物(wu)(wu)(wu)。未(wei)完(wan)(wan)(wan)全(quan)消(xiao)化(hua)(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底物(wu)(wu)(wu)可(ke)(ke)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)被(bei)一(yi)(yi)(yi)(yi)(yi)(yi)系(xi)(xi)(xi)列的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)代(dai)謝(xie)(xie),同(tong)(tong)時底物(wu)(wu)(wu)、酶(mei)(mei)(mei)和(he)(he)(he)(he)(he)(he)(he)代(dai)謝(xie)(xie)產(chan)物(wu)(wu)(wu)都(dou)會對(dui)(dui)復(fu)(fu)合物(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)穩定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)產(chan)生(sheng)(sheng)(sheng)(sheng)(sheng)影響,從而(er)(er)影響檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)果。生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)可(ke)(ke)以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類型有(you)(you)(you)哪(na)(na)些(xie)(xie)?? ?生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗(yan)(yan)原(yuan)理(li)(li)(li)是(shi)(shi)(shi)(shi)基(ji)(ji)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)化(hua)(hua)(hua)(hua)(hua)(hua)學反(fan)(fan)應(ying)(ying),理(li)(li)(li)論上(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)受(shou)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)種(zhong)屬(shu)及(ji)(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類型的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)制(zhi)(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通(tong)常(chang)(chang)只(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類型,不(bu)(bu)(bu)(bu)(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)種(zhong)屬(shu)。但(dan)是(shi)(shi)(shi)(shi)某些(xie)(xie)指(zhi)(zhi)(zhi)標(biao)(biao)在(zai)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)種(zhong)屬(shu)或(huo)(huo)(huo)(huo)(huo)類型樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方式(shi)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)。因(yin)此(ci),具體(ti)(ti)(ti)(ti)(ti)情況(kuang)請參見(jian)說明書(shu)或(huo)(huo)(huo)(huo)(huo)與技(ji)(ji)(ji)術(shu)支持(chi)聯(lian)系(xi)(xi)(xi)確(que)認(ren)(ren)。生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)(yi)般要(yao)(yao)(yao)求:1)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)＊-好(hao)無(wu)(wu)(wu)溶(rong)血(xue)(xue)、脂(zhi)血(xue)(xue)、黃(huang)-疸(dan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現(xian)象(xiang)(xiang)。組(zu)織(zhi)(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)將血(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)洗凈(jing)擦干后(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)稱重(zhong)處(chu)(chu)(chu)理(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)處(chu)(chu)(chu)理(li)(li)(li)后(hou)(hou)(hou)(hou)(hou)＊-好(hao)盡快進(jin)(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且(qie)(qie)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)(bing)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)之(zhi)上(shang)(shang)(shang)(shang),待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),若不(bu)(bu)(bu)(bu)(bu)(bu)(bu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),需(xu)離(li)(li)(li)(li)心(xin)(xin)后(hou)(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特(te)殊指(zhi)(zhi)(zhi)標(biao)(biao)除外(wai)(wai))。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)值應(ying)(ying)在(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)線(xian)(xian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)(fan)圍(wei)內,若超(chao)過(guo)(guo)(guo)線(xian)(xian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)(fan)圍(wei),需(xu)稀釋后(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若低于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)(fan)(fan)圍(wei),需(xu)增(zeng)加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)濃度(du)(du)(du)后(hou)(hou)(hou)(hou)(hou)進(jin)(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。谷胱甘肽S-轉(zhuan)(zhuan)移酶(mei)(mei)(mei)（GST）測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)科研(yan)實(shi)驗(yan)(yan),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)臨(lin)床。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)意(yi)(yi)義:輔酶(mei)(mei)(mei)NAD(H)廣泛存(cun)(cun)(cun)在(zai)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)動物(wu)(wu)(wu)、植物(wu)(wu)(wu)、微(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)培養細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),NAD+是(shi)(shi)(shi)(shi)糖(tang)酵(jiao)解(jie)(EMP)和(he)(he)(he)(he)(he)(he)(he)三(san)羧酸循環(huan)(TCA)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要(yao)(yao)(yao)氫(qing)(qing)受(shou)體(ti)(ti)(ti)(ti)(ti),生(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?NADH?經(jing)呼吸電子(zi)鏈(ETC)傳遞(di)把電子(zi)交給氧(yang),在(zai)合成(cheng)(cheng)?ATP?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)(tong)時,形成(cheng)(cheng)大量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?ROS,同(tong)(tong)時?NADH?再(zai)生(sheng)(sheng)(sheng)(sheng)(sheng)為(wei)(wei)(wei)?NAD+。糖(tang)、脂(zhi)、蛋白(bai)質(zhi)(zhi)(zhi)三(san)大代(dai)謝(xie)(xie)物(wu)(wu)(wu)質(zhi)(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)解(jie)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)氧(yang)化(hua)(hua)(hua)(hua)(hua)(hua)反(fan)(fan)應(ying)(ying)絕(jue)大部(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)通(tong)過(guo)(guo)(guo)這一(yi)(yi)(yi)(yi)(yi)(yi)體(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi)完(wan)(wan)(wan)成(cheng)(cheng)。NAD(H)含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)(he)(he)?NADH/NAD+比(bi)(bi)(bi)值的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)高(gao)低可(ke)(ke)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)評價糖(tang)酵(jiao)解(jie)和(he)(he)(he)(he)(he)(he)(he)?TCA?循環(huan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)強(qiang)弱。較(jiao)高(gao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)NAD(H)及(ji)(ji)?NADH/NAD+比(bi)(bi)(bi)值說明細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)呼吸耗氧(yang)量(liang)(liang)(liang)(liang)(liang)(liang)較(jiao)高(gao),處(chu)(chu)(chu)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)過(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)(hua)(hua)狀(zhuang)態(tai)。此(ci)外(wai)(wai),NADH/NAD+比(bi)(bi)(bi)值升高(gao)也可(ke)(ke)抑(yi)制(zhi)(zhi)(zhi)(zhi)糖(tang)酵(jiao)解(jie)和(he)(he)(he)(he)(he)(he)(he)?TCA?循環(huan)。另外(wai)(wai),NAD+降解(jie)產(chan)物(wu)(wu)(wu)對(dui)(dui)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)信號傳導、代(dai)謝(xie)(xie)和(he)(he)(he)(he)(he)(he)(he)基(ji)(ji)因(yin)表達(da)等(deng)(deng)具有(you)(you)(you)重(zhong)要(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)調控作(zuo)用(yong)(yong)(yong)(yong)。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)原(yuan)理(li)(li)(li):分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)用(yong)(yong)(yong)(yong)酸性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)和(he)(he)(he)(he)(he)(he)(he)堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH,NADH?通(tong)過(guo)(guo)(guo)?PMS?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)遞(di)氫(qing)(qing)作(zuo)用(yong)(yong)(yong)(yong),還(huan)原(yuan)氧(yang)化(hua)(hua)(hua)(hua)(hua)(hua)型噻唑藍(MTT)為(wei)(wei)(wei)甲(jia)瓚,在(zai)?570nm?下檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)吸光(guang)值;而(er)(er)?NAD+可(ke)(ke)被(bei)乙(yi)醇脫氫(qing)(qing)酶(mei)(mei)(mei)還(huan)原(yuan)為(wei)(wei)(wei)?NADH,進(jin)(jin)(jin)(jin)(jin)(jin)一(yi)(yi)(yi)(yi)(yi)(yi)步(bu)采用(yong)(yong)(yong)(yong)?MTT?還(huan)原(yuan)法(fa)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。需(xu)自(zi)備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)儀(yi)(yi)器(qi)(qi)和(he)(he)(he)(he)(he)(he)(he)用(yong)(yong)(yong)(yong)品(pin)(pin)(pin)(pin)(pin)(pin):酶(mei)(mei)(mei)標(biao)(biao)儀(yi)(yi)、臺(tai)式(shi)離(li)(li)(li)(li)心(xin)(xin)機(ji)、移液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)器(qi)(qi)、96?孔板、研(yan)缽、冰(bing)(bing)(bing)(bing)(bing)和(he)(he)(he)(he)(he)(he)(he)蒸餾水(shui)(shui)(shui)(shui)。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)成(cheng)(cheng)和(he)(he)(he)(he)(he)(he)(he)配(pei)制(zhi)(zhi)(zhi)(zhi):酸性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping),4℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun);堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping),4℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)(yi):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?10 mL×1?瓶(ping)(ping),4℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)二(er):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?3 mL×1?瓶(ping)(ping),4℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)三(san):粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping)(ping),-20?℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun),用(yong)(yong)(yong)(yong)時加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?3mL?蒸餾水(shui)(shui)(shui)(shui),混(hun)(hun)勻,用(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)完(wan)(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)一(yi)(yi)(yi)(yi)(yi)(yi)周(zhou);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)四(si):粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping)(ping),4?℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun),用(yong)(yong)(yong)(yong)時加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?3mL?蒸餾水(shui)(shui)(shui)(shui),混(hun)(hun)勻,用(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)完(wan)(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)一(yi)(yi)(yi)(yi)(yi)(yi)周(zhou);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)五(wu):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?3.6mL×1?瓶(ping)(ping),4?℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六:液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?30mL×1?瓶(ping)(ping),4?℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)七(qi):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping),4?℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):1?血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(mL):酸性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(建(jian)議取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1mL?血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)(jiang)),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL?酸性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)(yu)?5min(蓋緊(jin),以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)防(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻(que)后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻,10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(mL):堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(建(jian)議取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1mL血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)(jiang)),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)(yu)?5min(蓋緊(jin),以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)防(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻(que)后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?酸性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻,10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。2?組(zu)織(zhi)(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)組(zu)織(zhi)(zhi)(zhi)(zhi)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(g):酸性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(建(jian)議取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1g?組(zu)織(zhi)(zhi)(zhi)(zhi),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL酸性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu)研(yan)磨,95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)(yu)?5min(蓋緊(jin),以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)防(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻(que)后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻,10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)組(zu)織(zhi)(zhi)(zhi)(zhi)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(g):堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(建(jian)議取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1g?組(zu)織(zhi)(zhi)(zhi)(zhi),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu)研(yan)磨,95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)(yu)?5min(蓋緊(jin),以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)防(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻(que)后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?酸性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻,10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3?細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先收(shou)集細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)到(dao)離(li)(li)(li)(li)心(xin)(xin)管(guan)(guan)(guan)(guan)內,棄(qi)(qi)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)數(shu)(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)):酸性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(建(jian)議?500?萬細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL?酸性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),超(chao)聲波破(po)碎(冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu),功率20%或(huo)(huo)(huo)(huo)(huo)?200W,超(chao)聲?3s,間(jian)隔?10s,重(zhong)復(fu)(fu)?30?次(ci)(ci)(ci)(ci)),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)(yu)?5min(蓋緊(jin),以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)防(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻(que)后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻,10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先收(shou)集細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)到(dao)離(li)(li)(li)(li)心(xin)(xin)管(guan)(guan)(guan)(guan)內,棄(qi)(qi)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)數(shu)(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)):堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)例(li)(建(jian)議?500?萬細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),超(chao)聲波破(po)碎(冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu),功率20%或(huo)(huo)(huo)(huo)(huo)?200W,超(chao)聲?3s,間(jian)隔?10s,重(zhong)復(fu)(fu)?30?次(ci)(ci)(ci)(ci)),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)(yu)?5min(蓋緊(jin),以(yi)(yi)(yi)(yi)(yi)(yi)(yi)(yi)防(fang)止(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻(que)后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?酸性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻,10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。充(chong)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)混(hun)(hun)勻,靜置(zhi)(zhi)?5min?后(hou)(hou)(hou)(hou)(hou),20000g,25℃離(li)(li)(li)(li)心(xin)(xin)?5min,棄(qi)(qi)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),沉淀(dian)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)入(ru)(ru)(ru)(ru):混(hun)(hun)勻,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?200μL?轉(zhuan)(zhuan)移至微(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)石英比(bi)(bi)(bi)色皿或(huo)(huo)(huo)(huo)(huo)?96?孔板中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),570nm?下讀取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)對(dui)(dui)照(zhao)(zhao)(zhao)(zhao)吸光(guang)值?A1?和(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)吸光(guang)值?A2,計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)△A=A2-A1。注(zhu)(zhu)意(yi)(yi)事項(xiang):1、如(ru)(ru)(ru)(ru)果一(yi)(yi)(yi)(yi)(yi)(yi)次(ci)(ci)(ci)(ci)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)數(shu)(shu)(shu)(shu)較(jiao)多(duo),可(ke)(ke)將試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)(yi)、二(er)、三(san)和(he)(he)(he)(he)(he)(he)(he)四(si)按(an)(an)(an)(an)比(bi)(bi)(bi)例(li)配(pei)成(cheng)(cheng)混(hun)(hun)合液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。2、對(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)步(bu)驟的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區(qu)別(bie):對(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)加(jia)(jia)(jia)完(wan)(wan)(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)(yi)、二(er)、三(san)、四(si)和(he)(he)(he)(he)(he)(he)(he)五(wu)后(hou)(hou)(hou)(hou)(hou)必須馬(ma)上(shang)(shang)(shang)(shang)加(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六;測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)加(jia)(jia)(jia)完(wan)(wan)(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)(yi)、二(er)、三(san)、四(si)和(he)(he)(he)(he)(he)(he)(he)五(wu)后(hou)(hou)(hou)(hou)(hou)必須反(fan)(fan)應(ying)(ying)?20min?后(hou)(hou)(hou)(hou)(hou)再(zai)加(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六。3、反(fan)(fan)應(ying)(ying)過(guo)(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)注(zhu)(zhu)意(yi)(yi)避光(guang)。4、若?NAD+測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0302,NADH?測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0222,說明樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)輔酶(mei)(mei)(mei)含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)較(jiao)低,已低于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian)(xian),可(ke)(ke)做(zuo)如(ru)(ru)(ru)(ru)下調整(zheng):(1)將測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)避光(guang)靜置(zhi)(zhi)時間(jian)?20min?延長到(dao)?60min;(2)在(zai)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)階(jie)段增(zeng)加(jia)(jia)(jia)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)(liang)(liang)(liang)(liang),即取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?0.2g?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)或(huo)(huo)(huo)(huo)(huo)?0.2mL?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL?提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。5、由(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)每(mei)一(yi)(yi)(yi)(yi)(yi)(yi)個(ge)(ge)(ge)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)需(xu)要(yao)(yao)(yao)設(she)一(yi)(yi)(yi)(yi)(yi)(yi)個(ge)(ge)(ge)對(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan),本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?100?管(guan)(guan)(guan)(guan)保(bao)(bao)(bao)(bao)(bao)證(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)?48?個(ge)(ge)(ge)?NAD+或(huo)(huo)(huo)(huo)(huo)?NADH.NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH?含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan):(一(yi)(yi)(yi)(yi)(yi)(yi)) NAD+含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)標(biao)(biao)準條件(jian)下的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回歸曲(qu)線(xian)(xian)為(wei)(wei)(wei)?y = 0.1475x + 0.0302,R2 = 0.9978;其(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)?NAD+濃度(du)(du)(du)?nmol/mL1、血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)NAD+含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)=[(△A -0.0302)÷0.1475×V1) ]÷(V3×V1÷V2)=135.6×(△A -0.0302)2、組(zu)織(zhi)(zhi)(zhi)(zhi)、細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(1)按(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋白(bai)濃度(du)(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)NAD+(nmol/mg prot)=[(△A -0.0302)÷0.1475×V1)]÷(V1×Cpr)= 6.8×(△A -0.0302)÷Cpr(2)按(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)鮮(xian)重(zhong)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)NAD+(nmol/g?鮮(xian)重(zhong))= [(△A -0.0302)÷0.1475×V1)]÷(W×V1÷V2)= 13.6×(△A -0.0302)÷W(3)按(an)(an)(an)(an)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)密(mi)度(du)(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)NAD+(nmol/104 cell)=[(△A -0.0302)÷0.1475×V1)]÷(500×V1÷V2)=0.027×(△A -0.0302)(二(er))NADH?含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)標(biao)(biao)準條件(jian)下的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回歸曲(qu)線(xian)(xian)為(wei)(wei)(wei)?y = 0.1404x + 0.0222,R2 = 0.9976;其(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)?NADH?濃度(du)(du)(du)?nmol/mL1、血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)NADH?含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)= [ (△A -0.0222)÷0.1404×V1) ]÷(V3×V1÷V2)= 142.5×(△A -0.0222)2、組(zu)織(zhi)(zhi)(zhi)(zhi)、細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(1)按(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋白(bai)濃度(du)(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)NADH (nmol/mg prot)= [ (△A -0.0222)÷0.1404×V1) ]÷(V1×Cpr)= 7.1×(△A -0.0222)÷Cpr(2)按(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)鮮(xian)重(zhong)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)NADH (nmol/g?鮮(xian)重(zhong))=[(△A -0.0222)÷0.1404×V1) ]÷(W×V1÷V2)= 14.2×(△A -0.0222)÷W(3)按(an)(an)(an)(an)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)密(mi)度(du)(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)NADH (nmol/104 cell)= [ (△A -0.0222)÷0.1404×V1) ]÷(500×V1÷V2)=0.028×(△A -0.0222)V1:加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)反(fan)(fan)應(ying)(ying)體(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji),0.02mL;V2:加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji),2mL;V3:加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)血(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji):0.1mL;Cpr:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋白(bai)質(zhi)(zhi)(zhi)濃度(du)(du)(du),mg/mL; W:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang),g;500:細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)總數(shu)(shu)(shu)(shu),500?萬。注(zhu)(zhu)?意(yi)(yi):?檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian)(xian)為(wei)(wei)(wei)?0.1nmol/mL?或(huo)(huo)(huo)(huo)(huo)?0.1nmol/g?鮮(xian)重(zhong)?或(huo)(huo)(huo)(huo)(huo)?0.001nmol/mg prot
硫氧(yang)還(huan)(huan)蛋白(bai)(bai)氧(yang)化(hua)(hua)(hua)還(huan)(huan)原(yuan)酶(mei)(mei)（TrxR）測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)科(ke)研(yan)(yan)(yan)實(shi)驗(yan),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)臨(lin)床。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)可(ke)(ke)(ke)(ke)以(yi)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)類(lei)型(xing)有(you)(you)(you)哪些(xie)???生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗(yan)原(yuan)理(li)(li)(li)(li)(li)(li)(li)(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)基(ji)于(yu)(yu)(yu)(yu)化(hua)(hua)(hua)學(xue)反(fan)(fan)應,理(li)(li)(li)(li)(li)(li)(li)(li)(li)論上(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)受(shou)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)種(zhong)屬及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)類(lei)型(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)制(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通(tong)常(chang)(chang)(chang)(chang)只(zhi)分(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)類(lei)型(xing),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)種(zhong)屬。但(dan)是(shi)(shi)(shi)(shi)(shi)(shi)某些(xie)指(zhi)標在(zai)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)種(zhong)屬或(huo)(huo)(huo)(huo)(huo)類(lei)型(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方(fang)式(shi)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)。因(yin)此(ci),具體(ti)(ti)(ti)(ti)情況請(qing)參見(jian)(jian)說(shuo)(shuo)明(ming)書(shu)或(huo)(huo)(huo)(huo)(huo)與技術支持聯(lian)系(xi)確認(ren)(ren)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)般(ban)(ban)要(yao)(yao)(yao)(yao)(yao)(yao)求:1)于(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)無溶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)、脂血(xue)(xue)(xue)(xue)(xue)(xue)(xue)、的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現(xian)象。組(zu)(zu)織(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)洗凈擦干(gan)后(hou)(hou)(hou)(hou)(hou)(hou)再進(jin)(jin)(jin)行(xing)(xing)(xing)稱重(zhong)(zhong)處理(li)(li)(li)(li)(li)(li)(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)處理(li)(li)(li)(li)(li)(li)(li)(li)(li)后(hou)(hou)(hou)(hou)(hou)(hou)盡快進(jin)(jin)(jin)行(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且(qie)保(bao)(bao)(bao)存(cun)(cun)(cun)于(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)(bing)(bing)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)之(zhi)(zhi)上(shang)(shang)(shang)(shang),待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。求3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)澄(cheng)(cheng)清(qing)(qing)(qing)(qing)(qing),若不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)澄(cheng)(cheng)清(qing)(qing)(qing)(qing)(qing),需(xu)(xu)(xu)離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)后(hou)(hou)(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)行(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特殊(shu)指(zhi)標除(chu)外)。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)值(zhi)應在(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)線(xian)(xian)性(xing)(xing)(xing)(xing)(xing)(xing)范圍(wei)內(nei)(nei)(nei),若超(chao)過(guo)(guo)(guo)線(xian)(xian)性(xing)(xing)(xing)(xing)(xing)(xing)范圍(wei),需(xu)(xu)(xu)稀釋(shi)后(hou)(hou)(hou)(hou)(hou)(hou)再進(jin)(jin)(jin)行(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若低(di)于(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范圍(wei),需(xu)(xu)(xu)增加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)濃度(du)(du)(du)(du)(du)后(hou)(hou)(hou)(hou)(hou)(hou)進(jin)(jin)(jin)行(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。微(wei)(wei)量(liang)(liang)(liang)(liang)法(fa)(fa)和(he)(he)(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)度(du)(du)(du)(du)(du)法(fa)(fa)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie)(bie)?分(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)度(du)(du)(du)(du)(du)法(fa)(fa):主(zhu)流測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)系(xi)為(wei)(wei)(wei)(wei)(wei)1mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)(yi)器分(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)度(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji),能(neng)(neng)(neng)(neng)(neng)夠大(da)(da)限(xian)度(du)(du)(du)(du)(du)地滿(man)足絕(jue)(jue)大(da)(da)多(duo)數(shu)(shu)(shu)用(yong)(yong)(yong)(yong)戶(hu)(hu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)需(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)。微(wei)(wei)量(liang)(liang)(liang)(liang)法(fa)(fa):主(zhu)流測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)系(xi)為(wei)(wei)(wei)(wei)(wei)0.2mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)(yi)器或(huo)(huo)(huo)(huo)(huo)分(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)度(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji),測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)更(geng)(geng)加(jia)(jia)(jia)簡便快捷(jie)。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)規格(ge)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)是(shi)(shi)(shi)(shi)(shi)(shi)100管(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)(he)(he)50管(guan)(guan)是(shi)(shi)(shi)(shi)(shi)(shi)什么意(yi)(yi)思?(1)50管(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):50管(guan)(guan)指(zhi)能(neng)(neng)(neng)(neng)(neng)夠做(zuo)50次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)可(ke)(ke)(ke)(ke)以(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)48個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)。如(ru)(ru)果(guo)(guo)每個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)重(zhong)(zhong)復(fu)(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci),那么只(zhi)能(neng)(neng)(neng)(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)16個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)。其(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)2次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)空(kong)白(bai)(bai)管(guan)(guan)或(huo)(huo)(huo)(huo)(huo)者(zhe)(zhe)對(dui)(dui)(dui)(dui)照(zhao)管(guan)(guan)。值(zhi)得(de)特別(bie)(bie)注意(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi),50管(guan)(guan)/24樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由于(yu)(yu)(yu)(yu)每個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)需(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)做(zuo)對(dui)(dui)(dui)(dui)照(zhao),因(yin)此(ci)能(neng)(neng)(neng)(neng)(neng)夠測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)僅(jin)為(wei)(wei)(wei)(wei)(wei)8個(ge)(ge)(ge)(假設做(zuo)3次(ci)(ci)重(zhong)(zhong)復(fu)(fu))(2)100管(guan)(guan)/96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):100管(guan)(guan)指(zhi)能(neng)(neng)(neng)(neng)(neng)夠做(zuo)100次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)可(ke)(ke)(ke)(ke)以(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)96個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin),如(ru)(ru)果(guo)(guo)每個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)重(zhong)(zhong)復(fu)(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci),那么只(zhi)能(neng)(neng)(neng)(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)32個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)。其(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)4次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)空(kong)白(bai)(bai)管(guan)(guan)或(huo)(huo)(huo)(huo)(huo)者(zhe)(zhe)對(dui)(dui)(dui)(dui)照(zhao)管(guan)(guan)。注意(yi)(yi)由于(yu)(yu)(yu)(yu)瓶(ping)璧和(he)(he)(he)(he)(he)(he)(he)(he)(he)移液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)器吸(xi)(xi)附等,通(tong)常(chang)(chang)(chang)(chang)要(yao)(yao)(yao)(yao)(yao)(yao)比(bi)(bi)(bi)(bi)理(li)(li)(li)(li)(li)(li)(li)(li)(li)論測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)次(ci)(ci)數(shu)(shu)(shu)少1~3次(ci)(ci)。其(qi)(qi)(qi)(qi)它規格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)(han)(han)(han)義(yi)依次(ci)(ci)類(lei)推(tui)。值(zhi)得(de)特別(bie)(bie)注意(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi),100管(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由于(yu)(yu)(yu)(yu)每個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)需(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)做(zuo)對(dui)(dui)(dui)(dui)照(zhao),因(yin)此(ci)能(neng)(neng)(neng)(neng)(neng)夠測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)僅(jin)為(wei)(wei)(wei)(wei)(wei)16個(ge)(ge)(ge)(假設做(zuo)3次(ci)(ci)重(zhong)(zhong)復(fu)(fu))。如(ru)(ru)何防止(zhi)(zhi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)失(shi)(shi)(shi)(shi)(shi)(shi)效(xiao)?????不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)(yao)(yao)過(guo)(guo)(guo)早訂(ding)購(gou)(gou),以(yi)免(mian)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)失(shi)(shi)(shi)(shi)(shi)(shi)效(xiao).收到(dao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)后(hou)(hou)(hou)(hou)(hou)(hou),檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)查(cha)外包裝是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)正常(chang)(chang)(chang)(chang)。馬(ma)上(shang)(shang)(shang)(shang)打開,按(an)(an)(an)照(zhao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)說(shuo)(shuo)明(ming)書(shu)核(he)對(dui)(dui)(dui)(dui)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)正確,注意(yi)(yi)瓶(ping)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)狀(zhuang)態是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)與說(shuo)(shuo)明(ming)書(shu)一(yi)(yi)(yi)(yi)(yi)致(zhi)。如(ru)(ru)果(guo)(guo)有(you)(you)(you)問題,或(huo)(huo)(huo)(huo)(huo)者(zhe)(zhe)懷疑(yi),馬(ma)上(shang)(shang)(shang)(shang)聯(lian)系(xi)公司(si)銷(xiao)售(shou),進(jin)(jin)(jin)行(xing)(xing)(xing)確認(ren)(ren)。如(ru)(ru)果(guo)(guo)沒有(you)(you)(you)問題,請(qing)務必按(an)(an)(an)照(zhao)說(shuo)(shuo)明(ming)書(shu)進(jin)(jin)(jin)行(xing)(xing)(xing)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)(bao)存(cun)(cun)(cun)。注意(yi)(yi),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji),可(ke)(ke)(ke)(ke)能(neng)(neng)(neng)(neng)(neng)需(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)保(bao)(bao)(bao)存(cun)(cun)(cun)在(zai)常(chang)(chang)(chang)(chang)溫(25°℃)、4℃和(he)(he)(he)(he)(he)(he)(he)(he)(he)-20℃,務必分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)按(an)(an)(an)照(zhao)要(yao)(yao)(yao)(yao)(yao)(yao)求保(bao)(bao)(bao)存(cun)(cun)(cun),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)(yao)(yao)整(zheng)個(ge)(ge)(ge)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)放置(zhi)(zhi)于(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)(bing)(bing)箱。如(ru)(ru)何進(jin)(jin)(jin)行(xing)(xing)(xing)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)?(1)務必在(zai)7個(ge)(ge)(ge)工(gong)作日內(nei)(nei)(nei)進(jin)(jin)(jin)行(xing)(xing)(xing)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),以(yi)確定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)該試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)適合(he)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin),以(yi)免(mian)造成(cheng)(cheng)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)和(he)(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浪費。(2)選擇2~3個(ge)(ge)(ge)預(yu)(yu)期(qi)差異比(bi)(bi)(bi)(bi)較大(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin),嚴(yan)格(ge)按(an)(an)(an)照(zhao)說(shuo)(shuo)明(ming)書(shu)進(jin)(jin)(jin)行(xing)(xing)(xing)操(cao)作,注意(yi)(yi)儀(yi)(yi)器工(gong)作狀(zhuang)態,操(cao)作規范,控(kong)制(zhi)好測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)條件,尤其(qi)(qi)(qi)(qi)是(shi)(shi)(shi)(shi)(shi)(shi)溫度(du)(du)(du)(du)(du)。(3)如(ru)(ru)實(shi)與公司(si)銷(xiao)售(shou)溝通(tong)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)(jie)果(guo)(guo),公司(si)技術人員會確認(ren)(ren)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)(jie)果(guo)(guo)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)正常(chang)(chang)(chang)(chang),是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)需(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)調整(zheng),如(ru)(ru)何進(jin)(jin)(jin)行(xing)(xing)(xing)調整(zheng),從而(er)確保(bao)(bao)(bao)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)(jie)果(guo)(guo)可(ke)(ke)(ke)(ke)靠(kao)。比(bi)(bi)(bi)(bi)色(se)(se)皿有(you)(you)(you)哪些(xie)規格(ge)?(1)常(chang)(chang)(chang)(chang)量(liang)(liang)(liang)(liang)比(bi)(bi)(bi)(bi)色(se)(se)皿,如(ru)(ru)1mL和(he)(he)(he)(he)(he)(he)(he)(he)(he)3mL等,常(chang)(chang)(chang)(chang)量(liang)(liang)(liang)(liang)法(fa)(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)都(dou)是(shi)(shi)(shi)(shi)(shi)(shi)選用(yong)(yong)(yong)(yong)1mL體(ti)(ti)(ti)(ti)系(xi),只(zhi)能(neng)(neng)(neng)(neng)(neng)用(yong)(yong)(yong)(yong)1mL比(bi)(bi)(bi)(bi)色(se)(se)皿,不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)可(ke)(ke)(ke)(ke)以(yi)用(yong)(yong)(yong)(yong)更(geng)(geng)大(da)(da)體(ti)(ti)(ti)(ti)積(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)色(se)(se)皿,否(fou)(fou)則試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)量(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)夠,無法(fa)(fa)正常(chang)(chang)(chang)(chang)比(bi)(bi)(bi)(bi)色(se)(se)。(2)微(wei)(wei)量(liang)(liang)(liang)(liang)比(bi)(bi)(bi)(bi)色(se)(se)皿,如(ru)(ru)0.25mL和(he)(he)(he)(he)(he)(he)(he)(he)(he)0.5mL等,微(wei)(wei)量(liang)(liang)(liang)(liang)法(fa)(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)都(dou)是(shi)(shi)(shi)(shi)(shi)(shi)選用(yong)(yong)(yong)(yong)0.25mL比(bi)(bi)(bi)(bi)色(se)(se)皿。一(yi)(yi)(yi)(yi)(yi)般(ban)(ban)客戶(hu)(hu)都(dou)選擇使(shi)(shi)用(yong)(yong)(yong)(yong)標儀(yi)(yi)(需(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)自(zi)(zi)備(bei)(bei)酶(mei)(mei)標板(ban)(ban))(3)紫(zi)外波(bo)長檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)需(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)石英比(bi)(bi)(bi)(bi)色(se)(se)皿,不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)可(ke)(ke)(ke)(ke)以(yi)用(yong)(yong)(yong)(yong)普通(tong)玻璃比(bi)(bi)(bi)(bi)色(se)(se)皿。(4)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)規格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)色(se)(se)皿,其(qi)(qi)(qi)(qi)外觀大(da)(da)小是(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)(yi)(yi)(yi)致(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de),都(dou)是(shi)(shi)(shi)(shi)(shi)(shi)1cm光(guang)(guang)(guang)(guang)徑(也(ye)有(you)(you)(you)0.5cm光(guang)(guang)(guang)(guang)徑的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)色(se)(se)皿,不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)常(chang)(chang)(chang)(chang)見(jian)(jian),也(ye)可(ke)(ke)(ke)(ke)以(yi)用(yong)(yong)(yong)(yong),但(dan)是(shi)(shi)(shi)(shi)(shi)(shi)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)時(shi)(shi)要(yao)(yao)(yao)(yao)(yao)(yao)注意(yi)(yi)修改計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)公式(shi)),其(qi)(qi)(qi)(qi)內(nei)(nei)(nei)容積(ji)(ji)差異在(zai)于(yu)(yu)(yu)(yu)壁的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)厚度(du)(du)(du)(du)(du)。代(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)優點(1)更(geng)(geng)加(jia)(jia)(jia)省事(shi)兒,完(wan)全不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)必為(wei)(wei)(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)犯難(nan)。用(yong)(yong)(yong)(yong)戶(hu)(hu)只(zhi)要(yao)(yao)(yao)(yao)(yao)(yao)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)供樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)和(he)(he)(he)(he)(he)(he)(he)(he)(he)想(xiang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)指(zhi)標,就不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)再需(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)為(wei)(wei)(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)操(cao)心(xin)(xin)(xin)(xin)(xin)了(le),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)再受(shou)到(dao)操(cao)作技能(neng)(neng)(neng)(neng)(neng)、儀(yi)(yi)器設備(bei)(bei)和(he)(he)(he)(he)(he)(he)(he)(he)(he)時(shi)(shi)間的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)制(zhi),就等著拿到(dao)數(shu)(shu)(shu)據寫論文。(2)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)數(shu)(shu)(shu)據更(geng)(geng)有(you)(you)(you)保(bao)(bao)(bao)障。嚴(yan)格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)管(guan)(guan)理(li)(li)(li)(li)(li)(li)(li)(li)(li)體(ti)(ti)(ti)(ti)系(xi),從而(er)保(bao)(bao)(bao)證(zheng)了(le)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)真實(shi)可(ke)(ke)(ke)(ke)靠(kao)。我(wo)們還(huan)(huan)建立(li)了(le)免(mian)費預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)制(zhi)度(du)(du)(du)(du)(du)。(3)防止(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)損(sun)失(shi)(shi)(shi)(shi)(shi)(shi)無論是(shi)(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)戶(hu)(hu)自(zi)(zi)己(ji)完(wan)成(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)全過(guo)(guo)(guo)程(cheng)(cheng),還(huan)(huan)是(shi)(shi)(shi)(shi)(shi)(shi)購(gou)(gou)買試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)完(wan)成(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),都(dou)可(ke)(ke)(ke)(ke)能(neng)(neng)(neng)(neng)(neng)因(yin)為(wei)(wei)(wei)(wei)(wei)缺(que)乏測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)經驗(yan),導(dao)致(zhi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)失(shi)(shi)(shi)(shi)(shi)(shi)敗(bai),從而(er)損(sun)失(shi)(shi)(shi)(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情況。對(dui)(dui)(dui)(dui)于(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)命科(ke)學(xue)研(yan)(yan)(yan)究而(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)是(shi)(shi)(shi)(shi)(shi)(shi)＊珍貴的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)一(yi)(yi)(yi)(yi)(yi)旦損(sun)失(shi)(shi)(shi)(shi)(shi)(shi),輕則造成(cheng)(cheng)研(yan)(yan)(yan)究進(jin)(jin)(jin)度(du)(du)(du)(du)(du)被(bei)耽擱,重(zhong)(zhong)則造成(cheng)(cheng)研(yan)(yan)(yan)究完(wan)全失(shi)(shi)(shi)(shi)(shi)(shi)敗(bai)!代(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)能(neng)(neng)(neng)(neng)(neng)夠充(chong)分(fen)(fen)(fen)(fen)(fen)保(bao)(bao)(bao)證(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)功,從而(er)防止(zhi)(zhi)了(le)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)損(sun)失(shi)(shi)(shi)(shi)(shi)(shi)。當然,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)功的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti),而(er)且(qie)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)只(zhi)有(you)(you)(you)用(yong)(yong)(yong)(yong)戶(hu)(hu)自(zi)(zi)己(ji)才(cai)能(neng)(neng)(neng)(neng)(neng)控(kong)制(zhi)和(he)(he)(he)(he)(he)(he)(he)(he)(he)保(bao)(bao)(bao)證(zheng)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)本(ben)(ben)(ben)(ben)(ben)(ben)原(yuan)理(li)(li)(li)(li)(li)(li)(li)(li)(li)??生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he),又(you)稱生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he),是(shi)(shi)(shi)(shi)(shi)(shi)利(li)用(yong)(yong)(yong)(yong)特定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)成(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)、動(dong)力學(xue)及(ji)化(hua)(hua)(hua)學(xue)反(fan)(fan)應等技術制(zhi)備(bei)(bei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he),用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)(li)(li)(li)(li)(li)(li)(li)(li)學(xue)、病理(li)(li)(li)(li)(li)(li)(li)(li)(li)學(xue)、藥理(li)(li)(li)(li)(li)(li)(li)(li)(li)學(xue)等領域中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)特定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)分(fen)(fen)(fen)(fen)(fen)子或(huo)(huo)(huo)(huo)(huo)化(hua)(hua)(hua)學(xue)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)包括底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)、酶(mei)(mei)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)、探針等多(duo)個(ge)(ge)(ge)組(zu)(zu)成(cheng)(cheng)部(bu)分(fen)(fen)(fen)(fen)(fen),其(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)＊常(chang)(chang)(chang)(chang)用(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)法(fa)(fa)。底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)法(fa)(fa)是(shi)(shi)(shi)(shi)(shi)(shi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)本(ben)(ben)(ben)(ben)(ben)(ben)原(yuan)理(li)(li)(li)(li)(li)(li)(li)(li)(li)。在(zai)該過(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)與酶(mei)(mei)在(zai)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)膜上(shang)(shang)(shang)(shang)結(jie)(jie)合(he)形(xing)成(cheng)(cheng)基(ji)質(zhi)(zhi)(zhi)酶(mei)(mei),進(jin)(jin)(jin)而(er)形(xing)成(cheng)(cheng)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)復(fu)(fu)合(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)。隨著時(shi)(shi)間的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)推(tui)移,底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)復(fu)(fu)合(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)逐漸分(fen)(fen)(fen)(fen)(fen)解(jie),釋(shi)放出分(fen)(fen)(fen)(fen)(fen)子小的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)子大(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)未(wei)(wei)完(wan)全消化(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)。未(wei)(wei)完(wan)全消化(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)可(ke)(ke)(ke)(ke)以(yi)被(bei)一(yi)(yi)(yi)(yi)(yi)系(xi)列的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)代(dai)(dai)謝(xie)(xie),同(tong)時(shi)(shi)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)、酶(mei)(mei)和(he)(he)(he)(he)(he)(he)(he)(he)(he)代(dai)(dai)謝(xie)(xie)產物(wu)(wu)(wu)(wu)(wu)(wu)(wu)都(dou)會對(dui)(dui)(dui)(dui)復(fu)(fu)合(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)穩定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)產生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)影響(xiang),從而(er)影響(xiang)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)果(guo)(guo)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)可(ke)(ke)(ke)(ke)以(yi)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)類(lei)型(xing)有(you)(you)(you)哪些(xie)?? ?生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗(yan)原(yuan)理(li)(li)(li)(li)(li)(li)(li)(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)基(ji)于(yu)(yu)(yu)(yu)化(hua)(hua)(hua)學(xue)反(fan)(fan)應,理(li)(li)(li)(li)(li)(li)(li)(li)(li)論上(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)受(shou)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)種(zhong)屬及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)類(lei)型(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)制(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通(tong)常(chang)(chang)(chang)(chang)只(zhi)分(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)類(lei)型(xing),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)種(zhong)屬。但(dan)是(shi)(shi)(shi)(shi)(shi)(shi)某些(xie)指(zhi)標在(zai)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)種(zhong)屬或(huo)(huo)(huo)(huo)(huo)類(lei)型(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方(fang)式(shi)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)。因(yin)此(ci),具體(ti)(ti)(ti)(ti)情況請(qing)參見(jian)(jian)說(shuo)(shuo)明(ming)書(shu)或(huo)(huo)(huo)(huo)(huo)與技術支持聯(lian)系(xi)確認(ren)(ren)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)般(ban)(ban)要(yao)(yao)(yao)(yao)(yao)(yao)求:1)于(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)＊-好無溶血(xue)(xue)(xue)(xue)(xue)(xue)(xue)、脂血(xue)(xue)(xue)(xue)(xue)(xue)(xue)、黃-疸(dan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現(xian)象。組(zu)(zu)織(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)將(jiang)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)洗凈擦干(gan)后(hou)(hou)(hou)(hou)(hou)(hou)再進(jin)(jin)(jin)行(xing)(xing)(xing)稱重(zhong)(zhong)處理(li)(li)(li)(li)(li)(li)(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)處理(li)(li)(li)(li)(li)(li)(li)(li)(li)后(hou)(hou)(hou)(hou)(hou)(hou)＊-好盡快進(jin)(jin)(jin)行(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且(qie)保(bao)(bao)(bao)存(cun)(cun)(cun)于(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)(bing)(bing)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)之(zhi)(zhi)上(shang)(shang)(shang)(shang),待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)澄(cheng)(cheng)清(qing)(qing)(qing)(qing)(qing),若不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)澄(cheng)(cheng)清(qing)(qing)(qing)(qing)(qing),需(xu)(xu)(xu)離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)后(hou)(hou)(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)行(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特殊(shu)指(zhi)標除(chu)外)。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)值(zhi)應在(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)線(xian)(xian)性(xing)(xing)(xing)(xing)(xing)(xing)范圍(wei)內(nei)(nei)(nei),若超(chao)過(guo)(guo)(guo)線(xian)(xian)性(xing)(xing)(xing)(xing)(xing)(xing)范圍(wei),需(xu)(xu)(xu)稀釋(shi)后(hou)(hou)(hou)(hou)(hou)(hou)再進(jin)(jin)(jin)行(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若低(di)于(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范圍(wei),需(xu)(xu)(xu)增加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)濃度(du)(du)(du)(du)(du)后(hou)(hou)(hou)(hou)(hou)(hou)進(jin)(jin)(jin)行(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。硫氧(yang)還(huan)(huan)蛋白(bai)(bai)氧(yang)化(hua)(hua)(hua)還(huan)(huan)原(yuan)酶(mei)(mei)（TrxR）測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)科(ke)研(yan)(yan)(yan)實(shi)驗(yan),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)臨(lin)床。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)意(yi)(yi)義(yi):輔(fu)酶(mei)(mei)NAD(H)廣泛存(cun)(cun)(cun)在(zai)于(yu)(yu)(yu)(yu)動(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)、植物(wu)(wu)(wu)(wu)(wu)(wu)(wu)、微(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)(he)(he)培養(yang)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),NAD+是(shi)(shi)(shi)(shi)(shi)(shi)糖(tang)酵解(jie)(EMP)和(he)(he)(he)(he)(he)(he)(he)(he)(he)三(san)(san)羧酸(suan)(suan)(suan)循(xun)環(huan)(TCA)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要(yao)(yao)(yao)(yao)(yao)(yao)氫(qing)受(shou)體(ti)(ti)(ti)(ti),生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?NADH?經呼吸(xi)(xi)電(dian)(dian)子鏈(ETC)傳遞把(ba)電(dian)(dian)子交給氧(yang),在(zai)合(he)成(cheng)(cheng)?ATP?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi),形(xing)成(cheng)(cheng)大(da)(da)量(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?ROS,同(tong)時(shi)(shi)?NADH?再生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)為(wei)(wei)(wei)(wei)(wei)?NAD+。糖(tang)、脂、蛋白(bai)(bai)質(zhi)(zhi)(zhi)三(san)(san)大(da)(da)代(dai)(dai)謝(xie)(xie)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)解(jie)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)氧(yang)化(hua)(hua)(hua)反(fan)(fan)應絕(jue)(jue)大(da)(da)部(bu)分(fen)(fen)(fen)(fen)(fen)通(tong)過(guo)(guo)(guo)這一(yi)(yi)(yi)(yi)(yi)體(ti)(ti)(ti)(ti)系(xi)完(wan)成(cheng)(cheng)。NAD(H)含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH/NAD+比(bi)(bi)(bi)(bi)值(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)高低(di)可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)評價糖(tang)酵解(jie)和(he)(he)(he)(he)(he)(he)(he)(he)(he)?TCA?循(xun)環(huan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)強弱。較高的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)NAD(H)及(ji)?NADH/NAD+比(bi)(bi)(bi)(bi)值(zhi)說(shuo)(shuo)明(ming)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)呼吸(xi)(xi)耗氧(yang)量(liang)(liang)(liang)(liang)較高,處于(yu)(yu)(yu)(yu)過(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)狀(zhuang)態。此(ci)外,NADH/NAD+比(bi)(bi)(bi)(bi)值(zhi)升高也(ye)可(ke)(ke)(ke)(ke)抑制(zhi)糖(tang)酵解(jie)和(he)(he)(he)(he)(he)(he)(he)(he)(he)?TCA?循(xun)環(huan)。另外,NAD+降解(jie)產物(wu)(wu)(wu)(wu)(wu)(wu)(wu)對(dui)(dui)(dui)(dui)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)信號傳導(dao)、代(dai)(dai)謝(xie)(xie)和(he)(he)(he)(he)(he)(he)(he)(he)(he)基(ji)因(yin)表達等具有(you)(you)(you)重(zhong)(zhong)要(yao)(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)調控(kong)作用(yong)(yong)(yong)(yong)。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)原(yuan)理(li)(li)(li)(li)(li)(li)(li)(li)(li):分(fen)(fen)(fen)(fen)(fen)別(bie)(bie)用(yong)(yong)(yong)(yong)酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)和(he)(he)(he)(he)(he)(he)(he)(he)(he)堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH,NADH?通(tong)過(guo)(guo)(guo)?PMS?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)遞氫(qing)作用(yong)(yong)(yong)(yong),還(huan)(huan)原(yuan)氧(yang)化(hua)(hua)(hua)型(xing)噻唑(zuo)藍(MTT)為(wei)(wei)(wei)(wei)(wei)甲瓚(zan),在(zai)?570nm?下檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)吸(xi)(xi)光(guang)(guang)(guang)(guang)值(zhi);而(er)?NAD+可(ke)(ke)(ke)(ke)被(bei)乙(yi)醇脫氫(qing)酶(mei)(mei)還(huan)(huan)原(yuan)為(wei)(wei)(wei)(wei)(wei)?NADH,進(jin)(jin)(jin)一(yi)(yi)(yi)(yi)(yi)步(bu)采用(yong)(yong)(yong)(yong)?MTT?還(huan)(huan)原(yuan)法(fa)(fa)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。需(xu)(xu)(xu)自(zi)(zi)備(bei)(bei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)儀(yi)(yi)器和(he)(he)(he)(he)(he)(he)(he)(he)(he)用(yong)(yong)(yong)(yong)品(pin)(pin)(pin)(pin)(pin):酶(mei)(mei)標儀(yi)(yi)、臺式(shi)離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)機、移液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)器、96?孔板(ban)(ban)、研(yan)(yan)(yan)缽、冰(bing)(bing)(bing)(bing)(bing)(bing)和(he)(he)(he)(he)(he)(he)(he)(he)(he)蒸(zheng)餾水(shui)(shui)(shui)。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)成(cheng)(cheng)和(he)(he)(he)(he)(he)(he)(he)(he)(he)配制(zhi):酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)?50mL×1?瓶(ping),4℃保(bao)(bao)(bao)存(cun)(cun)(cun);堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)?50mL×1?瓶(ping),4℃保(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)?10 mL×1?瓶(ping),4℃保(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)二(er):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)?3 mL×1?瓶(ping),4℃保(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)三(san)(san):粉(fen)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping),-20?℃保(bao)(bao)(bao)存(cun)(cun)(cun),用(yong)(yong)(yong)(yong)時(shi)(shi)加(jia)(jia)(jia)入(ru)(ru)?3mL?蒸(zheng)餾水(shui)(shui)(shui),混(hun)(hun)勻(yun),用(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)存(cun)(cun)(cun)一(yi)(yi)(yi)(yi)(yi)周;試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)四(si):粉(fen)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping),4?℃保(bao)(bao)(bao)存(cun)(cun)(cun),用(yong)(yong)(yong)(yong)時(shi)(shi)加(jia)(jia)(jia)入(ru)(ru)?3mL?蒸(zheng)餾水(shui)(shui)(shui),混(hun)(hun)勻(yun),用(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)存(cun)(cun)(cun)一(yi)(yi)(yi)(yi)(yi)周;試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)五(wu):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)?3.6mL×1?瓶(ping),4?℃保(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)?30mL×1?瓶(ping),4?℃保(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)七:液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)?50mL×1?瓶(ping),4?℃保(bao)(bao)(bao)存(cun)(cun)(cun)。NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):1?血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)照(zhao)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))體(ti)(ti)(ti)(ti)積(ji)(ji)(mL):酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)積(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)例(li)(li)(建議(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1mL?血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)),加(jia)(jia)(jia)入(ru)(ru)?1mL?酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)(yu)?5min(蓋(gai)緊(jin),以(yi)防止(zhi)(zhi)水(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi)(shi)(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)?500μL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)照(zhao)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))體(ti)(ti)(ti)(ti)積(ji)(ji)(mL):堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)積(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)例(li)(li)(建議(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1mL血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)),加(jia)(jia)(jia)入(ru)(ru)?1mL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)(yu)?5min(蓋(gai)緊(jin),以(yi)防止(zhi)(zhi)水(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi)(shi)(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)?500μL?酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。2?組(zu)(zu)織(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)照(zhao)組(zu)(zu)織(zhi)(zhi)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(g):酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)積(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)例(li)(li)(建議(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1g?組(zu)(zu)織(zhi)(zhi),加(jia)(jia)(jia)入(ru)(ru)?1mL酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu)研(yan)(yan)(yan)磨,95℃水(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)(yu)?5min(蓋(gai)緊(jin),以(yi)防止(zhi)(zhi)水(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi)(shi)(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)?500μL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)照(zhao)組(zu)(zu)織(zhi)(zhi)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(g):堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)積(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)例(li)(li)(建議(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1g?組(zu)(zu)織(zhi)(zhi),加(jia)(jia)(jia)入(ru)(ru)?1mL堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu)研(yan)(yan)(yan)磨,95℃水(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)(yu)?5min(蓋(gai)緊(jin),以(yi)防止(zhi)(zhi)水(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi)(shi)(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)?500μL?酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3?細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)菌(jun)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先(xian)收集(ji)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)菌(jun)到(dao)離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)管(guan)(guan)內(nei)(nei)(nei),棄上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),按(an)(an)(an)照(zhao)細(xi)(xi)(xi)菌(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)):酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)積(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)例(li)(li)(建議(yi)?500?萬(wan)細(xi)(xi)(xi)菌(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)加(jia)(jia)(jia)入(ru)(ru)?1mL?酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),超(chao)聲(sheng)波(bo)破(po)碎(冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu),功率20%或(huo)(huo)(huo)(huo)(huo)?200W,超(chao)聲(sheng)?3s,間隔?10s,重(zhong)(zhong)復(fu)(fu)?30?次(ci)(ci)),95℃水(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)(yu)?5min(蓋(gai)緊(jin),以(yi)防止(zhi)(zhi)水(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi)(shi)(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)?500μL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先(xian)收集(ji)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)菌(jun)到(dao)離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)管(guan)(guan)內(nei)(nei)(nei),棄上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),按(an)(an)(an)照(zhao)細(xi)(xi)(xi)菌(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)):堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)積(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)例(li)(li)(建議(yi)?500?萬(wan)細(xi)(xi)(xi)菌(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)加(jia)(jia)(jia)入(ru)(ru)?1mL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)),超(chao)聲(sheng)波(bo)破(po)碎(冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu),功率20%或(huo)(huo)(huo)(huo)(huo)?200W,超(chao)聲(sheng)?3s,間隔?10s,重(zhong)(zhong)復(fu)(fu)?30?次(ci)(ci)),95℃水(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)(yu)?5min(蓋(gai)緊(jin),以(yi)防止(zhi)(zhi)水(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi)(shi)(shi)(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)?500μL?酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)待(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。充(chong)分(fen)(fen)(fen)(fen)(fen)混(hun)(hun)勻(yun),靜(jing)置(zhi)(zhi)?5min?后(hou)(hou)(hou)(hou)(hou)(hou),20000g,25℃離(li)(li)(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)?5min,棄上(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),沉(chen)淀(dian)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)入(ru)(ru):混(hun)(hun)勻(yun),取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?200μL?轉移至微(wei)(wei)量(liang)(liang)(liang)(liang)石英比(bi)(bi)(bi)(bi)色(se)(se)皿或(huo)(huo)(huo)(huo)(huo)?96?孔板(ban)(ban)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),570nm?下讀取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)對(dui)(dui)(dui)(dui)照(zhao)吸(xi)(xi)光(guang)(guang)(guang)(guang)值(zhi)?A1?和(he)(he)(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)吸(xi)(xi)光(guang)(guang)(guang)(guang)值(zhi)?A2,計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)△A=A2-A1。注意(yi)(yi)事(shi)項:1、如(ru)(ru)果(guo)(guo)一(yi)(yi)(yi)(yi)(yi)次(ci)(ci)性(xing)(xing)(xing)(xing)(xing)(xing)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)數(shu)(shu)(shu)較多(duo),可(ke)(ke)(ke)(ke)將(jiang)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)、二(er)、三(san)(san)和(he)(he)(he)(he)(he)(he)(he)(he)(he)四(si)按(an)(an)(an)比(bi)(bi)(bi)(bi)例(li)(li)配成(cheng)(cheng)混(hun)(hun)合(he)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。2、對(dui)(dui)(dui)(dui)照(zhao)管(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)步(bu)驟(zou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie)(bie):對(dui)(dui)(dui)(dui)照(zhao)管(guan)(guan)加(jia)(jia)(jia)完(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)、二(er)、三(san)(san)、四(si)和(he)(he)(he)(he)(he)(he)(he)(he)(he)五(wu)后(hou)(hou)(hou)(hou)(hou)(hou)必須馬(ma)上(shang)(shang)(shang)(shang)加(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu);測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)加(jia)(jia)(jia)完(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)、二(er)、三(san)(san)、四(si)和(he)(he)(he)(he)(he)(he)(he)(he)(he)五(wu)后(hou)(hou)(hou)(hou)(hou)(hou)必須反(fan)(fan)應?20min?后(hou)(hou)(hou)(hou)(hou)(hou)再加(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu)。3、反(fan)(fan)應過(guo)(guo)(guo)程(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)注意(yi)(yi)避(bi)光(guang)(guang)(guang)(guang)。4、若?NAD+測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0302,NADH?測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0222,說(shuo)(shuo)明(ming)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)輔(fu)酶(mei)(mei)含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)較低(di),已低(di)于(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian),可(ke)(ke)(ke)(ke)做(zuo)如(ru)(ru)下調整(zheng):(1)將(jiang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)避(bi)光(guang)(guang)(guang)(guang)靜(jing)置(zhi)(zhi)時(shi)(shi)間?20min?延長到(dao)?60min;(2)在(zai)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)階段(duan)增加(jia)(jia)(jia)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)(liang)(liang),即取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?0.2g?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)或(huo)(huo)(huo)(huo)(huo)?0.2mL?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)加(jia)(jia)(jia)入(ru)(ru)?1mL?提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)。5、由于(yu)(yu)(yu)(yu)每一(yi)(yi)(yi)(yi)(yi)個(ge)(ge)(ge)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)需(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)設一(yi)(yi)(yi)(yi)(yi)個(ge)(ge)(ge)對(dui)(dui)(dui)(dui)照(zhao)管(guan)(guan),本(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)(he)?100?管(guan)(guan)保(bao)(bao)(bao)證(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)?48?個(ge)(ge)(ge)?NAD+或(huo)(huo)(huo)(huo)(huo)?NADH.NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan):(一(yi)(yi)(yi)(yi)(yi)) NAD+含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)標準(zhun)條件下的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回歸曲(qu)線(xian)(xian)為(wei)(wei)(wei)(wei)(wei)?y = 0.1475x + 0.0302,R2 = 0.9978;其(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)(wei)(wei)?NAD+濃度(du)(du)(du)(du)(du)?nmol/mL1、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(nmol/mL)=[(△A -0.0302)÷0.1475×V1) ]÷(V3×V1÷V2)=135.6×(△A -0.0302)2、組(zu)(zu)織(zhi)(zhi)、細(xi)(xi)(xi)菌(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(1)按(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)蛋白(bai)(bai)濃度(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+(nmol/mg prot)=[(△A -0.0302)÷0.1475×V1)]÷(V1×Cpr)= 6.8×(△A -0.0302)÷Cpr(2)按(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)鮮重(zhong)(zhong)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+(nmol/g?鮮重(zhong)(zhong))= [(△A -0.0302)÷0.1475×V1)]÷(W×V1÷V2)= 13.6×(△A -0.0302)÷W(3)按(an)(an)(an)細(xi)(xi)(xi)菌(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)密度(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+(nmol/104 cell)=[(△A -0.0302)÷0.1475×V1)]÷(500×V1÷V2)=0.027×(△A -0.0302)(二(er))NADH?含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)標準(zhun)條件下的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回歸曲(qu)線(xian)(xian)為(wei)(wei)(wei)(wei)(wei)?y = 0.1404x + 0.0222,R2 = 0.9976;其(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)(wei)(wei)?NADH?濃度(du)(du)(du)(du)(du)?nmol/mL1、血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH?含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(nmol/mL)= [ (△A -0.0222)÷0.1404×V1) ]÷(V3×V1÷V2)= 142.5×(△A -0.0222)2、組(zu)(zu)織(zhi)(zhi)、細(xi)(xi)(xi)菌(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)(han)(han)(han)量(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(1)按(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)蛋白(bai)(bai)濃度(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH (nmol/mg prot)= [ (△A -0.0222)÷0.1404×V1) ]÷(V1×Cpr)= 7.1×(△A -0.0222)÷Cpr(2)按(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)鮮重(zhong)(zhong)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH (nmol/g?鮮重(zhong)(zhong))=[(△A -0.0222)÷0.1404×V1) ]÷(W×V1÷V2)= 14.2×(△A -0.0222)÷W(3)按(an)(an)(an)細(xi)(xi)(xi)菌(jun)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)密度(du)(du)(du)(du)(du)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH (nmol/104 cell)= [ (△A -0.0222)÷0.1404×V1) ]÷(500×V1÷V2)=0.028×(△A -0.0222)V1:加(jia)(jia)(jia)入(ru)(ru)反(fan)(fan)應體(ti)(ti)(ti)(ti)系(xi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)體(ti)(ti)(ti)(ti)積(ji)(ji),0.02mL;V2:加(jia)(jia)(jia)入(ru)(ru)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)積(ji)(ji),2mL;V3:加(jia)(jia)(jia)入(ru)(ru)血(xue)(xue)(xue)(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))體(ti)(ti)(ti)(ti)積(ji)(ji):0.1mL;Cpr:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)蛋白(bai)(bai)質(zhi)(zhi)(zhi)濃度(du)(du)(du)(du)(du),mg/mL; W:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang),g;500:細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)菌(jun)總(zong)數(shu)(shu)(shu),500?萬(wan)。注?意(yi)(yi):?檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian)為(wei)(wei)(wei)(wei)(wei)?0.1nmol/mL?或(huo)(huo)(huo)(huo)(huo)?0.1nmol/g?鮮重(zhong)(zhong)?或(huo)(huo)(huo)(huo)(huo)?0.001nmol/mg prot
硫(liu)氧(yang)(yang)還(huan)(huan)(huan)(huan)蛋(dan)白過(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)（TPX）測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)本(ben)(ben)品(pin)(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)科研(yan)(yan)實(shi)驗(yan),不(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)臨(lin)床。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)類(lei)(lei)型(xing)(xing)(xing)有(you)哪(na)些???生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗(yan)原(yuan)理(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)基于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)反(fan)應(ying)(ying)(ying)(ying),理(li)(li)論(lun)(lun)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)受生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)種屬(shu)(shu)(shu)及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)類(lei)(lei)型(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)(xian)制(zhi)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通(tong)(tong)(tong)常(chang)只(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)類(lei)(lei)型(xing)(xing)(xing),不(bu)(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)種屬(shu)(shu)(shu)。但(dan)(dan)是(shi)(shi)(shi)(shi)(shi)(shi)某些指(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)標在(zai)(zai)(zai)(zai)(zai)(zai)不(bu)(bu)(bu)(bu)同(tong)種屬(shu)(shu)(shu)或(huo)(huo)(huo)類(lei)(lei)型(xing)(xing)(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方(fang)式(shi)不(bu)(bu)(bu)(bu)同(tong)。因(yin)(yin)此(ci)(ci),具體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)情況(kuang)(kuang)請(qing)參(can)見說(shuo)(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)或(huo)(huo)(huo)與技術支(zhi)(zhi)持聯系(xi)(xi)確(que)認(ren)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)般(ban)要(yao)(yao)(yao)求:1)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)無(wu)溶(rong)血(xue)(xue)(xue)(xue)、脂血(xue)(xue)(xue)(xue)、的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現象。組(zu)(zu)(zu)(zu)織(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)需將(jiang)(jiang)血(xue)(xue)(xue)(xue)液(ye)(ye)(ye)洗凈擦干后(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)行(xing)(xing)稱(cheng)重(zhong)(zhong)(zhong)處(chu)(chu)理(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)處(chu)(chu)理(li)(li)后(hou)(hou)(hou)(hou)(hou)盡(jin)(jin)快進(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且(qie)(qie)(qie)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)盒(he)(he)(he)(he)(he)之上(shang)(shang)(shang)(shang)(shang)(shang)(shang),待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。求3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)需澄(cheng)(cheng)清(qing)(qing),若不(bu)(bu)(bu)(bu)澄(cheng)(cheng)清(qing)(qing),需離(li)(li)(li)(li)(li)心(xin)后(hou)(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)進(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特(te)殊指(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)標除外)。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)值應(ying)(ying)(ying)(ying)在(zai)(zai)(zai)(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)線(xian)(xian)性(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)圍(wei)(wei)內(nei)(nei),若超(chao)過(guo)線(xian)(xian)性(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)圍(wei)(wei),需稀釋后(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若低(di)(di)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)(fan)圍(wei)(wei),需增(zeng)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)濃(nong)(nong)度(du)(du)后(hou)(hou)(hou)(hou)(hou)進(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。微(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法(fa)和(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)光(guang)光(guang)度(du)(du)法(fa)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie)?分(fen)(fen)(fen)(fen)(fen)(fen)光(guang)光(guang)度(du)(du)法(fa):主流測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)為(wei)(wei)(wei)(wei)1mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)儀器(qi)(qi)分(fen)(fen)(fen)(fen)(fen)(fen)光(guang)光(guang)度(du)(du)計(ji)(ji),能(neng)夠(gou)(gou)大(da)(da)(da)限(xian)(xian)(xian)度(du)(du)地滿足絕大(da)(da)(da)多(duo)數(shu)(shu)(shu)用(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)(hu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)需要(yao)(yao)(yao)。微(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法(fa):主流測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)為(wei)(wei)(wei)(wei)0.2mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)儀器(qi)(qi)或(huo)(huo)(huo)分(fen)(fen)(fen)(fen)(fen)(fen)光(guang)光(guang)度(du)(du)計(ji)(ji),測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)更(geng)加(jia)(jia)(jia)(jia)(jia)(jia)簡便快捷。試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)規格(ge)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)是(shi)(shi)(shi)(shi)(shi)(shi)100管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)50管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)是(shi)(shi)(shi)(shi)(shi)(shi)什(shen)么(me)意(yi)思?(1)50管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):50管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)指(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)能(neng)夠(gou)(gou)做50次(ci)(ci)(ci)(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding),48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)48個(ge)(ge)(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)。如(ru)果(guo)(guo)(guo)每(mei)(mei)(mei)個(ge)(ge)(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)重(zhong)(zhong)(zhong)復(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci)(ci)(ci)(ci)(ci)(ci),那么(me)只(zhi)(zhi)能(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)16個(ge)(ge)(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)。其(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)2次(ci)(ci)(ci)(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)空(kong)白管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)或(huo)(huo)(huo)者(zhe)(zhe)對(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)。值得特(te)別(bie)注意(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi),50管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)/24樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)每(mei)(mei)(mei)個(ge)(ge)(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)(dou)(dou)需要(yao)(yao)(yao)做對(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao),因(yin)(yin)此(ci)(ci)能(neng)夠(gou)(gou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)僅為(wei)(wei)(wei)(wei)8個(ge)(ge)(ge)(ge)(ge)(ge)(假(jia)設(she)(she)做3次(ci)(ci)(ci)(ci)(ci)(ci)(ci)重(zhong)(zhong)(zhong)復(fu))(2)100管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)/96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):100管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)指(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)能(neng)夠(gou)(gou)做100次(ci)(ci)(ci)(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding),96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)96個(ge)(ge)(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin),如(ru)果(guo)(guo)(guo)每(mei)(mei)(mei)個(ge)(ge)(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)重(zhong)(zhong)(zhong)復(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci)(ci)(ci)(ci)(ci)(ci),那么(me)只(zhi)(zhi)能(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)32個(ge)(ge)(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)。其(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)4次(ci)(ci)(ci)(ci)(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)空(kong)白管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)或(huo)(huo)(huo)者(zhe)(zhe)對(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)。注意(yi)由(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)瓶(ping)(ping)(ping)(ping)璧和(he)(he)(he)(he)(he)(he)移(yi)液(ye)(ye)(ye)器(qi)(qi)吸(xi)(xi)(xi)附等(deng)(deng)(deng),通(tong)(tong)(tong)常(chang)要(yao)(yao)(yao)比(bi)(bi)(bi)(bi)(bi)(bi)理(li)(li)論(lun)(lun)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)次(ci)(ci)(ci)(ci)(ci)(ci)(ci)數(shu)(shu)(shu)少(shao)1~3次(ci)(ci)(ci)(ci)(ci)(ci)(ci)。其(qi)(qi)(qi)它規格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)(han)(han)(han)(han)(han)義依次(ci)(ci)(ci)(ci)(ci)(ci)(ci)類(lei)(lei)推(tui)(tui)。值得特(te)別(bie)注意(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi),100管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)每(mei)(mei)(mei)個(ge)(ge)(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)(dou)(dou)需要(yao)(yao)(yao)做對(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao),因(yin)(yin)此(ci)(ci)能(neng)夠(gou)(gou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)僅為(wei)(wei)(wei)(wei)16個(ge)(ge)(ge)(ge)(ge)(ge)(假(jia)設(she)(she)做3次(ci)(ci)(ci)(ci)(ci)(ci)(ci)重(zhong)(zhong)(zhong)復(fu))。如(ru)何防(fang)止(zhi)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)失(shi)(shi)效(xiao)(xiao)?????不(bu)(bu)(bu)(bu)要(yao)(yao)(yao)過(guo)早訂購,以(yi)(yi)(yi)免試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)失(shi)(shi)效(xiao)(xiao).收(shou)(shou)到試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)后(hou)(hou)(hou)(hou)(hou),檢(jian)(jian)(jian)查(cha)外包裝(zhuang)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)正(zheng)常(chang)。馬(ma)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)打開,按(an)(an)(an)(an)照(zhao)(zhao)(zhao)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)說(shuo)(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)核對(dui)(dui)(dui)(dui)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)正(zheng)確(que),注意(yi)瓶(ping)(ping)(ping)(ping)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)狀態(tai)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)與說(shuo)(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)一(yi)(yi)(yi)(yi)致(zhi)。如(ru)果(guo)(guo)(guo)有(you)問題,或(huo)(huo)(huo)者(zhe)(zhe)懷疑,馬(ma)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)聯系(xi)(xi)公(gong)司銷售,進(jin)(jin)(jin)行(xing)(xing)確(que)認(ren)。如(ru)果(guo)(guo)(guo)沒(mei)有(you)問題,請(qing)務(wu)必(bi)按(an)(an)(an)(an)照(zhao)(zhao)(zhao)說(shuo)(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)進(jin)(jin)(jin)行(xing)(xing)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)。注意(yi),不(bu)(bu)(bu)(bu)同(tong)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji),可(ke)(ke)(ke)(ke)能(neng)需要(yao)(yao)(yao)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)在(zai)(zai)(zai)(zai)(zai)(zai)常(chang)溫(wen)(25°℃)、4℃和(he)(he)(he)(he)(he)(he)-20℃,務(wu)必(bi)分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)按(an)(an)(an)(an)照(zhao)(zhao)(zhao)要(yao)(yao)(yao)求保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun),不(bu)(bu)(bu)(bu)要(yao)(yao)(yao)整個(ge)(ge)(ge)(ge)(ge)(ge)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)放(fang)置(zhi)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)箱。如(ru)何進(jin)(jin)(jin)行(xing)(xing)預(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)?(1)務(wu)必(bi)在(zai)(zai)(zai)(zai)(zai)(zai)7個(ge)(ge)(ge)(ge)(ge)(ge)工(gong)作(zuo)(zuo)日內(nei)(nei)進(jin)(jin)(jin)行(xing)(xing)預(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding),以(yi)(yi)(yi)確(que)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)該(gai)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)適(shi)合(he)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin),以(yi)(yi)(yi)免造成(cheng)(cheng)(cheng)(cheng)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)和(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浪費。(2)選擇2~3個(ge)(ge)(ge)(ge)(ge)(ge)預(yu)期(qi)差異比(bi)(bi)(bi)(bi)(bi)(bi)較(jiao)(jiao)大(da)(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin),嚴格(ge)按(an)(an)(an)(an)照(zhao)(zhao)(zhao)說(shuo)(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)進(jin)(jin)(jin)行(xing)(xing)操(cao)作(zuo)(zuo),注意(yi)儀器(qi)(qi)工(gong)作(zuo)(zuo)狀態(tai),操(cao)作(zuo)(zuo)規范(fan)(fan),控制(zhi)好測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)條件,尤其(qi)(qi)(qi)是(shi)(shi)(shi)(shi)(shi)(shi)溫(wen)度(du)(du)。(3)如(ru)實(shi)與公(gong)司銷售溝通(tong)(tong)(tong)預(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)結果(guo)(guo)(guo),公(gong)司技術人員會確(que)認(ren)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)結果(guo)(guo)(guo)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)正(zheng)常(chang),是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)需要(yao)(yao)(yao)調(diao)整,如(ru)何進(jin)(jin)(jin)行(xing)(xing)調(diao)整,從(cong)(cong)而(er)確(que)保(bao)(bao)(bao)(bao)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)結果(guo)(guo)(guo)可(ke)(ke)(ke)(ke)靠(kao)。比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min)有(you)哪(na)些規格(ge)?(1)常(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min),如(ru)1mL和(he)(he)(he)(he)(he)(he)3mL等(deng)(deng)(deng),常(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法(fa)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)都(dou)(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)選用(yong)(yong)(yong)(yong)(yong)(yong)1mL體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi),只(zhi)(zhi)能(neng)用(yong)(yong)(yong)(yong)(yong)(yong)1mL比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min),不(bu)(bu)(bu)(bu)可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong)更(geng)大(da)(da)(da)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min),否(fou)則(ze)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)夠(gou)(gou),無(wu)法(fa)正(zheng)常(chang)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)。(2)微(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min),如(ru)0.25mL和(he)(he)(he)(he)(he)(he)0.5mL等(deng)(deng)(deng),微(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法(fa)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)都(dou)(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)選用(yong)(yong)(yong)(yong)(yong)(yong)0.25mL比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min)。一(yi)(yi)(yi)(yi)般(ban)客戶(hu)(hu)都(dou)(dou)(dou)選擇使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)標儀(需要(yao)(yao)(yao)自備酶(mei)(mei)(mei)(mei)(mei)標板)(3)紫(zi)外波長檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)需要(yao)(yao)(yao)石英比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min),不(bu)(bu)(bu)(bu)可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong)普通(tong)(tong)(tong)玻(bo)璃比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min)。(4)不(bu)(bu)(bu)(bu)同(tong)規格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min),其(qi)(qi)(qi)外觀大(da)(da)(da)小是(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)(yi)(yi)致(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de),都(dou)(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)1cm光(guang)徑(也有(you)0.5cm光(guang)徑的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min),不(bu)(bu)(bu)(bu)常(chang)見,也可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong),但(dan)(dan)是(shi)(shi)(shi)(shi)(shi)(shi)計(ji)(ji)算(suan)(suan)時(shi)(shi)(shi)要(yao)(yao)(yao)注意(yi)修(xiu)改計(ji)(ji)算(suan)(suan)公(gong)式(shi)),其(qi)(qi)(qi)內(nei)(nei)容積(ji)(ji)(ji)(ji)差異在(zai)(zai)(zai)(zai)(zai)(zai)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)壁的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)厚(hou)度(du)(du)。代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)優點(1)更(geng)加(jia)(jia)(jia)(jia)(jia)(jia)省事兒,完(wan)全不(bu)(bu)(bu)(bu)必(bi)為(wei)(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)犯難。用(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)(hu)只(zhi)(zhi)要(yao)(yao)(yao)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)供樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)和(he)(he)(he)(he)(he)(he)想測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)指(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)標,就不(bu)(bu)(bu)(bu)再(zai)需要(yao)(yao)(yao)為(wei)(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)操(cao)心(xin)了,不(bu)(bu)(bu)(bu)再(zai)受到操(cao)作(zuo)(zuo)技能(neng)、儀器(qi)(qi)設(she)(she)備和(he)(he)(he)(he)(he)(he)時(shi)(shi)(shi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)(xian)制(zhi),就等(deng)(deng)(deng)著拿到數(shu)(shu)(shu)據(ju)(ju)寫論(lun)(lun)文。(2)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)數(shu)(shu)(shu)據(ju)(ju)更(geng)有(you)保(bao)(bao)(bao)(bao)障。嚴格(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)理(li)(li)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi),從(cong)(cong)而(er)保(bao)(bao)(bao)(bao)證(zheng)(zheng)了測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)結果(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)真(zhen)實(shi)可(ke)(ke)(ke)(ke)靠(kao)。我們還(huan)(huan)(huan)(huan)建(jian)(jian)(jian)立(li)了免費預(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)制(zhi)度(du)(du)。(3)防(fang)止(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)損(sun)失(shi)(shi)無(wu)論(lun)(lun)是(shi)(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)(hu)自己完(wan)成(cheng)(cheng)(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)全過(guo)程(cheng)(cheng),還(huan)(huan)(huan)(huan)是(shi)(shi)(shi)(shi)(shi)(shi)購買試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)完(wan)成(cheng)(cheng)(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding),都(dou)(dou)(dou)可(ke)(ke)(ke)(ke)能(neng)因(yin)(yin)為(wei)(wei)(wei)(wei)缺乏測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)經驗(yan),導致(zhi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)失(shi)(shi)敗(bai),從(cong)(cong)而(er)損(sun)失(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情況(kuang)(kuang)。對(dui)(dui)(dui)(dui)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)命科學(xue)(xue)(xue)研(yan)(yan)究而(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)是(shi)(shi)(shi)(shi)(shi)(shi)＊珍貴(gui)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)一(yi)(yi)(yi)(yi)旦損(sun)失(shi)(shi),輕(qing)則(ze)造成(cheng)(cheng)(cheng)(cheng)研(yan)(yan)究進(jin)(jin)(jin)度(du)(du)被(bei)耽擱,重(zhong)(zhong)(zhong)則(ze)造成(cheng)(cheng)(cheng)(cheng)研(yan)(yan)究完(wan)全失(shi)(shi)敗(bai)!代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)能(neng)夠(gou)(gou)充(chong)分(fen)(fen)(fen)(fen)(fen)(fen)保(bao)(bao)(bao)(bao)證(zheng)(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)(cheng)(cheng)功(gong),從(cong)(cong)而(er)防(fang)止(zhi)了樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)損(sun)失(shi)(shi)。當(dang)然,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)(cheng)(cheng)功(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti),而(er)且(qie)(qie)(qie)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)只(zhi)(zhi)有(you)用(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)(hu)自己才能(neng)控制(zhi)和(he)(he)(he)(he)(he)(he)保(bao)(bao)(bao)(bao)證(zheng)(zheng)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基本(ben)(ben)原(yuan)理(li)(li)??生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he),又稱(cheng)生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he),是(shi)(shi)(shi)(shi)(shi)(shi)利用(yong)(yong)(yong)(yong)(yong)(yong)特(te)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)、動(dong)力學(xue)(xue)(xue)及(ji)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)反(fan)應(ying)(ying)(ying)(ying)等(deng)(deng)(deng)技術制(zhi)備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he),用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)生(sheng)(sheng)(sheng)理(li)(li)學(xue)(xue)(xue)、病理(li)(li)學(xue)(xue)(xue)、藥理(li)(li)學(xue)(xue)(xue)等(deng)(deng)(deng)領域(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)特(te)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)分(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)或(huo)(huo)(huo)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)物(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)包括(kuo)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)、酶(mei)(mei)(mei)(mei)(mei)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)、探針等(deng)(deng)(deng)多(duo)個(ge)(ge)(ge)(ge)(ge)(ge)組(zu)(zu)(zu)(zu)成(cheng)(cheng)(cheng)(cheng)部分(fen)(fen)(fen)(fen)(fen)(fen),其(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)＊常(chang)用(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)(mei)(mei)法(fa)。底(di)物(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)(mei)(mei)法(fa)是(shi)(shi)(shi)(shi)(shi)(shi)生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基本(ben)(ben)原(yuan)理(li)(li)。在(zai)(zai)(zai)(zai)(zai)(zai)該(gai)過(guo)程(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),底(di)物(wu)(wu)(wu)(wu)(wu)(wu)與酶(mei)(mei)(mei)(mei)(mei)在(zai)(zai)(zai)(zai)(zai)(zai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)膜上(shang)(shang)(shang)(shang)(shang)(shang)(shang)結合(he)形成(cheng)(cheng)(cheng)(cheng)基質(zhi)(zhi)(zhi)酶(mei)(mei)(mei)(mei)(mei),進(jin)(jin)(jin)而(er)形成(cheng)(cheng)(cheng)(cheng)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)(mei)(mei)復(fu)合(he)物(wu)(wu)(wu)(wu)(wu)(wu)。隨著時(shi)(shi)(shi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)推(tui)(tui)移(yi),底(di)物(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)(mei)(mei)復(fu)合(he)物(wu)(wu)(wu)(wu)(wu)(wu)逐漸分(fen)(fen)(fen)(fen)(fen)(fen)解,釋放(fang)出分(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)小的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)大(da)(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)未完(wan)全消(xiao)化(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)。未完(wan)全消(xiao)化(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)被(bei)一(yi)(yi)(yi)(yi)系(xi)(xi)列的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)代(dai)謝(xie),同(tong)時(shi)(shi)(shi)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)、酶(mei)(mei)(mei)(mei)(mei)和(he)(he)(he)(he)(he)(he)代(dai)謝(xie)產物(wu)(wu)(wu)(wu)(wu)(wu)都(dou)(dou)(dou)會對(dui)(dui)(dui)(dui)復(fu)合(he)物(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)穩定(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)產生(sheng)(sheng)(sheng)影響,從(cong)(cong)而(er)影響檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結果(guo)(guo)(guo)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)可(ke)(ke)(ke)(ke)以(yi)(yi)(yi)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)類(lei)(lei)型(xing)(xing)(xing)有(you)哪(na)些?? ?生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)驗(yan)原(yuan)理(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)基于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)反(fan)應(ying)(ying)(ying)(ying),理(li)(li)論(lun)(lun)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)受生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)種屬(shu)(shu)(shu)及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)類(lei)(lei)型(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)(xian)制(zhi)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通(tong)(tong)(tong)常(chang)只(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)類(lei)(lei)型(xing)(xing)(xing),不(bu)(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)種屬(shu)(shu)(shu)。但(dan)(dan)是(shi)(shi)(shi)(shi)(shi)(shi)某些指(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)標在(zai)(zai)(zai)(zai)(zai)(zai)不(bu)(bu)(bu)(bu)同(tong)種屬(shu)(shu)(shu)或(huo)(huo)(huo)類(lei)(lei)型(xing)(xing)(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方(fang)式(shi)不(bu)(bu)(bu)(bu)同(tong)。因(yin)(yin)此(ci)(ci),具體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)情況(kuang)(kuang)請(qing)參(can)見說(shuo)(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)或(huo)(huo)(huo)與技術支(zhi)(zhi)持聯系(xi)(xi)確(que)認(ren)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)般(ban)要(yao)(yao)(yao)求:1)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)＊-好無(wu)溶(rong)血(xue)(xue)(xue)(xue)、脂血(xue)(xue)(xue)(xue)、黃-疸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現象。組(zu)(zu)(zu)(zu)織(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)需將(jiang)(jiang)血(xue)(xue)(xue)(xue)液(ye)(ye)(ye)洗凈擦干后(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)行(xing)(xing)稱(cheng)重(zhong)(zhong)(zhong)處(chu)(chu)理(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)處(chu)(chu)理(li)(li)后(hou)(hou)(hou)(hou)(hou)＊-好盡(jin)(jin)快進(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且(qie)(qie)(qie)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)盒(he)(he)(he)(he)(he)之上(shang)(shang)(shang)(shang)(shang)(shang)(shang),待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)需澄(cheng)(cheng)清(qing)(qing),若不(bu)(bu)(bu)(bu)澄(cheng)(cheng)清(qing)(qing),需離(li)(li)(li)(li)(li)心(xin)后(hou)(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)進(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特(te)殊指(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)標除外)。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)值應(ying)(ying)(ying)(ying)在(zai)(zai)(zai)(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)線(xian)(xian)性(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)圍(wei)(wei)內(nei)(nei),若超(chao)過(guo)線(xian)(xian)性(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)圍(wei)(wei),需稀釋后(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若低(di)(di)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)(fan)圍(wei)(wei),需增(zeng)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)濃(nong)(nong)度(du)(du)后(hou)(hou)(hou)(hou)(hou)進(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。硫(liu)氧(yang)(yang)還(huan)(huan)(huan)(huan)蛋(dan)白過(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)（TPX）測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)本(ben)(ben)品(pin)(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)科研(yan)(yan)實(shi)驗(yan),不(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)臨(lin)床。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)意(yi)義:輔酶(mei)(mei)(mei)(mei)(mei)NAD(H)廣泛存(cun)(cun)(cun)(cun)(cun)(cun)在(zai)(zai)(zai)(zai)(zai)(zai)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)動(dong)物(wu)(wu)(wu)(wu)(wu)(wu)、植物(wu)(wu)(wu)(wu)(wu)(wu)、微(wei)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)培養細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),NAD+是(shi)(shi)(shi)(shi)(shi)(shi)糖(tang)(tang)酵解(EMP)和(he)(he)(he)(he)(he)(he)三羧酸(suan)(suan)(suan)循(xun)環(huan)(TCA)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)(yao)(yao)氫(qing)受體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti),生(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?NADH?經呼(hu)吸(xi)(xi)(xi)電(dian)子(zi)(zi)鏈(lian)(ETC)傳遞把電(dian)子(zi)(zi)交給(gei)氧(yang)(yang),在(zai)(zai)(zai)(zai)(zai)(zai)合(he)成(cheng)(cheng)(cheng)(cheng)?ATP?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi)(shi),形成(cheng)(cheng)(cheng)(cheng)大(da)(da)(da)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?ROS,同(tong)時(shi)(shi)(shi)?NADH?再(zai)生(sheng)(sheng)(sheng)為(wei)(wei)(wei)(wei)?NAD+。糖(tang)(tang)、脂、蛋(dan)白質(zhi)(zhi)(zhi)三大(da)(da)(da)代(dai)謝(xie)物(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)解中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)反(fan)應(ying)(ying)(ying)(ying)絕大(da)(da)(da)部分(fen)(fen)(fen)(fen)(fen)(fen)通(tong)(tong)(tong)過(guo)這一(yi)(yi)(yi)(yi)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)完(wan)成(cheng)(cheng)(cheng)(cheng)。NAD(H)含(han)(han)(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)(he)?NADH/NAD+比(bi)(bi)(bi)(bi)(bi)(bi)值的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)高低(di)(di)可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)評價糖(tang)(tang)酵解和(he)(he)(he)(he)(he)(he)?TCA?循(xun)環(huan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)強弱。較(jiao)(jiao)高的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)NAD(H)及(ji)?NADH/NAD+比(bi)(bi)(bi)(bi)(bi)(bi)值說(shuo)(shuo)明(ming)(ming)(ming)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)呼(hu)吸(xi)(xi)(xi)耗(hao)氧(yang)(yang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)較(jiao)(jiao)高,處(chu)(chu)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)過(guo)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)狀態(tai)。此(ci)(ci)外,NADH/NAD+比(bi)(bi)(bi)(bi)(bi)(bi)值升高也可(ke)(ke)(ke)(ke)抑(yi)制(zhi)糖(tang)(tang)酵解和(he)(he)(he)(he)(he)(he)?TCA?循(xun)環(huan)。另外,NAD+降(jiang)解產物(wu)(wu)(wu)(wu)(wu)(wu)對(dui)(dui)(dui)(dui)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)信號傳導、代(dai)謝(xie)和(he)(he)(he)(he)(he)(he)基因(yin)(yin)表達等(deng)(deng)(deng)具有(you)重(zhong)(zhong)(zhong)要(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)調(diao)控作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)原(yuan)理(li)(li):分(fen)(fen)(fen)(fen)(fen)(fen)別(bie)用(yong)(yong)(yong)(yong)(yong)(yong)酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)和(he)(he)(he)(he)(he)(he)堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)?NADH,NADH?通(tong)(tong)(tong)過(guo)?PMS?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)遞氫(qing)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong),還(huan)(huan)(huan)(huan)原(yuan)氧(yang)(yang)化(hua)(hua)(hua)(hua)(hua)型(xing)(xing)(xing)噻唑藍(MTT)為(wei)(wei)(wei)(wei)甲瓚,在(zai)(zai)(zai)(zai)(zai)(zai)?570nm?下檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)吸(xi)(xi)(xi)光(guang)值;而(er)?NAD+可(ke)(ke)(ke)(ke)被(bei)乙醇脫氫(qing)酶(mei)(mei)(mei)(mei)(mei)還(huan)(huan)(huan)(huan)原(yuan)為(wei)(wei)(wei)(wei)?NADH,進(jin)(jin)(jin)一(yi)(yi)(yi)(yi)步采用(yong)(yong)(yong)(yong)(yong)(yong)?MTT?還(huan)(huan)(huan)(huan)原(yuan)法(fa)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。需自備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)儀器(qi)(qi)和(he)(he)(he)(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)品(pin)(pin)(pin)(pin)(pin):酶(mei)(mei)(mei)(mei)(mei)標儀、臺式(shi)離(li)(li)(li)(li)(li)心(xin)機、移(yi)液(ye)(ye)(ye)器(qi)(qi)、96?孔板、研(yan)(yan)缽、冰(bing)(bing)(bing)和(he)(he)(he)(he)(he)(he)蒸餾(liu)水(shui)(shui)(shui)(shui)。試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)(zu)(zu)成(cheng)(cheng)(cheng)(cheng)和(he)(he)(he)(he)(he)(he)配(pei)制(zhi):酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye):液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping)(ping)(ping),4℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun);堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye):液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping)(ping)(ping),4℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi):液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)?10 mL×1?瓶(ping)(ping)(ping)(ping),4℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)二:液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)?3 mL×1?瓶(ping)(ping)(ping)(ping),4℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)三:粉(fen)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping)(ping)(ping)(ping),-20?℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun),用(yong)(yong)(yong)(yong)(yong)(yong)時(shi)(shi)(shi)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?3mL?蒸餾(liu)水(shui)(shui)(shui)(shui),混(hun)(hun)(hun)勻(yun)(yun),用(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)一(yi)(yi)(yi)(yi)周;試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)四(si):粉(fen)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping)(ping)(ping)(ping),4?℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun),用(yong)(yong)(yong)(yong)(yong)(yong)時(shi)(shi)(shi)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?3mL?蒸餾(liu)水(shui)(shui)(shui)(shui),混(hun)(hun)(hun)勻(yun)(yun),用(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)一(yi)(yi)(yi)(yi)周;試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)五:液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)?3.6mL×1?瓶(ping)(ping)(ping)(ping),4?℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)六:液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)?30mL×1?瓶(ping)(ping)(ping)(ping),4?℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)七:液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping)(ping)(ping),4?℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)。NAD+和(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):1?血(xue)(xue)(xue)(xue)清(qing)(qing)(漿(jiang)(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)照(zhao)(zhao)(zhao)血(xue)(xue)(xue)(xue)清(qing)(qing)(漿(jiang)(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(mL):酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(建(jian)(jian)(jian)議取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1mL?血(xue)(xue)(xue)(xue)清(qing)(qing)(漿(jiang)(jiang)(jiang)),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?1mL?酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋(gai)緊(jin),以(yi)(yi)(yi)防(fang)止(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi));冰(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)液(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)使(shi)之中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he),混(hun)(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing),置(zhi)冰(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)照(zhao)(zhao)(zhao)血(xue)(xue)(xue)(xue)清(qing)(qing)(漿(jiang)(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(mL):堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(建(jian)(jian)(jian)議取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1mL血(xue)(xue)(xue)(xue)清(qing)(qing)(漿(jiang)(jiang)(jiang)),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?1mL?堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋(gai)緊(jin),以(yi)(yi)(yi)防(fang)止(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi));冰(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)液(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?500μL?酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)使(shi)之中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he),混(hun)(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing),置(zhi)冰(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。2?組(zu)(zu)(zu)(zu)織(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)照(zhao)(zhao)(zhao)組(zu)(zu)(zu)(zu)織(zhi)(zhi)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(g):酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(建(jian)(jian)(jian)議取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1g?組(zu)(zu)(zu)(zu)織(zhi)(zhi),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?1mL酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)),冰(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)研(yan)(yan)磨,95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋(gai)緊(jin),以(yi)(yi)(yi)防(fang)止(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi));冰(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)液(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)使(shi)之中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he),混(hun)(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing),置(zhi)冰(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)照(zhao)(zhao)(zhao)組(zu)(zu)(zu)(zu)織(zhi)(zhi)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(g):堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(建(jian)(jian)(jian)議取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1g?組(zu)(zu)(zu)(zu)織(zhi)(zhi),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?1mL堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)),冰(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)研(yan)(yan)磨,95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋(gai)緊(jin),以(yi)(yi)(yi)防(fang)止(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi));冰(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)液(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?500μL?酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)使(shi)之中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he),混(hun)(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing),置(zhi)冰(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3?細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)或(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先收(shou)(shou)集細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)或(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)到離(li)(li)(li)(li)(li)心(xin)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)內(nei)(nei),棄上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing),按(an)(an)(an)(an)照(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)(ge)(ge)(ge)):酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(建(jian)(jian)(jian)議?500?萬細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?1mL?酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)),超(chao)聲波破(po)碎(冰(bing)(bing)(bing)浴(yu)(yu)(yu)(yu),功(gong)率20%或(huo)(huo)(huo)?200W,超(chao)聲?3s,間(jian)隔?10s,重(zhong)(zhong)(zhong)復(fu)?30?次(ci)(ci)(ci)(ci)(ci)(ci)(ci)),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋(gai)緊(jin),以(yi)(yi)(yi)防(fang)止(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi));冰(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)液(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)使(shi)之中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he),混(hun)(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing),置(zhi)冰(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先收(shou)(shou)集細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)或(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)到離(li)(li)(li)(li)(li)心(xin)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)內(nei)(nei),棄上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing),按(an)(an)(an)(an)照(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)(ge)(ge)(ge)):堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(建(jian)(jian)(jian)議?500?萬細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?1mL?堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)),超(chao)聲波破(po)碎(冰(bing)(bing)(bing)浴(yu)(yu)(yu)(yu),功(gong)率20%或(huo)(huo)(huo)?200W,超(chao)聲?3s,間(jian)隔?10s,重(zhong)(zhong)(zhong)復(fu)?30?次(ci)(ci)(ci)(ci)(ci)(ci)(ci)),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋(gai)緊(jin),以(yi)(yi)(yi)防(fang)止(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi));冰(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻(que)(que)后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)液(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?500μL?酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)使(shi)之中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he),混(hun)(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)(li)(li)心(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing),置(zhi)冰(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。充(chong)分(fen)(fen)(fen)(fen)(fen)(fen)混(hun)(hun)(hun)勻(yun)(yun),靜(jing)置(zhi)?5min?后(hou)(hou)(hou)(hou)(hou),20000g,25℃離(li)(li)(li)(li)(li)心(xin)?5min,棄上(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing),沉淀(dian)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru):混(hun)(hun)(hun)勻(yun)(yun),取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?200μL?轉(zhuan)移(yi)至微(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)石英比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min)或(huo)(huo)(huo)?96?孔板中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),570nm?下讀(du)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)對(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)吸(xi)(xi)(xi)光(guang)值?A1?和(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)吸(xi)(xi)(xi)光(guang)值?A2,計(ji)(ji)算(suan)(suan)△A=A2-A1。注意(yi)事項:1、如(ru)果(guo)(guo)(guo)一(yi)(yi)(yi)(yi)次(ci)(ci)(ci)(ci)(ci)(ci)(ci)性(xing)(xing)(xing)(xing)(xing)(xing)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)數(shu)(shu)(shu)較(jiao)(jiao)多(duo),可(ke)(ke)(ke)(ke)將(jiang)(jiang)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)、二、三和(he)(he)(he)(he)(he)(he)四(si)按(an)(an)(an)(an)比(bi)(bi)(bi)(bi)(bi)(bi)例配(pei)成(cheng)(cheng)(cheng)(cheng)混(hun)(hun)(hun)合(he)液(ye)(ye)(ye)。2、對(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)步驟(zou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie):對(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)加(jia)(jia)(jia)(jia)(jia)(jia)完(wan)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)、二、三、四(si)和(he)(he)(he)(he)(he)(he)五后(hou)(hou)(hou)(hou)(hou)必(bi)須馬(ma)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)加(jia)(jia)(jia)(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)六;測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)加(jia)(jia)(jia)(jia)(jia)(jia)完(wan)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)、二、三、四(si)和(he)(he)(he)(he)(he)(he)五后(hou)(hou)(hou)(hou)(hou)必(bi)須反(fan)應(ying)(ying)(ying)(ying)?20min?后(hou)(hou)(hou)(hou)(hou)再(zai)加(jia)(jia)(jia)(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)六。3、反(fan)應(ying)(ying)(ying)(ying)過(guo)程(cheng)(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)注意(yi)避光(guang)。4、若?NAD+測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0302,NADH?測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0222,說(shuo)(shuo)明(ming)(ming)(ming)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)輔酶(mei)(mei)(mei)(mei)(mei)含(han)(han)(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)較(jiao)(jiao)低(di)(di),已低(di)(di)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian)(xian)(xian),可(ke)(ke)(ke)(ke)做如(ru)下調(diao)整:(1)將(jiang)(jiang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)避光(guang)靜(jing)置(zhi)時(shi)(shi)(shi)間(jian)?20min?延長到?60min;(2)在(zai)(zai)(zai)(zai)(zai)(zai)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)階段增(zeng)加(jia)(jia)(jia)(jia)(jia)(jia)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang),即(ji)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?0.2g?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)或(huo)(huo)(huo)?0.2mL?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)?1mL?提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)。5、由(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)(yu)每(mei)(mei)(mei)一(yi)(yi)(yi)(yi)個(ge)(ge)(ge)(ge)(ge)(ge)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)需要(yao)(yao)(yao)設(she)(she)一(yi)(yi)(yi)(yi)個(ge)(ge)(ge)(ge)(ge)(ge)對(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan),本(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)?100?管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)保(bao)(bao)(bao)(bao)證(zheng)(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)?48?個(ge)(ge)(ge)(ge)(ge)(ge)?NAD+或(huo)(huo)(huo)?NADH.NAD+和(he)(he)(he)(he)(he)(he)?NADH?含(han)(han)(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)算(suan)(suan):(一(yi)(yi)(yi)(yi)) NAD+含(han)(han)(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)算(suan)(suan)標準條件下的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回歸曲線(xian)(xian)為(wei)(wei)(wei)(wei)?y = 0.1475x + 0.0302,R2 = 0.9978;其(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)(wei)?NAD+濃(nong)(nong)度(du)(du)?nmol/mL1、血(xue)(xue)(xue)(xue)清(qing)(qing)(漿(jiang)(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)(han)(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)算(suan)(suan)NAD+含(han)(han)(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)=[(△A -0.0302)÷0.1475×V1) ]÷(V3×V1÷V2)=135.6×(△A -0.0302)2、組(zu)(zu)(zu)(zu)織(zhi)(zhi)、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)(han)(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)算(suan)(suan)(1)按(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)蛋(dan)白濃(nong)(nong)度(du)(du)計(ji)(ji)算(suan)(suan)NAD+(nmol/mg prot)=[(△A -0.0302)÷0.1475×V1)]÷(V1×Cpr)= 6.8×(△A -0.0302)÷Cpr(2)按(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)鮮重(zhong)(zhong)(zhong)計(ji)(ji)算(suan)(suan)NAD+(nmol/g?鮮重(zhong)(zhong)(zhong))= [(△A -0.0302)÷0.1475×V1)]÷(W×V1÷V2)= 13.6×(△A -0.0302)÷W(3)按(an)(an)(an)(an)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)密度(du)(du)計(ji)(ji)算(suan)(suan)NAD+(nmol/104 cell)=[(△A -0.0302)÷0.1475×V1)]÷(500×V1÷V2)=0.027×(△A -0.0302)(二)NADH?含(han)(han)(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)算(suan)(suan)標準條件下的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回歸曲線(xian)(xian)為(wei)(wei)(wei)(wei)?y = 0.1404x + 0.0222,R2 = 0.9976;其(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)(wei)?NADH?濃(nong)(nong)度(du)(du)?nmol/mL1、血(xue)(xue)(xue)(xue)清(qing)(qing)(漿(jiang)(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)(han)(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)算(suan)(suan)NADH?含(han)(han)(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)= [ (△A -0.0222)÷0.1404×V1) ]÷(V3×V1÷V2)= 142.5×(△A -0.0222)2、組(zu)(zu)(zu)(zu)織(zhi)(zhi)、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)(han)(han)(han)(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)算(suan)(suan)(1)按(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)蛋(dan)白濃(nong)(nong)度(du)(du)計(ji)(ji)算(suan)(suan)NADH (nmol/mg prot)= [ (△A -0.0222)÷0.1404×V1) ]÷(V1×Cpr)= 7.1×(△A -0.0222)÷Cpr(2)按(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)鮮重(zhong)(zhong)(zhong)計(ji)(ji)算(suan)(suan)NADH (nmol/g?鮮重(zhong)(zhong)(zhong))=[(△A -0.0222)÷0.1404×V1) ]÷(W×V1÷V2)= 14.2×(△A -0.0222)÷W(3)按(an)(an)(an)(an)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)密度(du)(du)計(ji)(ji)算(suan)(suan)NADH (nmol/104 cell)= [ (△A -0.0222)÷0.1404×V1) ]÷(500×V1÷V2)=0.028×(△A -0.0222)V1:加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)反(fan)應(ying)(ying)(ying)(ying)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji),0.02mL;V2:加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji),2mL;V3:加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)血(xue)(xue)(xue)(xue)清(qing)(qing)(漿(jiang)(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji):0.1mL;Cpr:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)蛋(dan)白質(zhi)(zhi)(zhi)濃(nong)(nong)度(du)(du),mg/mL; W:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang),g;500:細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)或(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)總數(shu)(shu)(shu),500?萬。注?意(yi):?檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian)(xian)(xian)為(wei)(wei)(wei)(wei)?0.1nmol/mL?或(huo)(huo)(huo)?0.1nmol/g?鮮重(zhong)(zhong)(zhong)?或(huo)(huo)(huo)?0.001nmol/mg prot
谷胱(guang)(guang)甘(gan)肽(tai)過(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)（GPX）測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)品(pin)(pin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科研實驗,不(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)臨床(chuang)(chuang)。生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)可(ke)(ke)(ke)(ke)以檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing)有(you)(you)哪些(xie)???生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實驗原(yuan)(yuan)理(li)(li)(li)(li)(li)是(shi)(shi)(shi)(shi)基(ji)(ji)(ji)于(yu)(yu)(yu)(yu)(yu)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)反(fan)(fan)應(ying)(ying),理(li)(li)(li)(li)(li)論(lun)(lun)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)不(bu)(bu)(bu)受生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)種屬(shu)及樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限制(zhi)。生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通常(chang)(chang)(chang)(chang)只分(fen)(fen)(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing),不(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)種屬(shu)。但是(shi)(shi)(shi)(shi)某(mou)些(xie)指(zhi)標(biao)在(zai)(zai)不(bu)(bu)(bu)同(tong)(tong)種屬(shu)或(huo)(huo)(huo)(huo)類(lei)(lei)(lei)型(xing)(xing)(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)方式(shi)不(bu)(bu)(bu)同(tong)(tong)。因(yin)此(ci),具體(ti)(ti)(ti)(ti)(ti)(ti)情況(kuang)(kuang)請參(can)見(jian)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)或(huo)(huo)(huo)(huo)與技術(shu)支持(chi)(chi)聯(lian)(lian)系(xi)(xi)確(que)認。生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)般要(yao)(yao)(yao)(yao)(yao)(yao)求:1)于(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)無(wu)溶(rong)血(xue)(xue)(xue)、脂(zhi)血(xue)(xue)(xue)、的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現象。組(zu)(zu)(zu)織(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)將(jiang)血(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)洗凈(jing)擦(ca)干后(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)稱(cheng)重(zhong)(zhong)(zhong)處理(li)(li)(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)處理(li)(li)(li)(li)(li)后(hou)(hou)(hou)(hou)(hou)盡快(kuai)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)于(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)(bing)盒(he)(he)(he)(he)(he)(he)(he)(he)之上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang),待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。求3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)澄(cheng)(cheng)清(qing)(qing)(qing)(qing)(qing),若(ruo)不(bu)(bu)(bu)澄(cheng)(cheng)清(qing)(qing)(qing)(qing)(qing),需(xu)(xu)(xu)(xu)離(li)(li)(li)(li)心(xin)(xin)(xin)后(hou)(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特(te)(te)殊指(zhi)標(biao)除外(wai))。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)值(zhi)應(ying)(ying)在(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)線性(xing)(xing)(xing)(xing)(xing)范(fan)圍(wei)(wei)(wei)內,若(ruo)超(chao)過(guo)線性(xing)(xing)(xing)(xing)(xing)范(fan)圍(wei)(wei)(wei),需(xu)(xu)(xu)(xu)稀釋(shi)后(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若(ruo)低于(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)圍(wei)(wei)(wei),需(xu)(xu)(xu)(xu)增加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)濃度(du)后(hou)(hou)(hou)(hou)(hou)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。微量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法和(he)(he)(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)度(du)法的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie)?分(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)度(du)法:主(zhu)流測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)為(wei)(wei)(wei)(wei)1mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)(yi)(yi)器分(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)度(du)計(ji)(ji),能(neng)(neng)夠(gou)大(da)限度(du)地滿足(zu)絕大(da)多(duo)(duo)數(shu)(shu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)。微量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法:主(zhu)流測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)為(wei)(wei)(wei)(wei)0.2mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)(yi)(yi)器或(huo)(huo)(huo)(huo)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)度(du)計(ji)(ji),測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)更加(jia)(jia)(jia)(jia)(jia)(jia)(jia)簡便快(kuai)捷。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)規(gui)格(ge)(ge)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)是(shi)(shi)(shi)(shi)100管(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)(he)(he)50管(guan)(guan)(guan)是(shi)(shi)(shi)(shi)什么(me)意(yi)(yi)(yi)思?(1)50管(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):50管(guan)(guan)(guan)指(zhi)能(neng)(neng)夠(gou)做(zuo)(zuo)50次(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding),48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)可(ke)(ke)(ke)(ke)以測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)48個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)。如果(guo)(guo)每個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)重(zhong)(zhong)(zhong)復(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci),那(nei)么(me)只能(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)16個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)。其(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)2次(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)空(kong)白(bai)管(guan)(guan)(guan)或(huo)(huo)(huo)(huo)者對(dui)(dui)照(zhao)(zhao)(zhao)管(guan)(guan)(guan)。值(zhi)得特(te)(te)別(bie)注(zhu)意(yi)(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi),50管(guan)(guan)(guan)/24樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由于(yu)(yu)(yu)(yu)(yu)每個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)都需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)做(zuo)(zuo)對(dui)(dui)照(zhao)(zhao)(zhao),因(yin)此(ci)能(neng)(neng)夠(gou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)僅為(wei)(wei)(wei)(wei)8個(ge)(ge)(ge)(假設做(zuo)(zuo)3次(ci)重(zhong)(zhong)(zhong)復(fu))(2)100管(guan)(guan)(guan)/96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):100管(guan)(guan)(guan)指(zhi)能(neng)(neng)夠(gou)做(zuo)(zuo)100次(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding),96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)可(ke)(ke)(ke)(ke)以測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)96個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin),如果(guo)(guo)每個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)重(zhong)(zhong)(zhong)復(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci),那(nei)么(me)只能(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)32個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)。其(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)4次(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)空(kong)白(bai)管(guan)(guan)(guan)或(huo)(huo)(huo)(huo)者對(dui)(dui)照(zhao)(zhao)(zhao)管(guan)(guan)(guan)。注(zhu)意(yi)(yi)(yi)由于(yu)(yu)(yu)(yu)(yu)瓶(ping)(ping)(ping)璧(bi)和(he)(he)(he)(he)(he)(he)(he)(he)(he)移(yi)液(ye)(ye)(ye)(ye)(ye)(ye)器吸附等(deng)(deng),通常(chang)(chang)(chang)(chang)要(yao)(yao)(yao)(yao)(yao)(yao)比(bi)(bi)(bi)(bi)(bi)(bi)理(li)(li)(li)(li)(li)論(lun)(lun)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)次(ci)數(shu)(shu)少1~3次(ci)。其(qi)(qi)(qi)它規(gui)格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含義(yi)依次(ci)類(lei)(lei)(lei)推。值(zhi)得特(te)(te)別(bie)注(zhu)意(yi)(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi),100管(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由于(yu)(yu)(yu)(yu)(yu)每個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)都需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)做(zuo)(zuo)對(dui)(dui)照(zhao)(zhao)(zhao),因(yin)此(ci)能(neng)(neng)夠(gou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)僅為(wei)(wei)(wei)(wei)16個(ge)(ge)(ge)(假設做(zuo)(zuo)3次(ci)重(zhong)(zhong)(zhong)復(fu))。如何(he)(he)防(fang)(fang)(fang)止(zhi)(zhi)(zhi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)失(shi)效(xiao)?????不(bu)(bu)(bu)要(yao)(yao)(yao)(yao)(yao)(yao)過(guo)早訂購(gou),以免(mian)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)失(shi)效(xiao).收到試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)后(hou)(hou)(hou)(hou)(hou),檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)查外(wai)包裝是(shi)(shi)(shi)(shi)否正(zheng)常(chang)(chang)(chang)(chang)。馬上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)打開,按(an)(an)照(zhao)(zhao)(zhao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)核對(dui)(dui)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)數(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)否正(zheng)確(que),注(zhu)意(yi)(yi)(yi)瓶(ping)(ping)(ping)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)狀態(tai)是(shi)(shi)(shi)(shi)否與說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)一(yi)(yi)(yi)致。如果(guo)(guo)有(you)(you)問題,或(huo)(huo)(huo)(huo)者懷疑,馬上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)聯(lian)(lian)系(xi)(xi)公司(si)銷(xiao)售(shou),進(jin)(jin)(jin)(jin)行(xing)(xing)確(que)認。如果(guo)(guo)沒(mei)有(you)(you)問題,請務必(bi)按(an)(an)照(zhao)(zhao)(zhao)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)進(jin)(jin)(jin)(jin)行(xing)(xing)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)。注(zhu)意(yi)(yi)(yi),不(bu)(bu)(bu)同(tong)(tong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji),可(ke)(ke)(ke)(ke)能(neng)(neng)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)在(zai)(zai)常(chang)(chang)(chang)(chang)溫(wen)(25°℃)、4℃和(he)(he)(he)(he)(he)(he)(he)(he)(he)-20℃,務必(bi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)按(an)(an)照(zhao)(zhao)(zhao)要(yao)(yao)(yao)(yao)(yao)(yao)求保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun),不(bu)(bu)(bu)要(yao)(yao)(yao)(yao)(yao)(yao)整(zheng)個(ge)(ge)(ge)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)放置(zhi)于(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)(bing)箱(xiang)。如何(he)(he)進(jin)(jin)(jin)(jin)行(xing)(xing)預(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)?(1)務必(bi)在(zai)(zai)7個(ge)(ge)(ge)工作(zuo)(zuo)(zuo)日內進(jin)(jin)(jin)(jin)行(xing)(xing)預(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding),以確(que)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)該試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)是(shi)(shi)(shi)(shi)否適合(he)(he)(he)您(nin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin),以免(mian)造成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)和(he)(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浪(lang)費(fei)。(2)選(xuan)擇2~3個(ge)(ge)(ge)預(yu)期差(cha)異(yi)(yi)比(bi)(bi)(bi)(bi)(bi)(bi)較(jiao)大(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin),嚴(yan)格(ge)(ge)按(an)(an)照(zhao)(zhao)(zhao)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)進(jin)(jin)(jin)(jin)行(xing)(xing)操(cao)作(zuo)(zuo)(zuo),注(zhu)意(yi)(yi)(yi)儀(yi)(yi)(yi)器工作(zuo)(zuo)(zuo)狀態(tai),操(cao)作(zuo)(zuo)(zuo)規(gui)范(fan),控(kong)(kong)制(zhi)好測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)條(tiao)(tiao)件,尤其(qi)(qi)(qi)是(shi)(shi)(shi)(shi)溫(wen)度(du)。(3)如實與公司(si)銷(xiao)售(shou)溝通預(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)(jie)果(guo)(guo),公司(si)技術(shu)人員會確(que)認測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)(jie)果(guo)(guo)是(shi)(shi)(shi)(shi)否正(zheng)常(chang)(chang)(chang)(chang),是(shi)(shi)(shi)(shi)否需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)調(diao)整(zheng),如何(he)(he)進(jin)(jin)(jin)(jin)行(xing)(xing)調(diao)整(zheng),從(cong)(cong)而(er)(er)確(que)保(bao)(bao)(bao)(bao)您(nin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)(jie)果(guo)(guo)可(ke)(ke)(ke)(ke)靠。比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min)(min)有(you)(you)哪些(xie)規(gui)格(ge)(ge)?(1)常(chang)(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min)(min),如1mL和(he)(he)(he)(he)(he)(he)(he)(he)(he)3mL等(deng)(deng),常(chang)(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)都是(shi)(shi)(shi)(shi)選(xuan)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)1mL體(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi),只能(neng)(neng)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)1mL比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min)(min),不(bu)(bu)(bu)可(ke)(ke)(ke)(ke)以用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)更大(da)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min)(min),否則試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)不(bu)(bu)(bu)夠(gou),無(wu)法正(zheng)常(chang)(chang)(chang)(chang)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)。(2)微量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min)(min),如0.25mL和(he)(he)(he)(he)(he)(he)(he)(he)(he)0.5mL等(deng)(deng),微量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)都是(shi)(shi)(shi)(shi)選(xuan)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)0.25mL比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min)(min)。一(yi)(yi)(yi)般客(ke)戶都選(xuan)擇使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)儀(yi)(yi)(yi)(需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)自(zi)備酶(mei)(mei)(mei)(mei)(mei)標(biao)板)(3)紫(zi)外(wai)波長檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)石英比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min)(min),不(bu)(bu)(bu)可(ke)(ke)(ke)(ke)以用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)普(pu)通玻璃比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min)(min)。(4)不(bu)(bu)(bu)同(tong)(tong)規(gui)格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min)(min),其(qi)(qi)(qi)外(wai)觀大(da)小是(shi)(shi)(shi)(shi)一(yi)(yi)(yi)致的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de),都是(shi)(shi)(shi)(shi)1cm光(guang)(guang)(guang)(guang)(guang)徑(jing)(也有(you)(you)0.5cm光(guang)(guang)(guang)(guang)(guang)徑(jing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min)(min),不(bu)(bu)(bu)常(chang)(chang)(chang)(chang)見(jian),也可(ke)(ke)(ke)(ke)以用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong),但是(shi)(shi)(shi)(shi)計(ji)(ji)算(suan)時要(yao)(yao)(yao)(yao)(yao)(yao)注(zhu)意(yi)(yi)(yi)修改計(ji)(ji)算(suan)公式(shi)),其(qi)(qi)(qi)內容積(ji)(ji)(ji)(ji)(ji)(ji)差(cha)異(yi)(yi)在(zai)(zai)于(yu)(yu)(yu)(yu)(yu)壁的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)厚度(du)。代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)優(you)點(1)更加(jia)(jia)(jia)(jia)(jia)(jia)(jia)省(sheng)事(shi)兒,完(wan)(wan)(wan)全不(bu)(bu)(bu)必(bi)為(wei)(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)犯(fan)難(nan)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶只要(yao)(yao)(yao)(yao)(yao)(yao)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)供樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)和(he)(he)(he)(he)(he)(he)(he)(he)(he)想測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)指(zhi)標(biao),就(jiu)不(bu)(bu)(bu)再(zai)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)為(wei)(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)操(cao)心(xin)(xin)(xin)了,不(bu)(bu)(bu)再(zai)受到操(cao)作(zuo)(zuo)(zuo)技能(neng)(neng)、儀(yi)(yi)(yi)器設備和(he)(he)(he)(he)(he)(he)(he)(he)(he)時間的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限制(zhi),就(jiu)等(deng)(deng)著拿到數(shu)(shu)據(ju)寫(xie)論(lun)(lun)文(wen)。(2)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)數(shu)(shu)據(ju)更有(you)(you)保(bao)(bao)(bao)(bao)障。嚴(yan)格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)管(guan)(guan)(guan)理(li)(li)(li)(li)(li)體(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi),從(cong)(cong)而(er)(er)保(bao)(bao)(bao)(bao)證(zheng)了測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)真實可(ke)(ke)(ke)(ke)靠。我們還(huan)(huan)(huan)建立了免(mian)費(fei)預(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)制(zhi)度(du)。(3)防(fang)(fang)(fang)止(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)損失(shi)無(wu)論(lun)(lun)是(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶自(zi)己(ji)完(wan)(wan)(wan)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)全過(guo)程,還(huan)(huan)(huan)是(shi)(shi)(shi)(shi)購(gou)買試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)完(wan)(wan)(wan)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding),都可(ke)(ke)(ke)(ke)能(neng)(neng)因(yin)為(wei)(wei)(wei)(wei)缺乏測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)經(jing)驗,導致測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)失(shi)敗,從(cong)(cong)而(er)(er)損失(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情況(kuang)(kuang)。對(dui)(dui)于(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)命科學(xue)(xue)研究而(er)(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)是(shi)(shi)(shi)(shi)＊珍貴的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)一(yi)(yi)(yi)旦(dan)損失(shi),輕則造成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)研究進(jin)(jin)(jin)(jin)度(du)被耽擱(ge),重(zhong)(zhong)(zhong)則造成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)研究完(wan)(wan)(wan)全失(shi)敗!代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)能(neng)(neng)夠(gou)充分(fen)(fen)(fen)(fen)(fen)(fen)(fen)保(bao)(bao)(bao)(bao)證(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)功(gong)(gong),從(cong)(cong)而(er)(er)防(fang)(fang)(fang)止(zhi)(zhi)(zhi)了樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)損失(shi)。當(dang)然,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)功(gong)(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前(qian)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti),而(er)(er)且樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)只有(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶自(zi)己(ji)才能(neng)(neng)控(kong)(kong)制(zhi)和(he)(he)(he)(he)(he)(he)(he)(he)(he)保(bao)(bao)(bao)(bao)證(zheng)。生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)(ji)(ji)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)原(yuan)(yuan)理(li)(li)(li)(li)(li)??生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he),又稱(cheng)生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he),是(shi)(shi)(shi)(shi)利用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)特(te)(te)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)、動力學(xue)(xue)及化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)反(fan)(fan)應(ying)(ying)等(deng)(deng)技術(shu)制(zhi)備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he),用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)生(sheng)(sheng)(sheng)(sheng)理(li)(li)(li)(li)(li)學(xue)(xue)、病理(li)(li)(li)(li)(li)學(xue)(xue)、藥理(li)(li)(li)(li)(li)學(xue)(xue)等(deng)(deng)領域中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)特(te)(te)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)或(huo)(huo)(huo)(huo)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)物(wu)(wu)(wu)(wu)質(zhi)(zhi)。生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)包括底(di)(di)物(wu)(wu)(wu)(wu)、酶(mei)(mei)(mei)(mei)(mei)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)、探針等(deng)(deng)多(duo)(duo)個(ge)(ge)(ge)組(zu)(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)部(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen),其(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)＊常(chang)(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)底(di)(di)物(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)(mei)(mei)法。底(di)(di)物(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)(mei)(mei)法是(shi)(shi)(shi)(shi)生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)(ji)(ji)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)原(yuan)(yuan)理(li)(li)(li)(li)(li)。在(zai)(zai)該過(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),底(di)(di)物(wu)(wu)(wu)(wu)與酶(mei)(mei)(mei)(mei)(mei)在(zai)(zai)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)膜上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)結(jie)(jie)合(he)(he)(he)形(xing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)基(ji)(ji)(ji)質(zhi)(zhi)酶(mei)(mei)(mei)(mei)(mei),進(jin)(jin)(jin)(jin)而(er)(er)形(xing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)底(di)(di)物(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)(mei)(mei)復(fu)合(he)(he)(he)物(wu)(wu)(wu)(wu)。隨(sui)著時間的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)推移(yi),底(di)(di)物(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)(mei)(mei)復(fu)合(he)(he)(he)物(wu)(wu)(wu)(wu)逐漸分(fen)(fen)(fen)(fen)(fen)(fen)(fen)解(jie)(jie)(jie),釋(shi)放出分(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)小的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)物(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)(zi)大(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)未完(wan)(wan)(wan)全消化(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)物(wu)(wu)(wu)(wu)。未完(wan)(wan)(wan)全消化(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)物(wu)(wu)(wu)(wu)可(ke)(ke)(ke)(ke)以被一(yi)(yi)(yi)系(xi)(xi)列的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)(mei)(mei)代(dai)謝(xie),同(tong)(tong)時底(di)(di)物(wu)(wu)(wu)(wu)、酶(mei)(mei)(mei)(mei)(mei)和(he)(he)(he)(he)(he)(he)(he)(he)(he)代(dai)謝(xie)產物(wu)(wu)(wu)(wu)都會對(dui)(dui)復(fu)合(he)(he)(he)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)穩定(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)產生(sheng)(sheng)(sheng)(sheng)影響,從(cong)(cong)而(er)(er)影響檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)(jie)果(guo)(guo)。生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)可(ke)(ke)(ke)(ke)以檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing)有(you)(you)哪些(xie)?? ?生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實驗原(yuan)(yuan)理(li)(li)(li)(li)(li)是(shi)(shi)(shi)(shi)基(ji)(ji)(ji)于(yu)(yu)(yu)(yu)(yu)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)反(fan)(fan)應(ying)(ying),理(li)(li)(li)(li)(li)論(lun)(lun)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)不(bu)(bu)(bu)受生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)種屬(shu)及樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限制(zhi)。生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通常(chang)(chang)(chang)(chang)只分(fen)(fen)(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing),不(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)種屬(shu)。但是(shi)(shi)(shi)(shi)某(mou)些(xie)指(zhi)標(biao)在(zai)(zai)不(bu)(bu)(bu)同(tong)(tong)種屬(shu)或(huo)(huo)(huo)(huo)類(lei)(lei)(lei)型(xing)(xing)(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)方式(shi)不(bu)(bu)(bu)同(tong)(tong)。因(yin)此(ci),具體(ti)(ti)(ti)(ti)(ti)(ti)情況(kuang)(kuang)請參(can)見(jian)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)或(huo)(huo)(huo)(huo)與技術(shu)支持(chi)(chi)聯(lian)(lian)系(xi)(xi)確(que)認。生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)般要(yao)(yao)(yao)(yao)(yao)(yao)求:1)于(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)＊-好無(wu)溶(rong)血(xue)(xue)(xue)、脂(zhi)血(xue)(xue)(xue)、黃-疸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現象。組(zu)(zu)(zu)織(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)將(jiang)血(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)洗凈(jing)擦(ca)干后(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)稱(cheng)重(zhong)(zhong)(zhong)處理(li)(li)(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)處理(li)(li)(li)(li)(li)后(hou)(hou)(hou)(hou)(hou)＊-好盡快(kuai)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)于(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)(bing)盒(he)(he)(he)(he)(he)(he)(he)(he)之上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang),待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)澄(cheng)(cheng)清(qing)(qing)(qing)(qing)(qing),若(ruo)不(bu)(bu)(bu)澄(cheng)(cheng)清(qing)(qing)(qing)(qing)(qing),需(xu)(xu)(xu)(xu)離(li)(li)(li)(li)心(xin)(xin)(xin)后(hou)(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特(te)(te)殊指(zhi)標(biao)除外(wai))。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)值(zhi)應(ying)(ying)在(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)線性(xing)(xing)(xing)(xing)(xing)范(fan)圍(wei)(wei)(wei)內,若(ruo)超(chao)過(guo)線性(xing)(xing)(xing)(xing)(xing)范(fan)圍(wei)(wei)(wei),需(xu)(xu)(xu)(xu)稀釋(shi)后(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若(ruo)低于(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)圍(wei)(wei)(wei),需(xu)(xu)(xu)(xu)增加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)濃度(du)后(hou)(hou)(hou)(hou)(hou)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。谷胱(guang)(guang)甘(gan)肽(tai)過(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)物(wu)(wu)(wu)(wu)酶(mei)(mei)(mei)(mei)(mei)（GPX）測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)品(pin)(pin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)科研實驗,不(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)臨床(chuang)(chuang)。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)意(yi)(yi)(yi)義(yi):輔酶(mei)(mei)(mei)(mei)(mei)NAD(H)廣泛存(cun)(cun)(cun)(cun)(cun)(cun)(cun)在(zai)(zai)于(yu)(yu)(yu)(yu)(yu)動物(wu)(wu)(wu)(wu)、植物(wu)(wu)(wu)(wu)、微生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)(he)(he)培養細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),NAD+是(shi)(shi)(shi)(shi)糖(tang)酵解(jie)(jie)(jie)(EMP)和(he)(he)(he)(he)(he)(he)(he)(he)(he)三(san)(san)(san)(san)(san)羧(suo)酸(suan)(suan)(suan)循環(TCA)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要(yao)(yao)(yao)(yao)(yao)(yao)氫受體(ti)(ti)(ti)(ti)(ti)(ti),生(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?NADH?經(jing)呼吸電子(zi)(zi)鏈(ETC)傳(chuan)遞(di)把電子(zi)(zi)交給(gei)氧(yang)(yang)(yang),在(zai)(zai)合(he)(he)(he)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)?ATP?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)(tong)時,形(xing)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)大(da)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?ROS,同(tong)(tong)時?NADH?再(zai)生(sheng)(sheng)(sheng)(sheng)為(wei)(wei)(wei)(wei)?NAD+。糖(tang)、脂(zhi)、蛋(dan)白(bai)質(zhi)(zhi)三(san)(san)(san)(san)(san)大(da)代(dai)謝(xie)物(wu)(wu)(wu)(wu)質(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)解(jie)(jie)(jie)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)氧(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)反(fan)(fan)應(ying)(ying)絕大(da)部(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)通過(guo)這一(yi)(yi)(yi)體(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)完(wan)(wan)(wan)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)。NAD(H)含量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH/NAD+比(bi)(bi)(bi)(bi)(bi)(bi)值(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)高(gao)(gao)低可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)評(ping)價糖(tang)酵解(jie)(jie)(jie)和(he)(he)(he)(he)(he)(he)(he)(he)(he)?TCA?循環的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)強弱(ruo)。較(jiao)高(gao)(gao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)NAD(H)及?NADH/NAD+比(bi)(bi)(bi)(bi)(bi)(bi)值(zhi)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)呼吸耗氧(yang)(yang)(yang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)較(jiao)高(gao)(gao),處于(yu)(yu)(yu)(yu)(yu)過(guo)氧(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)狀態(tai)。此(ci)外(wai),NADH/NAD+比(bi)(bi)(bi)(bi)(bi)(bi)值(zhi)升高(gao)(gao)也可(ke)(ke)(ke)(ke)抑(yi)制(zhi)糖(tang)酵解(jie)(jie)(jie)和(he)(he)(he)(he)(he)(he)(he)(he)(he)?TCA?循環。另外(wai),NAD+降解(jie)(jie)(jie)產物(wu)(wu)(wu)(wu)對(dui)(dui)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)信號傳(chuan)導、代(dai)謝(xie)和(he)(he)(he)(he)(he)(he)(he)(he)(he)基(ji)(ji)(ji)因(yin)表(biao)達等(deng)(deng)具有(you)(you)重(zhong)(zhong)(zhong)要(yao)(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)調(diao)控(kong)(kong)作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)原(yuan)(yuan)理(li)(li)(li)(li)(li):分(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)和(he)(he)(he)(he)(he)(he)(he)(he)(he)堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH,NADH?通過(guo)?PMS?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)遞(di)氫作(zuo)(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong),還(huan)(huan)(huan)原(yuan)(yuan)氧(yang)(yang)(yang)化(hua)(hua)(hua)(hua)(hua)型(xing)(xing)(xing)噻(sai)唑藍(MTT)為(wei)(wei)(wei)(wei)甲瓚,在(zai)(zai)?570nm?下(xia)(xia)(xia)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)吸光(guang)(guang)(guang)(guang)(guang)值(zhi);而(er)(er)?NAD+可(ke)(ke)(ke)(ke)被乙醇脫氫酶(mei)(mei)(mei)(mei)(mei)還(huan)(huan)(huan)原(yuan)(yuan)為(wei)(wei)(wei)(wei)?NADH,進(jin)(jin)(jin)(jin)一(yi)(yi)(yi)步采用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)?MTT?還(huan)(huan)(huan)原(yuan)(yuan)法檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。需(xu)(xu)(xu)(xu)自(zi)備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)儀(yi)(yi)(yi)器和(he)(he)(he)(he)(he)(he)(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)品(pin)(pin):酶(mei)(mei)(mei)(mei)(mei)標(biao)儀(yi)(yi)(yi)、臺(tai)式(shi)離(li)(li)(li)(li)心(xin)(xin)(xin)機、移(yi)液(ye)(ye)(ye)(ye)(ye)(ye)器、96?孔板、研缽(bo)、冰(bing)(bing)(bing)(bing)(bing)和(he)(he)(he)(he)(he)(he)(he)(he)(he)蒸(zheng)餾(liu)(liu)水(shui)(shui)(shui)(shui)。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)(zu)成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)和(he)(he)(he)(he)(he)(he)(he)(he)(he)配制(zhi):酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping)(ping),4℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun);堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping)(ping),4℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi):液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)?10 mL×1?瓶(ping)(ping)(ping),4℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)二(er)(er):液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)?3 mL×1?瓶(ping)(ping)(ping),4℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)三(san)(san)(san)(san)(san):粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping)(ping)(ping),-20?℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun),用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)時加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)?3mL?蒸(zheng)餾(liu)(liu)水(shui)(shui)(shui)(shui),混(hun)(hun)勻,用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)完(wan)(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)一(yi)(yi)(yi)周;試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)四(si):粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping)(ping)(ping),4?℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun),用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)時加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)?3mL?蒸(zheng)餾(liu)(liu)水(shui)(shui)(shui)(shui),混(hun)(hun)勻,用(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)完(wan)(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)一(yi)(yi)(yi)周;試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)五(wu)(wu):液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)?3.6mL×1?瓶(ping)(ping)(ping),4?℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu):液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)?30mL×1?瓶(ping)(ping)(ping),4?℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)七:液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping)(ping),4?℃保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)(cun)。NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu):1?血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(漿(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu):按(an)(an)照(zhao)(zhao)(zhao)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(漿(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(ji)(mL):酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(建議(yi)(yi)(yi)取(qu)(qu)(qu)(qu)(qu)約(yue)(yue)(yue)(yue)?0.1mL?血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(漿(jiang)),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)?1mL?酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋(gai)(gai)緊(jin),以防(fang)(fang)(fang)止(zhi)(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi));冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使(shi)之中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻,10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),置(zhi)冰(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu):按(an)(an)照(zhao)(zhao)(zhao)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(漿(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(ji)(mL):堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(建議(yi)(yi)(yi)取(qu)(qu)(qu)(qu)(qu)約(yue)(yue)(yue)(yue)?0.1mL血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(漿(jiang)),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)?1mL?堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋(gai)(gai)緊(jin),以防(fang)(fang)(fang)止(zhi)(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi));冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)?500μL?酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使(shi)之中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻,10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),置(zhi)冰(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。2?組(zu)(zu)(zu)織(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu):按(an)(an)照(zhao)(zhao)(zhao)組(zu)(zu)(zu)織(zhi)(zhi)(zhi)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(g):酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(建議(yi)(yi)(yi)取(qu)(qu)(qu)(qu)(qu)約(yue)(yue)(yue)(yue)?0.1g?組(zu)(zu)(zu)織(zhi)(zhi)(zhi),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)?1mL酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)研磨(mo),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋(gai)(gai)緊(jin),以防(fang)(fang)(fang)止(zhi)(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi));冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使(shi)之中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻,10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),置(zhi)冰(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu):按(an)(an)照(zhao)(zhao)(zhao)組(zu)(zu)(zu)織(zhi)(zhi)(zhi)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(g):堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(建議(yi)(yi)(yi)取(qu)(qu)(qu)(qu)(qu)約(yue)(yue)(yue)(yue)?0.1g?組(zu)(zu)(zu)織(zhi)(zhi)(zhi),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)?1mL堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)研磨(mo),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋(gai)(gai)緊(jin),以防(fang)(fang)(fang)止(zhi)(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi));冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)?500μL?酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使(shi)之中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻,10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),置(zhi)冰(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3?細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu):先(xian)收集(ji)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)到離(li)(li)(li)(li)心(xin)(xin)(xin)管(guan)(guan)(guan)內,棄(qi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),按(an)(an)照(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)數(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)):酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(建議(yi)(yi)(yi)?500?萬(wan)(wan)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)?1mL?酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),超(chao)聲波破(po)碎(冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu),功(gong)(gong)率(lv)20%或(huo)(huo)(huo)(huo)?200W,超(chao)聲?3s,間隔?10s,重(zhong)(zhong)(zhong)復(fu)?30?次(ci)),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋(gai)(gai)緊(jin),以防(fang)(fang)(fang)止(zhi)(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi));冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使(shi)之中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻,10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),置(zhi)冰(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu):先(xian)收集(ji)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)到離(li)(li)(li)(li)心(xin)(xin)(xin)管(guan)(guan)(guan)內,棄(qi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),按(an)(an)照(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)數(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)):堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(建議(yi)(yi)(yi)?500?萬(wan)(wan)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)?1mL?堿(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),超(chao)聲波破(po)碎(冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu),功(gong)(gong)率(lv)20%或(huo)(huo)(huo)(huo)?200W,超(chao)聲?3s,間隔?10s,重(zhong)(zhong)(zhong)復(fu)?30?次(ci)),95℃水(shui)(shui)(shui)(shui)浴(yu)(yu)(yu)(yu)?5min(蓋(gai)(gai)緊(jin),以防(fang)(fang)(fang)止(zhi)(zhi)(zhi)水(shui)(shui)(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)(san)失(shi));冰(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)(leng)卻后(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)?500μL?酸(suan)(suan)(suan)性(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使(shi)之中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻,10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),置(zhi)冰(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。充分(fen)(fen)(fen)(fen)(fen)(fen)(fen)混(hun)(hun)勻,靜置(zhi)?5min?后(hou)(hou)(hou)(hou)(hou),20000g,25℃離(li)(li)(li)(li)心(xin)(xin)(xin)?5min,棄(qi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing),沉淀中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru):混(hun)(hun)勻,取(qu)(qu)(qu)(qu)(qu)?200μL?轉移(yi)至(zhi)微量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)石英比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)皿(min)(min)(min)(min)或(huo)(huo)(huo)(huo)?96?孔板中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),570nm?下(xia)(xia)(xia)讀取(qu)(qu)(qu)(qu)(qu)對(dui)(dui)照(zhao)(zhao)(zhao)吸光(guang)(guang)(guang)(guang)(guang)值(zhi)?A1?和(he)(he)(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)吸光(guang)(guang)(guang)(guang)(guang)值(zhi)?A2,計(ji)(ji)算(suan)△A=A2-A1。注(zhu)意(yi)(yi)(yi)事(shi)項:1、如果(guo)(guo)一(yi)(yi)(yi)次(ci)性(xing)(xing)(xing)(xing)(xing)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)數(shu)(shu)較(jiao)多(duo)(duo),可(ke)(ke)(ke)(ke)將(jiang)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)、二(er)(er)、三(san)(san)(san)(san)(san)和(he)(he)(he)(he)(he)(he)(he)(he)(he)四(si)按(an)(an)比(bi)(bi)(bi)(bi)(bi)(bi)例配成(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)(cheng)混(hun)(hun)合(he)(he)(he)液(ye)(ye)(ye)(ye)(ye)(ye)。2、對(dui)(dui)照(zhao)(zhao)(zhao)管(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)步驟的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie):對(dui)(dui)照(zhao)(zhao)(zhao)管(guan)(guan)(guan)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)完(wan)(wan)(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)、二(er)(er)、三(san)(san)(san)(san)(san)、四(si)和(he)(he)(he)(he)(he)(he)(he)(he)(he)五(wu)(wu)后(hou)(hou)(hou)(hou)(hou)必(bi)須(xu)馬上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu);測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)完(wan)(wan)(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)、二(er)(er)、三(san)(san)(san)(san)(san)、四(si)和(he)(he)(he)(he)(he)(he)(he)(he)(he)五(wu)(wu)后(hou)(hou)(hou)(hou)(hou)必(bi)須(xu)反(fan)(fan)應(ying)(ying)?20min?后(hou)(hou)(hou)(hou)(hou)再(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu)。3、反(fan)(fan)應(ying)(ying)過(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)注(zhu)意(yi)(yi)(yi)避光(guang)(guang)(guang)(guang)(guang)。4、若(ruo)?NAD+測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0302,NADH?測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0222,說(shuo)(shuo)(shuo)明(ming)(ming)(ming)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)輔酶(mei)(mei)(mei)(mei)(mei)含量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)較(jiao)低,已(yi)低于(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限,可(ke)(ke)(ke)(ke)做(zuo)(zuo)如下(xia)(xia)(xia)調(diao)整(zheng):(1)將(jiang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)避光(guang)(guang)(guang)(guang)(guang)靜置(zhi)時間?20min?延長到?60min;(2)在(zai)(zai)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)階段(duan)增加(jia)(jia)(jia)(jia)(jia)(jia)(jia)取(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang),即取(qu)(qu)(qu)(qu)(qu)?0.2g?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)或(huo)(huo)(huo)(huo)?0.2mL?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)?1mL?提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)。5、由于(yu)(yu)(yu)(yu)(yu)每一(yi)(yi)(yi)個(ge)(ge)(ge)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)(yao)設一(yi)(yi)(yi)個(ge)(ge)(ge)對(dui)(dui)照(zhao)(zhao)(zhao)管(guan)(guan)(guan),本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)?100?管(guan)(guan)(guan)保(bao)(bao)(bao)(bao)證(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)?48?個(ge)(ge)(ge)?NAD+或(huo)(huo)(huo)(huo)?NADH.NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?含量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)算(suan):(一(yi)(yi)(yi)) NAD+含量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)算(suan)標(biao)準條(tiao)(tiao)件下(xia)(xia)(xia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回歸(gui)曲線為(wei)(wei)(wei)(wei)?y = 0.1475x + 0.0302,R2 = 0.9978;其(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)(wei)?NAD+濃度(du)?nmol/mL1、血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(漿(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)算(suan)NAD+含量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)=[(△A -0.0302)÷0.1475×V1) ]÷(V3×V1÷V2)=135.6×(△A -0.0302)2、組(zu)(zu)(zu)織(zhi)(zhi)(zhi)、細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)算(suan)(1)按(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋(dan)白(bai)濃度(du)計(ji)(ji)算(suan)NAD+(nmol/mg prot)=[(△A -0.0302)÷0.1475×V1)]÷(V1×Cpr)= 6.8×(△A -0.0302)÷Cpr(2)按(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)鮮(xian)(xian)重(zhong)(zhong)(zhong)計(ji)(ji)算(suan)NAD+(nmol/g?鮮(xian)(xian)重(zhong)(zhong)(zhong))= [(△A -0.0302)÷0.1475×V1)]÷(W×V1÷V2)= 13.6×(△A -0.0302)÷W(3)按(an)(an)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)密度(du)計(ji)(ji)算(suan)NAD+(nmol/104 cell)=[(△A -0.0302)÷0.1475×V1)]÷(500×V1÷V2)=0.027×(△A -0.0302)(二(er)(er))NADH?含量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)算(suan)標(biao)準條(tiao)(tiao)件下(xia)(xia)(xia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回歸(gui)曲線為(wei)(wei)(wei)(wei)?y = 0.1404x + 0.0222,R2 = 0.9976;其(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)(wei)?NADH?濃度(du)?nmol/mL1、血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(漿(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)算(suan)NADH?含量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)= [ (△A -0.0222)÷0.1404×V1) ]÷(V3×V1÷V2)= 142.5×(△A -0.0222)2、組(zu)(zu)(zu)織(zhi)(zhi)(zhi)、細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)算(suan)(1)按(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋(dan)白(bai)濃度(du)計(ji)(ji)算(suan)NADH (nmol/mg prot)= [ (△A -0.0222)÷0.1404×V1) ]÷(V1×Cpr)= 7.1×(△A -0.0222)÷Cpr(2)按(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)鮮(xian)(xian)重(zhong)(zhong)(zhong)計(ji)(ji)算(suan)NADH (nmol/g?鮮(xian)(xian)重(zhong)(zhong)(zhong))=[(△A -0.0222)÷0.1404×V1) ]÷(W×V1÷V2)= 14.2×(△A -0.0222)÷W(3)按(an)(an)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)密度(du)計(ji)(ji)算(suan)NADH (nmol/104 cell)= [ (△A -0.0222)÷0.1404×V1) ]÷(500×V1÷V2)=0.028×(△A -0.0222)V1:加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)反(fan)(fan)應(ying)(ying)體(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(ji),0.02mL;V2:加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(ji),2mL;V3:加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(漿(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(ji)(ji):0.1mL;Cpr:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋(dan)白(bai)質(zhi)(zhi)濃度(du),mg/mL; W:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang),g;500:細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)總數(shu)(shu),500?萬(wan)(wan)。注(zhu)?意(yi)(yi)(yi):?檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限為(wei)(wei)(wei)(wei)?0.1nmol/mL?或(huo)(huo)(huo)(huo)?0.1nmol/g?鮮(xian)(xian)重(zhong)(zhong)(zhong)?或(huo)(huo)(huo)(huo)?0.001nmol/mg prot
γ-谷氨(an)酰半胱氨(an)酸(suan)連接酶(mei)(mei)(mei)（GCL）測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)科(ke)研(yan)實驗(yan),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)臨(lin)床(chuang)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)可(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)(lei)(lei)型(xing)(xing)(xing)有(you)(you)哪(na)些(xie)???生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實驗(yan)原(yuan)(yuan)(yuan)理(li)(li)(li)(li)(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)基于(yu)(yu)(yu)(yu)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)反(fan)(fan)應(ying)(ying)(ying),理(li)(li)(li)(li)(li)(li)論上(shang)(shang)(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)受(shou)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)種(zhong)(zhong)屬(shu)及樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)(lei)(lei)型(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)制(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通(tong)(tong)常(chang)只(zhi)分(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)(lei)(lei)型(xing)(xing)(xing),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)分(fen)(fen)種(zhong)(zhong)屬(shu)。但(dan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)某些(xie)指(zhi)標(biao)(biao)在(zai)(zai)(zai)(zai)(zai)(zai)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)種(zhong)(zhong)屬(shu)或(huo)(huo)(huo)(huo)(huo)(huo)類(lei)(lei)(lei)(lei)(lei)型(xing)(xing)(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方式不(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)。因此(ci),具(ju)(ju)體(ti)(ti)(ti)(ti)(ti)(ti)情況請(qing)參見說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)或(huo)(huo)(huo)(huo)(huo)(huo)與(yu)(yu)技(ji)(ji)術支(zhi)持聯系(xi)(xi)(xi)確(que)(que)認(ren)(ren)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)般要(yao)(yao)(yao)(yao)(yao)求:1)于(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)(er)(er)(er)(er)(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)無(wu)(wu)溶血(xue)(xue)(xue)(xue)、脂血(xue)(xue)(xue)(xue)、的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現(xian)象(xiang)。組(zu)(zu)織(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)將血(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)洗(xi)(xi)凈擦(ca)干后(hou)(hou)(hou)(hou)再(zai)(zai)進(jin)(jin)行(xing)稱重(zhong)(zhong)(zhong)(zhong)處(chu)(chu)理(li)(li)(li)(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)處(chu)(chu)理(li)(li)(li)(li)(li)(li)后(hou)(hou)(hou)(hou)盡(jin)快(kuai)(kuai)進(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)于(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)(bing)(bing)盒(he)(he)(he)(he)(he)(he)之(zhi)上(shang)(shang)(shang)(shang)(shang)(shang),待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。求3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),若不(bu)(bu)(bu)(bu)(bu)(bu)(bu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),需(xu)(xu)(xu)(xu)離(li)(li)(li)心(xin)(xin)(xin)(xin)后(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特殊指(zhi)標(biao)(biao)除外)。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)值(zhi)應(ying)(ying)(ying)在(zai)(zai)(zai)(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)線性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)圍(wei)內(nei),若超(chao)過(guo)(guo)線性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)圍(wei),需(xu)(xu)(xu)(xu)稀(xi)釋(shi)(shi)后(hou)(hou)(hou)(hou)再(zai)(zai)進(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若低(di)于(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)(fan)圍(wei),需(xu)(xu)(xu)(xu)增(zeng)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)濃(nong)(nong)度(du)(du)(du)后(hou)(hou)(hou)(hou)進(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。微量(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)和(he)(he)(he)(he)分(fen)(fen)光(guang)(guang)光(guang)(guang)度(du)(du)(du)法(fa)(fa)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie)(bie)?分(fen)(fen)光(guang)(guang)光(guang)(guang)度(du)(du)(du)法(fa)(fa):主流測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi)為(wei)(wei)(wei)(wei)(wei)1mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)器(qi)(qi)分(fen)(fen)光(guang)(guang)光(guang)(guang)度(du)(du)(du)計(ji)(ji)(ji),能(neng)(neng)(neng)夠(gou)(gou)(gou)大(da)限(xian)度(du)(du)(du)地滿(man)足(zu)絕(jue)大(da)多(duo)數用(yong)(yong)(yong)(yong)(yong)戶(hu)(hu)(hu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)。微量(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa):主流測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi)為(wei)(wei)(wei)(wei)(wei)0.2mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)器(qi)(qi)或(huo)(huo)(huo)(huo)(huo)(huo)分(fen)(fen)光(guang)(guang)光(guang)(guang)度(du)(du)(du)計(ji)(ji)(ji),測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)更(geng)加(jia)(jia)(jia)(jia)(jia)(jia)簡便快(kuai)(kuai)捷。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)規格(ge)(ge)(ge)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)100管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)和(he)(he)(he)(he)50管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)什么(me)(me)意(yi)(yi)思?(1)50管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):50管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)指(zhi)能(neng)(neng)(neng)夠(gou)(gou)(gou)做(zuo)50次(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)可(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)48個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)。如(ru)(ru)(ru)(ru)果(guo)(guo)(guo)每(mei)(mei)個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)重(zhong)(zhong)(zhong)(zhong)復測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci)(ci),那么(me)(me)只(zhi)能(neng)(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)16個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)。其(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)2次(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)空白(bai)(bai)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)或(huo)(huo)(huo)(huo)(huo)(huo)者對(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)。值(zhi)得特別(bie)(bie)注(zhu)(zhu)意(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi),50管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)/24樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由(you)于(yu)(yu)(yu)(yu)每(mei)(mei)個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)(dou)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)做(zuo)對(dui)(dui)照(zhao)(zhao)(zhao)(zhao),因此(ci)能(neng)(neng)(neng)夠(gou)(gou)(gou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數量(liang)(liang)(liang)(liang)(liang)(liang)僅為(wei)(wei)(wei)(wei)(wei)8個(ge)(ge)(ge)(假(jia)設(she)做(zuo)3次(ci)(ci)(ci)重(zhong)(zhong)(zhong)(zhong)復)(2)100管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)/96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):100管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)指(zhi)能(neng)(neng)(neng)夠(gou)(gou)(gou)做(zuo)100次(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)可(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)96個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin),如(ru)(ru)(ru)(ru)果(guo)(guo)(guo)每(mei)(mei)個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)重(zhong)(zhong)(zhong)(zhong)復測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci)(ci),那么(me)(me)只(zhi)能(neng)(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)32個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)。其(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)4次(ci)(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)空白(bai)(bai)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)或(huo)(huo)(huo)(huo)(huo)(huo)者對(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)。注(zhu)(zhu)意(yi)(yi)由(you)于(yu)(yu)(yu)(yu)瓶(ping)璧和(he)(he)(he)(he)移(yi)(yi)液(ye)(ye)(ye)(ye)(ye)器(qi)(qi)吸(xi)(xi)(xi)附(fu)等,通(tong)(tong)常(chang)要(yao)(yao)(yao)(yao)(yao)比(bi)(bi)(bi)(bi)(bi)(bi)理(li)(li)(li)(li)(li)(li)論測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)次(ci)(ci)(ci)數少1~3次(ci)(ci)(ci)。其(qi)(qi)(qi)(qi)它規格(ge)(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)義依次(ci)(ci)(ci)類(lei)(lei)(lei)(lei)(lei)推(tui)。值(zhi)得特別(bie)(bie)注(zhu)(zhu)意(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi),100管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由(you)于(yu)(yu)(yu)(yu)每(mei)(mei)個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)(dou)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)做(zuo)對(dui)(dui)照(zhao)(zhao)(zhao)(zhao),因此(ci)能(neng)(neng)(neng)夠(gou)(gou)(gou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數量(liang)(liang)(liang)(liang)(liang)(liang)僅為(wei)(wei)(wei)(wei)(wei)16個(ge)(ge)(ge)(假(jia)設(she)做(zuo)3次(ci)(ci)(ci)重(zhong)(zhong)(zhong)(zhong)復)。如(ru)(ru)(ru)(ru)何(he)(he)防(fang)(fang)止(zhi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)失(shi)(shi)(shi)效?????不(bu)(bu)(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)(yao)過(guo)(guo)早訂購,以(yi)(yi)(yi)(yi)(yi)免(mian)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)失(shi)(shi)(shi)效.收到(dao)(dao)(dao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)后(hou)(hou)(hou)(hou),檢(jian)(jian)(jian)(jian)(jian)(jian)查外包裝是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)正(zheng)常(chang)。馬(ma)上(shang)(shang)(shang)(shang)(shang)(shang)打開,按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)核(he)對(dui)(dui)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)數量(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)正(zheng)確(que)(que),注(zhu)(zhu)意(yi)(yi)瓶(ping)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)狀(zhuang)(zhuang)態是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)與(yu)(yu)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)一(yi)(yi)(yi)(yi)致(zhi)。如(ru)(ru)(ru)(ru)果(guo)(guo)(guo)有(you)(you)問(wen)題,或(huo)(huo)(huo)(huo)(huo)(huo)者懷疑,馬(ma)上(shang)(shang)(shang)(shang)(shang)(shang)聯系(xi)(xi)(xi)公(gong)(gong)(gong)司(si)銷售,進(jin)(jin)行(xing)確(que)(que)認(ren)(ren)。如(ru)(ru)(ru)(ru)果(guo)(guo)(guo)沒有(you)(you)問(wen)題,請(qing)務必按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)進(jin)(jin)行(xing)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。注(zhu)(zhu)意(yi)(yi),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji),可(ke)(ke)(ke)能(neng)(neng)(neng)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)分(fen)(fen)別(bie)(bie)保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)在(zai)(zai)(zai)(zai)(zai)(zai)常(chang)溫(wen)(25°℃)、4℃和(he)(he)(he)(he)-20℃,務必分(fen)(fen)別(bie)(bie)按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)要(yao)(yao)(yao)(yao)(yao)求保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)(yao)整(zheng)個(ge)(ge)(ge)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)放置(zhi)(zhi)于(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)(bing)(bing)箱。如(ru)(ru)(ru)(ru)何(he)(he)進(jin)(jin)行(xing)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)?(1)務必在(zai)(zai)(zai)(zai)(zai)(zai)7個(ge)(ge)(ge)工作(zuo)日內(nei)進(jin)(jin)行(xing)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),以(yi)(yi)(yi)(yi)(yi)確(que)(que)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)該試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)適(shi)合(he)(he)您(nin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin),以(yi)(yi)(yi)(yi)(yi)免(mian)造成(cheng)(cheng)(cheng)(cheng)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)和(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浪費。(2)選(xuan)擇(ze)2~3個(ge)(ge)(ge)預(yu)(yu)期(qi)差(cha)異(yi)比(bi)(bi)(bi)(bi)(bi)(bi)較大(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin),嚴(yan)格(ge)(ge)(ge)按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)進(jin)(jin)行(xing)操(cao)作(zuo),注(zhu)(zhu)意(yi)(yi)儀(yi)器(qi)(qi)工作(zuo)狀(zhuang)(zhuang)態,操(cao)作(zuo)規范(fan)(fan),控(kong)制(zhi)好(hao)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)條件(jian),尤其(qi)(qi)(qi)(qi)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)溫(wen)度(du)(du)(du)。(3)如(ru)(ru)(ru)(ru)實與(yu)(yu)公(gong)(gong)(gong)司(si)銷售溝(gou)通(tong)(tong)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結果(guo)(guo)(guo),公(gong)(gong)(gong)司(si)技(ji)(ji)術人(ren)員會確(que)(que)認(ren)(ren)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結果(guo)(guo)(guo)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)正(zheng)常(chang),是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)調整(zheng),如(ru)(ru)(ru)(ru)何(he)(he)進(jin)(jin)行(xing)調整(zheng),從(cong)而(er)(er)(er)(er)(er)(er)確(que)(que)保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)您(nin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結果(guo)(guo)(guo)可(ke)(ke)(ke)靠(kao)。比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)皿(min)(min)(min)有(you)(you)哪(na)些(xie)規格(ge)(ge)(ge)?(1)常(chang)量(liang)(liang)(liang)(liang)(liang)(liang)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)皿(min)(min)(min),如(ru)(ru)(ru)(ru)1mL和(he)(he)(he)(he)3mL等,常(chang)量(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)都(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)選(xuan)用(yong)(yong)(yong)(yong)(yong)1mL體(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi),只(zhi)能(neng)(neng)(neng)用(yong)(yong)(yong)(yong)(yong)1mL比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)皿(min)(min)(min),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)可(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)用(yong)(yong)(yong)(yong)(yong)更(geng)大(da)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)皿(min)(min)(min),否(fou)則試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)量(liang)(liang)(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)夠(gou)(gou)(gou),無(wu)(wu)法(fa)(fa)正(zheng)常(chang)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)。(2)微量(liang)(liang)(liang)(liang)(liang)(liang)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)皿(min)(min)(min),如(ru)(ru)(ru)(ru)0.25mL和(he)(he)(he)(he)0.5mL等,微量(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)都(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)選(xuan)用(yong)(yong)(yong)(yong)(yong)0.25mL比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)皿(min)(min)(min)。一(yi)(yi)(yi)(yi)般客戶(hu)(hu)(hu)都(dou)(dou)選(xuan)擇(ze)使(shi)(shi)用(yong)(yong)(yong)(yong)(yong)標(biao)(biao)儀(yi)(需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)自(zi)(zi)備酶(mei)(mei)(mei)標(biao)(biao)板(ban))(3)紫外波長檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)石(shi)英比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)皿(min)(min)(min),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)可(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)用(yong)(yong)(yong)(yong)(yong)普(pu)通(tong)(tong)玻(bo)璃比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)皿(min)(min)(min)。(4)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)規格(ge)(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)皿(min)(min)(min),其(qi)(qi)(qi)(qi)外觀大(da)小是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)(yi)(yi)致(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de),都(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)1cm光(guang)(guang)徑(也(ye)(ye)有(you)(you)0.5cm光(guang)(guang)徑的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)皿(min)(min)(min),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)常(chang)見,也(ye)(ye)可(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)用(yong)(yong)(yong)(yong)(yong),但(dan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)時(shi)(shi)(shi)要(yao)(yao)(yao)(yao)(yao)注(zhu)(zhu)意(yi)(yi)修改計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)公(gong)(gong)(gong)式),其(qi)(qi)(qi)(qi)內(nei)容(rong)積(ji)(ji)(ji)(ji)差(cha)異(yi)在(zai)(zai)(zai)(zai)(zai)(zai)于(yu)(yu)(yu)(yu)壁的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)厚度(du)(du)(du)。代測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)優點(1)更(geng)加(jia)(jia)(jia)(jia)(jia)(jia)省事兒,完(wan)(wan)(wan)全不(bu)(bu)(bu)(bu)(bu)(bu)(bu)必為(wei)(wei)(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)犯難。用(yong)(yong)(yong)(yong)(yong)戶(hu)(hu)(hu)只(zhi)要(yao)(yao)(yao)(yao)(yao)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)供樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)和(he)(he)(he)(he)想測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)指(zhi)標(biao)(biao),就不(bu)(bu)(bu)(bu)(bu)(bu)(bu)再(zai)(zai)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)為(wei)(wei)(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)操(cao)心(xin)(xin)(xin)(xin)了,不(bu)(bu)(bu)(bu)(bu)(bu)(bu)再(zai)(zai)受(shou)到(dao)(dao)(dao)操(cao)作(zuo)技(ji)(ji)能(neng)(neng)(neng)、儀(yi)器(qi)(qi)設(she)備和(he)(he)(he)(he)時(shi)(shi)(shi)間的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)制(zhi),就等著(zhu)(zhu)拿到(dao)(dao)(dao)數據寫論文。(2)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)數據更(geng)有(you)(you)保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)障。嚴(yan)格(ge)(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)理(li)(li)(li)(li)(li)(li)體(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi),從(cong)而(er)(er)(er)(er)(er)(er)保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)證了測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結果(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)真(zhen)實可(ke)(ke)(ke)靠(kao)。我們還(huan)建立(li)了免(mian)費預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)制(zhi)度(du)(du)(du)。(3)防(fang)(fang)止(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)損失(shi)(shi)(shi)無(wu)(wu)論是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)戶(hu)(hu)(hu)自(zi)(zi)己(ji)完(wan)(wan)(wan)成(cheng)(cheng)(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)全過(guo)(guo)程(cheng),還(huan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)購買試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)完(wan)(wan)(wan)成(cheng)(cheng)(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),都(dou)(dou)可(ke)(ke)(ke)能(neng)(neng)(neng)因為(wei)(wei)(wei)(wei)(wei)缺乏測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)經驗(yan),導(dao)致(zhi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)失(shi)(shi)(shi)敗,從(cong)而(er)(er)(er)(er)(er)(er)損失(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情況。對(dui)(dui)于(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)命(ming)科(ke)學(xue)(xue)(xue)研(yan)究而(er)(er)(er)(er)(er)(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)＊珍貴的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)一(yi)(yi)(yi)(yi)旦損失(shi)(shi)(shi),輕(qing)則造成(cheng)(cheng)(cheng)(cheng)研(yan)究進(jin)(jin)度(du)(du)(du)被(bei)耽(dan)擱,重(zhong)(zhong)(zhong)(zhong)則造成(cheng)(cheng)(cheng)(cheng)研(yan)究完(wan)(wan)(wan)全失(shi)(shi)(shi)敗!代測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)能(neng)(neng)(neng)夠(gou)(gou)(gou)充分(fen)(fen)保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)證測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)(cheng)(cheng)功(gong),從(cong)而(er)(er)(er)(er)(er)(er)防(fang)(fang)止(zhi)了樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)損失(shi)(shi)(shi)。當然,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)(cheng)(cheng)功(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti),而(er)(er)(er)(er)(er)(er)且樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)只(zhi)有(you)(you)用(yong)(yong)(yong)(yong)(yong)戶(hu)(hu)(hu)自(zi)(zi)己(ji)才(cai)能(neng)(neng)(neng)控(kong)制(zhi)和(he)(he)(he)(he)保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)證。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)原(yuan)(yuan)(yuan)理(li)(li)(li)(li)(li)(li)??生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he),又稱生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he),是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)利用(yong)(yong)(yong)(yong)(yong)特定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)成(cheng)(cheng)(cheng)(cheng)分(fen)(fen)、動(dong)力學(xue)(xue)(xue)及化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)反(fan)(fan)應(ying)(ying)(ying)等技(ji)(ji)術制(zhi)備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he),用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)(li)(li)(li)(li)(li)學(xue)(xue)(xue)、病理(li)(li)(li)(li)(li)(li)學(xue)(xue)(xue)、藥理(li)(li)(li)(li)(li)(li)學(xue)(xue)(xue)等領域中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)特定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)分(fen)(fen)子或(huo)(huo)(huo)(huo)(huo)(huo)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)物(wu)質(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)包括底(di)(di)(di)(di)(di)物(wu)、酶(mei)(mei)(mei)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)、探針等多(duo)個(ge)(ge)(ge)組(zu)(zu)成(cheng)(cheng)(cheng)(cheng)部分(fen)(fen),其(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)＊常(chang)用(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)底(di)(di)(di)(di)(di)物(wu)-酶(mei)(mei)(mei)法(fa)(fa)。底(di)(di)(di)(di)(di)物(wu)-酶(mei)(mei)(mei)法(fa)(fa)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)原(yuan)(yuan)(yuan)理(li)(li)(li)(li)(li)(li)。在(zai)(zai)(zai)(zai)(zai)(zai)該過(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),底(di)(di)(di)(di)(di)物(wu)與(yu)(yu)酶(mei)(mei)(mei)在(zai)(zai)(zai)(zai)(zai)(zai)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)膜上(shang)(shang)(shang)(shang)(shang)(shang)結合(he)(he)形(xing)成(cheng)(cheng)(cheng)(cheng)基質(zhi)(zhi)(zhi)酶(mei)(mei)(mei),進(jin)(jin)而(er)(er)(er)(er)(er)(er)形(xing)成(cheng)(cheng)(cheng)(cheng)底(di)(di)(di)(di)(di)物(wu)-酶(mei)(mei)(mei)復合(he)(he)物(wu)。隨(sui)著(zhu)(zhu)時(shi)(shi)(shi)間的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)推(tui)移(yi)(yi),底(di)(di)(di)(di)(di)物(wu)-酶(mei)(mei)(mei)復合(he)(he)物(wu)逐漸分(fen)(fen)解(jie)(jie),釋(shi)(shi)放出分(fen)(fen)子小的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)(di)(di)(di)物(wu)和(he)(he)(he)(he)分(fen)(fen)子大(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)未(wei)完(wan)(wan)(wan)全消化(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)(di)(di)(di)物(wu)。未(wei)完(wan)(wan)(wan)全消化(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)(di)(di)(di)物(wu)可(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)被(bei)一(yi)(yi)(yi)(yi)系(xi)(xi)(xi)列的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)代謝(xie),同(tong)時(shi)(shi)(shi)底(di)(di)(di)(di)(di)物(wu)、酶(mei)(mei)(mei)和(he)(he)(he)(he)代謝(xie)產物(wu)都(dou)(dou)會對(dui)(dui)復合(he)(he)物(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)穩定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)產生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)影(ying)響,從(cong)而(er)(er)(er)(er)(er)(er)影(ying)響檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結果(guo)(guo)(guo)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)可(ke)(ke)(ke)以(yi)(yi)(yi)(yi)(yi)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)(lei)(lei)型(xing)(xing)(xing)有(you)(you)哪(na)些(xie)?? ?生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實驗(yan)原(yuan)(yuan)(yuan)理(li)(li)(li)(li)(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)基于(yu)(yu)(yu)(yu)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)反(fan)(fan)應(ying)(ying)(ying),理(li)(li)(li)(li)(li)(li)論上(shang)(shang)(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)受(shou)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)種(zhong)(zhong)屬(shu)及樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)(lei)(lei)型(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)制(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通(tong)(tong)常(chang)只(zhi)分(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)(lei)(lei)型(xing)(xing)(xing),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)分(fen)(fen)種(zhong)(zhong)屬(shu)。但(dan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)某些(xie)指(zhi)標(biao)(biao)在(zai)(zai)(zai)(zai)(zai)(zai)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)種(zhong)(zhong)屬(shu)或(huo)(huo)(huo)(huo)(huo)(huo)類(lei)(lei)(lei)(lei)(lei)型(xing)(xing)(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方式不(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)。因此(ci),具(ju)(ju)體(ti)(ti)(ti)(ti)(ti)(ti)情況請(qing)參見說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)或(huo)(huo)(huo)(huo)(huo)(huo)與(yu)(yu)技(ji)(ji)術支(zhi)持聯系(xi)(xi)(xi)確(que)(que)認(ren)(ren)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)般要(yao)(yao)(yao)(yao)(yao)求:1)于(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)(er)(er)(er)(er)(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)＊-好(hao)無(wu)(wu)溶血(xue)(xue)(xue)(xue)、脂血(xue)(xue)(xue)(xue)、黃-疸的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現(xian)象(xiang)。組(zu)(zu)織(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)將血(xue)(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)洗(xi)(xi)凈擦(ca)干后(hou)(hou)(hou)(hou)再(zai)(zai)進(jin)(jin)行(xing)稱重(zhong)(zhong)(zhong)(zhong)處(chu)(chu)理(li)(li)(li)(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)處(chu)(chu)理(li)(li)(li)(li)(li)(li)后(hou)(hou)(hou)(hou)＊-好(hao)盡(jin)快(kuai)(kuai)進(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)于(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)(bing)(bing)盒(he)(he)(he)(he)(he)(he)之(zhi)上(shang)(shang)(shang)(shang)(shang)(shang),待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),若不(bu)(bu)(bu)(bu)(bu)(bu)(bu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),需(xu)(xu)(xu)(xu)離(li)(li)(li)心(xin)(xin)(xin)(xin)后(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特殊指(zhi)標(biao)(biao)除外)。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)值(zhi)應(ying)(ying)(ying)在(zai)(zai)(zai)(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)線性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)圍(wei)內(nei),若超(chao)過(guo)(guo)線性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)圍(wei),需(xu)(xu)(xu)(xu)稀(xi)釋(shi)(shi)后(hou)(hou)(hou)(hou)再(zai)(zai)進(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若低(di)于(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)(fan)圍(wei),需(xu)(xu)(xu)(xu)增(zeng)加(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)濃(nong)(nong)度(du)(du)(du)后(hou)(hou)(hou)(hou)進(jin)(jin)行(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。γ-谷氨(an)酰半胱氨(an)酸(suan)連接酶(mei)(mei)(mei)（GCL）測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)科(ke)研(yan)實驗(yan),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)臨(lin)床(chuang)。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)意(yi)(yi)義:輔酶(mei)(mei)(mei)NAD(H)廣泛存(cun)(cun)(cun)在(zai)(zai)(zai)(zai)(zai)(zai)于(yu)(yu)(yu)(yu)動(dong)物(wu)、植(zhi)物(wu)、微生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)和(he)(he)(he)(he)培養細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),NAD+是(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)糖酵(jiao)解(jie)(jie)(EMP)和(he)(he)(he)(he)三(san)羧酸(suan)循(xun)(xun)環(huan)(TCA)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)(yao)(yao)(yao)(yao)氫受(shou)體(ti)(ti)(ti)(ti)(ti)(ti),生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?NADH?經呼(hu)吸(xi)(xi)(xi)電子鏈(ETC)傳(chuan)遞(di)(di)把電子交給氧,在(zai)(zai)(zai)(zai)(zai)(zai)合(he)(he)成(cheng)(cheng)(cheng)(cheng)?ATP?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi)(shi),形(xing)成(cheng)(cheng)(cheng)(cheng)大(da)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?ROS,同(tong)時(shi)(shi)(shi)?NADH?再(zai)(zai)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)為(wei)(wei)(wei)(wei)(wei)?NAD+。糖、脂、蛋(dan)白(bai)(bai)質(zhi)(zhi)(zhi)三(san)大(da)代謝(xie)物(wu)質(zhi)(zhi)(zhi)分(fen)(fen)解(jie)(jie)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)氧化(hua)(hua)(hua)(hua)(hua)反(fan)(fan)應(ying)(ying)(ying)絕(jue)大(da)部分(fen)(fen)通(tong)(tong)過(guo)(guo)這一(yi)(yi)(yi)(yi)體(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi)完(wan)(wan)(wan)成(cheng)(cheng)(cheng)(cheng)。NAD(H)含(han)量(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)?NADH/NAD+比(bi)(bi)(bi)(bi)(bi)(bi)值(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)高(gao)低(di)可(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)評(ping)價糖酵(jiao)解(jie)(jie)和(he)(he)(he)(he)?TCA?循(xun)(xun)環(huan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)強弱。較高(gao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)NAD(H)及?NADH/NAD+比(bi)(bi)(bi)(bi)(bi)(bi)值(zhi)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)呼(hu)吸(xi)(xi)(xi)耗氧量(liang)(liang)(liang)(liang)(liang)(liang)較高(gao),處(chu)(chu)于(yu)(yu)(yu)(yu)過(guo)(guo)氧化(hua)(hua)(hua)(hua)(hua)狀(zhuang)(zhuang)態。此(ci)外,NADH/NAD+比(bi)(bi)(bi)(bi)(bi)(bi)值(zhi)升高(gao)也(ye)(ye)可(ke)(ke)(ke)抑制(zhi)糖酵(jiao)解(jie)(jie)和(he)(he)(he)(he)?TCA?循(xun)(xun)環(huan)。另外,NAD+降解(jie)(jie)產物(wu)對(dui)(dui)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)信(xin)號傳(chuan)導(dao)、代謝(xie)和(he)(he)(he)(he)基因表達(da)等具(ju)(ju)有(you)(you)重(zhong)(zhong)(zhong)(zhong)要(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)調控(kong)作(zuo)用(yong)(yong)(yong)(yong)(yong)。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)原(yuan)(yuan)(yuan)理(li)(li)(li)(li)(li)(li):分(fen)(fen)別(bie)(bie)用(yong)(yong)(yong)(yong)(yong)酸(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)和(he)(he)(he)(he)堿(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)?NADH,NADH?通(tong)(tong)過(guo)(guo)?PMS?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)遞(di)(di)氫作(zuo)用(yong)(yong)(yong)(yong)(yong),還(huan)原(yuan)(yuan)(yuan)氧化(hua)(hua)(hua)(hua)(hua)型(xing)(xing)(xing)噻唑藍(MTT)為(wei)(wei)(wei)(wei)(wei)甲(jia)瓚,在(zai)(zai)(zai)(zai)(zai)(zai)?570nm?下(xia)(xia)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)吸(xi)(xi)(xi)光(guang)(guang)值(zhi);而(er)(er)(er)(er)(er)(er)?NAD+可(ke)(ke)(ke)被(bei)乙醇脫氫酶(mei)(mei)(mei)還(huan)原(yuan)(yuan)(yuan)為(wei)(wei)(wei)(wei)(wei)?NADH,進(jin)(jin)一(yi)(yi)(yi)(yi)步(bu)采(cai)用(yong)(yong)(yong)(yong)(yong)?MTT?還(huan)原(yuan)(yuan)(yuan)法(fa)(fa)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。需(xu)(xu)(xu)(xu)自(zi)(zi)備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)儀(yi)器(qi)(qi)和(he)(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)品(pin)(pin)(pin):酶(mei)(mei)(mei)標(biao)(biao)儀(yi)、臺式離(li)(li)(li)心(xin)(xin)(xin)(xin)機、移(yi)(yi)液(ye)(ye)(ye)(ye)(ye)器(qi)(qi)、96?孔板(ban)、研(yan)缽、冰(bing)(bing)(bing)(bing)(bing)(bing)和(he)(he)(he)(he)蒸餾水(shui)(shui)。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)成(cheng)(cheng)(cheng)(cheng)和(he)(he)(he)(he)配制(zhi):酸(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping),4℃保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun);堿(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping),4℃保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi):液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)?10 mL×1?瓶(ping),4℃保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)二(er):液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)?3 mL×1?瓶(ping),4℃保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)三(san):粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping),-20?℃保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun),用(yong)(yong)(yong)(yong)(yong)時(shi)(shi)(shi)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?3mL?蒸餾水(shui)(shui),混(hun)勻(yun)(yun),用(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)完(wan)(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)一(yi)(yi)(yi)(yi)周(zhou);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)四:粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping),4?℃保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun),用(yong)(yong)(yong)(yong)(yong)時(shi)(shi)(shi)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?3mL?蒸餾水(shui)(shui),混(hun)勻(yun)(yun),用(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)完(wan)(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)一(yi)(yi)(yi)(yi)周(zhou);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)五:液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)?3.6mL×1?瓶(ping),4?℃保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu):液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)?30mL×1?瓶(ping),4?℃保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)七(qi):液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping),4?℃保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。NAD+和(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):1?血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(mL):酸(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(li)(li)(建議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)(yue)?0.1mL?血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL?酸(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)浴(yu)(yu)?5min(蓋(gai)緊,以(yi)(yi)(yi)(yi)(yi)防(fang)(fang)止(zhi)水(shui)(shui)分(fen)(fen)散失(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)卻(que)后(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?堿(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he),混(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(mL):堿(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(li)(li)(建議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)(yue)?0.1mL血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL?堿(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)浴(yu)(yu)?5min(蓋(gai)緊,以(yi)(yi)(yi)(yi)(yi)防(fang)(fang)止(zhi)水(shui)(shui)分(fen)(fen)散失(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)卻(que)后(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?酸(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he),混(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。2?組(zu)(zu)織(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)組(zu)(zu)織(zhi)(zhi)(zhi)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(g):酸(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(li)(li)(建議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)(yue)?0.1g?組(zu)(zu)織(zhi)(zhi)(zhi),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL酸(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)研(yan)磨,95℃水(shui)(shui)浴(yu)(yu)?5min(蓋(gai)緊,以(yi)(yi)(yi)(yi)(yi)防(fang)(fang)止(zhi)水(shui)(shui)分(fen)(fen)散失(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)卻(que)后(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?堿(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he),混(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)組(zu)(zu)織(zhi)(zhi)(zhi)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(g):堿(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(li)(li)(建議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)(yue)?0.1g?組(zu)(zu)織(zhi)(zhi)(zhi),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL堿(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)研(yan)磨,95℃水(shui)(shui)浴(yu)(yu)?5min(蓋(gai)緊,以(yi)(yi)(yi)(yi)(yi)防(fang)(fang)止(zhi)水(shui)(shui)分(fen)(fen)散失(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)卻(que)后(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?酸(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he),混(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3?細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先收集細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)到(dao)(dao)(dao)離(li)(li)(li)心(xin)(xin)(xin)(xin)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)內(nei),棄上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)數量(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)):酸(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(li)(li)(建議(yi)(yi)?500?萬細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL?酸(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),超(chao)聲(sheng)波破碎(冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu),功(gong)率20%或(huo)(huo)(huo)(huo)(huo)(huo)?200W,超(chao)聲(sheng)?3s,間隔(ge)?10s,重(zhong)(zhong)(zhong)(zhong)復?30?次(ci)(ci)(ci)),95℃水(shui)(shui)浴(yu)(yu)?5min(蓋(gai)緊,以(yi)(yi)(yi)(yi)(yi)防(fang)(fang)止(zhi)水(shui)(shui)分(fen)(fen)散失(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)卻(que)后(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?堿(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he),混(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先收集細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)到(dao)(dao)(dao)離(li)(li)(li)心(xin)(xin)(xin)(xin)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)內(nei),棄上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),按(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)數量(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)):堿(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)例(li)(li)(建議(yi)(yi)?500?萬細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL?堿(jian)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)),超(chao)聲(sheng)波破碎(冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu),功(gong)率20%或(huo)(huo)(huo)(huo)(huo)(huo)?200W,超(chao)聲(sheng)?3s,間隔(ge)?10s,重(zhong)(zhong)(zhong)(zhong)復?30?次(ci)(ci)(ci)),95℃水(shui)(shui)浴(yu)(yu)?5min(蓋(gai)緊,以(yi)(yi)(yi)(yi)(yi)防(fang)(fang)止(zhi)水(shui)(shui)分(fen)(fen)散失(shi)(shi)(shi));冰(bing)(bing)(bing)(bing)(bing)(bing)浴(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷(leng)(leng)卻(que)后(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?酸(suan)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)使(shi)(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he),混(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰(bing)(bing)(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。充分(fen)(fen)混(hun)勻(yun)(yun),靜置(zhi)(zhi)?5min?后(hou)(hou)(hou)(hou),20000g,25℃離(li)(li)(li)心(xin)(xin)(xin)(xin)?5min,棄上(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),沉淀中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru):混(hun)勻(yun)(yun),取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?200μL?轉移(yi)(yi)至微量(liang)(liang)(liang)(liang)(liang)(liang)石(shi)英比(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)皿(min)(min)(min)或(huo)(huo)(huo)(huo)(huo)(huo)?96?孔板(ban)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),570nm?下(xia)(xia)讀取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)對(dui)(dui)照(zhao)(zhao)(zhao)(zhao)吸(xi)(xi)(xi)光(guang)(guang)值(zhi)?A1?和(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)吸(xi)(xi)(xi)光(guang)(guang)值(zhi)?A2,計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)△A=A2-A1。注(zhu)(zhu)意(yi)(yi)事項:1、如(ru)(ru)(ru)(ru)果(guo)(guo)(guo)一(yi)(yi)(yi)(yi)次(ci)(ci)(ci)性(xing)(xing)(xing)(xing)(xing)(xing)(xing)(xing)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)數較多(duo),可(ke)(ke)(ke)將試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)、二(er)、三(san)和(he)(he)(he)(he)四按(an)(an)(an)(an)比(bi)(bi)(bi)(bi)(bi)(bi)例(li)(li)配成(cheng)(cheng)(cheng)(cheng)混(hun)合(he)(he)液(ye)(ye)(ye)(ye)(ye)。2、對(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)和(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)步(bu)驟(zou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie)(bie):對(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)加(jia)(jia)(jia)(jia)(jia)(jia)完(wan)(wan)(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)、二(er)、三(san)、四和(he)(he)(he)(he)五后(hou)(hou)(hou)(hou)必須馬(ma)上(shang)(shang)(shang)(shang)(shang)(shang)加(jia)(jia)(jia)(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu);測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)加(jia)(jia)(jia)(jia)(jia)(jia)完(wan)(wan)(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)、二(er)、三(san)、四和(he)(he)(he)(he)五后(hou)(hou)(hou)(hou)必須反(fan)(fan)應(ying)(ying)(ying)?20min?后(hou)(hou)(hou)(hou)再(zai)(zai)加(jia)(jia)(jia)(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu)。3、反(fan)(fan)應(ying)(ying)(ying)過(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)注(zhu)(zhu)意(yi)(yi)避(bi)光(guang)(guang)。4、若?NAD+測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0302,NADH?測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0222,說(shuo)(shuo)(shuo)明(ming)(ming)(ming)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)輔酶(mei)(mei)(mei)含(han)量(liang)(liang)(liang)(liang)(liang)(liang)較低(di),已低(di)于(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian),可(ke)(ke)(ke)做(zuo)如(ru)(ru)(ru)(ru)下(xia)(xia)調整(zheng):(1)將測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)避(bi)光(guang)(guang)靜置(zhi)(zhi)時(shi)(shi)(shi)間?20min?延長到(dao)(dao)(dao)?60min;(2)在(zai)(zai)(zai)(zai)(zai)(zai)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)階段增(zeng)加(jia)(jia)(jia)(jia)(jia)(jia)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)(liang)(liang)(liang)(liang),即取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?0.2g?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)或(huo)(huo)(huo)(huo)(huo)(huo)?0.2mL?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL?提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)。5、由(you)于(yu)(yu)(yu)(yu)每(mei)(mei)一(yi)(yi)(yi)(yi)個(ge)(ge)(ge)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)需(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)(yao)設(she)一(yi)(yi)(yi)(yi)個(ge)(ge)(ge)對(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan),本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)?100?管(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)(guan)保(bao)(bao)(bao)(bao)(bao)(bao)(bao)(bao)證測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)?48?個(ge)(ge)(ge)?NAD+或(huo)(huo)(huo)(huo)(huo)(huo)?NADH.NAD+和(he)(he)(he)(he)?NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan):(一(yi)(yi)(yi)(yi)) NAD+含(han)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)標(biao)(biao)準條件(jian)下(xia)(xia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回歸曲(qu)線為(wei)(wei)(wei)(wei)(wei)?y = 0.1475x + 0.0302,R2 = 0.9978;其(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)(wei)(wei)?NAD+濃(nong)(nong)度(du)(du)(du)?nmol/mL1、血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)量(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)=[(△A -0.0302)÷0.1475×V1) ]÷(V3×V1÷V2)=135.6×(△A -0.0302)2、組(zu)(zu)織(zhi)(zhi)(zhi)、細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)量(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(1)按(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋(dan)白(bai)(bai)濃(nong)(nong)度(du)(du)(du)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+(nmol/mg prot)=[(△A -0.0302)÷0.1475×V1)]÷(V1×Cpr)= 6.8×(△A -0.0302)÷Cpr(2)按(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)鮮(xian)重(zhong)(zhong)(zhong)(zhong)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+(nmol/g?鮮(xian)重(zhong)(zhong)(zhong)(zhong))= [(△A -0.0302)÷0.1475×V1)]÷(W×V1÷V2)= 13.6×(△A -0.0302)÷W(3)按(an)(an)(an)(an)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)密度(du)(du)(du)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+(nmol/104 cell)=[(△A -0.0302)÷0.1475×V1)]÷(500×V1÷V2)=0.027×(△A -0.0302)(二(er))NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)標(biao)(biao)準條件(jian)下(xia)(xia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回歸曲(qu)線為(wei)(wei)(wei)(wei)(wei)?y = 0.1404x + 0.0222,R2 = 0.9976;其(qi)(qi)(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)(wei)(wei)?NADH?濃(nong)(nong)度(du)(du)(du)?nmol/mL1、血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)= [ (△A -0.0222)÷0.1404×V1) ]÷(V3×V1÷V2)= 142.5×(△A -0.0222)2、組(zu)(zu)織(zhi)(zhi)(zhi)、細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(1)按(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋(dan)白(bai)(bai)濃(nong)(nong)度(du)(du)(du)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH (nmol/mg prot)= [ (△A -0.0222)÷0.1404×V1) ]÷(V1×Cpr)= 7.1×(△A -0.0222)÷Cpr(2)按(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)鮮(xian)重(zhong)(zhong)(zhong)(zhong)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH (nmol/g?鮮(xian)重(zhong)(zhong)(zhong)(zhong))=[(△A -0.0222)÷0.1404×V1) ]÷(W×V1÷V2)= 14.2×(△A -0.0222)÷W(3)按(an)(an)(an)(an)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)密度(du)(du)(du)計(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH (nmol/104 cell)= [ (△A -0.0222)÷0.1404×V1) ]÷(500×V1÷V2)=0.028×(△A -0.0222)V1:加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)反(fan)(fan)應(ying)(ying)(ying)體(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji),0.02mL;V2:加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji),2mL;V3:加(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)血(xue)(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(ji)(ji):0.1mL;Cpr:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋(dan)白(bai)(bai)質(zhi)(zhi)(zhi)濃(nong)(nong)度(du)(du)(du),mg/mL; W:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)質(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang),g;500:細(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)總數,500?萬。注(zhu)(zhu)?意(yi)(yi):?檢(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian)為(wei)(wei)(wei)(wei)(wei)?0.1nmol/mL?或(huo)(huo)(huo)(huo)(huo)(huo)?0.1nmol/g?鮮(xian)重(zhong)(zhong)(zhong)(zhong)?或(huo)(huo)(huo)(huo)(huo)(huo)?0.001nmol/mg prot
氧(yang)化(hua)(hua)(hua)型(xing)谷(gu)胱(guang)(guang)甘(gan)肽(tai)（GSSG）含(han)量(liang)(liang)(liang)(liang)(liang)(liang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)科(ke)研(yan)實(shi)(shi)驗(yan),不(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)臨(lin)(lin)床(chuang)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類型(xing)有(you)(you)(you)(you)哪些(xie)???生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)驗(yan)原(yuan)(yuan)理(li)(li)(li)(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)基于(yu)(yu)(yu)(yu)(yu)(yu)(yu)化(hua)(hua)(hua)學(xue)(xue)反(fan)應,理(li)(li)(li)(li)(li)論(lun)(lun)上(shang)(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)受生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)種(zhong)屬及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類型(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限制(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通(tong)常(chang)(chang)只(zhi)(zhi)(zhi)分(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類型(xing),不(bu)(bu)(bu)(bu)(bu)分(fen)(fen)種(zhong)屬。但(dan)(dan)是(shi)(shi)(shi)(shi)(shi)(shi)某(mou)些(xie)指(zhi)標(biao)(biao)(biao)在(zai)(zai)不(bu)(bu)(bu)(bu)(bu)同(tong)種(zhong)屬或(huo)(huo)(huo)(huo)類型(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方(fang)式不(bu)(bu)(bu)(bu)(bu)同(tong)。因(yin)此(ci)(ci),具體(ti)(ti)(ti)(ti)(ti)(ti)(ti)情(qing)況(kuang)請(qing)參(can)見(jian)(jian)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)或(huo)(huo)(huo)(huo)與技(ji)(ji)(ji)術支持(chi)聯(lian)系(xi)(xi)(xi)確(que)認(ren)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)般要(yao)(yao)(yao)(yao)(yao)求(qiu):1)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)言(yan),樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)無(wu)溶(rong)血(xue)(xue)(xue)、脂(zhi)血(xue)(xue)(xue)、的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現象。組(zu)(zu)織樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)將(jiang)(jiang)血(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)洗凈擦(ca)干(gan)后(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)稱重(zhong)(zhong)處(chu)(chu)理(li)(li)(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)處(chu)(chu)理(li)(li)(li)(li)(li)后(hou)(hou)(hou)(hou)盡(jin)快進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且(qie)(qie)保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)盒(he)(he)(he)之(zhi)上(shang)(shang)(shang)(shang)(shang),待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。求(qiu)3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)澄清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),若(ruo)不(bu)(bu)(bu)(bu)(bu)澄清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),需(xu)(xu)離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)后(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特(te)殊指(zhi)標(biao)(biao)(biao)除(chu)外(wai)(wai))。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)值(zhi)(zhi)(zhi)(zhi)應在(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)線(xian)性(xing)(xing)范(fan)(fan)圍內(nei),若(ruo)超(chao)(chao)過(guo)(guo)線(xian)性(xing)(xing)范(fan)(fan)圍,需(xu)(xu)稀(xi)釋后(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若(ruo)低(di)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)(fan)圍,需(xu)(xu)增加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)濃(nong)(nong)度(du)(du)(du)(du)后(hou)(hou)(hou)(hou)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。微(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)(fa)和(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)光(guang)(guang)光(guang)(guang)度(du)(du)(du)(du)法(fa)(fa)(fa)(fa)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區(qu)別?分(fen)(fen)光(guang)(guang)光(guang)(guang)度(du)(du)(du)(du)法(fa)(fa)(fa)(fa):主(zhu)流(liu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)1mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)(yi)器分(fen)(fen)光(guang)(guang)光(guang)(guang)度(du)(du)(du)(du)計(ji)(ji)(ji)(ji),能(neng)夠大(da)(da)(da)限度(du)(du)(du)(du)地滿足絕(jue)大(da)(da)(da)多數(shu)用(yong)(yong)(yong)(yong)(yong)(yong)戶的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)需(xu)(xu)要(yao)(yao)(yao)(yao)(yao)。微(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)(fa):主(zhu)流(liu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)0.2mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)(yi)器或(huo)(huo)(huo)(huo)分(fen)(fen)光(guang)(guang)光(guang)(guang)度(du)(du)(du)(du)計(ji)(ji)(ji)(ji),測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)更加(jia)(jia)(jia)簡便快捷。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)規格(ge)(ge)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)是(shi)(shi)(shi)(shi)(shi)(shi)100管(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)50管(guan)(guan)(guan)是(shi)(shi)(shi)(shi)(shi)(shi)什么意(yi)思?(1)50管(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):50管(guan)(guan)(guan)指(zhi)能(neng)夠做(zuo)(zuo)(zuo)50次(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)48個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)。如(ru)(ru)果(guo)(guo)每(mei)個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)重(zhong)(zhong)復(fu)(fu)(fu)(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci),那么只(zhi)(zhi)(zhi)能(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)16個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)。其中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)(you)2次(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)空(kong)白管(guan)(guan)(guan)或(huo)(huo)(huo)(huo)者(zhe)對照(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)。值(zhi)(zhi)(zhi)(zhi)得(de)特(te)別注(zhu)意(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi),50管(guan)(guan)(guan)/24樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由(you)(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)每(mei)個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)(dou)(dou)需(xu)(xu)要(yao)(yao)(yao)(yao)(yao)做(zuo)(zuo)(zuo)對照(zhao)(zhao)(zhao)(zhao)(zhao),因(yin)此(ci)(ci)能(neng)夠測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)僅(jin)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)8個(ge)(ge)(ge)(ge)(假(jia)設(she)做(zuo)(zuo)(zuo)3次(ci)重(zhong)(zhong)復(fu)(fu)(fu)(fu))(2)100管(guan)(guan)(guan)/96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):100管(guan)(guan)(guan)指(zhi)能(neng)夠做(zuo)(zuo)(zuo)100次(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)96個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin),如(ru)(ru)果(guo)(guo)每(mei)個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)重(zhong)(zhong)復(fu)(fu)(fu)(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci),那么只(zhi)(zhi)(zhi)能(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)32個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)。其中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)(you)4次(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)空(kong)白管(guan)(guan)(guan)或(huo)(huo)(huo)(huo)者(zhe)對照(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)。注(zhu)意(yi)由(you)(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)瓶(ping)(ping)(ping)璧和(he)(he)(he)(he)(he)(he)(he)移液(ye)(ye)(ye)(ye)(ye)(ye)(ye)器吸(xi)附等(deng),通(tong)常(chang)(chang)要(yao)(yao)(yao)(yao)(yao)比(bi)理(li)(li)(li)(li)(li)論(lun)(lun)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)次(ci)數(shu)少1~3次(ci)。其它(ta)規格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)義(yi)依次(ci)類推(tui)。值(zhi)(zhi)(zhi)(zhi)得(de)特(te)別注(zhu)意(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi),100管(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由(you)(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)每(mei)個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)(dou)(dou)需(xu)(xu)要(yao)(yao)(yao)(yao)(yao)做(zuo)(zuo)(zuo)對照(zhao)(zhao)(zhao)(zhao)(zhao),因(yin)此(ci)(ci)能(neng)夠測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)僅(jin)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)16個(ge)(ge)(ge)(ge)(假(jia)設(she)做(zuo)(zuo)(zuo)3次(ci)重(zhong)(zhong)復(fu)(fu)(fu)(fu))。如(ru)(ru)何防(fang)止試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)失效(xiao)?????不(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)(yao)過(guo)(guo)早訂購(gou),以(yi)(yi)(yi)免試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)失效(xiao).收到(dao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)后(hou)(hou)(hou)(hou),檢(jian)(jian)(jian)(jian)查外(wai)(wai)包(bao)裝(zhuang)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)正(zheng)常(chang)(chang)。馬(ma)(ma)(ma)上(shang)(shang)(shang)(shang)(shang)打(da)開,按(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)核對試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)正(zheng)確(que),注(zhu)意(yi)瓶(ping)(ping)(ping)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)狀(zhuang)態是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)與說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)一(yi)(yi)(yi)(yi)(yi)致(zhi)。如(ru)(ru)果(guo)(guo)有(you)(you)(you)(you)問題(ti),或(huo)(huo)(huo)(huo)者(zhe)懷疑,馬(ma)(ma)(ma)上(shang)(shang)(shang)(shang)(shang)聯(lian)系(xi)(xi)(xi)公(gong)(gong)司銷(xiao)售(shou),進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)確(que)認(ren)。如(ru)(ru)果(guo)(guo)沒有(you)(you)(you)(you)問題(ti),請(qing)務(wu)(wu)(wu)必(bi)按(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。注(zhu)意(yi),不(bu)(bu)(bu)(bu)(bu)同(tong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji),可(ke)(ke)(ke)(ke)(ke)(ke)能(neng)需(xu)(xu)要(yao)(yao)(yao)(yao)(yao)分(fen)(fen)別保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)在(zai)(zai)常(chang)(chang)溫(25°℃)、4℃和(he)(he)(he)(he)(he)(he)(he)-20℃,務(wu)(wu)(wu)必(bi)分(fen)(fen)別按(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)要(yao)(yao)(yao)(yao)(yao)求(qiu)保(bao)(bao)(bao)存(cun)(cun)(cun)(cun),不(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)(yao)整(zheng)個(ge)(ge)(ge)(ge)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)放置于(yu)(yu)(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)箱。如(ru)(ru)何進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)?(1)務(wu)(wu)(wu)必(bi)在(zai)(zai)7個(ge)(ge)(ge)(ge)工作(zuo)日內(nei)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),以(yi)(yi)(yi)確(que)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)該試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)適合您(nin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin),以(yi)(yi)(yi)免造成(cheng)(cheng)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)和(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浪(lang)費。(2)選擇2~3個(ge)(ge)(ge)(ge)預(yu)(yu)期(qi)差異比(bi)較大(da)(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin),嚴格(ge)(ge)按(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)操作(zuo),注(zhu)意(yi)儀(yi)(yi)器工作(zuo)狀(zhuang)態,操作(zuo)規范(fan)(fan),控(kong)制(zhi)(zhi)(zhi)好測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)條(tiao)件(jian),尤(you)其是(shi)(shi)(shi)(shi)(shi)(shi)溫度(du)(du)(du)(du)。(3)如(ru)(ru)實(shi)(shi)與公(gong)(gong)司銷(xiao)售(shou)溝通(tong)預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)果(guo)(guo),公(gong)(gong)司技(ji)(ji)(ji)術人員會(hui)確(que)認(ren)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)果(guo)(guo)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)正(zheng)常(chang)(chang),是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)需(xu)(xu)要(yao)(yao)(yao)(yao)(yao)調(diao)(diao)整(zheng),如(ru)(ru)何進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)調(diao)(diao)整(zheng),從而(er)確(que)保(bao)(bao)(bao)您(nin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)果(guo)(guo)可(ke)(ke)(ke)(ke)(ke)(ke)靠。比(bi)色(se)(se)(se)(se)皿(min)(min)(min)(min)有(you)(you)(you)(you)哪些(xie)規格(ge)(ge)?(1)常(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)比(bi)色(se)(se)(se)(se)皿(min)(min)(min)(min),如(ru)(ru)1mL和(he)(he)(he)(he)(he)(he)(he)3mL等(deng),常(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)都(dou)(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)選用(yong)(yong)(yong)(yong)(yong)(yong)1mL體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi),只(zhi)(zhi)(zhi)能(neng)用(yong)(yong)(yong)(yong)(yong)(yong)1mL比(bi)色(se)(se)(se)(se)皿(min)(min)(min)(min),不(bu)(bu)(bu)(bu)(bu)可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong)更大(da)(da)(da)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)色(se)(se)(se)(se)皿(min)(min)(min)(min),否(fou)則(ze)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)量(liang)(liang)(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)(bu)夠,無(wu)法(fa)(fa)(fa)(fa)正(zheng)常(chang)(chang)比(bi)色(se)(se)(se)(se)。(2)微(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)比(bi)色(se)(se)(se)(se)皿(min)(min)(min)(min),如(ru)(ru)0.25mL和(he)(he)(he)(he)(he)(he)(he)0.5mL等(deng),微(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)都(dou)(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)選用(yong)(yong)(yong)(yong)(yong)(yong)0.25mL比(bi)色(se)(se)(se)(se)皿(min)(min)(min)(min)。一(yi)(yi)(yi)(yi)(yi)般客戶都(dou)(dou)(dou)選擇使(shi)用(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)儀(yi)(yi)(需(xu)(xu)要(yao)(yao)(yao)(yao)(yao)自(zi)(zi)備酶(mei)(mei)(mei)標(biao)(biao)(biao)板)(3)紫外(wai)(wai)波長檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)需(xu)(xu)要(yao)(yao)(yao)(yao)(yao)石(shi)英比(bi)色(se)(se)(se)(se)皿(min)(min)(min)(min),不(bu)(bu)(bu)(bu)(bu)可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong)普通(tong)玻璃比(bi)色(se)(se)(se)(se)皿(min)(min)(min)(min)。(4)不(bu)(bu)(bu)(bu)(bu)同(tong)規格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)色(se)(se)(se)(se)皿(min)(min)(min)(min),其外(wai)(wai)觀(guan)大(da)(da)(da)小(xiao)是(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)(yi)(yi)(yi)致(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de),都(dou)(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)1cm光(guang)(guang)徑(jing)(也(ye)(ye)有(you)(you)(you)(you)0.5cm光(guang)(guang)徑(jing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)色(se)(se)(se)(se)皿(min)(min)(min)(min),不(bu)(bu)(bu)(bu)(bu)常(chang)(chang)見(jian)(jian),也(ye)(ye)可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong),但(dan)(dan)是(shi)(shi)(shi)(shi)(shi)(shi)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)時(shi)(shi)(shi)要(yao)(yao)(yao)(yao)(yao)注(zhu)意(yi)修改計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)公(gong)(gong)式),其內(nei)容積差異在(zai)(zai)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)壁的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)厚(hou)度(du)(du)(du)(du)。代測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)優(you)點(dian)(1)更加(jia)(jia)(jia)省事兒,完(wan)全(quan)不(bu)(bu)(bu)(bu)(bu)必(bi)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)犯(fan)難。用(yong)(yong)(yong)(yong)(yong)(yong)戶只(zhi)(zhi)(zhi)要(yao)(yao)(yao)(yao)(yao)提(ti)(ti)(ti)(ti)(ti)(ti)供樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)和(he)(he)(he)(he)(he)(he)(he)想測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)指(zhi)標(biao)(biao)(biao),就不(bu)(bu)(bu)(bu)(bu)再(zai)需(xu)(xu)要(yao)(yao)(yao)(yao)(yao)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)操心(xin)(xin)(xin)(xin)(xin)(xin)了,不(bu)(bu)(bu)(bu)(bu)再(zai)受到(dao)操作(zuo)技(ji)(ji)(ji)能(neng)、儀(yi)(yi)器設(she)備和(he)(he)(he)(he)(he)(he)(he)時(shi)(shi)(shi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限制(zhi)(zhi)(zhi),就等(deng)著(zhu)拿(na)到(dao)數(shu)據(ju)寫論(lun)(lun)文。(2)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)數(shu)據(ju)更有(you)(you)(you)(you)保(bao)(bao)(bao)障(zhang)。嚴格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)管(guan)(guan)(guan)理(li)(li)(li)(li)(li)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi),從而(er)保(bao)(bao)(bao)證了測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結(jie)果(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)真實(shi)(shi)可(ke)(ke)(ke)(ke)(ke)(ke)靠。我(wo)們還(huan)建(jian)立了免費預(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)制(zhi)(zhi)(zhi)度(du)(du)(du)(du)。(3)防(fang)止樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)損失無(wu)論(lun)(lun)是(shi)(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)戶自(zi)(zi)己(ji)完(wan)成(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)全(quan)過(guo)(guo)程,還(huan)是(shi)(shi)(shi)(shi)(shi)(shi)購(gou)買試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)完(wan)成(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),都(dou)(dou)(dou)可(ke)(ke)(ke)(ke)(ke)(ke)能(neng)因(yin)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)缺乏測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)經(jing)驗(yan),導致(zhi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)失敗,從而(er)損失樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情(qing)況(kuang)。對于(yu)(yu)(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)命(ming)科(ke)學(xue)(xue)研(yan)究(jiu)而(er)言(yan),樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)是(shi)(shi)(shi)(shi)(shi)(shi)＊珍貴的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)一(yi)(yi)(yi)(yi)(yi)旦損失,輕則(ze)造成(cheng)(cheng)研(yan)究(jiu)進(jin)(jin)(jin)(jin)度(du)(du)(du)(du)被(bei)(bei)耽(dan)擱,重(zhong)(zhong)則(ze)造成(cheng)(cheng)研(yan)究(jiu)完(wan)全(quan)失敗!代測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)能(neng)夠充(chong)分(fen)(fen)保(bao)(bao)(bao)證測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)功(gong)(gong),從而(er)防(fang)止了樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)損失。當然,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)功(gong)(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前提(ti)(ti)(ti)(ti)(ti)(ti),而(er)且(qie)(qie)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)只(zhi)(zhi)(zhi)有(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)戶自(zi)(zi)己(ji)才能(neng)控(kong)制(zhi)(zhi)(zhi)和(he)(he)(he)(he)(he)(he)(he)保(bao)(bao)(bao)證。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)原(yuan)(yuan)理(li)(li)(li)(li)(li)??生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he),又稱生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he),是(shi)(shi)(shi)(shi)(shi)(shi)利用(yong)(yong)(yong)(yong)(yong)(yong)特(te)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)成(cheng)(cheng)分(fen)(fen)、動(dong)(dong)力學(xue)(xue)及(ji)化(hua)(hua)(hua)學(xue)(xue)反(fan)應等(deng)技(ji)(ji)(ji)術制(zhi)(zhi)(zhi)備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he),用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)理(li)(li)(li)(li)(li)學(xue)(xue)、病理(li)(li)(li)(li)(li)學(xue)(xue)、藥理(li)(li)(li)(li)(li)學(xue)(xue)等(deng)領域(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)特(te)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)分(fen)(fen)子或(huo)(huo)(huo)(huo)化(hua)(hua)(hua)學(xue)(xue)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)包(bao)括(kuo)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、酶(mei)(mei)(mei)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)、探針(zhen)等(deng)多個(ge)(ge)(ge)(ge)組(zu)(zu)成(cheng)(cheng)部分(fen)(fen),其中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)＊常(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)法(fa)(fa)(fa)(fa)。底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)法(fa)(fa)(fa)(fa)是(shi)(shi)(shi)(shi)(shi)(shi)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)原(yuan)(yuan)理(li)(li)(li)(li)(li)。在(zai)(zai)該過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)與酶(mei)(mei)(mei)在(zai)(zai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)膜上(shang)(shang)(shang)(shang)(shang)結(jie)合形(xing)成(cheng)(cheng)基質(zhi)(zhi)酶(mei)(mei)(mei),進(jin)(jin)(jin)(jin)而(er)形(xing)成(cheng)(cheng)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)復(fu)(fu)(fu)(fu)合物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)。隨著(zhu)時(shi)(shi)(shi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)推(tui)移,底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)(mei)復(fu)(fu)(fu)(fu)合物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)逐漸(jian)分(fen)(fen)解,釋放出分(fen)(fen)子小(xiao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)子大(da)(da)(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)未完(wan)全(quan)消化(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)。未完(wan)全(quan)消化(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)被(bei)(bei)一(yi)(yi)(yi)(yi)(yi)系(xi)(xi)(xi)列的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)(mei)代謝,同(tong)時(shi)(shi)(shi)底(di)(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、酶(mei)(mei)(mei)和(he)(he)(he)(he)(he)(he)(he)代謝產物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)都(dou)(dou)(dou)會(hui)對復(fu)(fu)(fu)(fu)合物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)穩定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)產生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)影響(xiang),從而(er)影響(xiang)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)(guo)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)可(ke)(ke)(ke)(ke)(ke)(ke)以(yi)(yi)(yi)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類型(xing)有(you)(you)(you)(you)哪些(xie)?? ?生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)驗(yan)原(yuan)(yuan)理(li)(li)(li)(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)基于(yu)(yu)(yu)(yu)(yu)(yu)(yu)化(hua)(hua)(hua)學(xue)(xue)反(fan)應,理(li)(li)(li)(li)(li)論(lun)(lun)上(shang)(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)受生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)種(zhong)屬及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類型(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限制(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通(tong)常(chang)(chang)只(zhi)(zhi)(zhi)分(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)類型(xing),不(bu)(bu)(bu)(bu)(bu)分(fen)(fen)種(zhong)屬。但(dan)(dan)是(shi)(shi)(shi)(shi)(shi)(shi)某(mou)些(xie)指(zhi)標(biao)(biao)(biao)在(zai)(zai)不(bu)(bu)(bu)(bu)(bu)同(tong)種(zhong)屬或(huo)(huo)(huo)(huo)類型(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方(fang)式不(bu)(bu)(bu)(bu)(bu)同(tong)。因(yin)此(ci)(ci),具體(ti)(ti)(ti)(ti)(ti)(ti)(ti)情(qing)況(kuang)請(qing)參(can)見(jian)(jian)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)書(shu)或(huo)(huo)(huo)(huo)與技(ji)(ji)(ji)術支持(chi)聯(lian)系(xi)(xi)(xi)確(que)認(ren)。生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)(yi)般要(yao)(yao)(yao)(yao)(yao)求(qiu):1)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)化(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)言(yan),樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)＊-好無(wu)溶(rong)血(xue)(xue)(xue)、脂(zhi)血(xue)(xue)(xue)、黃-疸(dan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現象。組(zu)(zu)織樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)將(jiang)(jiang)血(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)洗凈擦(ca)干(gan)后(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)稱重(zhong)(zhong)處(chu)(chu)理(li)(li)(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)處(chu)(chu)理(li)(li)(li)(li)(li)后(hou)(hou)(hou)(hou)＊-好盡(jin)快進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且(qie)(qie)保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)冰(bing)(bing)(bing)(bing)盒(he)(he)(he)之(zhi)上(shang)(shang)(shang)(shang)(shang),待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)澄清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),若(ruo)不(bu)(bu)(bu)(bu)(bu)澄清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),需(xu)(xu)離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)后(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特(te)殊指(zhi)標(biao)(biao)(biao)除(chu)外(wai)(wai))。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)值(zhi)(zhi)(zhi)(zhi)應在(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)線(xian)性(xing)(xing)范(fan)(fan)圍內(nei),若(ruo)超(chao)(chao)過(guo)(guo)線(xian)性(xing)(xing)范(fan)(fan)圍,需(xu)(xu)稀(xi)釋后(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若(ruo)低(di)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)(fan)圍,需(xu)(xu)增加(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)濃(nong)(nong)度(du)(du)(du)(du)后(hou)(hou)(hou)(hou)進(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。氧(yang)化(hua)(hua)(hua)型(xing)谷(gu)胱(guang)(guang)甘(gan)肽(tai)（GSSG）含(han)量(liang)(liang)(liang)(liang)(liang)(liang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)科(ke)研(yan)實(shi)(shi)驗(yan),不(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)臨(lin)(lin)床(chuang)。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)意(yi)義(yi):輔(fu)酶(mei)(mei)(mei)NAD(H)廣泛存(cun)(cun)(cun)(cun)在(zai)(zai)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)動(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、植物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、微(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)培(pei)養細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),NAD+是(shi)(shi)(shi)(shi)(shi)(shi)糖(tang)酵解(EMP)和(he)(he)(he)(he)(he)(he)(he)三羧酸(suan)(suan)循(xun)環(huan)(TCA)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主(zhu)要(yao)(yao)(yao)(yao)(yao)氫受體(ti)(ti)(ti)(ti)(ti)(ti)(ti),生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)成(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?NADH?經(jing)呼(hu)(hu)吸(xi)電子鏈(ETC)傳遞把電子交給氧(yang),在(zai)(zai)合成(cheng)(cheng)?ATP?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)時(shi)(shi)(shi),形(xing)成(cheng)(cheng)大(da)(da)(da)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?ROS,同(tong)時(shi)(shi)(shi)?NADH?再(zai)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?NAD+。糖(tang)、脂(zhi)、蛋白質(zhi)(zhi)三大(da)(da)(da)代謝物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)分(fen)(fen)解中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)氧(yang)化(hua)(hua)(hua)反(fan)應絕(jue)大(da)(da)(da)部分(fen)(fen)通(tong)過(guo)(guo)這一(yi)(yi)(yi)(yi)(yi)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi)完(wan)成(cheng)(cheng)。NAD(H)含(han)量(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)(he)(he)?NADH/NAD+比(bi)值(zhi)(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)高低(di)可(ke)(ke)(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)評價糖(tang)酵解和(he)(he)(he)(he)(he)(he)(he)?TCA?循(xun)環(huan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)強弱。較高的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)NAD(H)及(ji)?NADH/NAD+比(bi)值(zhi)(zhi)(zhi)(zhi)說(shuo)(shuo)(shuo)明(ming)(ming)(ming)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)呼(hu)(hu)吸(xi)耗氧(yang)量(liang)(liang)(liang)(liang)(liang)(liang)較高,處(chu)(chu)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)過(guo)(guo)氧(yang)化(hua)(hua)(hua)狀(zhuang)態。此(ci)(ci)外(wai)(wai),NADH/NAD+比(bi)值(zhi)(zhi)(zhi)(zhi)升(sheng)高也(ye)(ye)可(ke)(ke)(ke)(ke)(ke)(ke)抑制(zhi)(zhi)(zhi)糖(tang)酵解和(he)(he)(he)(he)(he)(he)(he)?TCA?循(xun)環(huan)。另外(wai)(wai),NAD+降(jiang)解產物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)對細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)信號傳導、代謝和(he)(he)(he)(he)(he)(he)(he)基因(yin)表達等(deng)具有(you)(you)(you)(you)重(zhong)(zhong)要(yao)(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)調(diao)(diao)控(kong)作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)原(yuan)(yuan)理(li)(li)(li)(li)(li):分(fen)(fen)別用(yong)(yong)(yong)(yong)(yong)(yong)酸(suan)(suan)性(xing)(xing)和(he)(he)(he)(he)(he)(he)(he)堿(jian)(jian)(jian)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH,NADH?通(tong)過(guo)(guo)?PMS?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)遞氫作(zuo)用(yong)(yong)(yong)(yong)(yong)(yong),還(huan)原(yuan)(yuan)氧(yang)化(hua)(hua)(hua)型(xing)噻(sai)唑藍(MTT)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)甲瓚,在(zai)(zai)?570nm?下(xia)(xia)(xia)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)吸(xi)光(guang)(guang)值(zhi)(zhi)(zhi)(zhi);而(er)?NAD+可(ke)(ke)(ke)(ke)(ke)(ke)被(bei)(bei)乙醇脫氫酶(mei)(mei)(mei)還(huan)原(yuan)(yuan)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?NADH,進(jin)(jin)(jin)(jin)一(yi)(yi)(yi)(yi)(yi)步(bu)采用(yong)(yong)(yong)(yong)(yong)(yong)?MTT?還(huan)原(yuan)(yuan)法(fa)(fa)(fa)(fa)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。需(xu)(xu)自(zi)(zi)備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)儀(yi)(yi)器和(he)(he)(he)(he)(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)品(pin)(pin)(pin)(pin):酶(mei)(mei)(mei)標(biao)(biao)(biao)儀(yi)(yi)、臺式離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)機、移液(ye)(ye)(ye)(ye)(ye)(ye)(ye)器、96?孔板、研(yan)缽、冰(bing)(bing)(bing)(bing)和(he)(he)(he)(he)(he)(he)(he)蒸(zheng)餾水(shui)。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)成(cheng)(cheng)和(he)(he)(he)(he)(he)(he)(he)配制(zhi)(zhi)(zhi):酸(suan)(suan)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping)(ping),4℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun);堿(jian)(jian)(jian)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping)(ping),4℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?10 mL×1?瓶(ping)(ping)(ping),4℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)二(er):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?3 mL×1?瓶(ping)(ping)(ping),4℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)三:粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping)(ping)(ping),-20?℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun),用(yong)(yong)(yong)(yong)(yong)(yong)時(shi)(shi)(shi)加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?3mL?蒸(zheng)餾水(shui),混(hun)(hun)勻(yun)(yun),用(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)一(yi)(yi)(yi)(yi)(yi)周;試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)四(si):粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping)(ping)(ping),4?℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun),用(yong)(yong)(yong)(yong)(yong)(yong)時(shi)(shi)(shi)加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?3mL?蒸(zheng)餾水(shui),混(hun)(hun)勻(yun)(yun),用(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)一(yi)(yi)(yi)(yi)(yi)周;試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)五(wu):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?3.6mL×1?瓶(ping)(ping)(ping),4?℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?30mL×1?瓶(ping)(ping)(ping),4?℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)七:液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping)(ping),4?℃保(bao)(bao)(bao)存(cun)(cun)(cun)(cun)。NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):1?血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(mL):酸(suan)(suan)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(mL)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(建(jian)議取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)(yue)(yue)?0.1mL?血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL?酸(suan)(suan)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)),95℃水(shui)浴(yu)?5min(蓋(gai)緊(jin)(jin),以(yi)(yi)(yi)防(fang)止水(shui)分(fen)(fen)散(san)失);冰(bing)(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻后(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)(jian)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(mL):堿(jian)(jian)(jian)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(mL)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(建(jian)議取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)(yue)(yue)?0.1mL血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL?堿(jian)(jian)(jian)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)),95℃水(shui)浴(yu)?5min(蓋(gai)緊(jin)(jin),以(yi)(yi)(yi)防(fang)止水(shui)分(fen)(fen)散(san)失);冰(bing)(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻后(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?酸(suan)(suan)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。2?組(zu)(zu)織中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)組(zu)(zu)織質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(g):酸(suan)(suan)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(mL)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(建(jian)議取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)(yue)(yue)?0.1g?組(zu)(zu)織,加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL酸(suan)(suan)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)浴(yu)研(yan)磨,95℃水(shui)浴(yu)?5min(蓋(gai)緊(jin)(jin),以(yi)(yi)(yi)防(fang)止水(shui)分(fen)(fen)散(san)失);冰(bing)(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻后(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)(jian)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)組(zu)(zu)織質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(g):堿(jian)(jian)(jian)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(mL)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(建(jian)議取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)(yue)(yue)?0.1g?組(zu)(zu)織,加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL堿(jian)(jian)(jian)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)浴(yu)研(yan)磨,95℃水(shui)浴(yu)?5min(蓋(gai)緊(jin)(jin),以(yi)(yi)(yi)防(fang)止水(shui)分(fen)(fen)散(san)失);冰(bing)(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻后(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?酸(suan)(suan)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3?細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先收集(ji)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)到(dao)離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)管(guan)(guan)(guan)內(nei),棄上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),按(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)(ge)):酸(suan)(suan)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(mL)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(建(jian)議?500?萬細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL?酸(suan)(suan)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)),超(chao)(chao)聲(sheng)(sheng)(sheng)波破碎(sui)(冰(bing)(bing)(bing)(bing)浴(yu),功(gong)(gong)率20%或(huo)(huo)(huo)(huo)?200W,超(chao)(chao)聲(sheng)(sheng)(sheng)?3s,間(jian)隔?10s,重(zhong)(zhong)復(fu)(fu)(fu)(fu)?30?次(ci)),95℃水(shui)浴(yu)?5min(蓋(gai)緊(jin)(jin),以(yi)(yi)(yi)防(fang)止水(shui)分(fen)(fen)散(san)失);冰(bing)(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻后(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)(jian)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先收集(ji)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)到(dao)離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)管(guan)(guan)(guan)內(nei),棄上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),按(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)(ge)):堿(jian)(jian)(jian)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(mL)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)例(li)(建(jian)議?500?萬細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL?堿(jian)(jian)(jian)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)),超(chao)(chao)聲(sheng)(sheng)(sheng)波破碎(sui)(冰(bing)(bing)(bing)(bing)浴(yu),功(gong)(gong)率20%或(huo)(huo)(huo)(huo)?200W,超(chao)(chao)聲(sheng)(sheng)(sheng)?3s,間(jian)隔?10s,重(zhong)(zhong)復(fu)(fu)(fu)(fu)?30?次(ci)),95℃水(shui)浴(yu)?5min(蓋(gai)緊(jin)(jin),以(yi)(yi)(yi)防(fang)止水(shui)分(fen)(fen)散(san)失);冰(bing)(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻后(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?500μL?酸(suan)(suan)性(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)之(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun),10000g 4?℃離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),置冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)(shang)(shang)待(dai)(dai)(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。充(chong)分(fen)(fen)混(hun)(hun)勻(yun)(yun),靜置?5min?后(hou)(hou)(hou)(hou),20000g,25℃離(li)(li)(li)心(xin)(xin)(xin)(xin)(xin)(xin)?5min,棄上(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing),沉淀中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)入(ru)(ru)(ru)(ru):混(hun)(hun)勻(yun)(yun),取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?200μL?轉移至微(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)石(shi)英比(bi)色(se)(se)(se)(se)皿(min)(min)(min)(min)或(huo)(huo)(huo)(huo)?96?孔板中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),570nm?下(xia)(xia)(xia)讀取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)對照(zhao)(zhao)(zhao)(zhao)(zhao)吸(xi)光(guang)(guang)值(zhi)(zhi)(zhi)(zhi)?A1?和(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)吸(xi)光(guang)(guang)值(zhi)(zhi)(zhi)(zhi)?A2,計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)△A=A2-A1。注(zhu)意(yi)事項:1、如(ru)(ru)果(guo)(guo)一(yi)(yi)(yi)(yi)(yi)次(ci)性(xing)(xing)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)數(shu)較多,可(ke)(ke)(ke)(ke)(ke)(ke)將(jiang)(jiang)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)、二(er)、三和(he)(he)(he)(he)(he)(he)(he)四(si)按(an)(an)(an)(an)(an)比(bi)例(li)配成(cheng)(cheng)混(hun)(hun)合液(ye)(ye)(ye)(ye)(ye)(ye)(ye)。2、對照(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)步(bu)驟(zou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區(qu)別:對照(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)加(jia)(jia)(jia)完(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)、二(er)、三、四(si)和(he)(he)(he)(he)(he)(he)(he)五(wu)后(hou)(hou)(hou)(hou)必(bi)須馬(ma)(ma)(ma)上(shang)(shang)(shang)(shang)(shang)加(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu);測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)加(jia)(jia)(jia)完(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)(yi)、二(er)、三、四(si)和(he)(he)(he)(he)(he)(he)(he)五(wu)后(hou)(hou)(hou)(hou)必(bi)須反(fan)應?20min?后(hou)(hou)(hou)(hou)再(zai)加(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu)。3、反(fan)應過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)注(zhu)意(yi)避光(guang)(guang)。4、若(ruo)?NAD+測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0302,NADH?測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0222,說(shuo)(shuo)(shuo)明(ming)(ming)(ming)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)輔(fu)酶(mei)(mei)(mei)含(han)量(liang)(liang)(liang)(liang)(liang)(liang)較低(di),已低(di)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限,可(ke)(ke)(ke)(ke)(ke)(ke)做(zuo)(zuo)(zuo)如(ru)(ru)下(xia)(xia)(xia)調(diao)(diao)整(zheng):(1)將(jiang)(jiang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)避光(guang)(guang)靜置時(shi)(shi)(shi)間(jian)?20min?延(yan)長到(dao)?60min;(2)在(zai)(zai)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)階(jie)段增加(jia)(jia)(jia)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)(liang)(liang)(liang)(liang),即取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?0.2g?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)或(huo)(huo)(huo)(huo)?0.2mL?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)?1mL?提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)。5、由(you)(you)于(yu)(yu)(yu)(yu)(yu)(yu)(yu)每(mei)一(yi)(yi)(yi)(yi)(yi)個(ge)(ge)(ge)(ge)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)需(xu)(xu)要(yao)(yao)(yao)(yao)(yao)設(she)一(yi)(yi)(yi)(yi)(yi)個(ge)(ge)(ge)(ge)對照(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan),本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)?100?管(guan)(guan)(guan)保(bao)(bao)(bao)證測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)?48?個(ge)(ge)(ge)(ge)?NAD+或(huo)(huo)(huo)(huo)?NADH.NAD+和(he)(he)(he)(he)(he)(he)(he)?NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan):(一(yi)(yi)(yi)(yi)(yi)) NAD+含(han)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)標(biao)(biao)(biao)準條(tiao)件(jian)下(xia)(xia)(xia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回歸曲線(xian)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?y = 0.1475x + 0.0302,R2 = 0.9978;其中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?NAD+濃(nong)(nong)度(du)(du)(du)(du)?nmol/mL1、血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)量(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)=[(△A -0.0302)÷0.1475×V1) ]÷(V3×V1÷V2)=135.6×(△A -0.0302)2、組(zu)(zu)織、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)量(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(1)按(an)(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋白濃(nong)(nong)度(du)(du)(du)(du)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+(nmol/mg prot)=[(△A -0.0302)÷0.1475×V1)]÷(V1×Cpr)= 6.8×(△A -0.0302)÷Cpr(2)按(an)(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)鮮(xian)重(zhong)(zhong)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+(nmol/g?鮮(xian)重(zhong)(zhong))= [(△A -0.0302)÷0.1475×V1)]÷(W×V1÷V2)= 13.6×(△A -0.0302)÷W(3)按(an)(an)(an)(an)(an)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)密度(du)(du)(du)(du)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NAD+(nmol/104 cell)=[(△A -0.0302)÷0.1475×V1)]÷(500×V1÷V2)=0.027×(△A -0.0302)(二(er))NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)標(biao)(biao)(biao)準條(tiao)件(jian)下(xia)(xia)(xia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回歸曲線(xian)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?y = 0.1404x + 0.0222,R2 = 0.9976;其中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?NADH?濃(nong)(nong)度(du)(du)(du)(du)?nmol/mL1、血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)= [ (△A -0.0222)÷0.1404×V1) ]÷(V3×V1÷V2)= 142.5×(△A -0.0222)2、組(zu)(zu)織、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(1)按(an)(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋白濃(nong)(nong)度(du)(du)(du)(du)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH (nmol/mg prot)= [ (△A -0.0222)÷0.1404×V1) ]÷(V1×Cpr)= 7.1×(△A -0.0222)÷Cpr(2)按(an)(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)鮮(xian)重(zhong)(zhong)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH (nmol/g?鮮(xian)重(zhong)(zhong))=[(△A -0.0222)÷0.1404×V1) ]÷(W×V1÷V2)= 14.2×(△A -0.0222)÷W(3)按(an)(an)(an)(an)(an)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)密度(du)(du)(du)(du)計(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)NADH (nmol/104 cell)= [ (△A -0.0222)÷0.1404×V1) ]÷(500×V1÷V2)=0.028×(△A -0.0222)V1:加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)反(fan)應體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)(xi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積,0.02mL;V2:加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積,2mL;V3:加(jia)(jia)(jia)入(ru)(ru)(ru)(ru)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積:0.1mL;Cpr:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)蛋白質(zhi)(zhi)濃(nong)(nong)度(du)(du)(du)(du),mg/mL; W:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)(ben)質(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang),g;500:細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)(jun)(jun)總數(shu),500?萬。注(zhu)?意(yi):?檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?0.1nmol/mL?或(huo)(huo)(huo)(huo)?0.1nmol/g?鮮(xian)重(zhong)(zhong)?或(huo)(huo)(huo)(huo)?0.001nmol/mg prot
還(huan)(huan)原(yuan)(yuan)(yuan)(yuan)型(xing)(xing)(xing)谷(gu)胱甘肽（GSH）測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)科(ke)研實(shi)(shi)驗(yan)(yan),不(bu)(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)臨床。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)可(ke)(ke)以(yi)(yi)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing)有(you)(you)(you)(you)哪(na)(na)些???生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)驗(yan)(yan)原(yuan)(yuan)(yuan)(yuan)理(li)(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)基(ji)于(yu)化(hua)(hua)(hua)(hua)學(xue)反應(ying)(ying),理(li)(li)(li)論(lun)(lun)(lun)上(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)(bu)受(shou)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)種(zhong)屬(shu)及樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)制(zhi)(zhi)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通(tong)常(chang)(chang)(chang)只分(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing),不(bu)(bu)(bu)(bu)(bu)(bu)分(fen)(fen)(fen)(fen)種(zhong)屬(shu)。但(dan)是(shi)(shi)(shi)(shi)(shi)(shi)某(mou)(mou)些指(zhi)(zhi)(zhi)標(biao)(biao)在(zai)(zai)(zai)不(bu)(bu)(bu)(bu)(bu)(bu)同種(zhong)屬(shu)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)類(lei)(lei)(lei)型(xing)(xing)(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方式不(bu)(bu)(bu)(bu)(bu)(bu)同。因(yin)此(ci)(ci),具(ju)(ju)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)情況請(qing)參(can)見(jian)說(shuo)明(ming)(ming)書或(huo)(huo)(huo)(huo)(huo)(huo)(huo)與(yu)(yu)技(ji)術(shu)支(zhi)持聯系(xi)(xi)確(que)認(ren)(ren)(ren)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)般要(yao)(yao)(yao)求:1)于(yu)生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)言(yan),樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)無(wu)(wu)溶(rong)血(xue)(xue)(xue)、脂血(xue)(xue)(xue)、的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現(xian)象。組(zu)(zu)(zu)織(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)將血(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)洗凈擦(ca)干后(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)稱重(zhong)(zhong)(zhong)(zhong)處(chu)理(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)處(chu)理(li)(li)(li)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)盡(jin)快進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且保(bao)(bao)(bao)存(cun)(cun)(cun)于(yu)冰(bing)(bing)(bing)(bing)盒(he)(he)(he)(he)(he)之(zhi)(zhi)上(shang)(shang)(shang),待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。求3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)澄(cheng)(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),若(ruo)不(bu)(bu)(bu)(bu)(bu)(bu)澄(cheng)(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),需(xu)(xu)(xu)離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特(te)殊指(zhi)(zhi)(zhi)標(biao)(biao)除(chu)外)。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)值(zhi)(zhi)應(ying)(ying)在(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)線(xian)性(xing)(xing)(xing)(xing)范(fan)(fan)圍(wei)(wei)(wei)內,若(ruo)超(chao)過(guo)(guo)(guo)線(xian)性(xing)(xing)(xing)(xing)范(fan)(fan)圍(wei)(wei)(wei),需(xu)(xu)(xu)稀(xi)釋(shi)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若(ruo)低(di)于(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)(fan)圍(wei)(wei)(wei),需(xu)(xu)(xu)增加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)濃(nong)(nong)度(du)(du)(du)(du)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。微(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)和(he)(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)光(guang)(guang)(guang)光(guang)(guang)(guang)度(du)(du)(du)(du)法(fa)(fa)(fa)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie)?分(fen)(fen)(fen)(fen)光(guang)(guang)(guang)光(guang)(guang)(guang)度(du)(du)(du)(du)法(fa)(fa)(fa):主流(liu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)為(wei)(wei)(wei)1mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀器(qi)(qi)(qi)分(fen)(fen)(fen)(fen)光(guang)(guang)(guang)光(guang)(guang)(guang)度(du)(du)(du)(du)計(ji)(ji)(ji)(ji),能(neng)(neng)(neng)夠(gou)(gou)(gou)大(da)限(xian)(xian)度(du)(du)(du)(du)地滿足絕(jue)大(da)多(duo)數(shu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)需(xu)(xu)(xu)要(yao)(yao)(yao)。微(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa):主流(liu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)為(wei)(wei)(wei)0.2mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀器(qi)(qi)(qi)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)分(fen)(fen)(fen)(fen)光(guang)(guang)(guang)光(guang)(guang)(guang)度(du)(du)(du)(du)計(ji)(ji)(ji)(ji),測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)更加(jia)(jia)(jia)(jia)(jia)簡便(bian)快捷。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)規格(ge)(ge)中(zhong)(zhong)(zhong)(zhong)是(shi)(shi)(shi)(shi)(shi)(shi)100管(guan)(guan)(guan)(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)(he)50管(guan)(guan)(guan)(guan)(guan)(guan)是(shi)(shi)(shi)(shi)(shi)(shi)什么意(yi)(yi)(yi)思?(1)50管(guan)(guan)(guan)(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):50管(guan)(guan)(guan)(guan)(guan)(guan)指(zhi)(zhi)(zhi)能(neng)(neng)(neng)夠(gou)(gou)(gou)做(zuo)50次(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding),48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)(zhi)(zhi)可(ke)(ke)以(yi)(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)48個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)。如(ru)(ru)(ru)(ru)果(guo)(guo)(guo)(guo)每(mei)(mei)個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)重(zhong)(zhong)(zhong)(zhong)復測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)3次(ci),那么只能(neng)(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)16個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)。其中(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)(you)2次(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)空白(bai)管(guan)(guan)(guan)(guan)(guan)(guan)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)者(zhe)對(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)。值(zhi)(zhi)得特(te)別(bie)注(zhu)意(yi)(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi),50管(guan)(guan)(guan)(guan)(guan)(guan)/24樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)由(you)(you)于(yu)每(mei)(mei)個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)都(dou)(dou)需(xu)(xu)(xu)要(yao)(yao)(yao)做(zuo)對(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao),因(yin)此(ci)(ci)能(neng)(neng)(neng)夠(gou)(gou)(gou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)僅(jin)為(wei)(wei)(wei)8個(ge)(ge)(ge)(假(jia)設(she)做(zuo)3次(ci)重(zhong)(zhong)(zhong)(zhong)復)(2)100管(guan)(guan)(guan)(guan)(guan)(guan)/96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):100管(guan)(guan)(guan)(guan)(guan)(guan)指(zhi)(zhi)(zhi)能(neng)(neng)(neng)夠(gou)(gou)(gou)做(zuo)100次(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding),96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)(zhi)(zhi)可(ke)(ke)以(yi)(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)96個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin),如(ru)(ru)(ru)(ru)果(guo)(guo)(guo)(guo)每(mei)(mei)個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)重(zhong)(zhong)(zhong)(zhong)復測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)3次(ci),那么只能(neng)(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)32個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)。其中(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)(you)4次(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)空白(bai)管(guan)(guan)(guan)(guan)(guan)(guan)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)者(zhe)對(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)。注(zhu)意(yi)(yi)(yi)由(you)(you)于(yu)瓶(ping)(ping)璧(bi)和(he)(he)(he)(he)(he)(he)(he)(he)移液(ye)(ye)(ye)(ye)(ye)(ye)器(qi)(qi)(qi)吸(xi)(xi)附等(deng),通(tong)常(chang)(chang)(chang)要(yao)(yao)(yao)比(bi)(bi)(bi)(bi)(bi)(bi)(bi)理(li)(li)(li)論(lun)(lun)(lun)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)次(ci)數(shu)少(shao)1~3次(ci)。其它規格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)義依(yi)次(ci)類(lei)(lei)(lei)推。值(zhi)(zhi)得特(te)別(bie)注(zhu)意(yi)(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi),100管(guan)(guan)(guan)(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)由(you)(you)于(yu)每(mei)(mei)個(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)都(dou)(dou)需(xu)(xu)(xu)要(yao)(yao)(yao)做(zuo)對(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao),因(yin)此(ci)(ci)能(neng)(neng)(neng)夠(gou)(gou)(gou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)僅(jin)為(wei)(wei)(wei)16個(ge)(ge)(ge)(假(jia)設(she)做(zuo)3次(ci)重(zhong)(zhong)(zhong)(zhong)復)。如(ru)(ru)(ru)(ru)何防(fang)(fang)止(zhi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)失效(xiao)?????不(bu)(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)過(guo)(guo)(guo)早訂購,以(yi)(yi)免試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)失效(xiao).收到(dao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)后(hou)(hou)(hou)(hou)(hou)(hou)(hou),檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)查外包裝是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)(fou)正(zheng)(zheng)常(chang)(chang)(chang)。馬上(shang)(shang)(shang)打開,按(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)說(shuo)明(ming)(ming)書核對(dui)(dui)(dui)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)(fou)正(zheng)(zheng)確(que),注(zhu)意(yi)(yi)(yi)瓶(ping)(ping)中(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)狀態(tai)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)(fou)與(yu)(yu)說(shuo)明(ming)(ming)書一(yi)(yi)(yi)致(zhi)。如(ru)(ru)(ru)(ru)果(guo)(guo)(guo)(guo)有(you)(you)(you)(you)問(wen)題,或(huo)(huo)(huo)(huo)(huo)(huo)(huo)者(zhe)懷疑,馬上(shang)(shang)(shang)聯系(xi)(xi)公(gong)司(si)銷(xiao)(xiao)售(shou),進(jin)(jin)(jin)(jin)行(xing)(xing)確(que)認(ren)(ren)(ren)。如(ru)(ru)(ru)(ru)果(guo)(guo)(guo)(guo)沒(mei)有(you)(you)(you)(you)問(wen)題,請(qing)務(wu)(wu)必(bi)按(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)說(shuo)明(ming)(ming)書進(jin)(jin)(jin)(jin)行(xing)(xing)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)(bao)存(cun)(cun)(cun)。注(zhu)意(yi)(yi)(yi),不(bu)(bu)(bu)(bu)(bu)(bu)同試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji),可(ke)(ke)能(neng)(neng)(neng)需(xu)(xu)(xu)要(yao)(yao)(yao)分(fen)(fen)(fen)(fen)別(bie)保(bao)(bao)(bao)存(cun)(cun)(cun)在(zai)(zai)(zai)常(chang)(chang)(chang)溫(25°℃)、4℃和(he)(he)(he)(he)(he)(he)(he)(he)-20℃,務(wu)(wu)必(bi)分(fen)(fen)(fen)(fen)別(bie)按(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)要(yao)(yao)(yao)求保(bao)(bao)(bao)存(cun)(cun)(cun),不(bu)(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)整(zheng)(zheng)個(ge)(ge)(ge)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)放(fang)置(zhi)于(yu)冰(bing)(bing)(bing)(bing)箱。如(ru)(ru)(ru)(ru)何進(jin)(jin)(jin)(jin)行(xing)(xing)預(yu)(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)?(1)務(wu)(wu)必(bi)在(zai)(zai)(zai)7個(ge)(ge)(ge)工作(zuo)(zuo)日內進(jin)(jin)(jin)(jin)行(xing)(xing)預(yu)(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding),以(yi)(yi)確(que)定(ding)(ding)(ding)(ding)(ding)該試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)(fou)適合(he)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin),以(yi)(yi)免造成(cheng)(cheng)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)和(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浪費(fei)。(2)選(xuan)(xuan)擇(ze)2~3個(ge)(ge)(ge)預(yu)(yu)(yu)期差異比(bi)(bi)(bi)(bi)(bi)(bi)(bi)較(jiao)大(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin),嚴格(ge)(ge)按(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)說(shuo)明(ming)(ming)書進(jin)(jin)(jin)(jin)行(xing)(xing)操(cao)作(zuo)(zuo),注(zhu)意(yi)(yi)(yi)儀器(qi)(qi)(qi)工作(zuo)(zuo)狀態(tai),操(cao)作(zuo)(zuo)規范(fan)(fan),控(kong)制(zhi)(zhi)好測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)條件,尤其是(shi)(shi)(shi)(shi)(shi)(shi)溫度(du)(du)(du)(du)。(3)如(ru)(ru)(ru)(ru)實(shi)(shi)與(yu)(yu)公(gong)司(si)銷(xiao)(xiao)售(shou)溝(gou)通(tong)預(yu)(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)結(jie)果(guo)(guo)(guo)(guo),公(gong)司(si)技(ji)術(shu)人員會(hui)確(que)認(ren)(ren)(ren)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)結(jie)果(guo)(guo)(guo)(guo)是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)(fou)正(zheng)(zheng)常(chang)(chang)(chang),是(shi)(shi)(shi)(shi)(shi)(shi)否(fou)(fou)(fou)需(xu)(xu)(xu)要(yao)(yao)(yao)調整(zheng)(zheng),如(ru)(ru)(ru)(ru)何進(jin)(jin)(jin)(jin)行(xing)(xing)調整(zheng)(zheng),從(cong)(cong)(cong)(cong)而(er)確(que)保(bao)(bao)(bao)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)結(jie)果(guo)(guo)(guo)(guo)可(ke)(ke)靠。比(bi)(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)皿(min)(min)有(you)(you)(you)(you)哪(na)(na)些規格(ge)(ge)?(1)常(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)比(bi)(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)皿(min)(min),如(ru)(ru)(ru)(ru)1mL和(he)(he)(he)(he)(he)(he)(he)(he)3mL等(deng),常(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)都(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)選(xuan)(xuan)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)1mL體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi),只能(neng)(neng)(neng)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)1mL比(bi)(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)皿(min)(min),不(bu)(bu)(bu)(bu)(bu)(bu)可(ke)(ke)以(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)更大(da)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)皿(min)(min),否(fou)(fou)(fou)則(ze)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)(bu)(bu)夠(gou)(gou)(gou),無(wu)(wu)法(fa)(fa)(fa)正(zheng)(zheng)常(chang)(chang)(chang)比(bi)(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)。(2)微(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)比(bi)(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)皿(min)(min),如(ru)(ru)(ru)(ru)0.25mL和(he)(he)(he)(he)(he)(he)(he)(he)0.5mL等(deng),微(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)都(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)選(xuan)(xuan)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)0.25mL比(bi)(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)皿(min)(min)。一(yi)(yi)(yi)般客戶(hu)都(dou)(dou)選(xuan)(xuan)擇(ze)使用(yong)(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)儀(需(xu)(xu)(xu)要(yao)(yao)(yao)自備酶(mei)(mei)標(biao)(biao)板)(3)紫外波長檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)需(xu)(xu)(xu)要(yao)(yao)(yao)石英(ying)比(bi)(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)皿(min)(min),不(bu)(bu)(bu)(bu)(bu)(bu)可(ke)(ke)以(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)普通(tong)玻璃比(bi)(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)皿(min)(min)。(4)不(bu)(bu)(bu)(bu)(bu)(bu)同規格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)皿(min)(min),其外觀大(da)小是(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)(yi)致(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de),都(dou)(dou)是(shi)(shi)(shi)(shi)(shi)(shi)1cm光(guang)(guang)(guang)徑(也(ye)(ye)有(you)(you)(you)(you)0.5cm光(guang)(guang)(guang)徑的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)皿(min)(min),不(bu)(bu)(bu)(bu)(bu)(bu)常(chang)(chang)(chang)見(jian),也(ye)(ye)可(ke)(ke)以(yi)(yi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong),但(dan)是(shi)(shi)(shi)(shi)(shi)(shi)計(ji)(ji)(ji)(ji)算(suan)(suan)時(shi)(shi)要(yao)(yao)(yao)注(zhu)意(yi)(yi)(yi)修改(gai)計(ji)(ji)(ji)(ji)算(suan)(suan)公(gong)式),其內容(rong)積(ji)差異在(zai)(zai)(zai)于(yu)壁的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)厚度(du)(du)(du)(du)。代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)優(you)點(1)更加(jia)(jia)(jia)(jia)(jia)省事兒,完(wan)(wan)(wan)全(quan)不(bu)(bu)(bu)(bu)(bu)(bu)必(bi)為(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)犯難(nan)。用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)只要(yao)(yao)(yao)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)供(gong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)和(he)(he)(he)(he)(he)(he)(he)(he)想測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)指(zhi)(zhi)(zhi)標(biao)(biao),就不(bu)(bu)(bu)(bu)(bu)(bu)再(zai)需(xu)(xu)(xu)要(yao)(yao)(yao)為(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)操(cao)心(xin)(xin)(xin)(xin)了,不(bu)(bu)(bu)(bu)(bu)(bu)再(zai)受(shou)到(dao)操(cao)作(zuo)(zuo)技(ji)能(neng)(neng)(neng)、儀器(qi)(qi)(qi)設(she)備和(he)(he)(he)(he)(he)(he)(he)(he)時(shi)(shi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)制(zhi)(zhi),就等(deng)著拿到(dao)數(shu)據(ju)(ju)寫論(lun)(lun)(lun)文(wen)。(2)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)數(shu)據(ju)(ju)更有(you)(you)(you)(you)保(bao)(bao)(bao)障。嚴格(ge)(ge)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)質(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)管(guan)(guan)(guan)(guan)(guan)(guan)理(li)(li)(li)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi),從(cong)(cong)(cong)(cong)而(er)保(bao)(bao)(bao)證了測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)結(jie)果(guo)(guo)(guo)(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)真(zhen)實(shi)(shi)可(ke)(ke)靠。我們還(huan)(huan)建(jian)(jian)立了免費(fei)預(yu)(yu)(yu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)制(zhi)(zhi)度(du)(du)(du)(du)。(3)防(fang)(fang)止(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)損(sun)(sun)失無(wu)(wu)論(lun)(lun)(lun)是(shi)(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)自己完(wan)(wan)(wan)成(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)全(quan)過(guo)(guo)(guo)程(cheng),還(huan)(huan)是(shi)(shi)(shi)(shi)(shi)(shi)購買試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)完(wan)(wan)(wan)成(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding),都(dou)(dou)可(ke)(ke)能(neng)(neng)(neng)因(yin)為(wei)(wei)(wei)缺乏(fa)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)經驗(yan)(yan),導致(zhi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)失敗(bai),從(cong)(cong)(cong)(cong)而(er)損(sun)(sun)失樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情況。對(dui)(dui)(dui)于(yu)生(sheng)(sheng)(sheng)命科(ke)學(xue)研究(jiu)而(er)言(yan),樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)是(shi)(shi)(shi)(shi)(shi)(shi)＊珍貴(gui)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)一(yi)(yi)(yi)旦(dan)損(sun)(sun)失,輕則(ze)造成(cheng)(cheng)研究(jiu)進(jin)(jin)(jin)(jin)度(du)(du)(du)(du)被(bei)(bei)耽擱(ge),重(zhong)(zhong)(zhong)(zhong)則(ze)造成(cheng)(cheng)研究(jiu)完(wan)(wan)(wan)全(quan)失敗(bai)!代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)能(neng)(neng)(neng)夠(gou)(gou)(gou)充分(fen)(fen)(fen)(fen)保(bao)(bao)(bao)證測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)功(gong),從(cong)(cong)(cong)(cong)而(er)防(fang)(fang)止(zhi)了樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)損(sun)(sun)失。當然,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)質(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)功(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti),而(er)且樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)質(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)只有(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)自己才能(neng)(neng)(neng)控(kong)制(zhi)(zhi)和(he)(he)(he)(he)(he)(he)(he)(he)保(bao)(bao)(bao)證。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)本(ben)(ben)(ben)(ben)(ben)原(yuan)(yuan)(yuan)(yuan)理(li)(li)(li)??生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he),又(you)稱生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he),是(shi)(shi)(shi)(shi)(shi)(shi)利用(yong)(yong)(yong)(yong)(yong)(yong)(yong)特(te)定(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)成(cheng)(cheng)分(fen)(fen)(fen)(fen)、動(dong)力(li)學(xue)及化(hua)(hua)(hua)(hua)學(xue)反應(ying)(ying)等(deng)技(ji)術(shu)制(zhi)(zhi)備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he),用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)生(sheng)(sheng)(sheng)理(li)(li)(li)學(xue)、病(bing)理(li)(li)(li)學(xue)、藥理(li)(li)(li)學(xue)等(deng)領(ling)域中(zhong)(zhong)(zhong)(zhong)特(te)定(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)分(fen)(fen)(fen)(fen)子(zi)(zi)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)化(hua)(hua)(hua)(hua)學(xue)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)包括底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、酶(mei)(mei)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)、探(tan)針等(deng)多(duo)個(ge)(ge)(ge)組(zu)(zu)(zu)成(cheng)(cheng)部(bu)分(fen)(fen)(fen)(fen),其中(zhong)(zhong)(zhong)(zhong)＊常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)法(fa)(fa)(fa)。底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)法(fa)(fa)(fa)是(shi)(shi)(shi)(shi)(shi)(shi)生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)本(ben)(ben)(ben)(ben)(ben)原(yuan)(yuan)(yuan)(yuan)理(li)(li)(li)。在(zai)(zai)(zai)該過(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong),底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)與(yu)(yu)酶(mei)(mei)在(zai)(zai)(zai)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)膜上(shang)(shang)(shang)結(jie)合(he)形成(cheng)(cheng)基(ji)質(zhi)酶(mei)(mei),進(jin)(jin)(jin)(jin)而(er)形成(cheng)(cheng)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)復合(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)。隨著時(shi)(shi)間(jian)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)推移,底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)(mei)復合(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)逐漸分(fen)(fen)(fen)(fen)解(jie),釋(shi)放(fang)出(chu)分(fen)(fen)(fen)(fen)子(zi)(zi)小的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)子(zi)(zi)大(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)未完(wan)(wan)(wan)全(quan)消化(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)。未完(wan)(wan)(wan)全(quan)消化(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)可(ke)(ke)以(yi)(yi)被(bei)(bei)一(yi)(yi)(yi)系(xi)(xi)列(lie)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)(mei)代(dai)謝(xie)(xie),同時(shi)(shi)底(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、酶(mei)(mei)和(he)(he)(he)(he)(he)(he)(he)(he)代(dai)謝(xie)(xie)產(chan)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)都(dou)(dou)會(hui)對(dui)(dui)(dui)復合(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)穩定(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)產(chan)生(sheng)(sheng)(sheng)影響(xiang),從(cong)(cong)(cong)(cong)而(er)影響(xiang)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結(jie)果(guo)(guo)(guo)(guo)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)可(ke)(ke)以(yi)(yi)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing)有(you)(you)(you)(you)哪(na)(na)些?? ?生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)驗(yan)(yan)原(yuan)(yuan)(yuan)(yuan)理(li)(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)基(ji)于(yu)化(hua)(hua)(hua)(hua)學(xue)反應(ying)(ying),理(li)(li)(li)論(lun)(lun)(lun)上(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)(bu)受(shou)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)種(zhong)屬(shu)及樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限(xian)(xian)制(zhi)(zhi)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通(tong)常(chang)(chang)(chang)只分(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing),不(bu)(bu)(bu)(bu)(bu)(bu)分(fen)(fen)(fen)(fen)種(zhong)屬(shu)。但(dan)是(shi)(shi)(shi)(shi)(shi)(shi)某(mou)(mou)些指(zhi)(zhi)(zhi)標(biao)(biao)在(zai)(zai)(zai)不(bu)(bu)(bu)(bu)(bu)(bu)同種(zhong)屬(shu)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)類(lei)(lei)(lei)型(xing)(xing)(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方式不(bu)(bu)(bu)(bu)(bu)(bu)同。因(yin)此(ci)(ci),具(ju)(ju)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)情況請(qing)參(can)見(jian)說(shuo)明(ming)(ming)書或(huo)(huo)(huo)(huo)(huo)(huo)(huo)與(yu)(yu)技(ji)術(shu)支(zhi)持聯系(xi)(xi)確(que)認(ren)(ren)(ren)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)般要(yao)(yao)(yao)求:1)于(yu)生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)言(yan),樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)＊-好無(wu)(wu)溶(rong)血(xue)(xue)(xue)、脂血(xue)(xue)(xue)、黃-疸(dan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現(xian)象。組(zu)(zu)(zu)織(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)將血(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)洗凈擦(ca)干后(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)稱重(zhong)(zhong)(zhong)(zhong)處(chu)理(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)處(chu)理(li)(li)(li)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)＊-好盡(jin)快進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且保(bao)(bao)(bao)存(cun)(cun)(cun)于(yu)冰(bing)(bing)(bing)(bing)盒(he)(he)(he)(he)(he)之(zhi)(zhi)上(shang)(shang)(shang),待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)澄(cheng)(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),若(ruo)不(bu)(bu)(bu)(bu)(bu)(bu)澄(cheng)(cheng)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),需(xu)(xu)(xu)離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特(te)殊指(zhi)(zhi)(zhi)標(biao)(biao)除(chu)外)。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)值(zhi)(zhi)應(ying)(ying)在(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)線(xian)性(xing)(xing)(xing)(xing)范(fan)(fan)圍(wei)(wei)(wei)內,若(ruo)超(chao)過(guo)(guo)(guo)線(xian)性(xing)(xing)(xing)(xing)范(fan)(fan)圍(wei)(wei)(wei),需(xu)(xu)(xu)稀(xi)釋(shi)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若(ruo)低(di)于(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)(fan)圍(wei)(wei)(wei),需(xu)(xu)(xu)增加(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)濃(nong)(nong)度(du)(du)(du)(du)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)進(jin)(jin)(jin)(jin)行(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。還(huan)(huan)原(yuan)(yuan)(yuan)(yuan)型(xing)(xing)(xing)谷(gu)胱甘肽（GSH）測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)科(ke)研實(shi)(shi)驗(yan)(yan),不(bu)(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)臨床。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)意(yi)(yi)(yi)義:輔酶(mei)(mei)NAD(H)廣泛存(cun)(cun)(cun)在(zai)(zai)(zai)于(yu)動(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、植物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、微(wei)(wei)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)(he)培養細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong),NAD+是(shi)(shi)(shi)(shi)(shi)(shi)糖酵解(jie)(EMP)和(he)(he)(he)(he)(he)(he)(he)(he)三(san)羧(suo)酸(suan)(suan)循環(huan)(TCA)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)(yao)(yao)氫(qing)受(shou)體(ti)(ti)(ti)(ti)(ti)(ti)(ti),生(sheng)(sheng)(sheng)成(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?NADH?經呼(hu)吸(xi)(xi)電子(zi)(zi)鏈(ETC)傳遞把電子(zi)(zi)交給氧(yang),在(zai)(zai)(zai)合(he)成(cheng)(cheng)?ATP?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同時(shi)(shi),形成(cheng)(cheng)大(da)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?ROS,同時(shi)(shi)?NADH?再(zai)生(sheng)(sheng)(sheng)為(wei)(wei)(wei)?NAD+。糖、脂、蛋(dan)白(bai)質(zhi)三(san)大(da)代(dai)謝(xie)(xie)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)分(fen)(fen)(fen)(fen)解(jie)中(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)氧(yang)化(hua)(hua)(hua)(hua)反應(ying)(ying)絕(jue)大(da)部(bu)分(fen)(fen)(fen)(fen)通(tong)過(guo)(guo)(guo)這(zhe)一(yi)(yi)(yi)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)完(wan)(wan)(wan)成(cheng)(cheng)。NAD(H)含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)(he)(he)(he)?NADH/NAD+比(bi)(bi)(bi)(bi)(bi)(bi)(bi)值(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)高(gao)低(di)可(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)于(yu)評(ping)價糖酵解(jie)和(he)(he)(he)(he)(he)(he)(he)(he)?TCA?循環(huan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)強弱。較(jiao)高(gao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)NAD(H)及?NADH/NAD+比(bi)(bi)(bi)(bi)(bi)(bi)(bi)值(zhi)(zhi)說(shuo)明(ming)(ming)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)呼(hu)吸(xi)(xi)耗氧(yang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)較(jiao)高(gao),處(chu)于(yu)過(guo)(guo)(guo)氧(yang)化(hua)(hua)(hua)(hua)狀態(tai)。此(ci)(ci)外,NADH/NAD+比(bi)(bi)(bi)(bi)(bi)(bi)(bi)值(zhi)(zhi)升高(gao)也(ye)(ye)可(ke)(ke)抑制(zhi)(zhi)糖酵解(jie)和(he)(he)(he)(he)(he)(he)(he)(he)?TCA?循環(huan)。另外,NAD+降解(jie)產(chan)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)對(dui)(dui)(dui)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)信(xin)號傳導、代(dai)謝(xie)(xie)和(he)(he)(he)(he)(he)(he)(he)(he)基(ji)因(yin)表(biao)達等(deng)具(ju)(ju)有(you)(you)(you)(you)重(zhong)(zhong)(zhong)(zhong)要(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)調控(kong)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)原(yuan)(yuan)(yuan)(yuan)理(li)(li)(li):分(fen)(fen)(fen)(fen)別(bie)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)酸(suan)(suan)性(xing)(xing)(xing)(xing)和(he)(he)(he)(he)(he)(he)(he)(he)堿(jian)(jian)(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)?NADH,NADH?通(tong)過(guo)(guo)(guo)?PMS?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)遞氫(qing)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)(yong),還(huan)(huan)原(yuan)(yuan)(yuan)(yuan)氧(yang)化(hua)(hua)(hua)(hua)型(xing)(xing)(xing)噻唑(zuo)藍(MTT)為(wei)(wei)(wei)甲瓚,在(zai)(zai)(zai)?570nm?下(xia)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)吸(xi)(xi)光(guang)(guang)(guang)值(zhi)(zhi);而(er)?NAD+可(ke)(ke)被(bei)(bei)乙醇脫氫(qing)酶(mei)(mei)還(huan)(huan)原(yuan)(yuan)(yuan)(yuan)為(wei)(wei)(wei)?NADH,進(jin)(jin)(jin)(jin)一(yi)(yi)(yi)步(bu)采用(yong)(yong)(yong)(yong)(yong)(yong)(yong)?MTT?還(huan)(huan)原(yuan)(yuan)(yuan)(yuan)法(fa)(fa)(fa)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。需(xu)(xu)(xu)自備的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)儀器(qi)(qi)(qi)和(he)(he)(he)(he)(he)(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)(yong)品(pin)(pin)(pin)(pin)(pin):酶(mei)(mei)標(biao)(biao)儀、臺式離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)機、移液(ye)(ye)(ye)(ye)(ye)(ye)器(qi)(qi)(qi)、96?孔板、研缽、冰(bing)(bing)(bing)(bing)和(he)(he)(he)(he)(he)(he)(he)(he)蒸餾水(shui)(shui)。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)(zu)成(cheng)(cheng)和(he)(he)(he)(he)(he)(he)(he)(he)配制(zhi)(zhi):酸(suan)(suan)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping),4℃保(bao)(bao)(bao)存(cun)(cun)(cun);堿(jian)(jian)(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping),4℃保(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi):液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?10 mL×1?瓶(ping)(ping),4℃保(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)二(er):液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?3 mL×1?瓶(ping)(ping),4℃保(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)三(san):粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping)(ping),-20?℃保(bao)(bao)(bao)存(cun)(cun)(cun),用(yong)(yong)(yong)(yong)(yong)(yong)(yong)時(shi)(shi)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)?3mL?蒸餾水(shui)(shui),混(hun)(hun)勻(yun)(yun)(yun),用(yong)(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)(bu)完(wan)(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)存(cun)(cun)(cun)一(yi)(yi)(yi)周(zhou);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)四(si):粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping)(ping),4?℃保(bao)(bao)(bao)存(cun)(cun)(cun),用(yong)(yong)(yong)(yong)(yong)(yong)(yong)時(shi)(shi)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)?3mL?蒸餾水(shui)(shui),混(hun)(hun)勻(yun)(yun)(yun),用(yong)(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)(bu)完(wan)(wan)(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)存(cun)(cun)(cun)一(yi)(yi)(yi)周(zhou);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)五:液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?3.6mL×1?瓶(ping)(ping),4?℃保(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六:液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?30mL×1?瓶(ping)(ping),4?℃保(bao)(bao)(bao)存(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)七:液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping),4?℃保(bao)(bao)(bao)存(cun)(cun)(cun)。NAD+和(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):1?血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang))中(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL):酸(suan)(suan)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)(bi)例(建(jian)(jian)議(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1mL?血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)),加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)?1mL?酸(suan)(suan)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)浴(yu)?5min(蓋緊(jin)(jin)(jin),以(yi)(yi)防(fang)(fang)止(zhi)水(shui)(shui)分(fen)(fen)(fen)(fen)散失);冰(bing)(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)冷卻(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun)(yun),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL):堿(jian)(jian)(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)(bi)例(建(jian)(jian)議(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1mL血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)),加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)?1mL?堿(jian)(jian)(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)浴(yu)?5min(蓋緊(jin)(jin)(jin),以(yi)(yi)防(fang)(fang)止(zhi)水(shui)(shui)分(fen)(fen)(fen)(fen)散失);冰(bing)(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)冷卻(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)?500μL?酸(suan)(suan)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun)(yun),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。2?組(zu)(zu)(zu)織(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)組(zu)(zu)(zu)織(zhi)(zhi)(zhi)質(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(g):酸(suan)(suan)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)(bi)例(建(jian)(jian)議(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1g?組(zu)(zu)(zu)織(zhi)(zhi)(zhi),加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)?1mL酸(suan)(suan)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)浴(yu)研磨,95℃水(shui)(shui)浴(yu)?5min(蓋緊(jin)(jin)(jin),以(yi)(yi)防(fang)(fang)止(zhi)水(shui)(shui)分(fen)(fen)(fen)(fen)散失);冰(bing)(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)冷卻(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun)(yun),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)組(zu)(zu)(zu)織(zhi)(zhi)(zhi)質(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(g):堿(jian)(jian)(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)(bi)例(建(jian)(jian)議(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約?0.1g?組(zu)(zu)(zu)織(zhi)(zhi)(zhi),加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)?1mL堿(jian)(jian)(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),冰(bing)(bing)(bing)(bing)浴(yu)研磨,95℃水(shui)(shui)浴(yu)?5min(蓋緊(jin)(jin)(jin),以(yi)(yi)防(fang)(fang)止(zhi)水(shui)(shui)分(fen)(fen)(fen)(fen)散失);冰(bing)(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)冷卻(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)?500μL?酸(suan)(suan)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun)(yun),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3?細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)中(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先收集(ji)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)到(dao)離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)管(guan)(guan)(guan)(guan)(guan)(guan)內,棄上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),按(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)):酸(suan)(suan)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)(bi)例(建(jian)(jian)議(yi)?500?萬(wan)(wan)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)?1mL?酸(suan)(suan)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),超(chao)聲波破碎(sui)(冰(bing)(bing)(bing)(bing)浴(yu),功(gong)率(lv)20%或(huo)(huo)(huo)(huo)(huo)(huo)(huo)?200W,超(chao)聲?3s,間(jian)隔?10s,重(zhong)(zhong)(zhong)(zhong)復?30?次(ci)),95℃水(shui)(shui)浴(yu)?5min(蓋緊(jin)(jin)(jin),以(yi)(yi)防(fang)(fang)止(zhi)水(shui)(shui)分(fen)(fen)(fen)(fen)散失);冰(bing)(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)冷卻(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)?500μL?堿(jian)(jian)(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun)(yun),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先收集(ji)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)到(dao)離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)管(guan)(guan)(guan)(guan)(guan)(guan)內,棄上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),按(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)數(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)):堿(jian)(jian)(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(mL)為(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)(bi)(bi)例(建(jian)(jian)議(yi)?500?萬(wan)(wan)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)?1mL?堿(jian)(jian)(jian)(jian)(jian)(jian)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)),超(chao)聲波破碎(sui)(冰(bing)(bing)(bing)(bing)浴(yu),功(gong)率(lv)20%或(huo)(huo)(huo)(huo)(huo)(huo)(huo)?200W,超(chao)聲?3s,間(jian)隔?10s,重(zhong)(zhong)(zhong)(zhong)復?30?次(ci)),95℃水(shui)(shui)浴(yu)?5min(蓋緊(jin)(jin)(jin),以(yi)(yi)防(fang)(fang)止(zhi)水(shui)(shui)分(fen)(fen)(fen)(fen)散失);冰(bing)(bing)(bing)(bing)浴(yu)中(zhong)(zhong)(zhong)(zhong)冷卻(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)?500μL?酸(suan)(suan)性(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)使之(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he),混(hun)(hun)勻(yun)(yun)(yun),10000g 4?℃離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)冰(bing)(bing)(bing)(bing)上(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。充分(fen)(fen)(fen)(fen)混(hun)(hun)勻(yun)(yun)(yun),靜置(zhi)?5min?后(hou)(hou)(hou)(hou)(hou)(hou)(hou),20000g,25℃離(li)(li)(li)(li)心(xin)(xin)(xin)(xin)?5min,棄上(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)(qing),沉(chen)淀中(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru):混(hun)(hun)勻(yun)(yun)(yun),取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?200μL?轉移至微(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)石英(ying)比(bi)(bi)(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)皿(min)(min)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)?96?孔板中(zhong)(zhong)(zhong)(zhong),570nm?下(xia)讀取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)對(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)吸(xi)(xi)光(guang)(guang)(guang)值(zhi)(zhi)?A1?和(he)(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)吸(xi)(xi)光(guang)(guang)(guang)值(zhi)(zhi)?A2,計(ji)(ji)(ji)(ji)算(suan)(suan)△A=A2-A1。注(zhu)意(yi)(yi)(yi)事項:1、如(ru)(ru)(ru)(ru)果(guo)(guo)(guo)(guo)一(yi)(yi)(yi)次(ci)性(xing)(xing)(xing)(xing)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)數(shu)較(jiao)多(duo),可(ke)(ke)將試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)、二(er)、三(san)和(he)(he)(he)(he)(he)(he)(he)(he)四(si)按(an)(an)(an)(an)(an)(an)比(bi)(bi)(bi)(bi)(bi)(bi)(bi)例配成(cheng)(cheng)混(hun)(hun)合(he)液(ye)(ye)(ye)(ye)(ye)(ye)。2、對(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)步(bu)驟的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie):對(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan)加(jia)(jia)(jia)(jia)(jia)完(wan)(wan)(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)、二(er)、三(san)、四(si)和(he)(he)(he)(he)(he)(he)(he)(he)五后(hou)(hou)(hou)(hou)(hou)(hou)(hou)必(bi)須馬上(shang)(shang)(shang)加(jia)(jia)(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六;測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)加(jia)(jia)(jia)(jia)(jia)完(wan)(wan)(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)、二(er)、三(san)、四(si)和(he)(he)(he)(he)(he)(he)(he)(he)五后(hou)(hou)(hou)(hou)(hou)(hou)(hou)必(bi)須反應(ying)(ying)?20min?后(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)加(jia)(jia)(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六。3、反應(ying)(ying)過(guo)(guo)(guo)程(cheng)中(zhong)(zhong)(zhong)(zhong)注(zhu)意(yi)(yi)(yi)避光(guang)(guang)(guang)。4、若(ruo)?NAD+測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0302,NADH?測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0222,說(shuo)明(ming)(ming)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)輔酶(mei)(mei)含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)較(jiao)低(di),已低(di)于(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian)(xian),可(ke)(ke)做(zuo)如(ru)(ru)(ru)(ru)下(xia)調整(zheng)(zheng):(1)將測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)避光(guang)(guang)(guang)靜置(zhi)時(shi)(shi)間(jian)?20min?延長到(dao)?60min;(2)在(zai)(zai)(zai)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)階段增加(jia)(jia)(jia)(jia)(jia)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang),即取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?0.2g?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)?0.2mL?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)?1mL?提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)。5、由(you)(you)于(yu)每(mei)(mei)一(yi)(yi)(yi)個(ge)(ge)(ge)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)(guan)(guan)需(xu)(xu)(xu)要(yao)(yao)(yao)設(she)一(yi)(yi)(yi)個(ge)(ge)(ge)對(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)(guan)(guan),本(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)?100?管(guan)(guan)(guan)(guan)(guan)(guan)保(bao)(bao)(bao)證測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)?48?個(ge)(ge)(ge)?NAD+或(huo)(huo)(huo)(huo)(huo)(huo)(huo)?NADH.NAD+和(he)(he)(he)(he)(he)(he)(he)(he)?NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)算(suan)(suan):(一(yi)(yi)(yi)) NAD+含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)算(suan)(suan)標(biao)(biao)準條件下(xia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回(hui)歸(gui)曲線(xian)為(wei)(wei)(wei)?y = 0.1475x + 0.0302,R2 = 0.9978;其中(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)?NAD+濃(nong)(nong)度(du)(du)(du)(du)?nmol/mL1、血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang))中(zhong)(zhong)(zhong)(zhong)?NAD+含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)算(suan)(suan)NAD+含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)=[(△A -0.0302)÷0.1475×V1) ]÷(V3×V1÷V2)=135.6×(△A -0.0302)2、組(zu)(zu)(zu)織(zhi)(zhi)(zhi)、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)?NAD+含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)算(suan)(suan)(1)按(an)(an)(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)蛋(dan)白(bai)濃(nong)(nong)度(du)(du)(du)(du)計(ji)(ji)(ji)(ji)算(suan)(suan)NAD+(nmol/mg prot)=[(△A -0.0302)÷0.1475×V1)]÷(V1×Cpr)= 6.8×(△A -0.0302)÷Cpr(2)按(an)(an)(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)鮮重(zhong)(zhong)(zhong)(zhong)計(ji)(ji)(ji)(ji)算(suan)(suan)NAD+(nmol/g?鮮重(zhong)(zhong)(zhong)(zhong))= [(△A -0.0302)÷0.1475×V1)]÷(W×V1÷V2)= 13.6×(△A -0.0302)÷W(3)按(an)(an)(an)(an)(an)(an)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)密(mi)度(du)(du)(du)(du)計(ji)(ji)(ji)(ji)算(suan)(suan)NAD+(nmol/104 cell)=[(△A -0.0302)÷0.1475×V1)]÷(500×V1÷V2)=0.027×(△A -0.0302)(二(er))NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)算(suan)(suan)標(biao)(biao)準條件下(xia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回(hui)歸(gui)曲線(xian)為(wei)(wei)(wei)?y = 0.1404x + 0.0222,R2 = 0.9976;其中(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)?NADH?濃(nong)(nong)度(du)(du)(du)(du)?nmol/mL1、血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang))中(zhong)(zhong)(zhong)(zhong)?NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)算(suan)(suan)NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)= [ (△A -0.0222)÷0.1404×V1) ]÷(V3×V1÷V2)= 142.5×(△A -0.0222)2、組(zu)(zu)(zu)織(zhi)(zhi)(zhi)、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)中(zhong)(zhong)(zhong)(zhong)?NADH?含(han)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)算(suan)(suan)(1)按(an)(an)(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)蛋(dan)白(bai)濃(nong)(nong)度(du)(du)(du)(du)計(ji)(ji)(ji)(ji)算(suan)(suan)NADH (nmol/mg prot)= [ (△A -0.0222)÷0.1404×V1) ]÷(V1×Cpr)= 7.1×(△A -0.0222)÷Cpr(2)按(an)(an)(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)鮮重(zhong)(zhong)(zhong)(zhong)計(ji)(ji)(ji)(ji)算(suan)(suan)NADH (nmol/g?鮮重(zhong)(zhong)(zhong)(zhong))=[(△A -0.0222)÷0.1404×V1) ]÷(W×V1÷V2)= 14.2×(△A -0.0222)÷W(3)按(an)(an)(an)(an)(an)(an)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)密(mi)度(du)(du)(du)(du)計(ji)(ji)(ji)(ji)算(suan)(suan)NADH (nmol/104 cell)= [ (△A -0.0222)÷0.1404×V1) ]÷(500×V1÷V2)=0.028×(△A -0.0222)V1:加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)反應(ying)(ying)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)中(zhong)(zhong)(zhong)(zhong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji),0.02mL;V2:加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)提(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji),2mL;V3:加(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)(ru)(ru)(ru)(ru)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji):0.1mL;Cpr:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)蛋(dan)白(bai)質(zhi)濃(nong)(nong)度(du)(du)(du)(du),mg/mL; W:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)質(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang)(liang),g;500:細(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)總數(shu),500?萬(wan)(wan)。注(zhu)?意(yi)(yi)(yi):?檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限(xian)(xian)為(wei)(wei)(wei)?0.1nmol/mL?或(huo)(huo)(huo)(huo)(huo)(huo)(huo)?0.1nmol/g?鮮重(zhong)(zhong)(zhong)(zhong)?或(huo)(huo)(huo)(huo)(huo)(huo)(huo)?0.001nmol/mg prot
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