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ATP含(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)科研(yan)(yan)實(shi)(shi)驗,不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)臨(lin)床。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)可(ke)(ke)(ke)(ke)以(yi)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing)有(you)(you)(you)(you)(you)(you)哪些(xie)(xie)???生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)驗原(yuan)理(li)(li)(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)基(ji)于(yu)(yu)(yu)(yu)(yu)(yu)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)反(fan)(fan)(fan)應(ying)(ying)(ying),理(li)(li)(li)(li)論(lun)(lun)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)受(shou)(shou)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)種(zhong)屬(shu)(shu)及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限制(zhi)(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通(tong)常(chang)(chang)(chang)(chang)(chang)(chang)只(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)種(zhong)屬(shu)(shu)。但(dan)(dan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)某(mou)(mou)些(xie)(xie)指(zhi)(zhi)標(biao)(biao)(biao)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)種(zhong)屬(shu)(shu)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)類(lei)(lei)(lei)型(xing)(xing)(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方(fang)(fang)式不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)。因(yin)(yin)此(ci),具體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)情(qing)(qing)況(kuang)請參見(jian)(jian)說(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)與(yu)技(ji)術(shu)支持聯(lian)(lian)系(xi)(xi)確認。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)般(ban)(ban)要(yao)(yao)(yao)(yao)求(qiu):1)于(yu)(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)無(wu)(wu)(wu)(wu)溶(rong)血(xue)(xue)(xue)、脂(zhi)(zhi)血(xue)(xue)(xue)、的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現(xian)象。組(zu)(zu)織(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)將(jiang)血(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)洗(xi)凈(jing)擦(ca)干后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)稱重(zhong)(zhong)(zhong)(zhong)處理(li)(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)處理(li)(li)(li)(li)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)盡(jin)快進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且(qie)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)于(yu)(yu)(yu)(yu)(yu)(yu)冰盒(he)(he)(he)(he)(he)(he)(he)(he)之(zhi)(zhi)(zhi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang),待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。求(qiu)3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing),若(ruo)(ruo)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing),需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)離(li)(li)心(xin)(xin)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特(te)(te)殊(shu)指(zhi)(zhi)標(biao)(biao)(biao)除外(wai)(wai))。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)值(zhi)(zhi)(zhi)應(ying)(ying)(ying)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)線(xian)性(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)圍(wei)內,若(ruo)(ruo)超(chao)過(guo)(guo)線(xian)性(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)圍(wei),需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)稀(xi)釋(shi)(shi)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若(ruo)(ruo)低于(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)(fan)圍(wei),需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)增加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)濃(nong)(nong)度(du)(du)(du)(du)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)(fa)(fa)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)度(du)(du)(du)(du)法(fa)(fa)(fa)(fa)(fa)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie)(bie)(bie)?分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)度(du)(du)(du)(du)法(fa)(fa)(fa)(fa)(fa):主流(liu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)1mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)(yi)器(qi)(qi)(qi)(qi)(qi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)度(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji),能(neng)(neng)(neng)夠(gou)大(da)限度(du)(du)(du)(du)地滿(man)足絕大(da)多數(shu)(shu)(shu)用(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)。微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)(fa)(fa):主流(liu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)0.2mL,測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)儀(yi)(yi)器(qi)(qi)(qi)(qi)(qi)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)度(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji),測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)更(geng)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)簡便(bian)快捷。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)規(gui)格中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)100管(guan)(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)50管(guan)(guan)(guan)(guan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)什么意(yi)(yi)思?(1)50管(guan)(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):50管(guan)(guan)(guan)(guan)指(zhi)(zhi)能(neng)(neng)(neng)夠(gou)做(zuo)50次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)(zhi)可(ke)(ke)(ke)(ke)以(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)48個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)。如(ru)(ru)(ru)果(guo)每(mei)個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)重(zhong)(zhong)(zhong)(zhong)復(fu)(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci),那(nei)么只(zhi)能(neng)(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)16個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)。其(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)(you)(you)(you)2次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)空白管(guan)(guan)(guan)(guan)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)者對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)。值(zhi)(zhi)(zhi)得特(te)(te)別(bie)(bie)(bie)注(zhu)意(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)(shi),50管(guan)(guan)(guan)(guan)/24樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由于(yu)(yu)(yu)(yu)(yu)(yu)每(mei)個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)做(zuo)對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)(zhao),因(yin)(yin)此(ci)能(neng)(neng)(neng)夠(gou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)僅為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)8個(ge)(ge)(ge)(ge)(假(jia)設(she)做(zuo)3次(ci)(ci)重(zhong)(zhong)(zhong)(zhong)復(fu)(fu))(2)100管(guan)(guan)(guan)(guan)/96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang):100管(guan)(guan)(guan)(guan)指(zhi)(zhi)能(neng)(neng)(neng)夠(gou)做(zuo)100次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),96樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)指(zhi)(zhi)可(ke)(ke)(ke)(ke)以(yi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)96個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin),如(ru)(ru)(ru)果(guo)每(mei)個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)重(zhong)(zhong)(zhong)(zhong)復(fu)(fu)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)3次(ci)(ci),那(nei)么只(zhi)能(neng)(neng)(neng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)32個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)。其(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)有(you)(you)(you)(you)(you)(you)4次(ci)(ci)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)空白管(guan)(guan)(guan)(guan)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)者對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)。注(zhu)意(yi)(yi)由于(yu)(yu)(yu)(yu)(yu)(yu)瓶(ping)(ping)璧(bi)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)移(yi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)器(qi)(qi)(qi)(qi)(qi)吸(xi)(xi)附等(deng)(deng),通(tong)常(chang)(chang)(chang)(chang)(chang)(chang)要(yao)(yao)(yao)(yao)比(bi)(bi)(bi)(bi)(bi)理(li)(li)(li)(li)論(lun)(lun)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)次(ci)(ci)數(shu)(shu)(shu)少(shao)1~3次(ci)(ci)。其(qi)(qi)它(ta)規(gui)格的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)含(han)(han)義(yi)依次(ci)(ci)類(lei)(lei)(lei)推(tui)。值(zhi)(zhi)(zhi)得特(te)(te)別(bie)(bie)(bie)注(zhu)意(yi)(yi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)(shi),100管(guan)(guan)(guan)(guan)/48樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)由于(yu)(yu)(yu)(yu)(yu)(yu)每(mei)個(ge)(ge)(ge)(ge)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)都(dou)需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)做(zuo)對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)(zhao),因(yin)(yin)此(ci)能(neng)(neng)(neng)夠(gou)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)僅為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)16個(ge)(ge)(ge)(ge)(假(jia)設(she)做(zuo)3次(ci)(ci)重(zhong)(zhong)(zhong)(zhong)復(fu)(fu))。如(ru)(ru)(ru)何(he)(he)防(fang)(fang)止(zhi)(zhi)(zhi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)失(shi)(shi)(shi)效(xiao)?????不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)過(guo)(guo)早訂購(gou),以(yi)免試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)失(shi)(shi)(shi)效(xiao).收到試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou),檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)查(cha)外(wai)(wai)包裝(zhuang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)正(zheng)常(chang)(chang)(chang)(chang)(chang)(chang)。馬(ma)(ma)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)打開,按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)說(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)核對(dui)(dui)(dui)(dui)(dui)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)正(zheng)確,注(zhu)意(yi)(yi)瓶(ping)(ping)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)狀(zhuang)態(tai)(tai)(tai)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)與(yu)說(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)一(yi)(yi)(yi)(yi)致(zhi)。如(ru)(ru)(ru)果(guo)有(you)(you)(you)(you)(you)(you)問(wen)題,或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)者懷疑,馬(ma)(ma)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)聯(lian)(lian)系(xi)(xi)公司銷售,進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)確認。如(ru)(ru)(ru)果(guo)沒(mei)有(you)(you)(you)(you)(you)(you)問(wen)題,請務必(bi)按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)說(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)。注(zhu)意(yi)(yi),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji),可(ke)(ke)(ke)(ke)能(neng)(neng)(neng)需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)常(chang)(chang)(chang)(chang)(chang)(chang)溫(25°℃)、4℃和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)-20℃,務必(bi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)要(yao)(yao)(yao)(yao)求(qiu)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)要(yao)(yao)(yao)(yao)整個(ge)(ge)(ge)(ge)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)放置(zhi)(zhi)于(yu)(yu)(yu)(yu)(yu)(yu)冰箱(xiang)。如(ru)(ru)(ru)何(he)(he)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)預測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)?(1)務必(bi)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)7個(ge)(ge)(ge)(ge)工(gong)作(zuo)(zuo)日內進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)預測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),以(yi)確定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)該(gai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)適合(he)(he)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin),以(yi)免造(zao)成(cheng)(cheng)(cheng)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)浪(lang)費(fei)。(2)選(xuan)(xuan)擇(ze)2~3個(ge)(ge)(ge)(ge)預期差(cha)異比(bi)(bi)(bi)(bi)(bi)較(jiao)大(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin),嚴格按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)說(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)操作(zuo)(zuo),注(zhu)意(yi)(yi)儀(yi)(yi)器(qi)(qi)(qi)(qi)(qi)工(gong)作(zuo)(zuo)狀(zhuang)態(tai)(tai)(tai),操作(zuo)(zuo)規(gui)范(fan)(fan),控(kong)制(zhi)(zhi)(zhi)(zhi)好(hao)(hao)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)條件(jian),尤其(qi)(qi)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)溫度(du)(du)(du)(du)。(3)如(ru)(ru)(ru)實(shi)(shi)與(yu)公司銷售溝通(tong)預測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結果(guo),公司技(ji)術(shu)人(ren)員會確認測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結果(guo)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)正(zheng)常(chang)(chang)(chang)(chang)(chang)(chang),是(shi)(shi)(shi)(shi)(shi)(shi)(shi)否(fou)需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)調(diao)整,如(ru)(ru)(ru)何(he)(he)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)調(diao)整,從而(er)確保(bao)(bao)(bao)(bao)(bao)您的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結果(guo)可(ke)(ke)(ke)(ke)靠(kao)。熒(ying)(ying)(ying)(ying)(ying)(ying)(ying) 光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang) 分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen) 析(xi)(xi)(xi)(xi)(xi)(xi)(xi) 法(fa)(fa)(fa)(fa)(fa)?????????熒(ying)(ying)(ying)(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)法(fa)(fa)(fa)(fa)(fa)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)指(zhi)(zhi)利用(yong)(yong)(yong)(yong)(yong)(yong)某(mou)(mou)些(xie)(xie)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)被(bei)(bei)紫外(wai)(wai)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)照(zhao)(zhao)(zhao)(zhao)(zhao)射(she)(she)(she)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)處于(yu)(yu)(yu)(yu)(yu)(yu)激發(fa)(fa)態(tai)(tai)(tai)，激發(fa)(fa)態(tai)(tai)(tai)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)經(jing)歷一(yi)(yi)(yi)(yi)個(ge)(ge)(ge)(ge)碰撞及(ji)發(fa)(fa)射(she)(she)(she)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)去激發(fa)(fa)過(guo)(guo)程所(suo)發(fa)(fa)生(sheng)(sheng)(sheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)能(neng)(neng)(neng)反(fan)(fan)(fan)映出(chu)該(gai)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)特(te)(te)性(xing)(xing)(xing)(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)熒(ying)(ying)(ying)(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)，可(ke)(ke)(ke)(ke)以(yi)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)方(fang)(fang)法(fa)(fa)(fa)(fa)(fa)。由于(yu)(yu)(yu)(yu)(yu)(yu)有(you)(you)(you)(you)(you)(you)些(xie)(xie)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)本(ben)(ben)(ben)(ben)(ben)(ben)身不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)發(fa)(fa)射(she)(she)(she)熒(ying)(ying)(ying)(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)（或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)熒(ying)(ying)(ying)(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)很(hen)弱），這(zhe)就(jiu)需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)把不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)發(fa)(fa)射(she)(she)(she)熒(ying)(ying)(ying)(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)轉化(hua)(hua)(hua)(hua)(hua)成(cheng)(cheng)(cheng)能(neng)(neng)(neng)發(fa)(fa)射(she)(she)(she)熒(ying)(ying)(ying)(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。例(li)(li)(li)如(ru)(ru)(ru)用(yong)(yong)(yong)(yong)(yong)(yong)某(mou)(mou)些(xie)(xie)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)（如(ru)(ru)(ru)熒(ying)(ying)(ying)(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)染料），使(shi)(shi)(shi)其(qi)(qi)與(yu)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)發(fa)(fa)射(she)(she)(she)熒(ying)(ying)(ying)(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)生(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng)絡(luo)(luo)合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)，各種(zhong)絡(luo)(luo)合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)能(neng)(neng)(neng)發(fa)(fa)射(she)(she)(she)熒(ying)(ying)(ying)(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)，再(zai)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)。因(yin)(yin)此(ci)熒(ying)(ying)(ying)(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)，對(dui)(dui)(dui)(dui)(dui)一(yi)(yi)(yi)(yi)些(xie)(xie)原(yuan)來(lai)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)發(fa)(fa)熒(ying)(ying)(ying)(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)無(wu)(wu)(wu)(wu)機物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)有(you)(you)(you)(you)(you)(you)機物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)熒(ying)(ying)(ying)(ying)(ying)(ying)(ying)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)打開了(le)(le)大(da)門，擴展了(le)(le)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)范(fan)(fan)圍(wei)。比(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min)有(you)(you)(you)(you)(you)(you)哪些(xie)(xie)規(gui)格?(1)常(chang)(chang)(chang)(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)比(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min),如(ru)(ru)(ru)1mL和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)3mL等(deng)(deng),常(chang)(chang)(chang)(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)(fa)(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)都(dou)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)選(xuan)(xuan)用(yong)(yong)(yong)(yong)(yong)(yong)1mL體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi),只(zhi)能(neng)(neng)(neng)用(yong)(yong)(yong)(yong)(yong)(yong)1mL比(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)可(ke)(ke)(ke)(ke)以(yi)用(yong)(yong)(yong)(yong)(yong)(yong)更(geng)大(da)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min),否(fou)則試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)量(liang)(liang)(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)夠(gou),無(wu)(wu)(wu)(wu)法(fa)(fa)(fa)(fa)(fa)正(zheng)常(chang)(chang)(chang)(chang)(chang)(chang)比(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)(se)。(2)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)比(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min),如(ru)(ru)(ru)0.25mL和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)0.5mL等(deng)(deng),微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)法(fa)(fa)(fa)(fa)(fa)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)都(dou)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)選(xuan)(xuan)用(yong)(yong)(yong)(yong)(yong)(yong)0.25mL比(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min)。一(yi)(yi)(yi)(yi)般(ban)(ban)客(ke)戶(hu)都(dou)選(xuan)(xuan)擇(ze)使(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)標(biao)(biao)(biao)儀(yi)(yi)(需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)自(zi)(zi)備(bei)酶(mei)標(biao)(biao)(biao)板(ban))(3)紫外(wai)(wai)波長(chang)(chang)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)石英比(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)可(ke)(ke)(ke)(ke)以(yi)用(yong)(yong)(yong)(yong)(yong)(yong)普通(tong)玻璃比(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min)。(4)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)規(gui)格的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min),其(qi)(qi)外(wai)(wai)觀大(da)小(xiao)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)一(yi)(yi)(yi)(yi)致(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de),都(dou)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)1cm光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)徑(也有(you)(you)(you)(you)(you)(you)0.5cm光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)徑的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)常(chang)(chang)(chang)(chang)(chang)(chang)見(jian)(jian),也可(ke)(ke)(ke)(ke)以(yi)用(yong)(yong)(yong)(yong)(yong)(yong),但(dan)(dan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)時要(yao)(yao)(yao)(yao)注(zhu)意(yi)(yi)修改(gai)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)公式),其(qi)(qi)內容積(ji)(ji)差(cha)異在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)于(yu)(yu)(yu)(yu)(yu)(yu)壁的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)厚(hou)度(du)(du)(du)(du)。代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)優(you)點(1)更(geng)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)省事兒,完(wan)全(quan)(quan)(quan)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)必(bi)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)犯(fan)難。用(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)只(zhi)要(yao)(yao)(yao)(yao)提(ti)(ti)(ti)(ti)(ti)(ti)供樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)想測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)指(zhi)(zhi)標(biao)(biao)(biao),就(jiu)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)再(zai)需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)操心(xin)(xin)了(le)(le),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)再(zai)受(shou)(shou)到操作(zuo)(zuo)技(ji)能(neng)(neng)(neng)、儀(yi)(yi)器(qi)(qi)(qi)(qi)(qi)設(she)備(bei)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)時間的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限制(zhi)(zhi)(zhi)(zhi),就(jiu)等(deng)(deng)著拿到數(shu)(shu)(shu)據寫論(lun)(lun)文。(2)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)數(shu)(shu)(shu)據更(geng)有(you)(you)(you)(you)(you)(you)保(bao)(bao)(bao)(bao)(bao)障。嚴格的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)管(guan)(guan)(guan)(guan)理(li)(li)(li)(li)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi),從而(er)保(bao)(bao)(bao)(bao)(bao)證(zheng)了(le)(le)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)結果(guo)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)真實(shi)(shi)可(ke)(ke)(ke)(ke)靠(kao)。我們還建(jian)立了(le)(le)免費(fei)預測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)制(zhi)(zhi)(zhi)(zhi)度(du)(du)(du)(du)。(3)防(fang)(fang)止(zhi)(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)損(sun)失(shi)(shi)(shi)無(wu)(wu)(wu)(wu)論(lun)(lun)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)用(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)自(zi)(zi)己完(wan)成(cheng)(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)全(quan)(quan)(quan)過(guo)(guo)程,還是(shi)(shi)(shi)(shi)(shi)(shi)(shi)購(gou)買(mai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)完(wan)成(cheng)(cheng)(cheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding),都(dou)可(ke)(ke)(ke)(ke)能(neng)(neng)(neng)因(yin)(yin)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)缺乏(fa)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)經(jing)驗,導(dao)(dao)(dao)致(zhi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)失(shi)(shi)(shi)敗,從而(er)損(sun)失(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)情(qing)(qing)況(kuang)。對(dui)(dui)(dui)(dui)(dui)于(yu)(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)命科學(xue)(xue)(xue)(xue)研(yan)(yan)究而(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)＊珍貴的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)。樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)一(yi)(yi)(yi)(yi)旦損(sun)失(shi)(shi)(shi),輕則造(zao)成(cheng)(cheng)(cheng)研(yan)(yan)究進(jin)(jin)(jin)(jin)(jin)度(du)(du)(du)(du)被(bei)(bei)耽擱,重(zhong)(zhong)(zhong)(zhong)則造(zao)成(cheng)(cheng)(cheng)研(yan)(yan)究完(wan)全(quan)(quan)(quan)失(shi)(shi)(shi)敗!代(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)能(neng)(neng)(neng)夠(gou)充分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)保(bao)(bao)(bao)(bao)(bao)證(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)(cheng)功(gong)(gong),從而(er)防(fang)(fang)止(zhi)(zhi)(zhi)了(le)(le)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)損(sun)失(shi)(shi)(shi)。當(dang)然(ran),樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)成(cheng)(cheng)(cheng)功(gong)(gong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)前提(ti)(ti)(ti)(ti)(ti)(ti),而(er)且(qie)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)只(zhi)有(you)(you)(you)(you)(you)(you)用(yong)(yong)(yong)(yong)(yong)(yong)戶(hu)自(zi)(zi)己才能(neng)(neng)(neng)控(kong)制(zhi)(zhi)(zhi)(zhi)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)保(bao)(bao)(bao)(bao)(bao)證(zheng)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)本(ben)(ben)(ben)(ben)(ben)(ben)原(yuan)理(li)(li)(li)(li)??生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he),又稱生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he),是(shi)(shi)(shi)(shi)(shi)(shi)(shi)利用(yong)(yong)(yong)(yong)(yong)(yong)特(te)(te)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)成(cheng)(cheng)(cheng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)、動力學(xue)(xue)(xue)(xue)及(ji)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)反(fan)(fan)(fan)應(ying)(ying)(ying)等(deng)(deng)技(ji)術(shu)制(zhi)(zhi)(zhi)(zhi)備(bei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he),用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)生(sheng)(sheng)(sheng)理(li)(li)(li)(li)學(xue)(xue)(xue)(xue)、病理(li)(li)(li)(li)學(xue)(xue)(xue)(xue)、藥理(li)(li)(li)(li)學(xue)(xue)(xue)(xue)等(deng)(deng)領域(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)特(te)(te)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)包括底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、酶(mei)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)、探針等(deng)(deng)多個(ge)(ge)(ge)(ge)組(zu)(zu)成(cheng)(cheng)(cheng)部(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen),其(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)＊常(chang)(chang)(chang)(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)法(fa)(fa)(fa)(fa)(fa)。底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)法(fa)(fa)(fa)(fa)(fa)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)基(ji)本(ben)(ben)(ben)(ben)(ben)(ben)原(yuan)理(li)(li)(li)(li)。在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)該(gai)過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)與(yu)酶(mei)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)細(xi)(xi)(xi)(xi)(xi)(xi)胞膜上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)結合(he)(he)形(xing)成(cheng)(cheng)(cheng)基(ji)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)酶(mei),進(jin)(jin)(jin)(jin)(jin)而(er)形(xing)成(cheng)(cheng)(cheng)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)復(fu)(fu)合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)。隨著時間的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)推(tui)移(yi),底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)-酶(mei)復(fu)(fu)合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)逐(zhu)漸(jian)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)解(jie)(jie)(jie)(jie),釋(shi)(shi)放出(chu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)小(xiao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)子(zi)大(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)未完(wan)全(quan)(quan)(quan)消(xiao)化(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)。未完(wan)全(quan)(quan)(quan)消(xiao)化(hua)(hua)(hua)(hua)(hua)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)可(ke)(ke)(ke)(ke)以(yi)被(bei)(bei)一(yi)(yi)(yi)(yi)系(xi)(xi)列的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)酶(mei)代(dai)謝,同(tong)(tong)(tong)時底(di)(di)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、酶(mei)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)代(dai)謝產物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)都(dou)會對(dui)(dui)(dui)(dui)(dui)復(fu)(fu)合(he)(he)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)穩定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)產生(sheng)(sheng)(sheng)影響,從而(er)影響檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)結果(guo)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)可(ke)(ke)(ke)(ke)以(yi)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing)有(you)(you)(you)(you)(you)(you)哪些(xie)(xie)?? ?生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)實(shi)(shi)驗原(yuan)理(li)(li)(li)(li)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)基(ji)于(yu)(yu)(yu)(yu)(yu)(yu)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)反(fan)(fan)(fan)應(ying)(ying)(ying),理(li)(li)(li)(li)論(lun)(lun)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)受(shou)(shou)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)種(zhong)屬(shu)(shu)及(ji)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)限制(zhi)(zhi)(zhi)(zhi)。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)通(tong)常(chang)(chang)(chang)(chang)(chang)(chang)只(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)類(lei)(lei)(lei)型(xing)(xing)(xing),不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)種(zhong)屬(shu)(shu)。但(dan)(dan)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)某(mou)(mou)些(xie)(xie)指(zhi)(zhi)標(biao)(biao)(biao)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)種(zhong)屬(shu)(shu)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)類(lei)(lei)(lei)型(xing)(xing)(xing)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)方(fang)(fang)式不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)。因(yin)(yin)此(ci),具體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)情(qing)(qing)況(kuang)請參見(jian)(jian)說(shuo)明(ming)(ming)(ming)書(shu)(shu)(shu)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)與(yu)技(ji)術(shu)支持聯(lian)(lian)系(xi)(xi)確認。生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)一(yi)(yi)(yi)(yi)般(ban)(ban)要(yao)(yao)(yao)(yao)求(qiu):1)于(yu)(yu)(yu)(yu)(yu)(yu)生(sheng)(sheng)(sheng)化(hua)(hua)(hua)(hua)(hua)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)而(er)言,樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)＊-好(hao)(hao)無(wu)(wu)(wu)(wu)溶(rong)血(xue)(xue)(xue)、脂(zhi)(zhi)血(xue)(xue)(xue)、黃-疸(dan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)現(xian)象。組(zu)(zu)織(zhi)(zhi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)將(jiang)血(xue)(xue)(xue)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)洗(xi)凈(jing)擦(ca)干后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)稱重(zhong)(zhong)(zhong)(zhong)處理(li)(li)(li)(li)。2)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)處理(li)(li)(li)(li)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)＊-好(hao)(hao)盡(jin)快進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),且(qie)保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)于(yu)(yu)(yu)(yu)(yu)(yu)冰盒(he)(he)(he)(he)(he)(he)(he)(he)之(zhi)(zhi)(zhi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang),待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing),若(ruo)(ruo)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)澄(cheng)清(qing)(qing)(qing)(qing)(qing)(qing),需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)離(li)(li)心(xin)(xin)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(特(te)(te)殊(shu)指(zhi)(zhi)標(biao)(biao)(biao)除外(wai)(wai))。4)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)值(zhi)(zhi)(zhi)應(ying)(ying)(ying)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)線(xian)性(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)圍(wei)內,若(ruo)(ruo)超(chao)過(guo)(guo)線(xian)性(xing)(xing)(xing)(xing)(xing)(xing)范(fan)(fan)圍(wei),需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)稀(xi)釋(shi)(shi)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce),若(ruo)(ruo)低于(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)范(fan)(fan)圍(wei),需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)增加(jia)(jia)(jia)(jia)(jia)(jia)(jia)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)濃(nong)(nong)度(du)(du)(du)(du)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。微(wei)(wei)(wei)(wei)(wei)(wei) 量(liang)(liang)(liang)(liang)(liang)(liang) 分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen) 析(xi)(xi)(xi)(xi)(xi)(xi)(xi) 法(fa)(fa)(fa)(fa)(fa)? ?????????微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)(Microanalysis)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)，其(qi)(qi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)重(zhong)(zhong)(zhong)(zhong)量(liang)(liang)(liang)(liang)(liang)(liang)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)1-10mg的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)方(fang)(fang)法(fa)(fa)(fa)(fa)(fa)稱為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)。有(you)(you)(you)(you)(you)(you)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)之(zhi)(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)，定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)性(xing)(xing)(xing)(xing)(xing)(xing)常(chang)(chang)(chang)(chang)(chang)(chang)采用(yong)(yong)(yong)(yong)(yong)(yong)點滴反(fan)(fan)(fan)應(ying)(ying)(ying)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)顯微(wei)(wei)(wei)(wei)(wei)(wei)結晶反(fan)(fan)(fan)應(ying)(ying)(ying)等(deng)(deng)靈敏度(du)(du)(du)(du)較(jiao)高(gao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)方(fang)(fang)法(fa)(fa)(fa)(fa)(fa)，定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)常(chang)(chang)(chang)(chang)(chang)(chang)用(yong)(yong)(yong)(yong)(yong)(yong)重(zhong)(zhong)(zhong)(zhong)量(liang)(liang)(liang)(liang)(liang)(liang)、容量(liang)(liang)(liang)(liang)(liang)(liang)及(ji)儀(yi)(yi)器(qi)(qi)(qi)(qi)(qi)等(deng)(deng)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)方(fang)(fang)法(fa)(fa)(fa)(fa)(fa)。在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)以(yi)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)重(zhong)(zhong)(zhong)(zhong)量(liang)(liang)(liang)(liang)(liang)(liang)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)類(lei)(lei)(lei)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)還有(you)(you)(you)(you)(you)(you)半微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)超(chao)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)等(deng)(deng)。微(wei)(wei)(wei)(wei)(wei)(wei) 量(liang)(liang)(liang)(liang)(liang)(liang) 和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he) 超(chao) 微(wei)(wei)(wei)(wei)(wei)(wei) 量(liang)(liang)(liang)(liang)(liang)(liang) 分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen) 析(xi)(xi)(xi)(xi)(xi)(xi)(xi)????????微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)、超(chao)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)常(chang)(chang)(chang)(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)主要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)差(cha)別(bie)(bie)(bie)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)。一(yi)(yi)(yi)(yi)般(ban)(ban)稱樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)0.01克(ke)~0.001克(ke)者為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)，小(xiao)于(yu)(yu)(yu)(yu)(yu)(yu)0.001克(ke)者為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)超(chao)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)。正(zheng)因(yin)(yin)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)很(hen)少(shao)，所(suo)以(yi)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)操作(zuo)(zuo)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)也和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)常(chang)(chang)(chang)(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)很(hen)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)相同(tong)(tong)(tong)。例(li)(li)(li)如(ru)(ru)(ru)沉(chen)淀(dian)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)溶(rong)解(jie)(jie)(jie)(jie)度(du)(du)(du)(du)、指(zhi)(zhi)示(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)誤(wu)差(cha)等(deng)(deng)對(dui)(dui)(dui)(dui)(dui)常(chang)(chang)(chang)(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)影響可(ke)(ke)(ke)(ke)以(yi)忽略，而(er)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)超(chao)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)時就(jiu)會引起很(hen)大(da)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)誤(wu)差(cha)。尤其(qi)(qi)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)超(chao)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)要(yao)(yao)(yao)(yao)求(qiu)更(geng)嚴格，須(xu)(xu)考(kao)慮到溶(rong)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)本(ben)(ben)(ben)(ben)(ben)(ben)身在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)空氣中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)蒸(zheng)發(fa)(fa)，因(yin)(yin)此(ci)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)滴定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)時必(bi)緩在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)潮濕的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)空氣中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)。????????一(yi)(yi)(yi)(yi)般(ban)(ban)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)用(yong)(yong)(yong)(yong)(yong)(yong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)器(qi)(qi)(qi)(qi)(qi)皿(min)(min)(min)都(dou)很(hen)小(xiao)，按(an)(an)(an)(an)(an)(an)(an)其(qi)(qi)要(yao)(yao)(yao)(yao)求(qiu)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)有(you)(you)(you)(you)(you)(you)各種(zhong)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)同(tong)(tong)(tong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)形(xing)狀(zhuang)。各種(zhong)儀(yi)(yi)器(qi)(qi)(qi)(qi)(qi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)法(fa)(fa)(fa)(fa)(fa)經(jing)過(guo)(guo)適當(dang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)改(gai)進(jin)(jin)(jin)(jin)(jin)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)，可(ke)(ke)(ke)(ke)以(yi)直接(jie)應(ying)(ying)(ying)用(yong)(yong)(yong)(yong)(yong)(yong)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)。超(chao)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)由于(yu)(yu)(yu)(yu)(yu)(yu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)太少(shao)，因(yin)(yin)此(ci)必(bi)須(xu)(xu)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)顯微(wei)(wei)(wei)(wei)(wei)(wei)鏡(jing)下(xia)(xia)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)所(suo)有(you)(you)(you)(you)(you)(you)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)操作(zuo)(zuo)(如(ru)(ru)(ru)沉(chen)淀(dian)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)濡定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)等(deng)(deng))，某(mou)(mou)些(xie)(xie)儀(yi)(yi)器(qi)(qi)(qi)(qi)(qi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)(如(ru)(ru)(ru)電(dian)(dian)位滴定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)，電(dian)(dian)解(jie)(jie)(jie)(jie)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)電(dian)(dian)導(dao)(dao)(dao)等(deng)(deng))，也可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)來(lai)作(zuo)(zuo)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)超(chao)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)之(zhi)(zhi)(zhi)用(yong)(yong)(yong)(yong)(yong)(yong)。????????微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)研(yan)(yan)究工(gong)作(zuo)(zuo)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)用(yong)(yong)(yong)(yong)(yong)(yong)得極廣。尤其(qi)(qi)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)稀(xi)有(you)(you)(you)(you)(you)(you)元素(su)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)研(yan)(yan)究時，在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)解(jie)(jie)(jie)(jie)決地球(qiu)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)同(tong)(tong)(tong)題，研(yan)(yan)究某(mou)(mou)些(xie)(xie)稀(xi)有(you)(you)(you)(you)(you)(you)元素(su)礦物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)粗成(cheng)(cheng)(cheng)及(ji)其(qi)(qi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)配(pei)規(gui)律時，往往只(zhi)能(neng)(neng)(neng)得到極少(shao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)，在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)這(zhe)選(xuan)(xuan)種(zhong)情(qing)(qing)況(kuang)下(xia)(xia)一(yi)(yi)(yi)(yi)般(ban)(ban)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)常(chang)(chang)(chang)(chang)(chang)(chang)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)法(fa)(fa)(fa)(fa)(fa)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)無(wu)(wu)(wu)(wu)法(fa)(fa)(fa)(fa)(fa)進(jin)(jin)(jin)(jin)(jin)行(xing)(xing)(xing)(xing)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)，只(zhi)有(you)(you)(you)(you)(you)(you)靠(kao)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)法(fa)(fa)(fa)(fa)(fa)來(lai)解(jie)(jie)(jie)(jie)決。另外(wai)(wai)，在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)化(hua)(hua)(hua)(hua)(hua)學(xue)(xue)(xue)(xue)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)析(xi)(xi)(xi)(xi)(xi)(xi)(xi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)應(ying)(ying)(ying)用(yong)(yong)(yong)(yong)(yong)(yong)更(geng)是(shi)(shi)(shi)(shi)(shi)(shi)(shi)普遍ATP含(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)盒(he)(he)(he)(he)(he)(he)(he)(he)本(ben)(ben)(ben)(ben)(ben)(ben)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)科研(yan)(yan)實(shi)(shi)驗,不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)臨(lin)床。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)意(yi)(yi)義(yi):輔酶(mei)NAD(H)廣泛存(cun)(cun)(cun)(cun)(cun)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)于(yu)(yu)(yu)(yu)(yu)(yu)動物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、植(zhi)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)、微(wei)(wei)(wei)(wei)(wei)(wei)生(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)培養細(xi)(xi)(xi)(xi)(xi)(xi)胞中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),NAD+是(shi)(shi)(shi)(shi)(shi)(shi)(shi)糖酵解(jie)(jie)(jie)(jie)(EMP)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)三(san)(san)(san)(san)羧酸(suan)(suan)循(xun)環(huan)(TCA)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)主要(yao)(yao)(yao)(yao)氫受(shou)(shou)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti),生(sheng)(sheng)(sheng)成(cheng)(cheng)(cheng)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?NADH?經(jing)呼(hu)吸(xi)(xi)電(dian)(dian)子(zi)鏈(lian)(ETC)傳遞(di)把電(dian)(dian)子(zi)交給氧,在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)合(he)(he)成(cheng)(cheng)(cheng)?ATP?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)同(tong)(tong)(tong)時,形(xing)成(cheng)(cheng)(cheng)大(da)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)?ROS,同(tong)(tong)(tong)時?NADH?再(zai)生(sheng)(sheng)(sheng)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?NAD+。糖、脂(zhi)(zhi)、蛋白質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)三(san)(san)(san)(san)大(da)代(dai)謝物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)解(jie)(jie)(jie)(jie)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)氧化(hua)(hua)(hua)(hua)(hua)反(fan)(fan)(fan)應(ying)(ying)(ying)絕大(da)部(bu)(bu)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)通(tong)過(guo)(guo)這(zhe)一(yi)(yi)(yi)(yi)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)完(wan)成(cheng)(cheng)(cheng)。NAD(H)含(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH/NAD+比(bi)(bi)(bi)(bi)(bi)值(zhi)(zhi)(zhi)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)高(gao)低可(ke)(ke)(ke)(ke)用(yong)(yong)(yong)(yong)(yong)(yong)于(yu)(yu)(yu)(yu)(yu)(yu)評價糖酵解(jie)(jie)(jie)(jie)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?TCA?循(xun)環(huan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)強弱。較(jiao)高(gao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)NAD(H)及(ji)?NADH/NAD+比(bi)(bi)(bi)(bi)(bi)值(zhi)(zhi)(zhi)說(shuo)明(ming)(ming)(ming)細(xi)(xi)(xi)(xi)(xi)(xi)胞呼(hu)吸(xi)(xi)耗氧量(liang)(liang)(liang)(liang)(liang)(liang)較(jiao)高(gao),處于(yu)(yu)(yu)(yu)(yu)(yu)過(guo)(guo)氧化(hua)(hua)(hua)(hua)(hua)狀(zhuang)態(tai)(tai)(tai)。此(ci)外(wai)(wai),NADH/NAD+比(bi)(bi)(bi)(bi)(bi)值(zhi)(zhi)(zhi)升(sheng)高(gao)也可(ke)(ke)(ke)(ke)抑(yi)制(zhi)(zhi)(zhi)(zhi)糖酵解(jie)(jie)(jie)(jie)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?TCA?循(xun)環(huan)。另外(wai)(wai),NAD+降解(jie)(jie)(jie)(jie)產物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)對(dui)(dui)(dui)(dui)(dui)細(xi)(xi)(xi)(xi)(xi)(xi)胞信號傳導(dao)(dao)(dao)、代(dai)謝和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)基(ji)因(yin)(yin)表達(da)等(deng)(deng)具有(you)(you)(you)(you)(you)(you)重(zhong)(zhong)(zhong)(zhong)要(yao)(yao)(yao)(yao)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)調(diao)控(kong)作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong)。測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)原(yuan)理(li)(li)(li)(li):分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)別(bie)(bie)(bie)用(yong)(yong)(yong)(yong)(yong)(yong)酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)品(pin)(pin)(pin)(pin)(pin)(pin)(pin)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH,NADH?通(tong)過(guo)(guo)?PMS?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)遞(di)氫作(zuo)(zuo)用(yong)(yong)(yong)(yong)(yong)(yong),還原(yuan)氧化(hua)(hua)(hua)(hua)(hua)型(xing)(xing)(xing)噻唑藍(MTT)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)甲瓚,在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)?570nm?下(xia)(xia)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)吸(xi)(xi)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)值(zhi)(zhi)(zhi);而(er)?NAD+可(ke)(ke)(ke)(ke)被(bei)(bei)乙醇脫氫酶(mei)還原(yuan)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?NADH,進(jin)(jin)(jin)(jin)(jin)一(yi)(yi)(yi)(yi)步采用(yong)(yong)(yong)(yong)(yong)(yong)?MTT?還原(yuan)法(fa)(fa)(fa)(fa)(fa)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)自(zi)(zi)備(bei)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)儀(yi)(yi)器(qi)(qi)(qi)(qi)(qi)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)用(yong)(yong)(yong)(yong)(yong)(yong)品(pin)(pin)(pin)(pin)(pin)(pin)(pin):酶(mei)標(biao)(biao)(biao)儀(yi)(yi)、臺式離(li)(li)心(xin)(xin)機、移(yi)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)器(qi)(qi)(qi)(qi)(qi)、96?孔板(ban)、研(yan)(yan)缽、冰和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)蒸(zheng)餾水(shui)(shui)。試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)組(zu)(zu)成(cheng)(cheng)(cheng)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)配(pei)制(zhi)(zhi)(zhi)(zhi):酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping),4℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun);堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping),4℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)?10 mL×1?瓶(ping)(ping),4℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)二(er):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)?3 mL×1?瓶(ping)(ping),4℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)三(san)(san)(san)(san):粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping)(ping),-20?℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun),用(yong)(yong)(yong)(yong)(yong)(yong)時加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?3mL?蒸(zheng)餾水(shui)(shui),混(hun)勻,用(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)一(yi)(yi)(yi)(yi)周;試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)四(si):粉劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)×1?瓶(ping)(ping),4?℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun),用(yong)(yong)(yong)(yong)(yong)(yong)時加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?3mL?蒸(zheng)餾水(shui)(shui),混(hun)勻,用(yong)(yong)(yong)(yong)(yong)(yong)不(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)(bu)完(wan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)?4℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)一(yi)(yi)(yi)(yi)周;試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)五(wu)(wu):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)?3.6mL×1?瓶(ping)(ping),4?℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu):液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)?30mL×1?瓶(ping)(ping),4?℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun);試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)七:液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)?50mL×1?瓶(ping)(ping),4?℃保(bao)(bao)(bao)(bao)(bao)存(cun)(cun)(cun)(cun)(cun)。NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):1?血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(mL):酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)例(li)(li)(li)(建(jian)議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)(yue)?0.1mL?血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)(jiang)),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?1mL?酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)浴(yu)(yu)(yu)?5min(蓋緊(jin)(jin),以(yi)防(fang)(fang)止(zhi)(zhi)(zhi)水(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi));冰浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)心(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?500μL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)(shi)之(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻,10000g 4?℃離(li)(li)心(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(mL):堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)例(li)(li)(li)(建(jian)議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)(yue)?0.1mL血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)(jiang)),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?1mL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)),95℃水(shui)(shui)浴(yu)(yu)(yu)?5min(蓋緊(jin)(jin),以(yi)防(fang)(fang)止(zhi)(zhi)(zhi)水(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi));冰浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)心(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?500μL?酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)(shi)之(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻,10000g 4?℃離(li)(li)心(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。2?組(zu)(zu)織(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)組(zu)(zu)織(zhi)(zhi)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(g):酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)例(li)(li)(li)(建(jian)議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)(yue)?0.1g?組(zu)(zu)織(zhi)(zhi),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?1mL酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)),冰浴(yu)(yu)(yu)研(yan)(yan)磨,95℃水(shui)(shui)浴(yu)(yu)(yu)?5min(蓋緊(jin)(jin),以(yi)防(fang)(fang)止(zhi)(zhi)(zhi)水(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi));冰浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)心(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?500μL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)(shi)之(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻,10000g 4?℃離(li)(li)心(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)組(zu)(zu)織(zhi)(zhi)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang)(g):堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?1:5~10?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)例(li)(li)(li)(建(jian)議(yi)(yi)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)約(yue)(yue)?0.1g?組(zu)(zu)織(zhi)(zhi),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?1mL堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)),冰浴(yu)(yu)(yu)研(yan)(yan)磨,95℃水(shui)(shui)浴(yu)(yu)(yu)?5min(蓋緊(jin)(jin),以(yi)防(fang)(fang)止(zhi)(zhi)(zhi)水(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi));冰浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)心(xin)(xin)10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?500μL?酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)(shi)之(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻,10000g 4?℃離(li)(li)心(xin)(xin)?10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。3?細(xi)(xi)(xi)(xi)(xi)(xi)胞或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):NAD+的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先收集細(xi)(xi)(xi)(xi)(xi)(xi)胞或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)到離(li)(li)心(xin)(xin)管(guan)(guan)(guan)(guan)內,棄(qi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)胞數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)(ge)):酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)例(li)(li)(li)(建(jian)議(yi)(yi)?500?萬細(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)胞加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?1mL?酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)),超(chao)聲波破碎(冰浴(yu)(yu)(yu),功(gong)(gong)率20%或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)?200W,超(chao)聲?3s,間隔?10s,重(zhong)(zhong)(zhong)(zhong)復(fu)(fu)?30?次(ci)(ci)),95℃水(shui)(shui)浴(yu)(yu)(yu)?5min(蓋緊(jin)(jin),以(yi)防(fang)(fang)止(zhi)(zhi)(zhi)水(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi));冰浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)心(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?500μL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)(shi)之(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻,10000g 4?℃離(li)(li)心(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。NADH?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu):先收集細(xi)(xi)(xi)(xi)(xi)(xi)胞或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)到離(li)(li)心(xin)(xin)管(guan)(guan)(guan)(guan)內,棄(qi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),按(an)(an)(an)(an)(an)(an)(an)照(zhao)(zhao)(zhao)(zhao)(zhao)細(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)胞數(shu)(shu)(shu)量(liang)(liang)(liang)(liang)(liang)(liang)(104?個(ge)(ge)(ge)(ge)):堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji)(mL)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?500~1000:1?的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)比(bi)(bi)(bi)(bi)(bi)例(li)(li)(li)(建(jian)議(yi)(yi)?500?萬細(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)胞加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?1mL?堿(jian)(jian)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)),超(chao)聲波破碎(冰浴(yu)(yu)(yu),功(gong)(gong)率20%或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)?200W,超(chao)聲?3s,間隔?10s,重(zhong)(zhong)(zhong)(zhong)復(fu)(fu)?30?次(ci)(ci)),95℃水(shui)(shui)浴(yu)(yu)(yu)?5min(蓋緊(jin)(jin),以(yi)防(fang)(fang)止(zhi)(zhi)(zhi)水(shui)(shui)分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)散(san)失(shi)(shi)(shi));冰浴(yu)(yu)(yu)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)冷卻(que)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou),10000g 4?℃離(li)(li)心(xin)(xin)?10min;取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?500μL?上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing)液(ye)(ye)(ye)(ye)(ye)(ye)(ye),加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?500μL?酸(suan)(suan)性(xing)(xing)(xing)(xing)(xing)(xing)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)使(shi)(shi)(shi)之(zhi)(zhi)(zhi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he),混(hun)勻,10000g 4?℃離(li)(li)心(xin)(xin)10min,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),置(zhi)(zhi)冰上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)待(dai)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)。充分(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)(fen)混(hun)勻,靜(jing)置(zhi)(zhi)?5min?后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou),20000g,25℃離(li)(li)心(xin)(xin)?5min,棄(qi)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)清(qing)(qing)(qing)(qing)(qing)(qing),沉(chen)淀(dian)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru):混(hun)勻,取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?200μL?轉移(yi)至微(wei)(wei)(wei)(wei)(wei)(wei)量(liang)(liang)(liang)(liang)(liang)(liang)石英比(bi)(bi)(bi)(bi)(bi)色(se)(se)(se)(se)(se)皿(min)(min)(min)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)?96?孔板(ban)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong),570nm?下(xia)(xia)讀(du)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)吸(xi)(xi)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)值(zhi)(zhi)(zhi)?A1?和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)吸(xi)(xi)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)值(zhi)(zhi)(zhi)?A2,計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)△A=A2-A1。注(zhu)意(yi)(yi)事項:1、如(ru)(ru)(ru)果(guo)一(yi)(yi)(yi)(yi)次(ci)(ci)性(xing)(xing)(xing)(xing)(xing)(xing)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)數(shu)(shu)(shu)較(jiao)多,可(ke)(ke)(ke)(ke)將(jiang)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)、二(er)、三(san)(san)(san)(san)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)四(si)按(an)(an)(an)(an)(an)(an)(an)比(bi)(bi)(bi)(bi)(bi)例(li)(li)(li)配(pei)成(cheng)(cheng)(cheng)混(hun)合(he)(he)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)。2、對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)步驟(zou)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)區別(bie)(bie)(bie):對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)完(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)、二(er)、三(san)(san)(san)(san)、四(si)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)五(wu)(wu)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)必(bi)須(xu)(xu)馬(ma)(ma)上(shang)(shang)(shang)(shang)(shang)(shang)(shang)(shang)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu);測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)完(wan)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)一(yi)(yi)(yi)(yi)、二(er)、三(san)(san)(san)(san)、四(si)和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)五(wu)(wu)后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)必(bi)須(xu)(xu)反(fan)(fan)(fan)應(ying)(ying)(ying)?20min?后(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)(hou)再(zai)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)六(liu)。3、反(fan)(fan)(fan)應(ying)(ying)(ying)過(guo)(guo)程中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)注(zhu)意(yi)(yi)避(bi)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)。4、若(ruo)(ruo)?NAD+測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0302,NADH?測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)△A(A2-A1)≤0.0222,說(shuo)明(ming)(ming)(ming)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)輔酶(mei)含(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)較(jiao)低,已低于(yu)(yu)(yu)(yu)(yu)(yu)檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限,可(ke)(ke)(ke)(ke)做(zuo)如(ru)(ru)(ru)下(xia)(xia)調(diao)整:(1)將(jiang)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)避(bi)光(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)(guang)靜(jing)置(zhi)(zhi)時間?20min?延長(chang)(chang)到?60min;(2)在(zai)(zai)(zai)(zai)(zai)(zai)(zai)(zai)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)階段增加(jia)(jia)(jia)(jia)(jia)(jia)(jia)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)量(liang)(liang)(liang)(liang)(liang)(liang),即取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)?0.2g?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)?0.2mL?樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)?1mL?提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)。5、由于(yu)(yu)(yu)(yu)(yu)(yu)每(mei)一(yi)(yi)(yi)(yi)個(ge)(ge)(ge)(ge)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)定(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)(ding)管(guan)(guan)(guan)(guan)需(xu)(xu)(xu)(xu)(xu)(xu)(xu)(xu)要(yao)(yao)(yao)(yao)設(she)一(yi)(yi)(yi)(yi)個(ge)(ge)(ge)(ge)對(dui)(dui)(dui)(dui)(dui)照(zhao)(zhao)(zhao)(zhao)(zhao)管(guan)(guan)(guan)(guan),本(ben)(ben)(ben)(ben)(ben)(ben)試(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)(shi)劑(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)(ji)盒(he)(he)(he)(he)(he)(he)(he)(he)?100?管(guan)(guan)(guan)(guan)保(bao)(bao)(bao)(bao)(bao)證(zheng)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)?48?個(ge)(ge)(ge)(ge)?NAD+或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)?NADH.NAD+和(he)(he)(he)(he)(he)(he)(he)(he)(he)(he)?NADH?含(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan):(一(yi)(yi)(yi)(yi)) NAD+含(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)標(biao)(biao)(biao)準條件(jian)下(xia)(xia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回(hui)歸曲(qu)線(xian)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?y = 0.1475x + 0.0302,R2 = 0.9978;其(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?NAD+濃(nong)(nong)度(du)(du)(du)(du)?nmol/mL1、血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)NAD+含(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)=[(△A -0.0302)÷0.1475×V1) ]÷(V3×V1÷V2)=135.6×(△A -0.0302)2、組(zu)(zu)織(zhi)(zhi)、細(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)胞中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NAD+含(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)(1)按(an)(an)(an)(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)蛋白濃(nong)(nong)度(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)NAD+(nmol/mg prot)=[(△A -0.0302)÷0.1475×V1)]÷(V1×Cpr)= 6.8×(△A -0.0302)÷Cpr(2)按(an)(an)(an)(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)鮮(xian)重(zhong)(zhong)(zhong)(zhong)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)NAD+(nmol/g?鮮(xian)重(zhong)(zhong)(zhong)(zhong))= [(△A -0.0302)÷0.1475×V1)]÷(W×V1÷V2)= 13.6×(△A -0.0302)÷W(3)按(an)(an)(an)(an)(an)(an)(an)細(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)胞密度(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)NAD+(nmol/104 cell)=[(△A -0.0302)÷0.1475×V1)]÷(500×V1÷V2)=0.027×(△A -0.0302)(二(er))NADH?含(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)標(biao)(biao)(biao)準條件(jian)下(xia)(xia)的(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)(de)回(hui)歸曲(qu)線(xian)為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?y = 0.1404x + 0.0222,R2 = 0.9976;其(qi)(qi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?y?為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)△A,x?為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?NADH?濃(nong)(nong)度(du)(du)(du)(du)?nmol/mL1、血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)(jiang))中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)NADH?含(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)(nmol/mL)= [ (△A -0.0222)÷0.1404×V1) ]÷(V3×V1÷V2)= 142.5×(△A -0.0222)2、組(zu)(zu)織(zhi)(zhi)、細(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)胞中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)?NADH?含(han)(han)量(liang)(liang)(liang)(liang)(liang)(liang)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)(1)按(an)(an)(an)(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)蛋白濃(nong)(nong)度(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)NADH (nmol/mg prot)= [ (△A -0.0222)÷0.1404×V1) ]÷(V1×Cpr)= 7.1×(△A -0.0222)÷Cpr(2)按(an)(an)(an)(an)(an)(an)(an)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)鮮(xian)重(zhong)(zhong)(zhong)(zhong)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)NADH (nmol/g?鮮(xian)重(zhong)(zhong)(zhong)(zhong))=[(△A -0.0222)÷0.1404×V1) ]÷(W×V1÷V2)= 14.2×(△A -0.0222)÷W(3)按(an)(an)(an)(an)(an)(an)(an)細(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)胞密度(du)(du)(du)(du)計(ji)(ji)(ji)(ji)(ji)算(suan)(suan)(suan)(suan)(suan)NADH (nmol/104 cell)= [ (△A -0.0222)÷0.1404×V1) ]÷(500×V1÷V2)=0.028×(△A -0.0222)V1:加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)反(fan)(fan)(fan)應(ying)(ying)(ying)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)系(xi)(xi)中(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)(zhong)樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji),0.02mL;V2:加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)提(ti)(ti)(ti)(ti)(ti)(ti)取(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)(qu)液(ye)(ye)(ye)(ye)(ye)(ye)(ye)體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji),2mL;V3:加(jia)(jia)(jia)(jia)(jia)(jia)(jia)入(ru)(ru)(ru)血(xue)(xue)(xue)清(qing)(qing)(qing)(qing)(qing)(qing)(漿(jiang)(jiang)(jiang)(jiang))體(ti)(ti)(ti)(ti)(ti)(ti)(ti)(ti)積(ji)(ji):0.1mL;Cpr:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)蛋白質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)濃(nong)(nong)度(du)(du)(du)(du),mg/mL; W:樣(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)(yang)本(ben)(ben)(ben)(ben)(ben)(ben)質(zhi)(zhi)(zhi)(zhi)(zhi)(zhi)量(liang)(liang)(liang)(liang)(liang)(liang),g;500:細(xi)(xi)(xi)(xi)(xi)(xi)胞或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)細(xi)(xi)(xi)(xi)(xi)(xi)菌(jun)(jun)總數(shu)(shu)(shu),500?萬。注(zhu)?意(yi)(yi):?檢(jian)(jian)(jian)(jian)(jian)(jian)(jian)(jian)測(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)(ce)限為(wei)(wei)(wei)(wei)(wei)(wei)(wei)(wei)?0.1nmol/mL?或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)?0.1nmol/g?鮮(xian)重(zhong)(zhong)(zhong)(zhong)?或(huo)(huo)(huo)(huo)(huo)(huo)(huo)(huo)?0.001nmol/mg prot